CN105961852A - Functional mulberry leaf extract and application thereof in biological feed - Google Patents
Functional mulberry leaf extract and application thereof in biological feed Download PDFInfo
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- CN105961852A CN105961852A CN201610273183.3A CN201610273183A CN105961852A CN 105961852 A CN105961852 A CN 105961852A CN 201610273183 A CN201610273183 A CN 201610273183A CN 105961852 A CN105961852 A CN 105961852A
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Abstract
The invention discloses a functional mulberry leaf extract and application thereof in a biological feed. The functional mulberry leaf extract is prepared by the following method: (a) mixing dried mulberry leaves with cortex phellodendri, performing pulverizing, performing hot reflux extraction with a 70-80% ethanol solution, merging extracting solutions, and performing concentration until ethanol taste does not exist so that an ethanol extraction concentrated solution is obtained; (b) diluting the ethanol extraction concentrated solution with water, performing extraction with petroleum ether, ethyl acetate and water-saturated n-butanol sequentially, and performing decompressed concentration so as to respectively obtain a petroleum ether extract, an ethyl acetate extract and an n-butanol extract; and (c) dissolving the n-butanol extract with water, performing filtration, enriching active components with D101 type macroporous resins, firstly rinsing 6-10 cylindrical volumes with 5-15% ethanol so as to remove large polar components, then eluting 8-12 cylindrical volumes with 70-80% ethanol, collecting 70-80% ethanol eluents, performing decompressed concentration, and performing spray drying so as to obtain the functional mulberry leaf extract, wherein the ratio of the parts by weight of the mulberry leaves to the parts by weight of the cortex phellodendri is (8-12) to 1. When the functional mulberry leaf extract provided by the invention is added to a feed, the estrus rate and the estrus and mated rate of cows can be greatly increased.
Description
Technical field
The present invention relates to the application in biological feedstuff of the functional plants extract, be specifically related to a kind of functional Folium Mori extract and
Its application in biological feedstuff.
Background technology
Folium Mori are the leaves of moraceae plants Mulberry, are the conventional food of silkworm, have another name called a Mulberry, Jing Sang, Fructus Mori tree, yellow wood leaf etc., China
Mulberry is extensively planted in various places, north and south, and mulberry yield enriches.The Major Foods of silkworm.Intact leaf in or width egg shape, be about 15 centimetres,
Wide about 10 centimetres, petiole is about 4 centimetres, and blade base is heart-shaped, the micro-point in top, and there are sawtooth, vein Mi Shengbai pubescence in edge.
The thicker yellow green of Lao Ye.Tender leaf is relatively thin, dirty-green.Matter is crisp easily, holds pricking the hand.Gas is light, and mildly bitter flavor is puckery.Medicinal it is generally acknowledged
After frost, the person's of adopting matter is good.Also it is conventional pig feed.
In the middle of cow feeding process, in cow feedstuff the nutrient substance such as proteins,vitamins,and minerals lack or
Person's nutrition is unbalanced, cause that cow uteri wall is thinning, uterine contraction weakens, ovary diminishes and in resting state, non-estrus.Logical
Cross and feed Chinese medicine extract and can improve the trophic level of cow and oestrus, ovulate.
Summary of the invention
It is an object of the invention to provide a kind of functional Folium Mori extract, after this functional Folium Mori extract adds in biological feedstuff
The reproductive performance of cow can be significantly improved.
Above-mentioned purpose is achieved by following technical solution:
A kind of functional Folium Mori extract, is prepared via a method which: dry Folium Mori and Cortex Phellodendri are mixed by (a), pulverize, and use
70～80% ethanol solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B () will step
Suddenly (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, subtracts
Pressure concentrates, and respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol extract water
Dissolve, filter, with D101 type macroporous resin enrichment active component, first with 5～15% alcohol flushing 6～10 column volumes remove greatly
Polar component, then with 70～80% ethanol elution 8～12 column volumes, collects 70～80% ethanol elution, concentrating under reduced pressure, spray
Mist is drying to obtain；Wherein, Folium Mori are 8～12:1 with the weight ratio of Cortex Phellodendri.
Further, described functional Folium Mori extract is prepared via a method which: dry Folium Mori and Cortex Phellodendri are mixed by (a),
Pulverize, with 75% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；(b)
By step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction,
Concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract is used
Water dissolution, filters, and with D101 type macroporous resin enrichment active component, first removes big pole with 8 column volumes of 10% alcohol flushing
Property composition, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；
Wherein, Folium Mori are 10:1 with the weight ratio of Cortex Phellodendri.
Further, described functional Folium Mori extract is prepared via a method which: dry Folium Mori and Cortex Phellodendri are mixed by (a),
Pulverize, with 70% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；(b)
By step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction,
Concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract is used
Water dissolution, filters, and with D101 type macroporous resin enrichment active component, first removes big pole with 10 column volumes of 5% alcohol flushing
Property composition, then with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；
Wherein, Folium Mori are 8:1 with the weight ratio of Cortex Phellodendri.
Further, described functional Folium Mori extract is prepared via a method which: dry Folium Mori and Cortex Phellodendri are mixed by (a),
Pulverize, with 80% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；(b)
By step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction,
Concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract is used
Water dissolution, filters, and with D101 type macroporous resin enrichment active component, first removes big pole with 6 column volumes of 15% alcohol flushing
Property composition, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；
Wherein, Folium Mori are 12:1 with the weight ratio of Cortex Phellodendri.
Above-mentioned functions Folium Mori extract is improving the application of cow reproduction aspect of performance.
A kind of biological feedstuff, containing above-mentioned functional Folium Mori extract.
Above-mentioned biological feedstuff is improving the application of cow reproduction aspect of performance.
Advantages of the present invention:
The functional Folium Mori extract that the present invention provides can significantly improve the reproductive performance of cow, compared with prior art has prominent
Substantive distinguishing features and significantly progressive.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.To the greatest extent
The present invention is explained in detail by pipe with reference to preferred embodiment, it will be understood by those within the art that, can be to the present invention
Technical scheme modify or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
In following embodiment, Folium Mori are the dried leaves of moraceae plants Mulberry；Cortex Phellodendri is the dry bark of rutaceae wampee, stripping
After taking bark, remove rough bark, dry.
Embodiment 1: the preparation of functional Folium Mori extract
A Folium Mori that 5kg is dried by () and Cortex Phellodendri mixing, pulverize, with 75% ethanol solution circumfluence distillation, and united extraction liquid,
It is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively
With petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate
Extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, by D101 type macroporous resin enrichment activity
Composition, first removes big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, receives
Collect 75% ethanol elution, concentrating under reduced pressure, be spray-dried and get final product；Wherein, Folium Mori are 10:1 with the weight ratio of Cortex Phellodendri.
Embodiment 2: the preparation of functional Folium Mori extract
A Folium Mori that 5kg is dried by () and Cortex Phellodendri mixing, pulverize, with 70% ethanol solution circumfluence distillation, and united extraction liquid,
It is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively
With petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate
Extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, by D101 type macroporous resin enrichment activity
Composition, first removes big polar component with 10 column volumes of 5% alcohol flushing, then with 12 column volumes of 70% ethanol elution, receives
Collect 70% ethanol elution, concentrating under reduced pressure, be spray-dried and get final product；Wherein, Folium Mori are 8:1 with the weight ratio of Cortex Phellodendri.
Embodiment 3: the preparation of functional Folium Mori extract
A Folium Mori that 5kg is dried by () and Cortex Phellodendri mixing, pulverize, with 80% ethanol solution circumfluence distillation, and united extraction liquid,
It is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively
With petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate
Extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, by D101 type macroporous resin enrichment activity
Composition, first removes big polar component with 6 column volumes of 15% alcohol flushing, then with 8 column volumes of 80% ethanol elution, collects
80% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, Folium Mori are 12:1 with the weight ratio of Cortex Phellodendri.
Embodiment 4: the preparation of functional Folium Mori extract
A Folium Mori that 5kg is dried by () and Cortex Phellodendri mixing, pulverize, with 75% ethanol solution circumfluence distillation, and united extraction liquid,
It is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively
With petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate
Extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, by D101 type macroporous resin enrichment activity
Composition, first removes big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, receives
Collect 75% ethanol elution, concentrating under reduced pressure, be spray-dried and get final product；Wherein, Folium Mori are 8:1 with the weight ratio of Cortex Phellodendri.
Embodiment 5: the preparation of functional Folium Mori extract
A Folium Mori that 5kg is dried by () and Cortex Phellodendri mixing, pulverize, with 75% ethanol solution circumfluence distillation, and united extraction liquid,
It is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively
With petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate
Extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, by D101 type macroporous resin enrichment activity
Composition, first removes big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, receives
Collect 75% ethanol elution, concentrating under reduced pressure, be spray-dried and get final product；Wherein, Folium Mori are 12:1 with the weight ratio of Cortex Phellodendri.
Embodiment 6: the contrast of embodiment 1, Folium Mori are 7:1 with the weight ratio of Cortex Phellodendri
A Folium Mori that 5kg is dried by () and Cortex Phellodendri mixing, pulverize, with 75% ethanol solution circumfluence distillation, and united extraction liquid,
It is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively
With petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate
Extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, by D101 type macroporous resin enrichment activity
Composition, first removes big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, receives
Collect 75% ethanol elution, concentrating under reduced pressure, be spray-dried and get final product；Wherein, Folium Mori are 7:1 with the weight ratio of Cortex Phellodendri.
Embodiment 7: the contrast of embodiment 1, Folium Mori are 13:1 with the weight ratio of Cortex Phellodendri
A Folium Mori that 5kg is dried by () and Cortex Phellodendri mixing, pulverize, with 75% ethanol solution circumfluence distillation, and united extraction liquid,
It is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively
With petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate
Extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, by D101 type macroporous resin enrichment activity
Composition, first removes big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, receives
Collect 75% ethanol elution, concentrating under reduced pressure, be spray-dried and get final product；Wherein, Folium Mori are 13:1 with the weight ratio of Cortex Phellodendri.
Embodiment 8: effect example, the impact on cow reproduction performance
One, materials and methods
1, test specimen: prepare extract 1～7 according to embodiment 1～7 respectively.
2, method
2.1 experimental animals, packet and method for breeding
Though selecting non-reproductive system disease, non-reproductive system deformity but because malnutrition causes ovary to be resting state, non-estrus
Chinese XIMEN Qin 96, be randomly divided into 8 groups, matched group 12, test group 1～7 often organizes 12.Matched group is raised
Feeding basis forage, test group 1～7 adds the extract 1～7 of 220g/t respectively on the basis of the forage of basis respectively.
Test 1 month by a definite date.
2.2 statistical indicator
Respectively organize estrus of cow quantity in the statistical test time limit and oestrus by dosage, calculating each group of estrus of cow rate with oestrusing and joined rate.
Two, result
As can be seen from Table 1, compared with matched group, test group 1,4,5 estrus of cow rate and oestrus and joined rate and be significantly increased,
Significant difference (P ＜ 0.05)；
Test group 6 with 7 compared with matched group, estrus of cow rate and oestrus and joined rate and there was no significant difference (P ＞ 0.05).
Specifically it is shown in Table 1.
Estrus of cow rate is affected by joining rate by table 1 with oestrusing
Test group 2 is similar to the effect of test group 4 and test group 5 with the effect of test group 3, without significant difference.
Result shows, the functional Folium Mori extract that the present invention provides can significantly improve estrus of cow rate and be joined rate with oestrusing.
The above-mentioned effect of extract of the present invention is relevant with the weight ratio of Folium Mori and Cortex Phellodendri.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.
It will be understood by those within the art that, technical scheme can be modified or equivalent, and not take off
Essence and protection domain from technical solution of the present invention.
Claims (7)
1. a functional Folium Mori extract, it is characterised in that be prepared via a method which: (a) is by dry Folium Mori and Huang
Cypress mixes, and pulverizes, and with 70～80% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into that to obtain ethanol extraction without alcohol taste dense
Contracting liquid；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water saturation successively
N-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c)
N-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 5～15% alcohol flushing 6～10
Individual column volume removes big polar component, then with 70～80% ethanol elution 8～12 column volumes, collection 70～80% ethanol elution,
Concentrating under reduced pressure, is spray-dried and get final product；Wherein, Folium Mori are 8～12:1 with the weight ratio of Cortex Phellodendri.
Functional Folium Mori extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry Folium Mori and Cortex Phellodendri are mixed, pulverizes, with 75% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into without alcohol taste
Obtain ethanol extraction concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, second successively
Acetoacetic ester and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and positive fourth
Alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 10%
8 column volumes of alcohol flushing remove big polar component, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, Folium Mori are 10:1 with the weight ratio of Cortex Phellodendri.
Functional Folium Mori extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry Folium Mori and Cortex Phellodendri are mixed, pulverizes, with 70% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into without alcohol taste
Obtain ethanol extraction concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, second successively
Acetoacetic ester and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and positive fourth
Alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 5%
10 column volumes of alcohol flushing remove big polar component, then with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, Folium Mori are 8:1 with the weight ratio of Cortex Phellodendri.
Functional Folium Mori extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry Folium Mori and Cortex Phellodendri are mixed, pulverizes, with 80% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into without alcohol taste
Obtain ethanol extraction concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, second successively
Acetoacetic ester and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and positive fourth
Alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 15%
6 column volumes of alcohol flushing remove big polar component, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution,
Concentrating under reduced pressure, is spray-dried and get final product；Wherein, Folium Mori are 12:1 with the weight ratio of Cortex Phellodendri.
5. the arbitrary described functional Folium Mori extract of Claims 1 to 4 is in the application improving cow reproduction aspect of performance.
6. a biological feedstuff, it is characterised in that containing the arbitrary described functional Folium Mori extract of Claims 1 to 4.
7. the biological feedstuff described in claim 6 is improving the application of cow reproduction aspect of performance.
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| CN201610273183.3A CN105961852A (en) | 2016-04-27 | 2016-04-27 | Functional mulberry leaf extract and application thereof in biological feed |
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| CN201610273183.3A CN105961852A (en) | 2016-04-27 | 2016-04-27 | Functional mulberry leaf extract and application thereof in biological feed |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512580A (en) * | 2011-12-27 | 2012-06-27 | 李继红 | Chinese medicinal granular preparation for detoxification and purification of uteruses of cows |
| CN103141679A (en) * | 2013-03-06 | 2013-06-12 | 魏放 | Composite sheep feed containing Chinese medicinal herbs |
-
2016
- 2016-04-27 CN CN201610273183.3A patent/CN105961852A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512580A (en) * | 2011-12-27 | 2012-06-27 | 李继红 | Chinese medicinal granular preparation for detoxification and purification of uteruses of cows |
| CN103141679A (en) * | 2013-03-06 | 2013-06-12 | 魏放 | Composite sheep feed containing Chinese medicinal herbs |
Non-Patent Citations (2)
| Title |
|---|
| 李仲武: "黄柏车前汤治母牛化脓性子宫内膜炎", 《疫病防治》 * |
| 郭建军，等: "饲料中添加桑叶对育肥牛增重的影响", 《当代畜牧》 * |
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