CN105994940A - Biological active protein feed and preparation method thereof - Google Patents
Biological active protein feed and preparation method thereof Download PDFInfo
- Publication number
- CN105994940A CN105994940A CN201610345696.0A CN201610345696A CN105994940A CN 105994940 A CN105994940 A CN 105994940A CN 201610345696 A CN201610345696 A CN 201610345696A CN 105994940 A CN105994940 A CN 105994940A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- hours
- feed
- feedstuff
- bioactive protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention belongs to the field of feed, and concretely discloses biological active protein feed. The feed is prepared from the following raw material ingredients including fruit slag, oil slag, bran, urea and a fermentation bacterium agent, wherein the fermentation bacterium agent is a composite bacterium agent of mycete, saccharomycetes, spore bacteria and lactobacilli. A preparation method of the biological active protein feed comprises the following steps of firstly preparing a fermentation culture medium; performing high-pressure steam sterilization on the fermentation culture medium; then, sequentially inoculating a composite bacterium agent of a mycete solid spore bacterium agent, activated saccharomycetes, spore bacteria and lactobacilli onto the cooled sterilization fermentation culture medium; fermenting aerobic fermentation products; finally transmitting the aerobic fermentation products into a sealed anaerobic fermentation cabin; performing anaerobic fermentation for 5 days; performing blast blowing heating drying on the obtained product; obtaining the feed. The feed and the preparation method have the advantages that the metabolic capability of the fermentation bacterium agent can be sufficiently realized; the production cost is low; the operation is simple and convenient; coarse feed can be effectively modified into biological active protein feed.
Description
Technical field
The present invention relates to field of fodder, be specifically related to a kind of bioactive protein feedstuff and preparation method thereof.
Background technology
Along with people's growth to the demand of livestock products, animal husbandry have also been obtained fast development, but people
Mouth increases and limits the grain yield of China with cultivated land area, causes people and animals' contradiction, especially
China's present stage productive structure of animal husbandry determines that it is very big to the dependency of grain, albumen feedstuff and energy
Raw material all suffers from situation in short supply, and annual domestic annual requirement reaches 80,000,000 tons, and breach reaches 4000
More than ten thousand tons, and protein feed raw material price goes up year after year and had a strong impact on China's feed industry and aquaculture
Development, the simple development relying on conventional feed can not meet the most far away animal husbandry.Therefore, grain is improved
Food conversion ratio and efficiency of feed utilization, research novel fodder additive and the unconventional raw material resources of exploitation,
The sustainable feed industry of new high-efficiency is set up in development has become the key subjects developed animal husbandry.
At present, along with the development of fermentation technique, microbial fermentation technology produce protein feeds and
The feed additives such as aminoacid, vitamin, enzyme preparation and microorganism formulation, in exploitation novel fodder resource
In be used widely, make fermentation technique get growing concern in feedstuff industry and pay attention to.2013
The definition of biological feedstuff is by biological feedstuff exploitation in November in the year National Engineering Research Centre Committee of Experts: raw
Thing feedstuff refers to feedstuff and feed additive as object, with genetic engineering, protein engineering, fermentation work
The modern biotechnologies such as journey are means, utilize novel fodder resource and the feedstuff of microbial project fermentation exploitation
The general name of additive.Biological feedstuff kind mainly includes fodder enzyme preparation, additive for microbe feedstuff, merit
Can property protein peptide, functional amino, organic trace element, plant and microorganism extracts, prebiotics,
Fermentation and enzymolysis feed and other biological technology Related product.It is mould that biological fermentation feed commonly uses microorganism
Bacterium, yeast, lactic acid bacteria and sporeformer.The production technology of biological fermentation feed is mainly: one, solid
Superficial fermentation, due to solid fermentation utilization is the water insoluble solid culture medium with certain humidity,
There is water saving, energy-conservation unique advantage, belong to clearer production technology progressively obtain countries in the world attention and
Become the focus of research；Two, big tank solution fermentation, is primarily adapted for use in the aerobic microbiologicals such as bacillus cereus
Production, but each strain produce concrete technology different, this method is applicable to industrialized production, just
In sterile working, but cost is high；Three, anaerobic fermentation, is primarily adapted for use in the absolute anaerobe such as bacillus bifidus
Production.
Research shows, the use in feedstuff of the medicine such as antibiotic, hormone, had a strong impact on livestock products and
The quality and safety of aquatic products, develop and promote one can effectively substitute antibiotics, hormone, preservative,
The bioactive protein feedstuff again with multiple actions such as health care growth promotions has become the focus of current research.
Therefore, the probiotic bacteria such as increasing sporeformer and lactic acid bacteria starts to be applied to biological fermentation feed, and carefully
Bacterium, yeast and mold fermentation become new trend.But traditional solid state surface fermentation technology is based on consumption
The fermentation process of oxygen animalcule, it is impossible to give full play to mycete, yeast and antibacterial and improving the same of character of feed
Shi Tigao feedstuff commodity value；Therefore, in the urgent need to innovation and the optimization of fermentation technology, solid-state is realized
Fermenting and producing high-quality biological active protein feed, and finally realize the abundant exploitation of unconventional water resources resource
Utilize and grain conversion rate, being greatly improved of efficiency of feed utilization.
Summary of the invention
For problems of the prior art, it is an object of the invention to provide a kind of biological activity protein
Feedstuff and preparation method thereof, the method production cost is low, easy and simple to handle, it is possible to give full play to mycete, ferment
The microbial inoculum metabolic capacities such as female bacterium, sporeformer and lactic acid bacteria, effectively modification roughage is that biological activity protein is raised
Material.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
(1) a kind of bioactive protein feedstuff, it is characterised in that include following material composition: marc,
The dregs of fat, wheat bran, carbamide；Also include fermenting agent；
Described fermenting agent is the composite bacteria agent capable of mycete, yeast, sporeformer and lactic acid bacteria.
Further, the mass percent of described material composition is: marc 75%～78%, the dregs of fat
13%～15%, wheat bran 5%～7%, carbamide 2%～3%.
Further, described mycete is seeded in described material composition with the form of mycete solid-state spore microbial inoculum,
Inoculum concentration is the 0.5%～0.6% of described material composition.
Further, described yeast is seeded in described material composition with the form of activated yeast, connects
The amount of kind is the 0.5%～0.6% of described material composition.
Further, described sporeformer is described material composition with the inoculum concentration of the composite bacteria agent capable of lactic acid bacteria
0.5%～0.6%；Wherein, described sporeformer is 2:1～2:1.5 with the mass ratio of lactic acid bacteria.
Further, described mycete is Aspergillus niger, and described yeast is Angel saccharomyces cerevisiae.
Further, the water content of described bioactive protein feedstuff is less than or equal to 18%.
(2) preparation method of a kind of bioactive protein feedstuff, it is characterised in that comprise the following steps:
Step 1, prepares fermentation medium: weigh the marc of described material composition, oil according to claim 2
Slag, wheat bran and carbamide, add water and inorganic salt mix and blend, and pH is natural, described material composition and water
Mass ratio be 1:1.2～1:1.3；
Step 2, carries out high pressure steam sterilization by fermentation medium；
Step 3, after being cooled down by the fermentation medium after sterilizing, is placed in solid fermentation storehouse, and it is solid to inoculate mycete
State spore microbial inoculum, temperature be 30 DEG C～35 DEG C, humidity be to ferment 34 hours～38 under conditions of 50%～60%
Hour；
Step 4, after mold fermentation terminates, the yeast of inoculation activation, continue temperature be 30 DEG C～35 DEG C,
Under conditions of humidity is 50%～60%, yeast and mold is fermented altogether 45 hours～50 hours, every 4 hours
～5 hours aerators stir 2 minutes～5 minutes；
Step 5, after yeast and mold fermentation ends altogether, accesses sporeformer and the composite bacteria agent capable of lactic acid bacteria,
It is 32 DEG C～35 DEG C in temperature, under conditions of humidity is 55%～60%, ferments 24 hours～30 hours, every 4
Hour～5 hours aerators stir 2 minutes～5 minutes, obtain aerobic fermentation product；
Step 6, is finally placed in the anaerobic fermentation storehouse of closing, anaerobic fermentation 4～5 days by aerobic fermentation product；
Step 7, after anaerobic fermentation is terminated, by dried for the product blast heating obtained, obtains biological activity
Albumen feedstuff.
Further, in step 1, described inorganic salt comprises: KH2PO42.8～3.2g/kg, CaCl2
0.45～0.55g/kg, MgSO4·7H2O 0.45～0.55g/kg, FeSO4·7H2O 7.4～7.6mg/kg,
MnSO4·H2O 2.4～2.6mg/kg, ZnSO4·7H2O 3.5～3.7mg/kg.
Further, in step 2, the inoculum concentration of described mycete solid-state spore microbial inoculum is described food ingredient
0.5～0.6%.
Further, in step 2, described mycete solid-state spore microbial inoculum is in going out by mycotic spore suspension inoculation
Water content after bacterium is in the bran mass of 50%, cultivates 4 days～6 days gained for 28 DEG C～31 DEG C.
Further, in step 3, the saccharomycetic inoculum concentration of described activation is described food ingredient
0.5%～0.6%.
Further, in step 3, the activation of Angel saccharomyces cerevisiae be yeast is inoculated in 4% brown sugar
In aseptic aqueous solution, 30 DEG C, cultivate 24 hours under the conditions of 1000rpm.
Further, in step 4, the inoculum concentration of the composite bacteria agent capable of described sporeformer and lactic acid bacteria be described in join
The 0.5%～0.6% of material composition, wherein, described sporeformer is 2:1～2:1.5 with the mass ratio of lactic acid bacteria.
Further, in step 6, the ventilation temperature that described blast heating is dried is 75 DEG C～80 DEG C, material
Temperature is 28 DEG C～30 DEG C, and the time is 4 hours～4.5 hours.
The fermentation medium selection marc of the present invention, the reasonable compatibility dregs of fat, wheat bran, carbamide and inorganic salt,
For the growth reasonable supplement organic and inorganic available nitrogen source of microorganism, increase base protein in raw material simultaneously and contain
Amount, the interpolation of inorganic salt can promote the generation of fungus exoenzyme.
Yeast and mold is creatively successively inoculated by the present invention in batches, first allows mycete at fermentation medium
Middle growth, utilizes the enzymes such as its cellulase produced, pectase macromole such as degraded cellulose, pectin alive
Nutrient is reducing sugar, the galacturonic acid etc. easily utilized, and provides nutrition for saccharomycetic Fast-propagation
Material, beneficially inorganic nitrogen, to the transformation of organic nitrogen, promote protein accumulation；Yeast is to Portugal simultaneously
The utilization of the enzymatic hydrolysate such as grape sugar, slow down feedback mechanism to a certain extent and presses down mycete extracellular protease secretion
System so that tunning has the enzymatic activity high of spectrum.
Meanwhile, aerobic fermentation and anaerobic fermentation are combined by the present invention, make sending out of fungal inoculant and antibacterial microbial inoculum
Ferment benefit reaches maximum, and antibacterial microbial inoculum aerobic fermentation, after 24 hours, enters anaerobic stages, now, antibacterial
Adapt to fermentation substrate and had the accumulation of quantity.At anaerobic stages, although the activity of yeast and mold
It is suppressed, but its exoenzyme produced is at a suitable temperature, continue to play zymolysis, extend
Modification effect to raw material, adds the nutrients ratio easily absorbed by animal in feedstuff；Meanwhile, with
The carrying out of anaerobic fermentation, anaerobism degree gradual change is strengthened, and produces spore for sporeformer and provides condition and time,
Ensure that the highly active existence of sporeformer；Lactic acid accelerates metabolism at anaerobic stages, beneficially metabolite
Accumulation, add palatability and the prebiotic effect of feedstuff.
It addition, the present invention uses low temperature forced air drying, by controlling blast temperature and temperature of charge, can be real
The Quick-air-drying of existing feedstuff, not only improves storage and the transport of biological feedstuff, it is to avoid the pollution of feedstuff and change
Matter, the enzyme the most farthest protecting bioactive feed is lived and the probiotic bacteria such as sporeformer, lactic acid bacteria
Activity and content.
The present invention uses the method for solid fermentation, reduces fermentation costs, improves operability, reduces
Environmental pollution, the most beneficially exploitation of solid-state feed raw material.
Detailed description of the invention
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but the skill of this area
Art personnel are it will be appreciated that the following example is merely to illustrate the present invention, and are not construed as limiting the present invention's
Scope.
Embodiment 1
(1) strain and source
The mycete of the bioactive protein feedstuff of the present embodiment is aspergillus niger MHQ1, big by northwest agriculture and forestry science and technology
Learn the preservation of Microbiological Lab of resource environment institute；Yeast is Angel saccharomyces cerevisiae, buys in Angel
Yeast limited company；It is limited that the compound bacteria of lactic acid bacteria and sporeformer buys the azure biotechnology in Shandong
Company.
(2) preparation of microbial inoculum and activation
The preparation of aspergillus niger MHQ1 solid-state spore preparation: by the aspergillus niger MHQ1 PDA of preservation in test tube
Solid slope activates, and by the slant pore sterilized water eluting of activation, makes a small amount of aspergillus niger MHQ1
Spore suspension, is inoculated in above-mentioned aspergillus niger MHQ1 spore suspension in PDA triangular flask solid medium, training
Support to growing a large amount of spore, then use sterilized water eluting, obtain a large amount of aspergillus niger MHQ1 spore suspension.Then
Use tissue culture bottle solid fermentation, precise 50g wheat bran, add 60mL tap water, stir, 121 DEG C
Sterilizing 30 minutes, accesses 5mL aspergillus niger MHQ1 spore suspension, then in 30 DEG C of constant temperature of culturing room after cooling
Cultivate 5 days.
The activation of Angel saccharomyces cerevisiae: Angel saccharomyces cerevisiae microbial inoculum is inoculated in the brown sugar equipped with 7.5kg 4%
In aseptic aqueous solution, 30 DEG C of 100rpm stir culture 24 hours.
The activation of the composite bacteria agent capable of sporeformer and lactic acid bacteria: composite bacteria agent capable is inoculated in 4% brown sugar aseptic water-soluble
In liquid, 35 DEG C of 100rpm stir culture 24 hours.
(3) preparation of fermentation medium
The gross mass of marc, the dregs of fat, wheat bran and carbamide and the mass ratio of water are 1:1.2, wherein, marc 77%,
The dregs of fat 15%, wheat bran 5%, carbamide 3%；With the gross mass of marc, the dregs of fat, wheat bran and carbamide for 1kg it is
Benchmark, inorganic salt consist of KH2PO4 3g/kg、CaCl2 0.5g/kg、MgSO4·7H2O 0.5g/kg、
FeSO4·7H2O 7.5mg/kg、MnSO4·H2O 2.5mg/kg、ZnSO4·7H2O 3.6mg/kg.Carbamide
And inorganic salt adds in fermentation medium the most in form of an aqueous solutions.
(4) sweat:
Step 1, prepares fermentation medium, and fermentation medium is carried out high pressure steam sterilization；
Step 2, after being cooled down by the fermentation medium after sterilizing, is sent to solid fermentation storehouse on fluid bed,
And inoculated aspergillus niger MHQ1 solid-state spore microbial inoculum, 30 DEG C～35 DEG C, under conditions of humidity is 50%～60%
Ferment 34～38 hours；
Step 3, after aspergillus niger MHQ1 fermentation ends, the Angel saccharomyces cerevisiae of inoculation activation, continues
Under conditions of temperature is 30 DEG C～35 DEG C, humidity is 50%～60%, ferment that aspergillus niger MHQ1 and Angel are made wine
Female bacterium is fermented 48 hours altogether, and within every 4 hours, aerator stirs 2 minutes；
After step 4, aspergillus niger MHQ1 and Angel saccharomyces cerevisiae fermentation ends altogether, access sporeformer and breast
The composite bacteria agent capable of acid bacterium, is 32 DEG C～35 DEG C in temperature, under conditions of humidity is 55%～60%, ferments 24
Hour, within every 4 hours, aerator stirs 5 minutes, obtains aerobic fermentation product；
Step 5, is finally sent to the anaerobic fermentation storehouse closed, anaerobic fermentation 5 days by aerobic fermentation product；
Step 6, after anaerobic fermentation is terminated, by dried for the product blast heating obtained, ventilation temperature is
78 DEG C, temperature of charge is 27 DEG C, dries 4 hours, obtains bioactive protein feedstuff.
The water content of the bioactive protein feedstuff of gained is 16.3%, dried feed nutrient and active component
Content be: crude protein content 27.8%, carboxymethylcelluloenzyme enzyme live 5389U/g, pectase work is
1176U/g, content of lactic acid bacteria is 1.7 × 108Cfu/g, sporeformer content is 6.7 × 107Cfu/g, yeast contains
Amount is 4.5 × 107cfu/g。
Embodiment 2
(1) strain and source
The mycete of the bioactive protein feedstuff of the present embodiment is aspergillus niger A8, by Xibei Univ. of Agricultural & Forest Science & Technology
The preservation of Microbiological Lab of resource environment institute；Yeast is Angel saccharomyces cerevisiae, buys in Angel ferment
Female limited company；The compound bacteria of lactic acid bacteria and sporeformer buys the limited public affairs of azure biotechnology in Shandong
Department.
(2) preparation of microbial inoculum and activation
The preparation of aspergillus niger A8 solid-state spore preparation: by oblique for the aspergillus niger A8 PDA solid of preservation in test tube
Face activates, and by the slant pore sterilized water eluting of activation, makes a small amount of aspergillus niger A8 spore suspension,
Above-mentioned aspergillus niger A8 spore suspension is inoculated in PDA triangular flask solid medium, cultivates to growing a large amount of
Spore, then use sterilized water eluting, obtain a large amount of aspergillus niger MHQ1 spore suspension.Then use tissue culture bottle solid
State is fermented, precise 50g wheat bran, adds 60mL tap water, stirs, 121 DEG C of sterilizings 30 minutes,
5mL aspergillus niger A8 spore suspension is accessed, then in 30 DEG C of constant temperature culture of culturing room 5 days after cooling.
The activation of Angel saccharomyces cerevisiae: Angel saccharomyces cerevisiae microbial inoculum is inoculated in the brown sugar equipped with 7.5kg 4%
In aseptic aqueous solution, 30 DEG C of 100rpm stir culture 24 hours.
The activation of the composite bacteria agent capable of sporeformer and lactic acid bacteria: composite bacteria agent capable is inoculated in 4% brown sugar aseptic water-soluble
In liquid, 35 DEG C of 100rpm stir culture 24 hours.
(3) preparation of fermentation medium
The gross mass of marc, the dregs of fat, wheat bran and carbamide and the mass ratio of water are 1:1.2, wherein, marc 77%,
The dregs of fat 15%, wheat bran 5%, carbamide 3%；With the gross mass of marc, the dregs of fat, wheat bran and carbamide for 1kg it is
Benchmark, inorganic salt consist of KH2PO4 3g/kg、Cacl2 0.5g/kg、MgSO4·7H2O 0.5g/kg、
FeSO4·7H2O 7.5mg/kg、MnSO4·H2O 2.5mg/kg、ZnSO4·7H2O 3.6mg/kg.Carbamide
And inorganic salt adds in fermentation medium in form of an aqueous solutions.
(4) sweat:
Step 1, prepares fermentation medium, and fermentation medium is carried out high pressure steam sterilization；
Step 2, after being cooled down by the fermentation medium after sterilizing, is sent to solid fermentation storehouse on fluid bed,
And inoculated aspergillus niger A8 solid-state spore microbial inoculum, temperature be 30 DEG C～35 DEG C, humidity be the bar of 50%～60%
Part bottom fermentation 36 hours；
Step 3, after aspergillus niger A8 fermentation ends, the Angel saccharomyces cerevisiae of inoculation activation, is 30 DEG C in temperature
～35 DEG C, under conditions of humidity is 50%～60%, aspergillus niger A8 and Angel saccharomyces cerevisiae are fermented altogether 48
Hour, within every 4 hours, aerator stirs 5 minutes；
After step 4, aspergillus niger A8 and Angel saccharomyces cerevisiae fermentation ends altogether, access sporeformer and lactic acid bacteria
Composite bacteria agent capable, be 32 DEG C～35 DEG C in temperature, under conditions of humidity is 55%～60%, ferment 24 hours,
Within every 4 hours, aerator stirs 5 minutes, obtains aerobic fermentation product；
Step 5, is finally sent to the anaerobic fermentation storehouse closed, anaerobic fermentation 5 days by aerobic fermentation product；
Step 6, after anaerobic fermentation is terminated, by dried for the product blast heating obtained, ventilation temperature is
80 DEG C, temperature of charge is 27 DEG C, dries 4 hours, obtains bioactive protein feedstuff.
The water content of the bioactive protein feedstuff of gained is 14.7%, dried feed nutrient and active component
Content be: crude protein content 24.6%, carboxymethylcelluloenzyme enzyme lives 4957U/g, and pectase is lived as 973U/g,
Content of lactic acid bacteria is 1.2 × 108Cfu/g, sporeformer content is 4.6 × 107Cfu/g, yeast content is 2.9 × 107
cfu/g。
Although, the present invention has been made by this specification the most with a general description of the specific embodiments detailed
Description, but on the basis of the present invention, it can be made some modifications or improvements, this is to art technology
It is apparent from for personnel.Therefore, these done without departing from theon the basis of the spirit of the present invention are repaiied
Change or improve, belonging to the scope of protection of present invention.
Claims (10)
1. a bioactive protein feedstuff, it is characterised in that include following material composition: marc, oil
Slag, wheat bran, carbamide；Also include fermenting agent；
Described fermenting agent is the composite bacteria agent capable of mycete, yeast, sporeformer and lactic acid bacteria.
Bioactive protein feedstuff the most according to claim 1, it is characterised in that described raw material becomes
The mass percent divided is: marc 75%～78%, the dregs of fat 13%～15%, wheat bran 5%～7%, carbamide 2%～3%.
Bioactive protein feedstuff the most according to claim 1, it is characterised in that described mycete with
The form of mycete solid-state spore microbial inoculum is seeded in described material composition, and inoculum concentration is described material composition
0.5%～0.6%.
Bioactive protein feedstuff the most according to claim 1, it is characterised in that described yeast
Being seeded in described material composition with the form of activated yeast, inoculum concentration is described material composition
0.5%～0.6%.
Bioactive protein feedstuff the most according to claim 1, it is characterised in that described sporeformer
With 0.5%～0.6% that the inoculum concentration of the composite bacteria agent capable of lactic acid bacteria is described material composition；Wherein, described spore
Bacterium is 2:1～2:1.5 with the mass ratio of lactic acid bacteria.
Bioactive protein feedstuff the most according to claim 1, it is characterised in that described biological alive
Property albumen feedstuff water content less than or equal to 18%.
7. the preparation method of a bioactive protein feedstuff, it is characterised in that comprise the following steps:
Step 1, prepares fermentation medium: weigh the marc of described material composition, oil according to claim 2
Slag, wheat bran and carbamide, add water and inorganic salt mix and blend, and pH is natural, described material composition and water
Mass ratio be 1:1.2～1:1.3；
Step 2, carries out high pressure steam sterilization by fermentation medium；
Step 3, after being cooled down by the fermentation medium after sterilizing, is placed in solid fermentation storehouse, and it is solid to inoculate mycete
State spore microbial inoculum, temperature be 30 DEG C～35 DEG C, humidity be to ferment 34 hours～38 under conditions of 50%～60%
Hour；
Step 4, after mold fermentation terminates, the yeast of inoculation activation, continue temperature be 30 DEG C～35 DEG C,
Under conditions of humidity is 50%～60%, yeast and mold is fermented altogether 45 hours～50 hours, every 4 hours
～5 hours aerators stir 2 minutes～5 minutes；
Step 5, after yeast and mold fermentation ends altogether, accesses sporeformer and the composite bacteria agent capable of lactic acid bacteria,
It is 32 DEG C～35 DEG C in temperature, under conditions of humidity is 55%～60%, ferments 24 hours～30 hours, every 4
Hour～5 hours aerators stir 2 minutes～5 minutes, obtain aerobic fermentation product；
Step 6, is finally placed in the anaerobic fermentation storehouse of closing, anaerobic fermentation 4～5 days by aerobic fermentation product；
Step 7, after anaerobic fermentation is terminated, by dried for the product blast heating obtained, obtains biological activity
Albumen feedstuff.
The preparation method of bioactive protein feedstuff the most according to claim 7, it is characterised in that
In step 1, described inorganic salt comprises: KH2PO42.8～3.2g/kg, CaCl20.45～0.55g/kg,
MgSO4·7H2O 0.45～0.55g/kg, FeSO4·7H2O 7.4～7.6mg/kg, MnSO4·H2O
2.4～2.6mg/kg, ZnSO4·7H2O 3.5～3.7mg/kg.
The preparation method of bioactive protein feedstuff the most according to claim 7, it is characterised in that
In step 2, described mycete solid-state spore microbial inoculum is by mycotic spore suspension inoculation water content after sterilizing
It is in the bran mass of 50%, cultivates 4 days～6 days gained for 28 DEG C～31 DEG C.
The preparation method of bioactive protein feedstuff the most according to claim 7, it is characterised in that
In step 6, the ventilation temperature that described blast heating is dried is 75 DEG C～80 DEG C, and temperature of charge is 28 DEG C
～30 DEG C, the time is 4 hours～4.5 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610345696.0A CN105994940B (en) | 2016-05-23 | 2016-05-23 | Bioactive protein feed and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610345696.0A CN105994940B (en) | 2016-05-23 | 2016-05-23 | Bioactive protein feed and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105994940A true CN105994940A (en) | 2016-10-12 |
| CN105994940B CN105994940B (en) | 2020-07-07 |
Family
ID=57097059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610345696.0A Active CN105994940B (en) | 2016-05-23 | 2016-05-23 | Bioactive protein feed and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105994940B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107889954A (en) * | 2017-11-28 | 2018-04-10 | 佛山宝来迪生物科技有限公司 | A kind of pineapple peel viable bacteria feedstuff for live pigs and its preparation and feeding method |
| CN108157588A (en) * | 2017-12-23 | 2018-06-15 | 董晓 | A kind of preparation method of protein feed |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550835A (en) * | 2012-02-13 | 2012-07-11 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Microbe feed additive and preparation method thereof |
| CN103250874A (en) * | 2013-06-08 | 2013-08-21 | 潘李亮 | Preparation method of apple pomace fermented feed |
-
2016
- 2016-05-23 CN CN201610345696.0A patent/CN105994940B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550835A (en) * | 2012-02-13 | 2012-07-11 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Microbe feed additive and preparation method thereof |
| CN103250874A (en) * | 2013-06-08 | 2013-08-21 | 潘李亮 | Preparation method of apple pomace fermented feed |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107889954A (en) * | 2017-11-28 | 2018-04-10 | 佛山宝来迪生物科技有限公司 | A kind of pineapple peel viable bacteria feedstuff for live pigs and its preparation and feeding method |
| CN108157588A (en) * | 2017-12-23 | 2018-06-15 | 董晓 | A kind of preparation method of protein feed |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105994940B (en) | 2020-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102160595B (en) | Preparation process of complex microorganism fermented active feed | |
| CN104996722B (en) | A kind of method of the step combined ferment feed of multi-cultur es two | |
| CN101139560B (en) | Process for producing feeding micro-ecological preparation by solid fermentation of brewery mash with various bacterium and fermentation culture medium thereof | |
| CN102132762B (en) | Straw feed and preparation method thereof | |
| CN101433270B (en) | Preparation method of vegetable seed protein feed | |
| CN104186957A (en) | Method for improving forage nutritional value of cottonseed meal through microbial fermentation | |
| CN102599351A (en) | Bio-enzyme and microorganism containing compound feed additive and preparation process thereof | |
| CN100391357C (en) | Bacilli fungi mixed fermentation for producing microbial formulation and compound enzyme feed additive | |
| CN106520584B (en) | Saccharomycete and lactic acid bacteria co-culture with culture medium and preparation method thereof | |
| CN104381686A (en) | Spent mushroom substrate microbial milk-cow feed and preparation method thereof | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN103289937B (en) | Method for producing bacillus laterosporus live bacteria by high density solid fermentation | |
| CN103504123A (en) | Fermented soybean meal with function of complex enzymes and preparation method for fermented soybean meal | |
| CN107801837A (en) | A kind of fodder utilizes the bio-fermentation process of chicken manure | |
| CN103098981A (en) | Dewatered solid combined inoculant for increasing protein level of citrus pulp feed and preparation method of dewatered solid combined inoculant | |
| CN101214006B (en) | Method for using EM original liquid fermentation apple pomace to produce protein feed | |
| CN109971682A (en) | A kind of EM bacterium solution for aquaculture and preparation method thereof and application | |
| CN106306361A (en) | Method for preparing biological fermentation feed | |
| CN101280272A (en) | Microbial preparation, preparation and use thereof | |
| CN104855674A (en) | Production method for microbial fermentation complete feed by combining strain joint transformations | |
| CN107779414A (en) | A kind of manioc waste fermentative microorganism microbial inoculum | |
| CN103451132A (en) | Composite microecological preparation used for fermentation of breeding feeds | |
| CN107173556A (en) | A kind of processing method for making an addition to manioc waste in sheep feed | |
| CN102669409B (en) | Method for preparing fermentation promoting peptide of fermented feed from mushroom residue | |
| CN103074228A (en) | Composite fermentation inoculant for processing animal wastes, and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |