CN105891180A - Novel full-automatic time resolution fluorescence analysis device and use method thereof - Google Patents
Novel full-automatic time resolution fluorescence analysis device and use method thereof Download PDFInfo
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- CN105891180A CN105891180A CN201610416029.7A CN201610416029A CN105891180A CN 105891180 A CN105891180 A CN 105891180A CN 201610416029 A CN201610416029 A CN 201610416029A CN 105891180 A CN105891180 A CN 105891180A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract
The invention discloses a novel full-automatic time resolution fluorescence analysis device which comprises an X-axis unit (1), a sample injecting unit (2), a sample putting unit (3), a waste tip recovering unit (4), a frame unit (5), a barrel unit (6), an elevator type transport unit (7), a tower type plate washing unit (8), an independent vibration incubation unit (9), a rotary platform unit (10) and a time resolution fluorescence detecting unit (11). The tower type plate washing unit (8), the independent vibration incubation unit (9), the rotary platform unit (10) and the time resolution fluorescence detecting unit (11) are sequentially arranged on the right side of the frame unit (5) from top to bottom relative to the elevator type transport unit (7). The novel full-automatic time resolution fluorescence analysis device is completely designed for the TRFIA, the time resolution fluorescence analysis process can be rapidly and accurately completed, detection efficiency is improved, and labor cost is reduced.
Description
Technical field
The invention belongs to medical detection technology, particularly relate to a kind of new automatic Time-resolved fluorescence assay
Device and using method thereof.
Background technology
(Time-resolved Fluorescence Immunoassay is called for short time resolved fluoro-immunoassay
TRFIA) it is when former is widely used in the important immune analysis method on clinical and scientific research, and it utilizes group of the lanthanides
Rubidium marking antigen or antibody, according to the luminous characteristics of lanthanide chelate, measure glimmering by TIME RESOLVED TECHNIQUE
Light, detection wavelength and two parameters of time carry out signal resolution simultaneously, can effectively get rid of non-specific fluorescence
Interference, is greatly enhanced the sensitivity of analysis.Therefore, the method have highly sensitive, background values is low, can weigh
The features such as repetition measurement amount.
But, there is presently no the professional automatic equipment for TRFIA Technology design.The most common
Way be to select general blood treatment platform (FreedomEVO, HAMILTON such as Tecan company
The ELISA-STAR etc. of company) go to realize the automatization of TRFIA experiment.
Additionally, patent CN104713858A discloses a kind of full-automatic Time-resolved fluorescence assay instrument apparatus and makes
By method, although close to apparatus of the present invention in technology is applied, but the mechanism part of whole device, mechanism
Design, the process of realization have the biggest difference；Owing to previous platform is comprehensive relatively strong, subject range is relatively wide, the most specially
Door designs for TRFIA, and when carrying out TRFIA experiment hence with these platforms, some functional unit is with actual
Application has part variation, causes functional unit utilization rate too low, causes installation cost to waste.
Summary of the invention
The present invention solves the problems referred to above of the prior art, propose a kind of new automatic time-resolved fluorescence and divide
Analysis apparatus and using method thereof, can analyze process by deadline resolved fluorometric rapidly and accurately, improve detection effect
Rate, reduces human cost.
For achieving the above object, the present invention is by the following technical solutions:
The first aspect of the invention is to provide a kind of new automatic Time-resolved fluorescence assay device, including X
Axle unit 1, sample filling unit 2, sample placement unit 3, useless tip head recovery unit 4, frame unit 5,
Bucket unit 6, Elevator handling unit 7, tower wash Slab element 8, unit 9, rotation platform are hatched in independent concussion
Unit 10, time-resolved fluorescence detector unit 11；Wherein,
Described X-axis unit 1, is arranged on frame unit 5, is used for carrying sample filling unit 2 at its unit
Upper axially-movable；
Described sample filling unit 2, is used for being responsible for sample preparation, preparation of reagents, and shifts sample, reagent extremely
Independent concussion is hatched in the micropore cup on unit 9；
Described sample placement unit 3, including the sample rest area set gradually, calibration object rest area, consumptive material tip
Head rest area；
Described useless tip head recovery unit 4, the useless tip head after collection instrument uses；
Described bucket unit 6, for for and tower wash liquid required when Slab element 8 works, be responsible for receipts simultaneously
Collect tower and wash waste liquid produced by Slab element 8；
Described Elevator handling unit 7, is arranged on the right side of frame unit 5, and the grasping plate unit in unit can be
Movement in vertical direction, for by microwell plate time-resolved fluorescence detector unit 11, independent concussion hatch unit 9,
Tower washing is moved between Slab element 8；
Unit 9 is hatched in described independent concussion, for several and uniform with on described rotation platform unit 10, have
Independent control function, the sample in microwell plate carries out concussion and hatches, and fills platform feature simultaneously as sample；
Described rotation platform unit 10, the independence concussion being mainly used in changing several hatches unit 9 in each merit
The switching of energy position；Preferably, unit 9 is hatched in described independent concussion is 8；
Described time-resolved fluorescence detector unit 11, for carrying out fluoroscopic examination to the sample in microwell plate.
Further, also include control unit, described control unit and X-axis unit 1, sample filling unit 2,
Elevator handling unit 7, tower wash Slab element 8, unit 9, rotation platform unit 10 and are hatched in independent concussion
Time-resolved fluorescence detector unit 11 is electrically connected with.
Further, described sample filling unit 2 has the barcode scanner of identification agent；Described microwell plate has
There is different identification bar codes；Described Elevator handling unit 7 has barcode scanner.
Further, described tower wash Slab element 8, unit 9, rotation platform unit 10 and are hatched in independent concussion
Time-resolved fluorescence detector unit 11 is sequentially arranged in frame unit relative to Elevator handling unit 7 from top to bottom
The right side of 5.
Further, described Elevator handling unit 7, with bracing frame 20 as agent structure, passes through stepper drive
15, drive mechanism 16, guide rail 17 make grasping plate unit 21 do Z-direction motion；Described grasping plate unit 21 leads to
Over-drive unit 18, drive mechanism 19 are X to motion.
Further, described tower wash Slab element 8 mainly by fluid injection shower nozzle 24 and, Z-direction motion 22,
Transverse movement guide rail 23 forms；Liquid in described bucket unit 6 is interior by pipeline 27, control valve 26 is controlled
Tubbing unit 6 and fluid injection shower nozzle 24 inject in micropore cup 25.
Further, described rotation platform unit 10 is evenly distributed with several and hatches list for independent concussion
The placement liquid carrier 14 of unit 9, described rotation platform unit 10 is by servo-drive 12, synchronous belt mechanism
Ring rotation motion is done in 13 drives.
Further, described independent concussion is hatched and is had temperature controller and heater in unit 9.
The second aspect of the invention is to provide the use of a kind of new automatic Time-resolved fluorescence assay device
Method, comprises the following steps:
Step 1, sample is placed on sample placement unit 3, microwell plate is positioned over independent concussion and hatches on unit 9,
Reagent class container such as micropore cup is positioned in the microwell plate of correspondence；
The sample of sample rest area 3 is filled by step 2, sample filling unit 2 hatches on unit 9 in independent concussion
In microwell plate, after often filling once, sample filling unit 3 all repeats to take tip for the first time, to avoid polluting；
Step 3, microwell plate are hatched room temperature concussion in unit 9 in independent concussion and are hatched；
Step 4, sample filling unit 2 presses reagent configuration needs, and reagent preparation is in diluted cup；
Independent concussion is hatched unit 9 and is rotated to Elevator handling unit 7 corresponding by step 5, rotation platform unit 10
Position, Elevator handling unit 7 be carried to microwell plate tower to wash Slab element 8；
Microwell plate is cleaned by step 6, tower Slab element 8 of washing several times；Fluid injection shower nozzle 24 is by microwell plate
Micropore fills cleaning mixture, soaks about one second kind, then cleaning mixture is siphoned away, be and once clean；
Microwell plate is carried to independently shake hatch unit 9 by step 7, Elevator handling unit 7；
Good reagent configured in step 4 is filled by step 8, sample filling unit 2 hatches unit 9 in independent concussion
On microwell plate in, microwell plate is hatched concussion in unit 9 in independent concussion and is hatched clock；
Step 9, repeat step 5,6；
Microwell plate is carried to tower wash Slab element 8 by step 10, Elevator handling unit 7, from fluid injection shower nozzle 24 toward
In microwell plate, filling strengthens liquid；
Microwell plate is carried to independently shake hatch unit 9 by step 11, Elevator handling unit 7, and microwell plate is in independence
Concussion is hatched concussion in unit 9 and is hatched；
Microwell plate is hatched unit 9 from independent concussion by step 12, Elevator handling unit 7, and to be carried to time resolution glimmering
In optical detecting unit 11, read fluorescent value；
Microwell plate is moved to concussion from time-resolved fluorescence detector unit 11 and incubates by step 13, Elevator handling unit 7
Educate unit 9, removed after experiment terminates.
Further, in step 6 and step 9, the described fluid injection shower nozzle 24 micropore on microwell plate is noted
Full cleaning mixture, siphons away cleaning mixture after immersion again, completes once to clean.
The present invention uses technique scheme, compared with prior art, has the following technical effect that
The new automatic Time-resolved fluorescence assay device of the present invention is directed entirely to TRFIA design, closes
Removing the work puts structure and the position of each functional module, and is realized the scheduling controlling of different experiments step by control unit
And optimum organization, process can be analyzed by deadline resolved fluorometric rapidly and accurately, improve detection efficiency, reduce
Human cost.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the present invention new automatic Time-resolved fluorescence assay device；
Fig. 2 is the structural representation of rotation platform unit in analytical equipment of the present invention；
Fig. 3 is the structural representation of Elevator handling unit in analytical equipment of the present invention；
Fig. 4 is the tower structural representation washing Slab element in analytical equipment of the present invention；
Wherein, 1-X axle unit, 2-sample filling unit, 3-sample placement unit, 4-gives up tip head recovery unit, 5-
Frame unit, 6-bucket unit, 7-Elevator handling unit, the tower Slab element of washing of 8-, 9-independently shakes hatches list
Unit, 10-rotation platform unit, 11-time-resolved fluorescence detector unit, 12-servo-drive, 13-Timing Belt machine
Structure, 14-liquid carrier, 15-stepper drive, 16-drive mechanism, 17-guide rail, 18-driver element, 19-
Drive mechanism, 20-bracing frame, 21-grasping plate unit, 22-Z is to motion, 23-transverse movement guide rail, 24-
Fluid injection shower nozzle, 25-micropore cup.
Detailed description of the invention
Below by specific embodiment, the present invention is carried out detailed and concrete introduction, so that being better understood from this
Bright, but following embodiment is not limiting as the scope of the invention.
As it is shown in figure 1, present embodiments provide a kind of new automatic Time-resolved fluorescence assay device, including
X-axis unit 1, sample filling unit 2, sample placement unit 3, useless tip head recovery unit 4, frame unit
5, bucket unit 6, Elevator handling unit 7, tower wash Slab element 8, independent concussion is hatched unit 9, is rotated
Platform unit 10, time-resolved fluorescence detector unit 11；Wherein,
Described X-axis unit 1, is arranged on frame unit 5, is used for carrying sample filling unit 2 at its unit
Upper axially-movable；
Described sample filling unit 2, is used for being responsible for sample preparation, preparation of reagents, and shifts sample, reagent extremely
Independent concussion is hatched in the micropore cup on unit 9；
Described sample placement unit 3, including the sample rest area set gradually, calibration object rest area, consumptive material tip
Head rest area；
Described useless tip head recovery unit 4, the useless tip head after collection instrument uses；
Described bucket unit 6, for for and tower wash liquid required when Slab element 8 works, be responsible for receipts simultaneously
Collect tower and wash waste liquid produced by Slab element 8；
Described Elevator handling unit 7, is arranged on the right side of frame unit 5, and the grasping plate assembly in unit can be
Movement in vertical direction, for by microwell plate time-resolved fluorescence detector unit 11, independent concussion hatch unit 9,
Tower washing is moved between Slab element 8；
Unit 9 is hatched in described independent concussion, has independent control function, and the sample in microwell plate shakes
Hatch, fill platform feature simultaneously as sample；
Described rotation platform unit 10, is mainly used in changing 8 independent concussions and hatches unit 9 at each functional position
The switching put；
Described time-resolved fluorescence detector unit 11, for carrying out fluoroscopic examination to the sample in microwell plate.
As a preferred embodiment, also include control unit, described control unit and X-axis unit 1, sample
This filling unit 2, Elevator handling unit 7, tower wash Slab element 8, independent concussion is hatched unit 9, is rotated
Platform unit 10 and time-resolved fluorescence detector unit 11 are electrically connected with.
As a preferred embodiment, described sample filling unit 2 has the barcode scanner of identification agent；
Described microwell plate has different identification bar codes；Described Elevator handling unit 7 has barcode scanner.
As a preferred embodiment, described tower wash Slab element 8, independent concussion is hatched unit 9, rotated
Platform unit 10 and time-resolved fluorescence detector unit 11 are from top to bottom relative to Elevator handling unit 7 successively
It is located at the right side of frame unit 5.
As it is shown on figure 3, described Elevator handling unit 7 is with bracing frame 20 as agent structure, driven by stepping
Dynamic 15, drive mechanism 16, guide rail 17 make grasping plate unit 21 do Z-direction motion；Described grasping plate unit 21
It is X to motion by driver element 18, drive mechanism 19.
As shown in Figure 4, described tower wash Slab element 8 mainly by fluid injection shower nozzle 24 and, Z-direction motion 22,
Transverse movement guide rail 23 forms；Liquid in described bucket unit 6 is interior by pipeline 27, control valve 26 is controlled
Tubbing unit 6 and fluid injection shower nozzle 24 inject in micropore cup 25.
Hatch as in figure 2 it is shown, be evenly distributed with several on described rotation platform unit 10 for independent concussion
The placement liquid carrier 14 of unit 9, described rotation platform unit 10 is by servo-drive 12, synchronous belt mechanism
Ring rotation motion is done in 13 drives.
Preferably, described independent concussion is hatched and is had temperature controller and heater in unit 9.
The TRFIA experiment of Application Example hepatitis B surface antigen (HBsAg)
Step 1, preparation, be placed in sample placement unit 3 by sample serum or blood plasma；By HBsAg
The microwell plate of project is positioned over independent concussion and hatches on unit 9, is positioned over by the reagent class container of this project simultaneously
Corresponding region；Generally use 96 hole microwell plates, i.e. every plate can do 96 tests according to industrial practice, if any
Multiple projects, multiple microwell plates are loaded into independent concussion successively and hatch on unit 9, at most can load 8；
The sample of sample rest area 3 is filled by step 2, sample filling unit 2 hatches unit 9 in independent concussion
On microwell plate in, every hole 100 μ L；After often filling once, it is first that sample filling unit 3 all repeats to take tip
Secondary, to avoid polluting；Certainly, sample empirically demand percentage also can be diluted by sample filling unit 3, this reality
The HBsAg project sample executing example need not dilution；
Step 3, HBsAg microwell plate are hatched room temperature concussion in unit 9 in independent concussion and are hatched 40 minutes；By
Hatch unit 9 in independent concussion and there is temperature controller and heater, as project needs, can the process hatched of earthquake
In heat；
Step 4, sample filling unit 2 presses reagent configuration needs, and reagent preparation is in diluted cup；
The independence of HBsAg is shaken by step 5, rotation platform unit 10 hatches unit 9, rotates to Elevator
The position of handling unit 7 correspondence；HBsAg microwell plate is carried to tower wash plate list by Elevator handling unit 7
In unit 8；
HBsAg microwell plate is cleaned 4 times by step 6, tower Slab element 8 of washing, and washes wrench by microwell plate
Micropore fills cleaning mixture, soaks about one second kind, then cleaning mixture is siphoned away, be and once clean；In this enforcement
Example is provided with two and washes Slab element, during to solve the experiment doing multiple project, wash asking of Slab element inadequate resource
Topic；
HBsAg microwell plate is carried to independently shake by step 7, Elevator handling unit 7 hatches unit 9；
Good reagent configured in step 5 is filled by step 8, sample filling unit 2 hatches list in independent concussion
In microwell plate in unit 9, microwell plate is hatched concussion in unit 9 in independent concussion and is hatched 40 minutes；
Step 9, the step 6 that repeats, 7,8, but wash plate number of times and change 6 times into；
HBsAg microwell plate is carried to tower wash Slab element 8 the superiors by step 10, Elevator handling unit 7
Strengthening liquid filling assembly, liquid feeding head strengthens the special agent in liquid time resolution test kit toward filling in microwell plate,
Every hole 200 μ L；
HBsAg microwell plate is carried to independently shake by step 11, Elevator handling unit 7 hatches unit 9；
Microwell plate is hatched concussion in unit 9 in independent concussion and is hatched 5 minutes；
HBsAg microwell plate is hatched unit 9 from independent concussion and is carried to by step 12, Elevator handling unit 7
In time-resolved fluorescence detector unit 11, read fluorescent value；
Step 13, Elevator handling unit 7 by HBsAg microwell plate from time-resolved fluorescence detector unit 11
In be carried to independently shake and hatch unit 9, removed after experiment terminates.
Above-mentioned only explanation as a example by the TRFIA experiment of hepatitis B surface antigen (HBsAg), carries out other TRFIA
During experiment, wash plate number of times, the concussion type such as incubation time and temperature, reagent and enhancing liquid can convert accordingly.
The structure of each functional module of reasonable Arrangement of the present invention and position, and realize different experiments step by control unit
Rapid scheduling controlling and optimum organization, automatically can complete test experiments to fast and reliable, improve testing efficiency,
Decrease artificial participation.
Being described in detail the specific embodiment of the present invention above, but it is intended only as example, the present invention is also
It is not restricted to particular embodiments described above.To those skilled in the art, any the present invention is carried out
Equivalent modifications and substitute the most all among scope of the invention.Therefore, without departing from the spirit of the present invention and model
Enclose lower made impartial conversion and amendment, all should contain within the scope of the invention.
Claims (10)
1. a new automatic Time-resolved fluorescence assay device, it is characterised in that include X-axis unit (1),
Sample filling unit (2), sample placement unit (3), useless tip head recovery unit (4), frame unit (5),
Bucket unit (6), Elevator handling unit (7), tower wash Slab element (8), unit is hatched in independent concussion
(9), rotation platform unit (10), time-resolved fluorescence detector unit (11)；Wherein,
Described X-axis unit (1), is arranged on frame unit (5), is used for carrying sample filling unit (2)
In the upper axially-movable of X-axis unit (1)；
Described sample filling unit (2), is arranged on X-axis unit (1), is used for being responsible for sample preparation, examination
Agent is prepared, and shift sample, reagent is hatched in the micropore cup on unit (9) to independent concussion；
Described sample placement unit (3), including the sample rest area set gradually, calibration object rest area, consumptive material
Tip head rest area；
Described Elevator handling unit (7), is arranged on the right side of frame unit (5), and described Elevator is carried
Grasping plate unit (21) in unit (7) can be in movement in vertical direction, for microwell plate is glimmering in time resolution
Optical detecting unit (11), unit (9) is hatched in independent concussion, tower washing is moved between Slab element (8)；
Unit (9) is hatched in described independent concussion, has independent control function, and the sample in microwell plate is carried out
Concussion is hatched, and fills platform simultaneously as sample；
Described rotation platform unit (10), is mainly used in changing several independence concussion and hatches unit (9) and exist
The switching of each POF；
Described time-resolved fluorescence detector unit (11), for carrying out fluoroscopic examination to the sample in microwell plate.
New automatic Time-resolved fluorescence assay device the most according to claim 1, it is characterised in that also
Including control unit, described control unit and X-axis unit (1), sample filling unit (2), Elevator
Handling unit (7), tower wash Slab element (8), unit (9), rotation platform unit are hatched in independent concussion
(10) it is electrically connected with time-resolved fluorescence detector unit (11).
New automatic Time-resolved fluorescence assay device the most according to claim 1, it is characterised in that institute
State sample filling unit (2) and there is the barcode scanner of identification agent；Described microwell plate has different identification
Bar code；Described Elevator handling unit (7) has barcode scanner.
New automatic Time-resolved fluorescence assay device the most according to claim 1, it is characterised in that institute
State and tower wash Slab element (8), independent concussion is hatched unit (9), rotation platform unit (10) and time and is divided
Distinguish that fluorescence detection unit (11) is sequentially arranged in frame unit relative to Elevator handling unit (7) from top to bottom
(5) right side.
New automatic Time-resolved fluorescence assay device the most according to claim 1, it is characterised in that institute
State Elevator handling unit (7) with bracing frame (20) as agent structure, by stepper drive (15), pass
Motivation structure (16), guide rail (17) make grasping plate unit (21) do Z-direction motion；Described grasping plate unit (21)
It is X to motion by driver element (18), drive mechanism (19).
New automatic Time-resolved fluorescence assay device the most according to claim 1, it is characterised in that institute
State tower wash Slab element (8) mainly by fluid injection shower nozzle (24) and, Z-direction motion (22), laterally transport
Dynamic guide rail (23) composition；Liquid in described bucket unit (6) is interior by pipeline (27), control valve (26)
Control a barrel unit (6) and fluid injection shower nozzle (24) injects in micropore cup (25).
New automatic Time-resolved fluorescence assay device the most according to claim 1, it is characterised in that institute
State and on rotation platform unit (10), be evenly distributed with several placements hatching unit (9) for independent concussion
Liquid carrier (14), described rotation platform unit (10) passes through servo-drive (12), synchronous belt mechanism (13)
Ring rotation motion is done in drive.
New automatic Time-resolved fluorescence assay device the most according to claim 1, it is characterised in that institute
State described independent concussion to hatch in unit (9) there is temperature controller and heater.
The using method of new automatic Time-resolved fluorescence assay device the most as claimed in claim 1, its feature
It is, comprises the following steps:
Step 1, sample is placed on sample placement unit (3), microwell plate is positioned over independent concussion and hatches unit (9)
On, reagent class container is positioned in the microwell plate of correspondence；
The sample of sample rest area (3) is filled by step 2, sample filling unit (2) hatches unit in independent concussion
(9), in the microwell plate on, after often filling once, sample filling unit 3 all repeats to take tip for the first time, to keep away
Exempt to pollute；
Step 3, microwell plate are hatched room temperature concussion in unit (9) in independent concussion and are hatched；
Reagent configuration needs pressed by step 4, sample filling unit (2), and reagent preparation is in diluted cup；
Independent concussion is hatched unit (9) and is rotated to Elevator handling unit by step 5, rotation platform unit (10)
(7) corresponding position, is carried to microwell plate tower to wash Slab element (8) by Elevator handling unit (7)
In；
Microwell plate is cleaned by step 6, tower Slab element (8) of washing several times；Fluid injection shower nozzle (24) is by micropore
Micropore on plate fills cleaning mixture, soaks about one second kind, then cleaning mixture is siphoned away, be and once clean；
Microwell plate is carried to independently shake by step 7, Elevator handling unit (7) hatches unit (9)；
Good reagent configured in step 4 is filled by step 8, sample filling unit (2) hatches list in independent concussion
In microwell plate in unit (9), microwell plate is hatched concussion in unit (9) in independent concussion and is hatched clock；
Step 9, repetition step 5, step 6；
Microwell plate is carried to tower wash Slab element (8) by step 10, Elevator handling unit (7), fluid injection spray
Head (24) strengthens liquid toward filling in microwell plate；
Microwell plate is carried to independently shake by step 11, Elevator handling unit (7) hatches unit (9), micropore
Plate is hatched concussion in unit (9) in independent concussion and is hatched；
Microwell plate is hatched unit (9) from independent concussion and is carried to the time by step 12, Elevator handling unit (7)
In resolved fluorometric detector unit (11), read fluorescent value；
Microwell plate is moved to from time-resolved fluorescence detector unit (11) by step 13, Elevator handling unit (7)
Unit (9) is hatched in concussion, removed after experiment terminates.
New automatic Time-resolved fluorescence assay device the most according to claim 1, it is characterised in that
In step 6 and step 9, described fluid injection shower nozzle (24) fills cleaning mixture in the micropore on microwell plate, soaks
After again cleaning mixture is siphoned away, complete once to clean.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110567949A (en) * | 2019-09-10 | 2019-12-13 | 广东天地和实业控股集团有限公司 | bacterial toxin detection method and treatment instrument applied to same |
| CN113156101A (en) * | 2021-04-08 | 2021-07-23 | 中国科学院苏州生物医学工程技术研究所 | Multi-wavelength time-resolved fluorescence immunoassay analyzer |
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| CN110567949A (en) * | 2019-09-10 | 2019-12-13 | 广东天地和实业控股集团有限公司 | bacterial toxin detection method and treatment instrument applied to same |
| CN110567949B (en) * | 2019-09-10 | 2021-12-14 | 广东天地和实业控股集团有限公司 | Bacterial toxin detection method and treatment instrument applied to same |
| CN113156101A (en) * | 2021-04-08 | 2021-07-23 | 中国科学院苏州生物医学工程技术研究所 | Multi-wavelength time-resolved fluorescence immunoassay analyzer |
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Application publication date: 20160824 |