CN113156101A - Multi-wavelength time-resolved fluorescence immunoassay analyzer - Google Patents
Multi-wavelength time-resolved fluorescence immunoassay analyzer Download PDFInfo
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- CN113156101A CN113156101A CN202110378520.6A CN202110378520A CN113156101A CN 113156101 A CN113156101 A CN 113156101A CN 202110378520 A CN202110378520 A CN 202110378520A CN 113156101 A CN113156101 A CN 113156101A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
The invention discloses a multi-wavelength time-resolved fluorescence immunoassay analyzer, which comprises an instrument rack system, a sample system, a reagent system, a cup feeding system, a filling system, a detection system, a waste storage system and an incubation system, wherein the sample system, the reagent system, the cup feeding system, the filling system, the detection system, the waste storage system and the incubation system are arranged in the instrument rack system; accomplish the sample transportation through sample frame bracket, drag the transportation that sends the hand and accomplish analysis glass stand through bar analysis glass stand, accomplish the transportation of reagent through reagent frame tray, accomplish the filling of sample, reagent 1, reagent 2 through the filling system, mix the even back through hatching, detect the sample, detected the wastes material and stored to waste storage system, whole instrument compact structure, good reliability, detection flux is big, detects the precision height.
Description
Technical Field
The invention belongs to the technical field of in-vitro diagnosis medical instruments, and particularly relates to a multi-wavelength time-resolved fluorescence immunoassay analyzer.
Background
For more than half a century, the mainstream immunoassay technology is radio immunoassay, enzyme-linked immunoassay, chemiluminescence immunoassay and the like, wherein the radio immunoassay is used in a small amount at present due to the problem of radioactivity, the enzyme-linked immunoassay is gradually replaced due to long detection time, and the chemiluminescence immunoassay is the mainstream immunoassay technology at present. In the field of instant detection such as emergency treatment and the like, chemiluminescence is difficult to widely apply due to complex technology and high cost, and the demands promote instant detection type immunoassay technologies such as colloidal gold immunoassay, fluorescence chromatography immunoassay, colloidal turbidimetric immunoassay and the like. The chemiluminescence immunoassay technology is born in the seventies of the last century, the main technology adopted is magnetic particle chemiluminescence, the core hypothesis is that the separation of specific immune complexes and biological matrixes can be realized certainly through magnetic separation, but in clinical practical application, due to the diversity of clinical specimens, cases of magnetic separation failure cannot be avoided, such as 'jump value' phenomenon caused by nonspecific adsorption due to fibrin adhesion, false positive caused by heterophilic antibodies and the like. Half of the failures are due to magnetic cleaning failures for the instrument itself. Therefore, the cleaning-free immunoassay technology is a research hotspot for nearly two-thirty years, siemens, beckmann and other companies have related technology reserves, a series of high-flux immunoassay systems are provided by Beijing Koimei corporation of domestic enterprises based on the Siemens patent light-activated chemiluminescence technology, and the clinical application of cleaning-free detection is realized for the first time.
The time-resolved fluorescence resonance energy transfer immunoassay (TR-FRET) technology was originally developed by a supramolecular rare earth cryptand ether probe discovered by German Bolames (brahms) company based on Jean-Marie Lehn, Nobel chemical prize-winning France, and overcomes the influence of abnormal alignment accuracy of samples such as hemolysis, hyperlipidemia, jaundice and the like in homogeneous detection. Compared with other immunoassay technologies, the technology has many advantages, does not need a solid phase carrier and a solid phase probe, and has the advantages of good precision and accuracy, simple operation, easy automation and miniaturization and the like.
The core of the time-resolved fluorescence resonance energy transfer method is that two fluorescence molecules are called donor and acceptor respectively, wherein the donor can absorb exciting light and emit fluorescence, and the fluorescence can also be transmitted to the acceptor in a fluorescence resonance energy transfer mode and emitted by the acceptor. Specifically, a chelate label of a rare earth element having a cryptic structure is used as a fluorescence donor, and a short-lived fluorescent molecule having a good spectral overlap with the fluorescence donor is used as a fluorescence acceptor, and Fluorescence Resonance Energy Transfer (FRET) occurs between the donor and the acceptor (second fluorescent label) of the cryptic compound of a rare earth element. In fluorescence resonance energy transfer, the lifetime of the acceptor emitted fluorescence approaches that of the donor. Because the donor fluorescence decay period is long, the donor induces the acceptor to emit fluorescence for a long time, and the fluorescence generated after the acceptor is excited can last for a long time, so that the self-scattered fluorescence with short lifetime can be distinguished through time resolution, and the FRET signal can be easily distinguished from the background of the fluorescence with short lifetime.
The fluorescent donor and acceptor can be covalently linked to different partner molecules, e.g., protein dimers, complementary strands of DNA, antigens and antibodies, ligands and acceptors, and the like. Conventional FRET fluorescent compounds are susceptible to interference from background fluorescence of the sample (serum, plasma, buffers, proteins, chemicals and cell lysates). This background fluorescence has a very short lifetime (on the order of 10-9 seconds) and is easily removed by time-resolved methods. The time-resolved fluorescence resonance energy transfer (TR-FRET) technique combines the FRET technique and the time-resolved fluorescence measurement, and removes the extremely short-lived background fluorescence. After transient photoexcitation, the non-specific short-lived emission drops to zero after a delay of 50-150 microseconds. Whereas TR-FRET fluorophores emit long-lived fluorescence that participates in the FRET process. Thus, long-lived acceptor emission represents the energy transfer upon molecular binding.
Disclosure of Invention
The invention aims to provide a multi-wavelength time-resolved fluorescence immunoassay analyzer to improve the accuracy of immunoassay.
The technical solution for realizing the purpose of the invention is as follows:
a multi-wavelength time-resolved fluorescence immunoassay analyzer comprises an instrument rack system, a sample system, a reagent system, a cup feeding system, a filling system, a detection system, a waste storage system and an incubation system, wherein the sample system, the reagent system, the cup feeding system, the filling system, the detection system, the waste storage system and the incubation system are arranged in the instrument rack system;
the sample system is provided with a first placing area of a plurality of sample racks and is used for conveying the sample racks corresponding to the first placing area to the collecting area;
the reagent system is provided with a second placing area of the reagent rack and is used for conveying the reagent rack in the second placing area to the collecting area;
the filling system comprises a sample filling module and a reagent filling module, and the sample filling module and the reagent filling module are respectively used for filling a sample and a reagent corresponding to the collection area into an analysis cup on an analysis cup holder of the cup feeding system;
the cup feeding system is provided with a plurality of placing positions of the analyzing cup holders and is used for conveying the analyzing cup holders corresponding to the placing positions to the incubation system;
the incubation system is provided with a reciprocating module and a heating module which are respectively used for mixing and heating a reagent and a sample to complete incubation; after the incubation is finished, the cup feeding system conveys the analysis cup holder in the incubation system to the detection system;
the detection system provides a detection light source for completing the acquisition of photon signals; after the detection is complete, the cup transport system transports the analyzing cup holders within the detection system to a waste storage system.
Compared with the prior art, the invention has the following remarkable advantages:
the device has the advantages of compact overall structure design, high automation degree, high detection efficiency, good reliability and high detection precision.
Drawings
Fig. 1 is an exploded view of the complete machine assembly of the present invention.
FIG. 2 is a schematic view of the instrument rack system of the present invention.
FIG. 3 is a schematic diagram of a sample system of the present invention.
FIG. 4 is a schematic diagram of a reagent system of the present invention.
Fig. 5 is a schematic view of a filling module of the filling system of the present invention.
FIG. 6 is a schematic view of the cup feed system of the present invention.
FIG. 7 is a schematic view of the detection system of the present invention.
Figure 8 is a schematic view of the waste storage system of the present invention.
FIG. 9 is a schematic of an incubation system of the present invention.
Detailed Description
The invention is further described with reference to the following figures and embodiments.
Referring to fig. 1, the multi-wavelength time-resolved fluorescence immunoassay analyzer of the present invention includes an apparatus rack system 1, a sample system 2, a reagent system 3, a cup feeding system 4, a filling system 5, a detection system 6, a waste storage system 7, and an incubation system 8. The sample system 2 is installed on the instrument bottom plate 102, the reagent system 3 is installed on the left side area of the instrument bottom plate 102, the cup feeding system 4 is installed on the front side of the middle area of the instrument middle base plate 103, the filling system 5 is installed on the left side cross beam of the main body frame 101, the detection system 6 is installed at the upper left corner of the instrument middle base plate 103, and the incubation system 8 is installed on the rear side of the middle area of the instrument middle base plate 103, so that the whole multi-wavelength time-resolved fluorescence immunoassay analyzer is formed.
Referring to fig. 2, the instrument rack system 1 is supported by an instrument main frame 101, the main frame 101 is a sheet metal part, rigidity is guaranteed, and overall mass is effectively reduced, an instrument bottom plate 102 is installed at the bottom in the main frame 101 through bolt connection, an instrument middle base plate 103 is connected through a connecting piece, and is fixed through bolts, installed at the middle height position of the main frame 101, and a liquid path element storage area 104 is connected through a connecting piece, and is fixed through bolts, installed on the left side of the main frame 101, and used for installing a liquid path pump valve. The electric control system mounting section 105 is mounted at a front position on the left side of the main body frame 101.
Referring to fig. 3, the sample system 2 includes a sample rack spacer 201, a first X-axis transmission mechanism, a first Y-axis transmission mechanism, a sample rack bracket 204, and a first detection unit;
the sample system 2 is formed by fixing more than ten sample rack spacing blocks 201 made of engineering plastics on the instrument bottom plate 102 at intervals according to the width of the sample rack to form a plurality of first placing areas of the sample rack, wherein each first placing area is provided with a photoelectric sensor as a first detection unit and can sense whether the sample rack is placed at a corresponding position. The first X-axis transmission mechanism comprises a first ball guide rail 202 in the X-axis direction, a first ball slide block 203, a sample frame bracket 204, a first rotating motor 205 and a first synchronous belt 206; the first Y-axis transmission mechanism comprises a second rotating motor 208 and a sample rack dragging hand 207; the first ball guide rail 202 is fixed on a groove of the instrument base plate 102 in the X-axis direction through a screw, the first ball slide block 203 is installed on the first ball guide rail 202, the sample frame bracket 204 is fixed on the first ball slide block 203 through a screw connection and is driven by a first rotating motor 205 fixed on the instrument base plate 102, the sample frame bracket 204 is driven to move in the X-axis direction through a first synchronous belt 206, a sample frame dragging hand 207 is installed above the sample frame bracket 204 and is driven by a second rotating motor 208 fixed on the first ball slide block 203, the sample frame dragging hand 207 is rotated clockwise by the second rotating motor 208 and stretches out, and the sample frame dragging hand 207 is rotated anticlockwise by the second rotating motor 208 and retracts. After a sample is placed in the sample rack, the sample and the sample rack are placed in a sample rack placing area formed by the sample rack partition blocks 201, and after the system senses the sample rack placed at the corresponding digital position, the sample rack is dragged to the sample rack bracket 204 from the sample rack placing area by the sample rack dragging hand 207 and moves along the Y-axis direction.
As shown in fig. 4, the reagent system 3 is provided with a second Y-axis transport mechanism and a second detection unit, the second Y-axis transport mechanism includes a second ball guide 301, a second ball slider 302, a third rotating motor 304, and a second synchronous belt 305, which are arranged along the Y-axis direction; the second ball guide rail 301 is fixed on a groove in the left Y-axis direction of the instrument bottom plate 102 through screws, the second ball slide block 302 is installed on the second ball guide rail 301, the reagent rack tray 303 is installed on the second ball slide block 302 through screw connection and is driven by a third rotating motor 304, a second synchronous belt 305 drives the reagent rack tray 303 to move along the Y-axis direction in a transmission mode, a code bar 306 is installed at the side end of the reagent rack tray 303 through screw connection, three photoelectric sensors 307, 308 and 309 are installed at the side end of the second ball guide rail 301 and serve as a second detection unit, and different suction positions can be determined in the moving process of the reagent rack tray 303 along the Y axis through the cooperation of the code bar 306 and the three photoelectric sensors 307, 308 and 309.
As shown in fig. 5, the filling system 5 is composed of three filling modules arranged side by side, which are respectively a sample filling module, a first reagent filling module and a second reagent filling module, and the three filling modules have the same structure and respectively include a second X-axis transmission mechanism, a Z-axis transmission mechanism, a third detection mechanism and a filling needle module;
the second X-axis transmission mechanism comprises a base 501, a third ball guide rail 503, a third ball slider 504, a Z-axis substrate 505, a fourth rotating motor 506 and a trapezoidal screw 507; the Z-axis transmission mechanism comprises a fifth rotating motor 511, a third synchronous belt 512, a fourth ball guide rail 508 and a fourth ball slide 509;
the base 501 is fixed on a groove of the instrument middle base plate 103 through bolts, a code bar 502 is arranged on the rear side, a third ball guide rail 503 arranged in the X-axis direction is installed on the groove of the sample filling module base 501 through screws, a third ball slide block 504 is installed on the third ball guide rail 503, a Z-axis base plate 505 is installed on the third ball slide block 504 through screws and driven by a fourth rotating motor 506, a trapezoidal screw 507 is driven to move along the X-axis direction, a photoelectric sensor 513 is installed below the Z-axis base plate 505, the moving position in the X-axis direction is determined by using the code bar 502 (a third detection mechanism) as a grating, the fourth ball guide rail 508 is installed on the Z-axis base plate 505 through screw connection, the fourth ball slide block 509 is installed on the fourth ball guide rail 508, a filling needle module 510 composed of a suction needle and a liquid level detector is installed on the ball slide block 509 through screw connection, and driven by a fifth rotating motor 511 fixed on the Z-axis base plate, the third synchronous belt 512 drives to move along the Z-axis direction, sucks a sample in a sample cup or a reagent in a reagent cup, fills the sample into an analysis cup to complete the filling action of the sample, and a photoelectric sensor 514 is arranged above the Z-axis substrate 505 and is used as a zero switch of the filling module 510.
As shown in fig. 6, the cup feeding system 4 includes a third X-axis transmission mechanism and a third Y-axis transmission mechanism; the third X-axis transmission mechanism comprises a bottom plate 401, a fifth ball guide rail 402, a fifth ball slide block 403, a groove-shaped member 406, a sixth rotating motor 407 and a fourth synchronous belt 408; the third Y-axis transmission mechanism includes a sixth ball guide 410, a sixth ball slider 411, a carrying hand module 412, and a seventh rotating electrical machine 414;
the bottom plate 401 is fixed on a groove of the instrument middle substrate 103 through a screw, 10 baffles are distributed on the upper surface of the bottom plate 401 of the strip-shaped analysis cup holder placement area, corresponding strip-shaped analysis cup holder placement positions are separated according to numbers, each placement position is provided with a photoelectric sensor so as to display whether the strip-shaped analysis cup holder is placed at the position, a fifth ball guide rail 402 arranged in the X-axis direction is installed on the groove of the instrument middle substrate 103 through screw connection, a fifth ball slide block 403 is installed on the fifth ball guide rail 402, a code bar 405 is fixed in front of the fifth ball guide rail 402 through screw connection, a channel-shaped member 406 is installed on the ball slide block 403 through screw connection and driven by a sixth rotating motor 407 fixed on the instrument rack system 1, a fourth synchronous belt 408 is driven to move along the X-axis direction, and an analysis cup holder bracket 409 is fixed on the groove on the upper surface of the lower side of the channel-shaped member 406, a sixth ball guide 410 arranged in the Y-axis direction is mounted in a groove on the lower surface of the upper side of the groove member 406 by screw connection, a sixth ball slider 411 is mounted on the sixth ball guide 410, a dragging and sending hand module 412 is mounted on the sixth ball slider 411, a synchronous belt is driven by a seventh rotating motor 414 to move along the Y-axis direction, so that a strip-shaped analyzing cup holder can be conveyed from the base plate 401 to the analyzing cup holder bracket 409 and then moves along the X-axis along the groove member 406, a photoelectric sensor 415 arranged on the lower surface of the groove member 406 determines the moving position of the groove member 406 along the X-axis direction in cooperation with the code bar 405, and then the dragging and sending hand module 412 can drag the strip-shaped analyzing cup holder in the analyzing cup holder bracket 409 into the incubation system 8 to complete incubation.
With reference to fig. 7, the detection system 6 is composed of a xenon lamp light source 601, a measurement darkroom 602, and a photon counting unit 603, wherein the xenon lamp light source 601 provides a detection light source, the measurement darkroom 602 provides a detection light path and a good dark-avoiding environment, and the photon counting unit 603 collects photon signals excited by the xenon lamp light source in the measurement darkroom.
As shown in fig. 8, the waste storage system 7 includes a fourth Y-axis transport mechanism, a waste storage cassette 704, and the fourth Y-axis transport mechanism includes a waste storage system base plate 701, a seventh ball slide 703, a seventh ball guide 702, a ninth rotating motor 705, and a fifth timing belt 706;
the waste storage system substrate 701 is fixed on the instrument bottom plate 102 through screw connection, a seventh ball guide 702 arranged in the Y-axis direction is fixed at a groove of the waste storage system substrate 701 through screw connection, a seventh ball slider 703 is installed on the seventh ball guide 702, a waste storage box 704 is installed on the seventh ball slider 703 and driven by a ninth rotating motor 705 and a fifth synchronous belt 706 which are arranged on the waste storage system substrate 701, the ninth rotating motor 705 rotates to drive the fifth synchronous belt 706 to drive the waste storage box 704 to move in the Y-axis direction, and the fourth Y-axis transmission mechanism is used for driving the waste storage box 704 to switch from a working position to a pick-and-place position.
As shown in fig. 9, the incubation system 8 includes an incubation disc base 801, an incubation disc upper cover plate 802, a heat insulating layer 803, a heating module 804, a pressing bar 805, a code bar 806, a slider-crank mechanism 807, and an eighth rotating motor 808, where the code bar 806 is used as a grating to determine a position of the channel-shaped member 406 corresponding to the incubation system 8, the incubation disc upper cover plate 802 is fixed on the incubation disc base 801, the heat insulating layer 803 is fixed on the incubation disc upper cover plate 802 through the pressing bar 805, the incubation disc base 801 is provided with a plurality of compartments, the incubation disc base 801, the incubation disc upper cover plate 802, the heat insulating layer 803, and the pressing bar 805 form a heat insulating cavity, the heating module 804 is disposed under the incubation disc base 801 for providing a heat source and controlling temperature, and the eighth rotating motor 808 rotates to drive the slider-crank mechanism 807 to move, thereby driving the incubation disc base 801 to reciprocate along the X-axis direction, and achieving the purpose of uniform mixing. The analyzing cups pulled into the analyzing cup holders in the incubation system 8 are pulled from the incubation system 8 into the analyzing cup holder bracket 409 by the pulling hand module 412 after the incubation and mixing are completed, and move together in the X-axis direction, and then the cup holder pulling hand module 412 pulls the analyzing cup holders from the analyzing cup holder bracket 409 into the detection system 6.
The invention discloses a multi-wavelength time-resolved fluorescence immunoassay analyzer, which comprises an instrument rack system, a sample system, a reagent system, a cup feeding system, a filling system, a detection system, a waste storage system, an incubation system, a front shell and a rear shell of the instrument and the like, wherein the sample area, the reagent area and the analysis cup area are respectively provided with an induction function, sample transportation is completed through a sample rack bracket, transportation of an analysis cup rack is completed through a strip-shaped analysis cup rack dragging hand, transportation of a reagent is completed through a reagent rack tray, filling of the sample, the reagent 1 and the reagent 2 is completed through the filling system, after incubation and mixing are uniformly, the sample is detected, the detected waste is stored in the waste storage system, and the whole instrument has a compact structure, good reliability, large detection flux and high precision of detection. In conclusion, the multi-wavelength time-resolved fluorescence immunoassay analyzer reasonably arranges the whole structure according to methodology requirements, is efficient and stable in all aspects of motion processes, and greatly improves the flux of the analyzer by strip-shaped analysis cup detection.
Claims (10)
1. A multi-wavelength time-resolved fluorescence immunoassay analyzer is characterized by comprising an instrument rack system, a sample system, a reagent system, a cup feeding system, a filling system, a detection system, a waste storage system and an incubation system, wherein the sample system, the reagent system, the cup feeding system, the filling system, the detection system, the waste storage system and the incubation system are arranged in the instrument rack system;
the sample system is provided with a first placing area of a plurality of sample racks and is used for conveying the sample racks corresponding to the first placing area to the collecting area;
the reagent system is provided with a second placing area of the reagent rack and is used for conveying the reagent rack in the second placing area to the collecting area;
the filling system comprises a sample filling module and a reagent filling module, and the sample filling module and the reagent filling module are respectively used for filling a sample and a reagent corresponding to the collection area into an analysis cup on an analysis cup holder of the cup feeding system;
the cup feeding system is provided with a plurality of placing positions of the analyzing cup holders and is used for conveying the analyzing cup holders corresponding to the placing positions to the incubation system;
the incubation system is provided with a reciprocating module and a heating module which are respectively used for mixing and heating a reagent and a sample to complete incubation; after the incubation is finished, the cup feeding system conveys the analysis cup holder in the incubation system to the detection system;
the detection system provides a detection light source for completing the acquisition of photon signals; after the detection is complete, the cup transport system transports the analyzing cup holders within the detection system to a waste storage system.
2. The multi-wavelength time-resolved fluoroimmunoassay analyzer of claim 1, wherein the sample system comprises a sample rack spacer, a first X-axis transmission mechanism, a first Y-axis transmission mechanism, a sample rack carriage [204], a first detection unit;
the sample system is provided with a plurality of sample rack partition blocks arranged side by side and used as a plurality of first placing areas of the sample rack; the first placing area is provided with a detection unit for detecting whether the sample rack is placed at the corresponding position; the first X-axis transmission mechanism is used for driving the first Y-axis transmission mechanism to move to the position corresponding to the first placing area, and the first Y-axis transmission mechanism is used for dragging the sample rack corresponding to the first placing area to the sample rack dragging frame.
3. The multiwavelength time-resolved fluoroimmunoassay analyzer of claim 1, wherein the reagent system is provided with a second Y-axis transport mechanism and a second detection unit for detecting a transport position of the second Y-axis transport mechanism; and the second Y-axis transmission mechanism is provided with a reagent rack tray as a second placing area and is used for conveying the reagent rack tray to the collecting area.
4. The multi-wavelength time-resolved fluorescence immunoassay analyzer according to claim 1, wherein the filling system comprises a sample filling module and two reagent filling modules, and the three filling modules have the same structure and respectively comprise a second X-axis transmission mechanism, a Z-axis transmission mechanism, a third detection mechanism, a suction needle and a liquid level detector; the second X-axis transmission mechanism is used for driving the Z-axis transmission mechanism to move to the position above the corresponding acquisition area; the Z-axis transmission mechanism is used for driving the suction needle to move downwards to finish the collection of a sample or a reagent; and the third detection mechanism and the liquid level detector are respectively used for detecting the transmission position of the second X-axis transmission mechanism and the downlink position of the Z-axis transmission mechanism.
5. The multi-wavelength time-resolved fluorescence immunoassay analyzer according to claim 4, wherein the second X-axis transmission mechanism comprises a base, a third ball guide, a third ball slider, a Z-axis base plate, a fourth rotating motor, a trapezoidal screw; the Z-axis transmission mechanism comprises a fifth rotating motor, a third synchronous belt, a fourth ball guide rail and a fourth ball sliding block;
the third ball guide rail is fixed on the base, and the third ball sliding block is arranged on the third ball guide rail; the Z-axis substrate is fixed on the third ball sliding block; the fourth rotating motor is fixed on the instrument rack system and connected with a trapezoidal screw rod, and the trapezoidal screw rod is in threaded connection with the Z-axis substrate and is used for driving the Z-axis substrate to horizontally move along the X axis; the fourth ball guide rail is fixed on the Z-axis substrate, the fourth ball slide block is arranged on the fourth ball guide rail, and the suction needle and the liquid level detector are fixed on the fourth ball slide block; and the fifth rotating motor is fixed on the Z-axis substrate, and an output shaft of the fifth rotating motor is connected with the fourth ball sliding block through a third synchronous belt and used for driving the fourth ball sliding block to move up and down along the Z axis to drive the suction needle to move up and down so as to finish suction and collection and filling of samples or reagents.
6. The multi-wavelength time-resolved fluoroimmunoassay analyzer of claim 1, wherein the cup-feeding system comprises a third X-axis transmission mechanism, a third Y-axis transmission mechanism;
the third X-axis transmission mechanism comprises a bottom plate, a fifth ball guide rail, a fifth ball sliding block, a groove-shaped piece, a sixth rotating motor and a synchronous belt;
the bottom plate is provided with a plurality of baffles which are used for partitioning a plurality of placing positions of the analyzing cup holder; the fifth ball guide rail is fixed on the bottom plate, the fifth ball slide block is installed on the fifth ball guide rail, the groove-shaped piece is fixed on the fifth ball slide block, the sixth rotating motor is fixed on the instrument rack system 1, an output shaft of the sixth rotating motor is connected with the groove-shaped piece through a fourth synchronous belt and used for driving the groove-shaped piece to horizontally slide along the X axis, and the third Y-axis transmission mechanism is fixed in the groove-shaped piece and used for conveying the analysis cup holder.
7. The multi-wavelength time-resolved fluoroimmunoassay analyzer of claim 1, wherein the waste storage system comprises a fourth Y-axis transport mechanism, a waste storage cassette; and the fourth Y-axis transmission mechanism is used for driving the waste storage box to switch from the working position to the taking and placing position.
8. The multi-wavelength time-resolved fluoroimmunoassay analyzer of claim 7, wherein the fourth Y-axis transport mechanism comprises a waste storage system substrate, a seventh ball slider, a seventh ball guide, a ninth rotating motor, a fifth timing belt;
the waste storage system substrate is fixed on the instrument rack system, the seventh ball guide rail is fixed on the instrument rack system, the seventh ball slide block is installed on the seventh ball guide rail, the waste storage box is installed on the seventh ball slide block, and the ninth rotating motor rotates to drive the fifth synchronous belt to drive the waste storage box to move.
9. The multi-wavelength time-resolved fluoroimmunoassay analyzer of claim 1, wherein the incubation system comprises an incubation tray base, an incubation tray upper cover plate, a heat preservation layer, a heating module, a pressing bar, a linear reciprocating drive mechanism; the incubation disc upper cover plate is fixed on the incubation disc base, the heat preservation layer is fixed on the incubation disc upper cover plate through a pressing strip, a plurality of separation cavities are formed in the incubation disc base, the incubation disc upper cover plate, the heat preservation layer and the pressing strip form heat preservation cavities, and the heating module is arranged below the incubation disc base; the linear reciprocating driving mechanism is used for driving the incubation disc base to reciprocate along the X-axis direction.
10. The multi-wavelength time-resolved fluoroimmunoassay analyzer as claimed in claim 1, wherein the linear reciprocating drive mechanism comprises a slider-crank mechanism and an eighth rotary motor, and the rotation of the eighth rotary motor drives the slider-crank mechanism to move, thereby driving the incubation disc base to reciprocate along the X-axis direction.
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