CN110567949A - bacterial toxin detection method and treatment instrument applied to same - Google Patents
bacterial toxin detection method and treatment instrument applied to same Download PDFInfo
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- CN110567949A CN110567949A CN201910853108.8A CN201910853108A CN110567949A CN 110567949 A CN110567949 A CN 110567949A CN 201910853108 A CN201910853108 A CN 201910853108A CN 110567949 A CN110567949 A CN 110567949A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention discloses a bacterial toxin detection method and a treatment instrument applied to the method, and the technical scheme is characterized in that the treatment instrument comprises a box body, a cover plate, a heating pipe fixedly connected to the box body, an ultrasonic generator fixedly connected to the box body, a water bath heating box arranged on one side of the box body, a rotating plate rotatably connected to the top of the cover plate, a plurality of liquid injection pipes fixedly connected to one side of the top of the rotating plate, and a water absorption sponge arranged on one side of the top of the rotating plate, which is far away from the liquid injection pipes; the detection method comprises the following steps: a. preparing a working solution; b. heating and disinfecting the reaction plate; c. adding the solution; d. vibrating and mixing; e. heating in water bath; f. cleaning; g. dripping color development liquid and stop solution; h. detecting by an instrument; the diluent, the standard solution, the antibody enzyme conjugate, the developing solution A, the developing solution B and the stop solution are all arranged in the box body when being added, and the box body is heated and sterilized before air stripping, so that the influence of bacteria in the air on the accuracy of a test result is reduced.
Description
Technical Field
The invention relates to the technical field of food detection, in particular to a bacterial toxin detection method and a processor applied to the method.
Background
with the continuous development of the current society, the food safety becomes a problem which is widely concerned in the current society, and the food sampling detection is an important mode for detecting the food safety.
In food detection, when residues of escherichia coli or salmonella in food are detected, mostly, a proper amount of methanol solution is added into a crushed sample, then the sample solution is diluted and then is placed into reaction holes of a reaction plate, then a standard solution is added into the standard holes, then antibody enzyme conjugates are added into all the holes, after uniform mixing, water bath at 37 ℃ is carried out for 30 minutes, then the liquid is cleaned by washing liquid, finally, excessive water is sucked through absorbent paper to be dry, so that antigen antibody complexes generated by reaction are separated from the liquid and attached to the holes, then developing solution A and developing solution B are sequentially added into each hole, after uniform mixing, water bath at 37 ℃ is carried out for ten minutes, then stop solution is added into each hole, and after uniform mixing, the stop solution is placed into a drug residue detector for detection.
However, in the experiment, when the diluent, the standard solution, the antibody enzyme conjugate, the developing solution A, the developing solution B and the stopping solution are added, the operation is manually carried out in the air, so that various bacteria in the air are easy to float into the holes, and the accuracy of the test result is influenced.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a treatment instrument, wherein a diluent, a standard solution, an antibody enzyme conjugate, a developing solution A, a developing solution B and a stopping solution are all arranged in a box body when being added, and the box body is heated and sterilized before air is lifted, so that the influence of bacteria in the air on the accuracy of a test result is reduced.
In order to achieve the purpose, the invention provides the following technical scheme: a treatment instrument comprises a box body with an opening at the top, a cover plate covering the top of the box body, a heating pipe fixedly connected to the inner wall of the box body, an ultrasonic generator fixedly connected to the inner wall of the box body, a support frame arranged in the box body and used for supporting a reaction plate, a water bath heating box arranged on one side of the box body and used for conveying constant-temperature water to the box body, a rotating plate rotatably connected to the top of the cover plate and penetrating through the cover plate in a circular manner, a plurality of liquid injection pipes fixedly connected to one side of the top of the rotating plate and penetrating through the rotating plate, a pipe plug inserted into one end of the top of the liquid injection pipe, and a water absorption sponge arranged on one side of the top of the rotating plate, which is far away;
Rotate the rotor plate, can rotate in proper order to annotate liquid pipe and sponge that absorbs water directly over the reaction dish, annotate the liquid pipe and be located directly over the reaction plate when, all correspond one and annotate the liquid pipe directly over each reaction hole, absorb water the sponge and be located directly over the reaction dish when, the sponge that absorbs water can pass rotor plate and reaction plate in contact with each other downwards.
By adopting the technical scheme, the reaction plate is placed on the support frame, then the cover plate is covered, the air in the box body is heated through the heating pipe to kill the air in the box body and bacteria on the reaction plate, then the liquid injection pipe is rotated to the top of the reaction plate after the air in the box body and the reaction plate are cooled to room temperature, so that the dropper penetrates through the liquid injection pipe to drip liquid to be added onto the reaction plate, and the liquid is vibrated and mixed through ultrasonic waves emitted by the ultrasonic generator during vibration; when the reaction plate is cleaned, the water pipe is connected to the top of the liquid injection pipe to clean the reaction plate, and when the reaction plate is wiped dry, the water absorption sponge is rotated to the top of the reaction plate and penetrates through the rotating plate to be in contact with the top of the reaction plate, so that the water on the reaction plate is absorbed; when heating in a water bath, circulating constant-temperature water between the box body and the water bath heating box through the water bath heating box to realize constant-temperature water bath heating of the reaction plate in the box body; therefore, when the diluent, the standard solution, the antibody enzyme conjugate, the developing solution A, the developing solution B and the stop solution are added, the mixture is positioned in the box body, and the box body is heated and sterilized before air stripping, so that the influence of bacteria in the air on the accuracy of test results is reduced.
the invention is further configured to: one side of the top of the rotating plate, which is far away from the liquid injection pipe, is provided with a abdicating hole which penetrates through the rotating plate along the vertical direction, and the rotating plate is rotated, so that the abdicating hole can be positioned right above the reaction plate;
The top of the rotating plate is provided with a sliding plate which can slide on the rotating plate along the horizontal direction, the sliding plate is provided with a through hole which has the same size and shape as the abdicating hole, and the sliding plate can enable the abdicating hole and the through hole to be superposed with each other;
The water absorption sponge is arranged above the abdicating hole.
Through adopting above-mentioned technical scheme, when not needing to absorb water the clearance to the reaction plate, slide the sliding plate, shelter from the hole of stepping down through the sliding plate for outside air can't enter into the middle of the box through the hole of stepping down, when the sponge that absorbs water clears up the reaction plate, slide the sliding plate, make the through-hole with step down the hole and coincide it each other.
The invention is further configured to: the water absorption sponge fixing device is characterized in that two guide rods which are vertically arranged are fixedly connected to the top of the rotating plate, the two guide rods are respectively located at two ends of the through hole, a flat plate is arranged at the top of the through hole, the two guide rods respectively penetrate through two ends of the flat plate, a fixing rod which is vertically arranged is fixedly connected to the bottom of the flat plate, and the water absorption sponge is fixedly connected to the bottom of the fixing rod.
Through adopting above-mentioned technical scheme, when using the sponge that absorbs water to clear up the water on the reaction plate, push down the flat board for the sponge that absorbs water slides down, and with dull and stereotyped top in contact with each other, absorb the water at reaction plate top dry through the sponge that absorbs water.
the invention is further configured to: the fixed rod is sleeved with a spring positioned between the flat plate and the sliding plate, and the spring is in a compressed state.
through adopting above-mentioned technical scheme, after absorbing the water on the flat board through the sponge that absorbs water futilely, the contact is spacing to the flat board, under the elastic force effect of spring, flat board and the sponge that absorbs water resume the normal position.
The invention is further configured to: the top of the rotating plate is fixedly connected with two guide blocks which are respectively positioned at two ends of the yielding hole, the bottom of one side, close to each other, of each guide block is provided with a guide groove, and the sliding plate is close to two sides of each guide block and is respectively inserted into the two guide grooves.
through adopting above-mentioned technical scheme, can lead the slip direction of sliding plate through setting up guide block and guide way.
the invention is further configured to: the bottom of the water-absorbing sponge is fixedly connected with a plurality of bumps, and when the water-absorbing sponge is positioned right above the reaction plate, one bump is corresponding to each reaction hole.
Through adopting above-mentioned technical scheme, when the sponge that absorbs water cleared up the reaction plate, the lug can stretch into in the middle of the reaction hole at reaction plate top to make the moisture in the reaction hole can be more quick by the clean up.
The invention is further configured to: the bottom of one side of the box body is fixedly connected with a circulating pump, the circulating pump is fixedly connected with a circulating pipe, and one end of the circulating pipe, which is far away from the circulating pump, is fixedly connected to the water bath heating box;
the position fixedly connected with working shaft that water bath heating cabinet one side is close to the bottom, fixedly connected with delivery pipe on the working shaft, the one end fixed connection of delivery pipe is close to the position at top in box one side.
through adopting above-mentioned technical scheme, the water after the working shaft will heat is sent into in the middle of the box, and the circulating pump of bottom half sends water into the water bath heating case in the middle of, through the circulation of water, realizes the constant temperature heating.
The invention is further configured to: a plurality of heating rods are fixedly connected in the water bath heating box, one end of the circulating pipe, which is connected with the water bath heating box, is positioned above the heating rods, and the water supply pump is positioned below the heating rods.
through adopting above-mentioned technical scheme, the water level that the circulating pipe sent into in the water bath heating case is above the heating rod, passes the heating rod after the heating rod heating and is taken out by the working shaft, has realized the circulation heating to water to make the temperature of water can maintain invariable.
Another object of the present invention is to provide a method for detecting bacterial toxins, which reduces the influence of bacteria in the air on the accuracy of the test result when detecting bacteria, wherein the method comprises the following steps:
a. Preparing a working solution: crushing 10g of sample, adding 20ml of 70% methanol solution, shaking and mixing, and filtering to obtain a sample solution;
b. Heating and disinfecting a reaction plate: placing the reaction plate on a support frame, and heating the box body in a heating pipe, wherein the heating temperature is controlled to be 100-110 ℃, and the heating time is 10 min; after heating, waiting for the box body and the reaction plate to be cooled to room temperature;
c. Adding a solution: rotating the rotating plate to enable the upper part of the reaction hole corresponding to the liquid injection pipe to be positioned, then pulling out the pipe plug, penetrating the dropper through the liquid injection pipe, adding 25 microliters of sample liquid or 50 microliters of standard liquid into the corresponding hole, then dropwise adding 25 microliters of sample diluent into the reaction hole added with the sample solution through the dropper, and then dropwise adding 50 microliters of antibody-enzyme conjugate into each hole;
d. Vibrating and mixing: covering a pipe plug, transmitting ultrasonic waves through an ultrasonic transmitter, and mixing the solution in the hole;
e. Heating in water bath: the water bath heating box heats water to 37 ℃, the water is conveyed into the box body through the water supply pipe, so that the bottom of the reaction plate is contacted with the water, the circulation of the water in the box body and the water bath heating box is realized through the circulating pipe and the water supply pipe, and the reaction plate is heated in a water bath for 30 min;
f. cleaning: after heating, the water supply pipe stops supplying water, the circulating pipe conveys all water in the box body into the water bath heating box, then the pipe plug is pulled out, the water pipe is connected to the liquid injection pipe, the water pipe is used for washing the hole in the reaction plate, after the hole is washed completely, the water pipe is detached, the pipe plug is plugged, the rotating plate is rotated, the abdicating hole is rotated to be right above the reaction plate, the sliding plate is slid, the through hole is overlapped with the abdicating hole, the flat plate is pressed downwards, the water absorption sponge is contacted with the top of the reaction plate, water in the reaction plate and the reaction hole is absorbed, then the water absorption sponge and the sliding plate are restored to the original position, and water generated by cleaning is pumped into the water;
g. dripping a color development liquid and a stop solution: rotating the rotating plate to enable the liquid injection pipe to be above the corresponding reaction hole, then pulling off the pipe plug, and sequentially dripping a developing solution A50 microliter and a developing solution B50 microliter into the reaction hole through the liquid injection pipe; then, uniformly mixing the developing solution by using ultrasonic waves, repeating the water bath heating step, and heating the reaction plate in a water bath for 10min at the heating temperature of 37 ℃;
h. detecting by an instrument: and (4) placing the reaction plate after the reaction into a medicine residue detector for detection.
By adopting the technical scheme, the diluent, the standard solution, the antibody enzyme conjugate, the developing solution A, the developing solution B and the stop solution are all in the box body when being added, and the air in the box body is heated and sterilized before being stripped, so that the influence of bacteria in the air on the accuracy of a test result is reduced.
in summary, compared with the prior art, the invention has the following beneficial effects:
1. According to the invention, the box body, the rotating plate, the liquid injection pipe, the water absorption sponge and the water bath heating box are arranged, the diluent, the standard liquid, the antibody enzyme conjugate, the color developing liquid A, the color developing liquid B and the stop solution are all arranged in the box body when being added, and the box body is heated and sterilized before air is extracted from the box body, so that the influence of bacteria in the air on the accuracy of a test result is reduced;
2. according to the reaction plate cleaning device, the bump is arranged, and when the reaction plate is cleaned by the water absorption sponge, the bump can extend into the reaction hole in the top of the reaction plate, so that water in the reaction hole can be cleaned more quickly.
Drawings
FIG. 1 is an isometric view of the completed structure of the first embodiment;
FIG. 2 is a sectional view of the case according to the first embodiment;
FIG. 3 shows a cover plate and a supporting frame according to the first embodiment;
FIG. 4 is an enlarged view of section A of FIG. 1;
fig. 5 is a sectional view of the water bath heating cabinet according to the first embodiment.
In the figure: 1. a box body; 11. a circulation pump; 12. a circulation pipe; 13. heating a tube; 14. an ultrasonic generator; 2. a cover plate; 3. a rotating plate; 31. a hole of abdication; 32. a guide bar; 321. a spring; 33. a flat plate; 34. fixing the rod; 4. a liquid injection pipe; 41. a pipe plug; 5. a water-absorbing sponge; 51. a bump; 6. a water bath heating box; 61. a water supply pump; 62. a water supply pipe; 63. a heating rod; 7. a support frame; 71. a connecting rod; 8. a sliding plate; 81. a through hole; 9. a guide block; 91. a guide groove.
Detailed Description
the first embodiment is as follows: a treatment instrument, see attached figures 1 and 2, comprises a box body 1 with an open top, a cover plate 2 covering the top of the box body 1, a heating pipe 13 fixedly connected with the inner wall of the box body 1, an ultrasonic generator 14 fixedly connected with the inner wall of the box body 1, a support frame 7 arranged in the box body 1 and used for supporting a reaction plate, a water bath heating box 6 arranged at one side of the box body 1 and used for conveying constant-temperature water into the box body 1, a rotating plate 3 rotatably connected with the top of the cover plate 2 and penetrating through the cover plate 2, a plurality of liquid injection pipes 4 fixedly connected with one side of the top of the rotating plate 3 and penetrating through the rotating plate 3, a pipe plug 41 inserted at one end of the top of the liquid injection pipe 4, a water absorption sponge 5 arranged at one side of the top of the rotating plate 3, which is far away from the liquid injection pipe 4 and used for absorbing residual water on the reaction plate, a circulating pump 11 fixedly connected, A water supply pump 61 fixedly connected to the bottom of the water bath heating tank 6 on one side and a water supply pipe 62 fixedly connected to the water supply pump 61; the rotating plate 3 is arranged in a circular shape, the axis of the rotating plate 3 is vertically arranged, and the rotating plate 3 rotates on the cover plate 2 along the axis of the rotating plate 3; one end of the circulating pipe 12 departing from the circulating pump 11 is fixedly connected to one side of the water bath heating tank 6 close to the top, and one end of the circulating pipe 12 departing from the circulating pump 11 is fixedly connected to one side of the tank body 1 close to the top. Rotate rotor plate 3, can rotate in proper order to annotate liquid pipe 4 and absorb water sponge 5 directly over the reaction dish, annotate liquid pipe 4 and be located directly over the reaction dish when, all correspond one directly over each reaction hole and annotate liquid pipe 4, absorb water sponge 5 and be located directly over the reaction dish when, absorb water sponge 5 can pass rotor plate 3 and reaction plate in contact with each other downwards.
When a solution is dripped onto the reaction plate, the rotating plate 3 is rotated, so that the liquid injection pipes 4 are positioned above the reaction plate, the liquid injection pipes 4 correspond to the reaction holes one by one, then the pipe plugs 41 are pulled out, and the burette is inserted into the liquid injection pipe 4 to drip the solution into the reaction holes; when the reaction plate needs to be cleaned, the liquid injection pipes 4 correspond to the reaction holes one by one, then the liquid injection pipes 4 are connected with the water pipes, and water is sent to the reaction plate through the liquid injection pipes 4 to clean the reaction plate; when the reaction plate is wiped dry, the water-absorbing sponge 5 is rotated to the top of the reaction plate, and the water-absorbing sponge 5 penetrates through the rotating plate 3 to be in contact with the top of the reaction plate, so that the water on the reaction plate is absorbed; when heating in a water bath, constant temperature water circulates between the box body 1 and the water bath heating box 6 through the water bath heating box 6, and constant temperature water bath heating of the reaction plate in the box body 1 is realized.
referring to fig. 3, four vertically arranged connecting rods 71 are fixedly connected to positions, close to four corners, of the bottom of the cover plate 2, and one ends of the bottoms of the four connecting rods 71 are respectively and fixedly connected to four corners of the top of the supporting net.
referring to the attached drawings 1 and 4, a yielding hole 31 penetrating through the rotating plate 3 is formed in the vertical direction on one side of the top of the rotating plate 3, which is far away from the liquid injection pipe 4, and the rotating plate 3 is rotated, so that the yielding hole 31 can be positioned right above the reaction plate; the top of the rotating plate 3 is provided with a sliding plate 8 which can slide on the rotating plate 3 along the horizontal direction, the top of the rotating plate 3 is fixedly connected with two guide blocks 9 which are respectively positioned at two ends of the abdicating hole 31, the bottom of one side, close to each other, of each guide block 9 is provided with a guide groove 91, and the two sides, close to the guide blocks 9, of the sliding plate 8 are respectively inserted into the two guide grooves 91; the sliding plate 8 is provided with a through hole 81 with the same size and shape as the abdicating hole 31, and the sliding plate 8 can enable the abdicating hole 31 and the through hole 81 to be overlapped; the top of the rotating plate 3 is fixedly connected with two vertically arranged guide rods 32, the two guide rods 32 are respectively positioned at two ends of the through hole 81, the top of the through hole 81 is provided with a flat plate 33, the two guide rods 32 respectively penetrate through two ends of the flat plate 33, the bottom of the flat plate 33 is fixedly connected with a vertically arranged fixing rod 34, and the water absorption sponge 5 is fixedly connected at the bottom of the fixing rod 34. The fixed rod 34 is sleeved with a spring 321 positioned between the flat plate 33 and the sliding plate 8, and the spring 321 is in a compressed state; the bottom of the water absorption sponge 5 is fixedly connected with a plurality of bumps 51, the bumps 51 are made of the same material as the water absorption sponge 5, and when the water absorption sponge 5 is positioned right above the flat plate 33, one bump 51 corresponds to each reaction hole.
When the flat plate 33 needs to be cleaned, the rotating plate 3 is rotated, the abdicating hole 31 is located right above the flat plate 33, then the sliding plate 8 is slid, the abdicating hole 31 and the through hole 81 are overlapped, then the flat plate 33 is pressed downwards, the water absorbing sponge 5 penetrates through the abdicating hole 31 and the through hole 81 to be in contact with the flat plate 33, and after water absorption is finished, the flat plate 33 and the water absorbing sponge 5 return to the original position under the elastic force action of the spring 321. When the cleaning is not needed, the sliding plate 8 slides, and the copper drum sliding plate 8 shields the abdicating hole 31.
referring to fig. 5, a plurality of heating rods 63 are fixedly connected in the water bath heating tank 6, one end of the circulating pipe 12 connected with the water bath heating tank 6 is positioned above the heating rods 63, and the water supply pump 61 is positioned below the heating rods 63; the water fed into the water bath heating tank 6 by the circulating pipe 12 enters the water bath heating tank 6, passes through the heating pipe 13 for heating, and is pumped out by the water supply pump 61, so that the water is circularly heated, and the water can be kept at a constant temperature.
The working principle of the processor when in use is as follows: when a solution is dripped onto the reaction plate, the rotating plate 3 is rotated, so that the liquid injection pipes 4 are positioned above the reaction plate, the liquid injection pipes 4 correspond to the reaction holes one by one, then the pipe plugs 41 are pulled out, and the burette is inserted into the liquid injection pipe 4 to drip the solution into the reaction holes; when the reaction plate needs to be cleaned, the liquid injection pipes 4 correspond to the reaction holes one by one, then the liquid injection pipes 4 are connected with the water pipes, and water is sent to the reaction plate through the liquid injection pipes 4 to clean the reaction plate; when the flat plate 33 needs to be cleaned, the rotating plate 3 is rotated to enable the abdicating hole 31 to be positioned right above the flat plate 33, then the sliding plate 8 is slid to enable the abdicating hole 31 and the through hole 81 to be coincided with each other, then the flat plate 33 is pressed downwards to enable the water absorbing sponge 5 to penetrate through the abdicating hole 31 and the through hole 81 to be contacted with the flat plate 33, and after water absorption is finished, the flat plate 33 and the water absorbing sponge 5 return to the original positions under the elastic force of the spring 321; when heating in a water bath, constant temperature water circulates between the box body 1 and the water bath heating box 6 through the water bath heating box 6, and constant temperature water bath heating of the reaction plate in the box body 1 is realized.
Example two: a method of detecting bacterial toxins comprising the steps of:
a. preparing a working solution: crushing 10g of sample, adding 20ml of 70% methanol solution, shaking and mixing, and filtering to obtain a sample solution;
b. heating and disinfecting a reaction plate: placing the reaction plate on a support frame 7, and then heating the interior of the box body 1 through a heating pipe 13, wherein the heating temperature is controlled to be 100-110 ℃, and the heating time is 10 min; after heating, waiting for the box body 1 and the reaction plate to be cooled to room temperature;
c. Adding a solution: rotating the rotating plate 3 to enable the upper part of the reaction hole corresponding to the liquid injection pipe 4 to be pulled out, then pulling the pipe plug 41, enabling the dropper to penetrate through the liquid injection pipe 4, adding 25 microliters of sample liquid or 50 microliters of standard liquid into the corresponding hole, then dropwise adding 25 microliters of sample diluent into the reaction hole added with the sample liquid through the dropper, and then dropwise adding 50 microliters of antibody-enzyme conjugate into each hole;
d. Vibrating and mixing: covering the pipe plug 41, transmitting ultrasonic waves through an ultrasonic transmitter, and mixing the solution in the hole;
e. heating in water bath: the water bath heating box 6 heats water to 37 ℃, the water is conveyed into the box body 1 through the water supply pipe 62, so that the bottom of the reaction plate is contacted with the water, the circulation of the water in the box body 1 and the water bath heating box 6 is realized through the circulating pipe 12 and the water supply pipe 62, and the reaction plate is heated in a water bath for 30 min;
f. Cleaning: after heating, the water supply pipe 62 stops supplying water, the circulating pipe 12 conveys all water in the box body 1 to the water bath heating box 6, then the pipe plug 41 is pulled out, the water pipe is connected to the liquid injection pipe 4, the hole in the reaction plate is washed by the water pipe, after the water pipe is washed completely, the water pipe is detached, the pipe plug 41 is plugged, the rotating plate 3 is rotated, the abdicating hole 31 is rotated to be right above the reaction plate, the sliding plate 8 is slid, the through hole 81 is overlapped with the abdicating hole 31, the flat plate 33 is pressed downwards, the water absorption sponge 5 is contacted with the top of the reaction plate, water in the reaction plate and the reaction hole is absorbed, then the water absorption sponge 5 and the sliding plate 8 are restored to the original positions, and water generated by washing is pumped into the water bath heating box 6 by the;
g. Dripping a color development liquid and a stop solution: rotating the rotating plate 3 to enable the liquid injection pipe 4 to be above the corresponding reaction hole, then pulling the pipe plug 41 off, and sequentially dripping 50 microliters of color development liquid A and 50 microliters of color development liquid B into the reaction hole through the liquid injection pipe 4; then, uniformly mixing the developing solution by using ultrasonic waves, repeating the water bath heating step, and heating the reaction plate in a water bath for 10min at the heating temperature of 37 ℃;
h. detecting by an instrument: and (4) placing the reaction plate after the reaction into a medicine residue detector for detection.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (9)
1. a processor, characterized by: comprises a box body (1) with an opening at the top, a cover plate (2) covered on the top of the box body (1), a heating pipe (13) fixedly connected on the inner wall of the box body (1), an ultrasonic generator (14) fixedly connected on the inner wall of the box body (1), and a support frame (7) arranged in the box body (1) and used for supporting a reaction plate, the reaction plate comprises a water bath heating box (6) which is arranged on one side of a box body (1) and used for conveying constant-temperature water into the middle of the box body (1), a rotating plate (3) which is rotatably connected to the top of a cover plate (2) and penetrates through the cover plate (2) and is circularly arranged, a plurality of liquid injection pipes (4) which are fixedly connected to one side of the top of the rotating plate (3) and penetrate through the rotating plate (3), a pipe plug (41) which is inserted into one end of the top of each liquid injection pipe (4), and a water absorption sponge (5) which is arranged on one side, which is far away from the liquid injection pipes (4), of the top of each rotating plate (;
Rotate rotor plate (3), can rotate in proper order and annotate liquid pipe (4) and absorb water sponge (5) directly over to the reaction dish, annotate liquid pipe (4) and be located directly over the reaction dish when, all correspond one and annotate liquid pipe (4) directly over each reaction hole, absorb water sponge (5) and be located directly over the reaction dish when, absorb water sponge (5) can pass rotor plate (3) and reaction plate in contact with each other downwards.
2. The processor of claim 1, wherein: one side of the top of the rotating plate (3), which is far away from the liquid injection pipe (4), is provided with a yielding hole (31) which penetrates through the rotating plate (3) along the vertical direction, and the rotating plate (3) is rotated, so that the yielding hole (31) can be positioned right above the reaction plate;
The top of the rotating plate (3) is provided with a sliding plate (8) capable of sliding on the rotating plate (3) along the horizontal direction, the sliding plate (8) is provided with a through hole (81) with the same size and shape as the abdicating hole (31), and the sliding plate (8) can enable the abdicating hole (31) and the through hole (81) to be overlapped with each other;
The water absorption sponge (5) is arranged above the abdicating hole (31).
3. The processor of claim 2, wherein: the water absorption sponge is characterized in that the top of the rotating plate (3) is fixedly connected with two vertically arranged guide rods (32), the two guide rods (32) are respectively located at two ends of a through hole (81), a flat plate (33) is arranged at the top of the through hole (81), the two guide rods (32) respectively penetrate through two ends of the flat plate (33), a fixing rod (34) which is vertically arranged is fixedly connected to the bottom of the flat plate (33), and the water absorption sponge (5) is fixedly connected to the bottom of the fixing rod (34).
4. the processor of claim 3, wherein: the fixed rod (34) is sleeved with a spring (321) positioned between the flat plate (33) and the sliding plate (8), and the spring (321) is in a compressed state.
5. The processor of claim 2, wherein: two guide blocks (9) which are respectively positioned at two ends of the abdicating hole (31) are fixedly connected to the top of the rotating plate (3), a guide groove (91) is formed in the bottom of one side, close to each other, of each guide block (9), and the sliding plate (8) is inserted into the two guide grooves (91) respectively close to two sides of each guide block (9).
6. the processor of claim 1, wherein: the bottom of the water absorption sponge (5) is fixedly connected with a plurality of bumps (51), and when the water absorption sponge (5) is positioned right above the reaction plate, one bump (51) corresponds to the position right above each reaction hole.
7. The processor of claim 1, wherein: the bottom of one side of the box body (1) is fixedly connected with a circulating pump (11), the circulating pump (11) is fixedly connected with a circulating pipe (12), and one end of the circulating pipe (12) departing from the circulating pump (11) is fixedly connected to the water bath heating box (6);
The position fixedly connected with feed pump (61) that water bath heating cabinet (6) one side is close to the bottom, fixedly connected with delivery pipe (62) on feed pump (61), the one end fixed connection of delivery pipe (62) is in the position that box (1) one side is close to the top.
8. the processor of claim 7, wherein: a plurality of heating rods (63) are fixedly connected in the water bath heating box (6), one end of the circulating pipe (12) connected with the water bath heating box (6) is located above the heating rods (63), and the water supply pump (61) is located below the heating rods (63).
9. a method for detecting bacterial toxins using the processor of any one of claims 1-8, wherein: the method comprises the following steps:
a. Preparing a working solution: crushing 10g of sample, adding 20ml of 70% methanol solution, shaking and mixing, and filtering to obtain a sample solution;
b. Heating and disinfecting a reaction plate: placing the reaction plate on a support frame (7), and then heating the interior of the box body (1) by a heating pipe (13), wherein the heating temperature is controlled at 100-110 ℃, and the heating time is 10 min; after heating, waiting for the box body (1) and the reaction plate to be cooled to room temperature;
c. adding a solution: rotating the rotating plate (3) to enable the liquid injection pipe (4) to be positioned above the corresponding reaction hole, then pulling the pipe plug (41) down, enabling the dropper to penetrate through the liquid injection pipe (4), adding 25 microliters of sample liquid or 50 microliters of standard liquid into the corresponding hole, then dropwise adding 25 microliters of sample diluent into the reaction hole added with the sample solution through the dropper, and then dropwise adding 50 microliters of antibody-enzyme conjugate into each hole;
d. Vibrating and mixing: covering a pipe plug (41), and transmitting ultrasonic waves through an ultrasonic transmitter to mix the solution in the hole;
e. heating in water bath: the water bath heating box (6) heats water to 37 ℃, water is conveyed into the box body (1) through the water supply pipe (62) to enable the bottom of the reaction plate to be contacted with the water, the circulation of the water in the box body (1) and the water bath heating box (6) is realized through the circulating pipe (12) and the water supply pipe (62), and the reaction plate is heated in a water bath for 30 min;
f. cleaning: after heating, the water supply pipe (62) stops supplying water, the circulating pipe (12) conveys all the water in the box body (1) to the water bath heating box (6), then the pipe plug (41) is pulled out, the water pipe is connected to the liquid injection pipe (4), the water pipe is used for washing the hole in the reaction plate, after the water is washed completely, the water pipe is detached, the pipe plug (41) is plugged, the rotating plate (3) is rotated, the abdicating hole (31) is rotated to be right above the reaction plate, the sliding plate (8) is slid, so that the through hole (81) is overlapped with the abdicating hole (31), the flat plate (33) is pressed downwards, the water absorption sponge (5) is contacted with the top of the reaction plate, the water in the reaction plate and the reaction hole is absorbed, then the water absorption sponge (5) and the sliding plate (8) return to the original position, and the water generated by cleaning is pumped into the water bath heating box (6) by the;
g. Dripping a color development liquid and a stop solution: rotating the rotating plate (3) to enable the liquid injection pipe (4) to be positioned above the corresponding reaction hole, then pulling off the pipe plug (41), and sequentially dripping 50 microliters of color development liquid A and 50 microliters of color development liquid B into the reaction hole through the liquid injection pipe (4); then, uniformly mixing the developing solution by using ultrasonic waves, repeating the water bath heating step, and heating the reaction plate in a water bath for 10min at the heating temperature of 37 ℃;
h. detecting by an instrument: and (4) placing the reaction plate after the reaction into a medicine residue detector for detection.
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