CN105876160A - Method for preparing molasses sugar acidification and glycolysis feed - Google Patents

Method for preparing molasses sugar acidification and glycolysis feed Download PDF

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Publication number
CN105876160A
CN105876160A CN201610285259.4A CN201610285259A CN105876160A CN 105876160 A CN105876160 A CN 105876160A CN 201610285259 A CN201610285259 A CN 201610285259A CN 105876160 A CN105876160 A CN 105876160A
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acid
fermentation
liquid
wet basis
molasses
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程勐万里
刘萱仪
孟龙
薛芹
程茂基
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ANHUI WULIANGTAI BIOLOGICAL ENGINEERING Co Ltd
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ANHUI WULIANGTAI BIOLOGICAL ENGINEERING Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention relates to a method for preparing a molasses sugar acidification and glycolysis feed. The method comprises the following steps: introducing the molasses from which iron is removed by ion exchange, to a citric acid-producing strain and fermenting to obtain a citric acid residue wet base and a citric acid fermenting solution; introducing molasses from which iron is removed by ion exchange, to a lactic acid bacteria strain, fermenting and acidifying to prepare a lactic acid residue wet base and a lactic acid fermenting solution, and then concentrating one or more of the lactic acid fermenting solution and the citric acid fermenting solution to prepare an organic acid concentrated solution; mixing one or more of the citric acid residue wet base and the lactic acid residue wet base with feed raw materials, a strain solution and enzymes, and then carrying out enzymatic hydrolysis fermentation to prepare glycolysis dregs; mixing the organic acid concentrate and the glycolysis dregs with one or more of feed inorganic acid, inorganic acid salts, sugars, oil, Chinese herbs and vitamins to prepare the molasses sugar acidification and glycolysis feed. The feed prepared by the method disclosed by the invention contains the nutrients, such as organic acids, small peptides, oligosaccharide and probiotics and has the characteristics of resistance to diarrhea, good palatability, easiness of digestion and the like.

Description

The acidifying of a kind of molasses saccharide and the preparation method of zymolysis feedstuff
Technical field
The invention belongs to feedstuff, enzyme preparation and microbial technology field, be specifically related to a kind of molasses saccharide acidifying Preparation method with zymolysis feedstuff.
Background technology
In recent years, food safety and environmental pollution extremely governments at all levels of various countries extensive concern, it is good for concerning the common people Health and life security.Livestock product safety requires feedstuff no drug residue, environmental protection requirement feedstuff low stain.Past We talk feedstuff is " effectively, safety ", present National agricultural portion emphasizes again and again ' safely, effectively, low stain ". It is known that antibiotic is since coming out, it takes precautions against disease for poultry and stimulating growth have received good really Good effect.But As time goes on, the defect of antibiotic is completely exposed: one is that antibiotic easily remains In the animal products such as meat, egg and milk, cause human body Liver and kidney function burden or canceration occurs, harm Human health;Two is to make livestock products local flavor decline;Three is to cause mankind itself's Drug resistance to increase, and pollutes The natural environment such as soil and water source.Therefore, some countries have prohibited use.1974, the European Community prohibited Only using penicillin, tetracycline in feedstuff, in December, 1998 forbids using spiramycin, bar in feedstuff Bacterium peptide zinc, virginiamycin and the happy mycin of peptide, forbid using chlorine fluorine benzyl gland fast in feedstuff in October, 1999 Purine (arprinocide) and isopropyl nitre reach azoles (dinitolmide).Therefore, citric acid, lactic acid, Rhizoma Corydalis are used Rope acid, probiotic bacteria, oligosaccharide and active small peptide substitute or reduce use antibiotic, produce safe animal products National governments are enjoyed to pay close attention to.
China is populous nation, Ye Shi aquaculture big country.2015, the animal husbandry output value reached 3.2 trillion yuan, Meat, fowl egg, milk and output of aquatic products respectively reach 84,540,000 tons, 28,000,000 tons, 38,000,000 tons and Fifty-five million ton.Along with the development of aquaculture, feed industrial development speed is surprising.According to Ministry of Agriculture's statistical number According to, within 2015, national commercial feed yield reaches 2.18 hundred million tons.Wherein, complete feed 1.79 hundred million tons, concentrate 21,510,000 tons of feedstuff, premix material 6,400,000 tons, including pig feed yield 89,740,000 tons, fowl material yield 90,000,000 Ton, aquatic feeds 53,000,000 tons, 8,500,000 tons of cattle and sheep feedstuff, 3,500,000 tons of the feedstuff such as rabbit Nyctereutes procyonoides house pet.
It is known that the difficult point of the exploitation of feed product, development is the feed for piglet.How to improve children's age Molasses feed palatability, digestibility and reduction or minimizing antinutritional factor become Animal nutrition scholar in recent years and amass The problem that pole is inquired into.For suckling piglet, its metabolism is vigorous, and relative growth is rapid, to diet nutrient The requirement of material is high, but its gastral function is unsound, gastral weight and volume is less and sends out the region between the heart and the diaphragm not Completely, gastric acid secretion is not enough, and gastrointestinal micro-flora is variable, and it is unsound that Digest enzyme sends out the region between the heart and the diaphragm, digestive enzyme Hyposecretion.Suckling piglet protein digestibility is bad, and protein easily produces some spoilage product at hindgut, cadaverine, Putrescine, histamine etc., stimulate intestinal mucosa, damages intestinal villi film, causes intestinal contents osmotic pressure to raise, intestinal Road dehydration causes diarrhoea.To the piglet before vegetable protein allergy, 8 week old, allergy easy to vegetable protein causes Intestinal mucosa is impaired, the impact absorption to nutrient, and antibacterial is easily invaded and causes diarrhoea.Therefore, by fermentation, The biotechnology such as enzymolysis, nutrition is high, easy to digest in exploitation, good palatability, rich in citric acid, lactic acid, Rhizoma Corydalis Rope acid and probiotic bacteria, oligosaccharide, the feed for piglet of active small peptide be one extremely urgent and there is important meaning The major issue of justice.
Molasses are the side-products of sugar industry.Molasses contain a small amount of thick protein, generally 3%~6%, belong to more In non-protein nitrogen class, such as ammonia, amide and nitrate etc., and amino-acid nitrogen only accounts for 38%~50%, and non-must Needing aminoacid such as aspartic acid, content of glutamic acid more, therefore protein biological value is relatively low, but sky Aspartic Acid and glutamic acid are delicious amino acid, therefore can stimulating animal appetite significantly in animal feed.
Molasses be mainly composed of saccharide, cane molasses contain sucrose about 24%~36%, other sugar about 12%~24%; Saccharide contained by beet molasses is almost all sucrose, and about 47% more than.Additionally contain the solubility glue of 3%~4% Body, predominantly xylane, araban and pectin etc..
The content of mineral substances of molasses is higher, about 8%~10%, but calcium, phosphorus content are the highest, and cane molasses is the highest In beet molasses.Potassium, chlorine, sodium, content of magnesium height in mineral element, therefore molasses have laxative qualits.Typically Molasses vitamin content is low, but in cane molasses, pantothenic acid content is higher, reaches 37mg/kg, biotin content in addition The most considerable.
At present, molasses spray to have on bean cake matured product, referred to as molasses bean cake, also have and sending out The fermented feed product adding a small amount of molasses to ferment together in ferment enzymolysis feed and to develop or research or report Road.But, have no the product of molasses fermented production composite organic acid acidulant or research or report.
It is mainly Caulis Sacchari sinensis and Radix Betae containing sugar products.Caulis Sacchari sinensis originates in the torrid zone, subtropical zone, and in its stem, sugar content is 10~20%.Radix Betae is cultivated at cold district, and in its root, sugar content is also 10~20%.Both sugar contents Quite.But the most of sugaring are Caulis Sacchari sinensis.The yield of Saccharum Sinensis Roxb. about 65% is from Caulis Sacchari sinensis in the world, the most about 35% takes from Radix Betae, so Saccharum Sinensis Roxb. is also referred to as sucrose.
Feeder acidulant reduces feedstuff pH value in digestive tract as a kind of, provides the suitableeest digestion for animal The new additive agent of road environment, is the most at home and abroad used widely.Acidulant be widely used in poultry, In the feedstuff of the animals such as piglet, beef cattle, milch cow, sheep.Feeder acidulant can improve daily ration palatability, increases Piglet feed intake, early-weaned piglets uses Fumaric acid to make rate of body weight gain improve 5.1%, and feedstuff-meat ratio reduces 6.5%.Conventional acidulant have the organic acid such as citric acid, Fumaric acid, lactic acid, dystopy acid and formic acid, third The mineral acids such as acid, hydrochloric acid, phosphoric acid.Different acidulant is respectively arranged with its feature, but use the most extensively, effect relatively Good is citric acid, Fumaric acid and Compound-acid.Fumaric acid has broad-spectrum sterilization and bacteriostatic activity, as Feedstuff adds the Fumaric acid of 0.2%~0.4% concentration, staphylococcus and streptococcus can be killed;0.4% can Kill escherichia coli;Toxigenic fungi is had by 2% concentrations above to be killed and inhibitory action.
At present, domestic feeder acidulant be mostly fermentation liquor neutralization, acidolysis, decolouring, from handing over and crystallization etc. The compound product of the food grade organic acid after refinement treatment, its organic acid is single, and tart flavour is pained.Food stage has Machine acid produce be intended to through neutralizations, acidolysis, decolouring, from friendship or crystallization production process.Neutralizing with acidolysis is handle Organic acid is isolated and purified out, and both can produce substantial amounts of waste water and waste liquid, pollutes quite serious, consumption energy consumption Water, cost is high.Decolouring is to slough the material such as vitamin and pigment or nutrition, is to slough mineral element from friendship, Such as calcium, phosphorus, magnesium, sodium, potassium, chlorine, sulfur, ferrum, copper, zinc, manganese, iodine, cobalt, selenium etc..Decolouring and from Contacts, toward all producing substantial amounts of waste water, waste liquid, pollute weight, and cost is high, and tart flavour can be caused pained. Therefore, feeding do not neutralize, not acidolysis, nondiscoloration, not from the original flavor product handed over will be the direction of future development. But, the most not yet find not neutralize, not acidolysis, nondiscoloration, directly multiple from the organic acid fermentation liquid handed over The feed product joined.Owing to the production skill that solid fermentation adsorptive liquid ferments is taked in the production of this patent organic acid Art, after fermentation without neutralizations, acidolysis, decolouring, from friendship and the refined raw production. art such as crystallization.Therefore, this patent It is essentially different with the production technology of food grade organic acid.
Probiotic bacteria (Probiotics) has another name called probiotics, probiotics, regulator, profit raw element, bacteria-promoting agent etc.. Nineteen ninety Sogaard proposes accurate probiotics concept: i.e. " Probiotics is to take in animal body internal reference Direct by strengthening the inhibitory action to intestinal toxic microbiologic population with having of intestinal microbial balance, or Person prevents disease by enhancing non-specific immunity function, and indirectly plays promotion growth of animal effect and carry The active microorganism culture of high feed conversion rate ", it is referred to as human body and the eighth-largest nutrient of animal.Test card Bright: probiotic bacteria can replace or reduce antibiotic, by consuming oxygen, secretion antibacterial peptide and lactic acid, suppression corruption Lose bacteria growing, prevent and treat piglet diarrhea;After using probiotic bacteria, molasses anti pathologic immunity power significantly improves.
Before this, applicant Liu Xuan instrument and Meng Long once declared patent " a kind of fruit acid feed additive Preparation method " " a kind of poultry acidifying of (number of patent application: CN201510424595.8) and applicant Preparation method with zymolysis feedstuff " (number of patent application: CN201510997145.8) elaboration molasses are new The preparation method of type fodder acidulant.But the preparation method of these two patents has following defects that
One, do not illustrate molasses and produce organic acid to produce the skill of composite organic acid acidulant containing sugar products Art technique, particularly molasses and the production organic acid containing sugar products liquid fermentation produce composite organic acid acidifying The technical matters of agent.Meanwhile, the zymocyte in these two patents lacks acetic acid, propanoic acid and butyric fermentation strain.
Two, be required to before the fermentation of starch-containing series products to add α-amylase, saccharifying enzyme enzymolysis produce maltodextrin and Glucose, technique is relative complex, and cost is higher.And molasses and containing sugar products ferment before need not add α-amylase, saccharifying enzyme enzymolysis.Molasses and the fermentation production of organic acid technique Han sugar products are simpler, cost Ratio is less expensive.
Three, two patents all directly crystallize when containing pigment with containing ion without reference to citric acid Technique and solid fermented material absorption mother solution technique.
Four, two patents are not the most directly by citric acid fermentation broth, lactic fermentation liquid and other organic acid fermentation Liquid and zymolysis slag dregs of rice culture medium wet basis are mixed with acidifying and the Technology of zymolysis feedstuff.
Five, two last compounding process of patent are all not involved with add Chinese herbal medicine and vitamin technology.
Therefore, utilize molasses and produce organic acid containing sugar products liquid fermentation, and being adsorbed in fermentation residue In dregs of rice feedstuff, it it is the product thinking of an innovation.
Summary of the invention
It is contemplated that overcome above-mentioned through, functional activity nutritional labeling pained from the fodder acidulant handed over of decolouring Lack and containing the deficiency such as a large amount of non-nutritive carrier white carbons, it is provided that a kind of rich in lactic acid, citric acid, little Peptide, oligosaccharide, probiotic bacteria, other organic acid, protein, crude fibre, crude fat, vitamin and mineral The nutritional labelings such as element, have diarrhea, good palatability, amino acid balance, nutrition high, easy to digest and anti- The molasses saccharide acidifying of the features such as trophic factors content is low and zymolysis feedstuff.
The technical solution used in the present invention is as follows:
The acidifying of a kind of molasses saccharide and the preparation method of zymolysis feedstuff, comprise the following steps:
1., sugar slag wet basis, citric acid fermentation broth and the preparation of citric acid acid sludge wet basis:
By molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, and after high temperature sterilize cooling, accesses and produces citric acid After strain fermentation, then prepare citric acid acid sludge wet basis and citric acid fermentation broth through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access and produce lemon After lemon acid strain fermentation, then prepare citric acid acid sludge wet basis and citric acid fermentation broth through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, after slag sugar liquid accesses product citric acid strain fermentation, then prepare citric acid through solid-liquid separation Slag wet basis and citric acid fermentation broth;
2., sugar slag wet basis, lactic fermentation liquid and the preparation of lactic acid acid sludge wet basis:
By molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, and high temperature sterilize cools down, and accesses lactic acid producing strain, Under conditions of stream hydro-oxidation calcium solution continuously after fermentation, add dilute sulfuric acid and be acidified, divide through solid-liquid From prepared lactic acid acid sludge wet basis, calcium sulfate and lactic fermentation liquid;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access galactopoiesis Acid strain, under conditions of continuously stream hydro-oxidation calcium solution after fermentation, adds after dilute sulfuric acid is acidified, Lactic acid acid sludge wet basis, calcium sulfate and lactic fermentation liquid is prepared through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, without slag sugar liquid accesses lactic acid producing strain, send out under conditions of stream hydro-oxidation calcium solution continuously After ferment, add dilute sulfuric acid and be acidified, prepare lactic acid acid sludge wet basis, calcium sulfate and lactic acid through solid-liquid separation Fermentation liquid;
3., sugar slag wet basis, other organic acid fermentation liquid and the preparation of other organic acid acid sludge wet basis:
Or by molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, high temperature sterilize cools down, and other have to access product Machine acid strain, under conditions of stream hydro-oxidation calcium solution continuously after fermentation, adds dilute sulfuric acid and is acidified, Other organic acid acid sludge wet basis, calcium sulfate and other organic acid fermentation liquid is prepared through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access and produce it His organic acid strain, under conditions of stream hydro-oxidation calcium solution continuously after fermentation, adds dilute sulfuric acid and carries out Acidifying, prepares other organic acid acid sludge wet basis, calcium sulfate and other organic acid fermentation liquid through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, produce other organic acid strains without slag sugar liquid accesses, at the bar flowing hydro-oxidation calcium solution continuously After part bottom fermentation, add dilute sulfuric acid and be acidified, through solid-liquid separation prepare other organic acid acid sludge wet basis, Calcium sulfate and other organic acid fermentation liquid;
Wherein, other organic acid refers to the organic acid in addition to lactic acid, citric acid;
4., the preparation of fermentation source organic acid concentrated solution:
After one or more mixing in lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid Evaporation and concentration obtains fermentation source organic acid concentrated solution;
Or the one in lactic acid concentrated solution, citric acid concentrated solution or citric acid mother liquor and other organic acid concentrated solution Or multiple it is mixed to get fermentation source organic acid concentrated solution;
Wherein, lactic acid is sent out concentrated solution and lactic fermentation liquid evaporation and concentration is obtained;It is by lemon that citric acid concentrates Lemon acid fermentation liquid evaporation and concentration obtains;Citric acid mother liquor is through evaporation and concentration, low by citric acid fermentation broth Obtaining mother solution after Citric acid crystal and Fructus Citri Limoniae crystallization after temperature crystallization, after Fructus Citri Limoniae crystallization, mother solution returns again to evaporation and concentration Obtain citric acid mother liquor;Other organic acid fermentation liquid evaporation and concentration are obtained by other organic acid concentrated solutions;
5., the preparation of zymolysis slag dregs of rice culture medium wet basis:
By in sugar slag wet basis, citric acid acid sludge wet basis, lactic acid acid sludge wet basis, other organic acid acid sludge wet basis One or more mix with composite bacteria liquid, compound enzyme after enzymatic hydrolysis and fermentation, prepare zymolysis slag dregs of rice culture medium wet basis;
Or by sugar slag wet basis, citric acid acid sludge wet basis, lactic acid acid sludge wet basis, other organic acid acid sludge wet basis One or more mix with feedstuff, clean water, composite bacteria liquid, compound enzyme after enzymatic hydrolysis and fermentation, system Obtain zymolysis slag dregs of rice culture medium wet basis;
Or by enzymatic hydrolysis and fermentation after feedstuff, clean water, composite bacteria liquid, compound enzyme mixing, prepare zymolysis Slag dregs of rice culture medium wet basis;
6., the acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
By one or more in lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid and feedstuff The acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or mixed after being respectively dried is obtained after raw material mixing Conjunction obtains the acidifying of molasses saccharide and zymolysis feedstuff butt;
Or after being mixed by fermentation source organic acid concentrated solution, obtain the acidifying of molasses saccharide and zymolysis feedstuff with feedstuff Wet basis, or mixing post-drying or be mixed to get the acidifying of molasses saccharide and zymolysis feedstuff butt after being respectively dried;
Or fermentation source organic acid concentrated solution and zymolysis slag dregs of rice culture medium wet basis are mixed to get the acidifying of molasses saccharide and Zymolysis feedstuff wet basis, or mixing post-drying or be mixed and made into the acidifying of molasses saccharide and zymolysis feedstuff after being respectively dried Butt;
Or by one or more in fermentation source organic acid concentrated solution, citric acid mother liquor and Citric acid crystal and ferment Solve slag dregs of rice culture medium wet basis and be mixed to get the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mix post-drying or divide Not Hong Gan after be mixed to get molasses saccharide acidifying and zymolysis feedstuff butt;
Or by commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, acylate, sugar molasse sugar One or more in slurry, liquid fat, Chinese herbal medicine and vitamin and fermentation source organic acid concentrated solution, Fructus Citri Limoniae Acid mother liquor, other organic acid concentrated solution, zymolysis slag dregs of rice culture medium wet basis are mixed to get the acidifying of molasses saccharide and ferment Solve feedstuff wet basis, or mixing post-drying or be mixed to get the acidifying of molasses saccharide after being respectively dried and zymolysis feedstuff is done Base.
Preferably, the acidifying of a kind of molasses saccharide and the preparation method of zymolysis feedstuff, comprise the following steps:
1., sugar slag wet basis, citric acid fermentation broth and the preparation of citric acid acid sludge wet basis:
By molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, and after high temperature sterilize cooling, accesses and produces citric acid After strain fermentation, then prepare citric acid acid sludge wet basis and citric acid fermentation broth through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access and produce lemon After lemon acid strain fermentation, then prepare citric acid acid sludge wet basis and citric acid fermentation broth through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, after slag sugar liquid accesses product citric acid strain fermentation, then prepare citric acid through solid-liquid separation Slag wet basis and citric acid fermentation broth;
2., sugar slag wet basis, lactic fermentation liquid and the preparation of lactic acid acid sludge wet basis:
By molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, and high temperature sterilize cools down, and accesses lactic acid producing strain, Under conditions of stream hydro-oxidation calcium solution continuously after fermentation, add dilute sulfuric acid and be acidified, divide through solid-liquid From prepared lactic acid acid sludge wet basis, calcium sulfate and lactic fermentation liquid;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access galactopoiesis Acid strain, under conditions of continuously stream hydro-oxidation calcium solution after fermentation, adds after dilute sulfuric acid is acidified, Lactic acid acid sludge wet basis, calcium sulfate and lactic fermentation liquid is prepared through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, without slag sugar liquid accesses lactic acid producing strain, send out under conditions of stream hydro-oxidation calcium solution continuously After ferment, add dilute sulfuric acid and be acidified, prepare lactic acid acid sludge wet basis, calcium sulfate and lactic acid through solid-liquid separation Fermentation liquid;
3., the preparation of fermentation source organic acid concentrated solution:
After one or more mixing in lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid Evaporation and concentration obtains fermentation source organic acid concentrated solution;
Or one or more in lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid are the denseest Fermentation source organic acid concentrated solution is obtained after contracting;
Or one or more in lactic acid concentrated solution, citric acid concentrated solution or citric acid mother liquor are mixed to get fermentation Source organic acid concentrated solution;
Wherein, lactic acid is sent out concentrated solution and lactic fermentation liquid evaporation and concentration is obtained;It is by lemon that citric acid concentrates Lemon acid fermentation liquid evaporation and concentration obtains;Citric acid mother liquor is through evaporation and concentration, low by citric acid fermentation broth Obtaining mother solution after Citric acid crystal and Fructus Citri Limoniae crystallization after temperature crystallization, after Fructus Citri Limoniae crystallization, mother solution returns again to evaporation and concentration Obtain citric acid mother liquor;
4., the preparation of zymolysis slag dregs of rice culture medium wet basis:
By in sugar slag wet basis, citric acid acid sludge wet basis, lactic acid acid sludge wet basis, other organic acid acid sludge wet basis One or more mix with composite bacteria liquid, compound enzyme after enzymatic hydrolysis and fermentation, prepare zymolysis slag dregs of rice culture medium wet basis;
Or by sugar slag wet basis, citric acid acid sludge wet basis, lactic acid acid sludge wet basis, other organic acid acid sludge wet basis One or more mix with feedstuff, clean water, composite bacteria liquid, compound enzyme after enzymatic hydrolysis and fermentation, system Obtain zymolysis slag dregs of rice culture medium wet basis;
Or by enzymatic hydrolysis and fermentation after feedstuff, clean water, composite bacteria liquid, compound enzyme mixing, prepare zymolysis Slag dregs of rice culture medium wet basis;
5., the acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
Obtain after lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid are mixed with feedstuff The acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or be mixed to get molasses saccharide after being respectively dried Acidifying and zymolysis feedstuff butt;
Or after fermentation source organic acid concentrated solution, citric acid mother liquor and Citric acid crystal are mixed with feedstuff To the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or be mixed to get molasses sugar after being respectively dried Class acidifying and zymolysis feedstuff butt;
Or by wet with zymolysis slag dregs of rice culture medium to lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid Base is mixed to get the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or mixing system after being respectively dried The acidifying of saccharogenesis Mel class and zymolysis feedstuff butt;
Or by wet with zymolysis slag dregs of rice culture medium to fermentation source organic acid concentrated solution, citric acid mother liquor and Citric acid crystal Base is mixed to get the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or mix after being respectively dried To the acidifying of molasses saccharide and zymolysis feedstuff butt;
Or by commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, acylate, sugar molasse sugar One or more in slurry, liquid fat, Chinese herbal medicine and vitamin and fermentation source organic acid concentrated solution, zymolysis Slag dregs of rice culture medium wet basis is mixed to get the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixes post-drying or difference The acidifying of molasses saccharide and zymolysis feedstuff butt it is mixed to get after drying.
Further, the one or many of molasses series products during described molasses are National agricultural portion feedstuff catalogue Kind, for one or more in Sugar cane molasses, beet molasses, soy molasses;
Described is one or more containing sugar products in National agricultural portion feedstuff catalogue containing sugar products, For one or more in Caulis Sacchari sinensis, Radix Betae, fruit, marc;
Other organic acid described be in National agricultural portion feed additive catalogue organic acid one or more, for Fumaric acid, malic acid, a-ketoglutaric acid, succinic acid, tartaric acid, acetic acid, propanoic acid, butanoic acid, ascorbic acid, One or more in salicylic acid, gibberellin, kojic acid, gluconic acid, methylene succinic acid;
Described feedstuff be in National agricultural portion feedstuff catalogue feedstuff one or more or on State feedstuff dilated product in one or more, for bean cake, cottonseed meal, rapeseed meal, Semen arachidis hypogaeae dregs, Fish flour, blood powder, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, wheat gluten flour, jade Rice gluten powder, rice protein powder, DDGS, Semen sojae atricolor, Semen Pisi sativi, Semen Phaseoli, Semen Maydis, Oryza glutinosa, crack rice, Semen Tritici aestivi, Wheat bran, Testa oryzae, flour, Herba bromi japonici, Fructus Hordei Vulgaris, Radix Ipomoeae, soybean protein isolate, soybean protein concentrate, Semen sojae atricolor Skin, skin of Semen Maydis, pasture powder, Chinese herbal medicine, fruit and vegetable, sugar molasse syrup and food processing industry side-product, Or above-mentioned feedstuff dilated product in one or more;
Described composite bacteria liquid be in National agricultural portion feedstuff catalogue microniological proudcts one or more, For lactobacillus suspension, yeast liquid, bacillus subtilis bacterium solution, Bacillus licheniformis liquid, bacillus bifidus liquid, rice Aspergillosis liquid, Brevibacterium acetylicum liquid, clostridium tyrobutyricum liquid, more than two kinds of propionibacterium liquid;
Described compound enzyme is commercial protein enzyme, xylanase, glucanase, mannonase pectase In more than two kinds;
Described commercially available feeding non-fermented source organic acid is in National agricultural portion feed additive catalogue there to be non-fermented One or more of machine acid, for the one in formic acid, benzoic acid, tartaric acid, fumaric acid, sorbic acid or many Kind;
Described commercially available feeding mineral acid is the one or many of mineral acid in National agricultural portion feed additive catalogue Kind, for one or more in phosphoric acid, hydrochloric acid, sulphuric acid;
Described acylate be in National agricultural portion feed additive catalogue acylate one or more, for Calcium formate, sodium propionate, calcium propionate, sodium butyrate, sodium benzoate, sodium sorbate, potassium sorbate, citric acid One or more in potassium, sodium citrate, calcium citrate;
Described sugar molasse syrup is the one or many of sugar molasse syrup in National agricultural portion feedstuff catalogue Kind, for sucrose, glucose, fructose, glucose syrup, maltodextrin slurry/powder, high fructose syrup/powder, maltose One or more in slurry/powder, oligosaccharide syrup/powder, Sugar cane molasses, beet molasses;
Described liquid fat be in National agricultural portion feedstuff catalogue liquid fat one or more, for greatly Oleum Glycines and oil emulsion, Semen Maydis oil and oil emulsion thereof, Petiolus Trachycarpi oil and oil emulsion thereof, Testa oryzae oil and oil emulsion thereof, Oleum Cocois and oil emulsion, fish oil and animal oil and oil emulsion, Oleum Arachidis hypogaeae semen and oil emulsion thereof, Oleum Brassicae campestris and One or more in its oil emulsion, Oleum Camelliae and oil emulsion thereof and plants essential oil and extract thereof;
Described Chinese herbal medicine be in National agricultural portion Chinese herbal feed raw material and Chinese herbal veterinary medicine catalogue Chinese herbal medicine and One or more in extract, for the Radix Astragali and extract, the Cortex Eucommiae and extract thereof, Flos Lonicerae and carry Take one or more in thing, Fructus Crataegi and extract thereof, Pericarpium Citri Reticulatae and extract thereof, Flos Carthami and extract thereof;
Described vitamin is fatsoluble vitamin, vitamin B and vitamin C,.
Preferably, one or both during described molasses are Sugar cane molasses, beet molasses;
Described is one or both in Caulis Sacchari sinensis, Radix Betae containing sugar products;
Other organic acid fermentation liquid described is a kind of fermentation liquid or many in fumaric acid, acetic acid, propanoic acid, butanoic acid Plant fermentation liquid;
Described feedstuff is bean cake, Zein powder, DDGS, Testa oryzae, pasture powder, Chinese herbal medicine, or Above-mentioned feedstuff dilated product in one or more;
Described composite bacteria liquid be lactobacillus suspension, yeast liquid, more than two kinds of bacillus subtilis bacterium solution;
Described compound enzyme is commercial protein enzyme, xylanase, glucanase, mannonase pectase In more than two kinds;
Described commercially available feeding non-fermented source organic acid is one or more in formic acid, benzoic acid, fumaric acid;
Described commercially available feeding mineral acid is phosphoric acid;
Described acylate is one or both in calcium formate, sodium butyrate;
Described sugar molasse syrup be sucrose, glucose one or many in slurry, Sugar cane molasses, beet molasses Kind;
Described liquid fat is soybean oil and oil emulsion, Semen Maydis oil and oil emulsion thereof, Petiolus Trachycarpi oil and emulsifying thereof In oil, Testa oryzae oil and oil emulsion, Oleum Cocois and oil emulsion thereof, fish oil and animal oil and oil emulsion thereof one Plant or multiple;
Described Chinese herbal medicine is the Radix Astragali and extract thereof;
Described vitamin is vitamin C.
Further, the preparation condition of fermentation source organic acid concentrated solution is as follows:
(1) when preparing citric acid fermentation broth: product citric acid strain fermentation temperature: 5~50 DEG C;Fermentation pH: Natural;Fermented type: aerobic fermentation;Fermentation time: 24-96 hour;
(2) when, preparing lactic fermentation liquid: lactic acid producing strain fermentation temperature: 10~50 DEG C;Fermentation pH:4~ 10;Fermented type: anaerobic fermentation;Fermentation time: 24-144 hour;
(3), the weight ratio of citric acid fermentation broth, lactic fermentation liquid and other organic acid fermentation liquid be 100:0~ 500:0~300.
Preferably, the preparation condition of fermentation source organic acid concentrated solution is as follows:
(1) when, preparing citric acid fermentation broth: product citric acid waste residue strain fermentation temperature: 30~36 DEG C; Fermentation pH: natural;Fermented type: aerobic fermentation;Fermentation time: 48-60 hour;
(2) when, preparing lactic fermentation liquid: lactic acid producing strain fermentation temperature: 28~32 DEG C;Fermentation pH:6~ 7;Fermented type: anaerobic fermentation;Fermentation time: 36-48 hour;
(3), the weight ratio of citric acid fermentation broth, lactic fermentation liquid and other organic acid fermentation liquid be 100:30~ 60:20~30.
Further, the preparation condition of zymolysis slag dregs of rice culture medium wet basis is as follows:
(1), sugar slag wet basis, citric acid acid sludge wet basis, other organic acid acid sludge wet basis and feedstuff, dry Water purification, composite bacteria liquid, the weight ratio of compound enzyme are 100:0~300:0~300:0~500:0~1000: 0.1~100:0.00001~20;
(2), the lactobacillus suspension in composite bacteria liquid, yeast liquid, bacillus subtilis bacterium solution, lichens spore Bacillus liquid, bacillus bifidus liquid, aspergillus oryzae liquid, Brevibacterium acetylicum liquid, clostridium tyrobutyricum liquid, propionibacterium liquid Number of viable than for 100:0~300:0~300:0~100:0~100:0~100:0~50:0~ 50:0~50;
(3), the commercial protein enzyme in compound enzyme: xylanase: glucanase: mannase: pectin The weight ratio of enzyme is 100:0~500:0~500:0~500:0~500;
(4), enzymatic hydrolysis and fermentation temperature: 5~50 DEG C, enzymatic hydrolysis and fermentation initiates pH:3~8, and first aerobic enzymolysis is sent out Anaerase hydrolysis and fermentation 24-168 hour after ferment.
Preferably, the preparation condition of zymolysis slag dregs of rice culture medium wet basis is as follows:
(1), sugar slag wet basis, citric acid acid sludge wet basis, organic acid acid sludge wet basis and feedstuff, clean water, Composite bacteria liquid, the weight ratio of compound enzyme are 100:5~50:2~20:50~400:10~50:5~10: 0.003~3;
(2), the lactic acid bacteria culturers liquid in composite bacteria liquid: yeast seeds liquid: Bacillus licheniformis strain The number of viable of liquid is than for 100:2~20:1~10;
(3), the commercial protein enzyme in compound enzyme: xylanase: glucanase: mannase: pectin The weight ratio of enzyme is 100:30~50:30~50:30~50:10~30;
(4), enzymatic hydrolysis and fermentation temperature: 28~36 DEG C, enzymatic hydrolysis and fermentation initiates pH:6~8, first aerobic enzymolysis Anaerase hydrolysis and fermentation 60-72 hour after fermentation.
Further, fermentation source organic acid concentrated solution, zymolysis slag dregs of rice culture medium wet basis and commercially available feeding non-fermented Source organic acid, commercially available feeding mineral acid, acylate, sugar molasse syrup, liquid fat, Chinese herbal medicine and The weight ratio of vitamin is 100:0~1000:0~200:0~200:0~200:0~300:0~300: 0~50:0~50.
Preferably, fermentation source organic acid concentrated solution, zymolysis slag dregs of rice culture medium wet basis and commercially available feeding non-fermented source Organic acid, commercially available feeding mineral acid, acylate, sugar molasse syrup, liquid fat, Chinese herbal medicine and dimension The weight ratio of raw element is 100:50~500:10~30:5~10:5~10:20~60:2~10:0~5: 0~5.
The beneficial effects of the present invention is: the acidifying of molasses saccharide and zymolysis feedstuff prepared by the present invention, rich in breast Acid, citric acid, little peptide, oligosaccharide, probiotic bacteria, other organic acid, protein, crude fibre, crude fat, The nutritional labeling such as vitamin and mineral element, have diarrhea, good palatability, amino acid balance, nutrition high, The features such as easy to digest and antinutritional factor content is low.
Detailed description of the invention
Embodiment 1
The acidifying of a kind of molasses saccharide and the preparation method of zymolysis feedstuff, comprise the following steps:
(1), citric acid fermentation broth and the preparation of citric acid acid sludge wet basis:
By 100kg molasses, after sloughing mineral ion from handing over deferrization, add 200kg water and size mixing after pH, make Obtain 300kg band slag liquid molasses.In 300kg band slag liquid molasses, after high temperature sterilize cooling, access and produce Fructus Citri Limoniae The aspergillus niger strain liquid 25kg of acid, 32-36 DEG C, fermentation pH naturally under the conditions of, carry out aerobic fermentation 60 hours, prepare the rough citric acid fermentation broth of 325kg.50kg citric acid acid sludge wet basis is prepared through solid-liquid separation With 275kg citric acid fermentation broth.
(2), the preparation of citric acid concentrated solution:
After directly being concentrated by 275kg citric acid fermentation broth, prepare 90kg citric acid concentrated solution.
(3), the preparation of zymolysis slag dregs of rice culture medium wet basis:
In 50kg citric acid acid sludge wet basis, add 80kg bean cake, 12kg molasses, 50kg clean water, 10kg After the mixing of composite bacteria liquid, 0.06kg compound enzyme, under the conditions of temperature 30~32 DEG C and initial pH:6~8, After first aerobic, anaerobic fermentation is after 72 hours, prepares 198kg zymolysis slag dregs of rice culture medium wet basis.
(4) acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
After 90kg citric acid concentrated solution and 198kg zymolysis slag dregs of rice culture medium wet basis being mixed, it is directly produced 275kg The molasses saccharide of water content 50-60% is acidified and zymolysis feedstuff wet basis, or the acid of 120kg molasses saccharide is made in drying Change and zymolysis feedstuff butt.
Or by 90kg citric acid concentrated solution and 198kg zymolysis slag dregs of rice culture medium wet basis and 60kg fumaric acid solution, After the mixing of 50kg phosphoric acid, dry and make the acidifying of 230kg molasses saccharide and zymolysis feedstuff butt.
Embodiment 2
The acidifying of a kind of molasses saccharide and the preparation method of zymolysis feedstuff, comprise the following steps:
(1) preparation of the preparation of sugar slag wet basis, lactic fermentation liquid and lactic acid acid sludge wet basis:
100kg cane milling is crushed, adds after 200kg water sizes mixing, prepare 300kg band slag sucrose liquid.Warp Solid-liquid separation prepares 20kg sugar slag wet basis and 280kg without slag sucrose liquid.
At 280kg without in slag sucrose liquid, after high temperature sterilize cooling, access the aspergillus niger strain liquid producing citric acid 25kg, 32-36 DEG C, fermentation pH naturally under the conditions of, carry out aerobic fermentation 72 hours, prepare 300kg Rough citric acid fermentation broth.50kg citric acid acid sludge wet basis and 250kg citric acid fermentation is prepared through solid-liquid separation Liquid.
(2), the preparation of citric acid concentrated solution:
After directly being concentrated by 250kg citric acid fermentation broth, prepare 60kg citric acid concentrated solution.
(3), the preparation of zymolysis slag dregs of rice culture medium wet basis:
In 60kg citric acid acid sludge wet basis, add 50kg bean cake, 8kg molasses, 30kg clean water, 8kg After the mixing of composite bacteria liquid, 0.05kg compound enzyme, under the conditions of temperature 30~32 DEG C and initial pH:6~8, After first aerobic, anaerobic fermentation is after 72 hours, prepares 150kg zymolysis slag dregs of rice culture medium wet basis.
(4) acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
After 60kg citric acid concentrated solution and 150kg zymolysis slag dregs of rice culture medium wet basis being mixed, it is directly produced 215kg The molasses saccharide of water content 50-60% is acidified and zymolysis feedstuff wet basis, or the acid of 105kg molasses saccharide is made in drying Change and zymolysis feedstuff butt.
Or by 60kg citric acid concentrated solution, 150kg zymolysis slag dregs of rice culture medium wet basis, 50kg fumaric acid solution, After 30kg phosphoric acid and the mixing of 5kg vitamin C, the acidifying of 200kg molasses saccharide is made in drying and zymolysis feedstuff is done Base.
Embodiment 3
(1), citric acid acid sludge, citric acid fermentation broth and the preparation of citric acid acid sludge wet basis:
By 100kg molasses, after sloughing mineral ion from handing over deferrization, add 200kg water and size mixing after pH, make Obtain 300kg band slag liquid molasses.In 300kg band slag liquid molasses, after high temperature sterilize cooling, access and produce Fructus Citri Limoniae The aspergillus niger strain liquid 25kg of acid, 32-36 DEG C, fermentation pH naturally under the conditions of, carry out aerobic fermentation 60 hours, prepare the rough citric acid fermentation broth of 325kg.50kg citric acid acid sludge wet basis is prepared through solid-liquid separation With 275kg citric acid fermentation broth.
(2), the preparation of the preparation of sugar slag wet basis, lactic fermentation liquid and lactic acid acid sludge wet basis:
By 100kg molasses, after sloughing mineral ion from handing over deferrization, add 200kg water and size mixing after pH, make Obtain 300kg band slag liquid molasses.In 300kg band slag liquid molasses, after high temperature sterilize cooling, access lactic acid producing Lactic acid bacteria culturers liquid 10kg, at continuously stream hydro-oxidation calcium solution, temperature 42-45 DEG C, fermentation pH:7 Substantially constant under the conditions of, carry out anaerobic fermentation 48-60 hour, prepare the rough lactic fermentation liquid of 305kg.? Adding 40kg20% dilution heat of sulfuric acid in 305kg lactic fermentation liquid, acidifying generates calcium sulfate and lactic acid.Through solid-liquid Separate and prepare 15kg lactic acid acid sludge wet basis and calcium sulfate wet basis, 290kg lactic fermentation liquid.Wherein, dlactic acid Slag wet basis and calcium sulfate wet basis are abandoned.
(3), the preparation of organic acid concentrated solution:
Mix after again 275kg citric acid fermentation broth and 290kg lactic fermentation liquid being mixed after concentrating or concentrating respectively Close, prepare 150kg organic acid concentrated solution.
(4), the preparation of zymolysis slag dregs of rice culture medium wet basis:
In 50kg citric acid acid sludge wet basis and 15kg lactic acid acid sludge wet basis, add 60kg bean cake, 10kg fish After the mixing of powder, 5kg molasses, 10kg composite bacteria liquid, 0.02kg compound enzyme, temperature 30~40 DEG C and initial Under the conditions of pH:6~8, after first aerobic, anaerobic fermentation is after 84 hours, prepares 125kg zymolysis slag dregs of rice culture medium Wet basis.
(5), the acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
After 150kg organic acid concentrated solution and 125kg zymolysis slag dregs of rice culture medium wet basis being mixed, drying is made The acidifying of 275kg molasses saccharide and zymolysis feedstuff.
Or by 150kg organic acid concentrated solution, 125kg zymolysis slag dregs of rice culture medium wet basis, 50kg fumaric acid solution, After 30kg phosphoric acid and the mixing of 15kg Radix Astragali extract, dry and make the acidifying of 210kg molasses saccharide and zymolysis feedstuff.
Embodiment 4
The acidifying of a kind of molasses saccharide and the preparation method of zymolysis feedstuff, comprise the following steps:
(1), citric acid fermentation broth and the preparation of citric acid acid sludge wet basis:
By 100kg molasses, after sloughing mineral ion from handing over deferrization, add 200kg water and size mixing after pH, make Obtain 300kg band slag liquid molasses.In 300kg band slag liquid molasses, after high temperature sterilize cooling, access and produce Fructus Citri Limoniae The aspergillus niger strain liquid 25kg of acid, 32-36 DEG C, fermentation pH naturally under the conditions of, carry out aerobic fermentation 60 hours, prepare the rough citric acid fermentation broth of 325kg.50kg citric acid acid sludge wet basis is prepared through solid-liquid separation With 275kg citric acid fermentation broth.
(2), the preparation of citric acid concentrated mother liquor:
After first directly being concentrated by 275kg citric acid fermentation broth, prepare 90kg citric acid concentrated solution.After by 90kg After citric acid concentrated solution low temperature crystallization, prepare mother solution after 30kg Citric acid crystal and the crystallization of 60 citric acids, After being crystallized by 60kg citric acid, after the crystallization of mother solution citric acid, mother solution returns again to evaporation and concentration and produces 35kg lemon again Lemon acid concentrated mother liquor;
(3), the preparation of zymolysis slag dregs of rice culture medium wet basis:
In 50kg citric acid acid sludge wet basis, add 80kg bean cake, 12kg molasses, 50kg clean water, 10kg After the mixing of composite bacteria liquid, 0.06kg compound enzyme, under the conditions of temperature 30~32 DEG C and initial pH:6~8, After first aerobic, anaerobic fermentation is after 72 hours, prepares 198kg zymolysis slag dregs of rice culture medium wet basis.
(4) acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
30kg Citric acid crystal, 35kg citric acid concentrated mother liquor and 198kg zymolysis slag dregs of rice culture medium wet basis are mixed After conjunction, it is directly produced the acidifying of molasses saccharide and the zymolysis feedstuff wet basis of 245-250kg water content 50-60%, or The acidifying of 120kg molasses saccharide and zymolysis feedstuff butt are made in drying.
Or by 30kg Citric acid crystal, 35kg citric acid concentrated mother liquor and 198kg zymolysis slag dregs of rice culture medium wet basis After 60kg fumaric acid solution, the mixing of 50kg phosphoric acid, it is directly produced 373-375kg water content 50-60% The acidifying of molasses saccharide and zymolysis feedstuff wet basis, or the acidifying of 230kg molasses saccharide is made in drying and zymolysis feedstuff is done Base.
Embodiment 5
The acidifying of a kind of molasses saccharide and the preparation method of zymolysis feedstuff, comprise the following steps:
(1), citric acid fermentation broth and the preparation of citric acid acid sludge wet basis:
By 100kg molasses, after sloughing mineral ion from handing over deferrization, add 200kg water and size mixing after pH, make Obtain 300kg band slag liquid molasses.In 300kg band slag liquid molasses, after high temperature sterilize cooling, access and produce Fructus Citri Limoniae The aspergillus niger strain liquid 25kg of acid, 32-36 DEG C, fermentation pH naturally under the conditions of, carry out aerobic fermentation 60 hours, prepare the rough citric acid fermentation broth of 325kg.50kg citric acid acid sludge wet basis is prepared through solid-liquid separation With 275kg citric acid fermentation broth.
(2), the preparation of zymolysis slag dregs of rice culture medium wet basis:
In 50kg citric acid acid sludge wet basis, add 80kg bean cake, 12kg molasses, 50kg clean water, 10kg After the mixing of composite bacteria liquid, 0.06kg compound enzyme, under the conditions of temperature 30~32 DEG C and initial pH:6~8, After first aerobic, anaerobic fermentation is after 72 hours, prepares 198kg zymolysis slag dregs of rice culture medium wet basis.
(3) acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
30kg Citric acid crystal, 275kg citric acid fermentation broth and 198kg zymolysis slag dregs of rice culture medium wet basis are mixed After conjunction, it is directly produced the acidifying of molasses saccharide and the zymolysis feedstuff wet basis of 498-501kg water content 50-60%, or The acidifying of 120kg molasses saccharide and zymolysis feedstuff butt are made in drying.
Or by 30kg Citric acid crystal, 275kg citric acid fermentation broth and 198kg zymolysis slag dregs of rice culture medium wet basis After 60kg fumaric acid solution, the mixing of 50kg phosphoric acid, it is directly produced 648-655kg water content 50-60% The acidifying of molasses saccharide and zymolysis feedstuff wet basis, or the acidifying of 230kg molasses saccharide is made in drying and zymolysis feedstuff is done Base.
One, main active substances content in the molasses saccharide acidifying zymolysis feedstuff of embodiment 1 preparation.Refer to table 1 below.
The molasses saccharide acidifying zymolysis feed product Middle nutrition content of material of table 1, embodiment 1 preparation
Project Index Project Index Project Index
Color and luster Yellowish-brown or light yellow Citric acid/% ≥12.0 Fructus Citri Limoniae calcium/% ≥0.5
Moisture/% ≤12.0 Pfansteihl/% ≥2.0 Total sugar/% ≥1.5
Crude protein/% ≥8.0 Fumaric acid/% - Oligosaccharide/% ≥1.0
Little peptide/% ≥1.0 Acetic acid/% - Total amount of probiotics cfu/g ≥0.03×106
Crude fat/% ≥1.0 Total organic acids/% ≥22.0 Water-soluble substances ≥30.0
Two, embodiment 1 preparation molasses saccharide acidifying zymolysis feedstuff feed ablactational baby pig effect.
Daily ration adds the commercially available acidulant of 5kg/ ton, 10kg/ ton, 20kg/ ton and the acidifying of 30kg/ ton molasses saccharide After zymolysis feedstuff, the daily gain of ablactational baby pig improves 9.71%, 7.30%, 9.62% and than matched group respectively 13.61%, diarrhea rate reduces by 82.66%, 81.41%, 83.76% and 97.26% than matched group respectively, searches for food Amount improves 4.45%, 6.57%, 3.62% and 5.83% than matched group respectively, and feedstuff-meat ratio drops than matched group respectively Low by 3.42%, 6.85%, 6.85% and 6.85%.Refer to table 2.
The acidifying zymolysis feedstuff impact on Production Performance of Weaning Pigs of table 2. molasses saccharide
Three, embodiment 1 preparation molasses saccharide acidifying zymolysis feedstuff feed broiler chicken effect.
After daily ration adds 5kg/ ton, 10kg/ ton and 15kg/ ton molasses saccharide acidifying zymolysis feedstuff, broiler chicken Daily gain improves 8.52%, 11.42% and 12.56% than matched group respectively, and feed intake improves than matched group respectively 6.52%, 8.48% and 11.52%, feedstuff-meat ratio reduces by 4.08%, 5.10% and 4.59% than matched group respectively; The average egg weight of laying hen improves 2.03%, 3.15% and 4.20% than matched group respectively, and laying rate is respectively than comparison Group raising 4.09%, 5.46% and 5.84%, feedstuff-egg ratio reduces by 3.47%, 4.95% and 4.95 than matched group respectively. Refer to table 3.
Table 3. molasses saccharide acidifying zymolysis feedstuff is on broiler and the impact of performance in layers
Four, embodiment 1 preparation molasses saccharide acidifying zymolysis feedstuff feed U.S.'s channel catfish Hui fish effect.
After daily ration adds 10kg/ ton, 20kg/ ton and 30kg/ ton molasses saccharide acidifying zymolysis feedstuff, and compare Group is compared the weightening finish of U.S.'s channel catfish Hui fish moon and is respectively increased 22.45%, 27.26% and 31.39%, and food ration is divided You can well imagine the highest by 8.21%, 6.52% and 13.82%, feed coefficient reduces by 11.63%, 16.28% and 13.37% respectively. Refer to table 4.
The acidifying zymolysis feedstuff impact on U.S.'s channel catfish Hui fish growth performance of table 4. molasses saccharide
Project Matched group 10kg/ ton 20kg/ ton 30kg/ ton
Just counterpoise/g 198.02 198.42 196.86 194.85
End counterpoise/g 320.22 348.05 352.33 355.41
Moon weightening finish/g 122.20 149.63 155.47 160.56
Food ration/g 210.18 227.44 223.88 239.23
Feed coefficient/% 1.72 1.52 1.44 1.49
Above content is only citing made for the present invention and explanation, affiliated those skilled in the art Described specific embodiment is made various amendment or supplements or use similar mode to substitute, as long as Without departing from invention design or surmount scope defined in the claims, the guarantor of the present invention all should be belonged to Protect scope.

Claims (10)

1. a molasses saccharide acidifying and the preparation method of zymolysis feedstuff, it is characterised in that comprise the following steps:
1., sugar slag wet basis, citric acid fermentation broth and the preparation of citric acid acid sludge wet basis:
By molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, and after high temperature sterilize cooling, accesses and produces citric acid After strain fermentation, then prepare citric acid acid sludge wet basis and citric acid fermentation broth through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access and produce lemon After lemon acid strain fermentation, then prepare citric acid acid sludge wet basis and citric acid fermentation broth through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, after slag sugar liquid accesses product citric acid strain fermentation, then prepare citric acid through solid-liquid separation Slag wet basis and citric acid fermentation broth;
2., sugar slag wet basis, lactic fermentation liquid and the preparation of lactic acid acid sludge wet basis:
By molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, and high temperature sterilize cools down, and accesses lactic acid producing strain, Under conditions of stream hydro-oxidation calcium solution continuously after fermentation, add dilute sulfuric acid and be acidified, divide through solid-liquid From prepared lactic acid acid sludge wet basis, calcium sulfate and lactic fermentation liquid;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access galactopoiesis Acid strain, under conditions of continuously stream hydro-oxidation calcium solution after fermentation, adds after dilute sulfuric acid is acidified, Lactic acid acid sludge wet basis, calcium sulfate and lactic fermentation liquid is prepared through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, without slag sugar liquid accesses lactic acid producing strain, send out under conditions of stream hydro-oxidation calcium solution continuously After ferment, add dilute sulfuric acid and be acidified, prepare lactic acid acid sludge wet basis, calcium sulfate and lactic acid through solid-liquid separation Fermentation liquid;
3., sugar slag wet basis, other organic acid fermentation liquid and the preparation of other organic acid acid sludge wet basis:
Or by molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, high temperature sterilize cools down, and other have to access product Machine acid strain, under conditions of stream hydro-oxidation calcium solution continuously after fermentation, adds dilute sulfuric acid and is acidified, Other organic acid acid sludge wet basis, calcium sulfate and other organic acid fermentation liquid is prepared through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access and produce it His organic acid strain, under conditions of stream hydro-oxidation calcium solution continuously after fermentation, adds dilute sulfuric acid and carries out Acidifying, prepares other organic acid acid sludge wet basis, calcium sulfate and other organic acid fermentation liquid through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, produce other organic acid strains without slag sugar liquid accesses, at the bar flowing hydro-oxidation calcium solution continuously After part bottom fermentation, add dilute sulfuric acid and be acidified, through solid-liquid separation prepare other organic acid acid sludge wet basis, Calcium sulfate and other organic acid fermentation liquid;
Wherein, other organic acid refers to the organic acid in addition to lactic acid, citric acid;
4., the preparation of fermentation source organic acid concentrated solution:
After one or more mixing in lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid Evaporation and concentration obtains fermentation source organic acid concentrated solution;
Or the one in lactic acid concentrated solution, citric acid concentrated solution or citric acid mother liquor and other organic acid concentrated solution Or multiple it is mixed to get fermentation source organic acid concentrated solution;
Wherein, lactic acid is sent out concentrated solution and lactic fermentation liquid evaporation and concentration is obtained;It is by lemon that citric acid concentrates Lemon acid fermentation liquid evaporation and concentration obtains;Citric acid mother liquor is through evaporation and concentration, low by citric acid fermentation broth Obtaining mother solution after Citric acid crystal and Fructus Citri Limoniae crystallization after temperature crystallization, after Fructus Citri Limoniae crystallization, mother solution returns again to evaporation and concentration Obtain citric acid mother liquor;Other organic acid fermentation liquid evaporation and concentration are obtained by other organic acid concentrated solutions;
5., the preparation of zymolysis slag dregs of rice culture medium wet basis:
By in sugar slag wet basis, citric acid acid sludge wet basis, lactic acid acid sludge wet basis, other organic acid acid sludge wet basis One or more mix with composite bacteria liquid, compound enzyme after enzymatic hydrolysis and fermentation, prepare zymolysis slag dregs of rice culture medium wet basis;
Or by sugar slag wet basis, citric acid acid sludge wet basis, lactic acid acid sludge wet basis, other organic acid acid sludge wet basis One or more mix with feedstuff, clean water, composite bacteria liquid, compound enzyme after enzymatic hydrolysis and fermentation, system Obtain zymolysis slag dregs of rice culture medium wet basis;
Or by enzymatic hydrolysis and fermentation after feedstuff, clean water, composite bacteria liquid, compound enzyme mixing, prepare zymolysis Slag dregs of rice culture medium wet basis;
6., the acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
By one or more in lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid and feedstuff The acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or mixed after being respectively dried is obtained after raw material mixing Conjunction obtains the acidifying of molasses saccharide and zymolysis feedstuff butt;
Or after being mixed by fermentation source organic acid concentrated solution, obtain the acidifying of molasses saccharide and zymolysis feedstuff with feedstuff Wet basis, or mixing post-drying or be mixed to get the acidifying of molasses saccharide and zymolysis feedstuff butt after being respectively dried;
Or fermentation source organic acid concentrated solution and zymolysis slag dregs of rice culture medium wet basis are mixed to get the acidifying of molasses saccharide and Zymolysis feedstuff wet basis, or mixing post-drying or be mixed and made into the acidifying of molasses saccharide and zymolysis feedstuff after being respectively dried Butt;
Or by one or more in fermentation source organic acid concentrated solution, citric acid mother liquor and Citric acid crystal and ferment Solve slag dregs of rice culture medium wet basis and be mixed to get the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mix post-drying or divide Not Hong Gan after be mixed to get molasses saccharide acidifying and zymolysis feedstuff butt;
Or by commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, acylate, sugar molasse sugar One or more in slurry, liquid fat, Chinese herbal medicine and vitamin and fermentation source organic acid concentrated solution, Fructus Citri Limoniae Acid mother liquor, other organic acid concentrated solution, zymolysis slag dregs of rice culture medium wet basis are mixed to get the acidifying of molasses saccharide and ferment Solve feedstuff wet basis, or mixing post-drying or be mixed to get the acidifying of molasses saccharide after being respectively dried and zymolysis feedstuff is done Base.
A kind of molasses saccharide the most according to claim 1 acidifying and the preparation method of zymolysis feedstuff, it is special Levy and be, comprise the following steps:
1., sugar slag wet basis, citric acid fermentation broth and the preparation of citric acid acid sludge wet basis:
By molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, and after high temperature sterilize cooling, accesses and produces citric acid After strain fermentation, then prepare citric acid acid sludge wet basis and citric acid fermentation broth through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access and produce lemon After lemon acid strain fermentation, then prepare citric acid acid sludge wet basis and citric acid fermentation broth through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, after slag sugar liquid accesses product citric acid strain fermentation, then prepare citric acid through solid-liquid separation Slag wet basis and citric acid fermentation broth;
2., sugar slag wet basis, lactic fermentation liquid and the preparation of lactic acid acid sludge wet basis:
By molasses from handing over deferrization, add water prepared liquid molasses of sizing mixing, and high temperature sterilize cools down, and accesses lactic acid producing strain, Under conditions of stream hydro-oxidation calcium solution continuously after fermentation, add dilute sulfuric acid and be acidified, divide through solid-liquid From prepared lactic acid acid sludge wet basis, calcium sulfate and lactic fermentation liquid;
Or after sugar-containing product is pulled an oar, sized mixing, prepare band slag sugar liquid, after high temperature sterilize cooling, access galactopoiesis Acid strain, under conditions of continuously stream hydro-oxidation calcium solution after fermentation, adds after dilute sulfuric acid is acidified, Lactic acid acid sludge wet basis, calcium sulfate and lactic fermentation liquid is prepared through solid-liquid separation;
Or after sugar-containing product is pulled an oar, sized mixing, solid-liquid separation prepares sugar slag wet basis and without slag sugar liquid;High temperature goes out After bacterium cooling, without slag sugar liquid accesses lactic acid producing strain, send out under conditions of stream hydro-oxidation calcium solution continuously After ferment, add dilute sulfuric acid and be acidified, prepare lactic acid acid sludge wet basis, calcium sulfate and lactic acid through solid-liquid separation Fermentation liquid;
3., the preparation of fermentation source organic acid concentrated solution:
After one or more mixing in lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid Evaporation and concentration obtains fermentation source organic acid concentrated solution;
Or one or more in lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid are the denseest Fermentation source organic acid concentrated solution is obtained after contracting;
Or one or more in lactic acid concentrated solution, citric acid concentrated solution or citric acid mother liquor are mixed to get fermentation Source organic acid concentrated solution;
Wherein, lactic acid is sent out concentrated solution and lactic fermentation liquid evaporation and concentration is obtained;It is by lemon that citric acid concentrates Lemon acid fermentation liquid evaporation and concentration obtains;Citric acid mother liquor is through evaporation and concentration, low by citric acid fermentation broth Obtaining mother solution after Citric acid crystal and Fructus Citri Limoniae crystallization after temperature crystallization, after Fructus Citri Limoniae crystallization, mother solution returns again to evaporation and concentration Obtain citric acid mother liquor;
4., the preparation of zymolysis slag dregs of rice culture medium wet basis:
By in sugar slag wet basis, citric acid acid sludge wet basis, lactic acid acid sludge wet basis, other organic acid acid sludge wet basis One or more mix with composite bacteria liquid, compound enzyme after enzymatic hydrolysis and fermentation, prepare zymolysis slag dregs of rice culture medium wet basis;
Or by sugar slag wet basis, citric acid acid sludge wet basis, lactic acid acid sludge wet basis, other organic acid acid sludge wet basis One or more mix with feedstuff, clean water, composite bacteria liquid, compound enzyme after enzymatic hydrolysis and fermentation, system Obtain zymolysis slag dregs of rice culture medium wet basis;
Or by enzymatic hydrolysis and fermentation after feedstuff, clean water, composite bacteria liquid, compound enzyme mixing, prepare zymolysis Slag dregs of rice culture medium wet basis;
5., the acidifying of molasses saccharide and the preparation of zymolysis feedstuff:
Obtain after lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid are mixed with feedstuff The acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or be mixed to get molasses saccharide after being respectively dried Acidifying and zymolysis feedstuff butt;
Or after fermentation source organic acid concentrated solution, citric acid mother liquor and Citric acid crystal are mixed with feedstuff To the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or be mixed to get molasses sugar after being respectively dried Class acidifying and zymolysis feedstuff butt;
Or by wet with zymolysis slag dregs of rice culture medium to lactic fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid Base is mixed to get the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or mixing system after being respectively dried The acidifying of saccharogenesis Mel class and zymolysis feedstuff butt;
Or by wet with zymolysis slag dregs of rice culture medium to fermentation source organic acid concentrated solution, citric acid mother liquor and Citric acid crystal Base is mixed to get the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixing post-drying or mix after being respectively dried To the acidifying of molasses saccharide and zymolysis feedstuff butt;
Or by commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, acylate, sugar molasse sugar One or more in slurry, liquid fat, Chinese herbal medicine and vitamin and fermentation source organic acid concentrated solution, zymolysis Slag dregs of rice culture medium wet basis is mixed to get the acidifying of molasses saccharide and zymolysis feedstuff wet basis, or mixes post-drying or difference The acidifying of molasses saccharide and zymolysis feedstuff butt it is mixed to get after drying.
A kind of molasses saccharide the most according to claim 1 acidifying and the preparation method of zymolysis feedstuff, it is special Levy and be,
Described molasses be in National agricultural portion feedstuff catalogue molasses series products one or more, for sucrose One or more in molasses, beet molasses, soy molasses;
Described is one or more containing sugar products in National agricultural portion feedstuff catalogue containing sugar products, For one or more in Caulis Sacchari sinensis, Radix Betae, fruit, marc;
Other organic acid described be in National agricultural portion feed additive catalogue organic acid one or more, for Fumaric acid, malic acid, a-ketoglutaric acid, succinic acid, tartaric acid, acetic acid, propanoic acid, butanoic acid, ascorbic acid, One or more in salicylic acid, gibberellin, kojic acid, gluconic acid, methylene succinic acid;
Described feedstuff be in National agricultural portion feedstuff catalogue feedstuff one or more or on State feedstuff dilated product in one or more, for bean cake, cottonseed meal, rapeseed meal, Semen arachidis hypogaeae dregs, Fish flour, blood powder, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, wheat gluten flour, jade Rice gluten powder, rice protein powder, DDGS, Semen sojae atricolor, Semen Pisi sativi, Semen Phaseoli, Semen Maydis, Oryza glutinosa, crack rice, Semen Tritici aestivi, Wheat bran, Testa oryzae, flour, Herba bromi japonici, Fructus Hordei Vulgaris, Radix Ipomoeae, soybean protein isolate, soybean protein concentrate, Semen sojae atricolor Skin, skin of Semen Maydis, pasture powder, Chinese herbal medicine, fruit and vegetable, sugar molasse syrup and food processing industry side-product, Or above-mentioned feedstuff dilated product in one or more;
Described composite bacteria liquid be in National agricultural portion feedstuff catalogue microniological proudcts one or more, For lactobacillus suspension, yeast liquid, bacillus subtilis bacterium solution, Bacillus licheniformis liquid, bacillus bifidus liquid, rice Aspergillosis liquid, Brevibacterium acetylicum liquid, clostridium tyrobutyricum liquid, more than two kinds of propionibacterium liquid;
Described compound enzyme is commercial protein enzyme, xylanase, glucanase, mannonase pectase In more than two kinds;
Described commercially available feeding non-fermented source organic acid is in National agricultural portion feed additive catalogue there to be non-fermented One or more of machine acid, for the one in formic acid, benzoic acid, tartaric acid, fumaric acid, sorbic acid or many Kind;
Described commercially available feeding mineral acid is the one or many of mineral acid in National agricultural portion feed additive catalogue Kind, for one or more in phosphoric acid, hydrochloric acid, sulphuric acid;
Described acylate be in National agricultural portion feed additive catalogue acylate one or more, for Calcium formate, sodium propionate, calcium propionate, sodium butyrate, sodium benzoate, sodium sorbate, potassium sorbate, citric acid One or more in potassium, sodium citrate, calcium citrate;
Described sugar molasse syrup is the one or many of sugar molasse syrup in National agricultural portion feedstuff catalogue Kind, for sucrose, glucose, fructose, glucose syrup, maltodextrin slurry/powder, high fructose syrup/powder, maltose One or more in slurry/powder, oligosaccharide syrup/powder, Sugar cane molasses, beet molasses;
Described liquid fat be in National agricultural portion feedstuff catalogue liquid fat one or more, for greatly Oleum Glycines and oil emulsion, Semen Maydis oil and oil emulsion thereof, Petiolus Trachycarpi oil and oil emulsion thereof, Testa oryzae oil and oil emulsion thereof, Oleum Cocois and oil emulsion, fish oil and animal oil and oil emulsion, Oleum Arachidis hypogaeae semen and oil emulsion thereof, Oleum Brassicae campestris and One or more in its oil emulsion, Oleum Camelliae and oil emulsion thereof and plants essential oil and extract thereof;
Described Chinese herbal medicine be in National agricultural portion Chinese herbal feed raw material and Chinese herbal veterinary medicine catalogue Chinese herbal medicine and One or more in extract, for the Radix Astragali and extract, the Cortex Eucommiae and extract thereof, Flos Lonicerae and carry Take one or more in thing, Fructus Crataegi and extract thereof, Pericarpium Citri Reticulatae and extract thereof, Flos Carthami and extract thereof;
Described vitamin is fatsoluble vitamin, vitamin B and vitamin C,.
A kind of molasses saccharide the most according to claim 3 acidifying and the preparation method of zymolysis feedstuff, it is special Levy and be,
Described molasses are one or both in Sugar cane molasses, beet molasses;
Described is one or both in Caulis Sacchari sinensis, Radix Betae containing sugar products;
Other organic acid fermentation liquid described is a kind of fermentation liquid or many in fumaric acid, acetic acid, propanoic acid, butanoic acid Plant fermentation liquid;
Described feedstuff is bean cake, Zein powder, DDGS, Testa oryzae, pasture powder, Chinese herbal medicine, or Above-mentioned feedstuff dilated product in one or more;
Described composite bacteria liquid be lactobacillus suspension, yeast liquid, more than two kinds of bacillus subtilis bacterium solution;
Described compound enzyme is commercial protein enzyme, xylanase, glucanase, mannonase pectase In more than two kinds;
Described commercially available feeding non-fermented source organic acid is one or more in formic acid, benzoic acid, fumaric acid;
Described commercially available feeding mineral acid is phosphoric acid;
Described acylate is one or both in calcium formate, sodium butyrate;
Described sugar molasse syrup be sucrose, glucose one or many in slurry, Sugar cane molasses, beet molasses Kind;
Described liquid fat is soybean oil and oil emulsion, Semen Maydis oil and oil emulsion thereof, Petiolus Trachycarpi oil and emulsifying thereof In oil, Testa oryzae oil and oil emulsion, Oleum Cocois and oil emulsion thereof, fish oil and animal oil and oil emulsion thereof one Plant or multiple;
Described Chinese herbal medicine is the Radix Astragali and extract thereof;
Described vitamin is vitamin C.
A kind of molasses saccharide the most according to claim 1 acidifying and the preparation method of zymolysis feedstuff, it is special Levying and be, the preparation condition of step 4. middle fermentation source organic acid concentrated solution is as follows:
(1) when preparing citric acid fermentation broth: product citric acid strain fermentation temperature: 5~50 DEG C;Fermentation pH: Natural;Fermented type: aerobic fermentation;Fermentation time: 24-96 hour;
(2) when, preparing lactic fermentation liquid: lactic acid producing strain fermentation temperature: 10~50 DEG C;Fermentation pH:4~ 10;Fermented type: anaerobic fermentation;Fermentation time: 24-144 hour;
(3), the weight ratio of citric acid fermentation broth, lactic fermentation liquid and other organic acid fermentation liquid be 100:0~ 500:0~300.
A kind of molasses saccharide the most according to claim 5 acidifying and the preparation method of zymolysis feedstuff, it is special Levying and be, the preparation condition of step 4. middle fermentation source organic acid concentrated solution is as follows:
(1) when, preparing citric acid fermentation broth: product citric acid waste residue strain fermentation temperature: 30~36 DEG C; Fermentation pH: natural;Fermented type: aerobic fermentation;Fermentation time: 48-60 hour;
(2) when, preparing lactic fermentation liquid: lactic acid producing strain fermentation temperature: 28~32 DEG C;Fermentation pH:6~ 7;Fermented type: anaerobic fermentation;Fermentation time: 36-48 hour;
(3), the weight ratio of citric acid fermentation broth, lactic fermentation liquid and other organic acid fermentation liquid be 100:30~ 60:20~30.
A kind of molasses saccharide the most according to claim 1 acidifying and the preparation method of zymolysis feedstuff, it is special Levying and be, the preparation condition of step 5. middle zymolysis slag dregs of rice culture medium wet basis is as follows:
(1), sugar slag wet basis, citric acid acid sludge wet basis, other organic acid acid sludge wet basis and feedstuff, dry Water purification, composite bacteria liquid, the weight ratio of compound enzyme are 100:0~300:0~300:0~500:0~1000: 0.1~100:0.00001~20;
(2), the lactobacillus suspension in composite bacteria liquid, yeast liquid, bacillus subtilis bacterium solution, lichens spore Bacillus liquid, bacillus bifidus liquid, aspergillus oryzae liquid, Brevibacterium acetylicum liquid, clostridium tyrobutyricum liquid, propionibacterium liquid Number of viable than for 100:0~300:0~300:0~100:0~100:0~100:0~50:0~ 50:0~50;
(3), the commercial protein enzyme in compound enzyme: xylanase: glucanase: mannase: pectin The weight ratio of enzyme is 100:0~500:0~500:0~500:0~500;
(4), enzymatic hydrolysis and fermentation temperature: 5~50 DEG C, enzymatic hydrolysis and fermentation initiates pH:3~8, and first aerobic enzymolysis is sent out Anaerase hydrolysis and fermentation 24-168 hour after ferment.
A kind of molasses saccharide the most according to claim 7 acidifying and the preparation method of zymolysis feedstuff, it is special Levying and be, the preparation condition of step 5. middle zymolysis slag dregs of rice culture medium wet basis is as follows:
(1), sugar slag wet basis, citric acid acid sludge wet basis, organic acid acid sludge wet basis and feedstuff, clean water, Composite bacteria liquid, the weight ratio of compound enzyme are 100:5~50:2~20:50~400:10~50:5~10: 0.003~3;
(2), the lactic acid bacteria culturers liquid in composite bacteria liquid: yeast seeds liquid: Bacillus licheniformis strain The number of viable of liquid is than for 100:2~20:1~10;
(3), the commercial protein enzyme in compound enzyme: xylanase: glucanase: mannase: pectin The weight ratio of enzyme is 100:30~50:30~50:30~50:10~30;
(4), enzymatic hydrolysis and fermentation temperature: 28~36 DEG C, enzymatic hydrolysis and fermentation initiates pH:6~8, first aerobic enzymolysis Anaerase hydrolysis and fermentation 60-72 hour after fermentation.
A kind of molasses saccharide the most according to claim 1 acidifying and the preparation method of zymolysis feedstuff, it is special Levying and be, the preparation condition of step 6. middle molasses saccharide acidifying and zymolysis feedstuff is as follows:
Fermentation source organic acid concentrated solution, zymolysis slag dregs of rice culture medium wet basis and commercially available feeding non-fermented source organic acid, Commercially available feeding mineral acid, acylate, sugar molasse syrup, liquid fat, Chinese herbal medicine and the weight of vitamin Amount than for 100:0~1000:0~200:0~200:0~200:0~300:0~300:0~50:0~ 50。
A kind of molasses saccharide the most according to claim 9 acidifying and the preparation method of zymolysis feedstuff, its Being characterised by, the preparation condition of step 6. middle molasses saccharide acidifying and zymolysis feedstuff is as follows:
Fermentation source organic acid concentrated solution, zymolysis slag dregs of rice culture medium wet basis and commercially available feeding non-fermented source organic acid, commercially available Feeding mineral acid, acylate, sugar molasse syrup, liquid fat, Chinese herbal medicine and the weight ratio of vitamin For 100:50~500:10~30:5~10:5~10:20~60:2~10:0~5:0~5.
CN201610285259.4A 2016-04-28 2016-04-28 Method for preparing molasses sugar acidification and glycolysis feed Pending CN105876160A (en)

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