CN105841995A - Plant rhizosphere and non-rhizosphere soil sampling device and sampling method - Google Patents
Plant rhizosphere and non-rhizosphere soil sampling device and sampling method Download PDFInfo
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- CN105841995A CN105841995A CN201610156564.3A CN201610156564A CN105841995A CN 105841995 A CN105841995 A CN 105841995A CN 201610156564 A CN201610156564 A CN 201610156564A CN 105841995 A CN105841995 A CN 105841995A
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- rhizosphere
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- cylindrical tube
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
Abstract
The invention discloses a plant rhizosphere and non-rhizosphere soil sampling device and sampling method. The device includes a culture box body and a plurality of collecting tubes; the culture box body comprises a cylindrical tube body and a base plate; the cylindrical box body is composed of two half tubes, two silicone rubber strips and a plurality of clamping hoops; two half tubes are in butt joint, and two butt joint parts of the two half tubes are sealed by the two silicone rubber strips respectively; after butt joint, the two half tubes are clamped tightly to form the cylindrical tube body through the clamping hoops; the lower end of the cylindrical tube body is fixed on the base plate; the collecting tubes are respectively and vertically inserted into a soil medium in the cylindrical tube body and are in honeycomb-shaped distribution. The method comprises the following five steps: 1) preparing culture soil; 2) filling the culture soil and burying the collecting tubes; 3) sowing plant seeds; 4) culturing plants; and 5) classifying and collecting soil samples. The sampling device has the advantages of simple structure, and can accurately distinguish areas surrounding root systems. The method is based on the device, is simple and easy to implement, and has accurate sampling and high sampling efficiency.
Description
Technical field
The present invention relates to plant rhizosphere soil sampling technique field, be specifically related to taking of a kind of plant rhizosphere and non-rhizosphere soils
Sampling device and sampling method.
Background technology
The concept of rhizosphere (Rhizosphere) was proposed by Lorenz Hiltner early than 1904, will number milli around root system
Rice, the soil body affected by root growth are defined as rhizosphere.The position that rhizosphere directly contacts with soil as root system of plant, its
Physics, chemistry and microbiological property, by root system of plant and the joint effect of soil, are also that soil microorganism is the most alive simultaneously
The place jumped.The device distinguishing rhizosphere and non-rhizosphere soils sample at present is locellus root case, the most square and cylindrical case
Body.It is partitioned into zones of different with the impenetrable nylon wire of root system, a side region of crop-planting is defined as rhizosphere, separately
Side is then territory, bulk soil.In order to improve the representativeness of sample, it is narrow that rhizosphere region is commonly designed all ratios, along with root
The growth of system, ultimately forms vertical, horizontal branch face or cylindrical root post, significantly limit root system in horizontal and vertical side
To free growth, with field in the case of natural the Root morphology of " Jie " font greatly differ from each other, and along with plant species
Planting the prolongation of time, the increase of root system volume, in root bag, the soil weight constantly improves, and significantly limit soil nutrient
To the migration of rhizosphere, easily cause the scarcity of plant growing later stage nutrient.Meanwhile, locellus root case warp during plant culturing
Go through repeatedly alternation of wetting and drying, at nylon wire, easily form the dry running through the soil body, have a strong impact on the seriality and of the soil body
Cause property, causes nutrient and moisture in the migration difficulty of the soil body.Additionally, the root method of trembling of classics is at soil water content and soil
Quality be suitable in the case of by artificially shaking off non-rhizosphere soils, gather a small amount of soil sticking on root system as Rhizosphere Soil,
But in the case of, soil sand grain content too low in soil moisture is higher, there is root system attachment soil amount very few, sampling efficiency is relatively
Low situation；And at the higher soil of moisture, as there is again the problem of sampling that Non-rhizosphere soil is difficult to shake off on rice soil.
Summary of the invention
For solving the problem that above-mentioned prior art exists, the invention provides the sampling of a kind of plant rhizosphere and non-rhizosphere soils
Device and sampling method, this sampler simple in construction, it is easy to make, with low cost, it is often more important that, this device energy
Enough in-situ acquisition rhizosphere and the soil of non-rhizosphere in the case of not affecting root system normal growth and not changing root system configuration
Sample, it is achieved root system peripheral region is accurately distinguished.
This sampling method relies on above-mentioned sampler, and sampling method is simple, and sampling is accurately and sampling efficiency is high.
Realizing the technical scheme that above-mentioned purpose of the present invention taked is:
A kind of plant rhizosphere and the sampler of non-rhizosphere soils, including cultivating casing and Duo Gen collection tube, cultivate casing bag
Including cylindrical tube and base plate, cylindrical box is made up of two semicanals, two silica gel strips and multiple clip, two semicanals pair
Connect, and two joints of two semicanals are sealed by two silica gel strips respectively, after two semicanal docking, constitute hollow by clip banding
Cylindrical tube, the equal opening in cylindrical tube two ends, the lower end of cylindrical tube is fixed on base plate, each collection tube
The equal opening in two ends, each collection tube inserts in the soil media in cylindrical tube with being respectively perpendicular, and collection tube is honeybee
Nest shape is distributed.
A height of 16 centimetres of described cylindrical tube, a diameter of 11 centimetres, a height of 10 millimeters of collection tube, diameter
It it is 6 millimeters.
Collection tube has 230-270 root.
Described semicanal and the material of base plate are polrvinyl chloride, and the material of collection tube is polypropylene, and the material of clip is not
Rust steel.
A kind of based on above-mentioned plant rhizosphere with the sampling method of the sampler of non-rhizosphere soils, comprise the steps:
1) taking wind desiceted soil, the type of wind desiceted soil can be adjusted according to floristic difference, is smashed by wind desiceted soil, via
Footpath is the sieve of 2mm, then admixes fertilizer, and fertilizer herein is adjusted according to the kind of the plant cultivated, obtains
Culture soil；
2) taking the culture soil of half, uniformly water spray makes its mass water content is 15 ± 5%, is added into after water spray
In cylindrical tube, pave, be compacted, then all of collection tube is inserted in culture soil with being respectively perpendicular, make each
The middle and lower part of collection tube is respectively positioned in culture soil, ensures that collection tube is cellular distribution simultaneously, then by remaining compost
Earth loads in cylindrical tube, it is ensured that all of collection tube is cultured soil and fills up；
3) choose full, healthy plant seed, then plant seed kind entered in the culture soil in cylindrical tube body,
Watering after earthing, the mass water content making the culture soil of cylindrical tube is 25 ± 5%, herein it should be noted that one
As when cultivating dry farming plant, the mass water content watered to culture soil is 25 ± 5%, when the plant cultivated is paddy field or wet
During ground plant, water and can reach waterflooding state to culture soil；
4) whole sampler is placed in greenhouse or illumination cultivation room cultivation；
5) according to test requirements document, collecting rhizosphere and non-rhizosphere soils sample period in plant particular growth, plantation same is made
Thing can sample at different time/growth period, and growing different crops also can sample in the same time, so collecting sample
Time is carried out according to test requirements document, and the collection method of rhizosphere and non-rhizosphere soils sample is: after A, removal clip, use cutter
Sheet cuts cylindrical tube open along silica gel strip, removes above each collection tube the most respectively, the soil of lower section adhesion and root system, then
Grip each collection tube respectively with tweezers, the soil of attachment cleaned on each collection tube outer wall by cotton swab, if having root system into or through
This collection tube, then judge soil in this collection tube as rhizosphere soil, if not having root system to enter this collection tube, then this collection
Soil in pipe is non-rhizosphere soils；Collection tube is classified by B, the kind of foundation soil, is i.e. divided into equipped with rhizosphere soil
Rhizosphere soil collection tube and the non-rhizosphere soils collection tube equipped with non-rhizosphere soils, by the rhizosphere in each rhizosphere soil collection tube
Mixing with soil is uniform, obtains rhizosphere soil sample, by the non-rhizosphere soils mix homogeneously of each non-rhizosphere soils collection tube, obtains non-
Rhizosphere soil sample, saves backup after the rhizosphere soil sample of gained and non-rhizosphere soils sample liquid nitrogen flash freezer-80 DEG C.
Compared with prior art, advantages of the present invention and having the beneficial effects that:
1) this device provides growth naturally and the expansion of enough growing spaces, beneficially root system to root system, it is to avoid point
The change of the Root morphology that room root case is caused so that the growth of root system more fit field condition root system naturally growth shape
Condition.
2) this device takes full advantage of the geotropism of root growth, is nested in outside root system by some collection tubes vertically buried underground,
Effectively prevent because raising or the root biomass of excessive the caused root region soil unit weight of root biomass cross rootlet district soil
Earth can not effectively embody the problems such as rhizosphere effect, substantially increases representativeness and the effectiveness of sample.
3) this device is easy and simple to handle, with low cost, technology requirement is relatively low, is suitable for various plants, nonirrigated farmland, paddy field life
State system rhizosphere and non-rhizospheric microbe characteristic, the research of soil nutrient.
4) the method can be with in situ quantitation noiseless herborization rhizosphere and non-rhizosphere soils, by extracting soil microorganism
DNA, utilizes the means such as real-time fluorescence quantitative PCR, T-RFLP, high-flux sequence to characterize in molecular biology level
Rhizosphere and non-rhizospheric microbe Monopterus albus and multifarious change, it is also possible to by analyzing the conversion of soil nutrient, root system
The component of deposit and content, and then provide fundamental basis for inquiring into rhizospheric environment energy cycle of matter.
Accompanying drawing explanation
Fig. 1 is the structural representation of the sampler of the plant rhizosphere of the present invention and non-rhizosphere soils.
Fig. 2 is the top view of Fig. 1.
Wherein, 1-cylindrical tube, 2-clip, 3-collection tube, 4-silica gel strip, 5-base plate.
Detailed description of the invention
Below in conjunction with the accompanying drawings the plant rhizosphere of the present invention and the sampler of non-rhizosphere soils are described in detail.
The present invention provide plant rhizosphere and non-rhizosphere soils sampler structure as depicted in figs. 1 and 2, including training
Support casing and Duo Gen collection tube 3, cultivate casing and include cylindrical tube 1 and base plate 5.
Cylindrical tube is made up of two semicanals, two silica gel strips 4 and multiple clip 2, two semicanal docking, and two semicanals
Two joints sealed by two silica gel strips 4 respectively, constituted the cylindrical tube of hollow after two semicanals docking by clip banding
Body 1, the lower end of cylindrical tube 1 is fixed on base plate 5, upper end open.In the present embodiment, the height of cylindrical tube
For 16cm, a diameter of 11cm.
The equal opening in two ends of each collection tube, each collection tube inserts in the soil media in cylindrical tube body with being respectively perpendicular,
And collection tube is cellular distribution.In the present embodiment, collection tube has 230-270 root, a height of 10mm of collection tube, directly
Footpath is 6mm.
Described semicanal and the material of base plate are polrvinyl chloride, and the material of collection tube is polypropylene, and the material of clip is not
Rust steel.
The feature of this device is: each collection tube forms an independent soil body in cylindrical tube, is nested in root
Fasten or independent of root system outside, the soil being enclosed within the collection tube on root system because being affected by root system, its physics, change
Learn and Microbiological Characteristics is different from the soil in the collection tube outside root system, form an effective Rhizosphere Soil collecting unit.
Meanwhile, for ensureing the normal growth of root system, collection tube Design of length is 10mm, although this is due to longer collection tube
More pedotheque can be obtained, but hinder the migration of moisture and nutrient, affect root growth；And too short collection
Pipe, can cause pedotheque amount very few, representative strong, need to increase when device makes and samples collection tube quantity,
Thus improve device making, the time cost of sample collecting and human cost.
Below in conjunction with specific embodiment, the sampling method of sampler based on above-mentioned plant rhizosphere and non-rhizosphere soils is carried out
Illustrate.
Embodiment 1
The present embodiment illustrates as a example by moisture soil-Maize Combination.
1) making of casing is cultivated: docked by two PVC semicanals, for ensureing the sealing of cylindrical tube, two
Two joints of PVC semicanal filling gel bar respectively, by two stainless steel lathe dog after two PVC semicanal docking good seal
Banding, the top of one of them stainless steel lathe dog banding two PVC semicanal, another stainless steel lathe dog banding two PVC half
The cylindrical tube of the bottom of pipe, hollow and both ends open makes complete, then at the bonding PVC in one end of cylindrical tube
Base plate, incubator system is made complete, cultivates box bottom and put on the ground during use, cylindrical tube opening up；
2) taking the moisture soil that 1kg air-dries, smash, crossing aperture is the sieve of 2mm, removes visible stone and plant residue,
Then admix the fertilizer needed for Semen Maydis is cultivated, such as carbamide, potassium dihydrogen phosphate, potassium chloride and trace-element fertilizer, trained
Support soil；
3) taking the culture soil of half, uniformly water spray makes its mass water content is 15%, is added into circle after water spray
In cylinder, pave, be compacted, then all of collection tube (230-270 root) is inserted in culture soil respectively,
The middle and lower part making each collection tube is respectively positioned in culture soil, ensures that collection tube is cellular distribution simultaneously, then by remaining
Culture soil loads in cylindrical tube, it is ensured that all of collection tube is cultured soil and fills up；
4) choose 2 corn seeds full, that show money or valuables one carries unintentionally, then 2 corn seed kinds are entered the cultivation in cylindrical tube body
In soil, watering after earthing, the mass water content making the culture soil of cylindrical tube is 25%；
5) whole sampler is placed in illumination is good, the greenhouse of proper temperature or illumination cultivation room are cultivated；
6) collecting rhizosphere and non-rhizosphere soils sample after emerging 30 days, the collection method of rhizosphere and non-rhizosphere soils sample is:
A, remove after clip, cut cylindrical tube with blade open along silica gel strip, the most careful remove above each collection tube, lower section is glued
Soil even and root system, then grip each collection tube respectively with tweezers, and the soil of attachment on each collection tube outer wall cleaned by cotton swab
To facilitate the soil type judged in this collection tube, if having root system into or through this collection tube, then judge in this collection tube
Soil be rhizosphere soil, if not having root system to enter this collection tube, then the soil in this collection tube is non-rhizosphere soils；B、
According to the kind of soil, collection tube is classified, be i.e. divided into the rhizosphere soil collection tube equipped with rhizosphere soil and equipped with Non-rhizosphere soil
The non-rhizosphere soils collection tube of earth, by the rhizosphere soil mix homogeneously in each rhizosphere soil collection tube, obtains rhizosphere soil sample,
By the non-rhizosphere soils mix homogeneously of each non-rhizosphere soils collection tube, obtain non-rhizosphere soils sample, by the rhizosphere soil of gained
After sample and non-rhizosphere soils sample liquid nitrogen flash freezer ,-80 DEG C save backup.
Repeating above-mentioned sampling three times, collection tube is carried out classified statistic by every sub-sampling, and statistical result is as shown in table 1:
Rhizosphere and non-rhizosphere soils collection tube number in table 1 incubator
As shown in Table 1, the ratio of rhizosphere soil collection tube and non-rhizosphere soils collection tube in casing of cultivating is 5:1-8:1.
The rhizosphere soil sample obtained by every sub-sampling and non-rhizosphere soils sample carry out microbial DNA and extract test, examination
Test result as shown in table 2:
Table 2 rhizosphere and non-rhizosphere soils microbial DNA concentration
As shown in Table 2, in rhizosphere soil the DNA concentration of microorganism apparently higher than the DNA of microorganism in non-rhizosphere soils
Concentration, both differences are 12.6-16.8ng/ μ L, and on coincidence theory, rhizosphere soil microorganism amount is micro-higher than non-rhizosphere soils
The deduction of Biomass.
Claims (5)
1. a plant rhizosphere and the sampler of non-rhizosphere soils, it is characterised in that: include cultivating casing and Duo Gen gathers
Pipe, cultivates casing and includes cylindrical tube and base plate, and cylindrical box is by two semicanals, two silica gel strips and multiple clip
Constitute, two semicanal docking, and two joints of two semicanals are sealed by two silica gel strips respectively, by card after two semicanal docking
Hoop banding constitutes the cylindrical tube of hollow, and the equal opening in cylindrical tube two ends, the lower end of cylindrical tube is fixed on base plate
On, the equal opening in two ends of each collection tube, each collection tube inserts in the soil media in cylindrical tube with being respectively perpendicular,
And collection tube is cellular distribution.
Plant rhizosphere the most according to claim 1 and the sampler of non-rhizosphere soils, it is characterised in that: described
A height of 16 centimetres of cylindrical tube, a diameter of 11 centimetres, a height of 10 millimeters of collection tube, a diameter of 6 millimeters.
Plant rhizosphere the most according to claim 2 and the sampler of non-rhizosphere soils, it is characterised in that: collection tube
There is 230-270 root.
Plant rhizosphere the most according to claim 1 and the sampler of non-rhizosphere soils, it is characterised in that: described
The material of semicanal and base plate is polrvinyl chloride, and the material of collection tube is polypropylene, and the material of clip is rustless steel.
5., based on the plant rhizosphere described in claim 1 and a sampling method for the sampler of non-rhizosphere soils, it is special
Levy and be to comprise the steps:
1) taking wind desiceted soil, smashed by wind desiceted soil, crossing aperture is the sieve of 2mm, then admixes fertilizer, fertilizer root herein
It is adjusted according to the kind of the plant cultivated, obtains culture soil；
2) taking the culture soil of half, uniformly water spray makes its mass water content is 15 ± 5%, is added into after water spray
In cylindrical tube, pave, be compacted, then all of collection tube is inserted in culture soil with being respectively perpendicular, make each
The middle and lower part of collection tube is respectively positioned in culture soil, ensures that collection tube is cellular distribution simultaneously, then by remaining compost
Earth loads in cylindrical tube, it is ensured that all of collection tube is cultured soil and fills up；
3) choose full, healthy plant seed, then plant seed kind entered in the culture soil in cylindrical tube body,
Watering after earthing, the mass water content making the culture soil of cylindrical tube is 25 ± 5%；
4) whole sampler is placed in greenhouse or illumination cultivation room cultivation；
5) according to test requirements document, rhizosphere and non-rhizosphere soils sample, rhizosphere and non-rhizosphere are collected period in plant particular growth
The collection method of pedotheque is: after A, removal clip, cuts cylindrical tube with blade open along silica gel strip, goes the most respectively
Except above each collection tube, the soil of lower section adhesion and root system, then grip each collection tube respectively with tweezers, cotton swab is cleaned respectively
The soil of attachment on collection tube outer wall, if having root system into or through this collection tube, then judge soil in this collection tube as
Rhizosphere soil, if not having root system to enter this collection tube, then the soil in this collection tube is non-rhizosphere soils；B, foundation soil
Collection tube is classified by the kind of earth, is i.e. divided into the rhizosphere soil collection tube equipped with rhizosphere soil and non-equipped with non-rhizosphere soils
Rhizosphere soil collection tube, by the rhizosphere soil mix homogeneously in each rhizosphere soil collection tube, obtains rhizosphere soil sample, will be each
The non-rhizosphere soils mix homogeneously of non-rhizosphere soils collection tube, obtains non-rhizosphere soils sample, by the rhizosphere soil sample of gained
Save backup with after non-rhizosphere soils sample liquid nitrogen flash freezer-80 DEG C.
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| CN201610156564.3A CN105841995B (en) | 2016-03-18 | 2016-03-18 | The sampler and sampling method of a kind of plant rhizosphere and non-rhizosphere soils |
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| CN201610156564.3A CN105841995B (en) | 2016-03-18 | 2016-03-18 | The sampler and sampling method of a kind of plant rhizosphere and non-rhizosphere soils |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106226172A (en) * | 2016-08-31 | 2016-12-14 | 云南省交通规划设计研究院 | A kind of radical operators Situ Computation device and application process |
| CN106769166A (en) * | 2016-12-06 | 2017-05-31 | 河海大学 | A kind of experimental provision for the fine Stratified Sampling of green plant rhizosphere soil |
| CN113702096A (en) * | 2021-09-18 | 2021-11-26 | 河南工程学院 | Plant rhizosphere box, rhizosphere box soil sample collecting device and collecting method |
| CN115005028A (en) * | 2022-06-24 | 2022-09-06 | 临沂市农业科学院 | Device and method for collecting pepper rhizosphere secretion |
Family Cites Families (5)
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| CN101082547B (en) * | 2007-07-12 | 2010-09-08 | 浙江大学 | Plants root box culturing device used for rhizosphere soil sampling |
| CN102160504A (en) * | 2011-01-13 | 2011-08-24 | 南京林业大学 | Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage |
| CN202485959U (en) * | 2012-03-05 | 2012-10-10 | 华中农业大学 | Root box testing device for plant cultivation |
| CN103004516B (en) * | 2013-01-09 | 2015-05-06 | 云南农业大学 | Container for analyzing crop root interaction in different cropping patterns |
| CN204244801U (en) * | 2014-11-13 | 2015-04-08 | 广东省生态环境与土壤研究所 | A kind of root bag field planting easy device studying rhizosphere soil character |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226172A (en) * | 2016-08-31 | 2016-12-14 | 云南省交通规划设计研究院 | A kind of radical operators Situ Computation device and application process |
| CN106769166A (en) * | 2016-12-06 | 2017-05-31 | 河海大学 | A kind of experimental provision for the fine Stratified Sampling of green plant rhizosphere soil |
| CN113702096A (en) * | 2021-09-18 | 2021-11-26 | 河南工程学院 | Plant rhizosphere box, rhizosphere box soil sample collecting device and collecting method |
| CN113702096B (en) * | 2021-09-18 | 2023-12-08 | 河南工程学院 | Rhizosphere box soil sample collection device and collection method |
| CN115005028A (en) * | 2022-06-24 | 2022-09-06 | 临沂市农业科学院 | Device and method for collecting pepper rhizosphere secretion |
| CN115005028B (en) * | 2022-06-24 | 2023-12-15 | 临沂市农业科学院 | Pepper rhizosphere secretion collecting device and method |
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Address after: 415700 yuzhangbei Road, Zhangjiang Town, Taoyuan County, Changde, Hunan 14 Applicant after: Institute of Subtropical Agriculture, Chinese Academy of Sciences Address before: 410125 644 Yuanda two road, Furong district, Changsha, Hunan Applicant before: Institute of Subtropical Agriculture, Chinese Academy of Sciences |
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