CN103039243B - Method for determining companion planting of agricultural crops - Google Patents
Method for determining companion planting of agricultural crops Download PDFInfo
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- CN103039243B CN103039243B CN201210595996.6A CN201210595996A CN103039243B CN 103039243 B CN103039243 B CN 103039243B CN 201210595996 A CN201210595996 A CN 201210595996A CN 103039243 B CN103039243 B CN 103039243B
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Abstract
The invention discloses a method for determining companion planting of agricultural crops. The method includes: selecting suitable flowerpots and nylon bags according to the size and the quantity of cultivated crops, and placing two equal-sized nylon bags into the middle of one flowerpot; uniformly mixing soil with organic fertilizers according to the proportion of 10:1 so that a culture medium is prepared, and filling the sterilized culture medium into the nylon bags and the flowerpot; uniformly sowing three first crops in the nylon bags, sowing three second crops between a nylon membrane and the flowerpot and corresponding to the first crops in position, and marking positions by a permanent marker as the shortest distance between each first crop and the nylon membrane is as same as the shortest distance between each second crop and the nylon membrane; respectively sampling and determining rhizosphere soil of the first intercrops, the second intercrops, monoculture first crops and monoculture second crops and soil on the nylon membrane in the suitable growing periods of the first crops and the second crops; and analyzing soil microorganism crops according to soil sampling and determining results so as to determine whether the first crops and the second crops are suitable for companion planting or not. Therefore, the method is timesaving and laborsaving.
Description
Technical field
The invention belongs to agricultural crops collocation planting technology field, relate in particular to a kind of method of definite agricultural crops collocation plantation.
Background technology
The continuous growth of population, food problem becomes one of significant problem that the world faces.Therefore utilize the diversity growing of crop to increase grain yield and also become emphasis and the bright spot of current research thereupon.Existing many research reports, diversity growing can improve field microclimate, reduce generation and the development of damage by disease and insect, effectively improves the grain yield of unit are.For the cropping pattern obtaining, not only need to analyze hereditary information and the biological character of crop, also need to carry out more than 2 years or 2 years field trial, detect the quality of cereal crops and the output of unit are grain.And can not ensure the crop collocation mode obtaining in the short period of time.
Due at occurring in nature, each plant corpus is not an independent isolated individuality, and itself and the microorganism of rhizosphere and other plant exist certain interaction.The plant corpus of living is by active secretion, seepage, wearing and tearing, wound and dead cell, from root system, releasing organics and inorganic matter are in rhizosphere, thereby for edaphon provides sufficient nutriment, and different rhizospheric microorganisms has certain selectivity to the kind of plant, it affects quantity and the population structure of rhizospheric microorganism.Otherwise microorganism also has facilitation to the growth of root system of plant, it can affect the formation of physiological function and the root secretion of root consumingly.Even the output of the growing of the quality and quantity of rhizosphere microorganism and plant, crop forms good correlation.And microbiologic population is from non-rhizosphere to interior rhizosphere, has the advantages that continuity distributes.Therefore, can be used in by population and the microbial biomass of analysis and monitoring crop edaphon the effect of evaluating diversity growing pattern.
Adopt at present intercropping mode screening to determine whether crop looks good with the method for plantation, time-consuming, effort, practicality be poor, it is not high to produce effects.
Summary of the invention
The invention provides a kind of method of definite agricultural crops collocation plantation, be intended to solve and adopt intercropping mode screening to determine whether crop looks good with the method for plantation, time-consuming, effort, practicality are poor, the not high problem of producing effects.
A kind of method that the object of the present invention is to provide definite agricultural crops collocation plantation, the method comprises the following steps:
Step 1, according to the number of the size of institute raise crop and raise crop, selects suitable flowerpot and Nylon Bag, and the Nylon Bag of two formed objects is put into the center position of flowerpot;
Step 2, mixes soil and fertilizer in the ratio of 10: 1, make culture matrix, and culture matrix is put in to sterilizing 40min at 121 DEG C, and the culture matrix after sterilizing is filled in Nylon Bag and flowerpot;
Step 3 is broadcast equably 3 crops one in Nylon Bag, and between nylon membrane and flowerpot, 1 crop two is respectively broadcast in the position corresponding with crop one, broadcasts altogether 3, and crop one is identical apart from the beeline of nylon membrane with crop two, and uses permanent pen mark position;
Step 4, after planting covers with ranker, and waters, and growing period management is with conventional;
Step 5, measured the soil sample on rhizosphere soil and the nylon membrane of interculture one, interculture two, nonoculture crop one and nonoculture crop two respectively in crop one and the suitable growth phase of crop two;
Step 6, analyzes edaphon crop by soil sample measurement result, determines whether crop one and crop two look good with plantation.
Further, in step 1, according to the shape of flowerpot and internal diameter, be that the nylon membrane of 30 microns is curling by aperture, diameter equals respectively 1/2 of the upper and lower interior diameter of flowerpot, by interface nylon line suture, and nylon membrane is done at the bottom of one, bottom is corresponding with flowerpot bottom, and flowerpot is large mouthful of handle, Nylon Bag is also made large mouthful of handle, the Nylon Bag of two formed objects is put into the center position of flowerpot.
Further, in step 2, the culture matrix after sterilizing is first filled in the tubbiness Nylon Bag in the inside, recharges flowerpot, make Nylon Bag be positioned at the center of flowerpot, and inside and outside soil height is consistent, and lower than the height of Nylon Bag.
Further, when selected crop one is wheat, when crop two is broad bean, in Nylon Bag, broadcast equably 3 wheats, between nylon membrane and basin, 1 broad bean is respectively broadcast in the position corresponding with wheat, broadcasts altogether 3, wheat is identical apart from the beeline of nylon membrane with broad bean, and uses permanent pen mark position;
After planting cover with ranker, and water, growing period management is with conventional;
Respectively the soil sample on Fababean Intercropping, intercropping wheat, nonoculture broad bean and nonoculture wheat rhizosphere soil and nylon membrane is measured in the pod bearing period of broad bean and the shooting stage of wheat.
Further, in step 5, the implementation method that the soil sample on crop rhizosphere soils and nylon membrane is measured is:
One of the inside Nylon Bag is taken out carefully from flowerpot, take out in the markd position of Nylon Bag, the degree of depth that fetches earth is 10-15cm;
Vertically cut off along mark with scissors carefully at outside Nylon Bag, get the soil sample in Nylon Bag outside at its 10-15cm place;
Respectively soil sample is taken back to laboratory with the paper bag of sterilizing, at room-dry, measure respectively the biomass of Soil Microorganism by flat band method, every basin 12 parts of soil samplings altogether, Fababean Intercropping, intercropping wheat, only make broad bean and only make each 3 parts of wheat.
Further, the performing step of the flat band method of the biomass of described mensuration Soil Microorganism is:
Indoor every part of air-dry soil sample is smash to powdered, take 1 gram of dry ground and put into the triangular flask of 20 milliliters that 9 milliliters of sterile purified waters are housed, hand 10 minutes, microbial cell is fully disperseed, obtain 10-1 soil dilution;
Draw 1 milliliter of 10-1 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-2 soil dilution;
Draw 1 milliliter of 10-2 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-3 soil dilution;
Draw 1 milliliter of 10-3 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-4 soil dilution;
Draw 1 milliliter of 10-4 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-5 soil dilution;
Draw 1 milliliter of 10-5 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-6 soil dilution;
Detect actinomycetic quantity with 10-3,10-4,10-5,10-6 dilution 1 milliliter of dull and stereotyped bacterial detection amount of painting beef extract-peptone agar medium, potato agar culture medium flat plate detection fungi amount and starch ammonium salt culture medium flat plate respectively, the flat-plate inverted coating is placed in the incubator of 28 DEG C and cultivates, within 5-10 days, record afterwards the clump count on flat board, and calculate contained bacterium amount in every gram of soil by optimal extension rate.
The method of definite agricultural crops collocation plantation provided by the invention, according to the size of institute raise crop and raise crop number, select suitable flowerpot and Nylon Bag, and the Nylon Bag of two formed objects put into the center position of flowerpot; Soil and fertilizer are mixed in the ratio of 10: 1, make culture matrix, culture matrix is put in to sterilizing 40min at 121 DEG C, the culture matrix after sterilizing is filled in Nylon Bag and flowerpot; In Nylon Bag, broadcast equably 3 crops one, between nylon membrane and flowerpot, 1 crop two is respectively broadcast in the position corresponding with crop one, broadcasts altogether 3, and crop one is identical apart from the beeline of nylon membrane with crop two, and uses permanent pen mark position; After planting cover with ranker, and water, growing period management is with conventional; Respectively the soil sample on rhizosphere soil and the nylon membrane of interculture one, interculture two, nonoculture crop one and nonoculture crop two is measured in crop one and the suitable growth phase of crop two; Analyze edaphon crop by soil sample measurement result, determine whether crop one and crop two look good with plantation, time saving and energy saving, practical, the height that produces effects, has stronger propagation and employment and is worth.
Brief description of the drawings
Fig. 1 is the realization flow figure of the method for the definite agricultural crops collocation plantation that provides of embodiment provided by the invention;
Fig. 2 is arrange in pairs or groups in the flowerpot schematic diagram of plantation of agricultural crops that the embodiment of the present invention provides.
In figure: 1, crop one; 2, crop two; 3, Nylon Bag; 4, flowerpot; 5, mark position.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is described in further detail.Should be appreciated that specific embodiment described herein is only in order to explain the present invention, and be not used in restriction invention.
The flow process of the method that definite agricultural crops collocation that Fig. 1 shows the embodiment of the present invention to be provided is planted.
A kind of method that the object of the present invention is to provide definite agricultural crops collocation plantation, the method comprises the following steps:
Step S101, according to the number of the size of institute raise crop and raise crop, selects suitable flowerpot 4 and Nylon Bag 3, and the Nylon Bag of two formed objects 3 is put into the center position of flowerpot 4;
Step S102, mixes soil and fertilizer in the ratio of 10: 1, make culture matrix, and culture matrix is put in to sterilizing 40min at 121 DEG C, and the culture matrix after sterilizing is filled in Nylon Bag 3 and flowerpot 4;
Step S103, in Nylon Bag 3, broadcast equably 3 crops 1, between nylon membrane and flowerpot 4,1 crop 22 is respectively broadcast in the position corresponding with crop 1, broadcasts altogether 3, crop 1 is identical apart from the beeline of nylon membrane with crop 22, and draws mark position 5 with permanent pen;
Step S104, after planting covers with ranker, and waters, and growing period management is with conventional;
Step S105, measured the soil sample on rhizosphere soil and the nylon membrane of interculture 1, interculture 22, nonoculture crop 1 and nonoculture crop 22 respectively in crop 1 and the suitable growth phase of crop 22;
Step S106, analyzes edaphon crop by soil sample measurement result, determines whether crop 1 and crop 22 look good with plantation.
In embodiments of the present invention, in step S101, according to the shape of flowerpot 4 and internal diameter, be that the nylon membrane of 30 microns is curling by aperture, diameter equals respectively 1/2 of flowerpot 4 upper and lower interior diameters, by interface nylon line suture, and nylon membrane is done at the bottom of one, bottom is corresponding with flowerpot 4 bottoms, and flowerpot 4 is large mouthful of handles, Nylon Bag 3 is also made large mouthful of handle, the Nylon Bag of two formed objects 3 is put into the center position of flowerpot 4.
In embodiments of the present invention, in step S102, the culture matrix after sterilizing is first filled in the tubbiness Nylon Bag 3 in the inside, recharges flowerpot 4, make Nylon Bag 3 be positioned at the center of flowerpot 4, and inside and outside soil height is consistent, and lower than the height of Nylon Bag 3.
In embodiments of the present invention, when selected crop 1 is wheat, when crop 22 is broad bean, in Nylon Bag 3, broadcast equably 3 wheats, between nylon membrane and basin, 1 broad bean is respectively broadcast in the position corresponding with wheat, broadcast altogether 3, wheat is identical apart from the beeline of nylon membrane with broad bean, and draws mark position 5 with permanent pen;
After planting cover with ranker, and water, growing period management is with conventional;
Respectively the soil sample on Fababean Intercropping, intercropping wheat, nonoculture broad bean and nonoculture wheat rhizosphere soil and nylon membrane is measured in the pod bearing period of broad bean and the shooting stage of wheat.
In embodiments of the present invention, in step S105, the implementation method that the soil sample on crop rhizosphere soils and nylon membrane is measured is:
One of the inside Nylon Bag 3 is taken out carefully from flowerpot 4, take out in the markd position of Nylon Bag 3, the degree of depth that fetches earth is 10-15cm;
Vertically cut off along mark with scissors carefully at outside Nylon Bag 3, get the soil sample in Nylon Bag 3 outsides at its 10-15cm place;
Respectively soil sample is taken back to laboratory with the paper bag of sterilizing, at room-dry, measure respectively the biomass of Soil Microorganism by flat band method, every basin 12 parts of soil samplings altogether, Fababean Intercropping, intercropping wheat, only make broad bean and only make each 3 parts of wheat.
In embodiments of the present invention, the performing step of the flat band method of the biomass of mensuration Soil Microorganism is:
Indoor every part of air-dry soil sample is smash to powdered, take 1 gram of dry ground and put into the triangular flask of 20 milliliters that 9 milliliters of sterile purified waters are housed, hand 10 minutes, microbial cell is fully disperseed, obtain 10-1 soil dilution;
Draw 1 milliliter of 10-1 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-2 soil dilution;
Draw 1 milliliter of 10-2 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-3 soil dilution;
Draw 1 milliliter of 10-3 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-4 soil dilution;
Draw 1 milliliter of 10-4 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-5 soil dilution;
Draw 1 milliliter of 10-5 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-6 soil dilution;
Detect actinomycetic quantity with 10-3,10-4,10-5,10-6 dilution 1 milliliter of dull and stereotyped bacterial detection amount of painting beef extract-peptone agar medium, potato agar culture medium flat plate detection fungi amount and starch ammonium salt culture medium flat plate respectively, the flat-plate inverted coating is placed in the incubator of 28 DEG C and cultivates, within 5-10 days, record afterwards the clump count on flat board, and calculate contained bacterium amount in every gram of soil by optimal extension rate.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
The embodiment of the present invention provides a kind of method that whether looks good with plantation by Soil Microbes crop, steps of the method are the number according to the size of institute raise crop and raise crop, select suitable flowerpot 4, with according to the shape of flowerpot 4 and internal diameter, the nylon membrane that aperture is about to 30 microns is curling, its diameter equals respectively on flowerpot 4, 1/2 of lower interior diameter, by interface nylon line suture, and nylon membrane is done at the bottom of one, bottom is corresponding with flowerpot 4 bottoms, flowerpot 4 is large mouthful of handles, Nylon Bag 3 is also made large mouthful of handle, the Nylon Bag of two formed objects 3 is put into flowerpot 4 to be hit exactly,
Soil and fertilizer are mixed in the ratio of 10: 1, make culture matrix, culture matrix is put in to sterilizing 40min at 121 DEG C, culture matrix after sterilizing is first filled in the tubbiness Nylon Bag 3 in the inside, recharge flowerpot 4, so that Nylon Bag 3 is positioned at the center of flowerpot 4, and inside and outside soil height is consistent, and lower than Nylon Bag 3; In Nylon Bag 3, broadcast equably 3 wheats or broad bean, between nylon membrane and basin, 1 broad bean or wheat are respectively broadcast in the position corresponding with wheat, broadcast altogether 3, wheat is identical apart from the beeline of nylon membrane with broad bean, and draws mark position 5 (Fig. 2) with permanent pen; After planting cover with ranker, and water; Growing period management is with conventional; Respectively the soil sample on Fababean Intercropping, intercropping wheat, nonoculture broad bean and nonoculture wheat rhizosphere soil and nylon membrane is measured in the pod bearing period of broad bean and the shooting stage of wheat.
In said method, soil sample method for measuring is that one of the inside Nylon Bag 3 is taken out carefully from flowerpot 4, takes out in the markd position of Nylon Bag 3, and the degree of depth that fetches earth is 10-15cm; Vertically cut off along mark with scissors carefully at outside Nylon Bag 3 in addition, get the soil sample in Nylon Bag 3 outsides at its 10-15cm place; Respectively soil sample is taken back to laboratory with the paper bag of sterilizing, at room-dry, measure respectively the biomass of Soil Microorganism by flat band method, every basin 12 parts of soil samplings altogether, Fababean Intercropping, intercropping wheat, only make broad bean and only make each 3 parts of wheat.
The flat band method of measuring the biomass of Soil Microorganism is: indoor every part of air-dry soil sample is smash powdered by (1), take 1 gram of dry ground and put into the triangular flask of 20 milliliters that 9 milliliters of sterile purified waters are housed, hand 10 minutes, microbial cell is fully disperseed, obtain 10-1 soil dilution; (2) draw 1 milliliter of 10-1 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-2 soil dilution; (3) draw 1 milliliter of 10-2 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-3 soil dilution; (4) draw 1 milliliter of 10-3 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-4 soil dilution; The like obtain the soil such as 10-5,10-6 dilution.Be coated with the dull and stereotyped bacterial detection amount of beef extract-peptone agar medium, potato agar culture medium flat plate detection fungi amount and starch ammonium salt culture medium flat plate with 1 milliliter of the dilution such as 10-3,10-4,10-5,10-6 respectively and detect actinomycetic quantity, the flat-plate inverted coating is placed in the incubator of 28 DEG C and cultivates, within 5-10 days, record afterwards the clump count on flat board, and calculate contained bacterium amount (table 1) in every gram of soil by optimal extension rate.
The comparison of the clean work of table 1 and intercropping soil microbe quantity
Under intercropping, plant as can be seen from Table 1 the bacterium, fungi and the actinomycetic quantity that in the soil of wheat and broad bean, detect all more than only making broad bean and only making bacterium, fungi and the actinomycetic quantity in wheat soil.Show that according to the result of field experiment for many years broad bean and wheat intercropping really can effectively improve the output of cereal crops.Therefore the method that whether looks good with plantation by Soil Microbes crop is feasible.
The method of the definite agricultural crops collocation plantation that the embodiment of the present invention provides, according to the size of institute raise crop and raise crop number, select suitable flowerpot 4 and Nylon Bag 3, and the Nylon Bag of two formed objects 3 put into the center position of flowerpot 4; Soil and fertilizer are mixed in the ratio of 10: 1, make culture matrix, culture matrix is put in to sterilizing 40min at 121 DEG C, the culture matrix after sterilizing is filled in Nylon Bag 3 and flowerpot 4; In Nylon Bag 3, broadcast equably 3 crops 1, between nylon membrane and flowerpot 4,1 crop 22 is respectively broadcast in the position corresponding with crop 1, broadcast altogether 3, crop 1 is identical apart from the beeline of nylon membrane with crop 22, and draws mark position 5 with permanent pen; After planting cover with ranker, and water, growing period management is with conventional; Respectively the soil sample on rhizosphere soil and the nylon membrane of interculture 1, interculture 22, nonoculture crop 1 and nonoculture crop 22 is measured in crop 1 and the suitable growth phase of crop 22; Analyze edaphon crop by soil sample measurement result, determine whether crop 1 and crop 22 look good with plantation, time saving and energy saving, practical, the height that produces effects, has stronger propagation and employment and is worth.
These are only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (5)
1. a method for definite agricultural crops collocation plantation, is characterized in that, the method comprises the following steps:
Step 1, according to the number of the size of institute raise crop and raise crop, selects suitable flowerpot and Nylon Bag, and the Nylon Bag of two formed objects is put into the center position of flowerpot;
Step 2, mixes soil and fertilizer in the ratio of 10: 1, make culture matrix, and culture matrix is put in to sterilizing 40min at 121 DEG C, and the culture matrix after sterilizing is filled in Nylon Bag and flowerpot;
Step 3 is broadcast equably 3 crops one in Nylon Bag, and between nylon membrane and flowerpot, 1 crop two is respectively broadcast in the position corresponding with crop one, broadcasts altogether 3, and crop one is identical apart from the beeline of nylon membrane with crop two, and uses permanent pen mark position;
Step 4, after planting covers with ranker, and waters, and growing period management is with conventional;
Step 5, measured the soil sample on rhizosphere soil and the nylon membrane of interculture one, interculture two, nonoculture crop one and nonoculture crop two respectively in crop one and the suitable growth phase of crop two;
The implementation method that soil sample on crop rhizosphere soils and nylon membrane is measured is:
One of the inside Nylon Bag is taken out carefully from flowerpot, take out in the markd position of Nylon Bag, the degree of depth that fetches earth is 10-15cm;
Vertically cut off along mark with scissors carefully at outside Nylon Bag, get the soil sample in Nylon Bag outside at its 10-15cm place;
Respectively soil sample is taken back to laboratory with the paper bag of sterilizing, at room-dry, measure respectively the biomass of Soil Microorganism by flat band method, every basin 12 parts of soil samplings altogether, Fababean Intercropping, intercropping wheat, only make broad bean and only make each 3 parts of wheat;
Step 6, analyzes edaphon crop by soil sample measurement result, determines whether crop one and crop two look good with plantation.
2. the method for claim 1, it is characterized in that, in step 1, according to the shape of flowerpot and internal diameter, be that the nylon membrane of 30 microns is curling by aperture, diameter equals respectively 1/2 of the upper and lower interior diameter of flowerpot, by interface nylon line suture, and nylon membrane is done at the bottom of one, bottom is corresponding with flowerpot bottom, flowerpot is large mouthful of handle, and Nylon Bag is also made large mouthful of handle, the Nylon Bag of two formed objects is put into the center position of flowerpot.
3. the method for claim 1, is characterized in that, in step 2, culture matrix after sterilizing is first filled in the tubbiness Nylon Bag in the inside, recharges flowerpot, make Nylon Bag be positioned at the center of flowerpot, and inside and outside soil height is consistent, and lower than the height of Nylon Bag.
4. the method for claim 1, it is characterized in that, when selected crop one is wheat, when crop two is broad bean, in Nylon Bag, broadcast equably 3 wheats, between nylon membrane and basin, 1 broad bean is respectively broadcast in the position corresponding with wheat, broadcasts altogether 3, wheat is identical apart from the beeline of nylon membrane with broad bean, and uses permanent pen mark position;
After planting cover with ranker, and water, growing period management is with conventional;
Respectively the soil sample on Fababean Intercropping, intercropping wheat, nonoculture broad bean and nonoculture wheat rhizosphere soil and nylon membrane is measured in the pod bearing period of broad bean and the shooting stage of wheat.
5. the method for claim 1, is characterized in that, the performing step of the flat band method of the biomass of described mensuration Soil Microorganism is:
Indoor every part of air-dry soil sample is smash to powdered, take 1 gram of dry ground and put into the triangular flask of 20 milliliters that 9 milliliters of sterile purified waters are housed, hand 10 minutes, microbial cell is fully disperseed, obtain 10-1 soil dilution;
Draw 1 milliliter of 10-1 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-2 soil dilution;
Draw 1 milliliter of 10-2 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-3 soil dilution;
Draw 1 milliliter of 10-3 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-4 soil dilution;
Draw 1 milliliter of 10-4 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-5 soil dilution;
Draw 1 milliliter of 10-5 soil dilution in the triangular flask of 20 milliliters of 9 milliliters of sterile purified waters is housed with pipettor, fully mix, obtain 10-6 soil dilution;
Detect actinomycetic quantity with 10-3,10-4,10-5,10-6 dilution 1 milliliter of dull and stereotyped bacterial detection amount of painting beef extract-peptone agar medium, potato agar culture medium flat plate detection fungi amount and starch ammonium salt culture medium flat plate respectively, the flat-plate inverted coating is placed in the incubator of 28 DEG C and cultivates, within 5-10 days, record afterwards the clump count on flat board, and calculate contained bacterium amount in every gram of soil by optimal extension rate.
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| CN104855073A (en) * | 2015-04-21 | 2015-08-26 | 柳州易农科技有限公司 | Companion planting method of crops |
| CN104885737A (en) * | 2015-05-12 | 2015-09-09 | 甘肃省农业科学院旱地农业研究所 | Cultivation method for dry farming entire-membrane covering furrow planting potato and bean intercropping |
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