CN105606769A - Plant root culture and observation apparatus with thickness-variable medium layer - Google Patents
Plant root culture and observation apparatus with thickness-variable medium layer Download PDFInfo
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- CN105606769A CN105606769A CN201610024761.XA CN201610024761A CN105606769A CN 105606769 A CN105606769 A CN 105606769A CN 201610024761 A CN201610024761 A CN 201610024761A CN 105606769 A CN105606769 A CN 105606769A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0001—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00 by organoleptic means
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a plant root culture and observation apparatus with thickness-variable medium layer. The apparatus is of an impermeable trough structure consisting of a U-shaped support, a front transparent plate and a back transparent plate by means of internal gluing. The U-shaped support is provided with a bottom side edge. Outer sides of the front transparent plate and the back transparent plate are both provided with detachable light shields. A detachable partition is arranged in the middle of the apparatus from front to back. A root flattening distribution space with thickness-variable medium can be formed by the partition, thereby facilitating observation, measurement or image acquisition of plant roots and over-ground parts. Medium filled once can be utilized for plant culture and observation repeatedly; specifically, for the first time, the upper layer of medium is filled after sowing at a fixed height; since then, users sow seeds at different height under the surface of the medium with special seeders. Uniform water supplement to the medium is achieved by a mlicro drip irrigation method utilizing multiple drip emitters arranged side by side before frequent sowing.
Description
Technical field
The invention belongs to plant or crops experimental technique field, be specifically related to a kind of hypothallus variable thicknessRoot system of plant cultivate and observation device.
Background technology
Plant anchors in soil or other matrix by root system, absorbs moisture and mineral nutrient, root system withRhizosphere microorganism exists and interacts. Modern biology research shows that plant root is synthetic and to overground part transmissionHormone or other adjustment signals, very important in aspect performances such as growth and development of plants, environment-stress responsesEffect (Perez etc., 2011). Except water planting or adopt artificial transparent medium, root system of plant is distributed inIn opaque soil or other matrix, can not directly visually observe or obtain visible images,The spatial distribution in soil by special device or equipment observation root system, observes root growth dynamic, isOne of important content of phytobiology and agronomy research.
In soil or other culture matrixes, the observation of root system mainly contains two kinds of approach, and the first is to plant growth groundRoot system in some soil, in-situ root system is observed; It two is in specific device, to cultivate plant and carry out rootThe observation of system. The former is commonly called in-situ root system observation, mainly contains three class methods, and the first is to buildEnter the Large Underground root chamber of formula, by transparent glass window, root system of plant is observed; The second is to excavate soilEarth section is also installed transparent windows, and the growth of reclaimed soil relief plant recovery, carries out root system from observation windowObservation, for example " a kind of method of dynamically researching in-situ root system " (CN101574044A); The third isIn soil, bore and dig peephole and imbed lucite pipe (being mini-rhizotron), obtain and manage with special scanning meansThe root system image of sub-surrounding is also analyzed, for example RhizoScan in-situ root system scanner (Cheng, W.,D.C.ColemanandJ.E.Box,1991MeasuringrootturnoverusingtheminirhizotronTechnique.AgricultureEcosystems&Environment34:261 – 267.). This method is to rootSystem and soil have disturbance, may need just can carry out scientific observation after convalescence of 6 months-1 year(Johnson,M.G.,D.T.Tingey,D.L.PhillipsandM.J.Storm,2001Advancingfinerootresearchwithminirhizotrons.Environmental&ExperimentalBotany45:263-289.). Rear a kind of approach is to design and produce in advance root system observation device, inserts soil or other matrix, trainingAfter supporting plant, carry out root system observation, be generally referred to as " root case method ".
According to the difference in Plant size to be measured and test period, the shape that root case method can flexible design deviceShape and size are arranged test in the environment such as manually operated greenhouse, with large-scale chamber or in-situ root system scanningInstrument is compared, and its cost is relatively low, also not to root system and Soil Surrounding disturbance, avoids recovering growth and waits forProcess and with the inconsistent phenomenon of reset condition. Another advantage of root case method is to obtain root system sample than wildOuter excavation is more convenient. But the weak point of root case method is that artificial container is in limitations, fillingSoil or matrix and field or farmland natural soils between have larger difference. Similar with root case method, canCultivate with the earth culture or the matrix that adopt pvc pipe or other tubular containers to carry out plant, tested rear taking-upAfter earth pillar, water obtains root system sample after rinsing, for example, have a kind of utility model patent to announce employing transparent pipeSub and openable photomask can repeatedly carry out root system observation in process of the test(CN203661692U), separately have 1 patent application of having announced propose to adopt cylindrical container and inThe concentric nylon wire cylinder of portion forms the experimental rig of planting plants, is convenient to obtain Rhizosphere Soil and non-Rhizosphere Soil sample(CN103293035A)。
Even if in the root case manually designing and producing, the root system being distributed in soil or other matrix still can notVisually observe or measure, adopt X ray three-dimensional imaging, computed tomography (CT) or nuclear magnetic resonanceThe methods such as imaging technique can obtain the distributed in three dimensions information of root system, but have apparatus expensive and space size etc.Restriction. Another kind of solution route is soil or matrix to have been rinsed out while test, and acquisition root system sample carries outMeasure or obtain image analysis, but the method can not obtain the original distribution characteristics of root system. The third sideMethod in the soil or dielectric layer of flattening, makes planting after control planting location and root case slant settingObtain in the shallow-layer matrix of more Root Distribution after glass plate, be commonly referred to as root system of plant plane and cultivate sightSurvey method, related to this, contribute to obtain more complete root system image by fluorescence report gene(Rellan-Alvarez,R.,G.Lobet,H.Lindner,P.L.Pradier,J.Sebastianetal.,2015GLO-Roots:animagingplatformenablingmultidimensionalcharacterizationofsoil-Grownrootsystems.Elife4.); Between seed fastening glass panels and paper material (LeMarie,C.,N.Kirchgessner,D.Marschall,A.WalterandA.Hund,2014Rhizoslides:paper-basedgrowthsystemfornon-destructive,highthroughputphenotypingofrootDevelopmentbymeansofimageanalysis.PlantMethods10:13.), allow more points of root systemsCloth is convenient to observe in a plane, and this extreme plane root system observation procedure can obtain more clearlyRoot system Two dimensional Distribution image, but may have very big-difference with the nature that root system forms. In addition, alsoUtilize transparent agar gel or bead to serve as culture medium, be convenient to naked-eye observation root system or picked-up planeOr the two dimensional image of multi-angle (Topp, C.N., A.S.Iyer-Pascuzzi, J.T.Anderson, C.R.Lee, P.R.Zureketal.,20133Dphenotypingandquantitativetraitlocusmappingidentifycoreregionsofthericegenomecontrollingrootarchitecture.ProcNatlAcadSciUSA110:E1695-1704.), synthesize distributed in three dimensions by computer, its shortcoming is culture medium and soilIn the media such as difference is large, gel, easily grow microorganism, be generally only applicable to the short-period test in seedling stage, lightAccording to being had, suppresses or other not clear impacts root growth. Someone proposes detachable plants root box design, adoptsNet sheet carries out interior separation only for the convenient sampling to rhizosphere soil, for maize root system and rhizosphere microorganism(Chinese Patent Application No.: 200710069993.8), root system can pass net sheet can not form dividing of root system in testDistrict distributes, and can not realize the object that root system flattening distributes and directly observes by transparent window.
In sum, existing root system of plant culture vessel has the Root Distribution space of fixed size, notCan form the hypothallus of variable thickness, can not form the flattening of root system in the hypothallus of variable thickness and distributeAnd repeatedly observe or pickup image by detachable shading plate and transparent windows.
Summary of the invention
The object of the invention is to overcome the deficiency that above-mentioned prior art exists, provide a kind of hypothallus thickness canThe root system of plant becoming is cultivated and observation device; Be specifically related to the square culture tank with front and back transparent windows,Form the variable root system flattening distribution space of stromal thickness, be convenient to root system of plant and overground part to seeExamine, measure or obtain image.
The object of the invention is to be achieved through the following technical solutions:
The root system of plant that the present invention relates to a kind of hypothallus variable thickness is cultivated and observation device, and described device hasGlue together by U-shaped support and front transparent panel, the rear transparent panel with bottom side the non-leakage groove forming through insideBody structure; Described front transparent panel, rear transparent panel outside are provided with and can load and unload shadow shield; Front and back along described device are darkThe centre position of degree is also provided with dismountable dividing plate.
Preferably, described dismountable dividing plate by detachable hang the side-hung formula at the side of described deviceSupport is fixed.
Preferably, described dismountable dividing plate is fixed by bending support; Described bending support removablyBe arranged on described bottom of device, or be removably suspended on the edge at described device top.
Preferably, the base of described U-shaped support and side leave respectively the flanging of 0.8～1.2cm, and top is stayedThere is the top margin of 2～3cm.
Preferably, in described U-shaped support, each flanging is respectively equipped with stainless steel parting bead near angle; Described UThe contact position of type support, stainless steel parting bead and transparent panel is fixing with silica adhesive gummed.
Preferably, in transparent panel outside, on the framework between flanging and the top margin of U-shaped support, be equipped with gap ditchGroove; Describedly load and unload that shadow shield is nested to be arranged in the groove of described gap. Gap groove on described top margin is to pass throughLead to, can load and unload shadow shield through the gap groove on top margin, the nested gap groove that is arranged on base, sideIn.
Preferably, described device also comprises seeder, and described seeder is by the stainless steel tube of 8～12mm diameterWith reinforcing bar T font thimble composition, the diameter of described thimble is compared with the little 1～1.5mm of the diameter of stainless steel tube; DescribedWhen thimble inserts stainless steel tube completely, slightly expose on top.
Preferably, described stainless steel tube outside is provided with graduation mark.
Preferably, described device also comprises micro drip-irrigation equipment; Described micro drip-irrigation equipment is by connecting water tapAdapter, arm and some arrow compositions.
Preferably, the speed of dropping water of described arrow in order to make in device, dry wet basis matter line of demarcation is per hour moves down 5～6cm。
Compared with prior art, the present invention has following beneficial effect:
1) utilization of the present invention is with the root system of plant observation groove of front and back transparency glass plate, set up plant individual cultivate,The method of root system observation and image acquisition. From root case method was different in the past, the technology can realize a cultivationThe hypothallus of 2 variable thickness before and after forming in groove, more plant is planted in front and back simultaneously on the one hand, on the other handRealize root system flattening in various degree and distributed, be applicable to the plant individual of different plant species, different growth phases,Can carry out observation, measurement or the pickup image of root system and overground part simultaneously;
2) utilize device of the present invention, in the viewing test such as Root Distribution and mesocotyl prolongation of rice seedlingIn, can realize the contrasts such as different soils water content, different cultivars, different depths of planting and process, at seedOn the character observations such as sprouting is emerged, root growth and mesocotyl prolongation, obtain good implementation result, obtainRoot system image can be measured by analysis software the experimental datas such as root length.
Brief description of the drawings
Fig. 1 is that root system of plant is cultivated and observation device schematic diagram;
Fig. 2 is for can load and unload shadow shield schematic diagram;
Fig. 3 adopts the plastic foamboard of different-thickness or the overlapping and specific support of polylith to culture tank inner chamberCarry out the partition of fore-and-aft direction, form the schematic diagram of variable hypothallus thickness, wherein, a is side-hung formulaSupport, b is top bending type support;
Fig. 4 is the medium grain type Seeder structural representations such as rice wheat;
Fig. 5 is the schematic diagram that supplements water supply in media with micro drip-irrigation, and wherein, a is that multiple arrows distribute side by side and doHygroscopic water boundary line, b is for dripping arrow and connecting branch;
Fig. 6 is that the seed of two rice varieties (C1, C4) is at the soil of three kinds of water content (S1, S2, S3)Live rear germination and emergence situation in earth;
Fig. 7 is that rice paddy seed live rear seedlings root in the soil of three kinds of water content (S6, S7, S8) distributesSituation;
Fig. 8 be different rice varieties seedling mesocotyl lengths and on seed deeply emerge impact observation schematic diagram;
In figure, 1 is stainless steel bottom side support, and 2 is transparency glass plate, and 3 is silica adhesive, and 4 is flanging,5 is top margin, and 6 for can load and unload shadow shield, and 7 is the region that bends inwards, shadow shield top, and 8 is stainless steel parting bead,9 is monolithic plastic foamboard, and 10 is polylith plastic foamboard, and 11 is medium space, and 12 is suspended scaffold,13 is bending support, and 14 is stainless steel tube, and 15 is reinforcing bar T font thimble, and 16 is scale label line, 17For dripping arrow, 18 for dripping arrow flow rate switch, and 19 is dry hygroscopic water boundary line.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in detail. Following examples will contribute to the skill of this areaArt personnel further understand the present invention, but do not limit in any form the present invention. It should be pointed out that thisThe those of ordinary skill in field, without departing from the inventive concept of the premise, can also make some tuneWhole and improve. These all belong to protection scope of the present invention.
Embodiment 1
The present embodiment relates to a kind of root system of plant of hypothallus variable thickness and cultivates and observation device. As Fig. 1 instituteShow, device (root system is cultivated and observation groove) is by stainless steel bottom side support 1 and front and back transparency glass plate 2 warpsInner gummed forms, and glass plate outside is equipped with can load and unload shadow shield 6 (Fig. 2), by the dividing plate of multi-thicknessInner space is cut apart, after filling substrate, formed the hypothallus of different-thickness.
In the present embodiment, stainless steel bottom side support 1 by the stainless steel materials of 0.5mm thickness through cutting,Bend and be welded, being U font; This stainless steel stent leaves the flanging of 1cm left and right at base and side4, on top, leave the connection top margin 5 about 2.5cm, for improving firmness and the stability of support.
In the present embodiment, the interior each flanging of stainless steel stent 1 is thick near angle placement 1cm width 2mmDegree stainless steel parting bead 8 (a), the clear glass flat board 2 of 5mm thickness is placed respectively in front and back to Fig. 3, support-everyFixing with silica adhesive 3 between bar-glass plate, be specially at glass plate and stainless steel stent gap and knuckleLocate even silicon-coating viscose binder 3, all sealing gap, forms non-leakage square cell body, i.e. rootSystem cultivates and observation groove. This root system is cultivated and observation groove forms approximately between glass plate and flanging 4 and top margin 5The gap groove of 3mm, utilizes groove that the stainless steel shadow shield 6 of 2mm thickness can be installed. Stainless steel hidesThe top of tabula rasa 6 10mm that bends inwards, the region 7 that bends inwards, shadow shield top shelters from glass plate topLight, make shadow shield keep straight and be convenient to charge and discharge operations simultaneously.
When the root system of the present embodiment is cultivated and observation groove is used for the medium plant type crops such as water paddy and wheat class, can adoptDegree of depth equal-specification before and after 40cm width, 60cm height and 13.5cm. For other crops or plant testTime can adjust according to plant and root system range size the concrete size of root system observation groove. Because root system is cultivated and observationBefore and after groove, be transparency glass plate, can sow vegetable seeds at front and back glass plate and matrix intersection simultaneously, sameIn a cell body, cultivate more plant, compare mutually in close environmental condition.
Soil can all be filled in the inner space that the root system of the present embodiment is cultivated between the glass plate of observation groove front and backEarth or other matrix, form the hypothallus of the full degree of depth, is applicable to the strain phase test of the crop such as paddy rice, wheat;Can also insert from the centre position of the front and back degree of depth monolithic 9 of different-thickness or the polylith of stack 10 hard bubblesFoam plastic plate (Fig. 3) forms the medium space 11 of different-thickness between cystosepiment and glass plate, inserts trainingAfter foster matrix, form the hypothallus of different-thickness, along with block board thickness increases, the thickness of hypothallus lowers, forPlant seedling stage or compared with the test of plantlet strain phase; Thickness regulate hypothallus can meet Individual Size difference,The needs that the different plant individual of growth phase is grown, the abundant flattening that can realize again different big or small root systems dividesCloth. This rigid foam plate also can adopt the solid slab of other materials or hollow frame to replace. Concrete nextSay that the polylith cystosepiment of monolithic or stack or other sheet material can adopt side-hung cribbing 12 to carry outFixing. Can also adopt the bending support 13 at bottom and top to be fixed.
The root system of the present embodiment is cultivated and is observed the hypothallus in groove repeatedly sow and seedling for vegetable seedsGrowth. When recycling hypothallus carries out sowing for the second time or repeatedly, need to adopt custom-designed seeder (figure4) seed is sown into the following different degree of depth of stromal surface. This seeder, by the stainless steel tube of 10mm diameter14 and the reinforcing bar T font thimble 15 of 9mm diameter form, after thimble inserts stainless steel tube completely, low side slightly revealsGo out and point slightly, can be about 100mm from T-handle to most advanced and sophisticated distance. This stainless steel tube and T shape thimbleCan adopt other materials to make, change its diameter and length according to seed size and depth of planting scope. This is notRust steel pipe outside starts to draw scale label line 16 from top, when sowing, seeder is inserted in matrix and reaches pre-After fixed depth of planting scale, extract T-handle out, seed is dropped in pipe and make it fall into bottom, detachLoosening periphery matrix when pipe, covers hole and completes sowing.
The root system of the present embodiment is cultivated and observation groove, after a front plant cultivation and observation experiment finish, and canAfter taking out matrix, refill, also can retain matrix repeatedly for plant cultivation and observation. Sowing first can be adoptedWith filling substrate extremely both take the altitude, after planting again load the method for top layer matrix. Thereafter recycling is filledWhen hypothallus, take out original plant, placing a few days is to make withered brownization of residual root system, adopts micro drip-irrigation method(Fig. 5) matrix is evenly kept the skin wet. Micro drip-irrigation is by the adapter that connects water tap, arm and drip an arrow17 compositions (can buy from micro drip-irrigation equipment supplier). In the hypothallus of 40cm width, can evenly arrange7-9 of row drip arrow, regulate flow rate switch 18 to make to drip arrow and reach desired flow, require each arrow speed of dropping water baseThis is consistent, does not cause the matrix sinking of subsiding in drip irrigation process. This micro drip-irrigation flow velocity can be adjusted to dry wet basis matter and divideBoundary line 19 5-6cm that moves down per hour.
Following examples 2～4 are shown in concrete application:
Embodiment 2
Different soils water content affects the viewing test of direct-sowing dry rice seedling
Enough planting soils are rolled, sieved, remove stone and other impurity, airing one week on concrete floorIt is for subsequent use that left and right forms even dry ground. Take out appropriate evenly dry ground and add water while stirring, repeated multiple times until moundBottom starts to ooze out moisture, obtains the soil S1 that approaches saturation moisture capacity; Separate half S1 soil, and greatlyApproximately isopyknic dry ground stirs and evenly mixs repeatedly, obtains soil S2; Separate half S2 soil, with about body that waitsLong-pending dry ground mixes, and obtains soil S3. Each mound covered with plastic film spends the night, and makes the interior moisture of mound abundantBalance, obtains the soil that 3 kinds of water content present graded. Drying weight method is measured, soil moisture contentPresent close to linear graded (Fig. 6) from 27% left and right to 16% left and right.
Utilize the plant that the present invention proposes to cultivate and root system observational technique, place 2 at two ends, culture tank bottomBending type support, at the overlapping cystosepiment of 2 2.7cm thickness of central closing insertion, 2 of top left-hand threadsBending type support fixed foam Board position is filled three kinds of S1, S2, S3 etc. between cystosepiment and glass plateThe soil of different moisture content.
The seed of two rice varieties (C1, C4) was soaked after 12 hours in running water, be seeded in trainingSupport soil table following 3cm place in groove, make seed press close to glass plate, light barrier is inserted in outside, and culture tank is placed onIn the open company's hot house of surrounding, germination, remove light barrier after emerging 10 days, picked-up seedling andThe photo of root system, observes, analyzes the germination of live seed in different moisture content soil, the situation of emerging (figure6). Can see too weight in wet base of S1 soil, but seed can germinate emerge not freely, and root growth is slow;S2 soil moisture content is moderate, and seed sprouting is neat, root volume and degree of depth maximum; S3 soil is too dry,Seed is germination and emergence normally, and C1 kind has small and weak root system.
Embodiment 3
The viewing test of rice seedling root length and distribution in different moisture content soil
With reference to the method for embodiment 2, obtain the soil sample (S6, S7, S8) of 3 kinds of different moisture contents,Drying method is measured water content and is respectively 26.5%, 23.5% and 20% (Fig. 7), is filled into root system observationIn groove. Below soil table, 8cm place sowing rice paddy seed, makes seed press close to glass plate, and stainless steel is installed in both sidesShadow shield, in the open hot house of surrounding, shading is cultivated 10 days. After removing sunshade net and shadow shield, absorbImage viewing Root Distribution situation (Fig. 7), can see in the soil of 26.5% water content rice seedlingRoot growth be suppressed, with another two compared with compared with the soil sample of low water content, the maximum of root system is darkDegree, root length, lateral root number etc. significantly reduce (Fig. 7).
Embodiment 4
Direct-sowing dry rice seedling mesocotyl extends situation and the viewing test of emerging and acting on is seed deeplyed in promotion
With reference to the method for embodiment 2,3, in culture tank, load the soil of proper moisture content (23% left and right)Earth, selects the rice varieties " Shanghai drought 15 " that mesocotyl do not extend, the rice varieties " Yun Lu that mesocotyl extendsNo. 8 " (can buy or obtain from Shanghai City Agricultural biological Gene Center's Germplasm Bank from relevant seeds company). Seed is sown into soil and shows 8cm place, make seed press close to glass plate, both sides are installed stainless steel and are in the lightPlate, multilayer low-transmittance sunshade net is added a cover at top, and in the open hot house of surrounding, shading is cultivated 10 days.Remove pickup image after sunshade net and light barrier, observe that mesocotyl length and seedling plumule, blade are unearthed andGrowing state (Fig. 8).
The mesocotyl significant prolongation of high-visible rice varieties in photo " No. 8, Yun Lu " seedling, broadcasts at 8cmPlanting the degree of depth is that plumule and blade tip stretch out soil table, and the mesocotyl of " Shanghai drought 15 " extends hardly, is seeded inWhen the following 8cm of soil table, whole seedling can not emerge (Fig. 8).
Above specific embodiments of the invention are described. It will be appreciated that, the present invention is not limited toAbove-mentioned specific implementations, those skilled in the art can make within the scope of the claims various distortion or repairChange, this does not affect flesh and blood of the present invention.
Claims (10)
1. the root system of plant of hypothallus variable thickness is cultivated and an observation device, it is characterized in that described dressPut to have by the U-shaped support of bottom side and front transparent panel, rear transparent panel and glue together the non-leakage groove forming through insideBody structure; Described front transparent panel, rear transparent panel outside are respectively equipped with and can load and unload shadow shield; Before described deviceThe centre position of the rear degree of depth is also provided with dismountable dividing plate.
2. the root system of plant of hypothallus variable thickness according to claim 1 is cultivated and observation device, itsBe characterised in that, described dismountable dividing plate is the side-hung cribbing at described device side by detachable hangBe fixed.
3. the root system of plant of hypothallus variable thickness according to claim 1 is cultivated and observation device, itsBe characterised in that, described dismountable dividing plate is fixed by bending support; Described bending support is removably establishedPut in described bottom of device, or be removably suspended on the edge at described device top.
4. the root system of plant of hypothallus variable thickness according to claim 1 is cultivated and observation device, itsBe characterised in that, the base of described U-shaped support and side leave respectively the flanging of 0.8～1.2cm, and top is leftThe top margin of 2～3cm.
5. the root system of plant of hypothallus variable thickness according to claim 4 is cultivated and observation device, itsBe characterised in that, in described U-shaped support, each flanging is respectively equipped with stainless steel parting bead near angle; Described U-shapedThe contact position of support, stainless steel parting bead and transparent panel is fixing with silica adhesive gummed.
6. the root system of plant of hypothallus variable thickness according to claim 4 is cultivated and observation device, itsBe characterised in that, in transparent panel outside, on the framework between flanging and the top margin of U-shaped support, be equipped with gap groove;Describedly load and unload that shadow shield is nested to be arranged in the groove of described gap.
7. the root system of plant of hypothallus variable thickness according to claim 1 is cultivated and observation device, itsBe characterised in that, described device also comprises seeder, described seeder by the stainless steel tube of 8～12mm diameter andReinforcing bar T font thimble composition, the diameter of described thimble is compared with the little 1～1.5mm of the diameter of stainless steel tube; Described topWhen pin inserts stainless steel tube completely, slightly expose on top.
8. the root system of plant of hypothallus variable thickness according to claim 7 is cultivated and observation device, itsBe characterised in that, described stainless steel tube outside is provided with graduation mark.
9. the root system of plant of hypothallus variable thickness according to claim 1 is cultivated and observation device, itsBe characterised in that, described device also comprises micro drip-irrigation equipment; Described micro drip-irrigation equipment is by connecting the suitable of water tapOrchestration, arm and some arrow compositions.
10. the root system of plant of hypothallus variable thickness according to claim 9 is cultivated and observation device, itsBeing characterised in that, in order to make in device, dry wet basis matter line of demarcation is per hour moves down 5 to the speed of dropping water of described arrow～6cm。
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234061A (en) * | 2016-10-10 | 2016-12-21 | 上海泽泉科技股份有限公司 | Culture apparatus and measuring method for high flux plant phenotype imaging |
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