CN107027536A - A kind of method for monitoring indoor culture plant root growth - Google Patents
A kind of method for monitoring indoor culture plant root growth Download PDFInfo
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- CN107027536A CN107027536A CN201710253110.2A CN201710253110A CN107027536A CN 107027536 A CN107027536 A CN 107027536A CN 201710253110 A CN201710253110 A CN 201710253110A CN 107027536 A CN107027536 A CN 107027536A
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- transparent pipes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
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Abstract
A kind of method for monitoring indoor culture plant root growth, comprises the following steps：1. foam plastic reservoir and PC transparent pipes are chosen, PC transparent pipes are one side closed, a side opening；2. two circular ports are opened in foam plastic reservoir one side, PC transparent pipes is put into two circular ports of foamed plastics body side by the inside level of blind end, and blind end is fixed on offside；3. the partially enclosed of casing is stretched out into PC transparent pipes openend, makes pipe iuuminting rate close to 0；4. foam plastic reservoir bottom half diagonally opens up multiple permeable holes；5. vermiculite or sandy soil are poured into foam plastic reservoir as culture matrix, thickness of earth-fill cover is higher than PC transparent pipes in case；6. institute's measuring plants seed or brood body are planted in incubator, cultivated 1~2 month, when root system to be formed presses close to growth on the outside of PC transparent pipes, you can observed.Edaphic condition and observation of plant root growth situation can be controlled by this method in room conditions.
Description
Technical field
The invention belongs to plant research field, it is related to a kind of method for monitoring indoor culture plant root growth.
Background technology
Root system is the important Functional tissue of plant, and only the growth of plant does not provide support, and with absorption, fortune
Water delivery point and the function of inorganic salts, are the passages for contacting plant and soil.Radicula is in the circulation of ecosystem matter and energy
Play an important role.In forest ecosystem, 20%~80% net productivity is contributed by fine root production, and radicula
It is the important component of carbon cycle.Breathing, production, decomposition and the turnover of root system are raised by gas concentration lwevel, greenhouse effects
And the influence of nitrogen deposition, and then influence the material circulation of the world's ecosystems.Therefore, root system research is in widespread attention, respectively
State scientific research personnel in terms of the apparatus derivatorius of different root systems of plant, form, biomass to having carried out extensive work.
In plant growing study, people pay close attention to the dynamic of aerial part for a long time, and ignore the research of underground root system.
This in soil, causes sampling difficulty mainly due to root growth.Therefore, traditional research method is more based on excavation.Soil
Core method (Sequential core) excavates the soil of certain volume using instruments such as root brills, so as to obtain the root system of the inside.Recklessly
Little Ning etc. (2010) is drilled in the locust tree root system that most deep 2m is excavated in Loess Plateau using internal diameter 6.8cm roots.Achat (2008) etc.
Using grooving (10cm × 10cm) and the method for core, 80cm root systems vertically divide with have studied pine tree (Pinus pinaster) sample
Cloth feature, it is believed that can be reduced using the method for grooving and use error.
On the basis of core method, the conventional interior growth method (Ingrowth core) of researcher carrys out dynamic studies root system
Growth course.Liu Jinliang etc. (2009) uses the method, is bored using root after coring, the root system in soil sample is cleared,
Then fill out back, sampled in Second Year in same place, thus evaluate fertilising to Manchurian ash (Fraxinus mandshurica) and
The influence of larch (Larix gmelinii) fine root production.Ostonen etc. (2005) has found what is obtained using interior growth method
Norway spruce (Picea abies) radicula Annual net primary productivity only has the 38.5% of soil drilling method.Because the soil filled out back changes
The property of original soil, quality is softer, and water-retaining property is strong, and gas permeability is strong, is conducive to root growth.To avoid this problem,
The soil that Lukac and Godbold (2001) wraps up same quality using nylon wire changes core, so as to obtain every time as core
Obtain the increment of radicula in intervals.
Biomass method (mining method, root bore method, interior growth method) simple and fast, cost are low, are research root production and week
The common method turned.When boring method using root, research error is larger, is influenceed in sampling process by topography and geomorphology, and for root
The ununified standard of the specification of brill, in sampling process, it sometimes appear that situation (Gan Zhuoting and Liu Wenzhao without radicula
2008；De Silva et al.1999).When studying root growth speed using interior growth method, the soil physical chemistry not only backfilled
Matter changes, and root system is destroyed, and upgrowth situation changes.
In recent years, mini-rhizotron technology is widely applied in root system research.The technology comes from eighties of last century the forties earliest,
Researcher is embedded to transparent plastic or glass in soil, for observing growth of the root system on its surface.With photoelectron technology
Development, this method improved, and the root system for being attached at pipeline outer wall can be scanned using camera, by monitor or is made video recording
Band is recorded in real time, and the root system hologram of formation is used for data analysis.Shi Jianwei etc. (2007) is bent to water using mini-rhizotron technology
Willow and the research discovery of larch fine root production, larch radicula year increment (0.94mmcm-2) and year mortality
(0.72mmcm-2) is significantly lower than Manchurian ash (1.52mmcm-2 and 1.21mmcm-2).Noguchi et al.(2011)
Influence to Japanese cypress radicula productivity is sparselyed fell using mini-rhizotron technical research, it is found that 35% thinning intensity reduction diameter is less than
1mm radicula plant productivity 15.9%.
Mini-rhizotron method is a kind of non-destructive observation, is capable of the upgrowth situation of directly monitoring root system, has been widely used
In external survey out of office and field experiment, and its research development indoors is less.Indoor quantitative control experiment is needed in water
Divide and the metastable interior of temperature, by carrying out the processing such as salt, arid or fertilising, and then observation of plant root system in the medium
Growth conditions under different soil.Compared with field experiment, such experiment can obtain more accurate data, illustrate
Plant root growth mechanism, has great importance in scientific research.On the other hand, in kinds such as crops, vegetables, nursery stocks
In facility cultivation, it is also desirable to which its root system is monitored in real time, to determine and adjust the supply of its liquid manure.However, suitable due to lacking
Measurement apparatus and rational measuring method, researcher is perplexed in the measurement of root system of plant always under environment indoors.Therefore, design
It is a kind of to can be used for indoor progress, easy plant root growth monitoring method, all there is weight to root system research and agricultural production
Want meaning.
The content of the invention
The purpose of the present invention is that a kind of method for monitoring indoor culture plant root growth of offer, passes through the party
Method can control edaphic condition and observation of plant root growth situation in room conditions.
The present invention is to be achieved through the following technical solutions：
A kind of method for monitoring indoor culture plant root growth, comprises the following steps：
1. foam plastic reservoir and PC transparent pipes are chosen, PC transparent pipes are one side closed, a side opening；
2. two circular ports are opened in foam plastic reservoir one side, its aperture is identical with PC transparent pipe external diameters, by PC transparent pipes
Put two circular ports of foamed plastics body side into by the inside level of blind end, and blind end is fixed on offside；
3. the partially enclosed of casing is stretched out into PC transparent pipes openend, makes pipe iuuminting rate close to 0；
4. foam plastic reservoir bottom half diagonally opens up multiple permeable holes；
5. vermiculite or sandy soil are poured into foam plastic reservoir as culture matrix, thickness of earth-fill cover is higher than PC transparent pipes in case；
6. institute's measuring plants seed or brood body are planted in incubator, cultivated 1~2 month, it is saturating that root system to be formed presses close to PC
When being grown on the outside of bright pipe, you can observed.
The foam plastic reservoir is expandable polystyrene material, the outer 350~780cm of path length of casing, wide 270~550cm,
High 200~350cm, 13~25mm of wall thickness.
Transparent 70~the 100cm of pipe range of PC, 4~5mm of wall thickness, 70~80mm of internal diameter, 75~85mm of external diameter.
Two circular port centers being formed with foamed plastics casing one side are at a distance of more than 100mm, apart from foam plastic
Expect bottom half 1/4~2/3, aperture is 75~85mm.
The part that foamed plastics casing is stretched out in the PC transparent pipes openend winds 4~5 layers of black adhesive plaster, makes in its pipe thoroughly
Light rate is close to 0.
The foamed plastics casing bottom diagonally opens up 3~5mm permeable holes 5.
Vermiculite or sandy soil are poured into the foam plastic reservoir body as culture matrix, thickness of earth-fill cover is higher than PC transparent pipes in case
10~20cm of the above.
Compared with prior art, the present invention has following beneficial technique effect：
1. using the inventive method in room conditions, you can the physicochemical property of control soil (culture matrix), so as to see
Examine the influence to plant root growth.
2. the present invention in the container that foam plastic reservoir and PC transparent pipes are constituted by adding culture matrix, and passes through application
It is raw that the measures such as salt, moisture or fertilising change length, diameter, surface area of seedlings root etc. in soil property, routine observation container
Long pointer, will not cause damage to plant, easy to be directly perceived, can long term monitoring.
3. the present invention can be widely used in control experiment and installment agriculture growth research.
Brief description of the drawings：
Fig. 1 is the installation diagram of foam plastic reservoir and PC transparent pipes；
Fig. 2 is the side view of foam plastic reservoir；
Fig. 3 is the use state figure of the present invention.
Embodiment
With reference to specific embodiment come the present invention is described in detail, it is described be explanation of the invention rather than
Limit.
A kind of method for monitoring indoor culture plant root growth, comprises the following steps：
1st, the foam plastic reservoir 1 of expandable polystyrene material, the outer path length 570cm of casing, width 424cm, height are chosen
296cm, wall thickness 25mm；Choose high hard intensity acid and alkali-resistance polycarbonate transparent pipe 2, one side closed, a side opening, pipe range
70cm, wall thickness 4mm, internal diameter 70mm, external diameter 74mm, as shown in Figure 1, 2.
2nd, two circular ports 3 are opened in foamed plastics casing one side, circular port center is at a distance of 141mm, apart from foamed plastics
Bottom half 111cm, pore size is 70mm (in the same size with PC pipe external diameters).
The 3rd, PC transparent pipes are put to two circular ports of foamed plastics body side by the inside level of blind end into, by blind end
Offside is fixed on seccotine, as shown in Figure 1, 2.
4th, the part that foamed plastics casing is stretched out in PC transparent pipes openend is wound 5 layers with black adhesive plaster 4, made in its pipe thoroughly
Light rate is close to 0, as shown in Figure 1, 2.
5th, 3mm permeable holes 5 are diagonally opened up in foamed plastics casing bottom.
6th, 6kg vermiculites are poured into foam plastic reservoir body, as culture matrix, thickness of earth-fill cover is higher than in case more than PC pipes
20cm。
7th, institute's measuring plants seed or brood body are planted in incubator, cultivated 1~2 month, it is saturating that root system to be formed presses close to PC
When being grown on the outside of bright pipe, you can observed, as shown in Figure 3.
The specific contrast experiment using the inventive method is designed below, to illustrate the monitoring effect of this method：
[experimental design]
12gNaCl is configured to 300ml solution, in the incubator for pouring into treatment group, mixing thoroughly reaches NaCl contents in matrix
To 0.2%, 3 are repeated；It is another to take 3 incubators as control, add 300ml distilled water.It is daily to use weight method, supplement evaporation
Lost moisture (excessive moisture of casing outflow returns incubator to keep salt stress concentration stable).
By Robinia pseudoacacia Seeds uniform broadcasting in incubator, cultivate 1~2 month, when seedlings root presses close to raw on the outside of PC transparent pipes
When long, you can observed.
Using CI600 root scanners respectively when cultivating 30 days and 90 days, each PC transparent pipes are scanned one by one, acquisition image
Data, using WinRhizo root systems analysis software to graphical analysis, obtains root length, diameter, surface area, volume, tip of a root number
The growth datas such as amount.
The root growth index of table 1
By taking salt-tolerant plant locust tree as an example, using the method for above-mentioned indoor measurement root system of plant, 0.2%NaCl stress pair is probed into
The influence of its root system.When coercing 30 days, compared with the control, the total length of the lower locust tree root system of 0.2%NaCl processing is reduced
35.7%, projected area, total surface area, average diameter, root system volume and tip of a root quantity add 3.8% respectively, 3.9%,
61.7%th, 86.9% and 14.2%；When coercing 90 days, compared with the control, the total length of the lower locust tree root system of 0.2%NaCl processing
Reduce 2.7%, projected area, total surface area, average diameter, root system volume and tip of a root quantity adds 3.9% respectively,
3.9%th, 6.9%, 9.5% and 3.7%.Illustrate that salt stress declines roots oxidizing increase, length increment, Root morphology is gradually
Tend to short and thick, surface area and volume increase are conducive to absorbing more moisture.With the extension of stress time, root system is gradually
The salt environment of adapted soil, root growth gradually recovers, compared with 30 days, when cultivating 90 days, and treatment group according to group difference with reducing.
The experiment shows, by using the special seedling culture apparatus and root system assay method of design, can accurately control
The characteristics such as salinity, moisture and fertilizer in culture matrix processed, and in the case of undamaged, the growth shape of root system is monitored in real time
State, determines the indexs such as root length, diameter, surface area, volume, tip of a root quantity, and method is easy to be directly perceived, and data accurately and reliably, are solved
The problem that root system is monitored during root system research and industrialized agriculture grow during control of having determined is tested, with larger application prospect.
Claims (7)
1. a kind of method for monitoring indoor culture plant root growth, it is characterised in that：Comprise the following steps：
1. foam plastic reservoir and PC transparent pipes are chosen, PC transparent pipes are one side closed, a side opening；
2. two circular ports are opened in foam plastic reservoir one side, its aperture is identical with PC transparent pipe external diameters, by PC transparent pipes by sealing
The inside level of closed end is put at two circular ports of foamed plastics body side, and blind end is fixed on into offside；
3. the partially enclosed of casing is stretched out into PC transparent pipes openend, makes pipe iuuminting rate close to 0；
4. foam plastic reservoir bottom half diagonally opens up multiple permeable holes；
5. vermiculite or sandy soil are poured into foam plastic reservoir as culture matrix, thickness of earth-fill cover is higher than PC transparent pipes in case；
6. institute's measuring plants seed or brood body are planted in incubator, cultivated 1~2 month, root system to be formed presses close to PC transparent pipes
When outside grows, you can observed.
2. a kind of method for monitoring indoor culture plant root growth according to claim 1, it is characterised in that：Institute
Foam plastic reservoir is stated for expandable polystyrene material, 350~780cm of the outer path length of casing, wide 270~550cm, it is high by 200~
350cm, 13~25mm of wall thickness.
3. a kind of method for monitoring indoor culture plant root growth according to claim 1, it is characterised in that：Institute
State the transparent 70~100cm of pipe range of PC, 4~5mm of wall thickness, 70~80mm of internal diameter, 75~85mm of external diameter.
4. a kind of method for monitoring indoor culture plant root growth according to claim 1, it is characterised in that：Institute
Two circular port centers being formed with foamed plastics casing one side are stated at a distance of more than 100mm, apart from foamed plastics casing bottom
1/4~2/3, aperture is 75~85mm.
5. a kind of method for monitoring indoor culture plant root growth according to claim 1, it is characterised in that：Institute
4~5 layers of black adhesive plaster of part winding that foamed plastics casing is stretched out in PC transparent pipes openend are stated, make its pipe iuuminting rate close to 0.
6. a kind of method for monitoring indoor culture plant root growth according to claim 1, it is characterised in that：Institute
State foamed plastics casing bottom and diagonally open up 3~5mm permeable holes 5.
7. a kind of method for monitoring indoor culture plant root growth according to claim 1, it is characterised in that：Institute
State and vermiculite or sandy soil poured into foam plastic reservoir body as culture matrix, thickness of earth-fill cover higher than more than PC transparent pipes in case 10~
20cm。
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| CN201710253110.2A CN107027536A (en) | 2017-04-18 | 2017-04-18 | A kind of method for monitoring indoor culture plant root growth |
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| CN201710253110.2A CN107027536A (en) | 2017-04-18 | 2017-04-18 | A kind of method for monitoring indoor culture plant root growth |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006315A (en) * | 2018-07-30 | 2018-12-18 | 云南省农业科学院甘蔗研究所 | One kind is for monitoring the dynamic method of sugarcane root growth |
| CN111781223A (en) * | 2020-07-14 | 2020-10-16 | 贵州省山地资源研究所 | Method for in-situ observation of fungal hypha morphology |
| CN114924044A (en) * | 2022-06-06 | 2022-08-19 | 百色学院 | Mango root growth monitoring method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203661692U (en) * | 2013-12-24 | 2014-06-25 | 河南省农业科学院植物营养与资源环境研究所 | Crop root growth observing system |
| CN105160584A (en) * | 2015-08-03 | 2015-12-16 | 中国林业科学研究院林业新技术研究所 | Method and equipment system for in situ dynamic monitoring of wetland plant root system |
| CN105606769A (en) * | 2016-01-15 | 2016-05-25 | 上海市农业生物基因中心 | Plant root culture and observation apparatus with thickness-variable medium layer |
-
2017
- 2017-04-18 CN CN201710253110.2A patent/CN107027536A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203661692U (en) * | 2013-12-24 | 2014-06-25 | 河南省农业科学院植物营养与资源环境研究所 | Crop root growth observing system |
| CN105160584A (en) * | 2015-08-03 | 2015-12-16 | 中国林业科学研究院林业新技术研究所 | Method and equipment system for in situ dynamic monitoring of wetland plant root system |
| CN105606769A (en) * | 2016-01-15 | 2016-05-25 | 上海市农业生物基因中心 | Plant root culture and observation apparatus with thickness-variable medium layer |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006315A (en) * | 2018-07-30 | 2018-12-18 | 云南省农业科学院甘蔗研究所 | One kind is for monitoring the dynamic method of sugarcane root growth |
| CN111781223A (en) * | 2020-07-14 | 2020-10-16 | 贵州省山地资源研究所 | Method for in-situ observation of fungal hypha morphology |
| CN114924044A (en) * | 2022-06-06 | 2022-08-19 | 百色学院 | Mango root growth monitoring method |
| CN114924044B (en) * | 2022-06-06 | 2024-01-26 | 百色学院 | Mango root growth monitoring method |
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Application publication date: 20170811 |
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