CN104137761B - A kind of experimental rig and test method thereof tackling vertical obstacle for studying root system - Google Patents

A kind of experimental rig and test method thereof tackling vertical obstacle for studying root system Download PDF

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Publication number
CN104137761B
CN104137761B CN201410363552.9A CN201410363552A CN104137761B CN 104137761 B CN104137761 B CN 104137761B CN 201410363552 A CN201410363552 A CN 201410363552A CN 104137761 B CN104137761 B CN 104137761B
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root system
delamination area
experimental rig
circular hole
tackling
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CN104137761A (en
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吴鹏飞
邹显花
侯晓龙
刘爱琴
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The present invention relates to a kind of experimental rig of tackling vertical obstacle for studying root system, comprise the transparent cylinder container for plant seedling growth, several lamina septum pellucidums are vertically disposed with so that cylindrical vessel interior separation is become multiple delamination area for filling culture matrix in described cylindrical vessel, each interlayer is provided with the through hole being communicated with upper and lower two adjacent delamination area, the cylindrical vessel sidewall corresponding to each delamination area offers instrument connection.The present invention simultaneously also provides a kind of test method tackling vertical obstacle for studying root system.This is used for studying root system, and to tackle the experimental rig overall structure of vertical obstacle simple, cheap, draw materials with easy to make, simulating natural environment, how research root system starts morphophysiology response policy to tackle growth adverse circumstance, especially the ability of test plant root system reply vertical direction solid obstacle, success rate of afforesting under improving Difficult site condition.

Description

A kind of experimental rig and test method thereof tackling vertical obstacle for studying root system
Technical field
The present invention relates to a kind of experimental rig and the test method thereof of tackling vertical obstacle for studying root system, belonging to the research field of afforestation technology under Difficult site condition.
Background technology
In recent years, China's forest coverage increases substantially, and some easier afforestations complete afforestation task substantially.But along with socioeconomic fast development, the mankind are still very serious to the destruction in forest land, define the Difficult site of the large area stone matter such as such as Abandoned Land of Mine, exposed hill, stone pit, Waste Landfill Site.How to improve the afforestation success rate of these Difficult site, increasing green coverage is current forest workers's urgent problem.For Abandoned Land of Mine, the demand of the mankind to mineral resources increases day by day, and inevitably produce vegetation and soil the exploitation of mineral resources and destroy, strip mining transformation, surface collapse, storing up of selecting and purchasing ore deposit discarded object create a large amount of Abandoned Land of Mines.According to Geological And Mineral Resources Departments In Their rough estimates, national mining area adds up damage to land area and reaches 2,880,000 hm 2, every year with about 4.67 ten thousand hm 2speed increase.The series of environmental problems such as Abandoned Land of Mine not only brings heavy metal pollution, water and soil loss, geological disaster hidden danger increase the weight of, also cause afforestation survival rate, storage rate not high, this is mainly owing to usually meeting with the soil solid obstacles such as Shi Zhi in Root-development, cause the nutrient of Root Absorption, moisture cannot upper grown demand contentedly, so that Stand Growth is slow, poor stability, stand quality is low, can not give play to higher benefit.
But, in long-term evolution process, the perception of some plant to soil adverse circumstances such as stone matter obstacles is strong especially, and countermeasure can be started rapidly, comprise morphological plasticity, physiological plasticity, mycorhiza plasticity etc., some plants can also the diameter of radicula in root system, branch angle, interval and steric configuration realize the evading of soil solid obstacle, the countermeasure such as detour by regulating.Visible, how fully to excavate by research the potential ability that root system of plant tackles soil solid obstacle, to success rate of afforesting under raising Difficult site condition, realize Difficult site quick recovering vegetation tool most important theories and practice significance.
Summary of the invention
In view of this, the object of this invention is to provide a kind of experimental rig and the test method thereof of tackling vertical obstacle for studying root system, structure is simple, easily manufactured, simulating natural environment, the ability of researching plant root reply vertical direction solid obstacle, success rate of afforesting under improving Difficult site condition.
The present invention adopts following scheme to realize: a kind of experimental rig of tackling vertical obstacle for studying root system, comprise the transparent cylinder container for plant seedling growth, several lamina septum pellucidums are vertically disposed with so that cylindrical vessel interior separation is become multiple delamination area for filling culture matrix in described cylindrical vessel, each interlayer is provided with the through hole being communicated with upper and lower two adjacent delamination area, the cylindrical vessel sidewall corresponding to each delamination area offers instrument connection.
Further, described cylindrical vessel lateral wall and each instrument connection corresponding position are provided with the tinfoil hidden on instrument connection.
Further, two lamina septum pellucidums are provided with in described cylindrical vessel, first interlayer of arranging from top to bottom and the second interlayer respectively with by inner for cylindrical vessel three delamination area, be respectively arrange from top to bottom first layer region, the second delamination area and the 3rd delamination area.
Further, described first interlayer is provided with a circular hole A near cylindrical vessel madial wall place, described second interlayer is positioned at immediately below described circular hole A and is provided with the diameter circular hole B identical with circular hole A, the diametric(al) at described circular hole B place is also arranged with several diameters circular hole C identical with circular hole B.
Further, described circular hole B and circular hole C is equidistant arrangement in the diametric(al) at its place.
Simultaneously, the present invention also provides a kind of test method tackling vertical obstacle for studying root system, adopt a kind of experimental rig of tackling vertical obstacle for studying root system as above, and carry out according to the following steps: (1) prepares this experimental rig of predetermined quantity and is divided into some batches; (2) in each delamination area of each experimental rig, the culture matrix of identical nutrient is placed with, sowing or the colory plant seed of predetermined quantity of planting or transplanting nursery stock in the delamination area of the top; (3) regularly utilize Type Water Potential Meter to measure the flow of water of different delamination area by instrument connection on each experimental rig, and keep the skin wet or nutrient solution in the timely delamination area on the low side to the flow of water, ensure the identical flow of water of delamination area tool of all experimental rigs; (4) regularly take out a collection of experimental rig, morphophysiology index determining is carried out to the root system in each delamination area, when tackling vertical obstacle to observe root system, penetrates ability and the growth conditions of different through hole smoothly; (5) to record on different batches each experimental rig in each delamination area, the distribution of each through hole root system and growing state.
Further, in described step (4), the method for morphophysiology index determining scans each root system image by digital scanner, and applies image analysis software carries out quantitative analysis to the root system that each delamination area grows; And utilize the physical signs of gas chromatograph to each delamination area growth root system to carry out quantitative analysis.
Compared with prior art, the present invention has following beneficial effect: overall structure of the present invention is simple, cheap, draw materials with easy to make, simulating natural environment, when root system of plant meets with the solid obstacles such as stone matter, slag, plastic products discarded object in ground vertical-growth process, how research root system starts morphophysiology response policy to tackle this growth adverse circumstance, especially the ability of test plant root system reply vertical direction solid obstacle, success rate of afforesting under improving Difficult site condition.
Accompanying drawing explanation
Fig. 1 is embodiment of the present invention organigram;
Number in the figure illustrates: 1-cylindrical vessel, 2-instrument connection, 3-first interlayer, 4-second interlayer, 5-first layer region, 6-second delamination area, 7-the 3rd delamination area, 8-circular hole A, 9-circular hole B, 10-circular hole C.
Embodiment
For making object of the present invention, technical scheme and advantage clearly understand, below by specific embodiment and relevant drawings, the present invention will be described in further detail.
As shown in Figure 1, a kind of experimental rig of tackling vertical obstacle for studying root system, comprise the transparent cylinder container 1 for plant seedling growth, several lamina septum pellucidums are vertically disposed with so that cylindrical vessel interior separation is become multiple delamination area for filling culture matrix in described cylindrical vessel 1, described cylindrical vessel 1 and interlayer adopt the fluid-tight hard plate material of printing opacity (as glass etc.) to make, to simulate the soil solid obstacle met with in root system vertical-growth process.Each interlayer is provided be communicated with upper and lower two adjacent delamination area with the through hole of root system free penetrating, cylindrical vessel sidewall corresponding to each delamination area offers instrument connection 2, for observing root growth situation, acquisition test sample and keeping the skin wet or nutrient solution.
In the present embodiment, described cylindrical vessel 1 lateral wall and each instrument connection 2 corresponding position are provided with the tinfoil hidden on instrument connection, the loss divided to prevent water, and each instrument connection 2 is positioned at the center in corresponding delamination area vertical direction.
In the present embodiment, two lamina septum pellucidums are provided with in described cylindrical vessel, first interlayer 3 of arranging from top to bottom and the second interlayer 4 respectively with by inner for cylindrical vessel three delamination area, first layer region 5, second delamination area 6 and the 3rd delamination area 7 of arranging from top to bottom respectively, the growing state of root system in each delamination area of observational study, especially after research root system suffers the impact of vertical obstacle, the symmetry of the uniformity that root system distributes in each delamination area of the different soils degree of depth and the position relative to stem.
In the present embodiment, described first interlayer 3 is provided with a circular hole A(8 near cylindrical vessel 1 madial wall place), described second interlayer 4 is positioned at described circular hole A(8) immediately below be provided with a diameter and circular hole A(8) identical circular hole B(9), described circular hole B(9) place diametric(al) on be also arranged with several diameters and circular hole B(9) identical circular hole C(10); Described through hole on first interlayer is circular hole A, and the described through hole on the second interlayer is circular hole B and circular hole C; Wherein circular hole A is used for the space of form or the different sizes formed between solid obstacle in simulated soil, for researching plant root Vertical dimension grow to first layer region 5 time, when part root system meets with the obstruction of the first interlayer, whether this part root system can assemble growth by starting Morphology And Physiology countermeasure to circular hole A, thus evades or obstacle that first interlayer that detoured is formed; After root system enters the second delamination area 6 through circular hole A, we need study root system by circular hole B Vertical dimension grow plant a colony the degree of depth, also have, need to study root system and plant a colony range in the second delamination area, whether the direction of growth of namely assembling through the root system of circular hole A obtains adjustment, the Spatial Distribution Pattern of root system is made to be systematicness, symmetry relative to the position of stem, thus by assembling equably apart from some circular hole C of circular hole B different distance and growing to the 3rd delamination area; The growing state of the above-mentioned root system of comprehensive analysis can disclose the morphophysiology strategy that root system of plant tackles vertical obstacle.
In the present embodiment, described circular hole B(9) and circular hole C(10) be equidistant arrangement in the diametric(al) at its place, pass the uniformity of the second interlayer 4 for studying root system in observation second delamination area 6.
As shown in Figure 1, a kind of test method tackling vertical obstacle for studying root system, comprise a kind of experimental rig of tackling vertical obstacle for studying root system as above, and carry out according to the following steps: (1) prepares this experimental rig of predetermined quantity and is divided into some batches; (2) in each delamination area of each experimental rig, the culture matrix of identical nutrient is placed with, sowing sowing or the colory plant seed of predetermined quantity of planting (as pine and cypress seed etc.) or transplanting nursery stock (as pine and cypress nursery stock etc.) in the delamination area of the top; (3) regularly utilize Type Water Potential Meter to measure the flow of water of different delamination area by instrument connection on each experimental rig, and keep the skin wet or nutrient solution in the timely delamination area on the low side to the flow of water, ensure the identical flow of water of delamination area tool of all experimental rigs; Test and supplement complete employing tinfoil and testing window is sealed, the loss divided to prevent water; (4) regularly take out a collection of experimental rig, morphophysiology index determining is carried out to the root system in each delamination area, when tackling vertical obstacle to observe root system, penetrates ability and the growth conditions of different through hole smoothly; (5) to record on different batches each experimental rig in each delamination area, the distribution of each through hole root system and growing state.
In the present embodiment, in described step (4), the method for morphophysiology index determining scans each root system image by digital scanner, and applies image analysis software carries out quantitative analysis to the root system that each delamination area grows; And utilize the physical signs (as endogenous hormones, organic acid etc.) of gas chromatograph to each delamination area growth root system to carry out quantitative analysis.
Above-listed preferred embodiment; the object, technical solutions and advantages of the present invention are further described; be understood that; the foregoing is only preferred embodiment of the present invention; not in order to limit the present invention; within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (7)

1. tackle the experimental rig of vertical obstacle for studying root system for one kind, it is characterized in that: comprise the transparent cylinder container for plant seedling growth, several lamina septum pellucidums are vertically disposed with so that cylindrical vessel interior separation is become multiple delamination area for filling culture matrix in described cylindrical vessel, each interlayer is provided with the through hole being communicated with upper and lower two adjacent delamination area, the cylindrical vessel sidewall corresponding to each delamination area offers instrument connection.
2. experimental rig of tackling vertical obstacle for studying root system according to claim 1, is characterized in that: described cylindrical vessel lateral wall and each instrument connection corresponding position are provided with the tinfoil hidden on instrument connection.
3. experimental rig of tackling vertical obstacle for studying root system according to claim 1 and 2, it is characterized in that: in described cylindrical vessel, be provided with two lamina septum pellucidums, be respectively first interlayer of arranging from top to bottom with the second interlayer so that cylindrical vessel interior separation is become three delamination area, be respectively arrange from top to bottom first layer region, the second delamination area and the 3rd delamination area.
4. experimental rig of tackling vertical obstacle for studying root system according to claim 3, it is characterized in that: described first interlayer is provided with a circular hole A near cylindrical vessel madial wall place, described second interlayer is positioned at immediately below described circular hole A and is provided with the diameter circular hole B identical with circular hole A, the diametric(al) at described circular hole B place is also arranged with several diameters circular hole C identical with circular hole B.
5. experimental rig of tackling vertical obstacle for studying root system according to claim 4, is characterized in that: described circular hole B and circular hole C is equidistant arrangement in the diametric(al) at its place.
6. tackle the test method of vertical obstacle for studying root system for one kind, it is characterized in that: described in employing claim 1 ~ 5, any one tackles the experimental rig of vertical obstacle for studying root system, and carries out according to the following steps: (1) prepares this experimental rig of predetermined quantity and is divided into some batches; (2) in each delamination area of each experimental rig, the culture matrix of identical nutrient is placed with, sowing or the colory plant seed of predetermined quantity of planting or transplanting nursery stock in the delamination area of the top; (3) regularly utilize Type Water Potential Meter to measure the flow of water of different delamination area by instrument connection on each experimental rig, and keep the skin wet or nutrient solution in the timely delamination area on the low side to the flow of water, ensure the identical flow of water of delamination area tool of all experimental rigs; (4) regularly take out a collection of experimental rig, morphophysiology index determining is carried out to the root system in each delamination area, when tackling vertical obstacle to observe root system, penetrates ability and the growth conditions of different through hole smoothly; (5) to record on different batches each experimental rig in each delamination area, the distribution of each through hole root system and growing state.
7. the test method tackling vertical obstacle for studying root system according to claim 6, it is characterized in that: in described step (4), the method for morphophysiology index determining scans each root system image by digital scanner, and applies image analysis software carries out quantitative analysis to the root system that each delamination area grows; And utilize the physical signs of gas chromatograph to each delamination area growth root system to carry out quantitative analysis.
CN201410363552.9A 2014-07-29 2014-07-29 A kind of experimental rig and test method thereof tackling vertical obstacle for studying root system Expired - Fee Related CN104137761B (en)

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CN104871792A (en) * 2015-06-11 2015-09-02 东北农业大学 Rod bank for testing plant root growth penetrating power
CN105403569A (en) * 2015-12-21 2016-03-16 福建农林大学 Device and method for evaluating the vertical growth avoidance of plant roots from pollutants
CN106769166A (en) * 2016-12-06 2017-05-31 河海大学 A kind of experimental provision for the fine Stratified Sampling of green plant rhizosphere soil
CN108605570A (en) * 2017-10-31 2018-10-02 鞠林 A kind of root system net sheet type forming method
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