CN105403569A - Device and method for evaluating the vertical growth avoidance of plant roots from pollutants - Google Patents
Device and method for evaluating the vertical growth avoidance of plant roots from pollutants Download PDFInfo
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- CN105403569A CN105403569A CN201510966992.8A CN201510966992A CN105403569A CN 105403569 A CN105403569 A CN 105403569A CN 201510966992 A CN201510966992 A CN 201510966992A CN 105403569 A CN105403569 A CN 105403569A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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Abstract
The invention relates to a device for evaluating the vertical growth avoidance of plant roots from pollutants. The device comprises a housing, separators, guide rails, a scanning device and an actuator. The housing includes a base and a sidewall disposed on the base; the base and the side wall form the housing into a cavity with an opening; the separators are disposed in the housing and in the number of two or more; the separators are provided with through holes; the through holes are internally provided with an agar layer; two or more of the separators are respectively disposed on the side wall along a direction from the base to the opening and separate the cavity into more than two closed sub cavities; the guide rails are disposed outside the housing along the circumferential direction of the side wall; the scanning device is slidably disposed on the guide rails; and the actuator drives the scanning device to slide along the guide rails. The invention also relates to a method for evaluating the vertical growth avoidance of plant roots from pollutants. The evaluation apparatus and method of the present invention also have the advantages of significant improvement on test reproducibility and sensitivity of the monitoring.
Description
Technical field
The present invention relates to ecological pollution average evaluation technology, particularly a kind of evaluate plant roots system device and method that pollutant vertical-growth is avoided.
Background technology
Along with expanding economy, in the middle of increasing pollutant emission to environment, wherein final home to return to is soil, and too much pollution makes the Quality Down of soil, and ecological functions reduce.Particularly have the soil of nearly twenty percent to be polluted at present in China, the pollutant in soil, serious threat is to the normal growth of plant, and pollutant first from root system accumulation, and is delivered in whole plant body.And numerous research shows, the root system organ that pollutant accumulation amount is maximum often is also the most serious organ that is injured.But, plant is in the middle of continuous evolutionary process, define the feature strong especially to the perception that the environment-stress such as soil pollution are stronger, once perceive the existence that pollutant etc. coerces, root system of plant can respond fast, start certain adaptation management, these countermeasures comprise root physiological plasticity, root morphological plasticity, and mycorhiza plasticity inside and outside root etc., moreover, some are to the plant of environment-stress sensitivity, often can to fine diameter size in root system, interval from, crotch angle and steric configuration have been come measures such as the avoidances of soil pollutant, with the injury of pollution abatement thing to root system.Therefore, fully disclose and excavate root system of plant to the test system of soil pollutant and potential ability, to recovery, the practical basis that tool is important and the scientific meaning of vegetation under raising soil pollution serious condition.
Application number be 201110345647.4 application for a patent for invention disclose a kind of test unit looking for foster behavior for studying root system vertical direction, the out cylinder comprising the center drum for transplanting plant nursery stock and be nested with one heart outside center drum, region between described center drum and out cylinder is disposed with several horizontal interlayers in the axial direction from bottom to top, region between described center drum and out cylinder is radially laid with several vertical interlayers, to be separated out several for placing the sector region of the Nutrient patches of Different Nutrients, the corresponding tapping hole be laid with towards each sector region on out cylinder perisporium, the barrel of described center drum and out cylinder, horizontal interlayer and vertical interlayer are glass fiber mesh, described glass fibre is coated with agar on the net, the flowing profit intercepting nutrient in different sector region while passing through glass fiber mesh to allow root system of plant utilizes this test unit can closest under natural state, research plant rhizosphere Nutrient time loss totally after, its root system from the close-by examples to those far off responds to the ability opening up new Nutrient patches.But said apparatus cannot obtain root system tri-dimensional picture, there is the problem of the insufficient sensitivity of monitoring.
Summary of the invention
Technical matters to be solved by this invention is: the device that the evaluate plant roots system providing a kind of monitoring sensitivity high avoids pollutant vertical-growth, further for the method that said apparatus provides evaluate plant roots system to avoid pollutant vertical-growth.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is:
The device that a kind of evaluate plant roots system avoids pollutant vertical-growth, comprise housing, separator, guide rail, scanning device and actuator, described housing comprises substrate and is arranged at suprabasil sidewall, described substrate and sidewall make housing form the cavity volume with opening, described separator is arranged in housing and number is two or more, described separator is provided with through hole, agar layer is provided with in described through hole, plural described separator is arranged on sidewall and by described cavity volume along substrate to opening direction respectively and is separated to form the airtight sub-cavity volume of two or more, described guide rail is arranged at outside housing along sidewall peripheral direction, described scanning device is slidably arranged on guide rail, described actuator drives scanning device along slide.
The present invention also discloses a kind of method that evaluate plant roots system avoids pollutant vertical-growth, comprise the device that above-mentioned evaluate plant roots system avoids pollutant vertical-growth, and comprise the following steps: soil or plant nutrition liquid are put into plural described sub-cavity volume respectively, then in plural described sub-cavity volume, place the pollutant of variable concentrations respectively, the root system of plant to be tested is disperseed and stretches in plural described sub-cavity volume respectively, drive scanning device along slide by actuator, thus scanning obtains the distributed intelligence of root system of plant to be tested in housing.
Beneficial effect of the present invention is:
By comprising the structural design of housing, separator, guide rail, scanning device and actuator, actuator drives scanning device along slide, thus scan and obtain the distributed intelligence of root system of plant more fully to be tested in housing (tri-dimensional picture), and then to observe more fully and under analyzing the condition of the pollutant heterogeneity that do not coexist, the growth of the footpath level of different sub-cavity volume root system and distribution major parameter situation, avoid situation with evaluate plant roots system to the vertical-growth of pollutant; Its device one-piece construction is easy, and the simple and convenient operation of method step, significantly improves the sensitivity of test reappearance and monitoring simultaneously, have broad application prospects in contaminated soil ecological risk Study and estimate.
Accompanying drawing explanation
Fig. 1 is that the evaluate plant roots system of the embodiment of the present invention is to the one-piece construction schematic diagram of the device that pollutant vertical-growth is avoided.
Label declaration:
1, housing; 11, substrate; 12, sidewall; 2, separator; 3, guide rail; 4, scanning device; 5, actuator; 6, sub-cavity volume; 7, controller; 8, data output apparatus; 9, cover; 10, plant to be tested.
Embodiment
By describing technology contents of the present invention in detail, realized object and effect, accompanying drawing is coordinated to be explained below in conjunction with embodiment.
The design of most critical of the present invention is: by comprising the structural design of housing, separator, guide rail, scanning device and actuator, obtain the distributed intelligence of root system of plant more fully to be tested, and then observe more fully and analyze the growth of the footpath level of different sub-cavity volume root system and the major parameter situation that distributes, improve monitoring sensitivity with this.
Please refer to Fig. 1, the device that a kind of evaluate plant roots system of the present invention avoids pollutant vertical-growth, comprise housing 1, separator 2, guide rail 3, scanning device 4 and actuator 5, described housing 1 comprises substrate 11 and is arranged at suprabasil sidewall 12, described substrate 11 and sidewall 12 make housing 1 form the cavity volume with opening, described separator 2 is arranged in housing 1 and number is two or more, described separator 2 is provided with through hole, agar layer is provided with in described through hole, plural described separator 2 is arranged on sidewall 12 and by described cavity volume along substrate 11 to opening direction respectively and is separated to form the airtight sub-cavity volume 6 of two or more, described guide rail 3 is arranged at outside housing 1 along sidewall 12 peripheral direction, described scanning device 4 is slidably arranged on guide rail 3, described actuator 5 drives scanning device 4 to slide along guide rail 3.
The present invention also discloses a kind of method that evaluate plant roots system avoids pollutant vertical-growth, comprise the device that above-mentioned evaluate plant roots system avoids pollutant vertical-growth, and comprise the following steps: soil or plant nutrition liquid are put into respectively plural described sub-cavity volume 6, then in plural described sub-cavity volume 6, place the pollutant of variable concentrations respectively, the root system of plant 10 to be tested is disperseed and stretches in plural described sub-cavity volume 6 respectively, scanning device 4 is driven to slide along guide rail 3 by actuator 5, thus scanning obtains the distributed intelligence of plant 10 root system to be tested in housing 1.
From foregoing description, beneficial effect of the present invention is:
By comprising the structural design of housing, separator, guide rail, scanning device and actuator, actuator drives scanning device along slide, thus scan and obtain the distributed intelligence of root system of plant more fully to be tested in housing (tri-dimensional picture), and then to observe more fully and under analyzing the condition of the pollutant heterogeneity that do not coexist, the growth of the footpath level of different sub-cavity volume root system and distribution major parameter situation, avoid situation with evaluate plant roots system to the vertical-growth of pollutant; Its device one-piece construction is easy, and the simple and convenient operation of method step, significantly improves the sensitivity of test reappearance and monitoring simultaneously, have broad application prospects in contaminated soil ecological risk Study and estimate.
In the device that a kind of evaluate plant roots system of the present invention avoids pollutant vertical-growth:
Further, also comprise converter, controller 7 and data output apparatus 8, described converter is electrically connected with scanning device 4, and described converter, controller 7 and data output apparatus 8 are electrically connected respectively successively.Described converter is USB converter, and described data output apparatus 8 is computing machine, and described scanning device 7 is optical scanner, and described actuator 5 is motor.
Seen from the above description, said structure design makes scanning device scan the tri-dimensional picture information obtained and is converted to data message by converter, transfer in data output apparatus by controller again, then by the operation of many data output apparatus, carry out three-dimensional reconstruction, finally carry out 3-d modelling, wherein, in research process, under emphasis observes the condition of the pollutant heterogeneity that do not coexist, the growth of the footpath level of different locellus root system and distribution major parameter situation.Concrete, converter is USB converter, and data output apparatus is computing machine, and scanning device is optical scanner, and actuator is motor.
Further, described separator 2 is reticulate texture, and described agar layer is arranged in the mesh on cancellated described separator 2.
Seen from the above description, separator can be reticulate texture, and agar layer is arranged in mesh, root system can be grown by different sub-cavity volumes, and pollutant can not move.
Further, the material of described separator 2 is nylon.
Seen from the above description, the material of separator is preferably nylon material, has the good and erosion resistant advantage of intensity.
Further, the number of described sub-cavity volume 6 is 3-5.
Seen from the above description, the number of sub-cavity volume is preferably 3-5, in sub-cavity volume, correspondingly place the pollutant of 3-5 variable concentrations.
Further, the material of described housing 1 is transparent material.
Seen from the above description, the material of housing is preferably transparent material, as clear glass, plastics etc., has convenient advantage of observing.
Further, also comprise cover 9, it is outside that described cover 9 covers at housing 1, and the color of described cover 9 is dark.
Seen from the above description, dark cover has good light-shading effect.The material of cover can be any material, such as common plastics, glass, silica gel etc.The color of cover is dark, and described dark color refers to the low color of the colour purities such as black, mazarine, brown, bottle green or cyan.
Please refer to Fig. 1, embodiments of the invention one are:
The device that a kind of evaluate plant roots system of the present invention avoids pollutant vertical-growth, comprise housing 1, separator 2, guide rail 3, scanning device 4, actuator 5, converter, controller 7, data output apparatus 8 and cover 9, it is outside that described cover 9 covers at housing 1, and the color of described cover 9 is black.Described housing 1 comprises substrate 11 and is arranged at suprabasil sidewall 12, and housing 1 is high 100cm and the right cylinder of diameter 50cm, and its material is clear glass, and be convenient to direct vision root growth situation, cover 9 covers housing 1 when root system of plant normal growth.Described substrate 11 and sidewall 12 make housing 1 form the cavity volume with opening, described separator 2 is arranged in housing 1 and number is two or more, its shape is reticulate texture, material is nylon, agar layer is provided with in mesh on cancellated described separator 2, root system of plant and moisture can be passed through, and nutrient and pollutant not by.Plural described separator 2 is arranged on along substrate 11 to opening direction respectively and is separated to form 3 airtight sub-cavity volumes 6 on sidewall 12 and by described cavity volume, and concrete, sub-cavity volume can vertically, be separated every 15-30cm.Described guide rail 3 by housing base to opening direction, be arranged at outside housing 1 along sidewall 12 peripheral direction around ground, described scanning device 4 is slidably arranged on guide rail 3, described actuator 5 drives scanning device 4 to slide along guide rail 3, namely provides power to make optical scanner do 360 ° of rotations around housing.Described converter is electrically connected with scanning device 4, and described converter, controller 7 and data output apparatus 8 are electrically connected respectively successively.Described converter is USB converter, and described data output apparatus 8 is computing machine, and described scanning device 7 is optical scanner, and described actuator 5 is motor.Concrete is: optical scanner is for scanning the root system situation in different sub-cavity volume, and by scanning to obtain root system tri-dimensional picture, by USB converter providing power, simultaneously by USB wiring connection control device and computing machine, the 3D rendering of root system is gone out, for the distributed intelligence in the vertical direction of in-situ scanning root system by the Software on Drawing in computing machine.Plant to be tested is placed in the middle of the housing with different sub-cavity volume, root system of plant to be tested is divided into several part equably.Root system of plant to be tested is distributed in each sub-cavity volume uniformly.And nutrient solution or soil are inserted sub-cavity volume.Under the drive of motor, optical scanner is around housing 360 ° rotation, and scanning is to obtain root system of plant tri-dimensional picture to be tested, and carry out three-dimensional reconstruction by computer software, finally carry out 3-d modelling, under in research process, emphasis observes the condition of the pollutant heterogeneity that do not coexist, the growth of the footpath level of different locellus root system and distribution major parameter situation.Said apparatus designed by the present invention and method can be used for the avoidance experiment that plant grows in the vertical direction, for in soil pollution situation, root system avlidance behavior response policy is illustrated, for the phytoremediation measure of contaminated site provides foundation by the growing state of home position observation root system.
In sum, evaluate plant roots system provided by the invention has the simply compact advantage of device one-piece construction to the device that pollutant vertical-growth is avoided, its evaluation method has the simple and advantage of convenient operation of method step, in addition, above-mentioned evaluating apparatus and evaluation method have the advantage of the sensitivity significantly improving test reappearance and monitoring simultaneously, have broad application prospects in contaminated soil ecological risk Study and estimate.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every equivalents utilizing instructions of the present invention and accompanying drawing content to do, or be directly or indirectly used in relevant technical field, be all in like manner included in scope of patent protection of the present invention.
Claims (9)
1. evaluate plant roots system device that pollutant vertical-growth is avoided, it is characterized in that, comprise housing, separator, guide rail, scanning device and actuator, described housing comprises substrate and is arranged at suprabasil sidewall, described substrate and sidewall make housing form the cavity volume with opening, described separator is arranged in housing and number is two or more, described separator is provided with through hole, agar layer is provided with in described through hole, plural described separator is arranged on sidewall and by described cavity volume along substrate to opening direction respectively and is separated to form the airtight sub-cavity volume of two or more, described guide rail is arranged at outside housing along sidewall peripheral direction, described scanning device is slidably arranged on guide rail, described actuator drives scanning device along slide.
2. evaluate plant roots system according to claim 1 device that pollutant vertical-growth is avoided, it is characterized in that, also comprise converter, controller and data output apparatus, described converter is electrically connected with scanning device, and described converter, controller and data output apparatus are electrically connected respectively successively.
3. evaluate plant roots system according to claim 2 device that pollutant vertical-growth is avoided, it is characterized in that, described converter is USB converter, and described data output apparatus is computing machine, described scanning device is optical scanner, and described actuator is motor.
4. the device avoided pollutant vertical-growth of evaluate plant roots system according to claim 1, it is characterized in that, described separator is reticulate texture, and described agar layer is arranged in the mesh on cancellated described separator.
5. the device avoided pollutant vertical-growth of evaluate plant roots system according to claim 1, it is characterized in that, the material of described separator is nylon.
6. the device avoided pollutant vertical-growth of evaluate plant roots system according to claim 1, is characterized in that, the number of described sub-cavity volume is 3-5.
7. the device avoided pollutant vertical-growth of evaluate plant roots system according to claim 1, it is characterized in that, the material of described housing is transparent material.
8. the device avoided pollutant vertical-growth of evaluate plant roots system according to claim 1, it is characterized in that, also comprise cover, described cover covers at outside, and the color of described cover is dark.
9. evaluate plant roots system method that pollutant vertical-growth is avoided, it is characterized in that, comprise the device that the evaluate plant roots system described in claim 1-8 any one avoids pollutant vertical-growth, and comprise the following steps: soil or plant nutrition liquid are put into plural described sub-cavity volume respectively, then in plural described sub-cavity volume, place the pollutant of variable concentrations respectively, the root system of plant to be tested is disperseed and stretches in plural described sub-cavity volume respectively, drive scanning device along slide by actuator, thus scanning obtains the distributed intelligence of root system of plant to be tested in housing.
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| CN201510966992.8A CN105403569A (en) | 2015-12-21 | 2015-12-21 | Device and method for evaluating the vertical growth avoidance of plant roots from pollutants |
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| CN201510966992.8A CN105403569A (en) | 2015-12-21 | 2015-12-21 | Device and method for evaluating the vertical growth avoidance of plant roots from pollutants |
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Cited By (7)
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| CN106706880A (en) * | 2016-11-29 | 2017-05-24 | 中国科学院南京土壤研究所 | Springtail avoidance test device and application thereof |
| CN107494015A (en) * | 2017-08-26 | 2017-12-22 | 上海曼斐电器贸易有限公司 | Flowerpot |
| CN108050929A (en) * | 2017-11-16 | 2018-05-18 | 华南农业大学 | A kind of method and system for measuring root system of plant spatial distribution |
| CN110866972A (en) * | 2019-11-12 | 2020-03-06 | 广西大学 | In-situ observation device for sugarcane root system configuration and analysis method thereof |
| CN112858300A (en) * | 2021-01-25 | 2021-05-28 | 西南民族大学 | Method for determining morphological structures of different degradation gradient plant roots of alpine meadow |
| CN113075380A (en) * | 2021-03-04 | 2021-07-06 | 河海大学 | Plant root form multi-angle synchronous observation and environment monitoring experiment system |
| CN114041385A (en) * | 2021-11-15 | 2022-02-15 | 宁波市农业科学研究院 | Research method for root growth and development of vegetable and fruit crops |
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Application publication date: 20160316 |