CN103960038A - Test device and method for researching root competition strategy - Google Patents
Test device and method for researching root competition strategy Download PDFInfo
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- CN103960038A CN103960038A CN201410232847.2A CN201410232847A CN103960038A CN 103960038 A CN103960038 A CN 103960038A CN 201410232847 A CN201410232847 A CN 201410232847A CN 103960038 A CN103960038 A CN 103960038A
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Abstract
The invention provides a test device and method for researching a root competition strategy. The test device comprises a center cylinder for simulating the root competition strategy of a plant and a concentric cylinder which is concentrically sleeved at the outer side of the center cylinder, wherein a plurality of vertical interlayers are evenly distributed in the area between the center cylinder and the concentric cylinder at the outer layer along the radial direction, so as to form a plurality of sector areas for containing nutrients; and bottom plates are arranged at the bottoms of the center cylinder and the concentric cylinder. The test device disclosed by the invention can research the capacity of a plant of sensing environment change and starting competitive strategy, especially tests the capacity of the plant root systems of expanding and occupying space along with increase of competition intensity to water and fertilizer resources in soil under the simulated nature state, and the test device is simple in overall structure, low in construction cost and convenient in material preparation and fabrication.
Description
Technical field
The present invention relates to the research field of root system of plant competitive behavior, relate generally in the heterogeneous distributional environment of soil resource patch, the research of different plants or kindred plant root competition behavior aspect, a kind of for studying experimental rig and the method for root competition strategy specifically.
Background technology
Because plant cannot move freely in natural environment as animal, in whole life cycle, constantly suffer the competition to total environmental resource in planting and between planting.Therefore, competition is present in all plant communitys, is to determine one of phytomorph, the history of life and structure of plant community and dynamic major impetus.Compared with only light being competed with acrial part, the competition of under ground portion relates to the soil resource such as moisture, mineral matter, and the competition between plant mainly betides between underground root system.Plant is depended on physiology and morphology plasticity, the Spatial Distribution Pattern of root system in soil etc. of the occupied soil space of its root system, root system to the competitive ability of soil moisture and nutrient.But, because root growth is underground, growth rhythm changes complicated, and present height heterogeneity on the distribution space-time of soil moisture and nutrients resource, in Traditional Scientific research process root system observe and sampling rate more difficult, be difficult to study effectively and disclose the competitive strategy of root system of plant in soil.
Summary of the invention
The present invention improves the problems referred to above, the technical problem to be solved in the present invention be in Traditional Scientific research process root system observe and sampling rate more difficult, be difficult to study effectively and disclose the competitive strategy of root system of plant in soil.
Technical program of the present invention lies in: a kind of for studying the experimental rig of root competition strategy, it is characterized in that, comprise for the center drum in simulating plant root competition region and be nested with one heart the concentric drums in center drum outside, region between described center drum and outer concentric drums is radially evenly laid with several vertical interlayers, to be separated out several for placing the sector region of nutrient, the bottom of described center drum and concentric drums is provided with base plate.
Further, described center drum, concentric drums and vertically interlayer all adopt the fluid-tight hard material of printing opacity to make, to intercept different the flowing of root system IPN and nutrient in region of supporting.
Further, all offer testing window on the lateral wall of several sector regions that described concentric drums is separated out, the inwall upside of several sector regions that described concentric drums is separated is out provided with planting tank.
Another technical scheme of the present invention is: a kind of for studying the test method of root competition strategy, comprise as claimed in claim 3 a kind ofly it is characterized in that for studying the experimental rig of root competition strategy, and carry out according to the following steps:
(1) get the colory plant seed of some, plant seed is seeded in the planting tank of above-mentioned experimental rig, and in the center drum of each experimental rig and sector region, be placed with respectively the culture matrix of Different Nutrients, wherein each sector region is supported identical;
(2) the above-mentioned experimental rig that is placed with plant seed and nutrient patch is divided into some batches;
(3) regularly measure after the different flow of water of supporting region of each experimental rig by testing window, adopt tinfoil that testing window is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get root system in each keeping region and carry out Morphology and physiology and learn the mensuration of index, emphasis is observed the competitive strategy of different plants in each experimental rig center drum or kindred plant root system;
(5) record test data: different time sections is respectively supported distribution and the growing state of root system in region.
Further, in described step (3), utilize Type Water Potential Meter to measure after the flow of water in each keeping region by described square tube testing window, keep the skin wet or nutrient solution to supporting in region in time, support the tool identical flow of water in region until different.
Further, in described step (4), get the root system in each keeping region, scan each root system image by digital scanning instrument, applies image analysis software carries out quantitative analysis to the root system of each keeping region growing; Utilize the physiologic index such as the root system secretion organic acid of the instruments such as high performance liquid chromatograph to Competition for Nutrients region to carry out determination and analysis.
Compared with prior art, the present invention has following beneficial effect: experimental rig of the present invention can be under the state of simulating nature, researching plant root is along with soil liquid manure resource contention intensity is continued to increase, its root system perception environmental change the ability, especially the test plant root system that start competitive strategy are expanded the ability of capturing space.Its overall structure is simple, cheap, draws materials with easy to make.
Brief description of the drawings
Fig. 1 is the structural representation of experimental rig of the present invention.
In figure: 1-center drum, 2-concentric drums, 3-vertically interlayer, 4-sector region, 5-base plate, 6-planting tank, 7-testing window.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described in detail.
Be illustrated in figure 1 a kind of embodiment of the present invention, comprise for the center drum in simulating plant root competition region and be nested with one heart the concentric drums 2 in center drum 1 outside, region between center drum 1 and outer concentric drums 2 is radially evenly laid with several vertical interlayers 3, to be separated out several for placing identical nutrient (or different in moisture, below all to place Different Nutrients as example) the sector region 4 of nutrient patch, the bottom of center drum 1 and concentric drums 2 is provided with base plate 5, described center drum 1, concentric drums 2 and vertically all adopt the fluid-tight hard material of printing opacity to make every 3 layers, in the present embodiment, hard material is glass, to intercept different the flowing of root system IPN and nutrient in region of supporting.
In the present embodiment, several vertical interlayers are for having four, and vertically the region between adjacent two cylinders is divided into four sector regions by interlayer.On the lateral wall of several sector regions that concentric drums is separated out, all offer testing window 7, the inwall upside of several sector regions that concentric drums is separated is out provided with planting tank 6, is provided with the planting tank 6 of circular groove shape in the upper surface of center drum.Testing window 7 is positioned at the center in each fan-shaped keeping region, on testing window 7, hides tinfoil, with the loss that prevents that water from dividing.
Another technical scheme of the present invention is: a kind of for studying the test method of root competition strategy, comprise as claimed in claim 3 a kind ofly for studying the experimental rig of root competition strategy, and carry out according to the following steps:
(1) get the colory plant seed of some, plant seed is seeded in the planting tank of above-mentioned experimental rig, and in the center drum of each experimental rig and sector region, be placed with respectively the culture matrix of Different Nutrients, wherein each sector region is supported identical;
(2) the above-mentioned experimental rig that is placed with plant seed and nutrient patch is divided into some batches;
(3) regularly measure after the different flow of water of supporting region of each experimental rig by testing window, adopt tinfoil that testing window is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get root system in each keeping region and carry out Morphology and physiology and learn the mensuration of index, emphasis is observed the competitive strategy of different plants in each experimental rig center drum or kindred plant root system;
(5) record test data: different time sections is respectively supported distribution and the growing state of root system in region.
In above-mentioned steps (3), utilize Type Water Potential Meter to measure after the flow of water in each keeping region by described square tube testing window, keep the skin wet or nutrient solution to supporting in region in time, support the identical flow of water of region tool until different.
In above-mentioned steps (4), get the root system in each keeping region, scan each root system image by digital scanning instrument, applies image analysis software carries out quantitative analysis to the root system of each keeping region growing; Utilize the physiologic index such as the root system secretion organic acid of the instruments such as high performance liquid chromatograph to Competition for Nutrients region to carry out determination and analysis.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (6)
1. one kind for studying the experimental rig of root competition strategy, it is characterized in that, comprise for the center drum in simulating plant root competition region and be nested with one heart the concentric drums in center drum outside, region between described center drum and outer concentric drums is radially evenly laid with several vertical interlayers, to be separated out several for placing the sector region of nutrient, the bottom of described center drum and concentric drums is provided with base plate.
2. according to claim 1 a kind of for studying the experimental rig of root competition strategy, described center drum, concentric drums and vertically interlayer all adopt the fluid-tight hard material of printing opacity to make, to intercept different the flowing of root system IPN and nutrient in region of supporting.
3. according to claim 2 a kind of for studying the experimental rig of root competition strategy, on the lateral wall of several sector regions that described concentric drums is separated out, all offer testing window, the inwall upside of several sector regions that described concentric drums is separated is out provided with planting tank.
4. for studying a test method for root competition strategy, comprise as claimed in claim 3 a kind ofly it is characterized in that for studying the experimental rig of root competition strategy, carry out according to the following steps:
(1) get the colory plant seed of some, plant seed is seeded in the planting tank of above-mentioned experimental rig, and in the center drum of each experimental rig and sector region, be placed with respectively the culture matrix of Different Nutrients, wherein each sector region is supported identical;
(2) the above-mentioned experimental rig that is placed with plant seed and nutrient patch is divided into some batches;
(3) regularly measure after the different flow of water of supporting region of each experimental rig by testing window, adopt tinfoil that testing window is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get root system in each keeping region and carry out Morphology and physiology and learn the mensuration of index, emphasis is observed the competitive strategy of different plants in each experimental rig center drum or kindred plant root system;
(5) record test data: different time sections is respectively supported distribution and the growing state of root system in region.
5. according to claim 4 a kind of for studying the test method of root competition strategy, in described step (3), utilize Type Water Potential Meter to measure after the flow of water in each keeping region by described square tube testing window, keep the skin wet or nutrient solution to supporting in region in time, support the tool identical flow of water in region until different.
6. according to claim 4 a kind of for studying the test method of root competition strategy, in described step (4), get the root system in each keeping region, scan each root system image by digital scanning instrument, applies image analysis software carries out quantitative analysis to the root system of each keeping region growing; Utilize high performance liquid chromatograph to carry out determination and analysis to the root system secretion organic acid physiologic index in Competition for Nutrients region.
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| CN105432351A (en) * | 2015-12-21 | 2016-03-30 | 福建农林大学 | Device and method for testing mutual influences of plants and soil animals in polluted environment |
| CN106718255A (en) * | 2017-03-10 | 2017-05-31 | 广州花云网络科技有限公司 | Plant root growth test container and its method of testing |
| CN107646411A (en) * | 2017-10-17 | 2018-02-02 | 沈阳大学 | A kind of plant root growth experimental provision |
| CN109315177A (en) * | 2018-09-19 | 2019-02-12 | 湖南人文科技学院 | The comparison unit and method of a kind of crop root system to space and resource contention |
| CN109580882A (en) * | 2018-12-06 | 2019-04-05 | 农法自然（上海）农业科技有限公司 | Device and its measuring method of the different fertilizer to same strain plant root effects |
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