CN102369877A - Test device and method for researching on nutrient foraging behavior of root system in vertical direction - Google Patents
Test device and method for researching on nutrient foraging behavior of root system in vertical direction Download PDFInfo
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- CN102369877A CN102369877A CN2011103456474A CN201110345647A CN102369877A CN 102369877 A CN102369877 A CN 102369877A CN 2011103456474 A CN2011103456474 A CN 2011103456474A CN 201110345647 A CN201110345647 A CN 201110345647A CN 102369877 A CN102369877 A CN 102369877A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention relates to a test device and method for researching on the nutrient foraging behavior of a root system in a vertical direction. The device comprises a central cylinder which is used for transplanting a plant seedling and an outer cylinder which is concentrically sheathed on the external side of the central cylinder. The device is characterized in that a plurality of horizontal isolating layers are sequentially arranged in an area between the two concentric cylinders from the bottom to the top along an axis direction and a plurality of vertical isolating layers are arranged in the area between the two concentric cylinders along a radial direction so as to form a plurality of sector areas used for placing nutrient patches with different nutrients, discharge holes which are communicated with the sector areas are correspondingly arranged on the circumferential wall of the outer cylinder, the walls of the two concentric cylinders, the horizontal isolating layers and the vertical isolating layers are fiber glass nets, and agar is coated on the fiber glass nets to ensure that the root system of the plant can successfully penetrate through the fiber glass nets and the nutrients in the different sector areas are prevented from flowing at the same time. By using the test device, the ability of the root system in sensing and developing new nutrient patches from near to far in a state closest to a natural state after rhizosphere nutrients are used up with time can be researched and especially the ability of the root system of the plant in foraging the nutrients can be tested.
Description
Technical field
The present invention relates to the research field that plant looks for the behavior of supporting, relate generally to the research that the root system of plant vertical direction is looked for the behavior of supporting, a kind ofly specifically be used to study experimental rig and the method that the root system vertical direction is looked for the behavior of supporting.
Background technology
Available nutrient of soil content is one of important attribute of the ecosystem, and the soil nutrient special heterogeneity is prevalent in the natural ecosystems, and the space scale of available nutrient of soil content changes greatly, can reach by the scope of plant individual root system perception.Plant in order to obtain soil resource to greatest extent, produces various plasticity reactions to the special heterogeneity of nutrient in the long-term evolution process, comprise morphological plasticity, physiological plasticity, mycorhiza plasticity etc.The root system of many plant speciess is a large amount of hyperplasia in the patch that nutrient enriches, and the hyperplasia degree interspecific difference is bigger, and receive patch attribute (patch size, nutrient density), nutritive element kind and nutrient totally to supply the influence of situation.Plant is also through adjusting the efficient utilization that rich diameter, branch angle, internode distance and steric configuration of supporting radicula in the patch realizes the patch nutrient.
Summary of the invention
The present invention provides a kind of experimental rig and method that the root system vertical direction is looked for the behavior of supporting that be used to study; Utilize this experimental rig can be under state near nature; The research plant is at the rhizosphere nutrient in time behind the approach exhaustion; Its root system is from the close-by examples to those far off responded to the ability of the new nutrient patch of developing, and especially the test plant root system is vertically looked for foster ability.
Technical program of the present invention lies in: a kind ofly be used to study the experimental rig that the root system vertical direction is looked for the behavior of supporting; Comprise and be used to transplant the center drum of plant nursery stock and be nested with outer cylinder with one heart in the center drum outside; It is characterized in that: the zone between said two concentric drums is disposed with several horizontal interlayers from bottom to top along axis direction; Zone between said two concentric drums radially is laid with several vertical interlayers; To be separated out the sector region that several are used to place the nutrient patch of Different Nutrients; The corresponding tapping hole that is laid with towards each sector region on the outer cylinder perisporium; The barrel of said two concentric drums, horizontal interlayer and vertically interlayer be glass fiber mesh, said glass fibre is coated with agar on the net, intercepts flowing of nutrient in the different sector regions when letting root system of plant pass glass fiber mesh smoothly.
As a preferred version, described several horizontal interlayers are 4 horizontal interlayers, and described several vertical interlayers are 4 vertical interlayers, said inside and outside two concentric drums and horizontal interlayer, 16 sector regions of the common formation of vertical interlayer.
As a preferred version, said tapping hole is arranged on the center of each sector region, hides tinfoil on the said tapping hole, with the loss that prevents that water from dividing.
Another technical scheme of the present invention is: a kind of being used to studied the test method that the root system vertical direction is looked for the behavior of supporting, and comprises above-mentioned experimental rig, it is characterized in that, carries out according to the following steps:
(1) get the identical plant nursery stock of growing way of some, the plant nursery stock be moved in the center drum of above-mentioned experimental rig, and in the sector region of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;
(2) the above-mentioned experimental rig that is placed with plant nursery stock and nutrient patch is divided into some batches;
(3) regularly adopt the method for injector to inject in the sector region of each experimental rig, to keep the skin wet or nutrient solution, after injection finishes, adopt tinfoil that tapping hole is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get the mensuration that the interior root system of each sector region carries out physiology and morphological index;
(5) record test data: the distribution and the growing state of root system in each sector region of different time sections.
As a preferred version, above-mentioned steps (4) is for getting the root system of each sector region, and through each root system image of digital scanning appearance scanning, applies image analysis software carries out quantitative analysis to each layer root system, carries out the mensuration of relevant physiological index simultaneously.
The invention has the advantages that: experimental rig of the present invention can be under the most approaching natural state; The research plant is at the rhizosphere nutrient in time behind the approach exhaustion; Its root system is from the close-by examples to those far off responded to the ability of the new nutrient patch of developing, and especially the test plant root system is vertically looked for foster ability.Its overall structure is simple, and is cheap, draws materials with easy to make.
Description of drawings
Fig. 1 is the structural representation of experimental rig of the present invention.
Among the figure: 1-center drum, 2-outer cylinder, 3-horizontal interlayer, 4-vertically interlayers, 5-sector region, 6-tapping hole.
Embodiment
For letting the above-mentioned feature and advantage of the present invention can be more obviously understandable, hereinafter is special lifts embodiment, and conjunction with figs., elaborates as follows.
The structural representation of experimental rig of the present invention as shown in Figure 1, the sidepiece of this figure adopt the mode of perspective to represent, with abundant displaying layer of the present invention shelf structure, the other side is the diagram of profile.
A kind of experimental rig that the root system vertical direction is looked for the behavior of supporting that is used to study of the present invention; Comprise and be used to transplant the center drum 1 of plant nursery stock and be nested with outer cylinder 2 with one heart in the center drum outside; It is characterized in that: the zone between said two concentric drums 1 and 2 is disposed with several horizontal interlayers 3 from bottom to top along axis direction; Zone between said two concentric drums 1 and 2 radially is laid with several vertical interlayers 4; To be separated out the sector region 5 that several are used to place the nutrient patch of Different Nutrients; The corresponding tapping hole 6 that is laid with towards each sector region on outer cylinder 2 perisporiums; The barrel of said two concentric drums, horizontal interlayer 3 and vertically interlayer 4 be glass fiber mesh, said glass fibre is coated with agar on the net, intercepts flowing of nutrient in the different sector regions when letting root system of plant pass glass fiber mesh smoothly.
As a preferred version, described several horizontal interlayers are 4 horizontal interlayers, and described several vertical interlayers are 4 vertical interlayers, said inside and outside two concentric drums and horizontal interlayer, 16 sector regions of the common formation of vertical interlayer.
As a preferred version, said tapping hole is arranged on the center of each sector region, hides tinfoil on the said tapping hole, with the loss that prevents that water from dividing.
A kind of being used to of the present invention studied the test method that the root system vertical direction is looked for the behavior of supporting, and comprises above-mentioned experimental rig, it is characterized in that, carries out according to the following steps:
(1) get the identical plant nursery stock of growing way of some, the plant nursery stock be moved in the center drum 1 of above-mentioned experimental rig, and in the sector region of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;
(2) the above-mentioned experimental rig that is placed with plant nursery stock and nutrient patch is divided into some batches;
(3) regularly adopt the method for injector to inject in the sector region of each experimental rig, to keep the skin wet or nutrient solution, after injection finishes, adopt tinfoil that tapping hole 6 is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get the mensuration that the interior root system of each sector region carries out physiology and morphological index;
(5) record test data: the distribution and the growing state of root system in each sector region of different time sections.
As a preferred version, above-mentioned steps (4) is for getting the root system of each sector region, and through each root system image of digital scanning appearance scanning, applies image analysis software carries out quantitative analysis to each layer root system, carries out the mensuration of relevant physiological index simultaneously.
Embodiment is following more specifically:
The research China fir is for the foster behavior of looking for of P elements: the China fir nursery stock is moved in the sand culture device inner core (non-treatment region) that is made up of two concentric drums, and inner tube wall and interlayer are the fiberglass screen material of aperture 2mm, and China fir main root and fibrous root all can penetrate smoothly.Carry out the heterogeneous phosphorus of soil when handling, in every layer of one of them fan-shaped outer region (treatment region), apply granular long-acting slow-release phosphorus source patch respectively, does not repeat mutually the fan-shaped position of selecting separately between four layers.Sand culture matrix is vanadine (Al
2O
3, cross the 28-48 order) and ratio and quartz sand mixing with 1.5%, need carry out dephosphorization treatment before the dress basin to alumina; Earlier do not have muddiness, soak 24h with 0.01mol/L HCl again, with clear water the alumina surface acid is rinsed well more afterwards with flushing with clean water alumina to water; Soak 24h with 0.01mol/L HCl; So reprocessing is ten times, and is to guarantee the abundant wash-out of the phosphorus in the alumina, air-dry for use.Quartz sand is also used clear water and is cleaned to guarantee the abundant wash-out of its phosphorus.The used matrix of phosphorus source patch is with the KH of variable concentrations
2PO
4Soak and mix sand, utilize vanadine can effectively adsorb the characteristic that phosphorus also can slowly evenly discharge available phosphorus, the control cultivation medium is to the supply of available phosphorus, the KH that applies
2PO
4Earlier use dissolved in distilled water, alumina and solution mixed soaking 24h, the dried sand after the adding quantitatively mixes again.Duration of test carries out dephosphorization element outer normal nutrient solution and water supply; The processing of holing in each fan-shaped center from the experimental rig side; And the method that adopts syringe equivalent to inject; With the supply of moisture or nutrient solution in the guarantee test processing procedure, after finishing, injection utilize tinfoil to hide, with the loss that prevents that water from dividing.After the test processing time finishes; Device is carried out layering and divides patch to handle, get the root system of each fan-shaped patch, through each root system image of digital scanning appearance scanning; Applies image analysis software carries out quantitative analysis to each layer root system, carries out the mensuration of relevant physiological index simultaneously.
The present invention does not limit to above-mentioned preferred forms, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.
Claims (5)
1. one kind is used to study the experimental rig that the root system vertical direction is looked for the behavior of supporting; Comprise and be used to transplant the center drum of plant nursery stock and be nested with outer cylinder with one heart in the center drum outside; It is characterized in that: the zone between said two concentric drums is disposed with several horizontal interlayers from bottom to top along axis direction; Zone between said two concentric drums radially is laid with several vertical interlayers; Being separated out the sector region that several are used to place the nutrient patch of Different Nutrients, the corresponding tapping hole that is laid with towards each sector region on the outer cylinder perisporium, the barrel of said two concentric drums, horizontal interlayer and vertically interlayer be glass fiber mesh; Said glass fibre is coated with agar on the net, intercepts flowing of nutrient in the different sector regions when letting root system of plant pass glass fiber mesh smoothly.
2. a kind of experimental rig that the root system vertical direction is looked for the behavior of supporting that is used to study according to claim 1; It is characterized in that: described several horizontal interlayers are 4 horizontal interlayers; Described several vertical interlayers are 4 vertical interlayers, said inside and outside two concentric drums and horizontal interlayer, 16 sector regions of the common formation of vertical interlayer.
3. a kind of being used to according to claim 1 studied the experimental rig that the root system vertical direction is looked for the behavior of supporting, and it is characterized in that: said tapping hole is arranged on the center of each sector region, hides tinfoil on the said tapping hole, with the loss that prevents that water from dividing.
4. one kind is used to study the test method that the root system vertical direction is looked for the behavior of supporting, and comprises above-mentioned experimental rig, it is characterized in that, carries out according to the following steps:
(1) get the identical plant nursery stock of growing way of some, the plant nursery stock be moved in the center drum of above-mentioned experimental rig, and in the sector region of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;
(2) the above-mentioned experimental rig that is placed with plant nursery stock and nutrient patch is divided into some batches;
(3) regularly adopt the method for injector to inject in the sector region of each experimental rig, to keep the skin wet or nutrient solution, after injection finishes, adopt tinfoil that tapping hole is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get the mensuration that the interior root system of each sector region carries out physiology and morphological index;
(5) record test data: the distribution and the growing state of root system in each sector region of different time sections.
5. a kind of test method that the root system vertical direction is looked for the behavior of supporting that is used to study according to claim 1; It is characterized in that: above-mentioned steps (4) is for getting the root system of each sector region; Through each root system image of digital scanning appearance scanning; Applies image analysis software carries out quantitative analysis to each layer root system, carries out the mensuration of relevant physiological index simultaneously.
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Cited By (12)
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| CN102860227A (en) * | 2012-09-11 | 2013-01-09 | 福建农林大学 | Test device and method used for studying root phenotypic plasticity |
| CN102972231A (en) * | 2012-12-24 | 2013-03-20 | 山东省花生研究所 | Peanut culturing device and system as well as authenticating method for drought resistant peanut varieties |
| CN103960038A (en) * | 2014-05-29 | 2014-08-06 | 福建农林大学 | Test device and method for researching root competition strategy |
| CN104303865A (en) * | 2014-10-28 | 2015-01-28 | 云南省农业科学院农业环境资源研究所 | Method for measuring nutrient competition of kindred plants under water planting |
| CN104429854A (en) * | 2014-11-27 | 2015-03-25 | 福建农林大学 | Testing device and method for studying how root system reacts to local intermittent pulse nourishing environment |
| CN105165454A (en) * | 2015-09-23 | 2015-12-23 | 天津大学 | Immersed multicompartment submerged plant rhizosphere box |
| CN105403569A (en) * | 2015-12-21 | 2016-03-16 | 福建农林大学 | Device and method for evaluating the vertical growth avoidance of plant roots from pollutants |
| CN105432351A (en) * | 2015-12-21 | 2016-03-30 | 福建农林大学 | Device and method for testing mutual influences of plants and soil animals in polluted environment |
| CN105875392A (en) * | 2016-06-03 | 2016-08-24 | 中铁西北科学研究院有限公司 | Auxiliary device for plant growing and water retaining in arid region |
| CN108513830A (en) * | 2018-07-02 | 2018-09-11 | 贵州大学 | Experimental provision for the research of root system of plant foraging behavior |
| CN108925281A (en) * | 2018-07-30 | 2018-12-04 | 安徽农业大学 | For analyzing the potting bucket for heating and influencing on wheat root distribution |
| CN114041385A (en) * | 2021-11-15 | 2022-02-15 | 宁波市农业科学研究院 | Research method for root growth and development of vegetable and fruit crops |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860227A (en) * | 2012-09-11 | 2013-01-09 | 福建农林大学 | Test device and method used for studying root phenotypic plasticity |
| CN102972231A (en) * | 2012-12-24 | 2013-03-20 | 山东省花生研究所 | Peanut culturing device and system as well as authenticating method for drought resistant peanut varieties |
| CN103960038A (en) * | 2014-05-29 | 2014-08-06 | 福建农林大学 | Test device and method for researching root competition strategy |
| CN104303865B (en) * | 2014-10-28 | 2016-08-17 | 云南省农业科学院农业环境资源研究所 | The method measuring kindred plant Competition for Nutrients relation under the conditions of water planting |
| CN104303865A (en) * | 2014-10-28 | 2015-01-28 | 云南省农业科学院农业环境资源研究所 | Method for measuring nutrient competition of kindred plants under water planting |
| CN104429854A (en) * | 2014-11-27 | 2015-03-25 | 福建农林大学 | Testing device and method for studying how root system reacts to local intermittent pulse nourishing environment |
| CN105165454A (en) * | 2015-09-23 | 2015-12-23 | 天津大学 | Immersed multicompartment submerged plant rhizosphere box |
| CN105403569A (en) * | 2015-12-21 | 2016-03-16 | 福建农林大学 | Device and method for evaluating the vertical growth avoidance of plant roots from pollutants |
| CN105432351A (en) * | 2015-12-21 | 2016-03-30 | 福建农林大学 | Device and method for testing mutual influences of plants and soil animals in polluted environment |
| CN105875392A (en) * | 2016-06-03 | 2016-08-24 | 中铁西北科学研究院有限公司 | Auxiliary device for plant growing and water retaining in arid region |
| CN108513830A (en) * | 2018-07-02 | 2018-09-11 | 贵州大学 | Experimental provision for the research of root system of plant foraging behavior |
| CN108925281A (en) * | 2018-07-30 | 2018-12-04 | 安徽农业大学 | For analyzing the potting bucket for heating and influencing on wheat root distribution |
| CN114041385A (en) * | 2021-11-15 | 2022-02-15 | 宁波市农业科学研究院 | Research method for root growth and development of vegetable and fruit crops |
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