CN105165454A - Immersed multicompartment submerged plant rhizosphere box - Google Patents
Immersed multicompartment submerged plant rhizosphere box Download PDFInfo
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- CN105165454A CN105165454A CN201510617828.6A CN201510617828A CN105165454A CN 105165454 A CN105165454 A CN 105165454A CN 201510617828 A CN201510617828 A CN 201510617828A CN 105165454 A CN105165454 A CN 105165454A
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- rhizosphere
- room
- root
- sidewall
- chamber
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
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- Environmental Sciences (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses an immersed multicompartment submerged plant rhizosphere box which comprises a root chamber, a rhizosphere chamber and a non-rhizosphere chamber which are aligned and stacked from top to bottom. The root chamber comprises sealed side walls and a bottom mesh fixed to the bottoms of the side walls, and the aperture of the bottom mesh is 25 micron to the maximum degree; the rhizosphere chamber is composed of a plurality of mesh-shaped inserting pieces which are stacked, each inserting piece comprises sealed supporting walls and a mesh piece supported by the supporting walls, and the aperture of the mesh pieces is 25 micron to the maximum degree; the non-rhizosphere chamber comprises sealed side walls and a bottom plate fixed to the bottoms of the side walls; a plurality of inserting grooves are formed outside the side walls of the root chamber, and plugs correspondingly inserted in the inserting grooves one to one are arranged outside the side walls of the non-rhizosphere chamber, so that the root chamber, the rhizosphere chamber and the non-rhizosphere chamber are integrated into a whole. By means of the immersed multicompartment submerged plant rhizosphere box, collection of sediment samples on a millimeter rhizosphere micro domain can be achieved conveniently and accurately, so that analysis and research of rhizosphere sediment samples can be conducted conveniently.
Description
Technical field
The present invention relates to immersion many compartments submerged plant rhizosphere case apparatus, particularly relate to a kind of rhizosphere case apparatus for the research of submerged plant rhizosphere micro-region.
Background technology
For submerged plant: rhizosphere is the center of plant, sediments and microorganism and environmental interaction thereof, being the region that between plant and Sediment environment, exchanges of mass and energy is the most violent, is also the most active place of microorganism.It has been the pith of domestic and international rhizosphere Pollution Chemistry research to the research of rhizosphere micro-region.
For the sampling method of Rhizosphere Soil, many employing root pad methods now.Now have the device of report to be three Room many interlayers root casees more, among plant be root room, the multiple interlayer in both sides is many interlayers rhizosphere room, and outermost is non-rhizosphere room.In order to utilize, root forms root pad on the vertical nylon wire on both sides, root room, many interlayers inserted sheet carries out a millimeter rank sampling.But this device is mainly used in the research of terrestrial plant rhizosphere micro-region.The most rhizosphere of terrestrial plant is flourishing, can assemble and form root pad on the nylon wire of both sides, root room, but submerged plant root system is undeveloped even degenerates, and is difficult to formation root pad with this device.
Summary of the invention
For prior art, the invention provides a kind of immersion many compartments submerged plant rhizosphere case, be applicable to the variation characteristic research of nutrient in submerged plant root system and sediments, pollutant and microorganism.Adopt device of the present invention to sample the sediments from root different distance easily and accurately, solve the technical barrier that in the research of submerged plant rhizosphere micro-region, sediments gathers.Use the present invention can simplify a millimeter method for rank rhizosphere micro-region sediments collection further, be conducive to the Regional Differentiation Characteristics studying submerged plant different piece, be conducive to the research of further meticulous root system-Sediment Interface.
In order to solve the problems of the technologies described above, a kind of immersion many compartments submerged plant rhizosphere case that the present invention proposes, comprise align from top to bottom stack root room, rhizosphere room and non-rhizosphere room; Described room comprises enclosed sidewall and is fixed on the spacer screen of sidewall bottom, and the aperture of spacer screen is 25 μm to the maximum; Described rhizosphere room is made up of several the netted inserted sheets mutually stacked, and each inserted sheet comprises enclosed supporting walls and supports mesh sheet by this supporting walls and forms, and the aperture of mesh sheet is 25 μm to the maximum; Described non-rhizosphere room comprises enclosed sidewall and is fixed on the base plate of sidewall bottom; The outside of root room sidewall is evenly equipped with multiple slot, and the outside of non-rhizosphere room sidewall is provided with the plug of multiple and described slot one_to_one corresponding grafting, thus by aggregates to root room, rhizosphere room and non-rhizosphere room structure.
Further, the shape of the floor projection of the described outdoor profile of room, rhizosphere room and non-rhizosphere is identical; Described room sidewall is tetragonal lucite frame; Described slot has four, is separately positioned on the central position of every face, root room sidewall; Described plug has four, is separately positioned on the central position of every face, non-rhizosphere room sidewall.
Compared with prior art, the invention has the beneficial effects as follows:
(1) inserted sheet of many interlayers rhizosphere room is adopted to design, can in-situ acquisition rhizosphere soil, well can reduce the disturbance of sampling, and accurately can study the sediments of distance root system different distance position.
(2) latching type is adopted root room, rhizosphere room and non-rhizosphere room to be fixed as an entirety, rhizosphere case entirety not only has detachability, can reuse, and the design of latch well can also fix the upper, middle, and lower part of rhizosphere case, the tightness of assurance device.
(3) the root pad of root system of plant is formed in bottom root room, efficiently solves the underdeveloped problem of submerged plant root system.
Accompanying drawing explanation
Fig. 1 is immersion many compartments submerged plant rhizosphere box structure schematic diagram of the present invention;
Fig. 2-1 is the vertical view of inserted sheet in the room of rhizosphere shown in Fig. 1;
Fig. 2-2 is A-A profiles of inserted sheet described in Fig. 2-1.
Embodiment
Be described in further detail technical solution of the present invention below in conjunction with the drawings and specific embodiments, described specific embodiment only explains the present invention, not in order to limit the present invention.
As shown in Figure 1, a kind of immersion many compartments of the present invention submerged plant rhizosphere case, for the sampling of submerged plant rhizodeposition thing, comprises and aligns the dismountable room 4 stacked, the rhizosphere room 3 of many interlayers and non-rhizosphere room 2 three part from top to bottom.Described room 4, rhizosphere room 3 are identical with the shape of the floor projection of non-rhizosphere room 2 outline.Described room 4 comprises enclosed sidewall and is fixed on the spacer screen of sidewall bottom, described room sidewall is tetragonal lucite frame, namely root room 4 is square lucite frames, bottom is stained with nylon wire, be nylon wire by the bottom design of root room 4 in the present invention, conveniently observe root pad formational situation.The aperture of spacer screen is 25 μm to the maximum.Described rhizosphere room 3 is made up of several the netted inserted sheets 8 mutually stacked, as shown in Fig. 2-1 and Fig. 2-2, each inserted sheet 8 comprises using machine instrument bezel as enclosed supporting walls 7 and supports mesh sheet (employing nylon6 chips) 6 by this supporting walls 7 and formed, nylon wire 6 is bonded at the side of lucite frame, the aperture of mesh sheet is 25 μm to the maximum, each inserted sheet 8 in the rhizosphere room 3 of many interlayers is each other for gapless is close to, and namely the supporting walls 7 of adjacent layer is close contact.As shown in Figure 1, described non-rhizosphere room 2 comprises enclosed sidewall and is fixed on the base plate of sidewall bottom.Rhizosphere room 3 and non-rhizosphere room 2 three part of root room 4, many interlayers are fixed by latch, latch comprises the plug 51 be fixed on non-rhizosphere room 3 and the slot 52 be fixed on root room 4, rhizosphere case of the present invention is detachable casing, namely multiple slot 52 is evenly equipped with in the outside of root room sidewall, the outside of non-rhizosphere room sidewall is provided with the plug 51 of multiple and described slot 52 one_to_one corresponding grafting, be that four directions is three-dimensional for rhizosphere case overall profile, then described slot 52 has four, is separately positioned on the central position of every face, root room sidewall; Described plug 51 has four, is separately positioned on the central position of every face, non-rhizosphere room sidewall, thus by aggregates to root room 4, rhizosphere room 3 and non-rhizosphere room 2 structure; Adopt the Plug Design being arranged on rhizosphere box main body surrounding that rhizosphere case upper, middle, and lower part can be made firmly to combine in the present invention, the tightness of assurance device.
Embodiment:
Fig. 1, Fig. 2-1 and Fig. 2-2 shows a kind of immersion many compartments submerged plant rhizosphere case apparatus, comprises rhizosphere case 1, non-rhizosphere room 2, the rhizosphere room 3 of many interlayers, root room 4.Rhizosphere case 1 is detachable casing, comprises the root room 4 on upper strata, the middle rhizosphere room 3 of many interlayers and the non-rhizosphere room 2 of lower floor from top to bottom.The rhizosphere room 3 of many interlayers is stacked by the inserted sheet 8 of multiple nylon material and forms, and nylon inserted sheet 8 comprises the mesh sheet 6 using lucite frame as carriage 7, nylon, and nylon wire is bonded on lucite frame; Rhizosphere room 3 and root room 4 three part of non-rhizosphere room 2, many interlayers are fixed by latch.Each one of latch surrounding, comprises the plug 51 be fixed on non-rhizosphere room 2 and the slot 52 be fixed on root room 4.This rhizosphere case 1 is processed into poly (methyl methacrylate) plate.
The bottom of root room 4 is stained with nylon wire, and the mesh diameter of nylon wire is 25 μm, root system both can have been stoped to pass, do not hindered again the migration of pollutant, nutrient and root exudates etc.Lucite frame is the quadrate support framework of interior sky, and border width is identical with the thickness of rhizosphere box main body poly (methyl methacrylate) plate, is 5mm.The thickness of frame was determined according to needing of studying of rhizosphere micro-region, and such as, during the sediments research of adjust the distance rhizosphere 1mm, 2mm, 3mm, 4mm etc., seal ring thickness is 1mm.During bonding, glue is coated on lucite frame, above the nylon wire being greater than instrument bezel area tightened is placed in, after bonding completely, cuts off the nylon wire that surrounding is unnecessary, be inserted sheet 8.The quantity of inserted sheet 8 is determined according to test requirements document, gaplessly each other when stacking fits tightly.Plug 51 is the lucite bar of thickness 5mm, wide 10mm, and its length can adjust according to the quantity of inserted sheet 8, is bonded at the middle position of four sides of non-rhizosphere room 2 from the bottom of non-rhizosphere room 2; Slot 52 is made up of two lucite bars, and the thickness of glass bar is 5mm, wide 15mm, and its length can according to the length adjustment of plug 51, and the spacing of two glass bars is 10mm, ensures the fixation of latch.
During the actual use of immersion many compartments submerged plant rhizosphere case of the present invention, first in the root room 4 on upper strata, put into the sediments (soil) that thickness is 20 ~ 30mm, sediments all crosses 2mm sieve, soak completely by suitable quantity of water, appropriate submerged plant is implanted by cuttage mode, stablize 15min, add water to top, root room 4, the mode that adds water is advisable not cause sediment disturbance.Put into water vat to cultivate, cultivated days is formed according to the root pad of different submerged plant root system to be determined, and can see root pad formational situation on the nylon wire of bottom.After the root pad of root system is formed, non-rhizosphere room 2 and nylon inserted sheet 8 fill up sediments respectively, and its weight is according to respective volume computing.Non-rhizosphere room 2 is soaked completely by suitable quantity of water, and the sediments in nylon inserted sheet 8 is uniformly distributed, and with overlaying successively after water-soaked on non-rhizosphere room 2, and fixes with plug 51.From water vat, take out kind of the root room 4 having plant, be placed on above many interlayers rhizosphere room 3, and be completely fixed with latch, ensure that it fits tightly.Finally, rhizosphere case 1 is put into water vat, tests.When sampling after off-test, take off root room, upper strata 4, take off each nylon inserted sheet 8 successively, carry out original position and be separated sampling.In the present invention, the inserted sheet quantity comprised in rhizosphere room 3 needs to arrange according to test, thus can be used for the sediment sample gathering distance rhizosphere different distance distance, realizes the sedimental original position of rhizosphere micro-region different mm level and is separated collection.
Although invention has been described by reference to the accompanying drawings above; but the present invention is not limited to above-mentioned embodiment; above-mentioned embodiment is only schematic; instead of it is restrictive; those of ordinary skill in the art is under enlightenment of the present invention; when not departing from present inventive concept, can also make a lot of distortion, these all belong within protection of the present invention.
Claims (4)
1. immersion many compartments submerged plant rhizosphere case, comprise align from top to bottom stack root room (4), rhizosphere room (3) and non-rhizosphere room (2), it is characterized in that:
Described room (4) comprises enclosed sidewall and is fixed on the spacer screen of sidewall bottom, and the aperture of spacer screen is 25 μm to the maximum;
Described rhizosphere room (3) is made up of several the netted inserted sheets mutually stacked, each inserted sheet comprises enclosed supporting walls (7) and supports mesh sheet (6) by this supporting walls (7) and forms, and the aperture of mesh sheet is 25 μm to the maximum;
Described non-rhizosphere room (2) comprises enclosed sidewall and is fixed on the base plate of sidewall bottom;
The outside of root room sidewall is evenly equipped with multiple slot (52), the outside of non-rhizosphere room sidewall is provided with the plug (51) of multiple and described slot (52) one_to_one corresponding grafting, thus by aggregates to root room (4), rhizosphere room (3) and non-rhizosphere room (2) structure.
2. immersion many compartments submerged plant rhizosphere case according to claim 1, is characterized in that: described room (4), rhizosphere room (3) are identical with the shape of the floor projection of non-rhizosphere room (2) outline.
3. immersion many compartments submerged plant rhizosphere case according to claim 2, is characterized in that: described room sidewall is tetragonal lucite frame.
4. immersion many compartments submerged plant rhizosphere case according to claim 3, is characterized in that: described slot (52) has four, is separately positioned on the central position of every face, root room sidewall; Described plug (51) has four, is separately positioned on the central position of every face, non-rhizosphere room sidewall.
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| CN201510617828.6A CN105165454A (en) | 2015-09-23 | 2015-09-23 | Immersed multicompartment submerged plant rhizosphere box |
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| CN201510617828.6A CN105165454A (en) | 2015-09-23 | 2015-09-23 | Immersed multicompartment submerged plant rhizosphere box |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108476795A (en) * | 2018-05-23 | 2018-09-04 | 江西省食品发酵研究所 | A kind of experiment Se-rich farm products planting unit convenient for classification plantation |
| CN110133200A (en) * | 2019-06-11 | 2019-08-16 | 东北大学 | It is a kind of for studying multi-functional case apparatus of plant rhizosphere microenvironment |
| CN110178581A (en) * | 2019-04-18 | 2019-08-30 | 北京师范大学 | Submerged plant root zone multielement distribution experiments device and method based on DGT technology |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108476795A (en) * | 2018-05-23 | 2018-09-04 | 江西省食品发酵研究所 | A kind of experiment Se-rich farm products planting unit convenient for classification plantation |
| CN110178581A (en) * | 2019-04-18 | 2019-08-30 | 北京师范大学 | Submerged plant root zone multielement distribution experiments device and method based on DGT technology |
| CN110133200A (en) * | 2019-06-11 | 2019-08-16 | 东北大学 | It is a kind of for studying multi-functional case apparatus of plant rhizosphere microenvironment |
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Application publication date: 20151223 |