CN104137761A - Testing apparatus and testing method for researching root system to deal with vertical obstacles - Google Patents
Testing apparatus and testing method for researching root system to deal with vertical obstacles Download PDFInfo
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- CN104137761A CN104137761A CN201410363552.9A CN201410363552A CN104137761A CN 104137761 A CN104137761 A CN 104137761A CN 201410363552 A CN201410363552 A CN 201410363552A CN 104137761 A CN104137761 A CN 104137761A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention relates to a testing apparatus for researching a root system to deal with vertical obstacles. The apparatus comprises a transparent cylindrical container for plant nursery stock growth, a plurality of transparent partition layers are arranged in the cylindrical container in the vertical direction to separate the interior of the cylindrical container into a plurality of layered regions for being filled with culture substrates, through holes for enabling every two vertically-adjacent layered regions to be communicated are formed in each partition layer, and testing holes are formed in the position, corresponding to each layered region, of the side wall of the cylindrical container. Meanwhile, the invention further provides a testing method for researching the root system to deal with the vertical obstacles. The testing device for researching the root system to deal with the vertical obstacles is simple in overall structure and low in manufacturing cost, material taking and manufacturing are convenient, the natural environment is simulated, through the device, how the root system starts the morphological physiological response strategy so as to deal with the growth adversity is researched, especially, the capability of the test plant root system for dealing with the solid obstacles in the vertical direction is researched, and the afforestation success rate under the difficult site conditions is improved.
Description
Technical field
The present invention relates to a kind of experimental rig and test method thereof of tackling vertical obstacle for studying root system, belong to the research field of afforestation technology under difficult land occupation condition.
Background technology
In recent years, China's forest coverage increases substantially, and some easier afforestations have completed afforestation task substantially.Yet along with socioeconomic fast development, the mankind are still very serious to the destruction in forest land, formed the difficulty of large area stone matter such as Abandoned Land of Mine, exposed massif, stone pit, Waste Landfill Site on the spot.How to improve these difficulties afforestation success rate on the spot, increasing green coverage is current forest workers's urgent problem.Take Abandoned Land of Mine as example, and the mankind increase day by day to the demand of mineral resources, to the exploitation of mineral resources, inevitably can produce destruction to vegetation and soil, and strip mining transformation, surface collapse, storing up of selecting and purchasing ore deposit discarded object have produced a large amount of Abandoned Land of Mines.According to Geological And Mineral Resources Departments In Their rough estimates, national mining area accumulative total damage to land area reaches 2,880,000 hm
2, every year with about 4.67 ten thousand hm
2speed increase.Not only brought Abandoned Land of Mine heavy metal pollution, water and soil loss, geological disaster hidden danger such as increase the weight of at the series of environmental problems, also cause afforestation survival rate, storage rate not high, this is mainly owing to usually meeting with the soil solid obstacles such as Shi Zhi in Root-development, the nutrient, moisture that causes Root Absorption be top growth demand contentedly, so that Stand Growth is slow, poor stability, stand quality is low, can not give play to higher benefit.
Yet, in long-term evolution process, some plant is strong especially to the perception of the soil adverse circumstances such as stone matter obstacle, and can start rapidly countermeasure, comprise morphological plasticity, physiological plasticity, mycorhiza plasticity etc., some plants can also be by regulating diameter, branch angle, interval and the steric configuration of radicula in root system to realize the countermeasures such as evading, detour to soil solid obstacle.Visible, how by research, fully excavate the potential ability of root system of plant reply soil solid obstacle, to improving the success rate of afforesting under difficult land occupation condition, realize difficulty on the spot quick recovering vegetation tool most important theories and practice significance.
Summary of the invention
In view of this, the object of this invention is to provide a kind of experimental rig and test method thereof of tackling vertical obstacle for studying root system, simple in structure, easily manufactured, simulating natural environment, the ability of researching plant root reply vertical direction solid obstacle, improves the success rate of afforesting under difficult land occupation condition.
The present invention adopts following scheme to realize: a kind of experimental rig of tackling vertical obstacle for studying root system, comprise the transparent cylinder container for plant seedling growth, in described cylindrical vessel, be vertically disposed with several lamina septum pellucidums cylindrical vessel interior separation is become to a plurality of for filling the delamination area of culture matrix, on each interlayer, be provided with the through hole that is communicated with upper and lower two adjacent delamination area, on the corresponding cylindrical vessel sidewall of each delamination area, offer instrument connection.
Further, described cylindrical vessel lateral wall and each instrument connection corresponding position are provided with the tinfoil hiding on instrument connection.
Further, in described cylindrical vessel, be provided with two lamina septum pellucidums, be respectively first interlayer of arranging from top to bottom and the second interlayer with by inner three delamination area of cylindrical vessel, be respectively first layer region, the second delamination area and the 3rd delamination area of arranging from top to bottom.
Further, on described the first interlayer, near cylindrical vessel madial wall place, be provided with a circular hole A, on described the second interlayer, be positioned at described circular hole A under be provided with the circular hole B that a diameter is identical with circular hole A, in the diametric(al) at described circular hole B place, be also arranged with the circular hole C that several diameters are identical with circular hole B.
Further, described circular hole B and circular hole C are equidistant arrangements in the diametric(al) at its place.
Simultaneously, the present invention also provides a kind of test method of tackling vertical obstacle for studying root system, adopt a kind of experimental rig of tackling vertical obstacle for studying root system as above, and carry out according to the following steps: (1) is prepared this experimental rig of predetermined quantity and is divided into some batches; (2) in each delamination area of each experimental rig, be placed with the culture matrix of identical nutrient, sowing or the colory plant seed of the predetermined quantity of planting or transplanting nursery stock in the delamination area of the top; (3) regularly utilize Type Water Potential Meter to pass through the flow of water that instrument connection on each experimental rig is measured different delamination area, and in flow of water delamination area on the low side, keep the skin wet or nutrient solution in time, guarantee the identical flow of water of delamination area tool of all experimental rigs; (4) regularly take out a collection of experimental rig, the root system in each delamination area is carried out to morphophysiology index determining, penetrate smoothly ability and the growth conditions of different through holes when observing root system tackle vertical obstacle; (5) record in each delamination area on each experimental rig of different batches, distribution and the growing state of each through hole root system.
Further, in described step (4), the method for morphophysiology index determining is to scan each root system image by digital scanning instrument, and applies image analysis software carries out quantitative analysis to the root system of each delamination area growth; And utilize gas chromatograph to carry out quantitative analysis to the physical signs of each delamination area growth root system.
Compared with prior art, the present invention has following beneficial effect: overall structure of the present invention is simple, cheap, draw materials with easy to make, simulating natural environment, when root system of plant is when meeting with the solid obstacles such as stone matter, slag, plastic products discarded object in ground vertical-growth process, how research root system starts morphophysiology response policy to tackle this growth adverse circumstance, especially the ability of test plant root system reply vertical direction solid obstacle, improves the success rate of afforesting under difficult land occupation condition.
Accompanying drawing explanation
Fig. 1 is embodiment of the present invention organigram;
Number in the figure explanation: 1-cylindrical vessel, 2-instrument connection, 3-the first interlayer, 4-the second interlayer, 5-first layer region, 6-the second delamination area, 7-the 3rd delamination area, 8-circular hole A, 9-circular hole B, 10-circular hole C.
Embodiment
For making object of the present invention, technical scheme and advantage clearer, below will, by specific embodiment and relevant drawings, the present invention be described in further detail.
As shown in Figure 1, a kind of experimental rig of tackling vertical obstacle for studying root system, comprise the transparent cylinder container 1 for plant seedling growth, in described cylindrical vessel 1, be vertically disposed with several lamina septum pellucidums cylindrical vessel interior separation is become to a plurality of for filling the delamination area of culture matrix, described cylindrical vessel 1 and interlayer adopt the fluid-tight hard plate material of printing opacity (as glass etc.) to make, to simulate the soil solid obstacle meeting with in root system vertical-growth process.On each interlayer, be provided with the through hole with root system free penetrating that is communicated with upper and lower two adjacent delamination area, on the corresponding cylindrical vessel sidewall of each delamination area, offer instrument connection 2, for observing root growth situation, acquisition test sample and keeping the skin wet or nutrient solution.
In the present embodiment, described cylindrical vessel 1 lateral wall and each instrument connection 2 corresponding positions are provided with the tinfoil hiding on instrument connection, and with the loss that prevents that water from dividing, each instrument connection 2 is positioned at the center in corresponding delamination area vertical direction.
In the present embodiment, in described cylindrical vessel, be provided with two lamina septum pellucidums, respectively first interlayer 3 of arranging from top to bottom and the second interlayer 4 with by inner three delamination area of cylindrical vessel, respectively first layer region 5, the second delamination area 6 and the 3rd delamination area 7 of arranging from top to bottom, the growing state of root system in each delamination area of observational study, especially after studying the impact that root system suffers vertical obstacle, the uniformity that root system distributes in each delamination area of the different soils degree of depth and with respect to the symmetry of the position of stem.
In the present embodiment, on described the first interlayer 3, near cylindrical vessel 1 madial wall place, be provided with a circular hole A(8), on described the second interlayer 4, be positioned at described circular hole A(8) under be provided with a diameter and circular hole A(8) identical circular hole B(9), described circular hole B(9) be also arranged with several diameters and circular hole B(9 in the diametric(al) at place) identical circular hole C(10); Described through hole on the first interlayer is circular hole A, and the described through hole on the second interlayer is circular hole B and circular hole C; Wherein circular hole A is for the form that forms between simulated soil solid obstacle or the space of different sizes, while straightly growing to first layer region 5 for researching plant root is vertical, when part root system meets with the obstruction of the first interlayer, whether this part root system can assemble growth to circular hole A by starting Morphology And Physiology countermeasure, thereby evades or the formed obstacle of the first interlayer that detoured; After root system enters the second delamination area 6 through circular hole A, we need to study the degree of depth of planting a colony that root system is hung down and straightly grown by circular hole B, also have, need to study root system and plant a colony range in the second delamination area, whether the direction of growth of assembling through the root system of circular hole A obtains adjustment, the Spatial Distribution Pattern that makes root system is systematicness, symmetry with respect to the position of stem, thereby is assembled equably and grown to the 3rd delamination area by the some circular hole C apart from circular hole B different distance; The comprehensive growing state of analyzing above-mentioned root system can disclose the morphophysiology strategy that root system of plant is tackled vertical obstacle.
In the present embodiment, described circular hole B(9) and circular hole C(10) in the diametric(al) at its place, be equidistant arrangement, for studying the interior root system of observation the second delamination area 6 through the uniformity of the second interlayer 4.
As shown in Figure 1, a kind of test method of tackling vertical obstacle for studying root system, comprise a kind of experimental rig of tackling vertical obstacle for studying root system as above, and carry out according to the following steps: (1) is prepared this experimental rig of predetermined quantity and is divided into some batches; (2) in each delamination area of each experimental rig, be placed with the culture matrix of identical nutrient, sowing sowing or the colory plant seed of the predetermined quantity of planting in the delamination area of the top (as pine and cypress seed etc.) or transplanting nursery stock (as pine and cypress nursery stock etc.); (3) regularly utilize Type Water Potential Meter to pass through the flow of water that instrument connection on each experimental rig is measured different delamination area, and in flow of water delamination area on the low side, keep the skin wet or nutrient solution in time, guarantee the identical flow of water of delamination area tool of all experimental rigs; Test and supplement complete employing tinfoil testing window is sealed, with the loss that prevents that water from dividing; (4) regularly take out a collection of experimental rig, the root system in each delamination area is carried out to morphophysiology index determining, penetrate smoothly ability and the growth conditions of different through holes when observing root system tackle vertical obstacle; (5) record in each delamination area on each experimental rig of different batches, distribution and the growing state of each through hole root system.
In the present embodiment, in described step (4), the method for morphophysiology index determining is to scan each root system image by digital scanning instrument, and applies image analysis software carries out quantitative analysis to the root system of each delamination area growth; And utilize gas chromatograph to carry out quantitative analysis to the physical signs (as endogenous hormones, organic acid etc.) of each delamination area growth root system.
Above-listed preferred embodiment; the object, technical solutions and advantages of the present invention are further described; institute is understood that; the foregoing is only preferred embodiment of the present invention; not in order to limit the present invention; within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (7)
1. an experimental rig of tackling vertical obstacle for studying root system, it is characterized in that: comprise the transparent cylinder container for plant seedling growth, in described cylindrical vessel, be vertically disposed with several lamina septum pellucidums cylindrical vessel interior separation is become to a plurality of for filling the delamination area of culture matrix, on each interlayer, be provided with the through hole that is communicated with upper and lower two adjacent delamination area, on the corresponding cylindrical vessel sidewall of each delamination area, offer instrument connection.
2. experimental rig of tackling vertical obstacle for studying root system according to claim 1, is characterized in that: described cylindrical vessel lateral wall and each instrument connection corresponding position are provided with the tinfoil hiding on instrument connection.
3. experimental rig of tackling vertical obstacle for studying root system according to claim 1 and 2, it is characterized in that: in described cylindrical vessel, be provided with two lamina septum pellucidums, be respectively first interlayer of arranging from top to bottom with the second interlayer cylindrical vessel interior separation is become to three delamination area, be respectively first layer region, the second delamination area and the 3rd delamination area of arranging from top to bottom.
4. experimental rig of tackling vertical obstacle for studying root system according to claim 3, it is characterized in that: on described the first interlayer, near cylindrical vessel madial wall place, be provided with a circular hole A, on described the second interlayer, be positioned at described circular hole A under be provided with the circular hole B that a diameter is identical with circular hole A, in the diametric(al) at described circular hole B place, be also arranged with the circular hole C that several diameters are identical with circular hole B.
5. experimental rig of tackling vertical obstacle for studying root system according to claim 4, is characterized in that: described circular hole B and circular hole C are equidistant arrangements in the diametric(al) at its place.
6. a test method of tackling vertical obstacle for studying root system, it is characterized in that: described in employing claim 1 ~ 5, any tackles the experimental rig of vertical obstacle for studying root system, and carries out according to the following steps: this experimental rig that (1) prepares predetermined quantity is also divided into some batches; (2) in each delamination area of each experimental rig, be placed with the culture matrix of identical nutrient, sowing or the colory plant seed of the predetermined quantity of planting or transplanting nursery stock in the delamination area of the top; (3) regularly utilize Type Water Potential Meter to pass through the flow of water that instrument connection on each experimental rig is measured different delamination area, and in flow of water delamination area on the low side, keep the skin wet or nutrient solution in time, guarantee the identical flow of water of delamination area tool of all experimental rigs; (4) regularly take out a collection of experimental rig, the root system in each delamination area is carried out to morphophysiology index determining, penetrate smoothly ability and the growth conditions of different through holes when observing root system tackle vertical obstacle; (5) record in each delamination area on each experimental rig of different batches, distribution and the growing state of each through hole root system.
7. the test method of tackling vertical obstacle for studying root system according to claim 6, it is characterized in that: in described step (4), the method for morphophysiology index determining is to scan each root system image by digital scanning instrument, applies image analysis software carries out quantitative analysis to the root system of each delamination area growth; And utilize gas chromatograph to carry out quantitative analysis to the physical signs of each delamination area growth root system.
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| CN104137761B CN104137761B (en) | 2016-02-17 |
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| CN105403569A (en) * | 2015-12-21 | 2016-03-16 | 福建农林大学 | Device and method for evaluating the vertical growth avoidance of plant roots from pollutants |
| CN106769166A (en) * | 2016-12-06 | 2017-05-31 | 河海大学 | A kind of experimental provision for the fine Stratified Sampling of green plant rhizosphere soil |
| CN107249306A (en) * | 2015-02-10 | 2017-10-13 | 新成长系统有限公司 | The facility cultivated for water planting |
| CN108605570A (en) * | 2017-10-31 | 2018-10-02 | 鞠林 | A kind of root system net sheet type forming method |
| CN108713493A (en) * | 2018-07-30 | 2018-10-30 | 安徽农业大学 | For analyzing the water planting potting bucket for heating and being influenced on wheat root distribution |
| CN108925281A (en) * | 2018-07-30 | 2018-12-04 | 安徽农业大学 | For analyzing the potting bucket for heating and influencing on wheat root distribution |
| CN114402846A (en) * | 2022-01-26 | 2022-04-29 | 河北省林业和草原科学研究院 | Root system observation box |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108605570A (en) * | 2017-10-31 | 2018-10-02 | 鞠林 | A kind of root system net sheet type forming method |
| CN108713493A (en) * | 2018-07-30 | 2018-10-30 | 安徽农业大学 | For analyzing the water planting potting bucket for heating and being influenced on wheat root distribution |
| CN108925281A (en) * | 2018-07-30 | 2018-12-04 | 安徽农业大学 | For analyzing the potting bucket for heating and influencing on wheat root distribution |
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| CN104137761B (en) | 2016-02-17 |
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