CN202799764U - Testing device for researching root system phenotypic plasticity - Google Patents
Testing device for researching root system phenotypic plasticity Download PDFInfo
- Publication number
- CN202799764U CN202799764U CN 201220460345 CN201220460345U CN202799764U CN 202799764 U CN202799764 U CN 202799764U CN 201220460345 CN201220460345 CN 201220460345 CN 201220460345 U CN201220460345 U CN 201220460345U CN 202799764 U CN202799764 U CN 202799764U
- Authority
- CN
- China
- Prior art keywords
- root system
- square tube
- testing device
- square cylinder
- zone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The utility model relates to a testing device for researching root system phenotypic plasticity. The testing device comprises a square cylinder for seeding plant seeds. The testing device is characterized in that a vertical separation layer is arranged on the square cylinder along the central vertical direction, so that two feeding regions used for placing nutrient patches with different nutrients are separated; a bottom plate is arranged at the bottom of the square cylinder; the square cylinder and the vertical separation layer are made of nonopaque and water-proof rigid plates, so that the root system interpenetration and nutrients flowing in the feeding region can be separated; a testing window is arranged on the side wall of the square cylinder; and a seeding slot is formed at the middle upper side of the vertical separation layer. The testing device can research the ability that after the soil nutrients are used up with time, the local root system of the plant senses the environment change and starts adaptive strategy, in particular the ability of root system phenotypic plasticity of the tested plants under a state most close to nature. The device is simple in integral structure, low in cost and convenient to manufacture, and materials can be elected conveniently.
Description
Technical field
The utility model relates to the research field of plant adapted soil nutrient special heterogeneity, relates generally to the research of root system of plant phenotypic plasticity aspect.
Background technology
Content of available nutrient in soils is one of important attribute of the ecosystem, and soil nutrient spatial heterogeneity is prevalent in the natural ecosystems, and the space scale of Content of available nutrient in soils changes greatly, can reach by the scope of plant individual root system perception.Plant in order to obtain to greatest extent soil resource, produces various Plasticity in responses to the special heterogeneity of nutrient in the long-term evolution process, comprise morphological plasticity, physiological plasticity, mycorhiza plasticity etc.The root system of many plant speciess is a large amount of hyperplasia in the patch that nutrient enriches, and the hyperplasia degree interspecific difference is larger, and is subjected to patch attribute (Patch size, nutrient density), nutritive element kind and nutrient totally to supply the impact of situation.Plant is also realized the efficient utilization of patch nutrient by adjusting rich diameter, branch angle, interval and steric configuration of supporting radicula in the patch.
Summary of the invention
The utility model provides a kind of experimental rig for research root system phenotypic plasticity, utilize this experimental rig can be under the state near nature, research plant Local Root ties up to soil nutrient in time behind the approach exhaustion, its root system perception environmental change also starts ability, the especially ability of test plant root system phenotypic plasticity of adaptive strategy.
The technical solution of the utility model is: a kind of experimental rig for research root system phenotypic plasticity, comprise the square tube for the sowing plant seed, it is characterized in that: described square tube is provided with 1 vertical interlayer along the central vertical direction, to be separated out 2 keeping zones that are used for the nutrient patch of placement Different Nutrients, the bottom of described square tube is provided with base plate, described square tube and vertical interlayer all adopt the fluid-tight hard plate material of printing opacity to make, to intercept different the flowing of the interior root system IPN in zone and nutrient of supporting, offer testing window on the described square tube sidewall, the middle part upside of described vertical interlayer is provided with planting tank.
In one embodiment, described testing window is positioned at each center of supporting the zone, hides tinfoil on the described testing window, with the loss that prevents that water from dividing.
In one embodiment, described hard plate material is glass.
Another technical scheme of the present utility model is: a kind of test method for research root system phenotypic plasticity, comprise above-mentioned experimental rig, and it is characterized in that, carry out according to the following steps:
(1) get the colory plant seed of some, plant seed be seeded in the planting tank of above-mentioned experimental rig, and in the keeping zone of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;
(2) the above-mentioned experimental rig that is placed with plant seed and nutrient patch is divided into some batches;
(3) regularly measure the different flows of water of supporting the zone of each experimental rig by testing window after, adopt tinfoil that testing window is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get each and support the interior root system in zone to carry out the mensuration of morphological indexes.
(5) record test data: each supports distribution and the growing state of root system in the zone different time sections.
In above-mentioned steps (3), utilize Type Water Potential Meter to measure each flow of water of supporting the zone by described square tube testing window after, in time keep the skin wet or nutrient solution to supporting in the zone, until the different identical flow of water of regional tools of supporting.
In above-mentioned steps (4), get the root system that each supports the zone, scan each root system image by the digital scanning instrument, applies image analysis software supports the root system of region growing to carry out quantitative analysis to each.
The utility model has the advantage of: experimental rig of the present utility model can be under the state near nature, research plant Local Root ties up to soil nutrient in time behind the approach exhaustion, its root system perception environmental change also starts ability, the especially ability of test plant root system phenotypic plasticity of adaptive strategy.Its overall structure is simple, and is cheap, draws materials with easy to make.
Description of drawings
Fig. 1 is the structural representation of experimental rig of the present utility model.
Among the figure: 1-vertically interlayer, 2-keeping zone, 3-testing window, 4-square tube sidewall, 5-square tube base plate, 6-planting tank.
Embodiment
By describing technology contents of the present utility model, structural feature in detail, realized purpose and effect, below in conjunction with embodiment and cooperate that accompanying drawing is detailed to give explanation.
The structural representation of experimental rig of the present utility model as shown in Figure 1, the sidepiece of this figure adopt the mode of perspective to represent, with abundant displaying layer of the present utility model shelf structure, the other side is the diagram of profile.
Such as Fig. 1, a kind of experimental rig for research root system phenotypic plasticity, comprise square tube and the vertical interlayer that is nested with in the middle of square tube for the sowing plant seed, described square tube is provided with a vertical interlayer 1 along the central vertical direction, to be separated out the two keeping zones 2 for the nutrient patch of placing Different Nutrients, described vertical interlayer 1 center is provided with a planting tank 6, the seed that is used for the sowing test plant, be laid with one on described square tube two walls parallel with vertical interlayer and lead to the testing window 3 that each supports the zone, described square tube sidewall 4, square tube base plate 5 and vertical interlayer 1 are the fluid-tight hard plate material of printing opacity (such as glass), to intercept different the flowing of the interior root system IPN in zone and nutrient of supporting.
In the present embodiment, described vertical interlayer is arranged on the Central Symmetry face position of square tube, and described square tube and vertical interlayer consist of 2 and wait shape to support the zone.
In the present embodiment, described testing window (measure the flow of water, guarantee that the flow of water in 2 keeping zones is identical) is arranged on the center that each supports the zone, hides tinfoil on the described testing window, with the loss that prevents that water from dividing.
A kind of test method for research root system phenotypic plasticity comprises above-mentioned experimental rig, carries out according to the following steps:
(1) get the colory plant seed of some, plant seed be seeded in the planting tank of above-mentioned experimental rig, and in the keeping zone of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;
(2) the above-mentioned experimental rig that is placed with plant seed and nutrient patch is divided into some batches;
(3) regularly measure the different flows of water of supporting the zone of each experimental rig by testing window after, adopt tinfoil that testing window is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get each and support the interior root system in zone to carry out the mensuration of morphological indexes.
(5) record test data: each supports distribution and the growing state of root system in the zone different time sections.
Above-mentioned steps (3) is supported the identical flow of water of regional tool for guaranteeing each, after utilizing Type Water Potential Meter to measure each flow of water of supporting the zone by described square tube testing window, in time keep the skin wet or nutrient solution to supporting in the zone, until the different identical flow of water of regional tool of supporting.
The root system that above-mentioned steps (4) is supported the zone for getting each scans each root system image by the digital scanning instrument, and applies image analysis software supports the root system of region growing to carry out quantitative analysis to each.
Embodiment is as follows more specifically:
Study the phenotypic plasticity of China fir root system in the heterogeneous phosphorus supply environment: consisting of square tube sidewall, base plate and interlayer is the thick glass material of 2mm, require the square tube seam closely water-tight, Chinese fir seeds is seeded in the middle of the square tube vertically in the planting tank of interlayer (non-treatment region), until seed sprouting take root rear root system can free growth to treatment region.When carrying out the heterogeneous phosphorus processing of soil, support in the zone (treatment region) at each respectively and apply granular long-acting slow-release phosphorus source patch.Sand culture matrix is vanadine (Al
2O
3Cross the 28-48 order) ratio and quartz sand mixing with 1.5%, need alumina is carried out dephosphorization treatment before the dress basin, first with clear water flushing alumina to water without muddiness, soak 24h with 0.01mol/L HCl again, with clear water the alumina surface acid is rinsed well more afterwards, soak 24h with 0.01mol/L HCl, so reprocessing is 10 times, and is to guarantee the abundant wash-out of the phosphorus in the alumina, air-dry stand-by.Quartz sand is also used clear water and is cleaned to guarantee the abundant wash-out of its phosphorus.The used matrix of the phosphorus source patch KH of variable concentrations
2PO
4Soak to mix sand, utilize effectively Phosphate Sorption and can slowly evenly discharge the characteristic of available phosphorus of vanadine, control cultivation medium to the supply of available phosphorus, the KH that applies
2PO
4Use first dissolved in distilled water, alumina mixes immersion 24h with solution, and the dried sand after the adding quantitatively mixes again.Duration of test carries out dephosphorization element outer normal nutrient solution and moisture supply, after utilizing Type Water Potential Meter to measure each flow of water of supporting the zone by described square tube testing window, in time keep the skin wet or nutrient solution to supporting in the zone, until the different identical flows of water of regional tool of supporting, the flow of water is measured and is utilized after complete tinfoil to hide testing window, with the loss that prevents that water from dividing.After the test processing time finishes, divide patch to process in device, get the root system that each supports the zone, scan each root system image by the digital scanning instrument, applies image analysis software carries out quantitative analysis to each layer root system.
The above only is preferred embodiment of the present utility model; be not so limit claim of the present utility model; every equivalent structure or equivalent flow process conversion that utilizes the utility model specification and accompanying drawing content to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present utility model.
Claims (3)
1. experimental rig that is used for research root system phenotypic plasticity, comprise the square tube for the sowing plant seed, it is characterized in that: described square tube is provided with 1 vertical interlayer along the central vertical direction, to be separated out 2 keeping zones that are used for the nutrient patch of placement Different Nutrients, the bottom of described square tube is provided with base plate, described square tube and vertical interlayer all adopt the fluid-tight hard plate material of printing opacity to make, to intercept different the flowing of the interior root system IPN in zone and nutrient of supporting, offer testing window on the described square tube sidewall, the middle part upside of described vertical interlayer is provided with planting tank.
2. experimental rig according to claim 1 is characterized in that: described testing window is positioned at each center of supporting the zone, hides tinfoil on the described testing window, with the loss that prevents that water from dividing.
3. experimental rig according to claim 1, it is characterized in that: described hard plate material is glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220460345 CN202799764U (en) | 2012-09-11 | 2012-09-11 | Testing device for researching root system phenotypic plasticity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220460345 CN202799764U (en) | 2012-09-11 | 2012-09-11 | Testing device for researching root system phenotypic plasticity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202799764U true CN202799764U (en) | 2013-03-20 |
Family
ID=47858724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220460345 Expired - Fee Related CN202799764U (en) | 2012-09-11 | 2012-09-11 | Testing device for researching root system phenotypic plasticity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202799764U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860227A (en) * | 2012-09-11 | 2013-01-09 | 福建农林大学 | Test device and method used for studying root phenotypic plasticity |
-
2012
- 2012-09-11 CN CN 201220460345 patent/CN202799764U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860227A (en) * | 2012-09-11 | 2013-01-09 | 福建农林大学 | Test device and method used for studying root phenotypic plasticity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102860227A (en) | Test device and method used for studying root phenotypic plasticity | |
| CN102499045B (en) | Test device and method for studying foraging behavior of root system in horizontal direction | |
| CN102369877B (en) | Test device and method for researching on nutrient foraging behavior of root system in vertical direction | |
| CN104380959B (en) | Rapid cultivation method for loess plateau moss crusts | |
| CN104137761B (en) | A kind of experimental rig and test method thereof tackling vertical obstacle for studying root system | |
| Zhang et al. | Changes in physical properties of a loess soil in China following two long-term fertilization regimes | |
| CN208523337U (en) | A kind of Root Growth of Rice configuration observation device | |
| CN104062417B (en) | The structure of micro-territory, a kind of field test model and sample collection method | |
| CN205454803U (en) | Observe device that rice root system is grown and is distributed | |
| CN103960038B (en) | Test device and method for researching root competition strategy | |
| CN105766118B (en) | A kind of shoal soil body reconstructing method for Rice Cropping | |
| CN203872628U (en) | Testing device for researching on root system competitive strategy | |
| CN105432348B (en) | Rhizosphere simulates culture apparatus and its application | |
| CN105841995B (en) | The sampler and sampling method of a kind of plant rhizosphere and non-rhizosphere soils | |
| CN202799764U (en) | Testing device for researching root system phenotypic plasticity | |
| CN205103235U (en) | Survey test device is observed to soil body plant roots angle | |
| CN204466515U (en) | The device of changes of root morphology is made mutually between a kind of non-destructive observation of plant kind | |
| CN104126493B (en) | A kind of experimental rig and test method thereof tackling horizontal obstacle for studying root system | |
| CN105340597B (en) | Using the identification method for the crops seedling stage drought resistance for oozing rehydration on bottom | |
| CN202310748U (en) | Test device for researching foraging behavior of root system in vertical direction | |
| CN202310791U (en) | Test device used for researching horizontal-direction foraging behavior of root system | |
| CN203745032U (en) | Device for measuring plant rhizosphere trace gas during in-situ observation close natural wetland water level fluctuation process | |
| CN109856340A (en) | A method of research sediment siltation grows Spartina alterniflora and Root Distribution influences | |
| CN204929804U (en) | Be applicable to the From The Rhizosphere of Rice soil gathering unit in cadmium pollution soil analog study | |
| CN205727285U (en) | A kind of Rhizophora apiculata Blume offshore device for raising seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130320 Termination date: 20150911 |
|
| EXPY | Termination of patent right or utility model |