CN103960038B - Test device and method for researching root competition strategy - Google Patents
Test device and method for researching root competition strategy Download PDFInfo
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- CN103960038B CN103960038B CN201410232847.2A CN201410232847A CN103960038B CN 103960038 B CN103960038 B CN 103960038B CN 201410232847 A CN201410232847 A CN 201410232847A CN 103960038 B CN103960038 B CN 103960038B
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Abstract
The invention provides a test device and method for researching a root competition strategy. The test device comprises a center cylinder for simulating the root competition strategy of a plant and a concentric cylinder which is concentrically sleeved at the outer side of the center cylinder, wherein a plurality of vertical interlayers are evenly distributed in the area between the center cylinder and the concentric cylinder at the outer layer along the radial direction, so as to form a plurality of sector areas for containing nutrients; and bottom plates are arranged at the bottoms of the center cylinder and the concentric cylinder. The test device disclosed by the invention can research the capacity of a plant of sensing environment change and starting competitive strategy, especially tests the capacity of the plant root systems of expanding and occupying space along with increase of competition intensity to water and fertilizer resources in soil under the simulated nature state, and the test device is simple in overall structure, low in construction cost and convenient in material preparation and fabrication.
Description
Technical field
The present invention relates to the research field of root system of plant competitive behavior, relates generally to the heterogeneous distributional environment of soil resource speckle
In, the research in terms of different plants or kindred plant root competition behavior is specifically a kind of to be used to study root competition plan
Assay device slightly and method.
Background technology
Because plant equally moves freely in natural environment without image of Buddha animal, constantly meet with whole life cycle
By in planting total environmental resource is competed with inter-species.Therefore, competition is present in all phytobiocoenoses, is to determine vegetal inspired
One of state, life cycle and structure of plant community and dynamic major impetus.With aerial partss only to light competing phase ratio, underground part
The competition for dividing is related to the soil resources such as moisture, mineral, and the competition between plant is taken place mostly between the root system of underground.Plant pair
The competitiveness of soil moisture and nutrient depend on soil space occupied by its root system, the form of root system and physiological plasticity,
The Spatial Distribution Pattern of root system in soil etc..However, because root growth is in underground, growth rhythm change is complicated, and soil
Height heterogeneity is presented on the distribution space-time of moisture and nutrients resource, root system observation and sampling rate in Traditional Scientific research process
It is more difficult, it is difficult to study the competitive strategy for disclosing root system of plant in soil effectively.
The content of the invention
The present invention is improved the problems referred to above, i.e., the technical problem to be solved in the present invention is Traditional Scientific research process
Middle root system is observed and sampled relatively difficult, it is difficult to study the competitive strategy for disclosing root system of plant in soil effectively.
Technical program of the present invention lies in：A kind of assay device for studying root competition strategy, it is characterised in that bag
Include the center drum and the concentric drums that are nested with one heart on the outside of center drum for simulating plant root competition region, it is described in
Region between heart cylinder and outer layer concentric drums is radially uniformly laid with several vertical interlayers, some to be separated out
The bottom of the individual sector region for placing nutrient, the center drum and concentric drums is provided with base plate.
Further, the center drum, concentric drums and vertical interlayer adopt the fluid-tight hard material system of printing opacity
Into to intercept the flowing of root system IPN and nutrient in different keeping regions.
Further, test is offered on the lateral wall of several sector regions that the concentric drums are separated
Window, the inwall upside of several sector regions that the concentric drums are separated is provided with planting tank.
The present invention another technical scheme be：A kind of test method for studying root competition strategy, including such as power
Profit requires a kind of assay device for studying root competition strategy described in 3, it is characterised in that carry out according to the following steps：
（1）The colory plant seed of certain amount is taken, plant seed is seeded in into the planting tank of above-mentioned assay device
It is interior, and it is placed with the culture matrix of Different Nutrients respectively in the center drum and sector region of each assay device, wherein often
Individual sector region is supported identical；
（2）The above-mentioned assay device for being placed with plant seed and Nutrient patches is divided into into some batches；
（3）After the flow of water in the different keeping regions of each assay device is periodically determined by testing window, will be tested using tinfoil
Window is sealed, to prevent the loss of water point；
（4）Periodically take out batch of assay device to be observed：Taking each supports the root system in region to carry out form and life
The measure of index of science, emphasis observes the competition plan of different plants or kindred plant root system in each assay device center drum
Slightly；
（5）Record test data：Different time sections respectively support the distribution of root system and growing state in region.
Further, the step（3）In, each is measured by the square tube testing window using Type Water Potential Meter and supports area
After the flow of water in domain, keep the skin wet or nutritional solution into keeping region in time, until different support regions to have the identical flow of water.
Further, the step（4）In, each root system for supporting region is taken, scan each by digital scanner
It is image, applies image analysis software carries out quantitative analyses to the root system of each keeping region growing；Using high performance liquid chromatograph
The physiologic index such as organic acid are secreted to the root system in Competition for Nutrients region it is measured analysis Deng instrument.
Compared with prior art, the invention has the advantages that：The assay device of the present invention can be in simulation nature
In the state of, with continuing to increase to soil liquid manure resource contention intensity, its root system perceives environmental change simultaneously to researching plant root
Start the ability of competitive strategy, especially test plant root system expands the ability for capturing space.Its overall structure is simple, low cost
It is honest and clean, draw materials with it is easy to make.
Description of the drawings
Fig. 1 is the structural representation of the assay device of the present invention.
In figure：1-center drum, 2-concentric drums, 3-vertical interlayer, 4-sector region, 5-base plate, 6-sowing
Groove, 7-testing window.
Specific embodiment
With reference to the accompanying drawings and detailed description the present invention will be further described in detail.
A kind of embodiment of the present invention is illustrated in figure 1, including the center drum for simulating plant root competition region
The concentric drums 2 in the outside of center drum 1 are nested with one heart, and the region between center drum 1 and outer layer concentric drums 2 is radially
Direction is uniformly laid with several vertical interlayers 3, to be separated out several for placing identical nutrient（Or different in moisture, below
As a example by to place Different Nutrients）Nutrient patches sector region 4, the bottom of center drum 1 and concentric drums 2 is provided with bottom
Plate 5, the center drum 1, concentric drums 2 and is vertically made, the present embodiment every 3 layers using the fluid-tight hard material of printing opacity
Middle hard material is glass, to intercept the flowing of root system IPN and nutrient in different keeping regions.
In the present embodiment, to have four, vertical interlayer is by region between adjacent two cylinder etc. for several vertical interlayers
It is divided into four sector regions.Testing window 7 is offered on the lateral wall of several sector regions that concentric drums are separated,
The inwall upside of several sector regions that concentric drums are separated is provided with planting tank 6, i.e., in the upper end of center drum
Face is provided with the planting tank 6 of circular groove shape.Testing window 7 is located at the center that each sector supports region, covers on testing window 7
Tinfoil, to prevent the loss of water point.
The present invention another technical scheme be：A kind of test method for studying root competition strategy, including such as power
Profit requires a kind of assay device for studying root competition strategy described in 3, carries out according to the following steps：
（1）The colory plant seed of certain amount is taken, plant seed is seeded in into the planting tank of above-mentioned assay device
It is interior, and it is placed with the culture matrix of Different Nutrients respectively in the center drum and sector region of each assay device, wherein often
Individual sector region is supported identical；
（2）The above-mentioned assay device for being placed with plant seed and Nutrient patches is divided into into some batches；
（3）After the flow of water in the different keeping regions of each assay device is periodically determined by testing window, will be tested using tinfoil
Window is sealed, to prevent the loss of water point；
（4）Periodically take out batch of assay device to be observed：Taking each supports the root system in region to carry out form and life
The measure of index of science, emphasis observes the competition plan of different plants or kindred plant root system in each assay device center drum
Slightly；
（5）Record test data：Different time sections respectively support the distribution of root system and growing state in region.
In above-mentioned steps（3）In, each water for supporting region is measured by the square tube testing window using Type Water Potential Meter
After gesture, keep the skin wet or nutritional solution into keeping region in time, until different support regions to have the identical flow of water.
In above-mentioned steps（4）In, each root system for supporting region is taken, each root system image is scanned by digital scanner,
Applies image analysis software carries out quantitative analyses to the root system of each keeping region growing；Using instruments pair such as high performance liquid chromatographs
The physiologic index such as the root system secretion organic acid in Competition for Nutrients region are measured analysis.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (3)
1. a kind of a kind of test method for studying root competition strategy, including test for studying root competition strategy is filled
Put, it is characterised in that：
A kind of assay device for studying root competition strategy is included for the center in simulating plant root competition region
Cylinder and the concentric drums being nested with one heart on the outside of center drum, the region edge between the center drum and outer layer concentric drums
Radial direction is uniformly laid with several vertical interlayers, to be separated out several for placing the sector region of nutrient, it is described in
The bottom of heart cylinder and concentric drums is provided with base plate, and the center drum, concentric drums and vertical interlayer adopt printing opacity not
Permeable hard material is made, and to intercept the flowing of root system IPN and nutrient in different keeping regions, the concentric drums are divided
Be separated out on the lateral wall of several sector regions for coming and offer testing window, the concentric drums separated several
The inwall upside of sector region is provided with planting tank, carries out according to the following steps：
（1）The colory plant seed of certain amount is taken, plant seed is seeded in the planting tank of above-mentioned assay device, and
It is placed with the culture matrix of Different Nutrients respectively in the center drum and sector region of each assay device, wherein each is fan-shaped
Support identical in region；
（2）The above-mentioned assay device for being placed with plant seed and Nutrient patches is divided into into some batches；
（3）After the flow of water in the different keeping regions of each assay device is periodically determined by testing window, testing window is sealed using tinfoil
Firmly, the loss to prevent water from dividing；
（4）Periodically take out batch of assay device to be observed：Taking each supports the root system in region to carry out form and physiology
The measure of index, emphasis observes the competitive strategy of different plants or kindred plant root system in each assay device center drum；
（5）Record test data：Different time sections respectively support the distribution of root system and growing state in region.
2. a kind of test method for studying root competition strategy according to claim 1, the step（3）In, profit
Measured after the flow of water that each supports region by testing window with Type Water Potential Meter, kept the skin wet or nutrition into keeping region in time
Liquid, until different support region to have the identical flow of water.
3. a kind of test method for studying root competition strategy according to claim 1, the step（4）In, take
Each supports the root system in region, and by digital scanner each root system image is scanned, and applies image analysis software is to each keeping area
The root system of domain growth carries out quantitative analyses；Organic acid physiology is secreted to the root system in Competition for Nutrients region using high performance liquid chromatograph
Learn index and be measured analysis.
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| CN105432351A (en) * | 2015-12-21 | 2016-03-30 | 福建农林大学 | Device and method for testing mutual influences of plants and soil animals in polluted environment |
| CN106718255A (en) * | 2017-03-10 | 2017-05-31 | 广州花云网络科技有限公司 | Plant root growth test container and its method of testing |
| CN107646411A (en) * | 2017-10-17 | 2018-02-02 | 沈阳大学 | A kind of plant root growth experimental provision |
| CN109315177B (en) * | 2018-09-19 | 2024-01-19 | 湖南人文科技学院 | Device and method for comparing competition of crop root system to space and resources |
| CN109580882A (en) * | 2018-12-06 | 2019-04-05 | 农法自然（上海）农业科技有限公司 | Device and its measuring method of the different fertilizer to same strain plant root effects |
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| CN101502219A (en) * | 2009-03-06 | 2009-08-12 | 中国科学院东北地理与农业生态研究所 | Split crop root system cultivation device and cultivation method thereof |
| CN201995377U (en) * | 2010-11-25 | 2011-10-05 | 中国热带农业科学院南亚热带作物研究所 | Simple crop root division culture box |
| CN102124933A (en) * | 2010-12-02 | 2011-07-20 | 北京市农林科学院 | Method for researching partial root drying irrigation technology of woody fruit trees |
| CN102160504A (en) * | 2011-01-13 | 2011-08-24 | 南京林业大学 | Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage |
| CN102499045B (en) * | 2011-11-05 | 2013-05-15 | 福建农林大学 | Test device and method for studying foraging behavior of root system in horizontal direction |
| CN102369877B (en) * | 2011-11-05 | 2013-04-17 | 福建农林大学 | Test device and method for researching on nutrient foraging behavior of root system in vertical direction |
| CN202396262U (en) * | 2012-01-11 | 2012-08-29 | 河南科技大学 | Root division soilless cultivation and permanent planting box for experiments |
| CN202485959U (en) * | 2012-03-05 | 2012-10-10 | 华中农业大学 | Root box testing device for plant cultivation |
| CN102860227A (en) * | 2012-09-11 | 2013-01-09 | 福建农林大学 | Test device and method used for studying root phenotypic plasticity |
| CN103238398A (en) * | 2013-05-30 | 2013-08-14 | 安徽科技学院 | Plant seed germination box for root division test |
| CN203467298U (en) * | 2013-09-29 | 2014-03-12 | 吉林省农业科学院 | Testing device for studying plant root system growing state through simulation of soil local deep scarification |
| CN203872628U (en) * | 2014-05-29 | 2014-10-15 | 福建农林大学 | Testing device for researching on root system competitive strategy |
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