CN102369877B - Test device and method for researching on nutrient foraging behavior of root system in vertical direction - Google Patents
Test device and method for researching on nutrient foraging behavior of root system in vertical direction Download PDFInfo
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- CN102369877B CN102369877B CN2011103456474A CN201110345647A CN102369877B CN 102369877 B CN102369877 B CN 102369877B CN 2011103456474 A CN2011103456474 A CN 2011103456474A CN 201110345647 A CN201110345647 A CN 201110345647A CN 102369877 B CN102369877 B CN 102369877B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention relates to a test device and method for researching on the nutrient foraging behavior of a root system in a vertical direction. The device comprises a central cylinder which is used for transplanting a plant seedling and an outer cylinder which is concentrically sheathed on the external side of the central cylinder. The device is characterized in that a plurality of horizontal isolating layers are sequentially arranged in an area between the central cylinder and the outer cylinder from the bottom to the top along an axis direction and a plurality of vertical isolating layers are arranged in the area between the central cylinder and the outer cylinder along a radial direction so as to form a plurality of sector areas used for placing nutrient patches with different nutrients, discharge holes which are communicated with the sector areas are correspondingly arranged on the circumferential wall of the outer cylinder, the walls of the two concentric cylinders, the horizontal isolating layers and the vertical isolating layers are fiber glass nets, and agar is coated on the fiber glass nets to ensure that the root system of the plant can successfully penetrate through the fiber glass nets and the nutrients in the different sector areas are prevented from flowing at the same time. By using the test device, the ability of the root system in sensing and developing new nutrient patches from near to far in a state closest to a natural state after rhizosphere nutrients are used up with time can be researched and especially the ability of the root system of the plant in foraging the nutrients can be tested.
Description
Technical field
The present invention relates to the research field that plant looks for the behavior of supporting, relate generally to the research that the root system of plant vertical direction is looked for the behavior of supporting, specifically a kind of experimental rig and method of looking for the behavior of supporting for research root system vertical direction.
Background technology
Content of available nutrient in soils is one of important attribute of the ecosystem, and soil nutrient spatial heterogeneity is prevalent in the natural ecosystems, and the space scale of Content of available nutrient in soils changes greatly, can reach by the scope of plant individual root system perception.Plant in order to obtain to greatest extent soil resource, produces various Plasticity in responses to the special heterogeneity of nutrient in the long-term evolution process, comprise morphological plasticity, physiological plasticity, mycorhiza plasticity etc.The root system of many plant speciess is a large amount of hyperplasia in the patch that nutrient enriches, and the hyperplasia degree interspecific difference is larger, and is subjected to patch attribute (Patch size, nutrient density), nutritive element kind and nutrient totally to supply the impact of situation.Plant is also realized the efficient utilization of patch nutrient by adjusting rich diameter, branch angle, interval and steric configuration of supporting radicula in the patch.
Summary of the invention
The invention provides a kind of experimental rig and method of looking for the behavior of supporting for research root system vertical direction, utilize this experimental rig can be under the state near nature, research plant rhizosphere Nutrient time loss totally after, its root system is from the close-by examples to those far off responded to the ability of the new nutrient patch of developing, and especially the test plant root system is vertically looked for foster ability.
Technical program of the present invention lies in: a kind ofly look for the experimental rig of the behavior of supporting for research root system vertical direction, comprise for the center drum of transplanting the plant nursery stock and be nested with one heart outer cylinder outside center drum, it is characterized in that: the zone between described center drum and the outer cylinder is disposed with several horizontal interlayers from bottom to top along axis direction, zone between described center drum and the outer cylinder radially is laid with several vertical interlayers, the sector region that is used for the nutrient patch of placement Different Nutrients to be separated out several, the corresponding tapping hole that is laid with towards each sector region on the outer cylinder perisporium, the barrel of described center drum and outer cylinder, horizontal interlayer and vertical interlayer are glass fiber mesh, described glass fibre is coated with agar on the net, intercepts flowing of nutrient in the different sector regions when allowing root system of plant pass glass fiber mesh smoothly.
As a preferred version, described several horizontal interlayers are 4 horizontal interlayers, and described several vertical interlayers are 4 vertical interlayers, described center drum, outer cylinder and horizontal interlayer, 16 sector regions of the common formation of vertical interlayer.
As a preferred version, described tapping hole is arranged on the center of each sector region, hides tinfoil on the described tapping hole, with the loss that prevents that water from dividing.
Another technical scheme of the present invention is: a kind of test method of looking for the behavior of supporting for research root system vertical direction, comprise above-mentioned experimental rig, and it is characterized in that, carry out according to the following steps:
(1) get the identical plant nursery stock of growing way of some, the plant nursery stock be moved in the center drum of above-mentioned experimental rig, and in the sector region of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;
(2) the above-mentioned experimental rig that is placed with plant nursery stock and nutrient patch is divided into some batches;
(3) regularly adopt the method for injector to inject in the sector region of each experimental rig, to keep the skin wet or nutrient solution, inject complete after, adopt tinfoil that tapping hole is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get the mensuration that the interior root system of each sector region carries out physiology and morphological index.
(5) record test data: distribution and the growing state of root system in each sector region of different time sections.
As a preferred version, above-mentioned steps (4) scans each root system image for getting the root system of each sector region by the digital scanning instrument, and applies image analysis software carries out quantitative analysis to each layer root system, carries out simultaneously the mensuration of relative physiologic index.
The invention has the advantages that: experimental rig of the present invention can be under the state near nature, research plant rhizosphere Nutrient time loss totally after, its root system is from the close-by examples to those far off responded to the ability of the new nutrient patch of developing, and especially the test plant root system is vertically looked for foster ability.Its overall structure is simple, and is cheap, draws materials with easy to make.
Description of drawings
Fig. 1 is the structural representation of experimental rig of the present invention.
Among the figure: 1-center drum, 2-outer cylinder, 3-horizontal interlayer, 4-vertically interlayers, 5-sector region, 6-tapping hole.
Embodiment
For above-mentioned feature and advantage of the present invention can be become apparent, embodiment cited below particularly, and cooperation accompanying drawing are described in detail below.
The structural representation of experimental rig of the present invention as shown in Figure 1, the sidepiece of this figure adopt the mode of perspective to represent, with abundant displaying layer of the present invention shelf structure, the other side is the diagram of profile.
Of the present inventionly a kind ofly look for the experimental rig of the behavior of supporting for research root system vertical direction, comprise for the center drum 1 of transplanting the plant nursery stock and be nested with one heart outer cylinder 2 outside center drum, it is characterized in that: the zone between described center drum and outer cylinder 1 and 2 is disposed with several horizontal interlayers 3 from bottom to top along axis direction, zone between described center drum and outer cylinder 1 and 2 radially is laid with several vertical interlayers 4, the sector region 5 that is used for the nutrient patch of placement Different Nutrients to be separated out several, the corresponding tapping hole 6 that is laid with towards each sector region on outer cylinder 2 perisporiums, the barrel of described center drum and outer cylinder, horizontal interlayer 3 and vertical interlayer 4 are glass fiber mesh, described glass fibre is coated with agar on the net, intercepts flowing of nutrient in the different sector regions when allowing root system of plant pass glass fiber mesh smoothly.
As a preferred version, described several horizontal interlayers are 4 horizontal interlayers, and described several vertical interlayers are 4 vertical interlayers, described center drum, outer cylinder and horizontal interlayer, 16 sector regions of the common formation of vertical interlayer.
As a preferred version, described tapping hole is arranged on the center of each sector region, hides tinfoil on the described tapping hole, with the loss that prevents that water from dividing.
A kind of test method of looking for the behavior of supporting for research root system vertical direction of the present invention comprises above-mentioned experimental rig, it is characterized in that, carries out according to the following steps:
(1) get the identical plant nursery stock of growing way of some, the plant nursery stock be moved in the center drum 1 of above-mentioned experimental rig, and in the sector region of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;
(2) the above-mentioned experimental rig that is placed with plant nursery stock and nutrient patch is divided into some batches;
(3) regularly adopt the method for injector to inject in the sector region of each experimental rig, to keep the skin wet or nutrient solution, inject complete after, adopt tinfoil that tapping hole 6 is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get the mensuration that the interior root system of each sector region carries out physiology and morphological index.
(5) record test data: distribution and the growing state of root system in each sector region of different time sections.
As a preferred version, above-mentioned steps (4) scans each root system image for getting the root system of each sector region by the digital scanning instrument, and applies image analysis software carries out quantitative analysis to each layer root system, carries out simultaneously the mensuration of relative physiologic index.
Embodiment is as follows more specifically:
The research China fir is for the foster behavior of looking for of P elements: the China fir nursery stock is moved in the sand culture device inner core (non-treatment region) that is made of two concentric drums, and inner tube wall and interlayer are the fiberglass screen material of aperture 2mm, and China fir main root and fibrous root all can penetrate smoothly.When carrying out the heterogeneous phosphorus processing of soil, apply granular long-acting slow-release phosphorus source patch respectively in every layer of one of them fan-shaped outer region (treatment region), four layers of among do not repeat mutually from the fan-shaped position of selecting.Sand culture matrix is vanadine (Al
2O
3Cross the 28-48 order) ratio and quartz sand mixing with 1.5%, need alumina is carried out dephosphorization treatment before the dress basin, first with clear water flushing alumina to water without muddiness, soak 24h with 0.01mol/L HCl again, with clear water the alumina surface acid is rinsed well more afterwards, soak 24h with 0.01mol/L HCl, so reprocessing is ten times, and is to guarantee the abundant wash-out of the phosphorus in the alumina, air-dry stand-by.Quartz sand is also used clear water and is cleaned to guarantee the abundant wash-out of its phosphorus.The used matrix of the phosphorus source patch KH of variable concentrations
2PO
4Soak to mix sand, utilize effectively Phosphate Sorption and can slowly evenly discharge the characteristic of available phosphorus of vanadine, control cultivation medium to the supply of available phosphorus, the KH that applies
2PO
4Use first dissolved in distilled water, alumina mixes immersion 24h with solution, and the dried sand after the adding quantitatively mixes again.Duration of test carries out dephosphorization element outer normal nutrient solution and moisture supply, the processing of holing in each fan-shaped center from the experimental rig side, and the method that adopts syringe equivalent to inject, supply with moisture or nutrient solution in the guarantee test processing procedure, inject and utilize after complete tinfoil to hide, with the loss that prevents that water from dividing.After the test processing time finishes, device is carried out layering and minute patch processing, get the root system of each fan-shaped patch, scan each root system image by the digital scanning instrument, applies image analysis software carries out quantitative analysis to each layer root system, carries out simultaneously the mensuration of relative physiologic index.
The present invention does not limit to above-mentioned preferred forms, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (5)
1. one kind is used for studying the experimental rig that the root system vertical direction is looked for the behavior of supporting, comprise for the center drum of transplanting the plant nursery stock and be nested with one heart outer cylinder outside center drum, it is characterized in that: the zone between described center drum and the outer cylinder is disposed with several horizontal interlayers from bottom to top along axis direction, zone between described center drum and the outer cylinder radially is laid with several vertical interlayers, the sector region that is used for the nutrient patch of placement Different Nutrients to be separated out several, the corresponding tapping hole that is laid with towards each sector region on the outer cylinder perisporium, the barrel of described center drum and outer cylinder, horizontal interlayer and vertical interlayer are glass fiber mesh, described glass fibre is coated with agar on the net, intercepts flowing of nutrient in the different sector regions when allowing root system of plant pass glass fiber mesh smoothly.
2. according to claim 1ly a kind ofly look for the experimental rig of the behavior of supporting for research root system vertical direction, it is characterized in that: described several horizontal interlayers are 4 horizontal interlayers, described several vertical interlayers are 4 vertical interlayers, described center drum, outer cylinder and horizontal interlayer, 16 sector regions of the common formation of vertical interlayer.
3. according to claim 1ly a kind ofly look for the experimental rig of the behavior of supporting for research root system vertical direction, it is characterized in that: described tapping hole is arranged on the center of each sector region, hides tinfoil on the described tapping hole, with the loss that prevents that water from dividing.
4. one kind is used for the test method that research root system vertical direction is looked for the behavior of supporting, and comprises each described experimental rig among the claim 1-3, it is characterized in that, carries out according to the following steps:
(1) get the identical plant nursery stock of growing way of some, the plant nursery stock be moved in the center drum of above-mentioned experimental rig, and in the sector region of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;
(2) the above-mentioned experimental rig that is placed with plant nursery stock and nutrient patch is divided into some batches;
(3) regularly adopt the method for injector to inject in the sector region of each experimental rig, to keep the skin wet or nutrient solution, inject complete after, adopt tinfoil that tapping hole is sealed, with the loss that prevents that water from dividing;
(4) regularly taking out a collection of experimental rig observes: get the mensuration that the interior root system of each sector region carries out physiology and morphological index;
(5) record test data: distribution and the growing state of root system in each sector region of different time sections.
5. a kind of test method of looking for the behavior of supporting for research root system vertical direction according to claim 4, it is characterized in that: above-mentioned steps (4) is for getting the root system of each sector region, scan each root system image by the digital scanning instrument, applies image analysis software carries out quantitative analysis to each layer root system, carries out simultaneously the mensuration of relative physiologic index.
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| CN102860227A (en) * | 2012-09-11 | 2013-01-09 | 福建农林大学 | Test device and method used for studying root phenotypic plasticity |
| CN102972231A (en) * | 2012-12-24 | 2013-03-20 | 山东省花生研究所 | Peanut culturing device and system as well as authenticating method for drought resistant peanut varieties |
| CN103960038B (en) * | 2014-05-29 | 2017-05-10 | 福建农林大学 | Test device and method for researching root competition strategy |
| CN104303865B (en) * | 2014-10-28 | 2016-08-17 | 云南省农业科学院农业环境资源研究所 | The method measuring kindred plant Competition for Nutrients relation under the conditions of water planting |
| CN104429854A (en) * | 2014-11-27 | 2015-03-25 | 福建农林大学 | Testing device and method for studying how root system reacts to local intermittent pulse nourishing environment |
| CN105165454A (en) * | 2015-09-23 | 2015-12-23 | 天津大学 | Immersed multicompartment submerged plant rhizosphere box |
| CN105432351A (en) * | 2015-12-21 | 2016-03-30 | 福建农林大学 | Device and method for testing mutual influences of plants and soil animals in polluted environment |
| CN105403569A (en) * | 2015-12-21 | 2016-03-16 | 福建农林大学 | Device and method for evaluating the vertical growth avoidance of plant roots from pollutants |
| CN105875392A (en) * | 2016-06-03 | 2016-08-24 | 中铁西北科学研究院有限公司 | Auxiliary device for plant growing and water retaining in arid region |
| CN108513830A (en) * | 2018-07-02 | 2018-09-11 | 贵州大学 | Experimental provision for the research of root system of plant foraging behavior |
| CN108925281B (en) * | 2018-07-30 | 2023-01-10 | 安徽农业大学 | A bucket cultivated in a pot for analyzing heat is to influence of wheat root system distribution state |
| CN114041385B (en) * | 2021-11-15 | 2022-07-15 | 宁波市农业科学研究院 | Research method for root growth and development of vegetable and fruit crops |
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