CN102160504A - Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage - Google Patents

Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage Download PDF

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Publication number
CN102160504A
CN102160504A CN 201110006140 CN201110006140A CN102160504A CN 102160504 A CN102160504 A CN 102160504A CN 201110006140 CN201110006140 CN 201110006140 CN 201110006140 A CN201110006140 A CN 201110006140A CN 102160504 A CN102160504 A CN 102160504A
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soil sampling
rhizosphere soil
chamber
applicable
seedling stage
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田野
刘�东
方升佐
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Nanjing Forestry University
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Nanjing Forestry University
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Priority to CN 201110006140 priority Critical patent/CN102160504A/en
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Abstract

The invention discloses a rhizobox culture device applicable for sampling rhizosphere in a forest seedling stage. The device a seedling growing chamber and a rhizosphere sampling chamber, wherein water-draining holes are arranged on the bottoms of both the seedling growing chamber and the rhizosphere sampling chamber; the two chamber are movably connected through a connecting part and then communicated with each other; isolating meshes are covered on the vertical plane, in which the connecting part is positioned, and on the water-draining holes respectively; and in the rhizosphere sampling chamber, an isolating layer for sampling soil at the positions with different distances from the root is arranged near the vertical plane of the connecting part. The device is suitable for the forest seedling stage with relatively thick root system and can sample microzone soil at the positions with different distances from the root conveniently, effectively and precisely.

Description

A kind of root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage
 
Technical field
The present invention relates to a kind of root box culturing device of rhizosphere micro-region soil sampling, particularly a kind of root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage.
Background technology
Soil nutrient conversion, validity and and microbiologic population's diversity and activity between in the research that concerns, rhizosphere (rhizosphere) has very special and consequence.Rhizosphere is lived by plant a most active soil microcell that root influences, and root system is by absorbing and secretion changes chemistry, physics and the biological property of rhizosphere soil, and then directly influences in the soil nutrient to the validity of plant.A series of result of study shows, rhizosphere processes such as rhizosphere sedimentation and radicula turnover often are accompanied by that rhizospheric microorganism activity, organic matter are decomposed and the enhancing (Frank and Groffman 2009) of available nutrient supply etc., and what can make Soil Nitrogen can be to property increase 6-100%(Herman et al. 2006; Phillips and Fahey 2008).Therefore, when the validity of inquiring into soil nutrient and ecological effect thereof, be necessary to strengthen to the conversion of rhizosphere soil nutrient and the research of micropopulation body characteristics.
Agriculturally, because crops are generally draft, be main with fibrous root, be easy to control, adopting root case or root bag to carry out analog study (Liu Zhiyu etc. 1997) aspect the rhizosphere micro-region more, the plant district of planting is very narrow and small.And aspect forestry, because fine-root is thick, it is wide to distribute, and restive, the application of the traditional root case on the agricultural is subjected to certain restriction, and rhizosphere soil sampling still more at present adopts the extensive collecting method (Zhang Jinlong etc. 2003) of shaking off.This method is owing to be subjected to condition effect such as soil types, humidity and root system of plant kind, the scope of its sampling is difficult to control, there is very big defective aspect the sampling accuracy, and sample time is long, and cause the residual of part root system tissue easily, the rhizosphere soil nutrient transforms and the assay determination of microorganism feature thereby have influence on, and causes result of study to lack accuracy.In addition, owing to have no idea the soil of distance root different levels is distinguished, also can't the studying of rhizosphere process apart from Gradient Effect.Therefore, lack the bottleneck that suitable research method and means become nutrient conversion of the little territory of restriction forestry rhizosphere soil and microbiologic properties research.
Summary of the invention
Goal of the invention:At the deficiency on the existing research means, the technical problem to be solved in the present invention provides a kind of forest seedling stage that root system is comparatively thick of being applicable to, can make things convenient for, effectively and exactly carry out apart from the root box culturing device of root different distance micro-region soil sampling.
Technical scheme:In order to solve the problems of the technologies described above, the present invention adopts following technological means:
A kind of root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage, comprise that the bottom is equipped with the seedling growth chamber and the rhizosphere soil sampling chamber of osculum, two Room communicate after flexibly connecting by connecting portion, cover on connecting portion place facade and the osculum and be covered with separation net, rhizosphere soil sampling is indoor to be provided with at least 2 interlayers near connecting portion facade place.
It is that 30 μ m, thickness are the polyamide microporous barrier of 90 μ m that above-mentioned separation net adopts the aperture.
Above-mentioned interlayer is made up of interlayer framework and separation membrane, and the interlayer frame size is as the criterion just to embed the rhizosphere soil sampling chamber, and the interlayer body thickness is according to the sampling requirement setting, and separation membrane covers and is overlying on the interlayer framework, away from the one side of seedling growth chamber.
It is that 30 μ m, thickness are the polyamide microporous barrier of 90 μ m that above-mentioned separation membrane adopts the aperture.
Above-mentioned interlayer body thickness is 1mm~4mm.
The width of above-mentioned rhizosphere soil sampling chamber is fixed as 4~8cm.
The width of above-mentioned rhizosphere soil sampling chamber is preferably 5cm.
The diameter of above-mentioned osculum is 10mm, and pitch of holes is 50 ~ 80mm.
The casing of above-mentioned seedling growth chamber and rhizosphere soil sampling chamber is light tight.
The size of above-mentioned seedling growth chamber can according to root system size and how many adjustment of nursery stock quantity of the nursery stock of planting.
Beneficial effect:During the actual use of a kind of root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of the present invention,, the nursery stock of test usefulness is planted on request or cuttage is cultivated in seedling growth is indoor at seedling growth chamber and the indoor soil of all inserting of rhizosphere soil sampling.This device has following good result:
1. enlarge the spatial dimension of seedling growth chamber, not only can adapt to thick, the widespread characteristic of fine-root, and can mix the constructed experiment of aspects such as friendship, the compound operation of self-employed tree cultivator.
2. adopting the aperture is that 30 μ m, thickness are that the polyamide microporous barrier of 90 μ m is directly tightened to be attached on connecting portion and the interlayer framework physical isolation is carried out in seedling growth chamber and different levels interlayer, microporous barrier can effectively stop penetrating of radicula and Gen Mao, root system is controlled at the indoor growth of seedling growth, but can influence the migration of moisture, nutrient, root exudates and edaphon at interlayer.The thickness of microporous barrier can be ignored with respect to compartment thickness, and is extremely small to the influence of sampling accuracy.
3. select the interlayer of different-thickness according to the test needs, when sampling, pull down the sampling room with rhizosphere, take out the interlayer of different levels one by one, can obtain very easily apart from the soil specimen of root different distance, not only greatly simplify the sampling method of rhizosphere soil, and increase the accuracy of sampling and the integrality of soil layer, sampling can not cause because the long change that causes the biochemical characteristic of soil sample time rapidly.
Description of drawings
Fig. 1 is a kind of perspective view that is applicable to the root box culturing device of forest rhizosphere soil sampling in seedling stage of the present invention;
Fig. 2 is that a kind of stereochemical structure of the root box culturing device of forest rhizosphere soil sampling in seedling stage that is applicable to of the present invention splits schematic diagram;
Fig. 3 is the positive three-dimensional cross-sectional view of root box culturing device;
Fig. 4 is that schematic diagram is looked on the right side of interlayer among Fig. 2.
Embodiment
Fig. 1 ~ Fig. 4, is elaborated to the specific embodiment of the present invention with reference to above-mentioned accompanying drawing in conjunction with having provided a kind of root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage.
Embodiment 1:
A kind of root box culturing device of forest rhizosphere soil sampling in seedling stage that is applicable to of the present invention comprises a dismountable rectangular box container, and this box container is made up of the seedling growth chamber 1 on the right side among Fig. 1 and the rhizosphere soil sampling chamber 2 on the left side.
Seedling growth chamber 1 is to be surrounded by 11,1 side plate 12 of 2 side plates and 1 base plate 13 to form, sheet material all adopts the thick opaque black lucite of 3mm, 2 side plates 11 are parallel to each other, the height of side plate 11 and side plate 12 equates, the transverse width of side plate 11 and base plate 13 equates that vertical width of side plate 12 and base plate 13 equates.Several osculums 14 evenly are set on the base plate 13, and the diameter of osculum 14 is 10mm, and pitch of holes is advisable with 50 ~ 80mm.Adopt the double strength glass glue bond to form between side plate 11, side plate 12 and the base plate 13.The size of seedling growth chamber 1 can according to root system size and how many adjustment of nursery stock quantity of cultivation nursery stock.
The connecting portion 3 of seedling growth chamber 1 and rhizosphere soil sampling chamber 2 is made up of 2 lucite bars 31 of front and back, the lucite bar 32 of bottom and the lucite bar 33 at top, the all thick 3mm of lucite bar, wide 10mm, length is advisable just to embed seedling growth chamber 1.Before and after 2 lucite bars 31 and the lucite bar 32 of bottom respectively stay the 5mm width, adopt double strength glass glue that other 5mm width is attached to inside, left side, seedling growth chamber, the lucite bar 33 at top adopts the double strength glass stickers to be attached to the top of 2 lucite bars 31 of front and back.
Rhizosphere soil sampling chamber 2 is to be surrounded by 21,1 side plate 22 of 2 side plates and 1 base plate 23 to form, sheet material all adopts the thick opaque black lucite of 3mm, 2 side plates 21 are parallel to each other, the height of side plate 21 and side plate 22 equates with the height of the side plate 11 of seedling growth chamber 1, the transverse width of side plate 21 and base plate 23 equates, be advisable with 55mm, vertical width of the vertical width of side plate 22 and base plate 23 and the side plate 12 of seedling growth chamber 1 equates.Delegation's osculum 24 vertically evenly is set on the base plate 23, and the diameter of osculum 24 is 10mm, and pitch of holes is advisable with 50 ~ 80mm.Adopt the double strength glass glue bond to form between side plate 21, side plate 22 and the base plate 23.
Adopt the polyamide microporous barrier to separate between seedling growth chamber 1 and the rhizosphere soil sampling chamber 2, this polyamide microporous barrier thickness is that 90 μ m, aperture are 30 μ m.Earlier the polyamide microporous barrier is directly tightened on the plane of the connecting portion 3 of seedling growth chamber 1 during making, with super glue the polyamide microporous barrier is glued on the connecting portion 3 again, treat glue do after the polyamide microporous barrier that the periphery is unnecessary cut off and promptly form the separation net 4 that is fixed on the connecting portion 3.
Side (right side) near seedling growth chamber 1 in rhizosphere soil sampling chamber 2 is provided with the interlayer 5 that is used for more than 1 apart from the soil sample of root different distance as required.Interlayer 5 is made up of interlayer framework 51 and the separation membrane 52 that is attached on the interlayer framework 51.Interlayer framework 51 adopts the poly (methyl methacrylate) plate of different-thickness that the core excision is made, the size of interlayer framework 51 is as the criterion just to embed the rhizosphere soil sampling chamber, and the thickness of interlayer framework 51 is according to sampling requirement setting (generally can be set to 1 mm, 2 mm or 4 mm).It is that 30 μ m, thickness are that the polyamide microporous barrier of 90 μ m directly is attached on the interlayer framework 51 that separation membrane 52 adopts apertures, earlier the polyamide microporous barrier is directly tightened on the side away from seedling growth chamber 1 of interlayer framework 51 during making, with super glue the polyamide microporous barrier is glued on the interlayer framework again, treat glue do after the polyamide microporous barrier that the periphery is unnecessary cut off and get final product.Because of the thickness of separation membrane 52 only is 90 μ m, much smaller than the thickness of interlayer 5, the influence of being close to mutually between the interlayer when it uses reality can be ignored, and is unlikely to have influence on the precision of different levels soil sample.
Adopt a kind of root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of the present invention, method and process during its actual use are as follows:
At first, seedling growth chamber 1 and rhizosphere soil sampling chamber 2 is chimeric by connecting portion 3, twine so that casing is fixed with wide adhesive tape in the casing outside.Interlayer 5 inserted rhizosphere soil sampling chambers 2 and make be close to separation net 4, finish the assembling of root box culturing device.Sticking one deck aperture then on the osculum of the base plate of seedling growth chamber 1 and rhizosphere soil sampling chamber 2 is that 30 μ m, thickness are the polyamide microporous barrier of 90 μ m, passes from osculum to prevent root system.
The bulk density of soil of Tian Zhuaning and casing volume calculate with the soil amount as required, and soil was respectively charged in seedling growth chamber 1 and the rhizosphere soil sampling chamber 2 after grinding the 2mm sieve in advance.Be subjected to squeeze distortion for fear of separation membrane, need when banketing to different chamber simultaneously progressively layering pack into.After dress soil finishes, seedling growth chamber 1 in, carry out sowing, the cuttage of nursery stock or plant, water the permeable nursery stock cultivation of carrying out then according to the seeding row spacing that designs or mixed friendship mode.After nursery stock begins growth, it is that the obstruct of the polyamide microporous barrier of 30 μ m is limited in the indoor stretching, extension of seedling growth that the growth of its root system is subjected to the aperture, thereby the root layer that forms densification is assembled on the right side at separation net 4, separation net 4 is root pedosphere face, the soil body of contact separation net 4 is rhizosphere soil in the rhizosphere soil sampling chamber 2, soil in separation net 4 different distance interlayers is apart from the soil of root different distance, can be used for studying the rhizosphere process apart from Gradient Effect.
When the nursery stock cultivation stage finishes, in the time of need carrying out soil sample, scratch the adhesive tape of tank surface from the junction with cutter, rhizosphere soil sampling chamber 2 is pulled down, take out the interlayer of different levels then one by one, collection soil is wherein poured out whole soil with soil sample chamber left-hand thread at last as the soil specimen of distance root different levels, get outermost soil as non-rhizosphere soil sample, can realize the separation collection of rhizosphere micro-region different levels soil.
Embodiment 2
A kind of root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage, comprise that the bottom is equipped with the seedling growth chamber 1 and the rhizosphere soil sampling chamber 2 of osculum, two Room communicate after flexibly connecting by connecting portion 3, cover on connecting portion 3 place facades and the osculum and be covered with separation net, in the rhizosphere soil sampling chamber 2, be provided with at least 2 interlayers 5 near connecting portion 3 facade places.It is that 30 μ m, thickness are the polyamide microporous barrier of 90 μ m that described separation net adopts the aperture.Described interlayer 5 is made up of interlayer framework 51 and separation membrane 52, and the interlayer frame size is as the criterion just to embed rhizosphere soil sampling chamber 2, and the interlayer body thickness is according to the sampling requirement setting, and separation membrane 52 covers the one side that is overlying on the interlayer framework 51 away from seedling growth chamber 1.It is that 30 μ m, thickness are the polyamide microporous barrier of 90 μ m that described separation membrane 52 adopts the aperture.Described interlayer body thickness is 1mm~4mm.The width of described rhizosphere soil sampling chamber 2 is fixed as 4~8cm.The width of described rhizosphere soil sampling chamber 2 is preferably 5cm.The diameter of described osculum is 10mm, and pitch of holes is 50 ~ 80mm.The casing of described seedling growth chamber 1 and rhizosphere soil sampling chamber 2 is light tight.The size of described seedling growth chamber 1 can according to root system size and how many adjustment of nursery stock quantity of cultivation nursery stock.

Claims (10)

1. root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage, it is characterized in that comprising that the bottom is equipped with seedling growth chamber (1) and rhizosphere soil sampling chamber (2) of osculum, two Room communicate after flexibly connecting by connecting portion (3), cover on connecting portion (3) place facade and the osculum and be covered with separation net, be provided with interlayer (5) near connecting portion (3) facade place in rhizosphere soil sampling chamber (2).
2. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 1, it is characterized in that it is that 30 μ m, thickness are the polyamide microporous barrier of 90 μ m that described separation net adopts the aperture.
3. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 1, it is characterized in that described interlayer (5) is made up of interlayer framework (51) and separation membrane (52), the interlayer frame size is as the criterion just to embed rhizosphere soil sampling chamber (2), the interlayer body thickness is according to the sampling requirement setting, and separation membrane (52) covers and is overlying on the last one side away from seedling growth chamber (1) of interlayer framework (51).
4. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 3, it is characterized in that it is that 30 μ m, thickness are the polyamide microporous barrier of 90 μ m that described separation membrane (52) adopts the aperture.
5. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 3, it is characterized in that described interlayer body thickness is 1mm~4mm.
6. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 1, it is characterized in that the width of described rhizosphere soil sampling chamber (2) is fixed as 4~8cm.
7. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 6, it is characterized in that the width of described rhizosphere soil sampling chamber (2) is preferably 5cm.
8. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 1, the diameter that it is characterized in that described osculum is 10mm, and pitch of holes is 50 ~ 80mm.
9. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 1, it is characterized in that the casing of described seedling growth chamber (1) and rhizosphere soil sampling chamber (2) is light tight.
10. according to the described root box culturing device that is applicable to forest rhizosphere soil sampling in seedling stage of claim 1, it is characterized in that of root system size and nursery stock quantity how many adjustment of the size of described seedling growth chamber (1) according to the nursery stock of planting.
CN 201110006140 2011-01-13 2011-01-13 Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage Pending CN102160504A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103960038A (en) * 2014-05-29 2014-08-06 福建农林大学 Test device and method for researching root competition strategy
CN105115780A (en) * 2015-08-24 2015-12-02 成都中医药大学 Plant rhizosphere soil and rhizosphere exudate collecting device and method
CN106688666A (en) * 2016-11-29 2017-05-24 暨南大学 Plant rhizosphere box and application thereof in micro-domain research of wet land plant rhizosphere
CN107976330A (en) * 2017-11-21 2018-05-01 东北师范大学 The collection device in situ and method of secretion from root system of plant in pot and rhizosphere soil
CN105841995B (en) * 2016-03-18 2018-06-08 中国科学院亚热带农业生态研究所 The sampler and sampling method of a kind of plant rhizosphere and non-rhizosphere soils
CN108845090A (en) * 2018-06-01 2018-11-20 重庆工业职业技术学院 A method of probing into reservoir drawdown band typical vegetation rhizosphere micro-region mercury activating effect

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CN1869631A (en) * 2006-05-31 2006-11-29 浙江大学 Sampling device of rhizosphere micro-region soil
CN101082547A (en) * 2007-07-12 2007-12-05 浙江大学 Plants root box culturing device used for rhizosphere soil sampling
CN202035323U (en) * 2011-01-13 2011-11-16 南京林业大学 Root box culture device applicable to rhizosphere soil sampling in forest seedling stage

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10229758A (en) * 1997-02-17 1998-09-02 Iseki & Co Ltd Container for nursery
JP2001197840A (en) * 2000-01-19 2001-07-24 Iseki & Co Ltd Watering apparatus
CN1869631A (en) * 2006-05-31 2006-11-29 浙江大学 Sampling device of rhizosphere micro-region soil
CN101082547A (en) * 2007-07-12 2007-12-05 浙江大学 Plants root box culturing device used for rhizosphere soil sampling
CN202035323U (en) * 2011-01-13 2011-11-16 南京林业大学 Root box culture device applicable to rhizosphere soil sampling in forest seedling stage

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103960038A (en) * 2014-05-29 2014-08-06 福建农林大学 Test device and method for researching root competition strategy
CN105115780A (en) * 2015-08-24 2015-12-02 成都中医药大学 Plant rhizosphere soil and rhizosphere exudate collecting device and method
CN105115780B (en) * 2015-08-24 2018-09-28 成都中医药大学 Plant rhizosphere soil and root rhizosphere secreta collection device and collection method
CN105841995B (en) * 2016-03-18 2018-06-08 中国科学院亚热带农业生态研究所 The sampler and sampling method of a kind of plant rhizosphere and non-rhizosphere soils
CN106688666A (en) * 2016-11-29 2017-05-24 暨南大学 Plant rhizosphere box and application thereof in micro-domain research of wet land plant rhizosphere
CN106688666B (en) * 2016-11-29 2020-11-06 暨南大学 Plant rhizosphere box and application thereof in wetland plant rhizosphere micro-domain research
CN107976330A (en) * 2017-11-21 2018-05-01 东北师范大学 The collection device in situ and method of secretion from root system of plant in pot and rhizosphere soil
CN108845090A (en) * 2018-06-01 2018-11-20 重庆工业职业技术学院 A method of probing into reservoir drawdown band typical vegetation rhizosphere micro-region mercury activating effect

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Application publication date: 20110824