CN104007094B - A kind of blade master passive chlorophyll fluorescence long-term sequence synergistic observation system - Google Patents
A kind of blade master passive chlorophyll fluorescence long-term sequence synergistic observation system Download PDFInfo
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- CN104007094B CN104007094B CN201410220579.2A CN201410220579A CN104007094B CN 104007094 B CN104007094 B CN 104007094B CN 201410220579 A CN201410220579 A CN 201410220579A CN 104007094 B CN104007094 B CN 104007094B
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Abstract
A kind of blade master passive chlorophyll fluorescence long-term sequence synergistic observation system, this system is made up of simultaneous observation frame, spectra collection equipment, actively fluorescent collecting equipment, photosynthetically active radiation collecting device and five parts of main frame；Spectra collection equipment, actively fluorescent collecting equipment, photosynthetically active radiation collecting device are arranged on simultaneous observation frame, spectra collection equipment, actively fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame are all coupled together with main frame by data line, are realized the control of motor on spectrum, Change of Chlorophyll Fluorescence Kinetics Parameters, the collection of photosynthetically active radiation data and simultaneous observation frame by main frame.This blade master passive chlorophyll fluorescence long-term sequence synergistic observation concept of system science, simple in construction, it is simple to carry.It is measured object and measures the concordance of environment when preferably ensure that main passive fluorescence measurement, system has stronger stability, can carry out the synergistic observation of long-term sequence.
Description
Technical field
The present invention relates to a kind of blade master passive chlorophyll fluorescence long-term sequence synergistic observation system, it is that in geography, research is planted
By the integrated system of spectrum Yu vegetation physiological relation, blade can be carried out passive sunlight-induced chlorophyll fluorescence (Solar-induced
Chlorophyll fluorescence, SIF) and the long-term sequence synergistic observation of active Change of Chlorophyll Fluorescence Kinetics Parameters, it is used for setting up
Play passive fluorescence and actively Change of Chlorophyll Fluorescence Kinetics Parameters and the physiological relation of vegetation, belong to physiographical systems technical field.
Background technology
Photosynthesis is the physiological process that vegetation is the most key, not only affect the material such as carbon, water circulation, and can quickly,
Directly reflect the growth conditions of plant and by stress state.The electromagnetism that tradition remote sensing is reflected by vegetation, radiates and scatter
The biophysical parameters of ripple information inverting vegetation and biochemical parameters, such as leaf area index, chlorophyll content etc., but at present
Also lack effective detection plant photosynthesis information and the remote sensing by stress state.Vegetation chlorophyll fluorescence because and vegetation
Photosynthesis is closely related, is considered as " probe " of plant physiology state after deliberation, with the external environment residing for vegetation growth
Closely bound up, the stress state that chlorophyll fluorescence is applied to detect vegetation has the biggest potentiality.Actively fluorescence measurement is to pass through
Actively launching pulse to obtain the Change of Chlorophyll Fluorescence Kinetics Parameters of vegetation leaf scale, the physiological Study for vegetation has critically important reference
Meaning.SIF extracts fluorescence information by algorithm from the apparent reflectance spectrum of sun incident light spectrum and target, can pass through remote sensing technique
Obtain, the physiological status observation of large area surface vegetation can be realized.At present low latitude, the whole world SIF the most studied, but from spectrum
Whether the SIF extracted is the Correct that vegetation truly launches fluorescence, and can SIF correctly reflect the physiological status of vegetation, also need
Verify further and determine.Additionally precision and the effectiveness of many kinds of extracting method of SIF is also required to be analyzed.Therefore need one
Can measure the system of main passive fluorescence, this system is it is ensured that the measured target of main passive fluorescence measurement keeps consistent with environment simultaneously
Property, and can facilitate, stably carry out long time-ordered measurement.We overlap blade master long-time sequence of passive chlorophyll fluorescence
Row synergistic observation system is just for light-duty, the automatic simultaneous observation system of problems above exploitation.This cover system includes
Spectra collection equipment, actively fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame and control main process equipment five
Part is constituted, and blade target stably can carry out master's passive fluorescence simultaneous observation of long-term sequence, in order to study passive fluorescence
Relation physiological with active Change of Chlorophyll Fluorescence Kinetics Parameters and vegetation.
Summary of the invention
(1) purpose:
It is an object of the invention to provide a kind of blade master passive chlorophyll fluorescence long-term sequence synergistic observation system.Which ensure master
External environment, blade interior physiology that passive fluorescence obtains are all consistent, so that it is guaranteed that both have strict corresponding relation.System
There is the convenience of good stability and operation, the long-term sequence simultaneous observation of main passive fluorescence data can be realized.
(2) technical scheme
The present invention is a kind of blade master passive chlorophyll fluorescence long-term sequence synergistic observation system (Fig. 1).Whole system is by real
Existing function is made up of five parts: simultaneous observation frame (Fig. 2), spectra collection equipment (Fig. 3), actively fluorescent collecting equipment (figure
4), photosynthetically active radiation collecting device (Fig. 5), main frame.Position annexation between them is: spectra collection equipment,
Actively fluorescent collecting equipment, photosynthetically active radiation collecting device are arranged on simultaneous observation frame, spectra collection equipment, actively fluorescence
Collecting device, photosynthetically active radiation collecting device, simultaneous observation frame are all coupled together with main frame by data line, by master
Machine realizes the control of the motor on spectrum, Change of Chlorophyll Fluorescence Kinetics Parameters, the collection of photosynthetically active radiation data and simultaneous observation frame.
The synergistic observation of the main passive fluorescence long-term sequence of the integration realization of above equipment.
Described simultaneous observation frame (Fig. 2) is to be assembled by rustless steel, and four root type steel compositions are cross as at the bottom of simultaneous observation frame
Seat, simultaneous observation frame body frame is spliced by many root type steel, between the object disposing platform of rectangle plate shape, simultaneous observation frame are installed
Body frame upper end connects observation part, can adjust observation Partial Height, and observation unit is divided and included motor, reference plate pallet, HR4000
Montant and the sample platform of rectangle plate shape is fixed with PAM2500 optical fiber.HR4000 spectrogrph fibre-optical probe is in observed object
Move back and forth between above position and diffuse-reflectance reference plate position, it is achieved that adopting of sun incident light spectrum and the apparent reflectance spectrum of target
Collect (Fig. 6) and provide the stable environment of spectrum and Change of Chlorophyll Fluorescence Kinetics Parameters synchronous acquisition (Fig. 7).
Described spectra collection equipment (Fig. 3) includes calorstat and built-in HR4000 spectrogrph, diffuse-reflectance reference plate, can be real
Existing sun incident light spectrum and the measurement of the apparent reflectance spectrum of target, to extract SIF, calorstat can ensure that HR4000 spectrogrph is long
The steady operation of time.This calorstat is case structure, which is provided with HR4000 data wire, 1HR4000 spectrogrph optical fiber,
Calorstat data line interface, calorstat optical fiber interface, calorstat power interface, calorstat power supply changeover device and calorstat power line；
This diffuse-reflectance reference plate is small circular plate, and its surface is provided with lambert's body specific coatings that reflectance is 99%.
Described active fluorescent collecting equipment (Fig. 4) is PAM2500 luminoscope, can obtain the fluorescence kinetics ginseng of blade target
Number.Which is provided with PAM2500 data wire, PAM2500 optical fiber, PAM2500 data line interface, PAM2500 optical fiber connect
Mouth and PAM2500 fibre-optical probe.
Described photosynthetically active radiation collecting device (Fig. 5) is PAR sensor and PAR main frame, can obtain current photosynthetic effectively
Radiation value.
Described main frame is a computer, is provided with HR4000 and controls software, PAM2500 and control software, PAR sensor control
Software processed, has arranged acquisition method and the flow process of data above.
Principle and the operating mode brief introduction of the present invention are as follows: PAM2500 luminoscope and HR4000 spectrogrph are passed through to synchronize by the present invention
Observation platform carries out integrated, it is provided that a kind of blade master passive chlorophyll fluorescence long-term sequence synergistic observation system.System passes through main frame
Control the operation of motor on simultaneous observation frame, make HR4000 spectrogrph fibre-optical probe observed object and diffuse-reflectance reference plate it
Between switch over, it is achieved sun incident light spectrum and the collection of the apparent reflectance spectrum of observed object, when spectra collection object is blade,
Gather active fluorescence parameter and the apparent reflectance spectrum of blade and current photosynthetically active radiation value simultaneously.By main frame arrange spectrum,
Fluorescence, the passive chlorophyll fluorescence of master so that identical observed object to carry out long-term sequence that automatically gathers of photosynthetically active radiation are seen
Survey.
(3) advantage, effect
This blade master passive chlorophyll fluorescence long-term sequence synergistic observation concept of system science, simple in construction, it is simple to carry.It
Measuring object when preferably ensure that main passive fluorescence measurement and measure the concordance of environment, system has stronger stability, energy
Carry out the synergistic observation of long-term sequence.
Accompanying drawing explanation
Fig. 1 system general illustration
Fig. 2 simultaneous observation frame schematic diagram
Fig. 3 spectra collection equipment schematic diagram
Fig. 4 actively fluorescent collecting equipment schematic diagram
Fig. 5 light and Net long wave radiation collecting device schematic diagram
Fig. 6 simultaneous observation frame motor runs schematic diagram
Fig. 7 simultaneous observation schematic diagram
In figure, symbol description is as follows:
1 simultaneous observation frame base；2 simultaneous observation frame body frames；3 object disposing platforms；4 sample platform；5 reference plate pallets；6HR4000
Montant fixed by spectrogrph optical fiber；Montant fixed by 7PAM2500 optical fiber；8 motors；9 motor cable lines；10 motor main frames；11
Motor supplying cell；12 motor power-supply wires；13 motor host data lines；14 calorstats；15HR4000 spectrogrph；16
HR4000 data wire；17HR4000 spectrogrph optical fiber；18 calorstat data line interfaces；19 calorstat optical fiber interfaces；20 is permanent
Incubator power interface；21 calorstat power supply changeover devices；22 calorstat power lines；23 diffuse-reflectance reference plates；24PAM2500
Luminoscope；25PAM2500 data wire；26PAM2500 optical fiber；27PAM2500 data line interface；28PAM2500 light
Fine interface；29PAR main frame；30PAR sensor；31PAR host data line；32 main frames；33HR4000 spectrogrph light
Fine probe；34PAM2500 fibre-optical probe；35 observed objects.
Detailed description of the invention
As shown in Fig. 1 Fig. 7, one blade master of the present invention passive chlorophyll fluorescence long-term sequence synergistic observation system is by spectrum
Collecting device, actively fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame, 32 5 part groups of main frame
Become.Position annexation between them is: spectra collection equipment, actively fluorescent collecting equipment, photosynthetically active radiation collection set
For being arranged on simultaneous observation frame, spectra collection equipment, actively fluorescent collecting equipment, photosynthetically active radiation collecting device, synchronization
Observation platform is all coupled together with main frame by data line, realizes spectrum, Change of Chlorophyll Fluorescence Kinetics Parameters, PAR by main frame 32
The control of the motor in data acquisition and simultaneous observation frame.The main passive fluorescence long-term sequence of the integration realization of above equipment
Synergistic observation.
Described spectra collection equipment is HR4000 spectrogrph 15 and adnexa thereof, and this spectrogrph has 0.02nm spectral resolution,
Spectral region covers 700-800nm, can extract the fluorescent emission peak value being positioned at 760nm.HR4000 spectrogrph 15 is put
It is placed in calorstat 14, realizes signal by calorstat data line interface 18 and calorstat optical fiber interface 19 and transmit.Calorstat
14 make HR4000 spectrogrph 15 measure during temperature constant at 23 DEG C, calorstat 14 is through calorstat power line 22 and calorstat
Power supply changeover device 21 is powered by external power supply.HR4000 spectrogrph fibre-optical probe 33 is fixed on the HR4000 on simultaneous observation frame
Montant 6 fixed by spectrogrph optical fiber, and diffuse-reflectance reference plate 23 is fixed in simultaneous observation frame reference plate pallet 5.Main frame 32 passes through
Motor host data line 13 controls the operation of motor 8 on simultaneous observation frame, makes the HR4000 spectrogrph fibre-optical probe 33 can be complete
Paired observation target 35 and the switching of diffuse-reflectance reference plate 23, obtain sun incident light spectrum and the reflectance spectrum of observed object respectively,
Sunlight-induced chlorophyll fluorescence is calculated by existing algorithm.This calorstat 14 is case structure, which is provided with HR4000 number
According to line 16, HR4000 spectrogrph optical fiber 17, calorstat data line interface 18, calorstat optical fiber interface 19, calorstat power supply
Interface 20, calorstat power supply changeover device 21 and calorstat power line 22.
Described active fluorescent collecting equipment is PAM2500 luminoscope 24 and adnexa thereof, PAM2500 luminoscope fibre-optical probe 34
Keeping relative position relation constant with observed object 35, PAM2500 luminoscope fibre-optical probe 34 obtains the master of observed object 35
Dynamic fluorescence signal, signal is transferred to main frame 32 through PAM2500 data wire 25, and PAM2500 luminoscope 24 which is provided with
PAM2500 data wire 25, PAM2500 optical fiber 26, PAM2500 data line interface 27 and PAM2500 optical fiber interface 28；
Its built-in power can complete the time-ordered measurement of 8 hours.
Described photosynthetically active radiation collecting device is made up of PAR sensor 30 and PAR main frame 29, No.5 cell group power,
The PAR data obtained are stored in PAR main frame 29, can import main frame 32 by PAR host data line 31.
Described simultaneous observation frame is assembled by stainless steel material, and four root type steel compositions are cross as simultaneous observation frame base 1,
Simultaneous observation frame body frame 2 is spliced by many root type steel, and the glove being provided with rectangle plate shape in the middle part of simultaneous observation frame body frame 2 is put down
Platform 3, can place pertinent instruments and equipment, and simultaneous observation frame body frame 2 upper end connects observation part, and observation Partial Height can be adjusted
Joint, observation unit is divided and is included that montant 6 fixed by motor 8, reference plate pallet 5, HR4000 optical fiber, PAM2500 optical fiber is fixed perpendicular
Bar 7 and the sample platform 4 of rectangle plate shape, montant 6 fixed by HR4000 optical fiber, PAM2500 optical fiber is fixed montant 7 and is used for
Fixing HR4000 spectrogrph fibre-optical probe 33, PAM2500 luminoscope fibre-optical probe 34, sample platform 4 is used for placing observation
Target 35, places diffuse-reflectance reference plate 23 in reference plate pallet 5, PAR sensor 30 is arranged on simultaneous observation frame upper horizontal
Position.Motor main frame 10 is connected with motor 8 by motor cable line 9, can run motor 8 and control, it is possible to by electricity
Machine host data wire 13 is connected to main frame 32, and the operation to motor 8 automatically sets.Motor 8 rotates so that HR4000
Spectrogrph fibre-optical probe 33 object of observation switches between diffuse-reflectance reference plate 23 and observed object 35.Motor 8 is step
Enter motor, motor supplying cell 11 be powered, it is possible to achieve motor 8 works long hours.
Described main frame 32 be one be provided with HR4000 control software, PAM2500 control software, PAR sensor control soft
Part and the computer of motor control software, by motor host data line 13 arrange the initial position of motor 8, traffic direction,
The speed of service, target location and diffuse-reflectance reference plate 23 position time out and cycle of operation, it is achieved HR4000 optical fiber is solid
Determine the montant 6 automatic switchover (Fig. 6) in target location and diffuse-reflectance reference plate 23 position.By HR4000 data wire 16,
PAM2500 data wire 25, PAR host data line 31 are respectively provided with PAM2500 luminoscope 24 and HR4000 spectrogrph
The simultaneous observation cycle of 15 pairs of observed objects 35, and the data collection cycle of PAR sensor 30, it is achieved spectrum, actively
Fluorescence parameter, the synergistic observation of PAR.
Claims (1)
1. a blade master passive chlorophyll fluorescence long-term sequence synergistic observation system, it is characterised in that: this system is by synchronizing
Observation platform, spectra collection equipment, actively fluorescent collecting equipment, photosynthetically active radiation collecting device and five, main frame part composition；
Spectra collection equipment, actively fluorescent collecting equipment, photosynthetically active radiation collecting device are arranged on simultaneous observation frame, spectra collection
Equipment, actively fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame all pass through data line with main frame even
Pick up, realized on spectrum, Change of Chlorophyll Fluorescence Kinetics Parameters, the collection of photosynthetically active radiation data and simultaneous observation frame by main frame
The control of motor；
Described simultaneous observation frame is to be assembled by rustless steel, and four root type steel compositions are cross as simultaneous observation frame base, synchronize
Observation platform body frame is spliced by many root type steel, between the object disposing platform of rectangle plate shape, simultaneous observation frame body frame upper end are installed
Connecting observation part, it highly can adjust, and observation unit is divided and included that motor, reference plate pallet, optical fiber fix montant and rectangular slab
The sample platform of material shape；Spectrogrph fibre-optical probe above Observed Position and diffuse-reflectance reference plate position between move back and forth,
Achieve sun incident light spectrum and the collection of the apparent reflectance spectrum of target, and provide spectrum and Change of Chlorophyll Fluorescence Kinetics Parameters synchronous acquisition
Stable environment；
Described spectra collection equipment includes the diffuse-reflectance reference plate outside the built-in spectrogrph of calorstat, calorstat and calorstat, energy
Realizing sun incident light spectrum and the measurement of the apparent reflectance spectrum of target, to extract SIF, calorstat can ensure that spectrogrph is long
Steady operation；This calorstat is case structure, which is provided with data wire, spectrogrph optical fiber, calorstat data line interface, constant temperature
Case optical fiber interface, calorstat power interface, calorstat power supply changeover device and calorstat power line；This diffuse-reflectance reference plate is roundlet
Plate, its surface is provided with lambert's body specific coatings that reflectance is 99%；
Described active fluorescent collecting equipment is luminoscope, can obtain the Change of Chlorophyll Fluorescence Kinetics Parameters of blade target, which is provided with data wire,
Optical fiber, data line interface, optical fiber interface and fibre-optical probe；
Described photosynthetically active radiation collecting device is PAR sensor and PAR main frame, can obtain current photosynthetically active radiation value；
Described main frame is a computer, is provided with HR4000 and controls software, PAM2500 and control software, PAR sensor control
Software processed, has arranged acquisition method and the flow process of data above.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| IT201900020174A1 (en) * | 2019-10-31 | 2021-05-01 | Consiglio Nazionale Ricerche | CALIBRATION DEVICE FOR MEASURING THE FLUORESCENCE OF CHLOROPHYLL INDUCED BY SUNLIGHT |
Families Citing this family (5)
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| CN105486673B (en) * | 2016-01-27 | 2020-03-10 | 北京师范大学 | Chlorophyll fluorescence automatic monitoring system |
| CN105548122B (en) * | 2016-01-27 | 2020-04-17 | 北京师范大学 | Chlorophyll fluorescence time-sharing monitoring system |
| CN105842220A (en) * | 2016-05-26 | 2016-08-10 | 伯格森(北京)科技有限公司 | Vegetation fluorescence time sequence measuring system and method |
| CN109580558A (en) * | 2017-09-28 | 2019-04-05 | 中国科学院地理科学与资源研究所 | A kind of sunlight-induced vegetation fluorescence measuring systems suitable for different canopy layers height |
| CN113008372A (en) * | 2021-03-08 | 2021-06-22 | 西北农林科技大学 | Automatic observation system for chlorophyll fluorescence of multi-angle sunlight induced guide vane |
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Inventor after: Liu Zhigang Inventor after: Chen Ji Inventor after: Yang Peiqi Inventor after: Wang Qingshan Inventor after: Zhang Weiwei Inventor after: Ni Zhuoya Inventor after: Zhou Xijia Inventor before: Liu Zhigang Inventor before: Chen Ji Inventor before: Yang Peiqi Inventor before: Wang Qingshan Inventor before: Zhang Weiwei Inventor before: Ni Zhuoya Inventor before: Zhou Xi |
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Free format text: CORRECT: INVENTOR; FROM: LIU ZHIGANG CHEN JI YANG PEIQI WANG QINGSHAN ZHANG WEIWEI NI ZHUOYA ZHOU XI TO: LIU ZHIGANG CHEN JI YANG PEIQI WANG QINGSHAN ZHANG WEIWEI NI ZHUOYA ZHOU XIJIA |
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