CN104007094A - Blade active and inactive chlorophyll fluorescence long-time-series cooperative observation system - Google Patents

Blade active and inactive chlorophyll fluorescence long-time-series cooperative observation system Download PDF

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Publication number
CN104007094A
CN104007094A CN201410220579.2A CN201410220579A CN104007094A CN 104007094 A CN104007094 A CN 104007094A CN 201410220579 A CN201410220579 A CN 201410220579A CN 104007094 A CN104007094 A CN 104007094A
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collecting device
constant temperature
frame
temperature oven
simultaneous observation
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CN104007094B (en
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刘志刚
陈绩
杨沛琦
王庆山
张葳葳
倪卓娅
周西
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Beijing Normal University
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Beijing Normal University
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Abstract

The invention discloses a blade active and inactive chlorophyll fluorescence long-time-series cooperative observation system. The system consists of a synchronous observation frame, a spectrum collecting device, an active fluorescence collecting device, a photosynthetically active radiation collecting device and a host machine, wherein the spectrum collecting device, the active fluorescence collecting device and the photosynthetically active radiation collecting device are installed on the synchronous observation frame; the spectrum collecting device, the active fluorescence collecting device, the photosynthetically active radiation collecting device and the synchronous observation frame are connected with the host machine by data lines, and through the host machine, spectra, fluorescence kinetic parameters and photosynthetically active radiation data are collected and a motor on the synchronous observation frame is controlled. The blade active and inactive chlorophyll fluorescence long-time-series cooperative observation system is scientific in concept, simple in structure, convenient to carry and high in stability, effectively guarantees the consistency of measurement objects and a measurement environment during active and inactive fluorescence measurement, and can develop cooperative observation of long time series.

Description

The collaborative recording geometry of the moving long-time sequence of chlorophyll fluorescence of a kind of blade main quilt
Technical field
The present invention relates to the collaborative recording geometry of the moving long-time sequence of chlorophyll fluorescence of a kind of blade main quilt, it is the integrated system of studying vegetation spectrum and vegetation physiological relation in geography, can carry out passive sunlight-induced chlorophyll fluorescence (Solar-induced chlorophyll fluorescence to blade, and the initiatively collaborative observation of long-time sequence of Change of Chlorophyll Fluorescence Kinetics Parameters SIF), be used for setting up passive fluorescence and active Change of Chlorophyll Fluorescence Kinetics Parameters and the physiological relation of vegetation, belong to physical geography systems technology field.
Background technology
Photosynthesis is the most key physiology course of vegetation, not only affects the material recycle such as carbon, water, and can fast, directly reflect the growth conditions of plant and be subject to stress state.Tradition remote sensing is by biophysical parameters and the biochemical parameters of the electromagnetic wave information inverting vegetation of vegetation reflection, radiation and scattering, as leaf area index, chlorophyll content etc., but also lack at present the remote sensing of effectively surveying plant photosynthesis information and being subject to stress state.Vegetation chlorophyll fluorescence is because closely related with plant photosynthesis, be considered to after deliberation " probe " of plant physiology state, closely bound up with the residing external environment of vegetation growth, the stress state that chlorophyll fluorescence is applied to detection vegetation has very large potentiality.Initiatively fluorescence measurement is the Change of Chlorophyll Fluorescence Kinetics Parameters that obtains vegetation leaf scale by active transponder pulse, has very important reference significance for the physiological Study of vegetation.SIF extracts fluorescence information by algorithm from sun incident light spectrum and the apparent reflectance spectrum of target, can obtain by remote sensing technique, can realize the physiological status observation of large area earth's surface vegetation.The SIF in low latitude, the whole world is all studied at present, but whether the SIF extracting from spectrum is the Correct of the true emitting fluorescence of vegetation, and can SIF correctly reflect the physiological status of vegetation, also needs further verify and determine.The precision of many kinds of extracting method of SIF and validity also need to analyze in addition.Therefore need a kind of system that can measure the moving fluorescence of main quilt simultaneously, this system should guarantee that measured target and the environment of the moving fluorescence measurement of main quilt keep consistency, and can facilitate, stably carry out long time-ordered measurement.We overlap light-duty, the automatic simultaneous observation system that the collaborative recording geometry of the moving long-time sequences of chlorophyll fluorescence of blade main quilts is developed for above these problems just.This cover system comprises spectra collection equipment, initiatively fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame and main control system equipment five parts form, can stably carry out the moving fluorescence simultaneous observation of main quilt of long-time sequence to blade target, in order to study passive fluorescence and active Change of Chlorophyll Fluorescence Kinetics Parameters and the physiological relation of vegetation.
Summary of the invention
(1) object:
The object of this invention is to provide the collaborative recording geometry of the moving long-time sequence of chlorophyll fluorescence of a kind of blade main quilt.It has ensured that external environment, blade interior physiology that the moving fluorescence of main quilt obtains are all consistent, thereby guarantees that both have strict corresponding relation.System has the convenience of good stability and operation, can realize the long-time sequence simultaneous observation of the moving fluorescence data of main quilt.
(2) technical scheme
The present invention is the collaborative recording geometry (Fig. 1) of the moving long-time sequence of chlorophyll fluorescence of a kind of blade main quilt.Whole system is made up of five parts by practical function: simultaneous observation frame (Fig. 2), spectra collection equipment (Fig. 3), active fluorescent collecting equipment (Fig. 4), photosynthetically active radiation collecting device (Fig. 5), main frame.Position annexation between them is: spectra collection equipment, active fluorescent collecting equipment, photosynthetically active radiation collecting device are arranged on simultaneous observation frame, spectra collection equipment, initiatively fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame all couple together by data line and main frame, realize the control of the motor on collection and the simultaneous observation frame of spectrum, Change of Chlorophyll Fluorescence Kinetics Parameters, photosynthetically active radiation data by main frame.The integration realization of above equipment the collaborative observation of the moving long-time sequence of fluorescence of main quilt.
Described simultaneous observation frame (Fig. 2) is assembled by stainless steel, four shaped steel compositions are cross as simultaneous observation frame base, simultaneous observation frame body frame is spliced by many shaped steel, between the object disposing platform of rectangle plate shape is installed, simultaneous observation frame body frame upper end connects observation part, can adjust observation Partial Height, observation unit is divided the sample platform that comprises the fixing montant of motor, reference plate pallet, HR4000 and PAM2500 optical fiber and rectangle plate shape.HR4000 spectrometer fibre-optical probe moves back and forth between above Observed Position and diffuse reflection reference Board position, has realized the collection (Fig. 6) of sun incident light spectrum and the apparent reflectance spectrum of target and provides spectrum and the stable environment of Change of Chlorophyll Fluorescence Kinetics Parameters synchronous acquisition (Fig. 7).
Described spectra collection equipment (Fig. 3) comprises constant temperature oven and built-in HR4000 spectrometer, diffuse reflection reference plate, can realize the measurement of sun incident light spectrum and the apparent reflectance spectrum of target, to extract SIF, constant temperature oven can be guaranteed the long steady operation of HR4000 spectrometer.This constant temperature oven is case structure, which is provided with HR4000 data line, 1HR4000 spectrometer optical fiber, constant temperature oven data line interface, constant temperature oven optical fiber interface, constant temperature oven power interface, constant temperature oven power supply changeover device and constant temperature oven power lead; This diffuse reflection reference plate is small circular plate, and it is lambert's body specific coatings of 99% that its surface is provided with reflectivity.
Described active fluorescent collecting equipment (Fig. 4) is PAM2500 luminoscope, can obtain the Change of Chlorophyll Fluorescence Kinetics Parameters of blade target.Which is provided with PAM2500 data line, PAM2500 optical fiber, PAM2500 data line interface, PAM2500 optical fiber interface and PAM2500 fibre-optical probe.
Described photosynthetically active radiation collecting device (Fig. 5) is PAR sensor and PAR main frame, can obtain current photosynthetically active radiation value.
Described main frame is a computer, HR4000 is installed and controls software, PAM2500 control software, PAR sensor control software, and acquisition method and the flow process of above data has been set.
Principle of the present invention and operating mode brief introduction are as follows: the present invention carries out PAM2500 luminoscope and HR4000 spectrometer integrated by simultaneous observation frame, provide the moving long-time sequence of chlorophyll fluorescence of a kind of blade main quilt collaborative recording geometry.System is by the operation of the motor on host computer control simultaneous observation frame, HR4000 spectrometer fibre-optical probe is switched between observed object and diffuse reflection reference plate, realize the collection of sun incident light spectrum and the apparent reflectance spectrum of observed object, in the time that spectra collection object is blade, gather initiatively fluorescence parameter and the apparent reflectance spectrum of blade and current photosynthetically active radiation value simultaneously.The automatic collection of spectrum, fluorescence, photosynthetically active radiation is set identical observed object is carried out to the moving chlorophyll fluorescence observation of main quilt of long-time sequence by main frame.
(3) advantage, effect
The collaborative recording geometry design of the moving long-time sequence of chlorophyll fluorescence of this blade main quilt science, simple in structure, be easy to carry.The consistance of measuring object and measurement environment when it has ensured the moving fluorescence measurement of main quilt preferably, system has stronger stability, can carry out the collaborative observation of long-time sequence.
Brief description of the drawings
Fig. 1 overall system schematic diagram
Fig. 2 simultaneous observation frame schematic diagram
Fig. 3 spectra collection equipment schematic diagram
Fig. 4 is fluorescent collecting equipment schematic diagram initiatively
Fig. 5 light and effectively radiation collecting device schematic diagram
Fig. 6 simultaneous observation frame motor operation schematic diagram
Fig. 7 simultaneous observation schematic diagram
In figure, symbol description is as follows:
1 simultaneous observation frame base; 2 simultaneous observation frame body frames; 3 object disposing platforms; 4 sample platforms; 5 reference plate pallets; 6HR4000 spectrometer optical fiber is fixed montant; 7PAM2500 optical fiber is fixed montant; 8 motors; 9 motor cable lines; 10 motor main frames; 11 motor supplying cells; 12 motor power-supply wires; 13 motor host data lines; 14 constant temperature ovens; 15HR4000 spectrometer; 16HR4000 data line; 17HR4000 spectrometer optical fiber; 18 constant temperature oven data line interfaces; 19 constant temperature oven optical fiber interfaces; 20 constant temperature oven power interfaces; 21 constant temperature oven power supply changeover devices; 22 constant temperature oven power leads; 23 diffuse reflection reference plates; 24PAM2500 luminoscope; 25PAM2500 data line; 26PAM2500 optical fiber; 27PAM2500 data line interface; 28PAM2500 optical fiber interface; 29PAR main frame; 30PAR sensor; 31PAR host data line; 32 main frames; 33HR4000 spectrometer fibre-optical probe; 34PAM2500 fibre-optical probe; 35 observed objects.
Embodiment
As shown in Fig. 1-Fig. 7, the collaborative recording geometry of the moving long-time sequence of chlorophyll fluorescence of a kind of blade main quilt of the present invention is made up of spectra collection equipment, active fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame, 32 5 parts of main frame.Position annexation between them is: spectra collection equipment, active fluorescent collecting equipment, photosynthetically active radiation collecting device are arranged on simultaneous observation frame, spectra collection equipment, active fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame all couple together by data line and main frame, realize the control of the motor on spectrum, Change of Chlorophyll Fluorescence Kinetics Parameters, PAR data acquisition and simultaneous observation frame by main frame 32.The integration realization of above equipment the collaborative observation of the moving long-time sequence of fluorescence of main quilt.
Described spectra collection equipment is HR4000 spectrometer 15 and annex thereof, and this spectrometer has 0.02nm spectral resolution, and spectral range covers 700-800nm, can extract the fluorescent emission peak value that is positioned at 760nm place.HR4000 spectrometer 15 is positioned in constant temperature oven 14, realizes signal transmission by constant temperature oven data line interface 18 and constant temperature oven optical fiber interface 19.During constant temperature oven 14 is measured HR4000 spectrometer 15, temperature constant is at 23 DEG C, and constant temperature oven 14 is powered by external power supply through constant temperature oven power lead 22 and constant temperature oven power supply changeover device 21.HR4000 spectrometer fibre-optical probe 33 is fixed on the fixing montant 6 of HR4000 spectrometer optical fiber on simultaneous observation frame, and diffuse reflection reference plate 23 is fixed in simultaneous observation frame reference plate pallet 5.Main frame 32 is controlled the operation of motor 8 on simultaneous observation frame by motor host data line 13, make HR4000 spectrometer fibre-optical probe 33 can complete the switching to observed object 35 and diffuse reflection reference plate 23, obtain respectively sun incident light spectrum and the reflectance spectrum of observed object, calculate sunlight-induced chlorophyll fluorescence by existing algorithm.This constant temperature oven 14 is case structures, which is provided with HR4000 data line 16, HR4000 spectrometer optical fiber 17, constant temperature oven data line interface 18, constant temperature oven optical fiber interface 19, constant temperature oven power interface 20, constant temperature oven power supply changeover device 21 and constant temperature oven power lead 22.
Described active fluorescent collecting equipment is PAM2500 luminoscope 24 and annex thereof, PAM2500 luminoscope fibre-optical probe 34 keeps relative position relation constant with observed object 35, PAM2500 luminoscope fibre-optical probe 34 obtains the active fluorescence signal of observed object 35, signal is transferred to main frame 32 through PAM2500 data line 25, and PAM2500 luminoscope 24 which is provided with PAM2500 data line 25, PAM2500 optical fiber 26, PAM2500 data line interface 27 and PAM2500 optical fiber interface 28; Its built-in power can complete the time-ordered measurement of 8 hours.
Described photosynthetically active radiation collecting device is made up of PAR sensor 30 and PAR main frame 29, is powered by No.5 cell group, and the PAR data of obtaining are stored in PAR main frame 29, can import main frame 32 by PAR host data line 31.
Described simultaneous observation frame is assembled by stainless steel material, four shaped steel compositions are cross as simultaneous observation frame base 1, simultaneous observation frame body frame 2 is spliced by many shaped steel, simultaneous observation frame body frame 2 middle parts are provided with the object disposing platform 3 of rectangle plate shape, can place pertinent instruments and equipment, simultaneous observation frame body frame 2 upper ends connect observation part, observation Partial Height can regulate, observation unit is divided and is comprised motor 8, reference plate pallet 5, HR4000 optical fiber is fixed montant 6, the sample platform 4 of the fixing montant 7 of PAM2500 optical fiber and rectangle plate shape, HR4000 optical fiber is fixed montant 6, the fixing montant 7 of PAM2500 optical fiber is for fixing HR4000 spectrometer fibre-optical probe 33, PAM2500 luminoscope fibre-optical probe 34, sample platform 4 is used for placing observed object 35, the interior placement diffuse reflection reference plate 23 of reference plate pallet 5, PAR sensor 30 is arranged on simultaneous observation frame top horizontal level.Motor main frame 10 is connected with motor 8 by motor cable line 9, can control motor 8 operations, also can be connected to main frame 32 by motor host data line 13, and the operation of motor 8 is set automatically.Motor 8 rotates so that HR4000 spectrometer fibre-optical probe 33 object of observations switch between diffuse reflection reference plate 23 and observed object 35.Motor 8 is stepper motor, is powered by motor supplying cell 11, can realize motor 8 and work long hours.
Described main frame 32 is a computing machine that HR4000 control software, PAM2500 control software, PAR sensor control software and Electric Machine Control software are installed, initial position, traffic direction, travelling speed, target location and 23 position time out and the cycles of operation of diffuse reflection reference plate of motor 8 are set by motor host data line 13, realize the automatic switchover (Fig. 6) of the fixing montant 6 of HR4000 optical fiber in target location and diffuse reflection reference plate 23 positions.By HR4000 data line 16, PAM2500 data line 25, PAR host data line 31, PAM2500 luminoscope 24 and the simultaneous observation cycle of HR4000 spectrometer 15 to observed object 35 are set respectively, and the data collection cycle of PAR sensor 30, realize spectrum, the initiatively collaborative observation of fluorescence parameter, PAR.

Claims (1)

1. the collaborative recording geometry of the moving long-time sequence of chlorophyll fluorescence of blade main quilt, is characterized in that: this system is made up of simultaneous observation frame, spectra collection equipment, active fluorescent collecting equipment, photosynthetically active radiation collecting device and five parts of main frame; Spectra collection equipment, active fluorescent collecting equipment, photosynthetically active radiation collecting device are arranged on simultaneous observation frame, spectra collection equipment, initiatively fluorescent collecting equipment, photosynthetically active radiation collecting device, simultaneous observation frame all couple together by data line and main frame, realize the control of the motor on collection and the simultaneous observation frame of spectrum, Change of Chlorophyll Fluorescence Kinetics Parameters, photosynthetically active radiation data by main frame;
Described simultaneous observation frame is to be assembled by stainless steel, four shaped steel compositions are cross as simultaneous observation frame base, simultaneous observation frame body frame is spliced by many shaped steel, between the object disposing platform of rectangle plate shape is installed, simultaneous observation frame body frame upper end connects observation part, it highly can be adjusted, and observation unit is divided the sample platform that comprises motor, reference plate pallet, the fixing montant of optical fiber and rectangle plate shape; Spectrometer fibre-optical probe moves back and forth between above Observed Position and diffuse reflection reference Board position, has realized the collection of sun incident light spectrum and the apparent reflectance spectrum of target, and the stable environment of spectrum and Change of Chlorophyll Fluorescence Kinetics Parameters synchronous acquisition is provided;
Described spectra collection equipment comprises constant temperature oven and built-in spectrometer, diffuse reflection reference plate, can realize the measurement of sun incident light spectrum and the apparent reflectance spectrum of target, and to extract SIF, constant temperature oven can be guaranteed the long steady operation of spectrometer; This constant temperature oven is case structure, which is provided with data line, spectrometer optical fiber, constant temperature oven data line interface, constant temperature oven optical fiber interface, constant temperature oven power interface, constant temperature oven power supply changeover device and constant temperature oven power lead; This diffuse reflection reference plate is small circular plate, and it is lambert's body specific coatings of 99% that its surface is provided with reflectivity;
Described active fluorescent collecting equipment is luminoscope, can obtain the Change of Chlorophyll Fluorescence Kinetics Parameters of blade target, which is provided with data line, optical fiber, data line interface, optical fiber interface and fibre-optical probe;
Described photosynthetically active radiation collecting device is PAR sensor and PAR main frame, can obtain current photosynthetically active radiation value;
Described main frame is a computer, HR4000 is installed and controls software, PAM2500 control software, PAR sensor control software, and acquisition method and the flow process of above data has been set.
CN201410220579.2A 2014-05-23 2014-05-23 A kind of blade master passive chlorophyll fluorescence long-term sequence synergistic observation system Expired - Fee Related CN104007094B (en)

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IT201900020174A1 (en) * 2019-10-31 2021-05-01 Consiglio Nazionale Ricerche CALIBRATION DEVICE FOR MEASURING THE FLUORESCENCE OF CHLOROPHYLL INDUCED BY SUNLIGHT

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Publication number Priority date Publication date Assignee Title
CN105486673A (en) * 2016-01-27 2016-04-13 北京师范大学 Chlorophyll fluorescence automation monitoring system
CN105548122A (en) * 2016-01-27 2016-05-04 北京师范大学 Chlorophyll fluorescent time-shared monitoring system
CN105486673B (en) * 2016-01-27 2020-03-10 北京师范大学 Chlorophyll fluorescence automatic monitoring system
CN105548122B (en) * 2016-01-27 2020-04-17 北京师范大学 Chlorophyll fluorescence time-sharing monitoring system
CN105842220A (en) * 2016-05-26 2016-08-10 伯格森(北京)科技有限公司 Vegetation fluorescence time sequence measuring system and method
CN109580558A (en) * 2017-09-28 2019-04-05 中国科学院地理科学与资源研究所 A kind of sunlight-induced vegetation fluorescence measuring systems suitable for different canopy layers height
CN113008372A (en) * 2021-03-08 2021-06-22 西北农林科技大学 Automatic observation system for chlorophyll fluorescence of multi-angle sunlight induced guide vane

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