CN105842220A - Vegetation fluorescence time sequence measuring system and method - Google Patents
Vegetation fluorescence time sequence measuring system and method Download PDFInfo
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- CN105842220A CN105842220A CN201610357433.1A CN201610357433A CN105842220A CN 105842220 A CN105842220 A CN 105842220A CN 201610357433 A CN201610357433 A CN 201610357433A CN 105842220 A CN105842220 A CN 105842220A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Abstract
The invention discloses a vegetation fluorescence time sequence measuring system and method. The automatic measuring system comprises a host computer part, a spectrograph part, a power supply part and a measuring bracket, wherein the power supply part is used for supplying power to the host computer part and the spectrograph part; a rotating motor is arranged on the measuring bracket; a spectrum fluorescence detector is arranged on a rotating rod connected with the rotating motor; a standard reference plate is further arranged on the measuring bracket; the host computer part is electrically connected with the spectrograph part for controlling data storage of the spectrograph part; the host computer part is electrically connected with the rotating motor to control the rotating motor to rotate on a horizontal plane; the spectrograph part is connected with the spectrum fluorescence detector through optical fiber; the spectrum fluorescence detector is used for acquiring spectrum data.
Description
Technical field
The invention belongs in agricultural technology field or earth remote sensing scientific domain based on remote sensing technique planting
The technology that the photosynthesis of quilt is observed, is specifically related to Vegetation canopy sunlight-induced chlorophyll
The automatic measurement system of fluorescence and measuring method.
Background technology
Remote sensing of vegetation is the electromagnetic wave information inverting vegetation reflected by vegetation, radiate and scatter at present
Biophysical parameters and biochemical parameters, such as leaf area index, chlorophyll content etc., photosynthetic
Effect is the physiology course that vegetation is crucial, not only affects the circulation of the material such as carbon, water, and can
Quickly, the stress state of plant is directly reflected.But also lack effective detection vegetation light at present
Close the remote sensing of effect information.
Sunlight-induced chlorophyll fluorescence (Solar-Induced Fluorecence, SIF) and vegetation light
Cooperation is with in close relations, and fluorescence detection is the chlorophyll fluorescence signal obtaining sunlight-induced, is suitable for
Observation lossless to vegetation large area under natural environment, is therefore the most potential remote sensing of one,
Obtain increasingly extensive attention.
Periodical " Remote Sensing Principles of plant sunlight-induced chlorophyll fluorescence and progress " 2012 11
Month volume 27 o. 11th also summarizes the extraction algorithm of SIF, Remote Sensing Model, sensor and
The latest developments of the field application such as detection and efficiency of light energy utilization estimation are coerced in early days in vegetation.
Current research shows, utilizes the high spectrum resolution remote sensing technique can be at the concealed wire wave band of sun incident light
(such as oxygen absorption band) extracts the sunlight-induced chlorophyll fluorescence (solar-induced of vegetation
Fluorescence, SIF).This makes it possible to remote sensing technique based on SIF Development of Novel, right
The photosynthesis of vegetation is directly observed, thus serve global change research due, agricultural the yield by estimation with
The key areas such as disaster alarm, carbon cycle and carbon transaction.SIF remote sensing is at the early-stage, in global range
Need to carry out substantial amounts of observation experiment.Measuring different from clutter reflections rate, the SIF of Vegetation canopy deposits
At obvious diurnal variation and season crack, generally require long-term observation.Additionally, SIF itself is the faintest,
Its Regulatory requirements observed is significantly higher than general clutter reflections rate measure, is therefore badly in need of automatically
Change measurement system, with continuous print, obtain data in high quality.There is presently no the commercialization of maturation
System can reach this requirement.
Summary of the invention
In order to overcome the above-mentioned deficiency of prior art, solve current business and experimental study urgently needs
The photosynthesis of vegetation is directly observed by remote sensing technique based on SIF, the invention provides one
Plant vegetation fluorescence time-ordered measurement system, based on the remote sensing principle sunlight-induced chlorophyll to vegetation
Fluorescence (solar-induced Fluorescence, SIF) detects, and its Core Feature is as the criterion
Synchronously measure into shining the descending spoke brightness of Vegetation canopy and the apparent spoke brightness that Vegetation canopy is up,
The vegetation fluorescence time-ordered measurement system that the present invention provides uses non-imaged spectrometer to carry out spectral measurement,
Wherein enter the descending spoke brightness according to Vegetation canopy to be obtained, on Vegetation canopy by observation standard reference plate
Row apparent spoke brightness by observation canopy obtain, and use SIF extraction algorithm to data at
Reason.
Concrete, the technical scheme is that
Vegetation fluorescence time-ordered measurement system, including main frame portion, spectrometer section, to main frame portion and spectrum
Instrument portion provides the power supply of power supply and measures support, and described measurement support is provided with electric rotating machine,
Connecting and be provided with spectrum fluorescent probe on the swingle of electric rotating machine, described measurement support also sets up
There is the canonical reference plate that light is reflected；Described main frame portion electrically connects described spectrometer section and controls
The data storage of spectrometer section, described main frame portion electrically connects described electric rotating machine and controls described electric rotating
Machine carries out the rotation of horizontal plane；Described spectrometer section connects described spectrum fluorescent probe by optical fiber,
Described spectrum fluorescent probe gathers spectroscopic data.
Preferably, the described vinyl chloride sheet that canonical reference plate is grey, face coat is calcium oxide.
Preferably, measure support and include base, elongated portion and cross bar, described base and described elongation
Portion is fixing to be connected, and described elongated portion is fixing with described cross bar to be connected.
Preferably, elongated portion adds gusseted bar with the junction of described cross bar.
Preferably, the base measuring support uses axle center symmetric mode non-fully by pole or flat board
Constitute.
Preferably, base bottom is provided with fixed part, fixing connection base and ground.
Preferably, pole being provided with slide rail, fixing seat can be located in the enterprising line slip of slide rail.
Preferably, the elongated portion height measuring support is 3.8m-4.5m, a length of 1.2m-1.8m of cross bar.
Preferably, elongated portion uses double-decker, and internal layer is the cylindric aluminium section bar of 4cm-6cm thickness
Making, outer layer is that the aluminium alloy of 0.5cm-1cm thickness is made, and outer layer and internal layer are carried out by long spiro nail
It coincide fixing.
A kind of vegetation fluorescence time-ordered measurement method, carries out one group according to vegetation fluorescence time-ordered measurement system
When sunlight-induced chlorophyll fluorescence is measured, specifically include following steps:
Step S1: control electric rotating machine rotating rotary rods by main frame portion, drives spectrum fluorescence detection
Device rotates, and makes spectrum fluorescent probe alignment criteria reference plate；
Step S2: system optimization observation time of integration above canonical reference plate；
Step S3: spectrum fluorescent probe records the spectroscopic data of canonical reference plate；
Step S4: control electric rotating machine rotating rotary rods by main frame portion, drives spectrum fluorescence detection
Device rotates, and makes spectrum fluorescent probe be directed at Vegetation canopy to be measured；
Step S5: system optimization observation time of integration above Vegetation canopy to be measured；
Step S6: spectrum fluorescent probe records Vegetation canopy spectroscopic data.
Preferably, observation the time of integration i.e. spectrum fluorescent probe stop exposure time, described from
Dynamic measurement system is optimized setting automatically according to illumination, temperature and/or humidity extraneous when measuring,
Vegetation fluorescence time-ordered measurement method uses SIF extraction algorithm.
Compared with prior art, the vegetation fluorescence time-ordered measurement system of present invention offer and measuring method,
Have the advantages that
1) present invention provides vegetation fluorescence time-ordered measurement system and measuring method can be carried out at scene automatically
Detection, by the detection of sunlight-induced chlorophyll fluorescence being obtained the chlorophyll fluorescence signal of sunlight-induced,
Observation lossless to vegetation large area under natural environment, thus serve global change research due, agricultural is estimated
The key areas such as product and disaster alarm, carbon cycle and carbon transaction.
2) present invention provides vegetation fluorescence time-ordered measurement system and measuring method can photosynthetic to vegetation
Effect is directly observed, and can meet business and research with continuous print, obtain data in high quality
Needs.
3) present invention provides vegetation fluorescence time-ordered measurement system and measuring method are rotated by motor
Can realize quickly cutting in entering the descending spoke brightness according to Vegetation canopy and up apparent spoke brightness measurement
Changing, reduce the calculating error that incident light stably brings, meanwhile, motor rotates and has the highest weighing
Renaturation and stability, improve the quality of data.
4) present invention provides vegetation fluorescence time-ordered measurement system and measuring method only use an optical fiber
With a spectrometer, both can reduce the uncertainty that multiple light path is brought, and it also avoid light path and cut
Change caused signal strength weakening.
5) this technical scheme is suitable for the field automatic Observation of long period, has the highest cost performance.
Accompanying drawing explanation
Fig. 1 is the ground incident irradiance degree of normal atmosphere；
Fig. 2 is fluorescence spectrum and reflectance spectrum exemplary plot；
Fig. 3 is the structural representation of vegetation fluorescence time-ordered measurement system of the present invention；
Fig. 4 is the front view measuring support of vegetation fluorescence time-ordered measurement system of the present invention；
Fig. 5 is the sectional view measuring support of vegetation fluorescence time-ordered measurement system of the present invention；
Fig. 6 is the Vegetation canopy list group measurement procedure signal of vegetation fluorescence time-ordered measurement system of the present invention
Figure；
Fig. 7 is the top view measuring support of vegetation fluorescence time-ordered measurement system of the present invention.
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below in conjunction with
Accompanying drawing in the embodiment of the present invention, carries out clear, complete to the technical scheme in the embodiment of the present invention
Ground describes, it is clear that described embodiment is a part of embodiment of the present invention rather than whole
Embodiment.Generally herein described in accompanying drawing and the assembly of the embodiment of the present invention that illustrates can be with respectively
Plant different configurations to arrange and design.Therefore, reality to the present invention provided in the accompanying drawings below
The detailed description executing example is not intended to limit the scope of claimed invention, but is merely representative of
The selected embodiment of the present invention.Based on the embodiment in the present invention, those of ordinary skill in the art exist
Do not make the every other embodiment obtained under creative work premise, broadly fall into the present invention and protect
The scope protected.
The vegetation fluorescence time-ordered measurement system of the present invention is based on the day photo-induction to vegetation of the remote sensing principle
Leading chlorophyll fluorescence (solar-induced Fluorescence, SIF) to detect, remote sensing is visited
Survey principle is as follows:
Assume that the transmitting of fluorescence and the reflection on atural object surface are all lamberts, then vegetation is wave band λ's
Apparent spoke brightness L (λ) is made up of the reflection of incident light and fluorescence two parts of Plant emission:
L (λ)=r (λ) × E (λ)/π+F (λ) (1)
In formula (1): F (λ) is the fluorescent value of λ wave band, r (λ) is to discharge nothing with fluorescence
The real reflectance (Actual reflectance) closed, E (λ) is the irradiation level inciding vegetation.
Research proves that SIF penetrates lower the faintest at natural light, be only equivalent to vegetation absorb luminous energy 0.5%～
2%, it is difficult to accurately measure.Due to the air absorption to solar spectrum, arrive the solar spectrum on earth's surface
In have many waveband widths to be 0.1nm～10nm concealed wire, i.e. fraunhofer absorb concealed wire.
Fig. 1 is the ground incident irradiance degree of normal atmosphere, in ruddiness and near infrared band scope, has three
More significant concealed wire is i.e.: hydrogen absorbs the H α concealed wire formed at 656nm；In air, oxygen molecule absorbs
O2-A concealed wire that 760 and 687nm are formed about and O2-B concealed wire.
Fig. 2 is fluorescence spectrum and reflectance spectrum exemplary plot, absorbs concealed wire wave band at fraunhofer, plants
The reflection light of quilt is the faintest, and fluorescence highlights, and the apparent reflectance showing as vegetation is anti-more than true
Penetrating rate, wherein farther out, O2-A and O2-B concealed wire is green at leaf at H α concealed wire distance chlorophyll fluorescence peak
In element fluorescence peak, but the depth and width of O2-A concealed wire are both greater than O2-B concealed wire, and therefore O2-A is dark
Line is considered optimal remote sensing fluorescence detection wave band.
The technical scheme that the vegetation fluorescence time-ordered measurement system of the present invention provides is namely based on current planting
By canopy SIF measuring principle, its Core Feature is as the criterion and synchronously measures into shining the descending of Vegetation canopy
Spoke brightness and the up apparent spoke brightness of Vegetation canopy, the vegetation fluorescence time-ordered measurement that the present invention provides
System uses non-imaged spectrometer to carry out spectral measurement, wherein enters the descending spoke brightness according to Vegetation canopy
Being obtained by observation standard reference plate, the up apparent spoke brightness of Vegetation canopy is obtained by observation canopy
Taking, data acquisition between the two is rotated by motor and realizes switching.
The present invention is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art
Can implement according to this with reference to specification word.
Embodiment 1
Fig. 3 is the structural representation of vegetation fluorescence time-ordered measurement system of the present invention, including main frame portion 100,
Spectrometer section 200, the power supply 300 that main frame portion 100 and spectrometer section 200 are provided power supply and survey
Amount support 400, measures and is provided with electric rotating machine 101 on support 400, connect the rotation of electric rotating machine 101
It is provided with spectrum fluorescent probe 201 on bull stick, measures support 400 and be additionally provided with canonical reference plate 401,
Main frame portion 100 electrically connects spectrometer section 200 and electric rotating machine 101, controls electric rotating machine 101 and carries out water
The rotation of plane also completes the storage of the data to spectrometer section 200, and spectrometer section 200 is by optical fiber even
Connecing spectrum fluorescent probe 201, spectrum fluorescent probe 201 carries out vegetation spectrum number to Vegetation canopy
According to collection.
Wherein, canonical reference plate 401 is positioned at the lower section of swingle Plane of rotation, and concrete is arranged at light
Below the surfaces of revolution of spectrum fluorescent probe 201.
Fig. 4 is the front view measuring support 400 of embodiment of the present invention vegetation fluorescence time-ordered measurement system,
Measure support 400 and include base 410, elongated portion 420 and cross bar 430, ensure to survey during measuring
Measure the stability of support 400 and keep not shaking for systematic survey number under outdoor various interference
According to accuracy extremely important, the junction of base 410 and elongated portion 420 and elongation in the present embodiment
Portion 420 and the junction of cross bar 430 use strict fixing be connected, especially at elongated portion 420 and cross bar
The junction of 430 adds gusseted bar 440 makes cross bar 430 more stable, it is ensured that spectrum fluorescence
The positional stability of detector 201.
In the present embodiment, the base 410 measuring support 400 can be that the pole that quantity does not limits is constituted,
Can also be made up of the base plate of arbitrary shape, it is also possible to increase fixed part for 410 times at base and make further
Base 410 is fixed with supporters such as ground, and base 410 can be any can to make measurement in a word
The relative ground of support 400 keeps firm structure.
Further, base 410 uses axle center symmetric form non-fully, makes measurement support 400 phase
More firm for ground, especially ensure that and keep steady in the environment of there is wind in the external world when outdoor measurement
Gu；
Elongated portion 420 height measuring support 400 in the present embodiment is 3.8m-4.5m, and cross bar 430 is long
Degree is 1.2m-1.8m.
In order to strengthen measuring the steadiness of support 400, the base that the embodiment of the present invention provides further
410 and elongated portion 420 aluminium section bar that uses density qualities relatively large make, cross bar 430 uses quality
The relatively small light type aluminium section bar of density is made.
Fig. 5 is the sectional view measuring support 400 of the automatic measurement system of the embodiment of the present invention, elongation
Portion 420 uses double-decker, internal layer 421 to be that the cylindric aluminium section bar of 4cm-6cm thickness is made, outer layer
422 are made up of the aluminium alloy of 0.5cm-1cm thickness, and outer layer 422 is evenly arranged with in same horizontal plane
Screw slots 4221, can arrange long spiro nail 423 in screw slots 4221, corresponding internal layer 421 is cylindric
Being provided with screw hole on aluminium section bar, ectonexine structure carries out coincideing by long spiro nail 423 and fixes.
Fig. 6 is the Vegetation canopy list group measurement procedure schematic diagram of embodiment of the present invention automatic measurement system,
When the method for automatic measurement that the embodiment of the present invention provides carries out the measurement of one group of SIF, specifically include following
Step:
Step S1: control electric rotating machine 101 rotating rotary rods by main frame portion 100, drives spectrum glimmering
Photo-detector 201 rotates, and makes spectrum fluorescent probe 201 alignment criteria reference plate 401；
Step S2: system optimization observation time of integration above canonical reference plate 401；
Step S3: spectrum fluorescent probe 201 records the spectroscopic data of canonical reference plate 401；
Step S4: control electric rotating machine 101 rotating rotary rods by main frame portion 100, drives spectrum
Fluorescent probe 201 rotates, and makes spectrum fluorescent probe 201 be directed at Vegetation canopy to be measured；
Step S5: system optimization observation time of integration above Vegetation canopy to be measured；
Step S6: spectrum fluorescent probe 201 records Vegetation canopy spectroscopic data.
More than i.e. automatic measurement system carries out the measuring process of one group of SIF, and default can be according to survey
Amount needs to carry out the measurement of many groups SIF of special time, and step makes by main frame portion 100 later
Control electric rotating machine 101 rotating rotary rods, drive spectrum fluorescent probe 201 to rotate, make spectrum
Fluorescent probe 201 realigns canonical reference plate 401 and starts the measurement of new one group of data, repeats
Above step S1-step S6.
In step S2 and step S5, i.e. spectrum fluorescent probe 201 stopped and exposed the observation time of integration
The time of light, system is automatic according to measuring the situations such as the ambient light photograph of situation at that time, temperature and humidity
Arranging, the time of integration, method of adjustment was: when outdoor light is strong, and the spectroscopic data collected is more than
During certain numerical value, reduce acquisition time；When outdoor insufficient light, the spectroscopic data collected is less than
During certain numerical value, increase acquisition time.
Meanwhile, the observation time of integration optimizing spectrometer is the data in order to obtain high s/n ratio, makes
Measurement data is more accurate, casts aside external interference and obtains closer to real data.
Wherein, canonical reference plate 401 is the vinyl chloride sheet of grey, and face coat is calcium oxide, mark
The reflectance factor of quasi-reference plate 401 can reach 18%, and the acting as of canonical reference plate 401 realizes light
Fully reflective, the numerical value recorded can be deducted from spectral background as reflecting background.
Main frame portion 100 is held what the SIF of Vegetation canopy to be measured measured repeatedly by software control realization
OK, thus realize the automatic measurement system of the embodiment of the present invention and enter in outdoor under being not required to manual operation
The Continuous Observation of row SIF.
The automatic measurement system of the embodiment of the present invention is obtained by spectrum fluorescent probe 201 and organizes mark more
Quasi-reference plate 401 spectroscopic data and Vegetation canopy spectroscopic data to be measured, then extract according to SIF and calculate
Method obtains the sunlight-induced chlorophyll fluorescence data of Vegetation canopy to be measured, and the most conventional SIF extracts
Method is method based on spoke brightness measurement, and such method typically utilizes one in sun incidence brightness
The wave band of line and the apparent spoke brightness of one or more wave band outside concealed wire, based on certain hypothesis
Estimation SIF.
SIF extraction algorithm is divided into two big classes at present: method based on spoke brightness and side based on reflectivity
Method:
1) method based on spoke brightness
Such method utilizes a wave band (λ in) in fraunhofer line and one (or multiple)
The apparent spoke brightness of the wave band (λ out) outside fraunhofer line, based on certain it is assumed that estimation
The most light activated fluorescence filling extent to " fraunhofer well ", obtains fluorescence information, permissible
Abstract it is formulated as:
F=f (L (λ in), L (λ out-1), L (λ out-2) ..., L (λ out-n)) (2)
In formula (2), L (λ in) is the apparent spoke brightness of wave band, L (λ out in fraunhofer line
-i) it is the apparent spoke brightness of the i-th ∈ [1, n] wave band outside fraunhofer line, calculate function f and set up
Inside and outside fraunhofer line on the reflectivity of wave band and certain relevance assumption of fluorescence.
2) method based on reflectivity
Based on reflectivity obtain the algorithm of fluorescence information essentially by analysis of fluorescence to 650nm～
The impact of 800nm red edge regions reflectivity obtains fluorescence information, and obtain is a reaction fluorescence intensity
Reflectivity index rather than a clear and definite physical quantity.Fluorescence index based on reflectivity is permissible
It is divided three classes: luminance factor value index number (Reflectance Ratios), reflectivity first derivative refers to
Number (Derivatives) and filling index (infilling index).
What luminance factor value index number utilized is one is affected strong wave band (680nm or 740nm) by fluorescence
With a reflectivity being affected weak wave band by fluorescence.Removed with anti-with the former ratio by the latter
The spectral information that rate of penetrating is relevant, to obtain fluorescence information, conventional index have r690/r600 and
R740/r800 etc..
Reflectivity derivative index, as luminance factor value index number, is also a kind of method for normalizing.But
Reflectivity derivative index uses reflectivity first derivative rather than reflectivity itself calculates.
Embodiment 2
The difference of the present embodiment 2 and embodiment 1 is to further define vegetation fluorescence time-ordered measurement system
The measurement support 400 of system.
The elongated portion height measuring support 400 in the present embodiment is 4.116m, a length of 1.5m of cross bar.
Figure is the top view measuring support of embodiment of the present invention vegetation fluorescence time-ordered measurement system.
The base 410 measuring support 400 uses axle center symmetric mode non-fully, four supports divide
Wei support 411, support 412, support 413 and support 414 not constitute, four supports are with elongated portion 420
It is fixedly connected centered by intersection point, and forms both sides with the line of symmetry A of the bearing of trend of cross bar 430
Symmetric shape；
Concrete, support 411 is equal with support 412 length and symmetrical according to line of symmetry A both sides, support
413 and support 414 length is equal and symmetrical according to line of symmetry A both sides, the length of support 411 more than
The length of frame 413, the length of support 412 is more than the length of support 414.
Embodiment 3
The difference of the present embodiment 3 and embodiment 1 is to further define vegetation fluorescence time-ordered measurement system
The measurement support 400 of system.
When the base 410 measuring support 400 is made up of pole, pole is provided with slide rail, fixing
Seat can be located in the enterprising line slip of slide rail, thus according to different outdoor test environment adjustable frames
410 with the fixing point of the supporter such as ground, make vegetation fluorescence time-ordered measurement system be applicable to different
The place of observation condition.
Embodiment 4
The present embodiment 4 is to further define the survey of vegetation fluorescence volume system with the difference of embodiment 1
Amount support 400.
The elongated portion 420 measuring support 400 uses double-decker, internal layer 421 to be by the circle of 5cm thickness
Tubular aluminium section bar is made, and outer layer 422 is made up of the aluminium alloy that 0.6cm is thick.
These are only present pre-ferred embodiments, be not used to limit to the present invention, all the present invention's
Amendment, equivalent and the improvement etc. made within spirit and principle, are required to be included in the present invention
Protection domain within.
Claims (10)
1. vegetation fluorescence time-ordered measurement system, it is characterised in that include main frame portion (100), spectrum
Instrument portion (200), main frame portion (100) and spectrometer section (200) are provided the power supply (300) of power supply
And measure support (400), described measurement support (400) is provided with electric rotating machine (101),
Connect and be provided with spectrum fluorescent probe (201) on the swingle of electric rotating machine (101), described
Measure support (400) and be additionally provided with the canonical reference plate (401) that light is reflected；Described
Main frame portion (100) electrically connects described spectrometer section (200) and controls the data of spectrometer section (200)
Storage, described main frame portion (100) electrically connects described electric rotating machine (101) and controls described electric rotating
Machine (101) carries out the rotation of horizontal plane；Described spectrometer section (200) connects described by optical fiber
Spectrum fluorescent probe (201), described spectrum fluorescent probe (201) gathers spectroscopic data.
Vegetation fluorescence time-ordered measurement system the most according to claim 1, it is characterised in that institute
The canonical reference plate (401) stated is the vinyl chloride sheet of grey, and face coat is calcium oxide.
Vegetation fluorescence time-ordered measurement system the most according to claim 1, it is characterised in that institute
The measurement support (400) stated includes base (410), elongated portion (420) and cross bar (430),
Described base (410) and described elongated portion (420) are fixing to be connected, described elongated portion (420) and
Described cross bar (430) is fixing to be connected.
Vegetation fluorescence time-ordered measurement system the most according to claim 3, it is characterised in that institute
The elongated portion (420) stated adds gusseted bar (440) with the junction of described cross bar (430).
Vegetation fluorescence time-ordered measurement system the most according to claim 3, it is characterised in that institute
The canonical reference plate (401) stated is arranged at below the surfaces of revolution of spectrum fluorescent probe (201).
Vegetation fluorescence time-ordered measurement system the most according to claim 3, it is characterised in that institute
Being provided with slide rail on the base (410) stated, by slide rail connecting portion, fixing seat can be located in
The enterprising line slip of slide rail.
Vegetation fluorescence time-ordered measurement system the most according to claim 3, it is characterised in that institute
Elongated portion (420) height of the measurement support (400) stated is 3.8m-4.5m, cross bar (430)
A length of 1.2m-1.8m.
Vegetation fluorescence time-ordered measurement system the most according to claim 3, it is characterised in that institute
The elongated portion (420) stated uses double-decker, and internal layer (421) is the cylindrical shape of 4cm-6cm thickness
Aluminium section bar is made, and outer layer (422) is that the aluminium alloy of 0.5cm-1cm thickness is made, outer layer (422)
Stake-fastening is carried out by long spiro nail (423) with internal layer (421).
9. a vegetation fluorescence time-ordered measurement method, it is characterised in that described measuring method is carried out
When one group of sunlight-induced chlorophyll fluorescence is measured, specifically include following steps:
Step S1: control electric rotating machine (101) rotating rotary rods, band by main frame portion (100)
Dynamic spectrum fluorescent probe (201) rotates, and makes spectrum fluorescent probe (201) alignment criteria join
Examine plate；
Step S2: system optimization observation time of integration above canonical reference plate；
Step S3: spectrum fluorescent probe (201) records the spectroscopic data of canonical reference plate；
Step S4: control electric rotating machine (101) rotating rotary rods, band by main frame portion (100)
Dynamic spectrum fluorescent probe (201) rotates, and makes spectrum fluorescent probe (201) be directed to be measured planting
By canopy；
Step S5: system optimization observation time of integration above accurate Vegetation canopy to be measured；
Step S6: spectrum fluorescent probe (201) records Vegetation canopy spectroscopic data.
Vegetation fluorescence time-ordered measurement method the most according to claim 9, it is characterised in that institute
The observation i.e. spectrum fluorescent probe (201) time of integration stated stops the time of exposure, described vegetation
Fluorescence time-ordered measurement method is optimized automatically according to illumination, temperature and/or humidity extraneous when measuring
Arrange；Described vegetation fluorescence time-ordered measurement method uses SIF extraction algorithm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108693154A (en) * | 2018-04-25 | 2018-10-23 | 南京大学 | A kind of method of multi-angle observation exact inversion vegetation negative and positive leaf sunlight-induced chlorophyll fluorescence |
| IT201900020174A1 (en) * | 2019-10-31 | 2021-05-01 | Consiglio Nazionale Ricerche | CALIBRATION DEVICE FOR MEASURING THE FLUORESCENCE OF CHLOROPHYLL INDUCED BY SUNLIGHT |
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