CN105784592A - Apparatus for multi-angle in situ spectrum detection of activity of peroxidase in tomato leaves, and method thereof - Google Patents
Apparatus for multi-angle in situ spectrum detection of activity of peroxidase in tomato leaves, and method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of spectrum detection, and especially relates to a spectrum apparatus for detecting the activity and the content of peroxidase in tomato leaves, in the tomato planting field, and a method thereof. The apparatus comprises a spectrum probe, a light source, an omnibearing gyroscope and a light insulation device, and the omnibearing gyroscope comprises an arc suspension plate, an annular track plate, electric driving rollers and supporting legs. The method comprises the following steps: 1, preparing the apparatus; 2, carrying out spectrum blank correction; 3, carrying out automatic spectral angle adjustment sampling on the leaves; 4, carrying out multi-point sampling on the spectrum of the leaves; and 5, calculating physical and chemical values. The apparatus is arranged in the tomato planting field to obtain the spectrum data of the activity of the peroxidase in the tomato leaves of living plants; the apparatus can measure the tomato leaves in an omnibearing multi-angle manner; and the electric driving roller automatically rotates, and a camera cooperates to observe and monitor, so the detection efficiency is high.
Description
Technical field
The present invention relates to technical field of spectral detection, particularly for the spectral device of tomato planting Site Detection tomato leaf peroxidase activity.
Background technology
Tamato fruit contains the various nutrient elements such as substantial amounts of saccharide, protein, aminoacid, vitamin and inorganic salts.So Fructus Lycopersici esculenti is welcome by common people in China very much, annual yield is very big.In tomato plant strain growth process; the free radical that resisting abiotic stress can be produced by the peroxidase activity in blade; protection is provided for plant; monitoring tomato growth Process of Peroxide enzymatic activity index; analyze the reason causing peroxidase activity index to change; there is provided foundation for tomato planting decision-making, provide scientific basis to the reasonable water and fertilizer management of tomato planting.
Tomato leaf Peroxidase Activity Determination is still mainly chemical assay and measures.Adopt chemical assay time and effort consuming, it is necessary to more human cost, analysis process has more mechanical manipulation, and is generally not suitable for great amount of samples is analyzed；Chemical analysis needs to use chemical reagent, to environment, certainly will take into account the accuracy of determination data, simple operation and environment friendly and pollution-free characteristic, utilizes spectroscopy technology to substitute conventional chemical analysis method and has obtained a degree of application.
As shown in Figure 1, because tomato leaf growthform is different, light source projects angle and spectroscopic probe head incident angle are required significantly high by in-site measurement, the method of existing tomato leaf peroxidase spectral detection adopts harvesting young leaves mostly, the fresh-keeping rotten accuracy to affect peroxidase spectral measurement of blade that prevents of pack trash ice is high to measuring peroxidase activity requirement of real-time.
Summary of the invention
For the problems referred to above, it is an object of the invention to provide suitable in Site Detection, measure the tomato leaf peroxidase activity apparatus and method of multi-angle In situ spectroscopic detection accurately.
For reaching above-mentioned purpose, the technical solution used in the present invention is: multi-angle In situ spectroscopic detection tomato leaf peroxidase activity device, include spectroscopic probe head, light source, it is different in that, also include all-directional rotation instrument and every electro-optical device, all-directional rotation instrument is by circular arc suspension plate, ring ring plate, electricity drives roller, supporting leg forms, circular arc suspension plate arc uniform arranges row's installing hole, light source and spectroscopic probe head select one of them installing hole to be connected in circular arc suspension plate according to crevice projection angle and incident angle respectively, circular arc suspension plate two head end is equipped with electricity and drives roller, ring ring plate is provided with the groove of opening upwards, electricity drives roller and embeds in groove along impression orbit traveling, ring ring plate bottom surface is connected to passive supporting leg；During detection, Fructus Lycopersici esculenti strain alive is in ring ring plate center, is entered wherein together with tested Fructus Lycopersici esculenti strain cover alive by all-directional rotation instrument every electro-optical device.
Including light shade cover, support it is preferred that described every electro-optical device, light shade cover is made by every luminescent material, and top has the cable of string holes, spectroscopic probe head and light source and optical fiber cable passes from string holes, and string holes place is provided with sealing-plug.
It is preferred that described circular arc suspension plate is also associated with photographic head.
Being made up of it is preferred that described electricity drives roller roller and motor, stepper motor output shaft is connected with roller wheel shaft.
It is preferred that be provided with the adjustable bolt regulating Circular Plate terrain clearance between described supporting leg and Circular Plate.
It is preferred that described circular arc suspension plate semicircular in shape, and it is marked with the graduation mark representing suspension plate arc angle at sidewall.
It is preferred that the installing hole number of described circular arc suspension plate is 18, adjacent holes angle is 10 °.
Also including electric block it is preferred that described every electro-optical device, light shade cover top is provided with suspension ring, and the suspension hook of electric block coordinates with suspension ring, and electric block is suspended on support.
It is preferred that described support is three-legged support.
The method of multi-angle In situ spectroscopic detection tomato leaf peroxidase: comprise the steps:
A () equipment prepares: erection bracket, lifting light shade cover, erection all-directional rotation instrument level leveling, Fructus Lycopersici esculenti strain alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively through installing hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover；
B () spectrum blank is corrected: blank placement concordant with Fructus Lycopersici esculenti strain blade height alive, carries out spectrum blank rectification；
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller and starts, circular arc suspension plate is driven to turn over certain angle together with spectroscopic probe head and light source, and coordinate adjustment ring ring plate height, consistent with the light path ensureing test point by photographic head observation monitoring, angle of incidence and angle of reflection are 90 °；Light source sends detection light to first test point, the wavelength utilizing spectroscopic probe head acquisition testing point place is 589nm, 750nm, 890nm, 1080nm, 1400nm, 1682nm, tomato leaf corresponding to 1982nm, 2240nm and 2300nm reflectance；
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other test point；
E () physics and chemistry value calculates: three point reflection rates of gained are inputted control unit respectively, and control unit is according to multiple linear regression equations:
Y=20.368X1+18.875X2+32.698X3-26.887X4+258.645X5-689.23X6+225.780
X7+258.664X8-398.335X9+102.89, calculates the peroxidase activity obtaining tomato leaf；In formula, Y is peroxidase activity in tomato leaf；The reflectance of the strain blade alive of the Fructus Lycopersici esculenti corresponding to X1 ~ X9 respectively wavelength 589nm, 750nm, 890nm, 1080nm, 1400nm, 1682nm, 1982nm, 2240nm and 2300nm, three test points obtain three peroxidase activity values, Y1, Y2 and Y3, Y1, Y2 and Y3 summation is made even and is in tomato leaf peroxidase activity Y.
Beneficial effects of the present invention has:
(1) all-directional rotation instrument is at tomato planting erected on site, can obtain the spectroscopic data of strain tomato leaf peroxidase alive, it is to avoid because of the in vitro rear tomato leaf inaccurate problem of peroxidase activity data.
(2) circular arc suspension plate is adopted to rotate at ring ring plate level, and select different suspension angles, and tomato leaf can be measured in all-dimensional multi-angle ground, can according to the natural growthform of different tomato leafs, change spectrum crevice projection angle and incident angle easily, obtain and preferably measure angle.
(3) adjustable bolt regulates Circular Plate terrain clearance, can according to different plant growth height, and spectral preservation probe and crop are apart from relative constancy, to obtain preferably spectroscopic data.
(4) use the light shade cover made every luminescent material by test instrunment together with tested crop cover into, stop the external environment impact on spectroscopic data, it is more accurate to measure.
(5) do not lifting by crane in electro-optical device situation, electricity drives roller and automatically drives circular arc suspension plate to rotate at ring ring plate level, photographic head coordinates the observation monitoring light path to ensure test point consistent simultaneously, solution drives roller without electricity and photographic head must repeatedly lift by crane every electro-optical device, the problem that detection efficiency is low.
(6) whole device is simple and practical, with low cost, it is easy to promote.
Accompanying drawing explanation
Fig. 1 is tomato leaf schematic diagram；
Fig. 2 is all-directional rotation instrument structural representation in the embodiment of the present invention；
Fig. 3 is every electro-optical device structural representation in the embodiment of the present invention；
Fig. 4 implements in the embodiment of the present invention to measure tomato leaf peroxidase activity result；
In figure, labelling indicates: 1 photographic head, 2 circular arc suspension plate, 3 spectroscopic probe head, 4 grooves, 5 ring ring plates, 6 electricity drive roller, 7 adjustable bolts, 8 supporting legs, 9 light sources, 10 supports, 11 string holes, 12 light shade covers, 13-electric block, 14 light source connecting seats, 15-photographic head connects seat, 16 spectroscopic probe head connect seat, the strain alive of 17-Fructus Lycopersici esculenti.
Detailed description of the invention
In order to be more fully understood that the present invention, below in conjunction with the drawings and specific embodiments, technical scheme is described further, referring to Fig. 2, Fig. 3 and Fig. 4:
Tomato leaf peroxidase activity device is detected by multi-angle In situ spectroscopic of the invention process, Main Function is to facilitate erected on site, and different natural growthform tomato leaf can be suitable for, so adopting all-directional rotation instrument to hang spectroscopic probe head 3 and light source 9, to change spectrum crevice projection angle and incident angle rapidly, obtain and preferably measure angle.Stopping the external environment impact on spectroscopic data every electro-optical device, it is more accurate to measure.
The structure that all-directional rotation instrument is concrete: include circular arc suspension plate 2, ring ring plate 5, electricity drive roller 6, supporting leg 8, circular arc suspension plate 2 arc uniform arranges row's installing hole, spectroscopic probe head 3 and light source 9 select one of them installing hole respectively according to crevice projection angle and incident angle, connect seat 16 respectively through light source connecting seat 14 and spectroscopic probe head and are connected in circular arc suspension plate 2.Measuring angle preferably to be able to be quickly found, in the present embodiment, installing hole adds up to 18, and every adjacent holes angle is 10 °.In order to record matching measurement result, circular arc suspension plate 2 sidewall in semicircular in shape is marked with the graduation mark representing suspension plate arc angle.2 liang of head ends of circular arc suspension plate are equipped with electricity and drive roller 6, ring ring plate 5 is hollow annular structure, which is provided with the groove 4 of opening upwards, electricity drives roller 6 and embeds in groove 4 and advance along its track, circular arc suspension plate 2 is promoted to coexist ring ring plate 5 Plane Rotation together with spectroscopic probe head 3 one, pass through the installing hole adjustment light source 9 of circular arc suspension plate 2 simultaneously and angle of incidence and the angle of reflection of spectroscopic probe head 3 is 90 °, reach the purpose that all-dimensional multi-angle is measured.
Ring ring plate 5 bottom surface is connected with passive supporting leg 8, in order to according to different plant growth height, spectral preservation probe 3 and crop distance relative constancy, to obtain preferably spectroscopic data.The present embodiment is provided with the adjustable bolt 7 regulating ring ring plate 5 terrain clearance between supporting leg 8 and ring ring plate 5.
Stopping external environmental light every electro-optical device, during detection, Fructus Lycopersici esculenti strain 17 alive is in ring ring plate 5 center, and all-directional rotation instrument is covered into wherein by light shade cover 12 together with the strain 17 alive of tested Fructus Lycopersici esculenti.Described being made up of light shade cover 12, support 10 every electro-optical device, light shade cover 12 is made by every luminescent material, and top has string holes 11, the cable of spectroscopic probe head 3 and light source 9 and data wire and passes from string holes 11, and string holes 11 place is provided with sealing-plug.Also including electric block 13 every electro-optical device, light shade cover 12 top is provided with suspension ring, and the suspension hook of electric block 13 coordinates with suspension ring, and electric block 13 hangs over the mount 10.Described support 10 is three-legged support 10.After light shade cover 12 cover is upper, in order to understand inner case better in real time, the present embodiment connects seat 15 by photographic head in circular arc suspension plate 2 photographic head 1 is installed.
During embodiment of the present invention work, the method for detection tomato leaf peroxidase activity, comprise the steps:
A () equipment prepares: erection bracket 10, lifting light shade cover 12, erection all-directional rotation instrument level leveling, Fructus Lycopersici esculenti strain 17 alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively through installing hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover；
B () spectrum blank is corrected: blank placement concordant with Fructus Lycopersici esculenti strain 17 blade height alive, carries out spectrum blank rectification；
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller 6 and starts, circular arc suspension plate 2 is driven to turn over certain angle together with spectroscopic probe head 3 and light source 9, and coordinate adjustment ring ring plate 5 height, consistent with the light path ensureing test point by photographic head 1 observation monitoring, angle of incidence and angle of reflection are 90 °；Light source 9 sends detection light to first test point, the wavelength utilizing spectroscopic probe head 3 acquisition testing point place is 589nm, 750nm, 890nm, 1080nm, 1400nm, 1682nm, tomato leaf corresponding to 1982nm, 2240nm and 2300nm reflectance；
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other test point；
E () physics and chemistry value calculates: three point reflection rates of gained are inputted control unit respectively, and control unit is according to multiple linear regression equations:
Y=20.368X1+18.875X2+32.698X3-26.887X4+258.645X5-689.23X6+225.780
X7+258.664X8-398.335X9+102.89, calculates the peroxidase activity obtaining tomato leaf；
In formula, Y is peroxidase activity in tomato leaf；The reflectance of strain 17 blade alive of the Fructus Lycopersici esculenti corresponding to X1 ~ X9 respectively wavelength 589nm, 750nm, 890nm, 1080nm, 1400nm, 1682nm, 1982nm, 2240nm and 2300nm.Three test points obtain three peroxidase activity values, Y1, Y2 and Y3, and Y1, Y2 and Y3 summation being made even is in tomato leaf peroxidase activity Y.
Saying from microcosmic, the active state of molecule of composition tomato leaf is subject to the energy excitation of detection light, produces different energy level transitions, absorbs and release energy, and forms corresponding with molecule reflectance spectrum and absorption spectrum.Different chemical bonds, such as O-H, N-H, C-H etc., the rotation of chemical bond and stretching vibration, can absorb the light of specific wavelength, and excite generation characteristic peak in transition between energy level, may determine that the composition of material according to the intensity of characteristic peak and wave number.
The vibration information of the chemical bonds such as wavelength used and N-H, C-N, the N-H in the peroxidase of tomato leaf and related chemical constituents is relevant, and these information can reflect the power of peroxidase activity in pears tomato leaf.Such as 750nm and 890nm reflects methyl and the methylene information of tomato leaf peroxidase, and 1400nm reflects the amido information of tomato leaf peroxidase, and 1982nm reflects tomato leaf peroxidase group CONH2Information and primary amide information, 2240nm and 2300nm reflects tomato leaf peroxidase group CH2Information.Features described above wavelength is modeled analyzing by application arithmetic of linearity regression, sets up the peroxidase activity of tomato leaf and the linear equation of characteristic wavelength.
The selected characteristic wavelength of the present invention is only for the quick detection of the peroxidase activity of tomato leaf, if needing to measure other indexs, it is necessary to selected specific wavelength again, by multiple linear regression, sets up model, calculates and obtain corresponding desired value.
Method provided by the invention is utilized to set up the index of correlation of model in Table 1, wherein, when modeling set representations sets up model, the reflectance and the existing method that utilize tomato leaf are tested the tomato leaf peroxidase activity matching obtained and are obtained model equation, forecast set represents, it was predicted that time, after namely having set up model, according to model equation, the characteristic wavelength reflectance of tomato leaf spectrum is substituted into the calculated tomato leaf peroxidase activity of model equation.
Table 1
| Data set | Number of samples | The coefficient of determination | Root-mean-square error (U/g) |
| Modeling collection | 100 | 0.6202 | 54.39 |
| Forecast set | 40 | 0.6022 | 69.81 |
Embodiment 1 ~ 10
Utilizing apparatus and method provided by the invention that tomato leaf peroxidase activity is tested, test result is in Table 2.
Table 2
By table 2 data, it can be seen that the peroxidase activity utilizing the tomato leaf that the inventive method records is close with the data that the test of existing method obtains, and utilizes the present invention can quickly measure the peroxidase activity of tomato leaf, reliable results.
Claims (10)
1. multi-angle In situ spectroscopic detection tomato leaf peroxidase activity device, include spectroscopic probe head, light source, it is characterized in that, also include all-directional rotation instrument and every electro-optical device, all-directional rotation instrument is by circular arc suspension plate, ring ring plate, electricity drives roller, supporting leg forms, circular arc suspension plate arc uniform arranges row's installing hole, light source and spectroscopic probe head select one of them installing hole to be connected in circular arc suspension plate according to crevice projection angle and incident angle respectively, circular arc suspension plate two head end is equipped with electricity and drives roller, ring ring plate is provided with the groove of opening upwards, electricity drives roller and embeds in groove along impression orbit traveling, ring ring plate bottom surface is connected to passive supporting leg；During detection, Fructus Lycopersici esculenti strain alive is in ring ring plate center, is entered wherein together with tested Fructus Lycopersici esculenti strain cover alive by all-directional rotation instrument every electro-optical device.
2. multi-angle In situ spectroscopic according to claim 1 detection tomato leaf peroxidase activity device, it is characterized in that: described include light shade cover, support every electro-optical device, light shade cover is made by every luminescent material, top has string holes, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, and string holes place is provided with sealing-plug.
3. multi-angle In situ spectroscopic according to claim 1 detection tomato leaf peroxidase activity device, it is characterised in that: described circular arc suspension plate is also associated with photographic head.
4. multi-angle In situ spectroscopic according to claim 1 detection tomato leaf peroxidase activity device, it is characterised in that: described electricity drives roller and is made up of roller and motor, and stepper motor output shaft is connected with roller wheel shaft.
5. multi-angle In situ spectroscopic according to claim 1 detection tomato leaf peroxidase activity device, it is characterised in that: it is provided with the adjustable bolt regulating Circular Plate terrain clearance between described supporting leg and Circular Plate.
6. multi-angle In situ spectroscopic according to claim 1 detection tomato leaf peroxidase activity device, it is characterised in that: described circular arc suspension plate semicircular in shape, and the graduation mark representing suspension plate arc angle it is marked with at sidewall.
7. multi-angle In situ spectroscopic according to claim 1 detection tomato leaf peroxidase activity device, it is characterised in that: the installing hole number of described circular arc suspension plate is 18, and adjacent holes angle is 10 °.
8. multi-angle In situ spectroscopic according to claim 2 detection tomato leaf peroxidase activity device, it is characterized in that: described also include electric block every electro-optical device, light shade cover top is provided with suspension ring, and the suspension hook of electric block coordinates with suspension ring, and electric block is suspended on support.
9. multi-angle In situ spectroscopic according to claim 2 detection tomato leaf peroxidase activity device, it is characterised in that: described support is three-legged support.
10. the method for multi-angle In situ spectroscopic detection tomato leaf peroxidase activity, it is characterised in that: comprise the steps:
A () equipment prepares: erection bracket, lifting light shade cover, erection all-directional rotation instrument level leveling, Fructus Lycopersici esculenti strain alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively through installing hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover；
B () spectrum blank is corrected: blank placement concordant with Fructus Lycopersici esculenti strain blade height alive, carries out spectrum blank rectification；
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller and starts, circular arc suspension plate is driven to turn over certain angle together with spectroscopic probe head and light source, and coordinate adjustment ring ring plate height, consistent with the light path ensureing test point by photographic head observation monitoring, angle of incidence and angle of reflection are 90 °；Light source sends detection light to first test point, the wavelength utilizing spectroscopic probe head acquisition testing point place is 589nm, 750nm, 890nm, 1080nm, 1400nm, 1682nm, tomato leaf corresponding to 1982nm, 2240nm and 2300nm reflectance；
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other test point；
E () physics and chemistry value calculates: three point reflection rates of gained are inputted control unit respectively, and control unit is according to multiple linear regression equations:
Y=20.368X1+18.875X2+32.698X3-26.887X4+258.645X5-689.23X6+225.780
X7+258.664X8-398.335X9+102.89, calculates the peroxidase activity obtaining tomato leaf；
In formula, Y is peroxidase activity in tomato leaf；The reflectance of the strain blade alive of the Fructus Lycopersici esculenti corresponding to X1 ~ X9 respectively wavelength 589nm, 750nm, 890nm, 1080nm, 1400nm, 1682nm, 1982nm, 2240nm and 2300nm, three test points obtain three peroxidase activity values, Y1, Y2 and Y3, Y1, Y2 and Y3 summation is made even and is in tomato leaf peroxidase activity Y.
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| CN109596577A (en) * | 2018-11-12 | 2019-04-09 | 河南农业大学 | The monitoring method that the construction method and wide angle of wheat powdery mildew state of illness monitoring model adapt to |
| CN112014330A (en) * | 2020-07-30 | 2020-12-01 | 北京农业智能装备技术研究中心 | Crop near-earth spectrum data acquisition method and device |
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Application publication date: 20160720 |