CN105954207A - Method for detecting content of chlorophyll b in canopy of jujube tree - Google Patents

Method for detecting content of chlorophyll b in canopy of jujube tree Download PDF

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Publication number
CN105954207A
CN105954207A CN201610361783.5A CN201610361783A CN105954207A CN 105954207 A CN105954207 A CN 105954207A CN 201610361783 A CN201610361783 A CN 201610361783A CN 105954207 A CN105954207 A CN 105954207A
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chlorophyll
jujube tree
canopy
content
tree canopy
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彭杰
牛建龙
窦中江
陈兵
王琼
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Tarim University
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Tarim University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55Specular reflectivity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The invention discloses a method for detecting content of chlorophyll b in canopy of jujube tree. The method comprises the following steps of collecting the high spectrum information of the canopy of jujube tree in sunny, cloudless or less-cloud weather with wind force less than 3; performing multiplicative scatter correction on the high spectrum data, and performing wavelet denoising and smoothing; extracting the spectrum reflectance values of the canopy of jujube tree with to-be-measured content of chlorophyll b in the wavelengths of 470nm, 610nm, 645nm, 652nm, 663nm, 985nm and 1160nm; substituting the spectrum reflectance values into a detection model, and calculating, so as to obtain the content of chlorophyll b in the canopy of jujube tree. The method has the characteristics that the pollution is avoided, the operation is simple, convenient, quick and accurate, and the method is suitable for being popularized and applied.

Description

A kind of detection method of jujube tree canopy content of chlorophyll b
Technical field
The invention belongs to plant cultivation field of engineering technology, relate to the detection method of a kind of content of chlorophyll b, specifically, relate to And the detection method of a kind of jujube tree canopy content of chlorophyll b.
Background technology
Chlorophyll b is the requisite nutrient of jujube growth, and quick obtaining chlorophyll b information is monitoring growth and development of jujube trees And the premise of quality guarantee.The detection method of the jujube tree canopy content of chlorophyll b that prior art is total needs to prepare chemical reagent, surveys The chemistry tail liquid emissions pollution to environment and the injury to human body during Ding, and there is complex operation step, the detection time is long Defect.Currently available technology is badly in need of pollution-free, easy and simple to handle, the most accurate a kind of jujube tree canopy content of chlorophyll b Detection method.
Summary of the invention
In order to overcome defect present in prior art, the present invention proposes a kind of Fructus Jujubae pollution-free, easy and simple to handle, the most accurate The detection method of crown canopy content of chlorophyll b, the method achieves the quick and precisely detection of jujube tree canopy content of chlorophyll b, no Need to prepare any chemical reagent, it is to avoid the chemistry tail liquid emissions pollution to environment and the injury to human body during measuring, with Time also greatly simplify operating procedure, shorten the detection time, require to obtain in the large area Fructus Jujubae garden short time can be met in agricultural production Take the demand of canopy content of chlorophyll b data, provide quick obtaining for fertile precisely the using of jujube tree field management Determination of Chlorophyll b The means of dose foundation.
Its technical scheme is as follows:
The detection method of a kind of jujube tree canopy content of chlorophyll b, comprises the following steps:
(1) sunny, cloudless or a small amount of cloud layer, the wind-force weather less than 3 grades is selected to gather required mensuration content of chlorophyll b jujube tree hat The hyperspectral information of layer.
(2), after high-spectral data being carried out multiplicative scatter correction, use wavelet de-noising, then be smoothed.
(3) extract the 470 of content of chlorophyll b jujube tree canopy to be determined, 610,645,652,663,985,1160nm wavelength Spectral reflectance values.
(4) reflectance value of each wavelength is substituted into jujube tree canopy content of chlorophyll b detection model:
Y=0.53796-30.89546x1-44.00625x2+506.2439x3-735.3674x4+289.2066x5-4.507388x6+8.1820 25x7
Wherein, y is jujube tree canopy content of chlorophyll b, and unit is mg/g, x1、x2、x3、x4、x5、x6、x7It is respectively Fructus Jujubae Crown canopy 470,610,645,652,663,985, the reflectance value of 1160nm wave band.
Preferably, the hyperspectral information collection of the jujube tree canopy described in step (1), during collection probe must vertically downward, And canopy center need to be directed at, probe is away from 1 meter of height of canopy.
Preferably, the hyperspectral information collection of the jujube tree canopy described in step (1), its acquisition time is Beijing time 11: 00-15:00.
Preferably, described in step (2), smoothing processing method is Savitzky-Golay Smoothing, and smooth window is 7.
Preferably, 470 described in step (4), 610,645,652,663,985, the spectrum of 1160nm wavelength anti- Radiance rate value, the reflectance of 10 times surveyed by same canopy repetition averagely after and through multiplicative scatter correction, wavelet de-noising peace Value after sliding process.
The invention have the benefit that
Present invention achieves the quick and precisely detection of jujube tree canopy content of chlorophyll b, it is not necessary to prepare any chemical reagent, it is to avoid During measuring, the chemistry tail liquid emissions pollution to environment and the injury to human body, also greatly simplify operating procedure simultaneously, contracting The short detection time, the need requiring to obtain canopy content of chlorophyll b data in agricultural production in the large area Fructus Jujubae garden short time can be met Ask, provide the means of quick obtaining dose foundation for fertile precisely the using of jujube tree field management Determination of Chlorophyll b.The method has There is feature pollution-free, easy and simple to handle, the most accurate, be suitable for popularization and application.
Accompanying drawing explanation
Fig. 1 is the fitting degree of jujube tree canopy content of chlorophyll b detected value and actual value.
Detailed description of the invention
With detailed description of the invention, technical scheme is described in more detail below in conjunction with the accompanying drawings.
The detection method of a kind of jujube tree canopy content of chlorophyll b, comprises the following steps:
(1) measure the hyperspectral information of the jujube tree canopy of content of chlorophyll b to be detected, select ceiling unlimited or partly cloudy and wind-force to be less than The weather of 3 grades is carried out, and the concrete minute of this example is on August 21st, 2015, and hyperspectral information acquisition time is Beijing Time 13:00-15:00, hyperspectral information acquisition instrument is the portable atural object of FieldSpec 4 that ASD company of the U.S. produces Spectrogrph, when hyperspectral information gathers, probe vertical is downward, and distance 1 meter of height of canopy, 10 light of each canopy collection are set a song to music Line, the meansigma methods of 10 curves is the final reflectance value of this canopy.This example acquires the EO-1 hyperion of 54 jujube tree canopies altogether Information.
(2) after 54 curves of spectrum gathered being carried out multiplicative scatter correction, wavelet de-noising and smoothing processing.
(3) extract every curve 470,610,645,652,663,985, the spectral reflectance values of 1160nm wavelength.
(4) by every curve 470,610,645,652,663,985, the spectral reflectance values of 1160nm wavelength substitute into jujube tree Canopy content of chlorophyll b detection model:
Y=0.53796-30.89546x1-44.00625x2+506.2439x3-735.3674x4+289.2066x5-4.507388x6+8.182025x7
Wherein, y is jujube tree canopy content of chlorophyll b, and unit is mg/g, x1、x2、x3、x4、x5、x6、x7It is respectively Fructus Jujubae Crown canopy 470,610,645,652,663,985, the reflectance value of 1160nm wave band.
(5) table 1 be the jujube tree canopy content of chlorophyll b utilizing indoor chemical assay to obtain actual value with utilize above-mentioned jujube tree The statistical data of the detected value that canopy content of chlorophyll b detection model obtains, as can be seen from Table 1, actual value and detected value two The meansigma methods of person, maximum, minima are more close;Fig. 1 is the plan of jujube tree canopy content of chlorophyll b detected value and actual value Conjunction degree.The coefficient of determination (R between actual value and detected value2) reaching 0.54, root-mean-square error (RMSE) only has 0.124mg/g, Average relative error (MRE) only 13.04%, standard deviation reaches 1.52 with root-mean-square error ratio (RPD).To sum up, model is described There is the predictive ability of certain precision.This result shows, the method for the present invention can be with degree of precision, quickly detection jujube tree canopy Content of chlorophyll b.
The actual value of table 1 jujube tree canopy chlorophyll b adds up (n=50) with detected value
The above, the only present invention preferably detailed description of the invention, protection scope of the present invention is not limited to this, any is familiar with basis Those skilled in the art in the technical scope of present disclosure, the simple change of the technical scheme that can become apparent to Or equivalence replacement each falls within protection scope of the present invention.

Claims (5)

1. the detection method of a jujube tree canopy content of chlorophyll b, it is characterised in that comprise the following steps:
(1) sunny, cloudless or a small amount of cloud layer, the wind-force weather less than 3 grades is selected to gather required mensuration content of chlorophyll b jujube tree hat The hyperspectral information of layer;
(2), after high-spectral data being carried out multiplicative scatter correction, use wavelet de-noising, then be smoothed;
(3) extract the 470 of content of chlorophyll b jujube tree canopy to be determined, 610,645,652,663,985,1160nm wavelength Spectral reflectance values;
(4) reflectance value of each wavelength is substituted into jujube tree canopy content of chlorophyll b detection model:
Y=0.53796-30.89546x1-44.00625x2+506.2439x3-735.3674x4+289.2066x5-4.507388x6+8.1820 25x7
Wherein, y is jujube tree canopy content of chlorophyll b, and unit is mg/g, x1、x2、x3、x4、x5、x6、x7It is respectively Fructus Jujubae Crown canopy 470,610,645,652,663,985, the reflectance value of 1160nm wave band.
The detection method of jujube tree canopy content of chlorophyll b the most according to claim 1, it is characterised in that step (1) Described in the hyperspectral information collection of jujube tree canopy, during collection, probe vertically downward, and must need to be directed at canopy center, probe Away from 1 meter of height of canopy.
The detection method of jujube tree canopy content of chlorophyll b the most according to claim 1, it is characterised in that step (1) Described in the hyperspectral information collection of jujube tree canopy, its acquisition time is Beijing time 11:00-15:00.
The detection method of jujube tree canopy content of chlorophyll b the most according to claim 1, it is characterised in that step (2) Described in smoothing processing method be Savitzky-Golay Smoothing, smooth window is 7.
The detection method of jujube tree canopy content of chlorophyll b the most according to claim 1, it is characterised in that step (4) Described in 470,610,645,652,663,985, the spectral reflectance values of 1160nm wavelength, surveyed by same canopy 10 repetitions reflectance averagely after and value after multiplicative scatter correction, wavelet de-noising and smoothing processing.
CN201610361783.5A 2016-05-23 2016-05-23 Method for detecting content of chlorophyll b in canopy of jujube tree Pending CN105954207A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106248627A (en) * 2016-10-09 2016-12-21 塔里木大学 A kind of detection method of the humus content of the soil
CN106501454A (en) * 2016-10-31 2017-03-15 塔里木大学 A kind of satellite remote-sensing monitoring method of jujube tree canopy content of chlorophyll b
CN107132204A (en) * 2017-06-08 2017-09-05 浙江大学 The measurement apparatus and inversion method of a kind of chlorophyll content in leaf blades
CN107219226A (en) * 2017-07-24 2017-09-29 中国科学院遥感与数字地球研究所 Image collecting device and enhancing vegetation index monitoring system
CN111175783A (en) * 2019-12-31 2020-05-19 塔里木大学 Satellite remote sensing monitoring method for cotton canopy chlorophyll b content

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CN103674838A (en) * 2013-11-12 2014-03-26 浙江大学 Hyperspectral imaging technology-based fish fat content distribution detection method
CN104483274A (en) * 2015-01-17 2015-04-01 华东交通大学 Device and method for detecting leaf nitrogen concentration of field maize plants

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106248627A (en) * 2016-10-09 2016-12-21 塔里木大学 A kind of detection method of the humus content of the soil
CN106501454A (en) * 2016-10-31 2017-03-15 塔里木大学 A kind of satellite remote-sensing monitoring method of jujube tree canopy content of chlorophyll b
CN107132204A (en) * 2017-06-08 2017-09-05 浙江大学 The measurement apparatus and inversion method of a kind of chlorophyll content in leaf blades
CN107219226A (en) * 2017-07-24 2017-09-29 中国科学院遥感与数字地球研究所 Image collecting device and enhancing vegetation index monitoring system
CN111175783A (en) * 2019-12-31 2020-05-19 塔里木大学 Satellite remote sensing monitoring method for cotton canopy chlorophyll b content

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