CN104502285A - Device and method for detecting content of protein in tomato leaves by multi-angle in situ spectrum - Google Patents
Device and method for detecting content of protein in tomato leaves by multi-angle in situ spectrum Download PDFInfo
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- CN104502285A CN104502285A CN201410794855.6A CN201410794855A CN104502285A CN 104502285 A CN104502285 A CN 104502285A CN 201410794855 A CN201410794855 A CN 201410794855A CN 104502285 A CN104502285 A CN 104502285A
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Abstract
The invention relates to the technical field of spectrum detection, and particularly relates to a device and a method for detecting the content of protein in tomato leaves by multi-angle in situ spectrum for tomato planting. The device comprises a spectrum probe and a light source, and further comprises an all-dimensional gyroscope and a light insulating device, wherein the all-dimensional gyroscope consists of an arc-shaped suspension plate, a circular ring track plate, an electric-driven roller and landing legs. The method comprises the following steps: (a) preparing for equipment; (b) correcting a spectrum white board; (c) automatically adjusting and sampling an angle of a leaf spectrum; (d), multi-sampling the leaf spectrum; and (e) calculating a physicochemical value. The device is arranged in a tomato planting field, and can obtain spectrum data of the content of the protein in the alive tomato leaves. The tomato leaves can be measured from multiple angles in an all-dimensional manner; the electric-driven roller automatically rotates, and meanwhile, a camera is matched for performing observation monitoring, so that the detection efficiency is high.
Description
Technical field
The present invention relates to technical field of spectral detection, particularly relate to the spectral device for tomato planting Site Detection tomato leaf protein content.
Background technology
Tamato fruit contains the various nutrient elements such as a large amount of carbohydrates, protein, amino acid, vitamin and inorganic salts.So tomato is welcome by common people in China very much, annual output is very large.In tomato plant strain growth process; the free radical that protein content in blade can produce resisting abiotic stress; for plant provides protection; protein content index in monitoring tomato growth process; analyze the reason causing protein content index to change; for tomato planting decision-making provides foundation, provide scientific basis to the reasonable water and fertilizer management of tomato planting.
Tomato leaf protein content determination still mainly chemical assay measure.Adopt chemical assay time and effort consuming, need more human cost, analytic process has more mechanical manipulation, and is generally not suitable for analyzing great amount of samples; Chemical analysis needs to use chemical reagent, to environment, certainly will take into account the accuracy of determination data, simple operation and environment friendly and pollution-free characteristic, utilize spectroscopy technology substitute conventional chemical analysis method application to a certain extent.
As shown in Figure 1, because tomato leaf growthform is different, in-site measurement requires very high to light source projects angle and spectroscopic probe head incident angle, the method that existing tomato leaf protein spectral detects adopts harvesting young leaves mostly, the fresh-keeping accuracy preventing blade from going bad to affect protein spectral measurement of pack trash ice is high to measurement protein content requirement of real-time.
Summary of the invention
For the problems referred to above, the object of the present invention is to provide and be applicable to Site Detection, measure the tomato leaf of multi-angle In situ spectroscopic detection accurately protein content apparatus and method.
For reaching above-mentioned purpose, the technical solution used in the present invention is: multi-angle In situ spectroscopic detects tomato leaf protein content device, include spectroscopic probe head, light source, its difference is, also include all-directional rotation instrument and every electro-optical device, all-directional rotation instrument is by circular arc suspension plate, ring ring plate, electricity drives roller, supporting leg forms, circular arc suspension plate arc uniform arranges row's mounting hole, light source and spectroscopic probe head select one of them mounting hole to be connected in circular arc suspension plate according to crevice projection angle and incident angle respectively, circular arc suspension plate two head end is equipped with electricity and drives roller, ring ring plate is provided with the groove of opening upwards, electricity drives in roller embedding groove and advances along impression orbit, ring ring plate bottom surface is connected with passive supporting leg, during detection, tomato strain alive is in ring ring plate center, is entered wherein by all-directional rotation instrument every electro-optical device together with tested tomato strain cover alive.
Preferably, describedly include light shade cover, support every electro-optical device, light shade cover is made by every luminescent material, and top has string holes, and cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, and string holes place is provided with sealing-plug.
Preferably, described circular arc suspension plate is also connected with camera.
Preferably, described electricity drives roller and is made up of roller and stepper motor, and stepper motor output shaft is connected with roller wheel shaft.
Preferably, the adjustable bolt regulating Circular Plate terrain clearance is provided with between described supporting leg and Circular Plate.
Preferably, described circular arc suspension plate semicircular in shape, and the scale mark representing suspension plate arc angle is marked with at sidewall.
Preferably, the mounting hole number of described circular arc suspension plate is 18, and adjacent holes angle is 10 °.
Preferably, describedly also include electric block every electro-optical device, light shade cover top is provided with suspension ring, and the suspension hook of electric block coordinates with suspension ring, and electric block is suspended on support.
Preferably, described support is three-legged support.
Multi-angle In situ spectroscopic detects tomato leaf method of protein: comprise the steps:
A () equipment prepares: erection bracket, lifting light shade cover, set up all-directional rotation instrument and level leveling, tomato strain alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively by mounting hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover;
B () spectrum blank is corrected: blank and the concordant placement of tomato strain blade height alive, carries out the rectification of spectrum blank;
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller and starts, circular arc suspension plate is driven to turn over certain angle together with spectroscopic probe head and light source, and coordinate adjustment ring ring plate height, consistent to ensure the light path of check point by camera observation monitoring, incident angle and reflection angle are 90 °; Light source sends to first check point and detects light, and the wavelength utilizing spectroscopic probe head acquisition testing point place is 690 nm, the reflectivity of tomato leaf corresponding to 850 nm, 992 nm, 1300 nm, 1500 nm, 1600 nm, 1984 nm, 2248 nm and 2304 nm;
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other check point;
E () physics and chemistry value calculates: by gained three point reflection rates Input Control Element respectively, control module is according to multiple linear regression equations:
Y=19.852X1+14.478X2+31.258X3-24.334X4+29.374X5-8.698X6+2 5.698X7+31.258X8-62.365X9+5.39, calculate the protein content of tomato leaf, in formula, Y is protein content in tomato leaf, X1 ~ X9 is respectively wavelength 690 nm, 850 nm, 992 nm, 1300 nm, 1500 nm, 1600 nm, 1984 nm, the reflectivity of 2248 nm and the strain blade alive of the tomato corresponding to 2304 nm, three check points obtain three protein content values, Y1, Y2 and Y3, by Y1, Y2 and Y3 summation is made even and is protein content Y in tomato leaf.
Beneficial effect of the present invention has:
(1) all-directional rotation instrument is at tomato planting erected on site, can obtain the spectroscopic data of strain tomato leaf protein alive, avoids because of the in vitro rear inaccurate problem of tomato leaf protein content data.
(2) circular arc suspension plate is adopted to rotate at ring ring plate level, and select different suspension angles, and tomato leaf can be measured in all-dimensional multi-angle ground, can according to the self-sow form of different tomato leaf, change spectrum crevice projection angle and incident angle easily, obtain and preferably take measurement of an angle.
(3) adjustable bolt regulates Circular Plate terrain clearance, can according to different plant growths height, and spectral preservation probe and crop distance relative constancy, to obtain preferably spectroscopic data.
(4) use the light shade cover made every luminescent material by testing tool together with tested crop cover into, stop that external environment is on the impact of spectroscopic data, it is more accurate to measure.
(5) do not lifting by crane in electro-optical device situation, electricity drives roller and automatically drives circular arc suspension plate to rotate at ring ring plate level, camera coordinates observation monitoring consistent to ensure the light path of check point simultaneously, solution drives roller without electricity and camera must repeatedly lift by crane every electro-optical device, the problem that detection efficiency is low.
(6) whole device is simple and practical, with low cost, is easy to promote.
Accompanying drawing explanation
Fig. 1 is tomato leaf schematic diagram;
Fig. 2 is all-directional rotation instrument structural representation in the embodiment of the present invention;
Fig. 3 is every electro-optical device structural representation in the embodiment of the present invention;
Fig. 4 implements in the embodiment of the present invention to measure tomato leaf protein content result;
In figure, mark indicates: 1-camera, 2-circular arc suspension plate, 3-spectroscopic probe head, 4-groove, 5-ring ring plate, 6-electricity drives roller, 7-adjustable bolt, 8-supporting leg, 9-light source, 10-support, 11-string holes, 12-light shade cover, 13-electric block, 14-light source connecting seat, 15-camera Connection Block, 16-spectroscopic probe head Connection Block, the strain alive of 17-tomato.
Embodiment
In order to understand the present invention better, below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described further, see Fig. 2, Fig. 3 and Fig. 4:
Tomato leaf protein content device is detected by multi-angle In situ spectroscopic of the invention process, Main Function facilitates erected on site, and different self-sow form tomato leaf can be suitable for, so adopt all-directional rotation instrument to hang spectroscopic probe head 3 and light source 9, to change spectrum crevice projection angle and incident angle rapidly, obtain and preferably take measurement of an angle.Stop that external environment is on the impact of spectroscopic data every electro-optical device, it is more accurate to measure.
The structure that all-directional rotation instrument is concrete: comprise circular arc suspension plate 2, ring ring plate 5, electricity drive roller 6, supporting leg 8, circular arc suspension plate 2 arc uniform arranges row's mounting hole, spectroscopic probe head 3 and light source 9 select one of them mounting hole respectively according to crevice projection angle and incident angle, are connected in circular arc suspension plate 2 respectively by light source connecting seat 14 and spectroscopic probe head Connection Block 16.Take measurement of an angle preferably to find rapidly, in the present embodiment, mounting hole adds up to 18, and every adjacent holes angle is 10 °.In order to record matching measurement result, be marked with the scale mark representing suspension plate arc angle at circular arc suspension plate 2 sidewall of semicircular in shape.Circular arc suspension plate 2 liang of head ends are equipped with electricity and drive roller 6, ring ring plate 5 is hollow annular structure, which is provided with the groove 4 of opening upwards, electricity drives roller 6 and embeds in groove 4 and advance along its track, promote circular arc suspension plate 2 to coexist ring ring plate 5 Plane Rotation together with spectroscopic probe head 3 one, pass through the mounting hole adjustment light source 9 of circular arc suspension plate 2 and the incident angle of spectroscopic probe head 3 and reflection angle is 90 ° simultaneously, reaches the object that all-dimensional multi-angle is measured.
Ring ring plate 5 bottom surface is connected with passive supporting leg 8, in order to according to different plant growths height, spectral preservation probe 3 with crop distance relative constancy, to obtain preferably spectroscopic data.Between supporting leg 8 and ring ring plate 5, the adjustable bolt 7 regulating ring ring plate 5 terrain clearance is provided with in the present embodiment.
Stop external environmental light every electro-optical device, during detection, tomato strain 17 of living is in ring ring plate 5 center, and all-directional rotation instrument covers into wherein together with the strain 17 of living of tested tomato by light shade cover 12.Describedly be made up of light shade cover 12, support 10 every electro-optical device, light shade cover 12 is made by every luminescent material, and top has string holes 11, and the cable of spectroscopic probe head 3 and light source 9 and data line pass from string holes 11, and string holes 11 place is provided with sealing-plug.Also include electric block 13 every electro-optical device, light shade cover 12 top is provided with suspension ring, and the suspension hook of electric block 13 coordinates with suspension ring, and electric block 13 hangs over the mount 10.Described support 10 is three-legged support 10.Light shade cover 12 covers above, in order to understand inner case in real time better, is provided with camera 1 in the present embodiment in circular arc suspension plate 2 by camera Connection Block 15.
During embodiment of the present invention work, detect the method for tomato leaf protein content, comprise the steps:
A () equipment prepares: erection bracket 10, lifting light shade cover 12, set up all-directional rotation instrument and level leveling, tomato strain 17 alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively by mounting hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover;
B () spectrum blank is corrected: blank and the concordant placement of tomato strain 17 blade height alive, carries out the rectification of spectrum blank;
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller 6 and starts, circular arc suspension plate 2 is driven to turn over certain angle together with spectroscopic probe head 3 and light source 9, and coordinate adjustment ring ring plate 5 height, observe monitoring consistent to ensure the light path of check point by camera 1, incident angle and reflection angle are 90 °; Light source 9 sends to first check point and detects light, the reflectivity of the wavelength utilizing spectroscopic probe head 3 acquisition testing point place is 690 nm, tomato corresponding to 850 nm, 992 nm, 1300 nm, 1500 nm, 1600 nm, 1984 nm, 2248 nm and 2304 nm lives strain 17 blade;
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other check point;
E () physics and chemistry value calculates: by gained three point reflection rates Input Control Element respectively, control module is according to multiple linear regression equations:
Y=19.852X1+14.478X2+31.258X3-24.334X4+29.374X5-48.698X6+25.698
X7+31.258X8-62.365X9+5.39, calculate the protein content of tomato strain 17 blade alive, in formula, Y is protein content in tomato leaf, X1 ~ X9 is respectively the reflectivity of wavelength 690 nm, 850 nm, 992 nm, 1300 nm, 1500 nm, 1600 nm, 1984 nm, 2248 nm and the tomato corresponding to 2304 nm strain 17 blade alive, three check points obtain three protein content values, Y1, Y2 and Y3, Y1, Y2 and Y3 summation is made even and is protein content Y in tomato leaf.
Say from microcosmic, the active state of molecule of composition tomato leaf is subject to the energy excitation detecting light, produces different energy level transitions, absorbs and release energy, and forms corresponding with molecule reflectance spectrum and absorption spectrum.Different chemical bonds, as O-H, N-H, C-H etc., the rotation of chemical bond and stretching vibration, can absorb the light of specific wavelength in transition between energy level, and excite generation characteristic peak, and the intensity of foundation characteristic peak and wave number can judge the composition of material.
The vibration information of the chemical bonds such as N-H, C-N, N-H in the protein of wavelength used and tomato leaf and related chemical constituents is relevant, and these information can reflect the power of protein content in pears tomato leaf.Such as 690 nm and 850 nm reflect aromatic hydrocarbons and the methyl information of tomato leaf protein, and 992 nm reflect the amido information of tomato leaf protein, and 1300 nm reflect the group CH of tomato leaf protein
3information, 1500 nm reflect the group NH of tomato leaf protein
2information, 1600 nm reflect the group ArCH information of tomato leaf protein, and 1984 nm reflect tomato leaf protein group CONH
2information and primary amide information, 2248 nm and 2304 nm reflect tomato leaf protein group CH
2information.Application arithmetic of linearity regression carries out modeling analysis to above-mentioned characteristic wavelength, sets up the protein content of tomato leaf and the linear equation of characteristic wavelength.
The present invention the characteristic wavelength selected only for the quick detection of the protein content of tomato leaf, if need to measure other indexs, need again to select specific wavelength, by multiple linear regression, Modling model, calculates corresponding desired value.
Utilize the index of correlation of method establishment model provided by the invention in table 1, wherein, during modeling set representations Modling model, utilize the reflectivity of tomato leaf and existing method to test the tomato leaf protein content matching obtained and obtain model equation, forecast set represents, during prediction, after namely having set up model, according to model equation, the characteristic wavelength reflectivity of tomato leaf spectrum is substituted into the tomato leaf protein content that model equation calculates.
Table 1
| Data set | Number of samples | The coefficient of determination | Root-mean-square error (mg/g) |
| Modeling collection | 100 | 0.8502 | 3.158 |
| Forecast set | 40 | 0.8412 | 3.214 |
Embodiment 1 ~ 10
Utilize apparatus and method provided by the invention to test tomato leaf protein content, test result is in table 2.
Table 2
By table 2 data, can find out, it is close that the protein content of the tomato leaf utilizing the inventive method to record and existing method test the data obtained, and utilizes the present invention can the protein content of Fast Measurement tomato leaf, reliable results.
Claims (10)
1. multi-angle In situ spectroscopic detects tomato leaf protein content device, include spectroscopic probe head, light source, it is characterized in that, also include all-directional rotation instrument and every electro-optical device, all-directional rotation instrument is by circular arc suspension plate, ring ring plate, electricity drives roller, supporting leg forms, circular arc suspension plate arc uniform arranges row's mounting hole, light source and spectroscopic probe head select one of them mounting hole to be connected in circular arc suspension plate according to crevice projection angle and incident angle respectively, circular arc suspension plate two head end is equipped with electricity and drives roller, ring ring plate is provided with the groove of opening upwards, electricity drives in roller embedding groove and advances along impression orbit, ring ring plate bottom surface is connected with passive supporting leg, during detection, tomato strain alive is in ring ring plate center, is entered wherein by all-directional rotation instrument every electro-optical device together with tested tomato strain cover alive.
2. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf protein content device, it is characterized in that: describedly include light shade cover, support every electro-optical device, light shade cover is made by every luminescent material, top has string holes, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, and string holes place is provided with sealing-plug.
3. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf protein content device, it is characterized in that: described circular arc suspension plate is also connected with camera.
4. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf protein content device, it is characterized in that: described electricity drives roller and is made up of roller and stepper motor, and stepper motor output shaft is connected with roller wheel shaft.
5. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf protein content device, it is characterized in that: be provided with the adjustable bolt regulating Circular Plate terrain clearance between described supporting leg and Circular Plate.
6. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf protein content device, it is characterized in that: described circular arc suspension plate semicircular in shape, and is marked with the scale mark representing suspension plate arc angle at sidewall.
7. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf protein content device, it is characterized in that: the mounting hole number of described circular arc suspension plate is 18, and adjacent holes angle is 10 °.
8. multi-angle In situ spectroscopic according to claim 2 detects tomato leaf protein content device, it is characterized in that: describedly also include electric block every electro-optical device, light shade cover top is provided with suspension ring, and the suspension hook of electric block coordinates with suspension ring, and electric block is suspended on support.
9. multi-angle In situ spectroscopic according to claim 2 detects tomato leaf protein content device, it is characterized in that: described support is three-legged support.
10. multi-angle In situ spectroscopic detects the method for tomato leaf protein content, it is characterized in that: comprise the steps:
A () equipment prepares: erection bracket, lifting light shade cover, set up all-directional rotation instrument and level leveling, tomato strain alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively by mounting hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover;
B () spectrum blank is corrected: blank and the concordant placement of tomato strain blade height alive, carries out the rectification of spectrum blank;
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller and starts, circular arc suspension plate is driven to turn over certain angle together with spectroscopic probe head and light source, and coordinate adjustment ring ring plate height, consistent to ensure the light path of check point by camera observation monitoring, incident angle and reflection angle are 90 °; Light source sends to first check point and detects light, and the wavelength utilizing spectroscopic probe head acquisition testing point place is 690 nm, the reflectivity of tomato leaf corresponding to 850 nm, 992 nm, 1300 nm, 1500 nm, 1600 nm, 1984 nm, 2248 nm and 2304 nm;
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other check point;
E () physics and chemistry value calculates: by gained three point reflection rates Input Control Element respectively, control module is according to multiple linear regression equations:
Y=19.852X1+14.478X2+31.258X3-24.334X4+29.374X5-8.698X6+2 5.698X7+31.258X8-62.365X9+5.39, calculate the protein content of tomato leaf, in formula, Y is protein content in tomato leaf, X1 ~ X9 is respectively wavelength 690 nm, 850 nm, 992 nm, 1300 nm, 1500 nm, 1600 nm, 1984 nm, the reflectivity of 2248 nm and the strain blade alive of the tomato corresponding to 2304 nm, three check points obtain three protein content values, Y1, Y2 and Y3, by Y1, Y2 and Y3 summation is made even and is protein content Y in tomato leaf.
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| CN104568847A (en) * | 2014-12-21 | 2015-04-29 | 华东交通大学 | Device and method for detecting activity of catalase of tomato leaves by multi-angle in-situ spectrum |
| CN108562687A (en) * | 2018-03-30 | 2018-09-21 | 山西省农业科学院农作物品种资源研究所 | A kind of wheat Siargos genes albumen comparison device |
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| CN108562687B (en) * | 2018-03-30 | 2021-05-04 | 山西省农业科学院农作物品种资源研究所 | Wheat Siargos is protein contrast device for gene |
| CN112720717A (en) * | 2018-10-24 | 2021-04-30 | 姚育晶 | Wood clamping system and method |
| CN112720717B (en) * | 2018-10-24 | 2022-04-01 | 淮北特致信息科技有限公司 | Wood clamping system and method |
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Effective date of registration: 20160906 Address after: 330013 Jiangxi city of Nanchang Province Economic and Technological Development Zone East Shuanggang Street No. 808 Applicant after: East-China Communication Univ Applicant after: Jiangxi Huada Engineering Quality Inspection Co., Ltd. Applicant after: Liu Xuemei Address before: 330013 Jiangxi city of Nanchang Province Economic and Technological Development Zone East Shuanggang Street No. 808 Applicant before: East-China Communication Univ Applicant before: Zhang Hailiang Applicant before: Liu Xuemei |
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