CN108562687A - A kind of wheat Siargos genes albumen comparison device - Google Patents
A kind of wheat Siargos genes albumen comparison device Download PDFInfo
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- CN108562687A CN108562687A CN201810294008.1A CN201810294008A CN108562687A CN 108562687 A CN108562687 A CN 108562687A CN 201810294008 A CN201810294008 A CN 201810294008A CN 108562687 A CN108562687 A CN 108562687A
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- wheat
- albumen
- bar
- siargos
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Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 39
- 241000209140 Triticum Species 0.000 title claims abstract description 32
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 31
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 50
- 230000007246 mechanism Effects 0.000 claims abstract description 37
- 238000012360 testing method Methods 0.000 claims abstract description 29
- 238000010438 heat treatment Methods 0.000 claims abstract description 28
- 238000001816 cooling Methods 0.000 claims abstract description 15
- 238000009423 ventilation Methods 0.000 claims abstract description 12
- 238000002474 experimental method Methods 0.000 claims abstract description 9
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 7
- 238000006073 displacement reaction Methods 0.000 claims description 15
- 230000001483 mobilizing effect Effects 0.000 claims description 15
- 239000010425 asbestos Substances 0.000 claims description 6
- 230000008878 coupling Effects 0.000 claims description 6
- 238000010168 coupling process Methods 0.000 claims description 6
- 238000005859 coupling reaction Methods 0.000 claims description 6
- 229910052895 riebeckite Inorganic materials 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000017105 transposition Effects 0.000 claims 1
- 238000003805 vibration mixing Methods 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 23
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 102000004169 proteins and genes Human genes 0.000 description 11
- 238000010586 diagram Methods 0.000 description 8
- 244000062793 Sorghum vulgare Species 0.000 description 7
- 230000014509 gene expression Effects 0.000 description 7
- 235000019713 millet Nutrition 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 210000000056 organ Anatomy 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 239000004327 boric acid Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000003375 plant hormone Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000002331 protein detection Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- 108700002572 Arabidopsis ARGOS Proteins 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 108091062157 Cis-regulatory element Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003766 bioinformatics method Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000009841 combustion method Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000030214 innervation Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical class [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000001963 scanning near-field photolithography Methods 0.000 description 1
- 238000012882 sequential analysis Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/002—Determining nitrogen by transformation into ammonia, e.g. KJELDAHL method
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of wheat Siargos genes albumen to compare device, including testing pedestal, the upper end of the experiment pedestal is equipped with test panel, the upper surface of the test panel is equipped with disintegrating mechanism and comparison mechanism body, the front side of the comparison mechanism body, which is equipped with, tilts locking frame, and described tilt is additionally provided with triangle placement plate on front side of locking frame, the heating mechanism that the comparison mechanism body includes the locking plate of reagent bottle and is arranged below the locking plate of reagent bottle, the lower surface of the test panel is equipped with cooling fan, the test panel is equipped with ventilation hollow slots on the position of cooling fan；This programme is multiple functional, collection crushes wheat and albumen powder, solution vibration mixing and reagent bottle is taken to be supported in one, stable connection between compact-sized and structure, albumen contrast operation is conveniently completed, it is highly practical, using multi-angle rotary mechanism, it is used suitable for different operation step, and structure member is at low cost, using simple, facilitates arrangement.
Description
Technical field
The present invention relates to Siargos gene applied technical fields, specially a kind of wheat Siargos genes are compared with albumen
Device.
Background technology
Crop yield character and plant organ's size there are substantial connection, plant organ size except be illuminated by the light, temperature, nutrition
It influences outside, mainly to be regulated and controled by genes such as gene expression and signal transductions with external environmental conditions such as hormones.Wheat has resistance to
Non-irrigated barren tolerance is strong, base group is small, diploid, short breeding time the features such as, be that mode crop is carried out functional gene and studied using wheat, can be
It solves the drought resisting for being difficult to parse for many years and C4 photosynthesis genetic molecule mechanism provides brand-new opportunity.Therefore, control is excavated
The critical function gene of wheat organ size is simultaneously used for genetic improvement, for accelerating wheat breeding process and improving millet per unit area yield tool
It plays an important role.
Current existing scientific achievement is shown, is detached from wheat by hormone induced expression, is participated in the control of organ size
The homologous gene of arabidopsis ARGOS (Auxin-regulatedgeneinvolvedinorgansize) gene family, is given birth to
Object bioinformatics analysis specifies it in different tissues organ and its expression pattern induced by plant hormone, analyzes gene coding region
And its promoter sequence difference, development function label, the improvement for millet yield traits related gene provide foundation.By to
There are ARGOS albumen conserved domains to carry out BLAST, specifies millet ARGOS family members number and carry out sequential analysis of protein, adopt
One of millet ARGOS family members are obtained with homologous clone method --- the code areas SiARGOS1 and its promoter sequence, with life
Object Informatics Method analyzes the cis-acting elements of SiARGOS1 promoters, analyzes the gene by real-time fluorescence quantitative PCR and exists
Induced expression pattern in each organ of millet and under the conditions of different plant hormone utilizes gene coding region and promoter sequence
SNP and insertion and deletion sequence develop molecular labeling.
The code areas SiARGOS1 and its promoter sequence, the gene are located on the 8th chromosome of millet, and open reading frame is
342bp, intronless, encode 113 amino acid, promoter region 2109bp, containing with auxin, ethylene, jasmonic and
The related element of the various plants hormone regulating and controlling such as gibberellin.Expression analysis finds that SiARGOS1 is in millet root, stem, leaf and fringe etc.
There is expression in organ, secondly the expression quantity highest in root is stem and leaf, expression quantity is minimum in fringe.
It influences, is needed to difference in order to study protein content of the code areas SiARGOS1 in the wheat wheat head of different cultivars
The wheat of kind carries out albumen and compares device, but the albumen comparison apparatus structure being widely used at present fix it is unadjustable,
Practicability is low, is not easy to use under the conditions of a variety of comparisons, and in addition only there is general albumen comparison device reagent fusion to occur
The function of reaction, and there is no that tool is available for sampling and reagent fusion steps, it needs in addition to add equipment, seriously affects reality
Test comparison working efficiency.
Invention content
In order to overcome the shortcomings of that prior art, the present invention provide a kind of wheat Siargos genes albumen comparison dress
It sets, multiple functional, collection crushes wheat and albumen powder, solution vibration mixing and reagent bottle is taken to be supported in one, compact-sized and tie
Stable connection between structure conveniently completes albumen contrast operation, highly practical, using multi-angle rotary mechanism, is suitable for
Different operation step uses, and structure member is at low cost, using simple, facilitate arrangement, can effectively solve the problem that background technology carries
The problem of going out.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of wheat Siargos genes albumen comparison device, including experiment pedestal, the upper end of the experiment pedestal are equipped with
The upper surface of test panel, the test panel is equipped with disintegrating mechanism and compares mechanism body, before the comparison mechanism body
Side, which is equipped with, tilts locking frame, and described tilt is additionally provided with triangle placement plate, the comparison mechanism body packet on front side of locking frame
The lower surface of the heating mechanism for including the locking plate of reagent bottle and being arranged below the locking plate of reagent bottle, the test panel is equipped with cooling
Fan, the test panel are equipped with ventilation hollow slots on the position of cooling fan；
Four angles of the locking plate of reagent bottle are equipped with elastic rod, and the lower end of the elastic rod is equipped with suit cavity cylinder,
The upper end of the suit cavity cylinder is equipped with locking cap eaves board, is fixed with and the matched support of elastic rod in the test panel
The upper surface of bar, the supporting rod is equipped with embedded fixing groove, and the suit cavity cylinder is fixedly mounted in embedded fixing groove, described
The outside of elastic rod is equipped with torsionspring, and the lower end of the torsionspring is fixedly welded on supporting rod, and the test panel exists
The outer fix of supporting rod is additionally provided with recessed mounting groove, is equipped with vibrating motor in the recessed mounting groove, the vibrating motor is set
There is the vibration rocking bar being connect with supporting rod.
As a kind of preferred technical solution of the present invention, the heating mechanism include heating web plate, displacement mobilizing board and
Hot plate between heating web plate and displacement mobilizing board, four Angle Positions of the heating web plate and displacement mobilizing board are set
It is equipped with screw thread clip, and the heating web plate is equipped with soaking asbestos gauge, the displacement mobilizing board is equipped between several wait
Non-slip mat away from distribution, the hot plate are placed in non-slip mat.
As a kind of preferred technical solution of the present invention, the disintegrating mechanism includes dnockout cylinder and crushes three leaf knives, described
The bottom of dnockout cylinder is equipped with drive motor, and three leaf knives of the crushing are fixedly mounted on the swingle of drive motor, the dnockout
The lower end of cylinder is equipped with discharge port, and the outside of the discharge port is equipped with striker plate, and the both sides of the discharge port are equipped with limit cavity
Plate.
As a kind of preferred technical solution of the present invention, it is equipped in the test panel for tilting locking frame position
Sliding rail, described to tilt locking frame include vertical upright bar and rotation snap-gauge, it is hinged on the rotation snap-gauge there are two semidefinite arc
The inside of shape plate, the semidefinite arc panel is equipped with anti-skid rubber cushion, and the end of the semidefinite arc panel is solid equipped with screw thread
Fixed board is equipped with internal thread hole on the plate that is screwed.
As a kind of preferred technical solution of the present invention, the upper end of the vertical upright bar is equipped with arch sheet, the revotating card
The lower surface of plate upper end, which is fixedly welded with, tightens bolt, the outside of the arch sheet is equipped with matching nut, the bolt of tightening
End, which is fixedly connected with to hold, turns disk.
As a kind of preferred technical solution of the present invention, the lower end of the vertical upright bar is also hinged with check lever, described to stop
The end for returning bar is connected with outer Coupling, also fixed on the arch sheet of the vertical upright bar to paste angled marking plate.
As a kind of preferred technical solution of the present invention, the locking plate of reagent bottle is equipped with the length of several equidistantly distributeds
Shape walks slot, and the side of the elongated wears groove is fixedly welded with undertaking column support, and the undertaking column support is equipped with spiraled cavity
Plate, the spiraled cavity plate is interior to be equipped with rotating clamp, is equipped between the rotating clamp and spiraled cavity plate and tightens bolt, and is described
Rotating clamp is connected with reagent bottle clamping piece.
As a kind of preferred technical solution of the present invention, the triangle placement plate includes two pieces of anti-off boards being hinged,
The other end of the anti-off board also is provided with Bolt plate, and the bottom of the anti-off board is fixed with straight trip connecting rod, the straight trip connecting rod
Upper hinged there are three the spike bar of equidistantly distributed, the upper end of the spike bar is equipped with rotary cylinder.
As a kind of preferred technical solution of the present invention, firm bar is equipped between three spike bars, it is described firm
The centre of bar is equipped with hinge bar, and the end of the firm bar is equipped with the cavity fixed cylinder being fixed on spike bar.
As a kind of preferred technical solution of the present invention, keeping out the wind equipped with several equidistantly distributeds in the ventilation hollow slots
Two opposite flanks of plate, the ventilation hollow slots are equipped with mounting hole, and the both ends of the wind shield, which are equipped with, assigns bar.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) present invention is multiple functional, and collection crushes wheat and albumen powder, solution vibration mixing and reagent bottle is taken to be supported in one,
Stable connection between compact-sized and structure conveniently completes albumen contrast operation, highly practical.
(2) present invention uses multi-angle rotary mechanism, is suitable for different operation step and uses, and structure member cost
It is low, using simple, facilitate arrangement.
Description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the locking board mounting structure schematic diagram of reagent bottle of the present invention；
Fig. 3 is the ventilation hollow slots overlooking structure diagram of the present invention；
Fig. 4 is the disintegrating mechanism structural schematic diagram of the present invention；
Fig. 5 is the heating mechanism structural schematic diagram of the present invention；
Fig. 6 is the locking frame structural schematic diagram of inclination of the present invention；
Fig. 7 tightens bolt fastening structure schematic diagram for the present invention's；
Fig. 8 is that the triangle of the present invention places plate structure schematic diagram；
Figure label：
1- tests pedestal；2- test panels；3- disintegrating mechanisms；4- compares mechanism body；5- tilts locking frame；6- triangles are put
Set plate；7- cooling fans；8- ventilation hollow slots；9- elastic rods；10- is set with cavity cylinder；11- locking cap eaves boards；12- supporting rods；
13- embeds fixing groove；14- torsionsprings；The recessed mounting grooves of 15-；16- vibrating motors；17- vibrates rocking bar；18- sliding rails；
19- is elongated to walk slot；20- accepts column support；21- spiraled cavity plates；22- rotating clamps；23- reagent bottle clamping pieces；24- keeps out the wind
Plate；25- mounting holes；26- assigns bar；
301- dnockout cylinders；302- crushes three leaf knives；303- drive motors；304- discharge ports；305- striker plates；306- is limited
Cavity plate；
The locking plate of 401- reagent bottles；402- heating mechanisms；4021- heats web plate；4022- displacement mobilizing boards；4023- is heated
Electric furnace；4024- screw thread clips；4025- soaking asbestos gauges；4026- non-slip mats；
The vertical upright bars of 501-；502- rotates snap-gauge；503- semidefinite arc panels；504- anti-skid rubber cushions；505- is screwed
Plate；506- internal thread holes；507- arch sheets；508- tightens bolt；509- matches nut；510- is hand-held to turn disk；511- non-returns
Bar；The outer Couplings of 512-；513- angle marking plates；
601- anti-off boards；602- Bolt plates；603- straight trip connecting rods；604- spike bars；605- rotary cylinders；606- consolidates bar；
607- hinge bars；608- cavity fixed cylinders.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1, the present invention provides a kind of wheat Siargos genes albumen to compare device, including experiment pedestal
1, the upper end of the experiment pedestal 1 is equipped with test panel 2, and the upper surface of the test panel 2 is equipped with disintegrating mechanism 3 and comparison machine
The wheat grain of the different cultivars of Siargos gene clonings can be carried out being polished into powder, is convenient for by structure ontology 4, the disintegrating mechanism 3
It is reacted with other reagents, so as to quickly measure the protein content of different wheats in comparison mechanism body 4.
It should be added that the assay method of protein have spectrophotometry, Kjeldahl's method and combustion method etc. its
His mode, mainly uses in the present invention Kjeldahl's method, principle to be：Protein in food is divided in the case where being catalyzed heating condition
Solution, the ammonia of generation is combined with sulfuric acid generates ammonium sulfate, and alkalization distillation keeps ammonia free, with sulfuric acid or normal hydrochloric acid after being absorbed with boric acid
Volumetric soiutions titrate, and conversion coefficient, the as content of protein are multiplied by according to the consumption of acid.
It is as follows to measure the specific operating procedure of protein content：
1. sample pretreating：(1) the solid sample 0.2g~2g mixed well is weighed, (being approximately equivalent to 30mg~40mg nitrogen),
And in addition addition 0.0g does blank assay, is accurate to 0.001g, moves into dry 100mL nitrogen fixing bottles, and 0.2g sulfuric acid is added
Copper, 6g potassium sulfates and 20mL sulfuric acid mixed solutions put a small funnel in bottleneck after jog, bottle are had aperture with the oblique Zhi Yu of 45° angle
Asbestos gauge on, it is careful to heat；
(2) it waits for that content all carbonizes, after foam stops completely, reinforces firepower, and keep liquid slightly boiling in bottle, until liquid
After blue-green and clear, it is further continued for heating 0.5h~1h；
(3) it removes and lets cool, 20mL water is added, after letting cool, move into 100mL volumetric flasks, a small amount of washing nitrogen fixing bottle is used in combination, washes
Liquid is incorporated in volumetric flask, is added water to scale, mixing is spare.
2. measuring：(1) install and determine nitrogen distilling apparatus, to steam evaporator it is built-in 1. in product 10ml-15ml, separately add
Add 0ml to do blank assay, is added several beades, few drops of the red ethanol solution of methylate and several milliliters of sulfuric acid, to keep water in acid
Property, heating boils the water in steam evaporator and keeps boiling；
(2)~2 drop mixing indicator solution of 10.0mL boric acid solutions and 1 drop is added into receiving bottle, and makes the lower end of condenser pipe
It is inserted under liquid level, according to nitrogen content in sample, accurately draws 2.0mL~10.0mL sample treatment solutions and injected by small glass cup and reacted
Room with the small glass cup of 10mL water washings and is allowed to flow into reative cell, then stoppers rodlike glass plug, wherein mixing indicator solution is 2 parts
Methyl red ethanol solution and 1 part of methylene blue ethanol solution.
(3) 10.0mL sodium hydroxide solutions are poured into small glass cup, lifting Bo Sai makes it slowly flow into reative cell, immediately by glass
Corking is tight, and adds water in small glass cup with anti-gas-leak.
(4) mobile distillate receiving bottle after distillation 10min, liquid level leave condenser pipe lower end, redistillation 1min.Then with few
It measures water to rinse outside condenser pipe lower end, removes distillate receiving bottle, titrated to terminal with sulfuric acid or Hydrochloric Standard Titration, face
Color becomes grey by aubergine.
The content calculation formula of protein is in sample：X=[(V-V0)*c*0.0140]/m*F*100。
X：The content (percentage) of protein in sample；
m：Sampling amount；
c：The concentration mol/L of sulfuric acid standard solution；
F：Nitrogen is scaled the coefficient of protein；
V：The volume ml of sample consumption sulfuric acid standard solution；
V0：Reagent blank consumes the volume ml of sulfuric acid standard solution.
As shown in Figures 1 to 4, four angles of the locking plate of the reagent bottle 401 are equipped with elastic rod 9, the elastic rod 9
Lower end is equipped with suit cavity cylinder 10, and the upper end of the suit cavity cylinder 10 is equipped with locking cap eaves board 11, solid in the test panel 2
Fixed be equipped with is equipped with embedded fixing groove 13 with 9 matched supporting rod 12 of elastic rod, the upper surface of the supporting rod 12, and the suit is empty
Chamber cylinder 10 is fixedly mounted in embedded fixing groove 13, and elastic rod 9 has elasticity, can constantly shake under external force, to add
Solvent in fast reagent bottle merges rate, to accelerate the working efficiency of Protein Detection.
The outside of the elastic rod 9 is equipped with torsionspring 14, and the lower both ends of the torsionspring 14 are fixedly welded on support
On bar 12, torsionspring 14 plays the role of support to elastic rod 9, the understrressing that can prevent elastic rod 9 from occurring, when shaking
It tumbles, influences experiment safety, in addition torsionspring 14 also has elasticity, does not influence the shaking effect of elastic rod 9.
The test panel 2 is additionally provided with recessed mounting groove 15, the recessed mounting groove 15 in the outer fix of supporting rod 12
Interior to be equipped with vibrating motor 16, the vibrating motor 16 is equipped with the vibration rocking bar 17 being connect with supporting rod 12, and vibrating motor 16 passes through
Rocking bar 17 is vibrated so that supporting rod 12 generates vibration sense, and vibration sense can make 9 rotational wobble of elastic rod, to accelerate in reagent bottle
Mixed solvent merges rate.
It should be added that in the continuous mode of protein, need to mix different reagents, 16 He of vibrating motor
Elastic rod 9 can rock the locking plate of reagent bottle 401, to by the quick and complete mixing of mix reagent, improve the accuracy of experiment.
It further illustrates, the lower surface of the recessed mounting groove 15 is equipped with several cooling fins, can be risen to vibrating motor
To the effect of heat radiation protection, and it is common straight-bar to vibrate rocking bar 17, and main function is to transmit shaking for the vibration generation of rocking bar 17
The lower end of innervation, supporting rod 12 is fixedly mounted in test panel 2.
The disintegrating mechanism 3 includes dnockout cylinder 301 and crushes three leaf knives 302, and the bottom of the dnockout cylinder 301 is equipped with driving
Motor 303, three leaf knives 302 of the crushing are fixedly mounted on the swingle of drive motor 303, the lower end of the dnockout cylinder 301
Equipped with discharge port 304, the outside of the discharge port 304 is equipped with striker plate 305, and the both sides of the discharge port 304 are equipped with limit
Cavity plate 306, crush three leaf knives 302 quickly rotated under the action of drive motor 303, wheat can be polished into powder, convenient for
It is mixed between reagent, to reduce the protein determination time, striker plate 305 plays the role of sealing dnockout cylinder 301, prevents from polishing
During, wheat flour is unrestrained.
It should be added that in the dnockout cylinder 301 also can adding liquid, be polished into flour paste, and it is described go out
Discharge nozzle can be added in material mouth 304 by threaded engagement, and discharge nozzle is equipped with butterfly valve, controls flow.
The lower surface of the test panel 2 is equipped with cooling fan 7, and the test panel 2 is set on the position of cooling fan 7
There are ventilation hollow slots 8, cooling fan 7 mainly to be cooled down to nitrogen fixing bottle, by adding cross-ventilation, increases cooling efficiency, lead to
Wind hollow slots 8 transmit the air cooling effect of cooling fan 7.
The wind shield 24 of several equidistantly distributeds, two phases of the ventilation hollow slots 8 are equipped in the ventilation hollow slots 8
Mounting hole 25 is equipped with to side, the both ends of the wind shield 24, which are equipped with, assigns bar 26, wind shield 24 can be by assigning bar 26 pacifying
Rotation in hole 25 is filled, the wind direction that wind shield 24 can generate cooling fan 7 is adjusted, can be according to the riding position of reagent bottle
It is specific to adjust wind direction, increase practicability.
As shown in Figure 1, the comparison mechanism body 4 includes the locking plate of reagent bottle 401 and setting in the locking plate of reagent bottle 401
The heating mechanism 402 of lower section, the locking plate of reagent bottle 401 are mainly fixed the steam evaporator during protein measurement,
Heating mechanism 402 can heat steam evaporator, improve temperature, increase chemical reaction efficiency.
The locking plate 401 of reagent bottle is equipped with the elongated of several equidistantly distributeds and walks slot 19, described elongated to walk slot
19 side, which is fixedly welded with, accepts column support 20, and the undertaking column support 20 is equipped with spiraled cavity plate 21, and the spiral is empty
It is equipped with rotating clamp 22 in cavity plate 21, also is provided with tightening bolt 508 between the rotating clamp 22 and spiraled cavity plate 21, and described
Rotating clamp 22 is connected with reagent bottle clamping piece 23, the elongated inclined spaces walked slot 19 and provide steam evaporator, while elongated
Walk slot 19 also can play the role of clamping to steam evaporator.
The side of spiraled cavity plate 21 is openning shape, and rotating clamp 22 can be rotated in spiraled cavity plate 21, to drive reagent
Bottle clip gripping member 23 rotates, and realizes and tilts clamping effect, and tightening bolt 508 can be by the rotating clamp 22 and spiraled cavity plate 21 after rotation
It is fixedly installed togather, to play the role of fixating reagent bottle clip gripping member 23.
It should be added that the reagent bottle clamping piece 23 is common clip, and in reagent bottle clamping piece 23
Inner surface be equipped with non-slip mat, prevent reagent bottle from falling off, increase structural stability and safety in utilization.
Adding as shown in figure 5, the heating mechanism 402 includes heating web plate 4021, displacement mobilizing board 4022 and setting
Hot plate 4023 between heat supply network plate 4021 and displacement mobilizing board 4022, the heating web plate 4021 and displacement mobilizing board 4022
Four Angle Positions be equipped with screw thread clip 4024, and the heating web plate 4021 is equipped with soaking asbestos gauge 4025, described
Displacement mobilizing board 4022 is equipped with the non-slip mat 4026 of several equidistantly distributeds, and the hot plate 4023 is placed on non-slip mat
On 4026, hot plate 4023 is placed on the non-slip mat 4026 on displacement mobilizing board 4022, and non-slip mat 4026 can prevent electric heating electric
Stove 4024 shakes, and increases stability when using, and heating web plate 4021 can play a supporting role to reagent bottle, in addition heat
Soaking asbestos gauge 4025 on web plate 4021, it is ensured that reagent bottle is heated evenly, and is prevented reagent bottle uneven heating from exploding, is added
The screw thread clip 4024 of four Angle Positions of heat supply network plate 4021 and displacement mobilizing board 4022 can be convenient for heating web plate 4021 and displacement
The shift position on supporting rod 12 of mobilizing board 4022 is suitable for various sizes of examination so as to adjust the position of heating web plate 4021
The case where agent bottle also can adjust the position of hot plate 4023, be suitable for different heating degree.
Screw thread clip 4024 is common fixed clamping piece, and the arc semi-circular plate being hinged for two is constituted, arc half
Sealing ring is also added in the inside of plectane, can increase frictional force, while playing the role of anti-scratch, two arcs half to supporting rod 12
The end of plectane is equipped with Bolt plate, and two Bolt plates are bolted, to strong applicability, different supporting rods 12
It uses.
As shown in Fig. 1, Fig. 6 and Fig. 7, the front side of the comparison mechanism body 4, which is equipped with, tilts locking frame 5, and described inclines
The front side of oblique locking frame 5 is additionally provided with triangle placement plate 6, tilt locking frame 5 and triangle placement plate 6 to Protein Detection during
Reagent bottle plays the role of fixed.
Sliding rail 18 is equipped in the test panel 2 for tilting locking 5 position of frame, it is described to tilt locking frame 5
It is hinged there are two semidefinite arc panel 503 on the rotation snap-gauge 502 including vertical upright bar 501 and rotation snap-gauge 502, it is described partly
The inside for determining arc panel 503 is equipped with anti-skid rubber cushion 504, and the end of the semidefinite arc panel 503 is equipped with and is screwed
Plate 505, is equipped with internal thread hole 506 on the plate 505 that is screwed, vertical upright bar 501 can the sliding in sliding rail 18, from
And adjustable different position, the bolt that two semidefinite arc panels 503 are threadably secured on plate 505 are fixed, it can be to reagent bottle
Locking effect is played, while the bolt being screwed on plate 505 being come loose, reagent bottle can be removed, it is compact-sized and tie
Stable connection between structure, it is easy to operation.
The upper end of the vertical upright bar 501 is equipped with arch sheet 507, and weldering is fixed in the lower surface of 502 upper end of the rotation snap-gauge
It is connected to and tightens bolt 508, the outside of the arch sheet 507 is equipped with matching nut 509, and the end for tightening bolt 508 is fixed
Be connected with it is hand-held turn disk 510, tighten the thread segment that bolt 508 is equipped with and matches nut 509 and be mutually twisted, tighten bolt
508 smooth section can be rotated in arch sheet 507, and after turning to specific angle, matching nut 509 is twisted with bolt 508 is tightened
Tightly, it can be achieved that the rotation and fixation of rotation snap-gauge 502.
It should be added that the rotation snap-gauge 502 mainly consolidates the condenser pipe in protein measurement instrument
It is fixed, while condenser pipe can set angle of inclination, improve practicability.
The lower end of the vertical upright bar 501 is also hinged with check lever 511, and the end of the check lever 511 is connected with housing
Fitting 512, also fixed on the arch sheet 507 of the vertical upright bar 501 to paste angled marking plate 513, check lever 511 and outer
Coupling 512 plays the role of fixed rotation snap-gauge 502, prevents rotation snap-gauge 502 to be affected by gravity and rotates backward, influences to use
Safety, outer Coupling 512 can arbitrarily transfer position on rotation snap-gauge 502, so as to the rotation snap-gauge to different rotational angles
502 are fixed.
It should be added that outer Coupling 512 is cavity sliding panel, and it is simple in structure, it is of low cost, while increasing knot
Structure stability, angle marking plate 513 can identify the rotational angle of rotation snap-gauge 502, to intuitively measure the inclination angle of condenser pipe
Degree is easy to use.
As shown in figure 8, the triangle placement plate 6 includes two pieces of anti-off boards 601 being hinged, two anti-off boards
601 other end also is provided with Bolt plate 602, and the bottom of the anti-off board 601 is fixed with straight trip connecting rod 603, and the straight trip connects
Hinged there are three the spike bar 604 of equidistantly distributed on bar 603, the upper end of the spike bar 604 is equipped with rotary cylinder 605, two pieces
The anti-off board 601 being hinged can be fixed reagent bottle by Bolt plate 602, and spike bar 604 is hinged on straight trip connecting rod 603
On, and by the rotational angle of spike bar 604, can constantly adjust the overall height of triangle placement plate 6, wide adaptability.
Firm bar 606 is equipped between three spike bars 604, the centre of the firm bar 606 is equipped with hinge bar
607, the end of the firm bar 606 is equipped with the cavity fixed cylinder 608 being fixed on spike bar 604, and firm bar 606 is fixed respectively
On three spike bars 604, and firm bar 606 can be rotated around hinge bar 607, increase the stability of spike bar 604.
In conclusion the main characteristic of the invention lies in that：
(1) present invention is multiple functional, by using disintegrating mechanism, vibrating motor, tilts locking frame and triangle placement plate, collection
Crushing wheat takes albumen powder, solution vibration mixing and reagent bottle to be supported in one, compact-sized stabilization, the company of being threadably secured
It connects, so stable connection between structure is adaptable to various sizes of reagent bottle and uses by adjusting position and height, it can be square
Just albumen contrast operation is efficiently completed, it is highly practical.
(2) present invention uses the locking plate of reagent bottle, multi-angle rotary measurement can be carried out to reagent bottle, to suitable for operation
Process uses, and structure member makes to be joined with bolts, at low cost, using simple, facilitates arrangement.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
Claims (10)
1. a kind of wheat Siargos genes compare device with albumen, it is characterised in that：Including experiment pedestal (1), the experimental bench
The upper end of seat (1) is equipped with test panel (2), and the upper surface of the test panel (2) is equipped with disintegrating mechanism (3) and comparison mechanism sheet
Body (4), the front side of the comparison mechanism body (4), which is equipped with, tilts locking frame (5), and the front side for tilting locking frame (5)
It is additionally provided with triangle placement plate (6), the comparison mechanism body (4) includes the locking plate of reagent bottle (401) and is arranged in reagent bottle card
The lower surface of heating mechanism (402) below fixed board (401), the test panel (2) is equipped with cooling fan (7), the experiment
Panel (2) is equipped with ventilation hollow slots (8) on the position of cooling fan (7)；
Four angles of the locking plate of reagent bottle (401) are equipped with elastic rod (9), and the lower end of the elastic rod (9) is equipped with suit
The upper end of cavity cylinder (10), the suit cavity cylinder (10) is equipped with locking cap eaves board (11), fixes and sets on the test panel (2)
Have and is equipped with embedded fixing groove (13), the set with elastic rod (9) matched supporting rod (12), the upper surface of the supporting rod (12)
Dress cavity cylinder (10) is fixedly mounted in embedded fixing groove (13), and the outside of the elastic rod (9) is equipped with torsionspring (14), institute
The lower end for stating torsionspring (14) is fixedly welded on supporting rod (12), outside of the test panel (2) in supporting rod (12)
Position is additionally provided with recessed mounting groove (15), and vibrating motor (16), the vibrating motor are equipped in the recessed mounting groove (15)
(16) it is equipped with the vibration rocking bar (17) being connect with supporting rod (12).
2. a kind of wheat Siargos genes according to claim 1 compare device with albumen, it is characterised in that：It is described to add
Heat engine structure (402) includes heating web plate (4021), displacement mobilizing board (4022) and setting in heating web plate (4021) and displacement
Hot plate (4023) between mobilizing board (4022), four angles of heating web plate (4021) and displacement mobilizing board (4022)
Position is equipped with screw thread clip (4024), and the heating web plate (4021) is equipped with soaking asbestos gauge (4025), institute's rheme
Transposition movable plate (4022) is equipped with the non-slip mat (4026) of several equidistantly distributeds, and the hot plate (4023) is placed on anti-skidding
It pads on (4026).
3. a kind of wheat Siargos genes according to claim 1 compare device with albumen, it is characterised in that：The powder
Broken mechanism (3) includes dnockout cylinder (301) and crushes three leaf knives (302), and the bottom of the dnockout cylinder (301) is equipped with drive motor
(303), three leaf knives (302) of the crushing are fixedly mounted on the swingle of drive motor (303), the dnockout cylinder (301)
Lower end is equipped with discharge port (304), and the outside of the discharge port (304) is equipped with striker plate (305), and the two of the discharge port (304)
Side is equipped with limit cavity plate (306).
4. a kind of wheat Siargos genes according to claim 1 compare device with albumen, it is characterised in that：Described
The test panel (2) for tilting locking frame (5) position is equipped with sliding rail (18), described to tilt locking frame (5) including perpendicular
To upright bar (501) and rotation snap-gauge (502), hinged on the rotation snap-gauge (502) there are two semidefinite arc panels (503), described
The inside of semidefinite arc panel (503) is equipped with anti-skid rubber cushion (504), and the end of the semidefinite arc panel (503) is equipped with
Be screwed plate (505), and internal thread hole (506) is equipped on the plate that is screwed (505).
5. a kind of wheat Siargos genes according to claim 4 compare device with albumen, it is characterised in that：It is described perpendicular
It is equipped with arch sheet (507) to the upper end of upright bar (501), the lower surface of described rotation snap-gauge (502) upper end, which is fixedly welded with, tightens
The outside of bolt (508), the arch sheet (507) is equipped with matching nut (509), and the end for tightening bolt (508) is fixed
It is connected with to hold and turns disk (510).
6. a kind of wheat Siargos genes according to claim 5 compare device with albumen, it is characterised in that：It is described perpendicular
It is also hinged with check lever (511) to the lower end of upright bar (501), the end of the check lever (511) is connected with outer Coupling
(512), also fixed on the arch sheet (507) of the vertical upright bar (501) to paste angled marking plate (513).
7. a kind of wheat Siargos genes according to claim 1 compare device with albumen, it is characterised in that：The examination
The locking plate of agent bottle (401) walks slot (19), the elongated side for walking slot (19) equipped with the elongated of several equidistantly distributeds
It is fixedly welded with and accepts column support (20), the undertaking column support (20) is equipped with spiraled cavity plate (21), the spiraled cavity
It is equipped with rotating clamp (22) in plate (21), also is provided with tightening bolt (508) between the rotating clamp (22) and spiraled cavity plate (21),
And the rotating clamp (22) is connected with reagent bottle clamping piece (23).
8. a kind of wheat Siargos genes according to claim 1 compare device with albumen, it is characterised in that：Described three
Angle placement plate (6) includes two pieces of anti-off boards being hinged (601), and the other end of the anti-off board (601) also is provided with Bolt plate
(602), the bottom of the anti-off board (601) is fixed with straight trip connecting rod (603), and three are hinged on the straight trip connecting rod (603)
The upper end of the spike bar (604) of a equidistantly distributed, the spike bar (604) is equipped with rotary cylinder (605).
9. a kind of wheat Siargos genes according to claim 8 compare device with albumen, it is characterised in that：Three institutes
It states and is equipped with firm bar (606) between spike bar (604), the centre of the firm bar (606) is equipped with hinge bar (607), described
The end of firm bar (606) is equipped with the cavity fixed cylinder (608) being fixed on spike bar (604).
10. a kind of wheat Siargos genes according to claim 1 compare device with albumen, it is characterised in that：It is described logical
The wind shield (24) of several equidistantly distributeds is equipped in wind hollow slots (8), two opposite flanks of the ventilation hollow slots (8) are set
There is mounting hole (25), the both ends of the wind shield (24), which are equipped with, assigns bar (26).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810294008.1A CN108562687B (en) | 2018-03-30 | 2018-03-30 | Wheat Siargos is protein contrast device for gene |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810294008.1A CN108562687B (en) | 2018-03-30 | 2018-03-30 | Wheat Siargos is protein contrast device for gene |
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| CN108562687B CN108562687B (en) | 2021-05-04 |
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| CN108562687B (en) | 2021-05-04 |
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