CN106086166A - Reagent of detection paraffin section DNA mass and preparation method and application - Google Patents
Reagent of detection paraffin section DNA mass and preparation method and application Download PDFInfo
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Abstract
The invention discloses and a kind of detect the reagent of paraffin section DNA mass, its preparation method and application.Described reagent comprises NEB thermal starting buffer, oligonucleotide, primers F, primer R and Taq polymerase etc..The preparation method of described reagent includes: first 10 × NEB thermal starting buffer, oligonucleotide, primers F, primer R and Taq polymerase is mixed, uses ultra-pure water constant volume afterwards.A kind of preparation method detecting paraffin section DNA mass reagent that the present invention provides is simple, difficulty is low, without know-how training and special instruments and equipment, and described reagent is high to the accuracy of detection of DNA, average detection qualification rate reaches more than 90%, it it is more than 3 times of classical way qualification rate (about 30%), the consumption of required DNA can be reduced to 1ng, it is about the 1/200 of classical way consumption, the stability of this reagent is high, when being applied to detect DNA mass, easy to operate, accuracy in detection is high.
Description
Technical field
The present invention relates to technical field of molecular biology, more particularly, to a kind of detection stone for DNA technique field
Wax section DNA mass reagent, and preparation method and application.
Background technology
DNA is the material base of human inheritance, in order to understand fully DNA function in human inheritance and growth course and knot
Structure, the detection of DNA sequence becomes the most important means of researching DNA.Within 1977, Sanger has invented and has had milestone significance
End terminates sequencing, and the same year, A.M.Maxam and W.Gilbert invented chemical degradation method.Sanger method because not only simplicity but also
Quickly, and through follow-up continuous improvement, the main flow of first generation DNA sequencing is become.Along with the continuous innovation of science and technology, the
Generation DNA sequencing technology (Sanger) cannot meet the demand of order-checking, nineteen ninety, and United States Non-Provisional starts the cross-centennial " mankind
Genome plan ", plan lasts 15 years, furnishes funds for 3,000,000,000 dollars, decodes mankind itself's heredity secret, and the mankind have stepped into Post genome
Epoch.Along with the massive demand to DNA information, secondary order-checking is born, and this technology is based on synthesis limit, limit sequencing technologies, generation
There are Roche (454), Illumina (Solexa), ABI (SOLID) in table company.The most basic technology of secondary sequencing technologies is exactly two
For the structure of sequencing library, first library construction is initial carries out broken and fragmentation, primary hand by the genomic DNA of sample to be tested
Section has shearing force, ultrasound wave, nitrogen, enzyme action etc., after obtaining fragmentation DNA, connects plus asymmetric order-checking for convenience
Head, it is necessary to first fragmentation DNA is done end-filling and tail end adds single adenine, in classical library construction flow process end-filling and
It is complete independently that afterbody adds the step of adenine.
Along with developing rapidly of secondary sequencing technologies, this technology is also widely used for genetic mutation, disease pathogenesis,
The plurality of medicals such as Genetic Mechanisms, the medical field such as hereditism.
In the medical field, scientific research personnel need to extract DNA from various different tissue specimens, but flesh tissue specimen is come
Source is limited, so generally clinical sample all uses paraffin embedding mode to process tissue, the amount of DNA causing acquisition the most all can be inclined
Few, DNA damage is big, and degraded is serious, is therefore extremely difficult to normal gene group DNA and carries out quality testing amount (200ng), and in inspection
During survey, DNA damage is serious, is difficult to judge whether the quality of DNA can meet next step needs building storehouse, cuts for paraffin
Sheet sample, the quality determining method of DNA has become as the secondary sequencing technologies of restriction and is applied to the primary factor that clinical sample is analyzed,
Therefore improve the quality determining method of DNA and develop specific quality testing means for paraffin section and become to become and compel
The problem needing to solve of the eyebrows and eyelashes.
Summary of the invention
The main object of the present invention is aiming at above present situation, solves to limit secondary sequencing technologies on clinical sample is analyzed
Bottleneck problem, it is provided that a kind of detect paraffin section DNA mass reagent, and preparation method and application, this agent formulations letter
Single, preparation manipulation is convenient, with low cost, is not required to special installation, and can be the cheapest, and preparation manipulation is simple, and can will replace
Traditional DNA quality inspection steps, reduces sample losses, improves the accuracy of DNA quality testing, reduces DNA quality testing institute
Need sample requirement.
For realizing aforementioned invention purpose, the technical solution used in the present invention includes:
A kind of reagent detecting paraffin section DNA mass, it comprises 10 × NEB thermal starting buffer, oligonucleotide, primer
F, primer R and Taq polymerase.
Among some embodiments, the reagent of described detection paraffin section DNA mass comprises:
10 × NEB thermal starting buffer 2-10 parts by volume,
Concentration is the oligonucleotide solution 0.1-3 parts by volume of 10mM,
Concentration is 10uM primers F 0.1-3 liquor capacity part,
Concentration is 10uM primer R 0.1-3 liquor capacity part,
Taq polymerase 0.1-2.5 liquor capacity part,
Remainder includes ultra-pure water.
Among some embodiments, the reagent of described detection paraffin section DNA mass comprises:
10 × NEB thermal starting buffer 2-10 parts by volume,
Concentration is the oligonucleotide solution 0.1-3 parts by volume of 10mM,
Concentration is 10uM primers F 0.1-3 liquor capacity part,
Concentration is 10uM primer R 0.1-3 liquor capacity part,
Taq polymerase 0.1-2.5 liquor capacity part,
Ultra-pure water 0-20 liquor capacity part.
Among some more preferred embodiment, the reagent of described detection paraffin section DNA mass comprises:
10 × NEB thermal starting buffer 2.5 parts by volume,
Concentration is oligonucleotide solution 0.5 parts by volume of 10mM,
Concentration is 10uM primers F solution 1 parts by volume,
Concentration is 10uM primer R solution 1 parts by volume,
Taq polymerase solution 0.5 parts by volume,
Ultra-pure water 14.5 parts by volume.
Among some more preferred embodiment, described primers F has the nucleotides sequence shown in SEQ ID No.1
Row.
Among some more preferred embodiment, described primer R has the nucleotides sequence shown in SEQ ID No.2
Row.
The present invention proposes a kind of preparation method detecting paraffin section DNA mass reagent and includes: first 10 × NEB heat opened
Dynamic buffer, oligonucleotide, primers F, primer R and Taq polymerase mixing, use ultra-pure water constant volume afterwards.
A kind of method that the invention allows for reagent detecting paraffin section DNA mass, it comprises the following steps: first will
Paraffin section DNA and the reagent mix homogeneously of described detection paraffin section DNA mass, carry out PCR amplification afterwards.
Further, the condition of described PCR amplification includes: react 30-50min under 62 DEG C of-95 DEG C of temperature conditionss.
Concrete, described PCR amplification includes:
First carrying out 35 circulations, each circulation includes: be incubated 30 seconds at 95 DEG C successively, and 95 DEG C are incubated 30 seconds, 62 DEG C of guarantors
Temperature 30 seconds；
Thereafter, being incubated 30 seconds at 72 DEG C successively, 72 DEG C are incubated 5 minutes.
Further, the reagent dosage of described paraffin section DNA and described detection paraffin section DNA mass is than for 5-
15ng:25 μ L.
The invention allows for a kind of test kit detecting paraffin section DNA mass, it includes that above-mentioned detection paraffin is cut
The reagent of sheet DNA mass.
Compared with prior art, the invention have the advantages that
(1) a kind of detection paraffin section DNA mass reagent that the present invention provides, high to the accuracy of detection of DNA, averagely detects
Qualification rate reaches more than 90%, is 3 times of classical way qualification rate (about 30%), and required amount of DNA can be reduced to below 1ng,
It is about the 1/200 of classical way consumption, and the stability of this reagent is high, when being applied to detect DNA mass, easy to operate,
Degree of detection is higher.
(2) a kind of preparation method detecting paraffin section DNA mass reagent that the present invention provides, material therefor is easy to get, valency
Lattice are cheap, beneficially popularization and application, and above-mentioned raw materials only need to be dissolved in by the detection paraffin section DNA mass reagent of the preparation present invention
Ultra-pure water；Preparation is simple, and difficulty is low, it is not necessary to know-how training and special instruments and equipment.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Some embodiments described in invention, for those of ordinary skill in the art, on the premise of not paying creative work,
Other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the consumption of DNA and the graph of a relation detecting DNA qualification rate in embodiment of the present invention 1-4 and matched group.
Detailed description of the invention
Hereafter technical scheme will be made the most detailed explanation.It is understood, however, that at model of the present invention
In enclosing, can between above-mentioned each technical characteristic and each technical characteristic specifically described in below (eg embodiment) of the present invention
It is combined with each other, thus constitutes new or preferred technical scheme.As space is limited, the most tired at this state.
Before example is described, it is necessary to provide some remarks to illustrate:
Use different manufacturers, the reagent of different batches can cause the difference of experimental result, belong to normal phenomenon.Carry out little
Sweeping experiment room, for ensureing the repeatability between parallel laboratory test, it is proposed that after reagent preparation, fully mixing subpackage, to ensure every time
The homogeneity of experiment reagent.
The invention discloses a kind of reagent detecting paraffin section DNA mass, its comprise 10 × NEB thermal starting buffer,
Oligonucleotide, primers F, primer R and Taq polymerase.
Among some embodiments, the reagent of described detection paraffin section DNA mass, it comprises:
10 × NEB thermal starting buffer 2-10 parts by volume,
Concentration is the oligonucleotide solution 0.1-3 parts by volume of 10mM,
Concentration is 10uM primers F solution 0.1-3 parts by volume,
Concentration is 10uM primer R solution 0.1-3 parts by volume,
Taq polymerase 0.1-2.5 liquor capacity part,
Remainder includes ultra-pure water.
Among some embodiments, the reagent of described detection paraffin section DNA mass, it comprises:
10 × NEB thermal starting buffer 2-10 parts by volume,
Concentration is the oligonucleotide solution 0.1-3 parts by volume of 10mM,
Concentration is 10uM primers F solution 0.1-3 parts by volume,
Concentration is 10uM primer R solution 0.1-3 parts by volume,
Taq polymerase 0.1-2.5 liquor capacity part,
Ultra-pure water 0-20 parts by volume.
Among some more preferred embodiment, the reagent of described detection paraffin section DNA mass, it comprises:
10 × NEB thermal starting buffer 2.5 parts by volume,
Concentration is oligonucleotide solution 0.5 parts by volume of 10mM,
Concentration is 10uM primers F solution 1 parts by volume,
Concentration is 10uM primer R solution 1 parts by volume,
Taq polymerase solution 0.5 parts by volume,
Ultra-pure water 14.5 parts by volume.
Among some more preferred embodiment, described primers F has the nucleotides sequence shown in SEQ ID No.1
Row, the most following sequence AATCGGGCTG.
Among some more preferred embodiment, described primer R has the nucleotides sequence shown in SEQ ID No.2
Row, the most following sequence GAAACGGGTG.
The present invention proposes a kind of preparation method detecting paraffin section DNA mass reagent and includes: first 10 × NEB heat opened
Dynamic buffer, oligonucleotide, primers F, primer R and Taq polymerase mixing, use ultra-pure water constant volume afterwards.
A kind of method that the invention allows for reagent detecting paraffin section DNA mass, it comprises the following steps: first will
Paraffin section DNA and the reagent mix homogeneously of described detection paraffin section DNA mass, carry out PCR amplification afterwards.
Present invention also offers a kind of method detecting paraffin section DNA mass reagent, comprise the following steps:
A. take 1 0.2ml PCR pipe, paraffin section DNA (1-10) is accurately settled to 5 μ L and adds in this PCR pipe, more accurate
Really add quickly detection paraffin section DNA mass reagent 25 μ L.
B. use suction pipette head that mixture in this pipe is uniformly blown and beaten mixing more than 10 times.
C. the mixture PCR pipe that will be equipped with mix homogeneously is positioned in PCR instrument.
D. correct response procedures is run.
Further, the condition of described PCR amplification includes: react 30-50min under 62 DEG C of-95 DEG C of temperature conditionss.
Concrete, described PCR amplification includes:
First carrying out 35 circulations, each circulation includes: be incubated 30 seconds at 95 DEG C successively, and 95 DEG C are incubated 30 seconds, 62 DEG C of guarantors
Temperature 30 seconds；
Thereafter, being incubated 30 seconds at 72 DEG C successively, 72 DEG C are incubated 5 minutes.
Further, the reagent dosage ratio of described paraffin section DNA and described detection paraffin section DNA mass be 5～
15ng:25uL.
Further, by products therefrom, it is directly added into electrophoresis loading system, after mixing, is positioned in electrophresis apparatus and carries out electricity
Swimming, finally uses imager to carry out detection of taking pictures, and it is the most qualified to add up product.
Further, described electrophoresis loading system, its concrete composition is sample-loading buffer 5ul, fully mixes.
Further, the deposition condition that described electrophresis apparatus is carried out is 2% agarose gel, 100 volts of voltages, the time
1 hour.
The invention allows for a kind of test kit detecting paraffin section DNA mass, it includes that above-mentioned detection paraffin is cut
The reagent of sheet DNA mass.
Below in conjunction with drawings and Examples, the technology of the present invention is further explained.
Embodiment 1
1. reagent preparation:
Pipettor is used accurately to measure 10 × NEB thermal starting buffer 2.5 μ L, 10mM oligonucleotide 0.5 μ L, 10uM primer
F 1 μ L, 10uM primer R 1 μ L, Taq polymerase 0.5 μ L, and use ultra-pure water to be settled to 20 μ L.
2. experimental implementation process:
A. take 8 0.2ml PCR pipe, paraffin section DNA 1ng (8 each 1ng of sample) is accurately settled to 5 μ L additions right
In answering PCR pipe, add quickly detection paraffin section DNA mass reagent 25 μ L.
B. use suction pipette head that mixture in this pipe is uniformly blown and beaten mixing more than 10 times.
C. the mixture PCR pipe that will be equipped with mix homogeneously is positioned in PCR instrument.
D. correct response procedures is run.
Response procedures is: 95 DEG C 30 seconds, 95 DEG C 30 seconds, 62 DEG C 30 seconds, 72 DEG C 30 seconds (35 circulations), 72 DEG C 5 minutes.
3. the method for inspection:
Step (2) products therefrom is directly added into electrophoresis sample-loading buffer 5 μ L, and adds after using pipettor fully to mix
To 2% agarose gel, then it is positioned in electrophoretic buffer, and opens electrophresis apparatus 100 volts of voltages of selection, electrophoresis 1 hour
After, afterwards gel is taken out from electrophresis apparatus, be positioned on imager, take pictures and record result, be not less than according to maximum band
500 base pairs are standard, respectively statistics qualification rate and detection qualification rate.
With reference to Fig. 1, finally recording qualification rate is: 75%, detects qualification rate 100%.
Embodiment 2
1. reagent preparation:
Pipettor is used accurately to measure 10 × NEB thermal starting buffer 2.5 μ L, 10mM oligonucleotide 0.5 μ L, 10uM primer
F 1 μ L, 10uM primer R 1 μ L, Taq polymerase 0.5 μ L, and use ultra-pure water to be settled to 20 μ L.
2. experimental implementation process:
A. take 8 0.2ml PCR pipe, paraffin section DNA 5ng (8 each 5ng of sample) is accurately settled to 5 μ L additions right
In answering PCR pipe, add quickly detection paraffin section DNA mass reagent 25 μ L.
B. use suction pipette head that mixture in this pipe is uniformly blown and beaten mixing more than 10 times.
C. the mixture PCR pipe that will be equipped with mix homogeneously is positioned in PCR instrument.
D. correct response procedures is run.
Response procedures is: 95 DEG C 30 seconds, 95 DEG C 30 seconds, 62 DEG C 30 seconds, 72 DEG C 30 seconds (35 circulations), 72 DEG C 5 minutes.
3. the method for inspection:
Step (2) products therefrom is directly added into electrophoresis sample-loading buffer 5 μ L, and adds after using pipettor fully to mix
To 2% agarose gel, then it is positioned in electrophoretic buffer, and opens electrophresis apparatus 100 volts of voltages of selection, electrophoresis 1 hour
After, afterwards gel is taken out from electrophresis apparatus, be positioned on imager, take pictures and record result, be not less than according to maximum band
500 base pairs are standard, respectively statistics qualification rate and detection qualification rate.
With reference to Fig. 1, finally recording qualification rate is: 87.5%, detects qualification rate 100%.
Embodiment 3
1. reagent preparation:
Pipettor is used accurately to measure 10 × NEB thermal starting buffer 2.5 μ L, 10mM oligonucleotide 0.5 μ L, 10uM primer
F 1 μ L, 10uM primer R 1 μ L, Taq polymerase 0.5 μ L, and use ultra-pure water to be settled to 20 μ L.
2. experimental implementation process:
A. take 8 0.2ml PCR pipe, paraffin section DNA 10ng (8 each 10ng of sample) is accurately settled to 5 μ L and adds
In corresponding PCR pipe, add quickly detection paraffin section DNA mass reagent 25 μ L.
B. use suction pipette head that mixture in this pipe is uniformly blown and beaten mixing more than 10 times.
C. the mixture PCR pipe that will be equipped with mix homogeneously is positioned in PCR instrument.
D. correct response procedures is run.
Response procedures is: 95 DEG C 30 seconds, 95 DEG C 30 seconds, 62 DEG C 30 seconds, 72 DEG C 30 seconds (35 circulations), 72 DEG C 5 minutes.
3. the method for inspection:
Step (2) products therefrom is directly added into electrophoresis sample-loading buffer 5 μ L, and adds after using pipettor fully to mix
To 2% agarose gel, then it is positioned in electrophoretic buffer, and opens electrophresis apparatus 100 volts of voltages of selection, electrophoresis 1 hour
After, afterwards gel is taken out from electrophresis apparatus, be positioned on imager, take pictures and record result, be not less than according to maximum band
500 base pairs are standard, respectively statistics qualification rate and detection qualification rate.
With reference to Fig. 1, finally recording qualification rate is: 100%, detects qualification rate 87.5%.
Embodiment 4
1. reagent preparation:
Pipettor is used accurately to measure 10 × NEB thermal starting buffer 2.5 μ L, 10mM oligonucleotide 0.5 μ L, 10uM primer
F 1 μ L, 10uM primer R 1 μ L, Taq polymerase 0.5 μ L, and use ultra-pure water to be settled to 20 μ L.
2. experimental implementation process:
A. take 8 0.2ml PCR pipe, paraffin section DNA 1ng (8 each 1ng of sample) is accurately settled to 5 μ L additions right
In answering PCR pipe, add quickly detection paraffin section DNA mass reagent 25 μ L.
B. use suction pipette head that mixture in this pipe is uniformly blown and beaten mixing more than 10 times.
C. the mixture PCR pipe that will be equipped with mix homogeneously is positioned in PCR instrument.
D. correct response procedures is run.
Response procedures is: 95 DEG C 30 seconds, 95 DEG C 30 seconds, 62 DEG C 30 seconds, 72 DEG C 30 seconds (35 circulations), 72 DEG C 5 minutes.
3. the method for inspection:
Step (2) products therefrom is directly added into electrophoresis sample-loading buffer 5 μ L, and adds after using pipettor fully to mix
To 2% agarose gel, then it is positioned in electrophoretic buffer, and opens electrophresis apparatus 100 volts of voltages of selection, electrophoresis 1 hour
After, afterwards gel is taken out from electrophresis apparatus, be positioned on imager, take pictures and record result, be not less than according to maximum band
500 base pairs are standard, respectively statistics qualification rate and detection qualification rate.
With reference to Fig. 1, finally recording qualification rate is: 62.5%, detects qualification rate 100%.
Matched group embodiment
1. reagent preparation:
Pipettor is accurately used to measure Qubit DNA fluorescent quantitation reagent 1 μ L, Qubit DNA fluorescent quantitation buffer 199
μ L, and mix both, it is configured to working solution.
2. experimental implementation process:
A. take the high quantity tube thoroughly of 1 0.5ml, paraffin section DNA is taken 1 μ L, adds to working solution.
The most fully mix mixture, and lucifuge room temperature placed 2 minutes,
C. mixed liquor is positioned in Qubit instrument, chooses highly sensitive quant program.
D. proceed by DNA to measure, and record.
3. the method for inspection:
Step (2) products therefrom is directly added into electrophoresis sample-loading buffer 5 μ L, and adds after using pipettor fully to mix
To 2% agarose gel, then it is positioned in electrophoretic buffer, and opens electrophresis apparatus 100 volts of voltages of selection, electrophoresis 1 hour
After, afterwards gel is taken out from electrophresis apparatus, be positioned on imager, take pictures and record result, be not less than according to maximum band
500 base pairs are standard, respectively statistics qualification rate and detection qualification rate.
With reference to Fig. 1, finally recording qualification rate is: 25%, detects qualification rate 37.5%.
The classical reagent that the quickly detection paraffin section DNA mass reagent that the present invention uses uses than matched group has low
The feature that DNA consumes and detection qualification rate is high and stable, and in the case of usage amount identical (1ng), also have identical detection
Qualification rate, it is seen that the reagent stability of the present invention is preferable.
Should be appreciated that above-described embodiment is only technology design and the feature of the explanation present invention, its object is to allow and be familiar with this
The personage of item technology will appreciate that present disclosure and implements according to this, can not limit the scope of the invention with this.All
The equivalence change made according to spirit of the invention or modification, all should contain within protection scope of the present invention.
Claims (10)
1. the reagent detecting paraffin section DNA mass, it is characterised in that comprise 10 × NEB thermal starting buffer, oligonucleoside
Acid, primers F, primer R and Taq polymerase.
The reagent of detection paraffin section DNA mass the most according to claim 1, it is characterised in that comprise:
10 × NEB thermal starting buffer 2-10 parts by volume,
Concentration is the oligonucleotide solution 0.1-3 parts by volume of 10mM,
Concentration is 10uM primers F solution 0.1-3 parts by volume,
Concentration is 10uM primer solution R 0.1-3 parts by volume,
Taq polymerase solution 0.1-2.5 parts by volume,
Remainder includes ultra-pure water.
The reagent of detection paraffin section DNA mass the most according to claim 2, it is characterised in that comprise:
10 × NEB thermal starting buffer 2-10 parts by volume,
Concentration is the oligonucleotide solution 0.1-3 parts by volume of 10mM,
Concentration is 10uM primers F solution 0.1-3 parts by volume,
Concentration is 10uM primer R solution 0.1-3 parts by volume,
Taq polymerase solution 0.1-2.5 parts by volume,
Ultra-pure water 0-20 parts by volume.
The reagent of detection paraffin section DNA mass the most according to claim 3, it is characterised in that comprise:
10 × NEB thermal starting buffer 2.5 parts by volume,
Concentration is oligonucleotide solution 0.5 parts by volume of 10mM,
Concentration is 10uM primers F solution 1 parts by volume,
Concentration is 10uM primer R solution 1 parts by volume,
Taq polymerase solution 0.5 parts by volume,
Ultra-pure water 14.5 parts by volume.
5. according to the reagent of the detection paraffin section DNA mass according to any one of claim 1-4, it is characterised in that described
Primers F has the nucleotide sequence shown in SEQ ID No.1；And/or, described primer R has the nucleoside shown in SEQ ID No.2
Acid sequence.
6. as according to any one of claim 1-5, detect the preparation method of paraffin section DNA mass reagent, it is characterised in that bag
Include: first 10 × NEB thermal starting buffer, oligonucleotide, primers F, primer R and Taq polymerase are mixed, fixed with ultra-pure water afterwards
Hold.
7. the method detecting paraffin section DNA mass, it is characterised in that including: first by paraffin section DNA and claim
The reagent mix homogeneously of the detection paraffin section DNA mass according to any one of 1-5, carries out PCR amplification afterwards.
The method of the reagent of detection paraffin section DNA mass the most according to claim 7, it is characterised in that described PCR expands
The condition increased includes: react 30-50min under 62 DEG C of-95 DEG C of temperature conditionss,
And/or, the reagent dosage of described paraffin section DNA and described detection paraffin section DNA mass is than for 5-15ng:25 μ μ L.
The method of the reagent of detection paraffin section DNA mass the most according to claim 8, it is characterised in that described PCR expands
Increasing includes:
First carrying out 35 circulations, each circulation includes: be incubated 30 seconds at 95 DEG C successively, and 95 DEG C are incubated 30 seconds, 62 DEG C of insulations 30
Second；
Thereafter, being incubated 30 seconds at 72 DEG C successively, 72 DEG C are incubated 5 minutes.
10. a test kit, it is characterised in that comprise the detection paraffin section DNA mass according to any one of claim 1-5
Reagent.
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| CN108103168A (en) * | 2016-11-23 | 2018-06-01 | 安诺优达基因科技(北京)有限公司 | The appraisal procedure of DNA mass in a kind of FFPE samples |
| CN116773291A (en) * | 2023-03-31 | 2023-09-19 | 艾普拜生物科技(苏州)有限公司 | Paraffin slice DNA pretreatment reagent and method |
| CN116773291B (en) * | 2023-03-31 | 2024-04-19 | 艾普拜生物科技(苏州)有限公司 | Paraffin slice DNA pretreatment reagent and method |
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