CN105816396B - For the plant composition of whitening, plant extraction liquid and preparation method thereof - Google Patents
For the plant composition of whitening, plant extraction liquid and preparation method thereof Download PDFInfo
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- CN105816396B CN105816396B CN201610371046.3A CN201610371046A CN105816396B CN 105816396 B CN105816396 B CN 105816396B CN 201610371046 A CN201610371046 A CN 201610371046A CN 105816396 B CN105816396 B CN 105816396B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The plant composition that the present invention provides a kind of for whitening, plant extraction liquid and preparation method thereof；The plant composition includes each component of following weight percentage：Tealeaves 30~60%, scutellariae,radix 15~20%, apple 5~20%, cucumber 5~10%, white jade orchid 5~20%.The plant extraction liquid is obtained by the plant composition for whitening for raw material extraction.The preparation method of the plant extraction liquid includes：Extraction step, microfiltration step, ultrafiltration step, concentration step and preparation steps.The method of the present invention prepare plant extraction liquid stability is good, active constituent is more, for having extraordinary white-skinned face function in cosmetics.
Description
Technical field
The present invention relates to cosmetic fields, are related to a kind of plant composition, plant extraction liquid and its preparation for whitening
Method.
Background technology
With environmental disruption, the irregular shadow with the irradiation of depletion of the ozone layer ultraviolet light of the excessive rhythm of life of pollutants in air
It rings, it is that women never stopped demand, and natural harmless cosmetics are even more the object that people pursue to protect skin and whitening.
Tea polyphenols in tealeaves can stop ultraviolet light, remove the free radical that ultraviolet light generates, and inhibit fat in skin mitochondria
Oxygenase and lipid peroxidation, to have anti-aging effects, experiment, which shows tea polyphenols also, to be had antiallergic action and resists
Cutaneous anaphylaxis acts on.
There is inhibiting effect to dermatophyte containing flavone compounds such as scutelloside, baicaleins in radix scutellariae, inoxidizability is strong,
And experiments have shown that there is anti-inflammatory and antiallergic action, to the sufficient swollen, adjuvant-induced arthritis of rat and passive cutaneous anaphylaxis
There is inhibiting effect.
Apple is one of most common fruit, soluble big containing nutrition, is easily absorbed by the body, wherein malic acid
Useless extra cutin, reinforcement skin are metabolized always for mild removal；The constituent of glycine collagen and elastin laminin；Half Guang ammonia
Acid is tyrosinase inhibitor, there is whitening function；Aspartic acid is the required essential amino acid of cell metabolism；Quercitrin
The antioxidant such as element, citric acid, carrotene, the free radical in scavenger-cell；Phloretin and phloridzin in apple skin are junket
Propylhomoserin enzyme inhibitor；There are elements, the human bodies such as copper, iodine, manganese, zinc, potassium such as to lack these elements in apple, skin will be done
It is dry, easy to crack, very itch.
Containing abundant vitamin C, vitamin E, carrotene in cucumber, free radical can be resisted, vitamin C can be also
Melanin in raw hide skin.
Flos micheliae Albae can inhibit skin injury caused by UVB according to the literature, and Flos micheliae Albae flower extract is to tyrosinase
Inhibiting effect be IC50=13.6%.
More whitening extracting solution raw material is mostly single plant material on the market at present, and action pathway is single, and carries
Take liquid color deep, thermal stability is poor, and active constituent is few.
Invention content
For the defects in the prior art, the plant composition that the object of the present invention is to provide a kind of for whitening, plant
Extracting solution and preparation method thereof.The present invention by will include tealeaves, Radix Astragali, apple, cucumber and white jade orchid plant composition
Mixing extraction is carried out, the plant extraction liquid of acquisition is of light color, thermostabilization is good, active constituent is more, has good white-skinned face function.
The purpose of the present invention is what is be achieved through the following technical solutions：
The present invention provides a kind of plant composition for whitening, the composition includes following weight percentage
Each component：
The apple, cucumber and white jade orchid are fresh weight, and tealeaves and radix scutellariae are dry weight.
The present invention also provides a kind of plant extraction liquid for whitening, the extracting solution can be by the side that includes the following steps
Method is prepared：Using the above-mentioned plant composition for whitening as raw material, extracts and obtain.
The present invention also provides a kind of preparation methods of the plant extraction liquid for whitening, and the preparation method includes following
Step：
Extraction step：The tealeaves weighed, scutellariae,radix, apple, cucumber and white jade orchid composition mixed raw material are impregnated into 2h, adopted
With stirring extraction 4h at 45 DEG C of water extraction, crude extract is obtained；
Microfiltration step：By crude extract successively through 1 μm of ceramic membrane and 0.2 μm of ceramic membrane filter, coarse filtration liquid is obtained；
Ultrafiltration step：By coarse filtration liquid through the film ultrafiltration that molecular cut off is 1500D, filtrate is obtained；
Concentration step：By filtrate through the film nanofiltration that molecular cut off is 150D, concentrate is obtained；
Preparation steps：Water and 1 is added in concentrate, 3 butanediols mix up to the plant extraction liquid.
Preferably, in extraction step, in the water extraction, the weight ratio of mixed raw material and aqueous solution is 1:8.
Preferably, the aqueous solution is the aqueous solution for including 1wt% cellulases and 1wt% pectases.
Preferably, in microfiltration step, the operating pressure of the filtering is 10~15bar, temperature≤45 DEG C.
Preferably, in ultrafiltration step, the operating pressure of the filtering is 3~6bar, temperature≤45 DEG C.
Preferably, in ultrafiltration step, in the ultra-filtration process, when phegma remains about 30%, the water for supplementing equivalent continues to surpass
It filters to the amount of diffusate and is equal to starting extraction liquid measure.
Preferably, in concentration step, the operating pressure of the nanofiltration is 10~15bar, temperature≤45 DEG C；The concentrate
The weight ratio of middle extract and aqueous solution is 1:1.6.The extract is the substance extracted from mixed raw material.
Preferably, extract and 1 in the plant extraction liquid obtained, the weight ratio of 3 butanediol aqueous solutions is 1:16；It plants
1,3 butanediol mass concentrations are 20% in object extracting solution.The 1,3 butanediol aqueous solution is that 1,3 butanediols are mixed with what water was formed
Close solution.Concentrate is diluted 10 times by the step of preparation by adding 1,3 butanediols and water.
The present invention also provides a kind of applications for the plant extraction liquid of whitening in cosmetics.
Preferably, the cosmetics include water, lotion and cream.
Preferably, additive amount of the plant extraction liquid in lotion is 5wt%.
The dominant mechanism of whitening includes scavenging activated oxygen, inhibits tyrosinase, reduction melanin intermediates, adjusts black
The Proliferation, Differentiation of plain cell promotes cuticula to fall off, and traditional whitening product active ingredient mechanism of action is more single, and plant
The most high temperature extraction of extracting solution, extraction efficiency is low, active material destructible, and color is relatively deep or is easy discoloration, and stability is poor.And this
Invention selects raw material from a variety of lightening mechanisms, and selects the plant for having synergistic function, super using enzymolysis and extraction-micro-filtration-
The technique of filter-film concentration-preparation, improves the yield of active material, increases the stability of product, practicability and environmental friendly property.
Compared with prior art, the present invention has following advantageous effect：
1, by selecting technique and material combination, the activity and yield of active ingredient are improved.
2, (activated carbon decolorizing can cause flavones ingredient a large amount of for the reservation of increase small molecule ingredient such as Polyphenols, flavonoids
Loss).
3, enhance the stability of extracting solution, reduce discoloration of the extracting solution in long-term placement process.
4, the salt component in extracting solution is removed, avoids extracting solution from being added in cosmetic formulations and generates viscosity reduction, demulsification etc. now
As.
5, without using ethyl alcohol, resin etc. in preparation engineering, Environmental Safety is cost-effective.
6, the plant extraction liquid prepared is for having extraordinary white-skinned face function in cosmetics.
Specific implementation mode
With reference to specific embodiment, the present invention is described in detail.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection domain.
Embodiment 1
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 40%, scutellariae,radix 20%, apple 10%, cucumber 10%, white jade orchid 20%.The apple, cucumber and
White jade orchid is fresh weight, and tealeaves and radix scutellariae are dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
Tealeaves, radix scutellariae, apple, cucumber and white jade orchid are impregnated into 2h, 8 times of water add 1% cellulase and 1% pectase 45
DEG C stirring extraction 4h；1 μm of ceramic membrane filter of micro-filtration, then with 0.2 μm of ceramic membrane filter, pressure 10bar；UF retention point
The film that son amount is 1500D, operating pressure 5bar, ultrafiltration it is surplus to phegma about 30% when, the water for supplementing equivalent continues ultrafiltration to oozing
The amount for going out liquid is equal to starting extraction liquid measure；The film that concentration is 150D with molecular cut off, operating pressure 10bar, temperature holding≤
45 DEG C, nanofiltration to concentrate is equivalent to 0.5g/ml, and (weight ratio of extract and aqueous solution is 1 i.e. in concentrate:1.6)；Finally
1,3 butanediols and water is added, concentrate is diluted 10 times, is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1,3 butanediols
The weight ratio of aqueous solution is 1:16), 1,3 butanediol mass concentrations be 20%, as this programme extracting solution (ceramic membrane filter,
The temperature of ultrafiltration keeps≤45 DEG C).
Embodiment 2
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 60%, scutellariae,radix 15%, apple 10%, cucumber 5%, white jade orchid 10%.The apple, cucumber and
White jade orchid is fresh weight, and tealeaves and radix scutellariae are dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
Tealeaves, radix scutellariae, apple, cucumber and white jade orchid are impregnated into 2h, 8 times of water add 1% cellulase and 1% pectase 45
DEG C stirring extraction 4h；1 μm of ceramic membrane filter of micro-filtration, then with 0.2 μm of ceramic membrane filter, pressure 12bar；UF retention point
The film that son amount is 1500D, operating pressure 3bar, ultrafiltration it is surplus to phegma about 30% when, the water for supplementing equivalent continues ultrafiltration to oozing
The amount for going out liquid is equal to starting extraction liquid measure；The film that concentration is 150D with molecular cut off, operating pressure 12bar, temperature holding≤
45 DEG C, nanofiltration to concentrate is equivalent to 0.5g/ml, and (weight ratio of extract and aqueous solution is 1 i.e. in concentrate:1.6)；Finally
1,3 butanediols and water is added, concentrate is diluted 10 times, is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1,3 butanediols
The weight ratio of aqueous solution is 1:16), 1,3 butanediol mass concentrations be 20%, as this programme extracting solution (ceramic membrane filter,
The temperature of ultrafiltration keeps≤45 DEG C).
Embodiment 3
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 50%, scutellariae,radix 20%, apple 10%, cucumber 5%, white jade orchid 15%.The apple, cucumber and
White jade orchid is fresh weight, and tealeaves and radix scutellariae are dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
Tealeaves, radix scutellariae, apple, cucumber and white jade orchid are impregnated into 2h, 8 times of water add 1% cellulase and 1% pectase 45
DEG C stirring extraction 4h；1 μm of ceramic membrane filter of micro-filtration, then with 0.2 μm of ceramic membrane filter, pressure 15bar；UF retention point
The film that son amount is 1500D, operating pressure 6bar, ultrafiltration it is surplus to phegma about 30% when, the water for supplementing equivalent continues ultrafiltration to oozing
The amount for going out liquid is equal to starting extraction liquid measure；The film that concentration is 150D with molecular cut off, operating pressure 15bar, temperature holding≤
45 DEG C, nanofiltration to concentrate is equivalent to 0.5g/ml, and (weight ratio of extract and aqueous solution is 1 i.e. in concentrate:1.6)；Finally
1,3 butanediols and water is added, concentrate is diluted 10 times, is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1,3 butanediols
The weight ratio of aqueous solution is 1:16), 1,3 butanediol mass concentrations be 20%, as this programme extracting solution (ceramic membrane filter,
The temperature of ultrafiltration keeps≤45 DEG C).
Comparative example 1
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 40%, scutellariae,radix 20%, apple 10%, cucumber 10%, white jade orchid 20%.The apple, cucumber and
White jade orchid is fresh weight, and tealeaves and radix scutellariae are dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
Tealeaves, radix scutellariae, apple, cucumber and white jade orchid are impregnated into 2h, 8 times of water, 60 DEG C of extraction 4h；Extracting solution is concentrated into ratio
1.4 are weighed, adds 95 ° of ethyl alcohol to 80% alcohol precipitation, refrigeration is for 24 hours；Filtering, filtrate are concentrated into proportion 1.3, add ethyl alcohol to 90 ° of alcohol precipitations, refrigeration
24h；Filtering, filtrate are concentrated into proportion 1.2, refrigerate 12h；Filtering, filtrate add water to crude drug 0.5g/ml, and refrigeration is for 24 hours；Filtering, filter
Liquid adds 200 mesh activated carbon, 80 DEG C of 0.5h, and filtering adds 1,3 butanediols and water, is made into and is equivalent to crude drug 0.05g/ml, 1,3 butanediol
A concentration of 20% to get.
Comparative example 1 '
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 40%, scutellariae,radix 20%, apple 10%, cucumber 10%, white jade orchid 20%.The apple, cucumber and
White jade orchid is fresh weight, and tealeaves and radix scutellariae are dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
Tealeaves, radix scutellariae, apple, cucumber and white jade orchid are impregnated into 2h, 4h is boiled in 8 times of boilings；0.3 ‰ chitosan is added to wad a quilt with cotton
It is solidifying, it is slowly stirred 2h, is filtered, filtrate aging 7 days, filtering is concentrated into and is equivalent to 0.5g/ml, adds 1,3 butanediols and water, be made into
Be equivalent to crude drug 0.05g/ml, 1,3 butanediol concentration be 20% to get.
Comparative example 2
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 40%, scutellariae,radix 20%, apple 40%.The apple is fresh weight, and tealeaves and radix scutellariae are dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
Tealeaves, radix scutellariae, apple are impregnated into 2h, 8 times of water add 45 DEG C of stirring extraction 4h of 1% cellulase and 1% pectase；It is micro-
1 μm of ceramic membrane filter is filtered, then with 0.2 μm of ceramic membrane filter, pressure 12bar；UF retention molecular weight is 1500D's
Film, operating pressure 3bar, when ultrafiltration to phegma remains about 30%, the amount for supplementing water continuation ultrafiltration to the diffusate of equivalent is equal to
Begin extraction liquid measure；The film that concentration is 150D with molecular cut off, operating pressure 12bar, temperature are kept for≤45 DEG C, nanofiltration is to dense
Contracting liquid is equivalent to 0.5g/ml, and (weight ratio of extract and aqueous solution is 1 i.e. in concentrate:1.6)；It is eventually adding 1,3 butanediols
Concentrate is diluted 10 times with water, is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1, the weight ratio of 3 butanediol aqueous solutions
It is 1:16), 1,3 butanediol mass concentrations be 20%, as this programme extracting solution (ceramic membrane filter, ultrafiltration temperature protect
Hold≤45 DEG C).
Comparative example 2 '
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 40%, scutellariae,radix 20%, apple 40%.The apple is fresh weight, and tealeaves and radix scutellariae are dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
Tealeaves, radix scutellariae, apple are impregnated into 2h, 4h is boiled in 8 times of boilings；The flocculate with chitosan for adding 0.3 ‰ is slowly stirred 2h, mistake
Filter, filtrate aging 7 days, filtering are concentrated into and are equivalent to 0.5g/ml, add 1,3 butanediols and water, be made into and be equivalent to crude drug 0.05g/
Ml, 1,3 butanediol concentration be 20% to get.
Comparative example 3
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 100%.The tealeaves is dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
By infusion of tea 2h, 8 times of water add 45 DEG C of stirring extraction 4h of 1% cellulase and 1% pectase；Micro-filtration is made pottery with 1 μm
Porcelain membrane filtration, then with 0.2 μm of ceramic membrane filter, pressure 12bar；UF retention molecular weight is the film of 1500D, operating pressure
3bar, when ultrafiltration to phegma remains about 30%, the water for supplementing equivalent continues ultrafiltration to the amount of diffusate equal to starting extraction liquid measure；
The film that concentration is 150D with molecular cut off, operating pressure 12bar, temperature are kept for≤45 DEG C, nanofiltration to concentrate is equivalent to
(weight ratio of extract and aqueous solution is 1 to 0.5g/ml i.e. in concentrate:1.6)；Being eventually adding 1,3 butanediols and water will concentrate
Liquid dilutes 10 times, is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1, the weight ratios of 3 butanediol aqueous solutions are 1:16), 1,
3 butanediol mass concentrations be 20%, as this programme extracting solution (ceramic membrane filter, ultrafiltration temperature keep≤45 DEG C).
Comparative example 3 '
The present embodiment is related to a kind of plant composition with whitening function, and the composition is contained by following weight percent
The each component of amount：Tealeaves 100%.The tealeaves is dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, by above-mentioned plant composition as raw material extract and
.
The preparation method of the plant extraction liquid is as follows：
The present embodiment is related to a kind of plant extract liquid mixture with whitening function, including following weight percent content
Each component：Tealeaves 100%.
By infusion of tea 2h, 4h is boiled in 8 times of boilings；The flocculate with chitosan for adding 0.3 ‰ is slowly stirred 2h, filtering, filtrate aging
7 days, filtering was concentrated into and is equivalent to 0.5g/ml, add 1,3 butanediols and water, is made into and is equivalent to crude drug 0.05g/ml, 1,3 butanediol
A concentration of 20% to get.
Compliance test result test
1, the results are shown in Table 1 for the stability experiment of the embodiment of the present invention and comparative example.
Table 1
2, effect of the invention experiment
2.1 antioxidation in vitro experiments-DPPH radicals scavengings experiment
Principle：DPPH methods were suggested in 1958, were widely used in the oxidation resistance of quantitative determination Biosample and food.
This method is that have single electron according to DPPH free radicals, and it is in the characteristic of purple to have the last one absorption, alcoholic solution at 517nm.When have from
In the presence of base scavenger, its absorption is set to fade away due to being matched with its single electron, the electricity that fading extent receives with it
Subnumber amount is at quantitative relationship, thus available spectrophotometer carries out quick quantitative analysis.
Experiment flow：
(1) DPPH solution is configured：20mgDPPH accurately is weighed, with absolute ethyl alcohol constant volume in 250ml volumetric flasks, is obtained dense
Degree is the DPPH solution of 2.0 × 10-4mol/L, is kept in dark place spare.
(2) it takes 2ml sample liquids in tool plug test tube respectively, the DPPH solution that 2ml is configured is added, be uniformly mixed, in 25
In DEG C water-bath after closed standing 30min, the cuvette that optical path is 1cm is poured into, goes out to measure its absorbance A i in 517nm, surveys simultaneously
2ml samples add absorbance A j and 2ml the DPPH solution of 2ml ethyl alcohol to add the absorbance As 0 of 2ml redistilled waters.Often survey one
Used in amounts will survey three panel datas.Sample liquid is using the extracting solution in embodiment and comparative example.
(3) clearance rate of DPPH=[1 one (Ai-Aj)/A0] * 100%
Experimental result is as shown in table 2：
Table 2
2.2 Tyrosinase inhibition assays
(1) configuration phosphate buffer (pbs) (Ph=6.8) takes KH2PO46.80g, NaOH 0.9442g, distilled water are fixed
Hold 1000ml volumetric flasks.
(2) levodopa substrate is configured：Take 0.0296g DOPA in 100ml volumetric flasks, with pbs constant volumes.
(3) tyrosine substrate is configured：0.0272g tyrosine and 100ml volumetric flasks are taken, with pbs constant volumes.
(4) tyrosinase is configured：Take 0.3~0.4ml in 25ml volumetric flasks, with pbs constant volumes.
Pay attention to：Tyrosinase is put in always in 0 DEG C of mixture of ice and water, prevents from inactivating, matching while using.
Preliminary checkout：Enzyme activity 1mlPBS+1ml DOPA+1ml tyrosinase+1ml water is tested, is surveyed in absorbance 475nm
It is fixed, it is read per 30s, total 5min obtains trace.Enzyme activity is qualification in 60u/ml~70u/ml, if less than desired enzyme activity
Power is needed in addition tyrosinase, until reaching enzyme activity.
Positive control is kojic acid, configures 500ug/ml.
Table 3
(5) it is averaged according to two groups of data of condition parallel determination of 3 each group of table, inhibiting rate calculation formula is：Inhibit
Rate %=1- { (B1-B2)/(A1-A2) }
Experimental result is as shown in table 4：
Table 4
2.3 human body whitening effects are tested
Method：30 people of Subject Population (using the previous moon from unused any whitening product, skin color is close), age
Between 20 years old to 60 years old, it is divided into 3 groups, first group (embodiment 1, comparative example 1 and comparative example 1 '), second group of (implementation
Example 2, comparative example 2 and comparative example 2 '), third group (embodiment 3, comparative example 3 and comparative example 3 '), every
On the inside of the left arm (or right arm) of lineup fixed area smears respectively be added to 5wt% by each group of each embodiment preparation
The lotion of plant extraction liquid keeps original rule of life during experiment, record is using 4d and uses the data after 42d.
(1) skin shine measures：Skin complexion L values are measured using the skinanalysis apparatus that German CK companies produce, are passed through
L values are measured to assess influence of the product to glossiness.Test result is as shown in table 5.
Table 5
(2) measurement of dermal melanin：The dermal melanin measuring instrument and haematochrome produced using German CK companies is measured
Instrument measures the melanin value in skin, and target area dermal melanin is measured 5 times and is averaged, before and after measurement product use
Melanin numerical value change assesses the white-skinned face function of product.Test result is as shown in table 6.
Table 6
In conclusion the present invention is by tealeaves, Radix Astragali, apple, cucumber and white jade orchid by carrying out mixing extraction, acquisition
Extracting solution is of light color, thermostabilization is good, active constituent is more, has good white-skinned face function.
The above description of the embodiments is intended to facilitate ordinary skill in the art to understand and use the invention.
Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein general
Principle is applied in other embodiment without having to go through creative labor.Therefore, the present invention is not limited to the above embodiments, ability
Field technique personnel announcement according to the present invention, improvement and modification made without departing from the scope of the present invention all should be the present invention's
Within protection domain.
Claims (10)
1. a kind of plant composition for whitening, which is characterized in that the composition includes each of following weight percentage
Component：
2. a kind of plant extraction liquid for whitening, which is characterized in that the method system that the extracting solution can be by including the following steps
It is standby and obtain：Using the plant composition for being used for whitening as described in claim 1 as raw material, extracts and obtain.
3. a kind of preparation method of the plant extraction liquid as claimed in claim 2 for whitening, which is characterized in that the preparation
Method includes the following steps：
Extraction step：The tealeaves weighed, scutellariae,radix, apple, cucumber and white jade orchid composition mixed raw material are impregnated into 2h, using water
Stirring extraction 4h, obtains crude extract at 45 DEG C of formulation；
Microfiltration step：By crude extract successively through 1 μm of ceramic membrane and 0.2 μm of ceramic membrane filter, coarse filtration liquid is obtained；
Ultrafiltration step：By coarse filtration liquid through the film ultrafiltration that molecular cut off is 1500D, filtrate is obtained；
Concentration step：By filtrate through the film nanofiltration that molecular cut off is 150D, concentrate is obtained；
Preparation steps：Water and 1 is added in concentrate, 3 butanediols mix up to the plant extraction liquid.
4. the preparation method for the plant extraction liquid of whitening as claimed in claim 3, which is characterized in that in extraction step,
In the water extraction, the weight ratio of mixed raw material and aqueous solution is 1:8.
5. the preparation method for the plant extraction liquid of whitening as claimed in claim 4, which is characterized in that the aqueous solution is
Aqueous solution including 1wt% cellulases and 1wt% pectases.
6. the preparation method for the plant extraction liquid of whitening as claimed in claim 3, which is characterized in that in microfiltration step,
The operating pressure of the filtering is 10~15bar, temperature≤45 DEG C；In ultrafiltration step, the operating pressure of the filtering is 3~
6bar, temperature≤45 DEG C.
7. the preparation method for the plant extraction liquid of whitening as claimed in claim 3, which is characterized in that in ultrafiltration step,
In the ultra-filtration process, when phegma remains 30%, the water for supplementing equivalent continues ultrafiltration to the amount of diffusate equal to starting extracting solution
Amount.
8. the preparation method for the plant extraction liquid of whitening as claimed in claim 3, which is characterized in that in concentration step,
The operating pressure of the nanofiltration is 10~15bar, temperature≤45 DEG C；The weight ratio of extract and aqueous solution is in the concentrate
1:1.6。
9. the preparation method for the plant extraction liquid of whitening as claimed in claim 3, which is characterized in that the plant obtained
The weight ratio of extract and 1,3 butanediol aqueous solutions is 1 in object extracting solution:16；1,3 butanediol mass concentration in plant extraction liquid
It is 20%.
10. a kind of application as claimed in claim 2 for the plant extraction liquid of whitening in cosmetics.
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| CN111759779A (en) * | 2020-06-10 | 2020-10-13 | 完美（广东）日用品有限公司 | Preparation method and application of aloe flower extract |
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| CN102871898A (en) * | 2012-09-05 | 2013-01-16 | 福建泉州恒泉化妆品有限公司 | Yellowness-improving and lightening and whitening lotion product and preparation method |
| CN103417449A (en) * | 2013-07-15 | 2013-12-04 | 杨正福 | Cucumber-green tea toner |
| CN104042548A (en) * | 2014-06-26 | 2014-09-17 | 广东省保化检测中心有限公司 | Snail grume extract as well as preparation method and application thereof in cosmetics |
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| CN102871898A (en) * | 2012-09-05 | 2013-01-16 | 福建泉州恒泉化妆品有限公司 | Yellowness-improving and lightening and whitening lotion product and preparation method |
| CN103417449A (en) * | 2013-07-15 | 2013-12-04 | 杨正福 | Cucumber-green tea toner |
| CN104042548A (en) * | 2014-06-26 | 2014-09-17 | 广东省保化检测中心有限公司 | Snail grume extract as well as preparation method and application thereof in cosmetics |
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