CN105816396A - Plant composition for skin whitening, plant extracting solution and preparation method thereof - Google Patents
Plant composition for skin whitening, plant extracting solution and preparation method thereof Download PDFInfo
- Publication number
- CN105816396A CN105816396A CN201610371046.3A CN201610371046A CN105816396A CN 105816396 A CN105816396 A CN 105816396A CN 201610371046 A CN201610371046 A CN 201610371046A CN 105816396 A CN105816396 A CN 105816396A
- Authority
- CN
- China
- Prior art keywords
- whitening
- preparation
- extraction liquid
- plant extraction
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a plant composition for skin whitening, a plant extracting solution and a preparation method thereof .The plant composition is prepared from, by weight, 30%-60% of tea leaves, 15%-20% of radix scutellariae roots, 5%-20% of apples, 5%-10% of cucumbers and 5%-20% of magnolia flowers .The plant extracting solution is prepared by extracting the plant composition for skin whitening .The preparation method of the plant extracting solution comprises the steps of extraction, microfiltration, ultrafiltration, concentration and preparation .The plant extracting solution prepared through the method is good in stability, more active ingredients are obtained, and an excellent skin whitening effect of the plant extracting solution applied to cosmetics is achieved.
Description
Technical field
The present invention relates to cosmetic field, relate to a kind of vegetable composition for whitening, plant extraction liquid and preparation method thereof.
Background technology
Along with environmental disruption, the impact that the too much rhythm of life of pollutants in air is irregular and depletion of the ozone layer ultraviolet irradiates, protection skin and whitening are that women never stopped demand, and the object that natural harmless cosmetics people especially pursue.
Tea polyphenols energy shielding of ultraviolet in Folium Camelliae sinensis, remove the free radical that ultraviolet produces, lipoxygenase and lipid peroxidation in suppression skin line plastochondria, thus there is anti-aging effects, experiment shows that tea polyphenols also has antiallergic action and anti-skin allergy effect.
Having inhibitory action to dermatophytes containing the flavone compound such as baicalin, baicalin in Radix Scutellariae, non-oxidizability is strong, and experiment proof has the effect of antiinflammatory and antiallergic action, and, adjuvant-induced arthritis swollen to rat foot and passive cutaneous anaphylaxis, PCA all have inhibitory action.
Fructus Mali pumilae is one of modal fruit, big containing nutrition solubility, is easily absorbed by the body, and wherein malic acid gentleness removes old useless unnecessary cutin, strengthens skin metabolism；Glycine collagen protein and the constituent of elastin laminin；Cysteine is tyrosinase inhibitor, has whitening function；Aspartic acid is the essential amino acids required for cell metabolism；The antioxidant such as Quercetin, citric acid, carotene, the free radical in scavenger cell；Phloretin and phlorhizin in Pericarpium Mali pumilae are tyrosinase inhibitors；Also having the elements such as copper, iodine, manganese, zinc, potassium in Fructus Mali pumilae, human body such as lacks these elements, and skin will be dried, easily splits, very itch.
Containing abundant vitamin C, vitamin E, carotene in Fructus Cucumidis sativi, can resist free radical, vitamin C can reduce the melanin in skin.
Flos micheliae Albae can suppress the skin injury that UVB causes according to the literature, and Flos micheliae Albae flower extract is IC50=13.6% to the inhibitory action of tryrosinase.
The most more whitening extracting solution raw material is mostly single plant material, and action pathway is single, and extracting solution color is deep, and poor heat stability, active component is few.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide a kind of vegetable composition for whitening, plant extraction liquid and preparation method thereof.The present invention is by carrying out mixed extraction by the vegetable composition including Folium Camelliae sinensis, the Radix Astragali, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw., it is thus achieved that plant extraction liquid of light color, thermally-stabilised good, active component is many, has good white-skinned face function.
It is an object of the invention to be achieved through the following technical solutions:
The invention provides a kind of vegetable composition for whitening, described compositions includes each component of following weight percentage:
Described Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. be fresh weight, Folium Camelliae sinensis and Radix Scutellariae be dry weight.
Present invention also offers a kind of plant extraction liquid for whitening, described extracting solution can be prepared by the method comprised the steps: with the above-mentioned vegetable composition for whitening as raw material, extracts and obtains.
Present invention also offers the preparation method of a kind of plant extraction liquid for whitening, described preparation method comprises the following steps:
Extraction step: by the Folium Camelliae sinensis weighed, scutellariae,radix, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. composition mixing raw material soaking 2h, use stirring at water extraction 45 DEG C to extract 4h, obtain crude extract；
Microfiltration step: by crude extract successively through 1 μm ceramic membrane and 0.2 μm ceramic membrane filter, obtain coarse filtration liquid；
Ultrafiltration step: be the membrane ultrafiltration of 1500D through molecular cut off by coarse filtration liquid, obtains filtrate；
Concentration step: by filtrate through the film nanofiltration that molecular cut off is 150D, obtain concentrated solution；
Preparation steps: add water and 1,3 butanediols in concentrated solution, mix and i.e. obtain described plant extraction liquid.
Preferably, in extraction step, in described water extraction, mixing raw material is 1:8 with the weight ratio of aqueous solution.
Preferably, described aqueous solution is to include 1wt% cellulase and the aqueous solution of 1wt% pectase.
Preferably, in microfiltration step, the operation pressure of described filtration is 10～15bar, temperature≤45 DEG C.
Preferably, in ultrafiltration step, the operation pressure of described filtration is 3～6bar, temperature≤45 DEG C.
Preferably, in ultrafiltration step, in described ultra-filtration process, when backflow remains about 30%, the amount of the water continuation ultrafiltration of supplementary equivalent to transudate is equal to initial extraction liquid measure.
Preferably, in concentration step, the operation pressure of described nanofiltration is 10～15bar, temperature≤45 DEG C；In described concentrated solution, extract is 1:1.6 with the weight ratio of aqueous solution.Described extract is the material extracted from mixing raw material.
Preferably, extract and 1 in described prepared plant extraction liquid, the weight ratio of 3 butanediol aqueous solutions is 1:16；In plant extraction liquid, 1,3 butanediol mass concentrations are 20%.Described 1,3 butanediol aqueous solutions are the mixed solution that 1,3 butanediols are formed with water.The step of described preparation is i.e. by adding 1,3 butanediols and water by concentrated solution dilution 10 times.
Present invention also offers the application in cosmetics of a kind of described plant extraction liquid for whitening.
Preferably, described cosmetics include water, emulsion and cream.
Preferably, described plant extraction liquid addition in emulsion is 5wt%.
The dominant mechanism of whitening includes scavenging activated oxygen, suppression tryrosinase, reduction melanin intermediates, the proliferation and differentiation of regulation melanocyte, promotes that horny layer comes off, traditional whitening product effective ingredient mechanism of action is more single, and the most high temperature extraction of plant extraction liquid, extraction efficiency is low, active substance destructible, color is deeper or easy variable color, poor stability.And the present invention selects raw material from multiple lightening mechanism, and select the plant having synergistic function, the technique using enzymolysis and extraction-microfiltration-ultrafiltration-membrance concentration-preparation, improves the yield of active substance, increases the stability of product, practicality, and the technique feature of environmental protection.
Compared with prior art, the present invention has a following beneficial effect:
1, by selecting technique and material combination, activity and the yield of effective ingredient is improved.
2, the reservation (activated carbon decolorizing can cause flavones ingredient to lose in a large number) of little molecular chaperones such as Polyphenols, flavonoid is increased.
3, strengthen the stability of extracting solution, reduce extracting solution variable color in long-term placement process.
4, the salt component in extracting solution is removed, it is to avoid extracting solution adds the generation phenomenon such as viscosity reduction, breakdown of emulsion in cosmetic formulations to.
5, preparation engineering does not use ethanol, resin etc., Environmental Safety, cost-effective.
6, the plant extraction liquid of preparation has extraordinary white-skinned face function in cosmetics.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in those skilled in the art and are further appreciated by the present invention, but limit the present invention the most in any form.It should be pointed out that, to those skilled in the art, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement.These broadly fall into protection scope of the present invention.
Embodiment 1
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 40%, scutellariae,radix 20%, Fructus Mali pumilae 10%, Fructus Cucumidis sativi 10%, white jade Cymbidium ensifolium (L.) Sw. 20%.Described Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. be fresh weight, Folium Camelliae sinensis and Radix Scutellariae be dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
Folium Camelliae sinensis, Radix Scutellariae, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. are soaked 2h, and 8 times of water add 1% cellulase and 1% pectase 45 DEG C stirring extraction 4h；Microfiltration is with 1 μm ceramic membrane filter, then with 0.2 μm ceramic membrane filter, pressure 10bar；Ultrafiltration molecular cut off is the film of 1500D, operates pressure 5bar, and when ultrafiltration to backflow remains about 30%, the amount of the water continuation ultrafiltration of supplementary equivalent to transudate is equal to initial extraction liquid measure；Concentration molecular cut off is the film of 150D, operates pressure 10bar, and temperature keeps≤45 DEG C, nanofiltration to concentrated solution is equivalent to 0.5g/ml (i.e. in concentrated solution, the weight ratio of extract and aqueous solution is 1:1.6)；It is eventually adding 1, concentrated solution is diluted 10 times by 3 butanediols and water, it is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1, the weight ratio of 3 butanediol aqueous solutions is 1:16), 1,3 butanediol mass concentrations are 20%, are the extracting solution (ceramic membrane filter, the temperature of ultrafiltration all keep≤45 DEG C) of this programme.
Embodiment 2
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 60%, scutellariae,radix 15%, Fructus Mali pumilae 10%, Fructus Cucumidis sativi 5%, white jade Cymbidium ensifolium (L.) Sw. 10%.Described Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. be fresh weight, Folium Camelliae sinensis and Radix Scutellariae be dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
Folium Camelliae sinensis, Radix Scutellariae, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. are soaked 2h, and 8 times of water add 1% cellulase and 1% pectase 45 DEG C stirring extraction 4h；Microfiltration is with 1 μm ceramic membrane filter, then with 0.2 μm ceramic membrane filter, pressure 12bar；Ultrafiltration molecular cut off is the film of 1500D, operates pressure 3bar, and when ultrafiltration to backflow remains about 30%, the amount of the water continuation ultrafiltration of supplementary equivalent to transudate is equal to initial extraction liquid measure；Concentration molecular cut off is the film of 150D, operates pressure 12bar, and temperature keeps≤45 DEG C, nanofiltration to concentrated solution is equivalent to 0.5g/ml (i.e. in concentrated solution, the weight ratio of extract and aqueous solution is 1:1.6)；It is eventually adding 1, concentrated solution is diluted 10 times by 3 butanediols and water, it is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1, the weight ratio of 3 butanediol aqueous solutions is 1:16), 1,3 butanediol mass concentrations are 20%, are the extracting solution (ceramic membrane filter, the temperature of ultrafiltration all keep≤45 DEG C) of this programme.
Embodiment 3
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 50%, scutellariae,radix 20%, Fructus Mali pumilae 10%, Fructus Cucumidis sativi 5%, white jade Cymbidium ensifolium (L.) Sw. 15%.Described Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. be fresh weight, Folium Camelliae sinensis and Radix Scutellariae be dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
Folium Camelliae sinensis, Radix Scutellariae, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. are soaked 2h, and 8 times of water add 1% cellulase and 1% pectase 45 DEG C stirring extraction 4h；Microfiltration is with 1 μm ceramic membrane filter, then with 0.2 μm ceramic membrane filter, pressure 15bar；Ultrafiltration molecular cut off is the film of 1500D, operates pressure 6bar, and when ultrafiltration to backflow remains about 30%, the amount of the water continuation ultrafiltration of supplementary equivalent to transudate is equal to initial extraction liquid measure；Concentration molecular cut off is the film of 150D, operates pressure 15bar, and temperature keeps≤45 DEG C, and nanofiltration to concentrated solution is equivalent to 0.5g/ml (i.e. in concentrated solution, the weight ratio of extract and aqueous solution is 1:1.6)；It is eventually adding 1, concentrated solution is diluted 10 times by 3 butanediols and water, it is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1, the weight ratio of 3 butanediol aqueous solutions is 1:16), 1,3 butanediol mass concentrations are 20%, are the extracting solution (ceramic membrane filter, the temperature of ultrafiltration all keep≤45 DEG C) of this programme.
Comparative example 1
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 40%, scutellariae,radix 20%, Fructus Mali pumilae 10%, Fructus Cucumidis sativi 10%, white jade Cymbidium ensifolium (L.) Sw. 20%.Described Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. be fresh weight, Folium Camelliae sinensis and Radix Scutellariae be dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
Folium Camelliae sinensis, Radix Scutellariae, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. are soaked 2h, 60 DEG C of extraction 4h of 8 times of water；Extracting solution is concentrated into proportion 1.4, adds 95 ° of ethanol to 80% precipitate with ethanol, cold preservation 24h；Filtering, filtrate is concentrated into proportion 1.3, adds ethanol to 90 ° of precipitate with ethanol, cold preservation 24h；Filtering, filtrate is concentrated into proportion 1.2, cold preservation 12h；Filtering, filtrate adds water to crude drug 0.5g/ml, cold preservation 24h；Filtering, filtrate adds 80 DEG C of 0.5h of 200 mesh activated carbon, filters, adds 1, and 3 butanediols and water are made into and are equivalent to crude drug 0.05g/ml, and 1,3 butanediol concentration is 20%, to obtain final product.
Comparative example 1 '
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 40%, scutellariae,radix 20%, Fructus Mali pumilae 10%, Fructus Cucumidis sativi 10%, white jade Cymbidium ensifolium (L.) Sw. 20%.Described Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. be fresh weight, Folium Camelliae sinensis and Radix Scutellariae be dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
Folium Camelliae sinensis, Radix Scutellariae, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. are soaked 2h, 8 times of water boil 4h；Add the flocculate with chitosan of 0.3 ‰, be slowly stirred 2h, filter, aging 7 days of filtrate, filter, be concentrated into and be equivalent to 0.5g/ml, add 1,3 butanediols and water, it is made into and is equivalent to crude drug 0.05g/ml, 1,3 butanediol concentration is 20%, to obtain final product.
Comparative example 2
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 40%, scutellariae,radix 20%, Fructus Mali pumilae 40%.Described Fructus Mali pumilae be fresh weight, Folium Camelliae sinensis and Radix Scutellariae be dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
Folium Camelliae sinensis, Radix Scutellariae, Fructus Mali pumilae are soaked 2h, and 8 times of water add 1% cellulase and 1% pectase 45 DEG C stirring extraction 4h；Microfiltration is with 1 μm ceramic membrane filter, then with 0.2 μm ceramic membrane filter, pressure 12bar；Ultrafiltration molecular cut off is the film of 1500D, operates pressure 3bar, and when ultrafiltration to backflow remains about 30%, the amount of the water continuation ultrafiltration of supplementary equivalent to transudate is equal to initial extraction liquid measure；Concentration molecular cut off is the film of 150D, operates pressure 12bar, and temperature keeps≤45 DEG C, nanofiltration to concentrated solution is equivalent to 0.5g/ml (i.e. in concentrated solution, the weight ratio of extract and aqueous solution is 1:1.6)；It is eventually adding 1, concentrated solution is diluted 10 times by 3 butanediols and water, it is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1, the weight ratio of 3 butanediol aqueous solutions is 1:16), 1,3 butanediol mass concentrations are 20%, are the extracting solution (ceramic membrane filter, the temperature of ultrafiltration all keep≤45 DEG C) of this programme.
Comparative example 2 '
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 40%, scutellariae,radix 20%, Fructus Mali pumilae 40%.Described Fructus Mali pumilae be fresh weight, Folium Camelliae sinensis and Radix Scutellariae be dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
Folium Camelliae sinensis, Radix Scutellariae, Fructus Mali pumilae are soaked 2h, 8 times of water boil 4h；Add the flocculate with chitosan of 0.3 ‰, be slowly stirred 2h, filter, aging 7 days of filtrate, filter, be concentrated into and be equivalent to 0.5g/ml, add 1,3 butanediols and water, it is made into and is equivalent to crude drug 0.05g/ml, 1,3 butanediol concentration is 20%, to obtain final product.
Comparative example 3
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 100%.Described Folium Camelliae sinensis is dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
By infusion of tea 2h, 8 times of water add 1% cellulase and 1% pectase 45 DEG C stirring extraction 4h；Microfiltration is with 1 μm ceramic membrane filter, then with 0.2 μm ceramic membrane filter, pressure 12bar；Ultrafiltration molecular cut off is the film of 1500D, operates pressure 3bar, and when ultrafiltration to backflow remains about 30%, the amount of the water continuation ultrafiltration of supplementary equivalent to transudate is equal to initial extraction liquid measure；Concentration molecular cut off is the film of 150D, operates pressure 12bar, and temperature keeps≤45 DEG C, nanofiltration to concentrated solution is equivalent to 0.5g/ml (i.e. in concentrated solution, the weight ratio of extract and aqueous solution is 1:1.6)；It is eventually adding 1, concentrated solution is diluted 10 times by 3 butanediols and water, it is made into and is equivalent to crude drug 0.05g/ml (i.e. extract and 1, the weight ratio of 3 butanediol aqueous solutions is 1:16), 1,3 butanediol mass concentrations are 20%, are the extracting solution (ceramic membrane filter, the temperature of ultrafiltration all keep≤45 DEG C) of this programme.
Comparative example 3 '
The present embodiment relates to a kind of vegetable composition with whitening function, and described compositions is by each component of following weight percent content: Folium Camelliae sinensis 100%.Described Folium Camelliae sinensis is dry weight.
The present embodiment further relates to a kind of plant extraction liquid for whitening, above-mentioned vegetable composition extract as raw material and obtain.
The preparation method of described plant extraction liquid is as follows:
The present embodiment relates to a kind of plant extract liquid mixture with whitening function, including each component of following weight percent content: Folium Camelliae sinensis 100%.
By infusion of tea 2h, 8 times of water boil 4h；Add the flocculate with chitosan of 0.3 ‰, be slowly stirred 2h, filter, aging 7 days of filtrate, filter, be concentrated into and be equivalent to 0.5g/ml, add 1,3 butanediols and water, it is made into and is equivalent to crude drug 0.05g/ml, 1,3 butanediol concentration is 20%, to obtain final product.
Compliance test result is tested
1, the embodiment of the present invention is as shown in table 1 with the stability experiment result of comparative example.
Table 1
2, the Efficacy experiments of the present invention
2.1 antioxidation in vitro experiment DPPH free radical scavenging experiments
Principle: DPPH method was suggested in 1958, is widely used in quantitative determination Biosample and the oxidation resistance of food.This method is to have single electron according to DPPH free radical, has the last one to absorb at 517nm, and its alcoholic solution is the characteristic of purple.In the presence of having free radical scavenger, making it absorb fade away owing to matching with its single electron, the electron amount that its fading extent accepts with it becomes quantitative relationship, thus available spectrophotometer carries out quick quantitative analysis.
Experiment flow:
(1) configuration DPPH solution: accurately weigh 20mgDPPH, with dehydrated alcohol constant volume in 250ml volumetric flask, obtain the DPPH solution that concentration is 2.0 × 10-4mol/L, keep in Dark Place standby.
(2) 2ml sample liquid is taken respectively in tool plug test tube, add the DPPH solution that 2ml is configured, mix homogeneously, in 25 DEG C of water-baths after airtight standing 30min, pour the cuvette that optical path is 1cm into, go out to record its absorbance A i at 517nm, survey 2ml sample simultaneously and add absorbance A j of 2ml ethanol, and 2mlDPPH solution adds the absorbance A 0 of 2ml redistilled water.Often survey a used in amounts and to survey three panel datas.Sample liquid uses the extracting solution in embodiment and comparative example.
(3) clearance rate of DPPH=[1 one (Ai-Aj)/A0] * 100%
Experimental result is as shown in table 2:
Table 2
2.2 Tyrosinase inhibition assays
(1) configuration phosphate buffer (pbs) (Ph=6.8) takes KH2PO46.80g, NaOH0.9442g, distilled water constant volume 1000ml volumetric flask.
(2) configuration levodopa substrate: take 0.0296g DOPA in 100ml volumetric flask, use pbs constant volume.
(3) configuration tyrosine substrate: take 0.0272g tyrosine and 100ml volumetric flask, use pbs constant volume.
(4) configuration tryrosinase: take 0.3～0.4ml in 25ml volumetric flask, use pbs constant volume.
Note: tryrosinase is put in all the time in 0 DEG C of mixture of ice and water, prevent inactivation, matching while using.
Preliminary checkout: tested enzyme vigor 1mlPBS+1ml DOPA+1ml tryrosinase+1ml water, measures at absorbance 475nm, every 30s reading, and 5min, draws trace altogether.Enzyme activity is qualified at 60u/ml～70u/ml, if less than the enzyme activity required, needs adding tryrosinase, until reaching enzyme activity.
Positive control is kojic acid, configures 500ug/ml.
Table 3
(5) the two groups of data of condition parallel assay respectively organized according to table 3 are averaged, and suppression ratio computing formula is: suppression ratio %=1-{ (B1-B2)/(A1-A2) }
Experimental result is as shown in table 4:
Table 4
2.3 physical beauty's white effect tests
Method: Subject Population 30 people (uses the previous moon never to use any whitening product, skin color is close), between 20 years old to 60 years old age, it is divided into 3 groups, first group of (embodiment 1, comparative example 1 and comparative example 1 '), second group of (embodiment 2, comparative example 2 and comparative example 2 '), 3rd group of (embodiment 3, comparative example 3 and comparative example 3 '), left arm (or right arm) inner side fixed area every lineup smears the emulsion of the plant extraction liquid prepared by each group of each embodiment that with the addition of 5wt% respectively, original rule of life is kept during experiment, record uses the data after 4d and use 42d.
(1) skin shine measures: the skinanalysis apparatus using CK company of Germany to produce measures skin complexion L-value, assesses the product impact on glossiness by measuring L-value.Test result is as shown in table 5.
Table 5
(2) mensuration of dermal melanin: use the dermal melanin measuring instrument of CK company of Germany production and red pigment measuring instrument to measure the melanin value in skin, target site dermal melanin is measured and is averaged for 5 times, and the melanin change in value before and after being used by measurement product assesses the white-skinned face function of product.Test result is as shown in table 6.
Table 6
In sum, the present invention is by carrying out mixed extraction by Folium Camelliae sinensis, the Radix Astragali, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw., it is thus achieved that extracting solution of light color, thermally-stabilised good, active component is many, has good white-skinned face function.
The above-mentioned description to embodiment is to be understood that for ease of those skilled in the art and use invention.These embodiments obviously easily can be made various amendment by person skilled in the art, and General Principle described herein is applied in other embodiments without through performing creative labour.Therefore, the invention is not restricted to above-described embodiment, those skilled in the art should be within protection scope of the present invention according to the announcement of the present invention, the improvement made without departing from scope and amendment.
Claims (10)
1. the vegetable composition for whitening, it is characterised in that described compositions includes each component of following weight percentage:
2. the plant extraction liquid for whitening, it is characterised in that described extracting solution can be prepared by the method comprised the steps: with the vegetable composition for whitening as claimed in claim 1 as raw material, extract and obtain.
3. the preparation method of the plant extraction liquid being used for whitening as claimed in claim 2, it is characterised in that described preparation method comprises the following steps:
Extraction step: by the Folium Camelliae sinensis weighed, scutellariae,radix, Fructus Mali pumilae, Fructus Cucumidis sativi and white jade Cymbidium ensifolium (L.) Sw. composition mixing raw material soaking 2h, use stirring at water extraction 45 DEG C to extract 4h, obtain crude extract；
Microfiltration step: by crude extract successively through 1 μm ceramic membrane and 0.2 μm ceramic membrane filter, obtain coarse filtration liquid；
Ultrafiltration step: be the membrane ultrafiltration of 1500D through molecular cut off by coarse filtration liquid, obtains filtrate；
Concentration step: by filtrate through the film nanofiltration that molecular cut off is 150D, obtain concentrated solution；
Preparation steps: add water and 1,3 butanediols in concentrated solution, mix and i.e. obtain described plant extraction liquid.
4. the preparation method of the plant extraction liquid for whitening as claimed in claim 3, it is characterised in that in extraction step, in described water extraction, mixing raw material is 1:8 with the weight ratio of aqueous solution.
5. the preparation method of the plant extraction liquid for whitening as claimed in claim 4, it is characterised in that described aqueous solution is to include 1wt% cellulase and the aqueous solution of 1wt% pectase.
6. the preparation method of the plant extraction liquid for whitening as claimed in claim 3, it is characterised in that in microfiltration step, the operation pressure of described filtration is 10～15bar, temperature≤45 DEG C；In ultrafiltration step, the operation pressure of described filtration is 3～6bar, temperature≤45 DEG C.
7. the preparation method of the plant extraction liquid for whitening as claimed in claim 3, it is characterised in that in ultrafiltration step, in described ultra-filtration process, when backflow remains 30%, the amount of the water continuation ultrafiltration of supplementary equivalent to transudate extracts liquid measure equal to initial.
8. the preparation method of the plant extraction liquid for whitening as claimed in claim 3, it is characterised in that in concentration step, the operation pressure of described nanofiltration is 10～15bar, temperature≤45 DEG C；In described concentrated solution, extract is 1:1.6 with the weight ratio of aqueous solution.
9. the preparation method of the plant extraction liquid for whitening as claimed in claim 3, it is characterised in that extract and 1 in described prepared plant extraction liquid, the weight ratio of 3 butanediol aqueous solutions is 1:16；In plant extraction liquid, 1,3 butanediol mass concentrations are 20%.
10. one kind is used for the application in cosmetics of the plant extraction liquid of whitening as claimed in claim 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610371046.3A CN105816396B (en) | 2016-05-30 | 2016-05-30 | For the plant composition of whitening, plant extraction liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610371046.3A CN105816396B (en) | 2016-05-30 | 2016-05-30 | For the plant composition of whitening, plant extraction liquid and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105816396A true CN105816396A (en) | 2016-08-03 |
| CN105816396B CN105816396B (en) | 2018-09-25 |
Family
ID=56531451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610371046.3A Active CN105816396B (en) | 2016-05-30 | 2016-05-30 | For the plant composition of whitening, plant extraction liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105816396B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107412046A (en) * | 2017-06-21 | 2017-12-01 | 上海清轩生物科技有限公司 | The preparation method and its lightening compositions of peach blossom extract solution |
| CN111759779A (en) * | 2020-06-10 | 2020-10-13 | 完美（广东）日用品有限公司 | Preparation method and application of aloe flower extract |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102871898A (en) * | 2012-09-05 | 2013-01-16 | 福建泉州恒泉化妆品有限公司 | Yellowness-improving and lightening and whitening lotion product and preparation method |
| CN103417449A (en) * | 2013-07-15 | 2013-12-04 | 杨正福 | Cucumber-green tea toner |
| CN104042548B (en) * | 2014-06-26 | 2018-06-19 | 广东省保化检测中心有限公司 | A kind of snail mucus extract and preparation method thereof and the application in cosmetics |
-
2016
- 2016-05-30 CN CN201610371046.3A patent/CN105816396B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107412046A (en) * | 2017-06-21 | 2017-12-01 | 上海清轩生物科技有限公司 | The preparation method and its lightening compositions of peach blossom extract solution |
| CN111759779A (en) * | 2020-06-10 | 2020-10-13 | 完美（广东）日用品有限公司 | Preparation method and application of aloe flower extract |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105816396B (en) | 2018-09-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106473987B (en) | The anti-ageing composition of whitening and cosmetics | |
| CN104473828B (en) | A kind of dragon fruit stem end moisture retention liquid and preparation method thereof | |
| CN107496245B (en) | Anti-aging liposome composition and preparation method and application thereof | |
| KR20150139688A (en) | The Extract Of The Citrus grandis Osbeck Having Skin Whitening Activity And Cosmetic Composition Containing The Same | |
| CN106389175A (en) | Nourishing and moisturizing lotion containing rose essential oil | |
| KR101936294B1 (en) | Composition comprising for skin-whitening and anti-wrinkling extract of Rumex acetosella L. or extract of Hydrangea serrata | |
| KR101682394B1 (en) | Cosmetic composition comprising color food extracts | |
| CN110664649A (en) | Whitening composition and application thereof | |
| CN110236981A (en) | A kind of whitening plant polyose composition and its application | |
| CN103784356B (en) | The application in preparing antioxidation cosmetic product of the Fructus Mangifera Indicae active site | |
| CN107440963A (en) | A kind of persimmon leaf flavone with whitening anti-aging activity and its preparation method and application | |
| KR20180130881A (en) | Manufacturing Method of Japanese Rose Flower Extract and Rose Flower Extract for Whitening Cosmetic Preparations containing these Extracts | |
| CN108714126A (en) | Skin care compositions and the preparation method and application thereof with moisturizing and anti-oxidation efficacy | |
| CN105708759B (en) | A kind of pure natural anti-oxidant whitening multiple-effect washing cream containing peony seed oil and preparation method thereof | |
| CN105816396A (en) | Plant composition for skin whitening, plant extracting solution and preparation method thereof | |
| CN107468568A (en) | A kind of persimmon leaf polyphenol with whitening sun protection activity and its preparation method and application | |
| CN108324667A (en) | Composition and anti-aging Skin whitening care cosmetics containing light black huge marine algae extract | |
| CN107802545A (en) | A kind of anti-oxidant and lightening compositions and the skin care item containing said composition | |
| KR20090103041A (en) | Cosmetic Composition Containing Ulmus macrocarpa Hance Extract | |
| KR20120006804A (en) | Cosmetic composition containing broussonetia extract for anti-aging | |
| CN109010572A (en) | A kind of Chinese medicine composition and its preparation method and application with sun-proof synergy and anti-aging effects | |
| KR101070708B1 (en) | Method of extracting for sea silt extract and whitening functional cosmetic composition containing the extract | |
| KR20190075407A (en) | A cosmetic composition comprising a natural component and an oil component | |
| Witaszczyk et al. | Usefulness of aloe vera () as a potential ingredient of cosmetic preparations | |
| JP3156928B1 (en) | Skin tightening agents and cosmetics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 201612 Building 2, 758 Xinge Road, Xinqiao Town, Songjiang District, Shanghai Patentee after: Shanghai linqingxuan Biotechnology Co., Ltd Address before: 201612 Building 2, 758 Xinge Road, Xinqiao Town, Songjiang District, Shanghai Patentee before: SHANGHAI FOREST CABIN BIOLOGICAL-TECH Co.,Ltd. |