CN107334944A - Compound flower extract and preparation method thereof - Google Patents
Compound flower extract and preparation method thereof Download PDFInfo
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- CN107334944A CN107334944A CN201610277799.8A CN201610277799A CN107334944A CN 107334944 A CN107334944 A CN 107334944A CN 201610277799 A CN201610277799 A CN 201610277799A CN 107334944 A CN107334944 A CN 107334944A
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Classifications
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- Birds (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a kind of compound flower extract, is obtained by peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus through solvent extraction.Compound flower extract provided by the invention is obtained by peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus through solvent extraction, through the inventors discovered that, the extract that above-mentioned compound flower obtains through solvent extraction has good collaboration tyrosinase inhibitory action, while also has good radicals scavenging effect and anti-aging effects；And this extract component is natural, have no side effect, have a good application prospect.
Description
Technical field
The present invention relates to technical field of plant extraction, more particularly, to compound flower extract and preparation method thereof.
Background technology
With the raising of people's living standard and aesthetical standard, increasing people pursues, thirsted for the flesh of pale and clean profit
Skin.Meanwhile consumer is not only only that its is both effectiveness to the focal point of cosmetics, while also more focus on its security.Plant
The beauty method of cosmetics and back to nature is favored by more and more consumers.
Skin is due in the external environments such as ultraviolet, in active oxygen caused by ultraviolet and other external environments
Active oxygen, can all produce various free radicals in skin, cause to skin oxidative stress damage, dermatitis can be induced in short term
The skin disorders such as disease, the aging of skin will be caused for a long time.Antioxidant is with the material for catching free radical ability, can remove skin
Living radical in skin, mitigate skin oxidative stress damage caused by free radical, so as to improve as caused by oxidative stress etc.
Skin aging.The oxidation-resistant active ingredient of natural origin, the advantages that due to security, wide material sources, by domestic and international researcher
Attention.
The color of skin depends primarily on the summation of skin pigment, and main component is melanin in skin pigment.Human body can
To generate two kinds of melanin：Eumelanin and false melanin.Eumelanin is the perceived color for causing skin, hair and eyes
The main reason for.In general, eumelanin is by gene regulation, is additionally influenceed by the UV stimulations exposed, with
The growth at age, pigmented degree are consequently increased.Pigmented process is a kind of complicated process, wherein key exists
In the synthesis of melanin, the control of the synthesis of melanin by tyrosinase (EC 1.14.18.1).By suppressing tyrosinase
Activity, slow down the generation of melanin, be reduce pigmentation most directly and maximally effective method.
Corium aging, which shows as corium, removes power to xenobiotics and declines, and dermis thickness is thinning, collagen and elasticity
Albumen synthesis reduces, decomposes increase, catabolic enzyme increased activity.These phenomenons are all reduced and secreted with fibroblast number and synthesize
Function reduction is abnormal relevant.Human skin fibroblasts are most important cells in dermis of skin reticular layer, are skin senescences
One of with the main repair cell after cell damage.It is not only able to promote migration, propagation and the differentiation of epidermal cell, moreover it is possible to point
Substantial amounts of collagen, elastomer albumen and various kinds of cell reparative factor are secreted, there is powerful self-renewal capacity, so as to repair
The skin of multiple aging.
In the abundant Flower Resources of China, there are numerous species that there is diversified medical value, can prevent and cure diseases.
Some are recorded in medical ancient books and records by successive dynasties medicine scholar, and some have been listed in conventional Chinese herbal medicine；It is but also also quite a lot of
Flowers be used as medicine and be not but disclosed and record.
Prior art ZL200680055582.7 discloses the anti-oxidant combination for the material for extracting from Acacia bark
Thing, but it does not disclose that whether it has tyrosinase inhibitory action.Therefore, exploitation has good tyrosinase inhibitory action
Flower extract or compound flower extract be necessary.
The content of the invention
In view of this, the technical problem to be solved in the present invention is to provide compound flower extract, provided by the invention compound
Flower extract has good tyrosinase inhibitory action.
The invention provides a kind of compound flower extract, by peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach
Flower, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus obtain through solvent extraction.
Preferably, the peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, golden hairpin stone
The mass ratio of dry measure used in former times orchid and sweet osmanthus is（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1~5）：（0.1~
1）:（1~5）.
Preferably, the solvent is ethanol or water.
The invention provides a kind of preparation method of compound flower extract, including：
By peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus
Mixing, obtains mixture, mixture is obtained into compound flower extract through solvent extraction.
Preferably, the solvent is ethanol, and the ethanol is the ethanol water that volumn concentration is 10% ~ 80%.
Preferably, the quality g of the mixture and the volume mL of ethanol ratio are 15 ~ 25:150~250.
The invention provides the compound flower extract described in above-mentioned technical proposal or the compound flower described in above-mentioned technical proposal
Application of the compound flower extract that the preparation method of extract is prepared in free radical scavenger is prepared.
The invention provides the compound flower extract described in above-mentioned technical proposal or the compound flower described in above-mentioned technical proposal
Application of the compound flower extract that the preparation method of extract is prepared in tyrosinase inhibitor is prepared.
The invention provides the compound flower extract described in above-mentioned technical proposal or the compound flower described in above-mentioned technical proposal
Application of the compound flower extract that the preparation method of extract is prepared in fibrocyte proliferation accelerator is prepared.
Present invention also offers a kind of food/pharmaceutical/cosmetics of whitening, including the compound flower described in above-mentioned technical proposal
The compound flower extract that the preparation method of compound flower extract described in extract or above-mentioned technical proposal is prepared.
Compared with prior art, the invention provides a kind of compound flower extract, by peony, lotus flower, rose, Bai Shan
Camellia, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus extract to obtain through organic solvent.The present invention provides
Compound flower extract by peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII
Orchid and sweet osmanthus extract to obtain through organic solvent, through the inventors discovered that, above-mentioned compound flower carries through what organic solvent extracted to obtain
Take thing that there is good collaboration tyrosinase inhibitory action, while also there is good radicals scavenging effect and anti-aging to make
With；And this extract component is natural, have no side effect, have a good application prospect.
Embodiment
The invention provides a kind of compound flower extract, by peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach
Flower, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus obtain through solvent extraction.
Compound flower extract provided by the invention, by peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum
Flower, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus obtain through solvent extraction, and the solvent is preferably ethanol or water, and the ethanol is excellent
Elect the ethanol water that volumn concentration is 10% ~ 80%, more preferably 20% ~ 75% ethanol water as.
The present invention is for the peony, lotus flower, rose, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid
It is well known to those skilled in the art with the kind of sweet osmanthus and source without limiting.
BAISHACHA flower of the present invention is preferably southwestern BAISHACHA flower.
In the present invention, it is preferred to, the peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, people
Ginseng flower, HERBA DENDROBII orchid and the mass ratio of sweet osmanthus are（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1
~5）：（0.1~1）:（1~5）, it is furthermore preferred that the peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom,
The mass ratio of HERBA DENDROBII orchid and sweet osmanthus is（3~5）:（3~5）:（1~2）:（1~2）:（1~2）:（1~2）:（1~2）:（3~5）：
（0.1~0.5）:（3~5）.
The invention provides a kind of preparation method of compound flower extract, including：
By peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus
Mixing, obtains mixture, and mixture is extracted through organic solvent to obtain compound flower extract.
The preparation method of compound flower extract provided by the invention first by peony, lotus flower, rose, BAISHACHA flower,
Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus mixing, obtain mixture.
The present invention is for the peony, lotus flower, rose, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid
It is well known to those skilled in the art with the kind of sweet osmanthus and source without limiting.
BAISHACHA flower of the present invention is preferably southwestern BAISHACHA flower.
In the present invention, it is preferred to, the peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, people
Ginseng flower, HERBA DENDROBII orchid and the mass ratio of sweet osmanthus are（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1~5）:（1
~5）：（0.1~1）:（1~5）, it is furthermore preferred that the peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom,
The mass ratio of HERBA DENDROBII orchid and sweet osmanthus is（3~5）:（3~5）:（1~2）:（1~2）:（1~2）:（1~2）:（1~2）:（3~5）：
（0.1~0.5）:（3~5）.
In the present invention, mixture is obtained into compound flower extract through solvent extraction.
The solvent is preferably ethanol or water, and the ethanol is preferably that the ethanol that volumn concentration is 10% ~ 80% is water-soluble
Liquid, more preferably 20% ~ 75% ethanol water.
When solvent is ethanol, mixture is extracted through ethanol, concentrated, and obtains alcohol extract.
In the present invention, the quality g of the mixture and the volume mL of ethanol ratio are preferably 15 ~ 25:150~250；
More preferably 17 ~ 23:160~240.
In the present invention, the extraction is preferably ultrasound assisted extraction, and the ultrasonic power is preferably 400 ~ 500w, more
Preferably 450 ~ 500w.
In the present invention, the extraction time is preferably 1 ~ 3 time, more preferably 2 ~ 3 times.
In the present invention, the temperature of the ultrasonic extraction is preferably 20 ~ 40 DEG C, more preferably 25 ~ 35 DEG C.The ultrasound carries
The time taken is preferably every time 20 ~ 40min, more preferably 25 ~ 35min.
The present invention after extraction, preferably merging filtrate, concentration.
Concentration of the present invention is preferably rotary evaporation concentration, and the present invention does not enter for the device of rotary evaporation concentration
Row limits, well known to those skilled in the art.
The temperature of the rotary evaporation concentration is preferably 60 DEG C ~ 75 DEG C；The time is preferably 60min ~ 90min.
Compound flower extract is obtained after concentration.
When solvent is water, mixture is extracted through water, concentrated, and alcohol precipitation, obtains water extract.
In the present invention, the volume mL of the quality g of the mixture and water ratio is preferably 15 ~ 25:150~250；It is more excellent
Elect 17 ~ 23 as:160~240.
The Extracting temperature is preferably 40 ~ 90 DEG C, more preferably 50 ~ 80 DEG C；The extraction time is preferably 1 ~ 3 time, more
Preferably 2 times.The extraction time is preferably the min of 60min ~ 150.
Filtered after extraction, concentrate, obtain concentrate.
Concentrate is mixed with ethanol, precipitated, filtrate reclaims to obtain water extract.
The quality g of the concentrate and the volume mL of ethanol ratio are 1:3~4.
The concentration of the ethanol is preferably 85% ~ 95%, and more preferably 90% ~ 95%.
Concentrate is stood after being mixed with ethanol, filtering, and filtrate recycling ethanol freezes to without alcohol taste, 0.45 μm of membrane filtration, filtrate
It is dry to obtain water extract.
Concentration of the present invention is preferably rotary evaporation concentration, and the present invention does not enter for the device of rotary evaporation concentration
Row limits, well known to those skilled in the art.
The invention provides a kind of compound flower extract, by peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach
Flower, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus extract to obtain through organic solvent.Compound flower extract provided by the invention
By peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus through having
Solvent is extracted to obtain, through the inventors discovered that, above-mentioned compound flower through organic solvent extract obtained extract have it is good
Tyrosinase inhibitory action is cooperateed with, while also there are good radicals scavenging effect and anti-aging effects；And this extract
Composition is natural, has no side effect, and has a good application prospect.
The invention provides the compound flower extract described in above-mentioned technical proposal or the compound flower described in above-mentioned technical proposal
Application of the compound flower extract that the preparation method of extract is prepared in free radical scavenger is prepared.
The invention provides the compound flower extract described in above-mentioned technical proposal or the compound flower described in above-mentioned technical proposal
Application of the compound flower extract that the preparation method of extract is prepared in tyrosinase inhibitor is prepared.
The invention provides the compound flower extract described in above-mentioned technical proposal or the compound flower described in above-mentioned technical proposal
Application of the compound flower extract that the preparation method of extract is prepared in fibrocyte proliferation accelerator is prepared.
Present invention also offers a kind of food/pharmaceutical/cosmetics of whitening, including the compound flower described in above-mentioned technical proposal
The compound flower extract that the preparation method of compound flower extract described in extract or above-mentioned technical proposal is prepared.
The radicals scavenging effect for the compound flower extract for determining to obtain present invention preferably employs in the following manner, tyrosinase
Inhibitory action and anti-aging effects：
Radicals scavenging acts on：
1- diphenyl -2- trinitrophenyl-hydrazines（DPPH）It is a kind of nitrogen center organic free radical of stabilization.DPPH methods were in quilt in 1958
It is proposed, measure the anti-aging ability for determining Biosample, classification material and food with being widely used in.This method is according to DPPH free radicals
There is single electron, there is the last one absorption at 517nm, its alcoholic solution is in the characteristic of purple.In the presence of having free radical scavenger, due to
Matched with its single electron and its absorption is faded away, its fading extent and the electron amount that it receives into quantitative relationship, thus
Quick quantitative analysis can be carried out with spectrophotometer, to detect radicals scavenging situation, so as to evaluate the anti-aging energy of sample
Power.
Tyrosinase inhibitory action：
The color of skin comes from the melanin of keratinocyte memory storage.In general, people's colour of skin more than melanin is stored
It is deeper, also more it is protected, away from solar irradiation.Melanin quality and quantity is in the weight that dermal melanin is decision skin color
Want factor.B16 mouse melanin tumor cells, the effect life of DOPA tyrosinase in melanoma cells are cultivated in 96 orifice plates
Into melanin.Active matter is evaluated come the content of in situ detection melanin by the absorbance at spectrophotometry 405nm
Effect.Tyrosinase is baroque cupric oxidoreducing enzyme, is widely present in microorganism, animals and plants and human body.
Tyrosinase is the key enzyme of skin synthesis of melanin in human body.The mistake of generation melanin is reacted in tyrosinase
Cheng Zhong, the catalytic action of tyrosinase occur mainly in that tyrosine is converted into DOPA and DOPA is converted into DOPA quinone the two are anti-
Ying Zhong.B16 mouse melanin tumor cells are cultivated in 96 orifice plates, DOPA is raw in the presence of tyrosinase in melanoma cells
Into DOPA quinone.The DOPA quinone of formation can generate coloring matter, pass through light splitting with 3-methyl-2-benzothiazole hydrazone coupling reaction
Photometry determines absorbance to detect the activity of tyrosinase, so as to evaluate effect of the active matter to tyrosinase.
Anti-aging effects：
The extract of preparation is configured to solution with deionized water, is added in human fibroblasts nutrient solution, with without sample
Deionized water is blank control.Culture 48 hours after, with mtt assay to cell dyeing after, with ELIASA measure 550nm at extinction
Degree, the proliferation rate of blank control is 100%, reference blank control, assesses the proliferation function to human fibroblasts.Take simultaneously thin
Born of the same parents' supernatant sample, the generation of kit measurement collagen being determined with I-type collagen, the production rate of blank control is 100%,
Reference blank compares, and assesses and synthesizes facilitation to the type i collagen of human fibroblasts.
In order to further illustrate the present invention, with reference to embodiments to compound flower extract provided by the invention and its preparation
Method is described in detail.
Embodiment 1
By 10g peonies, 10g lotus flowers, 10g roses, 10g BAISHACHAs flower, 10g Jasmines, 10g peach blossoms, 10g plum blossoms, 10g people
Ginseng flower, 1g HERBA DENDROBIIs orchid and the mixing of 10g sweet osmanthus, obtain mixture, prepare two parts of said mixtures, a mixture is passed through
30% 910 mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 18g
Compound flower alcohol extracting thing；Another mixture is extracted into 120 min through 910 mL water, 50 DEG C of water, filtering, is condensed into 100mL solution
300mL 95% ethanol is added afterwards, is stood overnight, and filtrate filtrate freeze-drying, obtains 16 through 0.45 μm of filtering with microporous membrane
G compound flower water extracts.
Embodiment 2
By 30g peonies, 30g lotus flowers, 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 30g people
Ginseng flower, 5g HERBA DENDROBIIs orchid and the mixing of 30g sweet osmanthus, obtain mixture, prepare two parts of said mixtures, a mixture is passed through
70% 2750 mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 56
G compound flower alcohol extracting things；Another mixture is extracted into 120 min through 70 DEG C of water of 2750mL water, filtering, it is molten to be condensed into 300mL
900mL 95% ethanol is added after liquid, is stood overnight, filtrate filtrate freeze-drying, obtains through 0.45 μm of filtering with microporous membrane
50 g compound flower water extracts.
Embodiment 3
By 50g peonies, 50g lotus flowers, 50g roses, 50g BAISHACHAs flower, 50g Jasmines, 50g peach blossoms, 50g plum blossoms, 50g people
Ginseng flower, 10g HERBA DENDROBIIs orchid and the mixing of 50g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture
Ethanol through 80% 4600 mL, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains
93 g compound flower alcohol extracting things；Another mixture is extracted into 120 min through 70 DEG C of water of 4600mL water, filtering, is condensed into 500mL
1500mL 95% ethanol is added after solution, is stood overnight, filtrate filtrate freeze-drying, obtains through 0.45 μm of filtering with microporous membrane
To 85g compound flower water extracts.
Comparative example 1
By 30g lotus flowers, 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 30g flower of Panax ginseng, 5g gold
Hairpin stem of noble dendrobium orchid and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture through 70%
2450mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 49g compound flowers
Alcohol extracting thing；By another mixture through 70 DEG C of water extraction 120min of 2450mL water, filtering, added after being condensed into 300mL solution
900mL 95% ethanol, stands overnight, and filtrate filtrate freeze-drying, obtains 45g compound flowers through 0.45 μm of filtering with microporous membrane
Water extract.
Comparative example 2
By 30g peonies, 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 30g flower of Panax ginseng, 5g
HERBA DENDROBII orchid and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture through 70%
2450 mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, it is compound to obtain 47 g
Flower alcohol extracting thing；By another mixture through 70 DEG C of water extraction 120min of 2450mL water, filtering, add after being condensed into 300mL solution
Enter to be condensed into add after 300mL solution after concentration to add 900mL 95% ethanol, stand overnight, filtrate is filtered through 0.45 μm of micropore
Membrane filtration, filtrate freeze-drying, obtains 43g compound flower water extracts.
Comparative example 3
By 30g peonies, 30g lotus flowers, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 30g flower of Panax ginseng, 5g gold
Hairpin stem of noble dendrobium orchid and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture through the 2450 of 70%
ML ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 53g compound flower alcohol extractings
Take thing；Another mixture is extracted into 120 min through 70 DEG C of water of 2450mL water, filtering, concentration is added after being condensed into 300mL solution
Added after into 300mL solution after concentrating and add 900mL 95% ethanol, stood overnight, filtrate is through 0.45 μm of miillpore filter mistake
Filter, filtrate freeze-drying, obtains 49 g compound flower water extracts.
Comparative example 4
By 30g peonies, 30g lotus flowers, 30g roses, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 30g flower of Panax ginseng, 5g Jin Chai
Stem of noble dendrobium orchid and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture through 70% 2450mL
Ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 48 g compound flower alcohol extractings
Take thing；By another mixture through 70 DEG C of water extraction 120min of 2450mL water, filtering, concentration is added after being condensed into 300mL solution
900mL 95% ethanol is added afterwards, is stood overnight, and filtrate filtrate freeze-drying, obtains 44g through 0.45 μm of filtering with microporous membrane
Compound flower water extract.
Comparative example 5
By 30g peonies, 30g lotus flowers, 30g roses, 30g BAISHACHAs flower, 30g peach blossoms, 30g plum blossoms, 30g flower of Panax ginseng, 5g gold
Hairpin stem of noble dendrobium orchid and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture through 70%
2450mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 53g compound flowers
Alcohol extracting thing；By another mixture through 70 DEG C of water extraction 120min of 2450mL water, filtering, added after being condensed into 300mL solution
900mL 95% ethanol is added after concentration, is stood overnight, filtrate filtrate freeze-drying, obtains through 0.45 μm of filtering with microporous membrane
To 49g compound flower water extracts.
Comparative example 6
By 30g peonies, 30g lotus flowers, 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g plum blossoms, 30g flower of Panax ginseng, 5g
HERBA DENDROBII orchid and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture through 70%
2450mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 48g compound flowers
Alcohol extracting thing；By another mixture through 70 DEG C of water extraction 120min of 2450mL water, filtering, added after being condensed into 300mL solution
900mL 95% ethanol is added after concentration, is stood overnight, filtrate filtrate freeze-drying, obtains through 0.45 μm of filtering with microporous membrane
To 44g compound flower water extracts.
Comparative example 7
By 30g peonies, 30g lotus flowers, 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g flower of Panax ginseng, 5g
HERBA DENDROBII orchid and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture through 70%
2450mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 52g compound flowers
Alcohol extracting thing；By another mixture through 70 DEG C of water extraction 120min of 2450mL water, filtering, added after being condensed into 300mL solution
900mL 95% ethanol is added after concentration, is stood overnight, filtrate filtrate freeze-drying, obtains through 0.45 μm of filtering with microporous membrane
To 48g compound flower water extracts.
Comparative example 8
By 30g peonies, 30g lotus flowers, 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 5g gold
Hairpin stem of noble dendrobium orchid and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by a mixture through 70%
2450mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 43g compound flowers
Alcohol extracting thing；By another mixture through 70 DEG C of water extraction 120min of 2450mL water, filtering, added after being condensed into 300mL solution
900mL 95% ethanol is added after concentration, is stood overnight, filtrate filtrate freeze-drying, obtains through 0.45 μm of filtering with microporous membrane
To 38g compound flower water extracts.
Comparative example 9
By 30g peonies, 30g lotus flowers, 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 30g people
Ginseng flower and the mixing of 30g sweet osmanthus, obtain mixture, two parts of said mixtures are prepared, by second of a mixture through 70% 2700mL
Alcohol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 55g compound flower alcohol extracting things；
By another mixture through 70 DEG C of water extraction 120min of 2700mL water, filtering, addition 1080mL 95% ethanol, quiet after concentration
Put overnight, filtrate filtrate freeze-drying, obtains 50g compound flower water extracts through 0.45 μm of filtering with microporous membrane.
Comparative example 10
By 30g peonies, 30g lotus flowers, 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 30g people
Ginseng flower, the mixing of 5g HERBA DENDROBIIs orchid, obtain mixture, two parts of said mixtures are prepared, by a mixture through 70%
2450mL ethanol, 500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 50g compound flowers
Alcohol extracting thing；By another mixture through 70 DEG C of water extraction 120min of 2450mL water, filtering, added after being condensed into 300mL solution
900mL 95% ethanol is added after concentration, is stood overnight, filtrate filtrate freeze-drying, obtains through 0.45 μm of filtering with microporous membrane
To 46g compound flower water extracts.
Comparative example 11
30g lotus flowers, by 30g roses, 30g BAISHACHAs flower, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 5g HERBA DENDROBII orchids
Mixed with 30g sweet osmanthus, obtain mixture, prepare two parts of said mixtures, by ethanol of a mixture through 70% 2150 mL,
500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 37g compound flower alcohol extracting things；Will be another
A mixture filtering, adds 860mL 95% ethanol, stood through 70 DEG C of water extraction 120min of 2150mL water after concentration
Night, filtrate filtrate freeze-drying, obtain 34g compound flower water extracts through 0.45 μm of filtering with microporous membrane.
Comparative example 12
By 30g peonies, 30g lotus flowers, 30g Jasmines, 30g peach blossoms, 30g plum blossoms, 30g flower of Panax ginseng, 5g HERBA DENDROBIIs orchid and
30g sweet osmanthus mixes, and obtains mixture, prepares two parts of said mixtures, by ethanol of a mixture through 70% 2100 mL,
500W ultrasonic extraction twice, each 30min；Filtrate merges, and rotary evaporation concentration, obtains 40g compound flower alcohol extracting things；Will be another
A mixture filtering, adds 860mL 95% ethanol, stood through 2100 mL water, 70 DEG C of water extraction 120min after concentration
Night, filtrate filtrate freeze-drying, obtain 36g compound flower water extracts through 0.45 μm of filtering with microporous membrane.
Embodiment 4
The compound flower alcohol extracting thing and compound flower water extract that Example 1 ~ 3 and comparative example 1 ~ 12 are prepared, are used respectively
DMSO or water are diluted to 0.1mg/mL, take sample solution 2ml to add in test tube, add 76 μm of ol/L DPPH ethanol of 2ml
Solution, mix, lucifuge reacts 30min at dark at room temperature, and absorbance A bs is determined at 525nm；According to formula below
Clearance rate is calculated, as a result as shown in table 1.
Clearance rate I (%)=[1- (T-T0)/(C-C0)] × 100%
In formula： T0：The absorbance of 0.1ml water or dimethyl sulfoxide (DMSO) plus 2 ml DPPH solution；
T：0.1ml sample liquids add the absorbance of 2 ml DPPH solution；
C0：The absorbance of 0.1ml water or dimethyl sulfoxide (DMSO) plus the ethanol of 2ml 95%；
C：0.1ml sample liquids add the absorbance of the ethanol of 2ml 95%.,
The compound flower extract DPPH inhibiting rates that the embodiment of the present invention of table 1 and comparative example are prepared
| Alcohol extract DPPH inhibiting rates（%） | Water extract DPPH inhibiting rates（%） | |
| Embodiment 1 | 97 | 95 |
| Embodiment 2 | 98 | 97 |
| Embodiment 3 | 98 | 97 |
| Comparative example 1 | 75 | 70 |
| Comparative example 2 | 75 | 71 |
| Comparative example 3 | 75 | 70 |
| Comparative example 4 | 76 | 72 |
| Comparative example 5 | 81 | 75 |
| Comparative example 6 | 77 | 74 |
| Comparative example 7 | 76 | 72 |
| Comparative example 8 | 83 | 76 |
| Comparative example 9 | 78 | 71 |
| Comparative example 10 | 75 | 70 |
| Comparative example 11 | 60 | 55 |
| Comparative example 12 | 68 | 60 |
Embodiment 5
The compound flower alcohol extracting thing and compound flower water extract that Example 1 ~ 3 and comparative example 1 ~ 12 are prepared, use
The modification methods such as Hosoi determine extract to melanin inhibitory action, test concentrations are 0.1 mg/mL.B16 melanocytes
With 1 × 105Density culture changes liquid in 96 orifice plates after 24h, adds the medicine of various concentrations, and after 72h, two are washed through PBS
Secondary, sample is dissolved in 200 μ l 1N NAOH (containing 1%DMSO), cooled down after heated 80 DEG C to 1h, select 475nm through being air-dried
Wavelength reads absorbance on enzyme-linked immunosorbent assay instrument.Melanin content inhibiting rate result such as table 2 is calculated according to formula below
It is shown.
Melanin content inhibiting rate=[1- (medicine hole absorbance/medicine hole cell density) (control wells absorbance/
According to hole cell density)] × 100%
Compound flower extract tyrosinase inhibition rate and the melanin content suppression that the embodiment of the present invention of table 2 and comparative example are prepared
Rate processed
Embodiment 6
The compound flower alcohol extracting thing and compound flower water extract that Example 1 ~ 3 and comparative example 1 ~ 12 are prepared, are added to
In human fibroblasts nutrient solution, using the deionized water without sample as blank control, final concentration of 0.1 mg/mL is tested.Training
After supporting 48 hours, with mtt assay to cell dyeing after, with absorbance at ELIASA measure 550nm, the proliferation rate of blank control is
100%, reference blank control, assess the proliferation function to human fibroblasts；Cell conditioned medium sample is taken simultaneously, with type i collagen egg
The generation of white measure kit measurement collagen, the production rate of blank control is 100%, reference blank control, assess to people into
The type i collagen synthesis facilitation of fibrocyte, as a result as shown in table 3.
The fibroblast proliferation rate and I types for the compound flower extract that the embodiment of the present invention of table 3 and comparative example are prepared
Collage synthesis rate
Described above is only the preferred embodiment of the present invention, it is noted that is come for those skilled in the art
Say, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
- A kind of 1. compound flower extract, it is characterised in that by peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, Plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus obtain through solvent extraction.
- 2. compound flower extract according to claim 1, it is characterised in that the peony, lotus flower, rose, Bai Shan Camellia, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and the mass ratio of sweet osmanthus are（1~5）:（1~5）:（1~5）:（1 ~5）:（1~5）:（1~5）:（1~5）:（1~5）：（0.1~1）:（1~5）.
- 3. compound flower extract according to claim 1, it is characterised in that the solvent is ethanol or water.
- 4. a kind of preparation method of compound flower extract, including：By peony, lotus flower, rose, BAISHACHA flower, Jasmine, peach blossom, plum blossom, flower of Panax ginseng, HERBA DENDROBII orchid and sweet osmanthus Mixing, obtains mixture, mixture is obtained into compound flower extract through solvent extraction.
- 5. the preparation method of compound flower extract according to claim 4, it is characterised in that the solvent is ethanol, institute It is the ethanol water that volumn concentration is 10% ~ 80% to state ethanol.
- 6. the preparation method of compound flower extract according to claim 5, it is characterised in that the quality g of the mixture Ratio with the volume mL of ethanol is 15 ~ 25:150~250.
- 7. the compound flower described in compound flower extract or claim 4 ~ 6 any one described in claim 1 ~ 3 any one Application of the compound flower extract that the preparation method of extract is prepared in free radical scavenger is prepared.
- 8. the compound flower described in compound flower extract or claim 4 ~ 6 any one described in claim 1 ~ 3 any one Application of the compound flower extract that the preparation method of extract is prepared in tyrosinase inhibitor is prepared.
- 9. the compound flower described in compound flower extract or claim 4 ~ 6 any one described in claim 1 ~ 3 any one Application of the compound flower extract that the preparation method of extract is prepared in fibrocyte proliferation accelerator is prepared.
- 10. food/pharmaceutical/cosmetics of a kind of whitening, it is characterised in that including as described in claim 1 ~ 3 any one The compound flower that the preparation method of compound flower extract described in compound flower extract or claim 4 ~ 6 any one is prepared Extract.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610277799.8A CN107334944A (en) | 2016-04-29 | 2016-04-29 | Compound flower extract and preparation method thereof |
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| CN201610277799.8A CN107334944A (en) | 2016-04-29 | 2016-04-29 | Compound flower extract and preparation method thereof |
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| CN115737495A (en) * | 2022-10-26 | 2023-03-07 | 现代百朗德生物科技(江苏)有限公司 | Siji flower essence extracting solution with anti-inflammatory, antioxidant, whitening and anti-wrinkle effects and preparation method thereof |
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| CN112106865A (en) * | 2020-09-30 | 2020-12-22 | 湖北友花茶业科技有限公司 | Preparation method of wintersweet flower extract |
| CN115737495A (en) * | 2022-10-26 | 2023-03-07 | 现代百朗德生物科技(江苏)有限公司 | Siji flower essence extracting solution with anti-inflammatory, antioxidant, whitening and anti-wrinkle effects and preparation method thereof |
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Application publication date: 20171110 |