CN113633591B

CN113633591B - Whitening composition, preparation method and application

Info

Publication number: CN113633591B
Application number: CN202111038330.6A
Authority: CN
Inventors: 曹茜; 周秋娜; 金荣熙; 吕凤; 姚清; 尹锺赫; 金延埈
Original assignee: Cosmax China Cosmetics Co Ltd
Current assignee: Cosmax China Cosmetics Co Ltd
Priority date: 2021-09-06
Filing date: 2021-09-06
Publication date: 2023-06-09
Anticipated expiration: 2041-09-06
Also published as: CN113633591A

Abstract

The invention provides a whitening composition, a preparation method and application. The composition is prepared from rhodiola rosea fermentation product extract, daisy extract and lansium leaf extract by the processes of crushing, fermenting, extracting, concentrating, formulating, sterilizing and the like, has good synergistic effect, can influence the production process of tyrosinase, inhibit the activity of tyrosinase, reduce the production of melanin and block the transfer of melanin in keratinocytes, thereby achieving the effect of effectively whitening. The extract has mild physical properties, is easy to absorb, has no irritation, is especially suitable for people to make up, and can be widely used in skin care cosmetics.

Description

Whitening composition, preparation method and application

Technical Field

The invention belongs to the field of cosmetics, and relates to a whitening composition, a preparation method and application.

Background

The epidermis is formed by basal layer development, and the basal layer is composed of basal cells and melanocytes, and the melanocytes produce melanin, which is the basis of pigment synthesis in the human pigment system. The melanin produced in the cells is transported into the stratum corneum cells via dendrites. It is prepared by the following steps of 1:36 proportion and keratinocyte, which form an epidermis unit, are present in the epidermis cell layer, and melanocytes form skin color by synthesis of nuclides, and absorb ultraviolet rays, so that the body is protected from the damage of ultraviolet rays. The shade of skin color is determined by how much melanin is synthesized by melanocytes.

Whitening is a major hot spot in cosmetic skin care and has long received widespread attention from asian women. In recent years, the attention of consumers to the efficacy of whitening and freckle-removing skin care products is continuously rising, so that a large number of whitening and freckle-removing products are emerging in the market. However, at present, the whitening cosmetics at home and abroad have some problems, such as poor whitening effect. And the cosmetics with good whitening effect often contain hydroquinone and mercury salt, and have great toxic and side effects, and can cause permanent fading of skin and contact dermatitis after long-term use. There is a need to find some natural and safe and effective whitening products.

To prevent melanin deposition, the following two methods are generally adopted: first, inhibiting melanin production prevents the formation of pigmented spots by inhibiting the production of a complex amylase and the activity of a complex amylase, or interfering with intermediates of melanin production. Second, reduction of melanin and prevention of photooxidation. The produced melanin is desalted by stimulating the reduction of melanin by keratinocytes. The invention is intended to be carried out by the synchronization of the two modes of action. The transfer of melanin chains is blocked, so that the effect of effective whitening is achieved.

Rhodiola rosea is a rare medicinal plant growing in northern hemisphere alpine and pollution-free areas. Is a perennial herb plant, and is 10-20 cm high. The root is thick, conical, fleshy, brown and yellow, the root neck has a plurality of fibrous roots, short rootstock, thick and cylindrical, and is covered by a plurality of scaly leaves which are arranged in a tile shape. The plant grows in a high-cold pollution-free zone with an altitude of 1800-2500 m, and has a severe growth environment, thus having strong vitality and special adaptability. Can be used as medicine, can tonify qi, clear lung, promote intelligence and nourish heart, and is a traditional Chinese medicine with wide functions. Also has great beautifying effect, and can be used as skin care product or edible product. The rhodiola rosea plants are fermented by the saccharomycetes, so that all functional components and the activity of the plants can be reserved, the loss of active components caused by a chemical extraction method is avoided, and the rhodiola rosea plants and the active components in the fermentation filtrate have a synergistic effect.

The english "day" of the daisy is derived from "daeseage" or "diurnal eyes" in the young's luffa, and means a manner in which such flowers open and close with sunrise and sunset. The Latin names "bellis perennis" are derived from "bellus" (beauty) and "perennis" (durability), and today, daisy is one of the most widely planted family flowers, and is a national flower of Italy. Is a perennial or annual pepperweed herb, and is about 10 cm high. She Jisheng the grass, spoon-shaped, rounded at the tip, tapered at the base to a handle, with blunt or wavy teeth on the upper edge. The daisy contains abundant essential oil, chrysanthemum pigment, amino acid and various microelements, wherein the content of flavonoid components is 32% to 61% higher than other chrysanthemums. Has good whitening effect and is widely applied to various whitening products.

Lanceolata fruit trees (Laium domestum), which are typical tree species in southeast Asia, are sweet and fragrant fruits from Thailand peninsula to eastern Veronica, and are very popular desserts. In some places, the pericarp is used for treating diarrhea and enterospasm, has good antioxidant activity, can treat fever by grinding seeds, can be orally taken as diarrhea and malaria by bark, can be used as a raw material of cosmetics after leaf extraction, and realizes effective utilization of resources. The miR-490-3p can control the synthesis process of tyrosinase in melanocytes after being paired with tyrosinase mRNA, so that the production of tyrosinase is reduced. Studies have shown that Lane fruit leaf extracts can promote the increase of miR-490-3 p.

The existing raw materials with the whitening effects of cosmetics on the market have the problems of single target point and difficult permeation and absorption, and even part of the raw materials have the problems of poor solubility and poor stability. The rhodiola rosea, the lansium leaves and the daisy are selected as three natural raw materials, the rhodiola rosea extract and the hydrolysate thereof can inhibit the phosphorylation of C-AMP response element binding protein (CREB), inhibit the expression of related transcription factor (MITF) and tyrosinase related protein 1 (TRP-1), and play a role by regulating melanin CREB/MITF/tvrosinase generation channels; flavonoid components in the young chrysanthemum can effectively inhibit the expression of ET-1 in keratinocytes, block a receptor MC-1 (melanocortin receptor 1) in melanocytes and inhibit the transfer of melanin in the keratinocytes; and miR-490-3p is a segment of miRNA highly complementary to tyrosinase mRNA, and can exert synergistic effect with rhodiola rosea extract and daisy extract in a melanin CREB/MITF/tvrosinase generation path, and the lansium fruit leaf extract can promote the increase of miR-490-3 p.

Disclosure of Invention

The invention scientifically proportions the rhodiola rosea product extract, the daisy extract and the lansium leaf extract which are fermented by saccharomycetes aiming at various ways of melanin generation and transfer, on one hand, the generation process of tyrosinase is influenced by blocking the transcription of protein, and on the other hand, the combination of tyrosinase and a substrate can be blocked by two modes of competitiveness and non-competitiveness, the activity of tyrosinase is inhibited, and the transfer of melanin in keratinocytes is blocked, so that the effect of effectively whitening is achieved.

In order to achieve the above purpose, the application provides a whitening composition, which is prepared by mixing the following components in percentage by mass: comprises rhodiola rosea fermentation product extract, daisy extract and lansium fruit leaf extract= (5-15): (2-5): (0.1-0.5).

Preferably, the rhodiola rosea fermentation product extract: extract of marguerite: lansium leaf extract = 10:2:0.5 or 5:3:0.5 or 10:2:0.3.

preferably, the whitening composition is prepared by the following method:

(1) Respectively pulverizing radix Rhodiolae, herba Belleville and Lansium leaf;

(2) Adding deionized water 5-10 times of the crushed rhodiola rosea, the daisy and the Lansii leaf powder respectively, and soaking for 1-3 hours; adding cellulase at 40-50deg.C, stirring for 20 min, heating to 40-60deg.C, microwave treating for 5-15 min, reflux extracting in ultrasonic water bath for 1-3 hr, and repeating for 2-3 times to obtain radix Rhodiolae solution, marguerite solution and Lansii leaf solution;

(3) Adding 1-3 times of 85-95% ethanol solution into radix Rhodiolae solution, marguerite solution and Langshui fruit leaf solution, standing at low temperature for 8-10 hr, concentrating by rotary evaporator to obtain radix Rhodiolae concentrate, marguerite concentrate and Langshui fruit leaf concentrate;

(4) Adding 3-5 times deionized water, 0.5-1.5 times phosphate buffer solution with pH of 7-8 and concentration of 0.35mmol/L, and 1-3 times glucose aqueous solution with concentration of 30-50wt% into radix Rhodiolae concentrated solution to obtain fermentation substrate; fermenting with yeast, centrifuging, and collecting supernatant;

(5) Adsorbing the supernatant obtained in the step (4) by using cation exchange resin, and eluting until the supernatant is colorless to obtain a rhodiola rosea fermentation product extract;

(6) Adding 3-5 times deionized water into the concentrated solution of Bellis Persica and concentrated solution of Lansium, sequentially extracting with petroleum ether, chloroform and ethyl acetate, shaking vigorously for 2-4 hr, separating organic layer, extracting for 2-3 times until colorless, and mixing ethyl acetate layers; concentrating the ethyl acetate organic layer to obtain fluid extract of Belleville extract and fluid extract of Lansium fruit leaf extract respectively;

(7) Dissolving the extract obtained in the step (6) with 1-3 times of deionized water respectively, adding a clarifying agent, standing, centrifuging for 30-60 minutes, filtering with a microfiltration membrane, and adding 3 times of maltodextrin for full dissolution; filtering, concentrating under reduced pressure to obtain crude extract of Belleville extract and crude extract of Lansium fruit leaf extract respectively;

(8) Filtering the crude extract obtained in the step (7) by using an ultrafiltration membrane respectively, and collecting filtrate to obtain a daisy extract and a lansium leaf extract;

(9) And (3) combining the extracts obtained in the step (5) and the step (8) according to a proportion to obtain the whitening composition.

Further preferred is:

the concentration in the step (3) means concentration to 1/2-1/5 of the original weight;

the concentration in the step (7) means concentration to 1/3 of the original weight;

the aperture of the microfiltration membrane in the step (7) is 0.1-1.0 mu m;

the aperture of the ultrafiltration membrane in the step (8) is 0.01-0.05 mu m.

Further preferably, the cellulase is added in an amount of 10% -20% of the total mass of the raw material.

Further preferably, the addition amount of the saccharomycetes is 1-2% of the mass of the fermentation substrate, the fermentation temperature is 25-37 ℃, and the fermentation time is 5-10 days.

In another aspect, the invention provides a cosmetic comprising the composition and a cosmetically acceptable adjuvant.

Preferably, the composition accounts for 0.5-5% of the weight of the cosmetic.

Preferably, the cosmetic is water, emulsion, spray, cream, gel or facial mask.

According to the invention, a plurality of ways of melanin generation and transfer are aimed at, and the rhodiola rosea product extract, the lansium leaf extract and the daisy extract are scientifically proportioned, so that on one hand, the generation process of tyrosinase is influenced by blocking the transcription of protein, and on the other hand, the combination of tyrosinase and a substrate can be blocked in a competitive mode and a non-competitive mode, the activity of tyrosinase is inhibited, and the transfer of melanin in keratinocytes is blocked, so that the effect of effectively whitening is achieved.

Drawings

FIG. 1 is a graph comparing the effects of different compositions on melanin transfer in experimental example 4;

FIG. 2 is a graph comparing the effect of different compositions on stain reduction in Experimental example 6;

FIG. 3 is a graph comparing the effect of different compositions on skin tone in Experimental example 6.

Detailed Description

Hereinafter, the present invention will be described in more detail and in detail with reference to examples, but the following examples are not intended to limit the present invention. In the present invention, all the equipment and raw materials are commercially available or commonly used in the industry, and the methods in the following examples are conventional in the art unless otherwise specified. The present invention is not limited to the specific embodiments, and any person skilled in the art can easily think about the changes and substitutions within the technical scope of the present invention, and the changes and substitutions are intended to be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Example 1: preparation of whitening composition with multi-mode action

In the following examples and comparative examples of the present invention, a multi-mode whitening composition was prepared using the following process.

(2) Adding 5 times of deionized water into the crushed rhodiola rosea, the daisy and the Lansii leaf powder respectively, and soaking for 3 hours. Adding cellulase at 48 ℃ in water bath, stirring for 20 minutes, heating to 54 ℃ and carrying out microwave treatment for 5-15 minutes, and carrying out ultrasonic water bath reflux extraction for 3 hours, wherein the total mass of the added cellulase accounts for 10% of the total mass of the raw materials, and the solution A (rhodiola rosea), the solution B (daisy) and the solution C (lansium fruit leaves) are obtained;

(3) Adding 90% ethanol solution into the solutions A/B/C2 times, standing at low temperature for 10 hr, and concentrating to 1/2 of the original weight by rotary evaporator to obtain concentrated solutions D (radix Rhodiolae), E (Bellis Persianae), F (Langshui fruit leaf);

(4) Adding 3 times deionized water, 1 time phosphate buffer solution with pH of 7-8 and concentration of 0.35mmol/L and 1-3 times glucose aqueous solution with concentration of 50wt% into the concentrated solution D in sequence to obtain fermentation substrate. Adding 2% yeast by mass into the fermentation substrate, culturing at room temperature for 10 days, centrifuging, and collecting supernatant;

(5) Adsorbing the supernatant in the step (4) by adopting cation exchange resin, and eluting until the supernatant is colorless to obtain a rhodiola rosea fermentation product extract;

(6) Adding 3 times of deionized water into the concentrated solution E/F, sequentially extracting with petroleum ether, chloroform and ethyl acetate, shaking vigorously for 2h, separating organic layers, extracting for 3 times until colorless, mixing ethyl acetate layers, concentrating under reduced pressure in a rotary evaporator, recovering ethyl acetate at room temperature to obtain fluid extract of Belleville and fluid extract of Lansium respectively;

(7) Dissolving the extract obtained in the step (6) with 3 times of deionized water respectively, adding clarifier, standing, centrifuging for 60 min, filtering with 1.0 μm microfiltration membrane, and adding 3 times of maltodextrin for dissolving completely. Filtering, concentrating under reduced pressure to 1/3 of the original weight to obtain crude extract of Belleville extract and crude extract of Lansium album leaf;

(8) Filtering the solution obtained in the step (7) by using a 0.05 mu m ultrafiltration membrane respectively, and collecting filtrate to obtain a daisy extract and a lansium leaf extract;

(9) And (3) combining the extracts obtained in the step (5) and the step (8) according to a proportion to obtain a series of whitening compositions capable of acting in multiple ways.

The compositions 1 to 15, comparative examples 1 to 3 were obtained by compounding in the proportions shown in Table 1 below.

TABLE 1

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Note that: to ensure equal concentrations at the time of addition, the total amount of addition at the time of preparation of each composition is the same here.

Example 2: test for inhibiting melanogenesis in murine melanoma cells

In the above-described compositions and comparative examples of the present invention, the inhibition test method and results of the extract composition on melanin production are as follows:

taking mouse melanoma B16 cells in logarithmic growth phase, inoculating the cells into a T25 cell culture flask, and culturing overnight. Samples were added to the cells in an untreated group (without adding any sample) as a cell control group, with a volume fraction of 1% (diluted with deionized water). After 48h incubation, washing 1 time with PBS, adding 1mL of 1mol/L NaOH solution, scraping the collected cells, placing in a 80 ℃ water bath for 30min, taking the supernatant, adding into a 96-well plate, and reading absorbance at 475nm by an M3 plate reader. The melanin content of the control group was 100%.

Relative melanin content (%) = (measured well absorbance value-blank absorbance value)/(cell control absorbance value-blank absorbance value) ×100%.

Experimental results: the results are shown in Table 2, and comparing compositions 1-15 with comparative examples 1-3, it was found that:

1) After the 3 raw materials are compounded and combined, the inhibition of melanin generation is superior to that of a single component;

2) The melanin production inhibition rates corresponding to different proportions of the 3 raw material compound combinations are different, and the melanin production inhibition rates are preferably rhodiola rosea fermentation product extract, daisy extract and lansium leaf extract= (5-15): (2-5): (0.1-0.5), more preferably rhodiola rosea fermentation product extract: extract of marguerite: lansium leaf extract = 10:2:0.5 or 5:3:0.5 or 10:2:0.3.

TABLE 2

The above test results show that the compositions of test examples 1 to 15 all have the effect of reducing melanin in melanoma cells of mice, but different proportions of the compositions have an effect on the reduction of melanin under the same conditions, and the melanin reduction effect of

compositions

4, 8 and 9 is relatively good.

Example 3: inhibition assay of tyrosinase activity in murine melanoma cells

Taking mouse melanoma B16 cells in logarithmic growth phase, inoculating the cells into a T25 cell culture flask, and culturing overnight. The samples were added in a volume fraction of 1% (deionized water dilution) and the untreated group (without any added samples) was used as a cell control group. After 48h incubation, washing 1 time with PBS, adding 1mL of 1mol/L NaOH solution, scraping the collected cells, placing in a 80 ℃ water bath for 30min, taking the supernatant, adding into a 96-well plate, and reading absorbance at 475nm by an M3 plate reader. Wherein the tyrosinase activity of the control group was 100%.

Relative tyrosinase activity (%) = (assay well absorbance value-blank absorbance value)/(cell control absorbance value-blank absorbance value) ×100%. The test results are shown in Table 3:

TABLE 3 Table 3

The above test results show that the compositions of test examples 1 to 15 all have the effect of inhibiting tyrosinase activity, but the inhibition ability of tyrosinase activity is also affected by different proportions of the compositions under the same conditions, wherein the inhibition of

compositions

4, 8 and 9 is relatively good. Thus, the rhodiola rosea fermentation product extract can be judged: extract of marguerite: the combination of the lansium leaf extract and the lansium leaf extract can effectively inhibit tyrosinase activity, thereby achieving the whitening effect.

Example 4: keratinocyte melanin transfer assay

Inoculating epidermal keratinocyte into culture flask containing DK-SFM culture solution, and placing in CO at 37deg.C ₂ (CO ₂ Volume fraction 5%) in a constant temperature incubator for 6d. Taking out the cell culture flask, adding trypsin into the cell culture flask to digest for 4min, inoculating the cells into a 96-well plate, incubating for 24h, adding 200 mu L of cell culture solution into each well of a control group, adding 200 mu L of different plant composition extracting solutions with mass concentration of 1% (diluted by deionized water) into each well of a medicine group, and culturing in an incubator for 48-72 h. Adopts the following steps of

(Molecular Probes) microbead model. Keratinocytes were stimulated with ultraviolet light to mimic the uptake of melanosomes by endocytosis. 100% absorbed cultures were used as control.

The results shown in FIG. 1 demonstrate that each composition is effective in reducing the amount of the composition compared to the culture (100% absorption) as a control (without any added sample)

Reduces melanosome transfer.

Example 5: human efficacy testing

In a specific embodiment, the invention provides a formula process of facial mask muscle base liquid containing a whitening extract composition with multi-mode action, and the specific formula is shown in table 4:

TABLE 4 Table 4

The extract of claim is added, and the

compositions

4, 8 and 9 are added into the formula of the lower surface membrane muscle base solution, wherein the addition amount is 1%, so that the mask muscle base solution samples 1-3 are prepared.

Composition 4	Composition 8	Composition 9
			Sample 1	Sample 2	Sample 3

Example 6

1. MTT cytotoxicity test

In 96well Multiplate (burning) according to 1X10 ⁴ The density of cells/well was inoculated with 100. Mu.L each of DMEM medium containing 10% bovine serum and keratinocytes (HaCaT), and cultured for 24 hours and then replaced with serum-free medium.

Compositions

4, 8, and 9 of the above examples (diluted with deionized water) were added to serum-free medium, respectively, and incubated for 24 hours. The medium was then removed, treated with 20. Mu.L of MTT solution and allowed to react at 37℃for 2 hours. 200 mu L of isopropanol was added to the MTT solution-removed cells, gently swirled for 30min, the crystalline formazan was completely dissolved, absorbance was measured at 570nm, and cell viability was calculated according to the following formula.

The control group was tested without the addition of the composition. Cytotoxicity related results are shown in table 5 below:

TABLE 5

2. Safety test (human body skin patch test)

15 healthy subjects with no history of dermatological allergy between 20 and 50 years of age were selected, and the patch method: selecting qualified plaque testers, dripping about 15 mu L of sample 1-3 into the plaque testers in a closed plaque test mode, sticking the sample on the back of a subject by using a special adhesive tape, sticking 20 plaque testers to each subject, respectively sticking the myobase fluid sample of the sample 1-3, removing the testers after 24 hours, observing skin reactions after removing the testers for 0.5, 6, 12, 24 and 48 hours, and recording the results according to skin reaction grade standards in skin care product health Specification.

Test results: the results of the human skin patch test show that all subjects pass the patch test and observe skin reactions at 0.5, 6, 12, 24 and 48 hours, wherein adverse reactions such as skin erythema, pimple and blister appear in 0 cases, which indicates that the product of the invention is safe and has no stimulation.

3. Stain lightening test

Test sample: sample 1/2/3

Test instrument: MEXAMETER MX18 skin pigment instrument

Measurement environment: testing ambient temperature (21+ -1deg.C, humidity (50+ -5))

The testing method comprises the following steps: 45 female volunteers 18-60 years old were selected and divided into three groups, and their melanin content of facial skin stain was measured before using the test samples and after 28 days of continuous use of the cosmetics. The test subjects used 2 times per day of essence at the selected part, cleaned the facial skin, wiped the skin, smeared the essence on the facial skin by hands, and used for four weeks continuously.

The subject did not use other whitening products during the test. The higher the measured value, the higher the melanin content in the skin. Melanin reduction rate = (melanin content before use of essence-melanin content after continuous use of essence for 28 days)/melanin content before use of essence x 100%. The higher the melanin reduction rate, the more remarkable the stain-lightening effect. The data were averaged and shown in figure 2.

As can be seen from fig. 2, the combination of the rhodiola rosea fermentation product extract, the daisy extract and the lansium leaf extract can lighten the color spot melanin and exert better skin-color-uniform whitening effect.

4. Skin color lightening test

Test instrument: skin gloss tester SkinGlossMeter

The testing method comprises the following steps: testing ambient temperature: 21±1 ℃, relative humidity: 50+ -5%

45 female volunteers 18-60 years old are selected and divided into three groups, and skin glossiness of the subjects before and after facial skin is tested by using the essence prepared by the invention through a skin glossiness tester SkinGlossMeter. The skin surface gloss is reflected by direct and diffuse reflection of light striking the skin surface, with higher values indicating a shinier skin. The data were averaged. The results of this experiment are shown in FIG. 3.

As can be seen from fig. 3, the skin glossiness of the subject is increased after using sample 1/2/3 compared with that before using, so that the three combinations can be judged to have the effect of improving skin glossiness.

What is not described in detail in the present specification is common general knowledge of a person skilled in the art.

As used throughout the specification and claims, the word "comprise" is an open-ended term, and thus should be interpreted to mean "include, but not limited to. By "substantially" is meant that within an acceptable error range, a person skilled in the art is able to solve the technical problem within a certain error range, substantially achieving the technical effect.

It should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a product or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such product or system. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a commodity or system comprising such elements.

While the foregoing description illustrates and describes the preferred embodiments of the present application, it is to be understood that this application is not limited to the forms disclosed herein, but is not to be construed as an exclusive use of other embodiments, and is capable of many other combinations, modifications and environments, and adaptations within the scope of the inventive concept described herein, through the foregoing teachings or through the skill or knowledge of the relevant arts. And that modifications and variations which do not depart from the spirit and scope of the present invention are intended to be within the scope of the appended claims.

Claims

1. The whitening composition is characterized by being prepared by mixing the following components in parts by mass: rhodiola rosea fermentation product extract, daisy extract and lansium fruit leaf extract= (5-15): (2-5): (0.1-0.5);

the whitening composition is prepared by the following method:

(1) Respectively pulverizing radix Rhodiolae, lansiguo leaf and Belleville;

(2) Adding deionized water 5-10 times of the crushed rhodiola rosea, the daisy and the Lansii leaf powder respectively, and soaking for 1-3 hours; adding cellulase in water bath at 40-50deg.C, stirring for 20 min, heating to 40-60deg.C, microwave treating for 5-15 min, reflux extracting in ultrasonic water bath for 1-3 hr, and repeating for 2-3 times to obtain radix Rhodiolae solution, marguerite solution and Lansii leaf solution;

2. The whitening composition according to claim 1, wherein the rhodiola rosea fermentation product extract: extract of marguerite: lansium leaf extract = 10:2:0.5 or 5:3:0.5 or 10:2:0.3.

3. the whitening composition according to claim 1, characterized in that:

the aperture of the microfiltration membrane in the step (7) is 0.1-1.0 mu m;

the aperture of the ultrafiltration membrane in the step (8) is 0.01-0.05 mu m.

4. The whitening composition according to claim 1, wherein the cellulase is added in an amount of 10 to 20% by weight of the total mass of the raw materials.

5. The whitening composition according to claim 1, wherein the yeast is added in an amount of 1-2% by mass of the fermentation substrate, the fermentation temperature is 25-37 ℃, and the fermentation time is 5-10 days.

6. A cosmetic product comprising a composition according to any one of claims 1 to 5 and a cosmetically acceptable adjuvant.

7. The cosmetic according to claim 6, wherein the composition is 0.5 to 5% by mass of the cosmetic.

8. The cosmetic product according to claim 7, wherein the cosmetic product is an aqueous solution, an emulsion, a spray, a cream, a gel or a mask.