CN104042548A - Snail grume extract as well as preparation method and application thereof in cosmetics - Google Patents
Snail grume extract as well as preparation method and application thereof in cosmetics Download PDFInfo
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Abstract
The invention discloses snail grume extract which contains 0.1-5% of chondroitin sulfate, 0.1-5% of allantoin and 0.1-10% of collagen, wherein the molecular weight distribution of micro-molecule polypeptide is within 3000-5500 Dalton. The invention further discloses a preparation method and an application of the snail grume extract in cosmetics. The snail grume extract has the advantages that high pressure homogenate extraction and multi-stage membrane separation coupling techniques are adopted, the effective components of the snail grume are highly enriched, the snail grume extract contains 0.1-5% of chondroitin sulfate, 0.1-5% of allantoin and 0.1-10% of collagen, and particularly the molecular weight distribution of micro-molecule polypeptide is within 3000-5500 Dalton, so that the snail grume extract is very applicable to human body skin absorption, is beneficial for injured skin remediation and can promote heal and accelerate cell regeneration when being added into cosmetics.
Description
Technical field
The present invention relates to a kind of Limax mucus extract and application thereof, particularly a kind of Limax mucus extract and preparation method thereof and the application in cosmetics.
Background technology
Cepaea Gastropoda terrestrial mollush, has very high edible and medical value.There is white jade snail in the China generally kind of cultivation, mainly contains collagen protein, allantoin, chondroitin sulfate, vitamin, natural antibacterial peptide isoreactivity composition in its mucus.Wherein allantoin can make skin regeneration; Protein and vitamin can increase the nutrition of skin, make skin finer and smoother and smooth.
At present, the application and development majority of Limax product being concentrated on to foods and cosmetics field, is mainly the extraction aspect of polysaccharide, Snailase, secretions to its research, but reports there are no the research of cosmetics-stage Limax mucus extract.
" comparative study of Jiangxi bar Limax extraction process of active component " that < < tropical disease and parasitology > > the 3rd the 3rd phase of volume in 2005 publishes reported a day snail polysaccharide extracting method." research of Snailase and application " that < < China's biochemical drug magazine > > the 2nd phase in 1993 publishes reported the method for extracting Snailase from Limax digestive gland.Application number is the extracting method that the Chinese invention patent application of CN201210292779.X discloses a kind of Limax mucus, adopts moment electric shock Limax foot, collects the mucus of Limax pedal gland secretion, then by lyophilization, obtains Limax secretions.
At present to the most extractions that concentrate on the aspects such as polysaccharide, Snailase, Limax mucus of Limax research report, have no the research report of preparation technology aspect of the cosmetics-stage Limax mucus extract of compositions such as containing collagen protein, allantoin, chondroitin sulfate, micromolecule polypeptide, the research of also evaluating aspect the efficacy effect in cosmetic applications and safety there are no Limax mucus extract.Application that can be in cosmetics in view of Limax mucus extract is more and more extensive, is necessary to study with regard to active component, the safety of the Limax mucus extract for used for cosmetic, with effect and the safety of guaranteeing that Limax extract is applied in cosmetics.
Summary of the invention
The shortcoming that the object of the invention is to overcome prior art, with not enough, provides a kind of being applied in cosmetics to be conducive to repair impaired skin, promotes healing, accelerates the Limax mucus extract of cell regeneration.
Another object of the present invention is to provide a kind of preparation method of Limax mucus extract.
The present invention's the 3rd object is to provide the application of a kind of Limax mucus extract in cosmetics.
To achieve these goals, the present invention is achieved through the following technical solutions: a kind of Limax mucus extract, wherein contain chondroitin sulfate 0.1～5%, allantoin 0.1～5%, collagen protein 0.1～10%, the molecular weight distribution of micromolecule polypeptide is 3000～5500 dalton, being very suitable for human body skin absorbs, be added in cosmetics and be conducive to repair impaired skin, promote healing, accelerate cell regeneration.
Described Limax mucus extract extracts and makes from white jade snail mucus, mainly contains collagen protein, allantoin, chondroitin sulfate, micromolecule polypeptide active component.
Described Limax mucus extract makes by following steps: after getting Limax mucus and mixing homogeneously with deionized water, with extracting in high pressure homogenizer; Extract material after centrifuge separation, obtain limpid extracting solution; Extracting solution is processed through microfiltration, except degerming, the impurity such as microorganism makes bacteria-removing liquid; Bacteria-removing liquid ultra-filtration and separation, removes the human body skins such as macromolecular albumen, polysaccharide and is difficult to the material absorbing; And then concentrated with nanofiltration, obtain the Limax mucus extract for cosmetics.
A preparation method for Limax mucus extract, it comprises the following steps:
(1) extract: get Limax mucus, add the water of 5～30 times of its quality, under 5～60 ℃ of temperature and 40～100Mpa pressure, carry out high-pressure homogenization historrhexis and extract 1～3 time.
(2) centrifugal: the high-pressure homogenization material of (1) step gained is separated with centrifuge, collect clear liquid.
(3) microfiltration: clear liquid is the microfiltration of ceramic membrane of 0.1 μ m-0.5 μ m with aperture, except degerming, the impurity such as microorganism;
(4) ultrafiltration: microfiltration dialysis solution molecular cut off is 6,000～50,000 daltonian ultrafilter membrane ultrafiltration, removes the human body skins such as macromolecular albumen, polysaccharide and is difficult to the material absorbing;
(5) nanofiltration: ultrafiltration dialysis liquid is concentrated by molecular cut off 80～2,000 daltonian NF membrane, collects concentrated solution, obtains Limax mucus extract, concentrates the Limax mucus extract obtaining for cosmetics by nanofiltration.
The mucus that the preferred white jade snail of described Limax mucus produces.
The application of Limax mucus extract in cosmetics, its addition is 0.5-20%.
An anti-apolexis composition, it comprises following parts by weight component:
An anti-ageing face mask, it comprises following parts by weight component:
The allantoin that Limax mucus extract contains can be repaired impaired skin, promotes healing, accelerates cell regeneration; Collagen protein has maintenance moisture, strengthens the effect of skin elasticity; Chondroitin sulfate is conducive to remove old cutin, reduces wrinkle of skin and cicatrix; Boy's polypeptide has stimulates skin regeneration, desalination mottle, promote the effect of skin quality and glossiness.
Compared with prior art, advantage of the present invention is: adopted high-pressure homogenization to extract multiple techniques separated with Using Multistage Membranes, Limax mucus effective ingredient is highly enriched, and Limax mucus extract contains chondroitin sulfate 0.1～5%, allantoin 0.1～5%, collagen protein 0.1～10%, particularly the molecular weight distribution of micromolecule polypeptide is 3000～5500 dalton, is very suitable for human body skin and absorbs, and is added on and in cosmetics, is conducive to repair impaired skin, promote healing, accelerate cell regeneration.
Accompanying drawing explanation
Fig. 1 is invention technical matters flow chart: represented major technique process of the present invention, wherein high-pressure homogenization extraction is key problem in technology of the present invention in conjunction with multistage membrane separation concentration;
Fig. 2 is product molecular weight distribution figure: represent, for the molecular weight distribution situation of polypeptide in the Limax mucus extract of used for cosmetic, to be mainly distributed in 3000～5500 dalton;
Fig. 3 is product cell increment experimental result picture: represent to have added the Limax mucus extract cell proliferation experiment result for used for cosmetic;
Fig. 4 is the blank group of product cell increment experimental result picture: the cytothesis experimental result that represents blank group.
The specific embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
A Limax mucus extract, wherein contains chondroitin sulfate 0.2% (mass percentage content), allantoin 0.2%, and collagen protein 0.2%, the molecular weight distribution of micromolecule polypeptide is 3100 dalton.
Embodiment 2
A Limax mucus extract, wherein contains chondroitin sulfate 4.9%, allantoin 4.9%, and collagen protein 9.9%, the molecular weight distribution of micromolecule polypeptide is 5400 dalton.
Embodiment 3
A Limax mucus extract, wherein contains chondroitin sulfate 1～3%, allantoin 1～3%, and collagen protein 1～6%, the molecular weight distribution of micromolecule polypeptide is 3500～5000 dalton.
Embodiment 4
A kind of Limax mucus extract, wherein contain chondroitin sulfate 2.5%, allantoin 2.5%, collagen protein 5%, the molecular weight distribution of micromolecule polypeptide is 4500 dalton, is very suitable for human body skin and absorbs, and is added on and in cosmetics, is conducive to repair impaired skin, promote healing, accelerate cell regeneration.
Embodiment 5
The preparation method of above-mentioned Limax mucus extract, as shown in Figure 1, it comprises the following steps:
(1) extract: get Limax mucus, add the water of 6 times of its quality, under 8 ℃ of temperature and 50Mpa pressure, with high pressure homogenizer, carry out high-pressure homogenization historrhexis and extract 1～3 time.
(2) centrifugal: the high-pressure homogenization material of (1) step gained is separated with centrifuge, collect clear liquid; Centrifuge speed can be 500-1300 rev/min;
(3) microfiltration: clear liquid is the microfiltration of ceramic membrane of 0.2 μ m m with aperture, except degerming, the impurity such as microorganism;
(4) ultrafiltration: microfiltration dialysis solution molecular cut off is 6,500 daltonian ultrafilter membrane ultrafiltration, removes the human body skins such as macromolecular albumen, polysaccharide and is difficult to the material absorbing;
(5) nanofiltration: ultrafiltration dialysis liquid is concentrated by the daltonian NF membrane of molecular cut off 100, collects concentrated solution, obtains Limax mucus extract, concentrates the Limax mucus extract obtaining for cosmetics by nanofiltration.
Embodiment 6
The preparation method of above-mentioned Limax mucus extract, it comprises the following steps:
(1) extract: get Limax mucus, add the water of 29 times of its quality, under 59 ℃ of temperature and 99Mpa pressure, with high pressure homogenizer, carry out high-pressure homogenization historrhexis and extract 1～3 time.
(2) centrifugal: the high-pressure homogenization material of (1) step gained is separated with centrifuge, collect clear liquid.
(3) microfiltration: clear liquid is the microfiltration of ceramic membrane of 0.49 μ m with aperture, except degerming, the impurity such as microorganism;
(4) ultrafiltration: microfiltration dialysis solution molecular cut off is 49,000 daltonian ultrafilter membrane ultrafiltration, removes the human body skins such as macromolecular albumen, polysaccharide and is difficult to the material absorbing;
(5) nanofiltration: ultrafiltration dialysis liquid is concentrated by the daltonian NF membrane of molecular cut off 1900, collects concentrated solution, obtains Limax mucus extract, concentrates the Limax mucus extract obtaining for cosmetics by nanofiltration.
Embodiment 7
The preparation method of above-mentioned Limax mucus extract, it comprises the following steps:
(1) extract: get Limax mucus, add the water of 20 times of its quality, under 45 ℃ of temperature and 75Mpa pressure, with high pressure homogenizer, carry out high-pressure homogenization historrhexis and extract 2 times.
(2) centrifugal: the high-pressure homogenization material of (1) step gained is separated with centrifuge, collect clear liquid.
(3) microfiltration: clear liquid is the microfiltration of ceramic membrane of 0.3 μ m with aperture, except degerming, the impurity such as microorganism;
(4) ultrafiltration: microfiltration dialysis solution molecular cut off is 35,000 daltonian ultrafilter membrane ultrafiltration, removes the human body skins such as macromolecular albumen, polysaccharide and is difficult to the material absorbing;
(5) nanofiltration: ultrafiltration dialysis liquid is concentrated by the daltonian NF membrane of molecular cut off 1800, collects concentrated solution, obtains Limax mucus extract, concentrates the Limax mucus extract obtaining for cosmetics by nanofiltration.
Extraction and isolation technics that the present invention adopts, whole production process can be carried out under cryogenic conditions, has guaranteed that collagen protein, micromolecule polypeptide isoreactivity composition are not destroyed.Simultaneously owing to having adopted Using Multistage Membranes isolation technics, Limax mucus macromolecular substances and bacterial micro-organism have been removed, highly enriched collagen protein and micromolecule polypeptide, guarantee that Limax mucus extract is in the safety of cosmetic applications with at the efficacy effect aspect skin repair.
Wherein, the mucus that the preferred white jade snail of described Limax mucus produces.The preferred hollow-fibre membrane of described ultrafilter membrane, the ultrafilter membrane of described hollow-fibre membrane internal diameter between 0.6-6mm is called capillary type ultrafilter membrane, and capillary type ultrafilter membrane, because internal diameter is larger, is difficult for being stopped up by large particulate matter.Described NF membrane can be that polyamide material is made.
Embodiment 8
Get 1.0kg Limax mucus, be placed in and extract still, add the water of 30.0L, be heated to 60 ℃, after being uniformly mixed, with extracting in high pressure homogenizer 3 times.Resulting extraction material centrifuge centrifugalize, disgorging matter.Centrifugal liquid is that 0.5 μ m microfiltration of ceramic membrane is processed with aperture, the daltonian ultrafilter membrane ultrafiltration of molecular cut off 50,000 for microfiltration dialysis solution, ultrafiltration dialysis liquid is concentrated by molecular cut off 2,000 dalton's NF membrane, collects concentrated solution, obtain Limax mucus extract product 118g, yield 11.8%.
Embodiment 9
Get 4.5kg Limax mucus, be placed in and extract still, add the water of 22.5L, be cooled to 5 ℃, after being uniformly mixed, with extracting in high pressure homogenizer 1 time.Resulting extraction material centrifuge centrifugalize, disgorging matter.Centrifugal liquid is that 0.1 μ m microfiltration of ceramic membrane is processed with aperture, the daltonian ultrafilter membrane ultrafiltration of molecular cut off 6,000 for microfiltration dialysis solution, ultrafiltration dialysis liquid is concentrated by molecular cut off 80 dalton's NF membrane, collect concentrated solution, obtain Limax mucus extract product 841g, yield 18.7%.
Embodiment 10
Get 2.2kg Limax mucus, be placed in and extract still, add the water of 33.0L, be heated to 40 ℃, after being uniformly mixed, with extracting in high pressure homogenizer 2 times.Resulting extraction material centrifuge centrifugalize, disgorging matter.Centrifugal liquid is that 0.3 μ m microfiltration of ceramic membrane is processed with aperture, the daltonian ultrafilter membrane ultrafiltration of molecular cut off 10,000 for microfiltration dialysis solution, ultrafiltration dialysis liquid is concentrated by molecular cut off 500 dalton's NF membrane, collect concentrated solution, obtain Limax mucus extract product 702g, yield 31.9%.
Embodiment 11
Get 3.5kg Limax mucus, be placed in and extract still, add the water of 28.0L, after being uniformly mixed, with extracting in high pressure homogenizer 3 times under 25 ℃ of conditions of room temperature.Resulting extraction material centrifuge centrifugalize, disgorging matter.Centrifugal liquid is that 0.2 μ m microfiltration of ceramic membrane is processed with aperture, the daltonian ultrafilter membrane ultrafiltration of molecular cut off 6,000 for microfiltration dialysis solution, ultrafiltration dialysis liquid is concentrated by molecular cut off 1,000 dalton's NF membrane, collects concentrated solution, obtain Limax mucus extract product 2.1kg, yield 60.0%.
Embodiment 12
Get 3.0kg Limax mucus, be placed in and extract still, add the water of 30.0L, after being uniformly mixed, with extracting in high pressure homogenizer 2 times under 25 ℃ of conditions of room temperature.Resulting extraction material centrifuge centrifugalize, disgorging matter.Centrifugal liquid is that 0.3 μ m microfiltration of ceramic membrane is processed with aperture, the daltonian ultrafilter membrane ultrafiltration of molecular cut off 8,000 for microfiltration dialysis solution, ultrafiltration dialysis liquid is concentrated by molecular cut off 800 dalton's NF membrane, collect concentrated solution, obtain Limax mucus extract product 1.6kg, yield 53.3%.
Chondroitin sulfate, allantoin, the collagen content of the Limax mucus extract of above-described embodiment gained, and the molecular weight distribution result of boy's peptide gathers and sees the following form:
According to the regulation of < < cosmetics management and safety and Efficiency assessment > >, carried out the detection of skin irritation test, Ocular irritation experiment and the bacterial micro-organism aspect of Limax mucus extract, indices result all meets the requirement of cosmetics.
Limax mucus extract of the present invention can effectively improve Skin Cell differentiation of self and replication capacity, and former albumen has maintenance moisture, strengthens the effect of skin elasticity; Chondroitin sulfate is conducive to remove old cutin, reduces wrinkle of skin and cicatrix; Boy's polypeptide has stimulates skin regeneration, desalination mottle, promote the effect of skin quality and glossiness.Specifically detect referring to shown in Fig. 2-4.
The application of Limax mucus extract in the cosmetics such as cream frost, water preparation, emulsion, its preferred addition is 1-10%.Most preferred, the addition of Limax mucus extract is 5%.
Embodiment 13
An anti-apolexis composition, it comprises following parts by weight component:
Embodiment 14
An anti-apolexis composition, it comprises following parts by weight component:
Embodiment 15
An anti-apolexis composition, it comprises following parts by weight component:
The preparation method of above-mentioned a kind of anti-apolexis composition is as follows:
(1) first disodiumedetate is added in water and stirred, be incubated half an hour after being heated to 80-90 degree Celsius simultaneously;
(2) in (1) step water, add successively polyglycereol, hydrogenated starch hydrolysate, carrageenan and hyaluronate sodium, be stirred to completely after dissolving and start cooling;
(3) after temperature drops to 45-55 degree Celsius, add Aloe extract, trehalose, vitamin E and dextran, stir;
(4) add after cooling the temperature to 35-45 degree Celsius Limax mucus extract and, stir;
(5) finally add that with sodium hydroxide, to regulate pH value be 7-8, stir.
Embodiment 16
An anti-ageing face mask, it comprises following parts by weight component:
Embodiment 17
An anti-ageing face mask, it comprises following parts by weight component:
Embodiment 18
An anti-ageing face mask, it comprises following parts by weight component:
The preparation method of above-mentioned a kind of anti-ageing face mask is as follows:
(1) in water, add disodiumedetate (EDTA-2Na) to stir, be incubated half an hour after being heated to 80-90 degree Celsius simultaneously;
(2) add successively polyglycereol, hydrogenated starch hydrolysate, carrageenan and hyaluronate sodium, with the rotating speed of 20-50rpm, be stirred to after dissolving completely and start cooling;
(3) after temperature drops to 45-55 degree Celsius, add antiseptic, Aloe extract, trehalose, vitamin E and dextran, stir;
(4) after temperature is down to 35-45 degree Celsius, add Limax mucus extract, with the rotating speed of 20-50rpm, stir;
(5) finally add that with sodium hydroxide, to regulate pH value be 7-8, the rotating speed of 10-20rpm stirs.
The cosmetics that above-described embodiment is made do not have ill effect to skin, can effectively improve skin condition, make its activity recovery and elasticity, there are two moisturizing effects, can effectively improve Skin Cell differentiation of self and replication capacity, former albumen has maintenance moisture, strengthens the effect of skin elasticity; Chondroitin sulfate is conducive to remove old cutin, reduces wrinkle of skin and cicatrix; Boy's polypeptide has stimulates skin regeneration, desalination mottle, promote the effect of skin quality and glossiness.Specifically detect referring to shown in Fig. 2-4, it is consistent with said extracted quality testing survey effect that it detects effect.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (7)
1. a Limax mucus extract, is characterized in that, wherein contains chondroitin sulfate 0.1～5%, allantoin 0.1～5%, and collagen protein 0.1～10%, the molecular weight distribution of micromolecule polypeptide is 3000～5500 dalton.
2. a kind of Limax mucus extract according to claim 1, is characterized in that, described Limax mucus extract makes by following steps: after getting Limax mucus and mixing homogeneously with deionized water, with extracting in high pressure homogenizer; Extract material after centrifuge separation, obtain limpid extracting solution; Extracting solution is processed and is made bacteria-removing liquid through microfiltration; Bacteria-removing liquid ultra-filtration and separation; And then concentrated with nanofiltration.
3. the preparation method of a kind of Limax mucus extract according to claim 1, is characterized in that, it comprises the following steps:
(1) extract: get Limax mucus, add the water of 5～30 times of its quality, under 5～60 ℃ of temperature and 40～100Mpa pressure, carry out high-pressure homogenization historrhexis and extract 1～3 time;
(2) centrifugal: the high-pressure homogenization material of (1) step gained is separated with centrifuge, collect clear liquid;
(3) microfiltration: clear liquid is the microfiltration of ceramic membrane of 0.1 μ m-0.5 μ m with aperture;
(4) ultrafiltration: microfiltration dialysis solution molecular cut off is 6,000～50,000 daltonian ultrafilter membrane ultrafiltration;
(5) nanofiltration: ultrafiltration dialysis liquid is concentrated by molecular cut off 80～2,000 daltonian NF membrane, collects concentrated solution.
4. according to the preparation method of a kind of Limax mucus extract described in claims 3, it is characterized in that the mucus that the preferred white jade snail of described Limax mucus produces.
5. the application of Limax mucus extract in cosmetics, addition is 0.5-20%.
6. an anti-apolexis composition, is characterized in that, it comprises following parts by weight component:
7. an anti-ageing face mask, is characterized in that, it comprises following parts by weight component:
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Effective date of registration: 20190116 Address after: 510670 Unit 201-202, Second Floor, Area B, Guangzhou Innovation Base, 80 Luyue Road, Guangzhou High-tech Industrial Development Zone, Guangdong Province Patentee after: Luo Jie bio tech ltd, Guangzhou Address before: 510663 Unit 502-509 on the 5th floor of Area A and 602-611 on the 6th floor of Area C, 80 Lanyue Road, Guangzhou Science City, Guangdong Province Patentee before: Guangdong Baohua Test Center Co., Ltd. |