CN115998667A - Preparation method of microbial cytoplasmic macromolecule extract and cosmetic composition - Google Patents
Preparation method of microbial cytoplasmic macromolecule extract and cosmetic composition Download PDFInfo
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Abstract
The invention discloses a preparation method of a microbial cytoplasm macromolecular extract and a cosmetic composition, which take microorganisms as raw materials, destroy microbial cell walls by adopting a low-temperature wall breaking method, release soluble substances in microbial cytoplasm, then separate and remove solid substances in wall breaking mixed liquor to obtain soluble substances in microbial cytoplasm, then remove medium and small molecular substances containing color, smell and inorganic salt in the soluble substances by microfiltration to obtain concentrated macromolecular substances, and further remove the medium and small molecular substances by repeatedly diluting with water and carrying out microfiltration concentration to obtain a pure colorless, odorless and salt-free cytoplasmic macromolecular extract which keeps the bioactivity in the cytoplasmic macromolecular substances.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and in particular relates to a preparation method of a microbial cytoplasm macromolecule extract and a cosmetic composition.
Background
Cytoplasm is a generic term for all translucent, gelatinous, particulate matter surrounded by the cytoplasmic membrane except for the nuclear region. The water content is about 80%. The major components of the cytoplasm are ribosomes, reservoirs, various enzymes and intermediary metabolites, various nutrients and monomers of macromolecules, etc., and the cytoplasm includes stroma, organelles and inclusions.
The cytoplasmic matrix (cytoplasmic matrix), also known as cytosol, is a homogeneous and translucent colloidal fraction of the cytoplasm, filled between other tangible structures, accounting for about 55% of the total volume of the cell, in which thousands of enzymes are present, most of the intermediary metabolism including glycolysis, gluconeogenesis and synthesis of sugars, fatty acids, nucleotides and amino acids is carried out in the cytoplasmic matrix. About 20% of the cytoplasmic matrix is protein, and the chemical composition of the cytoplasmic matrix can be divided into three classes, small, medium and large, according to their relative molecular mass sizes. The small molecules comprise water, inorganic ions, small molecule organic matter monomers and the like; the medium molecules include lipids, oligosaccharides, oligopeptides, nucleotide derivatives, etc.; macromolecules include proteins, fats, lipoproteins, polysaccharides, RNA, and the like.
Organelles are structures distributed in the cytoplasm, have a morphology, and play an important role in physiological activities of cells. It comprises the following steps: mitochondria, endoplasmic reticulum, intranet apparatus, golgi apparatus, lysosomes, microfilaments, microtubules, centrosomes, ribosomes, etc., and the chemical components mainly comprise water, proteins and lipids, and in addition, contain small molecules such as coenzymes and nucleic acids in small amounts.
The inclusion is a structure in the cytoplasm that is not itself metabolically active, but has a specific morphology. Some are stored energy substances, such as glycogen particles, lipid droplets; some are cellular products such as secretory granules, melanin granules; the residue may also be considered as inclusion.
From the above description of the substances contained in the cytoplasm of the microorganism, it can be seen that the soluble substances in the cytoplasm mainly include proteins, lipids, saccharides, nucleic acids and inorganic substances.
Proteins include macromolecular proteins, small molecular proteins, polypeptides, oligopeptides and amino acids.
Lipids include large and small molecule fats and lipids, glycerol, fatty acids, and the like.
The saccharide includes polysaccharide, oligosaccharide, monosaccharide, etc.
From the above analysis of the soluble substances in cytoplasm, it is known that the cytoplasm contains macromolecular substances, middle molecular substances and small molecular substances, which are different in composition and different in effect, and if they are made to exert their effects sufficiently in application, particularly in the cosmetic field, they are separated so that they exert their effects truly, and that the cytoplasm contains color and odor substances and inorganic salts, which affect the quality of cosmetics, and therefore must be removed when applied to the cosmetic field.
The cytoplasm macromolecular substances mainly comprise bioactive soluble macromolecular substances such as protein, fat, lipoid, lipoprotein, polysaccharide, RNA and the like, have great benefits on human health, have multiple functions on protecting and repairing human skin so as to treat the human skin, provide bioactive macromolecular nutrient substances for skin cells, such as enzymes and the like required by a large amount of growth, promote the skin cells to be in an optimal growth state, ensure vigorous metabolism of the skin cells, and keep the skin in a healthy state, and have the main functions of moisturizing, resisting oxidation, inhibiting melanin generation, whitening, improving the skin state, improving skin roughness and the like in cosmetics, so that the microbial cytoplasm macromolecular extract plays an important role in the application of cosmetics.
During the fermentation culture process, the cytoplasm of the microorganism can contain yellow or yellowish-brown substances, substances with special odor and a large amount of inorganic salts, and if the traditional wall breaking technology such as autolysis (autolysis) and enzymolysis (enzymes hydrolysis) is adopted, the biological activity of the cytoplasm macromolecular substances of the microorganism can be destroyed.
The color causes pigmentation of skin and the smell causes unpleasant feeling of the produced cosmetics, so the color and smell seriously affect the quality of the produced cosmetics, the skin is stimulated by long-term contact with inorganic salt, especially the salt concentration is too high, corrosion is generated, skin allergy is caused, erythema, pimple, blister, seepage, itching and many other symptoms occur, and once the biological activity of the microbial cytoplasm macromolecular substances is destroyed in the production process, the substances cannot exert the due effect, reduce the use effect and further affect the quality of the cosmetics.
Therefore, if the cytoplasmic macromolecule extract is intended to be applied to the cosmetic field and exert its maximum efficacy, it is necessary to remove color and odor substances and inorganic salts from the cytoplasmic macromolecule extract and to maintain the biological activity of the substances in the cytoplasmic macromolecule extract.
In the existing cosmetic composition, only the application of the EXTRACT of the cytoplasmic mixture of microorganisms is included, wherein the EXTRACT contains both cytoplasmic macromolecular substances and middle molecular substances and small molecular substances containing color and smell substances and inorganic salts, such as YEAST EXTRACT (YEAST EXTRACT) and YEAST lysate EXTRACT (SACCHAROMYCES LYSATE EXTRACT), or the application of the EXTRACT of the microbial fermentation product and the cytoplasmic mixture, such as the lysate of the fermentation product of the two YEASTs (BIFIDA FERMENT LYSATE), because the EXTRACT of the two YEASTs is the mixture EXTRACT, the EXTRACT contains color and smell substances and inorganic salts which are harmful to the skin, the addition ratio of the EXTRACT containing the color and smell substances and the inorganic salts which are harmful to the skin in the application of the cosmetic is limited, the cosmetic with the EXTRACT as a main component cannot be produced, and the wall breaking method adopted by the EXTRACT can damage the biological activity of the substances in the cytoplasm, so that the substances in the cytoplasm cannot exert the efficacy of the substances.
Therefore, a set of preparation methods of microbial cytoplasmic macromolecular extracts capable of decoloring, deodorizing, desalting and maintaining the biological activity of the substances in cytoplasm are found, and a cosmetic composition which uses the microbial cytoplasmic macromolecular extracts as main components and uses cosmetic raw materials derived from microorganisms and/or plants as main other components is found to play a vital role in the application of the microbial cytoplasmic macromolecular extracts, particularly in the application of the microbial cytoplasmic macromolecular extracts as cosmetic raw materials.
Disclosure of Invention
The invention aims to provide a preparation method of a microbial cytoplasmic macromolecule extract which can decolor, deodorize and desalt and maintain the biological activity of the substances while maintaining the macromolecule substances in cytoplasm, and provides a novel cosmetic composition which is formed by taking the microbial cytoplasmic macromolecule extract as a main component and taking cosmetic raw materials derived from microorganisms and/or plants as main other components.
The invention aims at realizing the following technical scheme:
the preparation method of the microbial cytoplasmic macromolecule extract is characterized by comprising the following steps:
(1) Adding water into the used microbial raw materials, uniformly mixing to obtain microbial raw material diluent, and then separating the obtained microbial raw material diluent by using a separator to remove a liquid part to obtain microbial solids;
(2) Repeating the step (1) to obtain a clean microbial raw material;
(3) Adding water into the clean microorganism raw materials, and uniformly mixing to obtain uniform clean microorganism mixed solution;
(4) Breaking the wall of the clean microorganism mixed solution to obtain a wall-broken solution of the microorganism mixed solution;
(5) Separating the wall-broken liquid of the microorganism mixed liquid by using a separator, and separating and removing solid parts to obtain liquid containing microorganism cytoplasmic substances;
(6) Micro-filtering the obtained liquid containing the microbial cytoplasm substances by utilizing a micro-filtration membrane, and removing the medium and small molecular substances in the liquid to obtain concentrated microbial cytoplasm macromolecular substances;
(7) Adding water into the concentrated microbial cytoplasm macromolecular substance to obtain a microbial cytoplasm macromolecular substance diluent;
(8) Separating and concentrating the microbial cytoplasmic macromolecular substance diluent again by utilizing a microfiltration membrane to obtain a primary clean microbial cytoplasmic macromolecular substance concentrate;
(9) Repeating the steps (7) and (8) to remove medium and small molecular substances in the concentrated microbial cytoplasmic macromolecular substances to obtain pure microbial cytoplasmic macromolecular substance concentrate;
(10) Separating the obtained pure microorganism cytoplasmic macromolecular substance concentrate by using a separator to remove solid substances therein, and finally obtaining the microorganism cytoplasmic macromolecular extract.
The microbial feedstock in step (1) includes single-cell eukaryotic microorganisms and/or single-cell prokaryotic microorganisms.
The separators in the steps (1), (5) and (10) can separate solid and liquid phases in separated liquid, and the separators comprise a horizontal decanter centrifuge, a disc separator, a plate-and-frame filter press and the like.
The wall breaking process in the step (4) is a process performed at a low temperature, such as wall breaking using a homogenizer, or the like.
The medium and small molecular substances in the step (6) contain all color and smell substances and inorganic salts.
In the steps (1) to (10), enzymes, chemicals and the like which destroy the biological activity of substances in the cytoplasmic macromolecule extract of microorganisms are not added to the whole process, and the temperature is maintained at not more than 55 ℃ in the whole process.
The aperture of the microfiltration membrane in the step (6) is 0.05-1 μm.
A cosmetic composition comprising a microbial cytoplasmic macromolecule extract as a major component, characterized in that:
(1) The microbial cytoplasmic macromolecule extract is a product prepared according to the steps (1) to (10) in claim 1;
(2) The microbial cytoplasmic macromolecule extract can be used as a final product in the form of concentrated solution, or can be freeze-dried to be used as a final product in the form of freeze-dried powder;
(3) The content of medium molecular substances in the microbial cytoplasmic macromolecule extract is less than 0.1%;
(4) The content of small molecular substances in the microbial cytoplasmic macromolecule extract is less than 100ppm;
(5) The ratio of the microbial cytoplasmic macromolecule extract in the cosmetic composition is not less than 30%;
(6) The cosmetic composition includes other components.
The proportion of not less than 30% in the feature (5) is calculated on the basis of the weight of the cytoplasmic macromolecular extract of the microorganism, excluding water, based on the total weight of the cosmetic composition.
The ratio of the microorganism-derived and/or plant-derived cosmetic raw material in the other components described in the feature (6) is more than 90%, and the microorganism-derived and/or plant-derived cosmetic raw material means a cosmetic raw material extracted and purified from the microorganism, and the plant-derived cosmetic raw material means a cosmetic raw material extracted and purified from the plant, based on the weight of the microorganism-derived and/or plant-derived cosmetic raw material as a percentage of the total weight of the other components excluding water.
The invention has the advantages and beneficial effects that:
1. the product (microbial cytoplasmic macromolecule extract) produced by the present invention is a completely new product, and no existing patent or literature is presented for the same product as the product produced by the present invention.
2. The microbial cytoplasmic macromolecular extracts comprise bioactive soluble macromolecular substances such as protein, fat, lipoprotein, polysaccharide, RNA and the like, have great benefits on human health, have multiple functions on the protection and repair of human skin, and have multiple functions on treatment, and the soluble substances in the cytoplasmic macromolecules provide bioactive macromolecular nutrients for skin cells, such as enzymes and the like required by the growth of the skin cells, promote the skin cells to be in an optimal growth state, ensure the vigorous metabolism of the skin cells, and keep the skin in a healthy state.
3. The wall breaking process in the step (4) is a wall breaking process which can be performed at a low temperature, such as wall breaking by using a homogenizer, etc., and the low temperature wall breaking adopted by the invention does not damage the biological activity of substances in the cytoplasmic macromolecule extract, thereby ensuring the biological activity of the substances in the produced cytoplasmic macromolecule extract, such as the activity of enzymes, and only ensuring the activity of the substances to fully play the function of the substances.
4. The enzyme which damages the biological activity of the substances in the microbial cytoplasmic macromolecule extract is not added in the whole process, a large amount of substances with biological activity, such as enzyme and the like, are contained in the microbial cytoplasmic macromolecule extract, the biological activity of the substances in the microbial cytoplasmic macromolecule extract can be guaranteed only by maintaining the biological activity of the substances, and the substances can be damaged by adding the decomposition enzyme and chemical substances, such as strong acid, strong alkali and the like at high temperature during the use, so that the biological activity of the substances in the microbial cytoplasmic macromolecule extract is guaranteed.
5. The aperture of the microfiltration membrane in the step (6) is 0.05-1 mu m, so that the flexibility of the produced microbial cytoplasmic macromolecule extract product is ensured, the size of the aperture of the microfiltration membrane determines the molecular weight of the entrapped microbial cytoplasmic macromolecule extract, the smaller the aperture is, the smaller the molecular weight of the produced microbial cytoplasmic macromolecule extract is, and the microbial cytoplasmic macromolecule extract with different molecular weights is produced by adjusting the aperture size of the microfiltration membrane according to the requirement.
6. The invention thoroughly removes the medium and small molecular substances in the cytoplasm by the methods of repeated concentration, dilution and re-concentration in the steps (6), (7), (8) and (9), wherein the medium and small molecular substances in the cytoplasm comprise all color substances, smell substances and inorganic salts.
7. The invention removes the solid matters generated in the production process in the produced microbial cytoplasmic macromolecule extract through the final high separation factor separation, thereby ensuring that the produced microbial cytoplasmic macromolecule extract is completely dissolved in water.
8. In the existing microbial extracts, the dry matter content of the products is low, and the adding proportion of the microbial extracts in cosmetics is limited, and in the characteristic (2) of the cosmetic composition taking the microbial cytoplasmic macromolecular extracts as main components, the microbial cytoplasmic macromolecular extracts are defined to be in the form of concentrated solution or can be freeze-dried to be in the form of freeze-dried powder, and can be added according to any proportion as required due to the high dry matter content.
9. In the existing microbial extracts, there are microbial cytoplasm medium-molecular and small-molecular substances containing color substances, odor substances and inorganic salts which affect the quality of cosmetics, the addition ratio of them in cosmetics is limited, and the contents of medium-molecular and small-molecular substances in the microbial cytoplasm large-molecular extracts as main components are clearly defined in the features (3) and (4) of the present invention, and since the contents of medium-molecular and small-molecular substances therein are sufficiently low, they can be added in any ratio as required.
10. In the existing cosmetic composition, the weight proportion of the raw materials of the cosmetics derived from microorganisms and/or plants is low, so that the cosmetics derived from microorganisms and/or plants cannot be produced truly, and the main component derived from microorganisms used in the invention is a pure, colorless, odorless and salt-free extract of the cytoplasmic macromolecules of microorganisms which maintain the original biological activity, so that the raw materials can be added in any proportion according to the requirement, and other components in the cosmetic composition are defined in the characteristic (6), so that the invention is a real cosmetic composition derived from microorganisms and/or plants.
Drawings
In order that the present invention may be more clearly understood, the following description will be made in detail with reference to the accompanying drawings, wherein fig. 1 is a flow chart of a preparation process according to the present invention.
Detailed Description
The invention will be further described by means of specific examples.
Example 1
(1) Taking 800g of wet yeast (dry matter content is 33%) which is just cultured, adding 2400g of water, setting a separator to 4500 rpm, at which time the separation factor is 3622, separating for 10 minutes, and removing the supernatant to obtain yeast solid at the bottom;
(2) 2400g of water is added into the yeast solid, the mixture is stirred uniformly, the separator is set to 4500 r/min, the separation factor is 3622 at this time, the separation is carried out for 10 min, and the supernatant is removed, thus obtaining the yeast solid at the bottom;
(3) Repeating the step (2) for 4 times to obtain 784g (dry matter content is 32%) of clean yeast raw material;
(4) Adding 800g of water into a clean yeast raw material, uniformly stirring, then breaking the wall of the clean yeast raw material by using a homogenizer, setting the pressure of the homogenizer to 135MPa, setting the flow to 48ml/min, and obtaining a yeast wall breaking liquid after the circulation times are 5 times;
(5) Separating the yeast wall-broken liquid, wherein the rotating speed of the separator is set at 5000 r/min, the separating factor is 4472, the separation is carried out for 10 min, and the solid part is removed to obtain liquid containing yeast cytoplasmic substances;
(6) Micro-filtering the obtained liquid containing the yeast cytoplasmic substances by using a micro-filtration membrane, wherein the aperture of the micro-filtration membrane is 0.2 mu m, and removing medium and small molecular substances in the liquid by micro-filtration to obtain 316g (dry matter content is 20%) of concentrated yeast cytoplasmic macromolecular substances;
(7) Adding 948g of purified water into the concentrated yeast cytoplasmic macromolecular substance to obtain yeast cytoplasmic macromolecular substance diluent;
(8) Separating and concentrating the yeast cytoplasmic macromolecular substance diluent again by utilizing a microfiltration membrane to obtain 300g (dry matter content is 20%) of the primary clean yeast cytoplasmic macromolecular substance concentrate;
(9) Repeating the steps (7) and (8) for 4 times, thoroughly removing medium and small molecular substances in the concentrated solution of the yeast cytoplasmic macromolecular substances to obtain 284g (dry matter content is 20%) of pure concentrated solution of the yeast cytoplasmic macromolecular substances;
(10) Separating the obtained pure concentrated solution of yeast cytoplasmic macromolecular substances, wherein the rotation speed of the separator is set at 6000 rpm, the separation factor is 6439, and solid substances in the concentrated solution are removed, thereby obtaining 276g (dry matter content is 19.5%) of pure, colorless and odorless yeast cytoplasmic macromolecular extract with original bioactivity.
Detection of yeast cytoplasmic macromolecule extract:
measurement of chromaticity: the color tristimulus value and the chromatic aberration delta E of the general test method for cosmetics are adopted by the light industry standard QB/T2789-2006 of the people's republic of China * Measurement of (3), measurement results: average reading ΔE of two sample preparation measurements * 3.2, pure white.
Determination of smell: the sensory test shows that no special smell is generated during the fermentation culture process of the yeast.
Determination of inorganic salts: the detection result is 0.016S/m by adopting a conductivity meter (Lei Ci desk type conductivity meter DDS-307A), and the salt content in the yeast cytoplasmic macromolecule extract is less than 80ppm.
Example 2
(1) 20g of the yeast cytoplasmic macromolecule extract obtained in example 1 (dry matter content: 19.5%) was taken and placed in a graduated 100ml beaker.
(2) Taking 100mg of superoxide dismutase (SOD) with the biological activity of 7200U/mg, wherein the adopted superoxide dismutase is intracellular enzyme biosynthesized in vivo by utilizing yeast, and then obtaining blue-green crystals through wall breaking, filtering, purifying and freeze drying, the superoxide dismutase (SOD) is copper-containing and zinc-containing superoxide dismutase, and adding the 100mg of superoxide dismutase (SOD) into the beaker in the step (1).
(3) 1.5g of hyaluronic acid is taken and added into the beaker in the step (1), and the hyaluronic acid is obtained through microbial synthesis, extraction and purification.
(4) Adding 1.5g of glucan into the beaker in the step (1), wherein the glucan is processed by using yeast cell walls as raw materials.
(5) Taking 1.5g of mannan, adding the mannan into a beaker in the step (1), and processing the mannan by using yeast cell walls as raw materials.
(6) Adding ultrapure water into the beaker in the step (1), adding the beaker to the position with the scale of 100ml, and uniformly stirring to obtain the cosmetic which takes the yeast cytoplasmic macromolecule extract as a main component and takes superoxide dismutase (SOD), hyaluronic acid, glucan and mannans as other components and all components are derived from microorganisms.
Example 3
(1) 25g of the yeast cytoplasmic macromolecule extract obtained in example 1 (dry matter content 19.5%) was taken and placed in a graduated 100ml beaker.
(2) Taking 150mg of superoxide dismutase (SOD) with the biological activity of 7200U/mg, wherein the adopted superoxide dismutase is intracellular enzyme biosynthesized in vivo by utilizing yeast, and then obtaining blue-green crystals through wall breaking, filtering, purifying and freeze drying, the superoxide dismutase (SOD) is copper-containing and zinc-containing superoxide dismutase, and adding the 150mg of superoxide dismutase (SOD) into the beaker in the step (1).
(3) 1.2g of hyaluronic acid is taken and added into the beaker in the step (1), and the hyaluronic acid is obtained through microbial synthesis, extraction and purification.
(4) Adding 1.2g of glucan into the beaker in the step (1), wherein the glucan is processed by using yeast cell walls as raw materials.
(5) Taking 1.2g of mannan, adding the mannan into a beaker in the step (1), and processing the mannan by using yeast cell walls as raw materials.
(6) Taking 1.5g of maltodextrin, and adding the maltodextrin into a beaker in the step (1), wherein the maltodextrin is obtained by processing corn starch serving as a raw material.
(7) 1.5g of sesame oil is taken and added into the beaker in the step (1), and the sesame oil is obtained by processing sesame serving as a raw material.
(8) Adding ultrapure water into the beaker in the step (1), adding the beaker to the position with the scale of 100ml, and uniformly stirring to obtain the cosmetic which takes yeast cytoplasmic macromolecule extract as a main component and takes microorganism-derived cosmetic raw materials such as superoxide dismutase (SOD), hyaluronic acid, glucan and mannans, and plant-derived cosmetic raw materials such as maltodextrin and sesame oil as other components.
Example 4
(1) 28g of the yeast cytoplasmic macromolecule extract obtained in example 1 (dry matter content: 19.5%) was taken and placed in a graduated 100ml beaker.
(2) Taking 200mg of superoxide dismutase (SOD) with the biological activity of 7200U/mg, wherein the adopted superoxide dismutase is intracellular enzyme biosynthesized in vivo by utilizing yeast, and then obtaining blue-green crystals through wall breaking, filtering, purifying and freeze drying, and the superoxide dismutase (SOD) is copper-containing and zinc-containing superoxide dismutase, and adding the 200mg of superoxide dismutase (SOD) into the beaker in the step (1).
(3) Adding 1g of hyaluronic acid into the beaker in the step (1), wherein the adopted hyaluronic acid is obtained through microbial synthesis, extraction and purification.
(4) Adding 1g of glucan into the beaker in the step (1), wherein the glucan is processed by using yeast cell walls as raw materials.
(5) Taking 1g of mannan, adding the mannan into a beaker in the step (1), and processing the mannan by using yeast cell walls as raw materials.
(6) Taking 1.2g of maltodextrin, and adding the maltodextrin into a beaker in the step (1), wherein the maltodextrin is obtained by processing corn starch serving as a raw material.
(7) 1.2g of sesame oil is taken and added into the beaker in the step (1), and the sesame oil is obtained by processing sesame serving as a raw material.
(8) Adding 0.02g of methylparaben into the beaker in the step (1), wherein the methylparaben is obtained by using petroleum as a raw material and performing chemical processing.
(9) Adding 0.01g of essence into the beaker in the step (1), wherein the essence is obtained by using petroleum as a raw material and performing chemical processing.
(10) Adding ultrapure water into the beaker in the step (1), adding the beaker to the position with the scale of 100ml, and uniformly stirring to obtain cosmetics which take yeast cytoplasmic macromolecule extract as a main component, and take plant-derived cosmetic raw materials such as superoxide dismutase (SOD), hyaluronic acid, glucan and manna, and cosmetic raw materials such as maltodextrin and sesame oil and chemical-derived cosmetic raw materials such as hydroxybenzoyl ester and essence as other components, wherein the weight of the chemical-derived cosmetic raw materials accounts for 0.53 percent of the total weight of the other components and is less than 10 percent defined in the cosmetic composition.
Claims (11)
1. The preparation method of the microbial cytoplasmic macromolecule extract is characterized by comprising the following steps:
(1) Adding water into the used microbial raw materials, uniformly mixing to obtain microbial raw material diluent, and then separating the obtained microbial raw material diluent by using a separator to remove a liquid part to obtain microbial solids;
(2) Repeating the step (1) to obtain a clean microbial raw material;
(3) Adding water into the clean microorganism raw materials, and uniformly mixing to obtain uniform clean microorganism mixed solution;
(4) Breaking the wall of the clean microorganism mixed solution to obtain a wall-broken solution of the microorganism mixed solution;
(5) Separating the wall-broken liquid of the microorganism mixed liquid by using a separator, and separating and removing solid parts to obtain liquid containing microorganism cytoplasmic substances;
(6) Micro-filtering the obtained liquid containing the microbial cytoplasm substances by utilizing a micro-filtration membrane, and removing the medium and small molecular substances in the liquid to obtain concentrated microbial cytoplasm macromolecular substances;
(7) Adding water into the concentrated microbial cytoplasm macromolecular substance to obtain a microbial cytoplasm macromolecular substance diluent;
(8) Separating and concentrating the microbial cytoplasmic macromolecular substance diluent again by utilizing a microfiltration membrane to obtain a primary clean microbial cytoplasmic macromolecular substance concentrate;
(9) Repeating the steps (7) and (8) to remove medium and small molecular substances in the concentrated microbial cytoplasmic macromolecular substances to obtain pure microbial cytoplasmic macromolecular substance concentrate;
(10) Separating the obtained pure microorganism cytoplasmic macromolecular substance concentrate by using a separator to remove solid substances therein, and finally obtaining the microorganism cytoplasmic macromolecular extract.
2. The microorganism of step (1) of claim 1, wherein the microbial feedstock comprises single-cell eukaryotic microorganisms and/or single-cell prokaryotic microorganisms.
3. The separator according to claim 1, wherein the separator is capable of separating solid and liquid phases in the separated liquid, and comprises a decanter centrifuge, a disc separator, a plate and frame filter press, etc.
4. The wall breaking process according to claim 1, wherein the wall breaking process is a process performed at a low temperature, such as wall breaking with a homogenizer, or the like.
5. The medium and small molecular substances according to claim 1, wherein the medium and small molecular substances comprise all color and odor substances and inorganic salts.
6. The process according to claim 1, wherein the whole process is free from enzymes, chemicals, etc. which disrupt the biological activity of the substances in the cytoplasmic macromolecular extracts of microorganisms, and the temperature is maintained at not more than 55 ℃ in the whole process.
7. The microfiltration according to claim 1, wherein the microfiltration membrane has a pore size of 0.05-1 μm.
8. A cosmetic composition comprising a microbial cytoplasmic macromolecule extract as a major component, characterized in that:
(1) The microbial cytoplasmic macromolecule extract is a product prepared according to the steps (1) to (10) in claim 1;
(2) The microbial cytoplasmic macromolecule extract can be used as a final product in the form of concentrated solution, or can be freeze-dried to be used as a final product in the form of freeze-dried powder;
(3) The content of medium molecular substances in the microbial cytoplasmic macromolecule extract is less than 0.1%;
(4) The content of small molecular substances in the microbial cytoplasmic macromolecule extract is less than 100ppm;
(5) The ratio of the microbial cytoplasmic macromolecule extract in the cosmetic composition is not less than 30%;
(6) The cosmetic composition includes other components.
9. The composition according to claim 8, wherein the proportion of the extract of the cytoplasmic macromolecules of the microorganisms is not less than 30%, based on the weight of the extract of the cytoplasmic macromolecules of the microorganisms, which are calculated as a percentage of the total weight of the cosmetic composition, excluding water.
10. The other component according to claim 8, wherein the proportion of the cosmetic raw material derived from the microorganism and/or the plant in the other component is more than 90%, based on the weight of the cosmetic raw material derived from the microorganism and/or the plant in the total weight of the other component, excluding water.
11. The cosmetic raw material derived from a microorganism and/or a plant according to claim 10, wherein the cosmetic raw material derived from a microorganism is a cosmetic raw material extracted and purified from a microorganism, and the cosmetic raw material derived from a plant is a cosmetic raw material extracted and purified from a plant.
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| CN112545928A (en) * | 2019-09-25 | 2021-03-26 | 华南协同创新研究院 | Tremella compound extract and preparation method and application thereof |
| CN115074393A (en) * | 2022-08-04 | 2022-09-20 | 江苏创健医疗科技有限公司 | Pichia pastoris fermentation lysate filtrate, preparation method and application |
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| CN116042402A (en) * | 2023-02-09 | 2023-05-02 | 北京活力蓝晶生物科技有限公司 | Production process of microbial cytoplasmic macromolecule extract |
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