CN105777933A - Preparation of algal polysaccharide and application of algal polysaccharide in cosmetics - Google Patents
Preparation of algal polysaccharide and application of algal polysaccharide in cosmetics Download PDFInfo
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- CN105777933A CN105777933A CN201610118886.9A CN201610118886A CN105777933A CN 105777933 A CN105777933 A CN 105777933A CN 201610118886 A CN201610118886 A CN 201610118886A CN 105777933 A CN105777933 A CN 105777933A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/001—Preparations for care of the lips
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0084—Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates
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Abstract
The invention provides preparation of algal polysaccharide and application of algal polysaccharide in cosmetics.The preparation method of the algal polysaccharide includes the steps that algae are smashed and then purified with methanol; after methanol in residues is sufficiently removed, acid-soluble polysaccharide in the residues is extracted with a hot acid extraction method; macromolecular polysaccharide ingredients with low bioavailability are removed through ethanol precipitation; after ethanol in the residual solution is recycled, dialysis and freeze-drying are carried out, and the algal polysaccharide is obtained.The molecular weight of the algal polysaccharide is 8-35 kDa, and the algal polysaccharide has significant antioxidation and moisturizing activity and can be applied to the field of cosmetics.Moisturizer prepared from the algal polysaccharide is used on the skin surfaces of the hands, the face, the neck and the like and has the effects of nourishing and moisturizing the skin, reducing wrinkles and delaying ageing.Eye cream prepared from the algal polysaccharide is used around the eyes and has the effects of nourishing and moisturizing the skin, reducing wrinkles and delaying ageing.
Description
Technical field
The present invention relates to cosmetic field, especially relate to the preparation of a kind of Sargassum polysaccharides and the application in cosmetics thereof.
Background technology
Sargassum is submarine algae, is botanic cryptogam, and algae includes that several inhomogeneities are with photosynthesis
Produce the biology of energy.They are generally considered to be simple plant, are mainly characterized by: without vascular tissue, the trueest
Positive root, stem, the differentiating phenomenon of leaf；Do not bloom, without fruit and seed；Genitals is without the protective tissue of specialization, Chang Zhi
Connect and produced spore or gamete by single cell；And the formation without embryo.The general designation of marine products algae (Algae), generally
Admittedly write on seabed or certain solid structure, it is the individual plant that constituted of basal cell or a lot of simple plant.With Sargassum
The biology run after fame enumerates a variety of, and these body differences are huge, across multiple life entity, common ground mainly life
In the seawater, synthesis of organic substance can be carried out by self internal chromatoplast and photosynthesis.
Sargassum fusiforme (Harv.) Setch, a kind of algae, for the one in Sargassum, call Cornu Cervi point, Ottelia acuminata (Gagnep.) Dandy bud, sheep milk Fructus Hordei Vulgaris sub, extra large
Deng.Frond yellowish-brown, plump succulence, thallophytic variation is very big, and shape is various.It is grown on low tide band rock,
In state of China North gets Liaodong Peninsula, to the Lezhou Peninsula, all there is its distribution in south；Most with Along Zhejiang Coast.
Sargassum is used as medicine in the existing history remote of China, Shennong's Herbal written by Southern Qi Dynasty's TAO Hong-Jing (Christian era 452～536)
On describe Sargassum and describe dietetic therapy character and Application way.Also have blood fat reducing, antithrombotic and elimination brain tired simultaneously
Labor, promotion childhood development, enhancement effect such as immunity of organisms, slow down aging.
Sargassum fusiforme (Harv.) Setch is Sargassaceae sea-plant, and its dry frond is included by Sargassum, containing Multiple components in Chinese Pharmacopoeia
Such as polysaccharide, protein, polyphenol, mineral and various trace element etc., have radioprotective, antitumor, immunomodulating,
The effects such as antioxidation.The application in cosmetics of its extract the most gradually increases, and has moisturizing, sun-proof, regulation skin
Fat secretion, slow down aging etc. act on.And Hijiki polysaccharide has antitumor action, there is promotion hemopoietic function and strengthen immunity
Effect of function, can prevent thrombosis, reduces cholesterol and prevents hypertension.
In prior art, the extraction process of Hijiki polysaccharide is focused mainly on the moisturizing activity of extract, patent
CN101250232A discloses the extraction process of a kind of Sargassum fusiforme (Harv.) Setch active polysaccharide, carry in conjunction with water, alkali carries, hot water carries and
Ultrasonic technology extracts Sargassum fusiforme (Harv.) Setch active polysaccharide.But in the method, the stage of only extracting just have employed first normal-temperature water and carries, then ice
Bathe ultrasonic Treatment, then 70-80 DEG C of water carries, the most again by the method for potass extraction, there is the problem that operation is numerous and diverse.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide the preparation of a kind of Sargassum polysaccharides and in cosmetics
Application.The molecular weight of gained Sargassum polysaccharides is 8-35kDa, for acid-soluble polysaccharide, have notable antioxidation and
Moisturizing activity, it is adaptable to preparation moisturizing class and defying age class cosmetics.
It is an object of the invention to be achieved through the following technical solutions:
The invention provides the preparation method of a kind of Sargassum polysaccharides, described preparation method is: through first after being pulverized by Sargassum
Alcohol remove impurity, recycles hot acid extraction method and extracts the most acid-soluble polysaccharide, then pass through ethanol after the abundant methanol removal of residue
The sedimentation method remove the macromolecular polysaccharide constituents that bioavailability is relatively low, after surplus solution reclaims ethanol, and membrane filtration,
Lyophilization, obtains the Sargassum polysaccharides of molecular weight 8-35kDa.
Described Sargassum includes Sargassum fusiforme (Harv.) Setch.
Preferably, the specifically comprising the following steps that of described preparation method
Remove impurity: extracting drying the Sargassum pulverized 2-4 hour with methanol eddy, filter, filtrate reclaims methanol；Filter
Slag volatilizes at residual methanol, 45-50 DEG C the driest, standby；
Extract: being extracted by dried filtering residue hydrochloric acid solution, Extracting temperature is 70-75 DEG C, extract 2-4 little
Time, filter, filtrate is evaporated under vacuum, obtains semifluid shape crude extract；
Precipitate with ethanol: the crude extract that obtained by extraction step is soluble in water, adds ethanol, after stirring, quiet
Put 24-48 hour, filter, after filtrate removes ethanol, standby；
Dialysis: gained filtrate after precipitate with ethanol is used membrane filter method molecular cut off is the Sargassum polysaccharides of 8-35kDa, dense
Contracting, lyophilization, to obtain final product.
The optimum extraction temperature of the present invention is at 70-75 DEG C, if temperature is too low, extraction ratio is the highest；Temperature is too high, institute
Obtain polyoses extract performance of keeping humidity to reduce.
Preferably, in described removal step: Sargassum with the solid-to-liquid ratio of methanol is, every 1g Sargassum correspondence 20-30mL
Methanol, i.e. 1:20-1:30 (g/mL).
Preferably, in described extraction step, the molar concentration of hydrochloric acid solution is 0.1-0.2M；Filtering residue and the material of hydrochloric acid solution
Liquor ratio is, the hydrochloric acid solution of every 1g filtering residue correspondence 15-30mL, i.e. 1:15-1:30 (g/mL)；Vacuum condition is vacuum
Degree 0.085-0.098Mpa.Described concentration of hydrochloric acid is too low, and antioxygenic property can be caused to reduce；Excessive concentration can cause moisturizing
Performance reduces.In solid-liquid ratio, hydrochloric acid solution crosses that I haven't seen you for ages causes antioxygenic property to reduce, and hydrochloric acid solution too much increases follow-up dense
Shorten this into.
Preferably, in described precipitate with ethanol step, the ethanol of addition volumetric concentration in water is 30-50%.
Present invention also offers Sargassum polysaccharides that the preparation method of a kind of described Sargassum polysaccharides the obtains use in cosmetics
On the way.
Present invention also offers a kind of skin lotion containing Sargassum polysaccharides, in terms of mass fraction 100%, including following mass fraction
Each component: aforesaid Sargassum polysaccharides 1-3%, Aloe polysaccharide extracting solution 10-20%, silicone grease 5-10%, caprylic/capric glycerol
Three ester 3-6%, Jojoba oil 3-6%, octadecanol 2-5%, Brij72 polyoxyethylene stearyl base ether 2-4%, Brij721 polyoxy
Ethylene stearyl ether 2-4%, butanediol 3-5%, Acritamer 940 0.2-0.5%, phenoxyethanol 0.5-0.8%, ethylhexyl
Glycerol 0.5-0.8%, EDETATE SODIUM 0.05-0.1%, triethanolamine 0.2-0.4%, essence 0.3-0.8%, surplus for go from
Sub-water.
Present invention also offers the preparation method of a kind of skin lotion containing Sargassum polysaccharides, comprise the following steps:
S1, weigh each component raw material:
Oil phase: silicone grease, caprylic/capric triglyceride, Jojoba oil, octadecanol, Brij72 polyoxyethylene stearyl base ether 2,
Brij721 polyoxyethylene stearyl base ether；
Aqueous phase: Sargassum polysaccharides, Aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
After add phase: essence, phenoxyethanol, Sensiva SC50, triethanolamine；
Prepared by S2, oil phase mixed liquor: mixed by each for oil phase raw material, and heated and stirred is uniform, obtains oil phase mixed liquor；
Prepared by S3, aqueous phase mixed liquor: first swell in deionized water by Acritamer 940, is configured to solution A, then by solution
A mixes with remaining Aqueous Phase Raw Material, heating mixing, obtains aqueous phase mixed liquor；
S4, three-phase mix: joined by aqueous phase mixed liquor in oil phase mixed liquor, homogenizing, after stirring cooling, after adding
Each component raw material in adding mutually, then homogenizing, evacuation, be stirred for cooling, to obtain final product.
Preferably, in described step S2, it is heated to 85 ± 2 DEG C；
In described step S3, the mass concentration of solution A is 1%, is heated to 60-70 DEG C；
Described step S4 particularly as follows: aqueous phase mixed liquor is slowly added in oil phase mixed liquor, 3000rpm homogenizing 20min,
Stirring froth breaking, is cooled to 45-50 DEG C, is kept stirring for 30min, each component raw material in adding mutually after adding, 2000rpm
Homogenizing 15min, evacuation de-bubbled, stirring cooling, to obtain final product.
Present invention also offers a kind of eye cream containing Sargassum polysaccharides, in terms of mass fraction 100%, including following mass fraction
Each component: aforesaid Sargassum polysaccharides 1-5%, Aloe polysaccharide extracting solution 10-20%, Flos Camelliae Japonicae caul-fat 3-5%, shea butter 3-5%,
Butanediol 5-10%, squalane 5-10%, Cera Flava 3-6%, cetearyl alcohol and cetearyl glucoside Montanov68
3-6%, Acritamer 940 0.3-0.5%, sodium hydroxide 0.1-0.5%, pantothenylol 0.5-2%, Vitamin E acetate 0.5-2%,
Ceramide 0.1-0.2%, phenoxyethanol 0.3-0.5%, Sensiva SC50 0.3-0.5%, hyaluronate sodium 0.1-0.3%,
Olibanum quintessence oil 0.3-1%, essence 0.2-0.5%, EDETATE SODIUM 0.05-0.1%, surplus is deionized water.
Present invention also offers the preparation method of a kind of skin lotion containing Sargassum polysaccharides, comprise the following steps:
A1, weigh each component raw material:
Oil phase: Flos Camelliae Japonicae caul-fat, shea butter, squalane, Cera Flava, cetearyl alcohol and cetearyl glucoside
Montanov68, ceramide, caprylic/capric triglyceride, Jojoba oil, octadecanol；
Aqueous phase: Sargassum polysaccharides, Aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
After add phase: Olibanum quintessence oil, Vitamin E acetate, pantothenylol, sodium hyaluronate, essence, phenoxyethanol, ethyl
Hexyl glycerol, sodium hydroxide；
Prepared by A2, oil phase mixed liquor: mixed by each for oil phase raw material, and heated and stirred is uniform, obtains oil phase mixed liquor；
Prepared by A3, aqueous phase mixed liquor: first swell in deionized water by Acritamer 940, is configured to solution B, then by solution
B mixes with remaining Aqueous Phase Raw Material, heating mixing, obtains aqueous phase mixed liquor；
A4, three-phase mix: be slowly added in oil phase mixed liquor by aqueous phase mixed liquor, homogenizing, stirring cooling, add
After add mutually in each component raw material, then homogenizing, evacuation, stirring cooling, to obtain final product.Weigh each component raw material:
Preferably, in described step A2, it is heated to 80-85 DEG C；
In described step A3, the mass concentration of solution B is 1%, is heated to 70-75 DEG C；
Described step A4 particularly as follows: aqueous phase mixed liquor is slowly added in oil phase mixed liquor, 3000rpm homogenizing 20min,
Stirring froth breaking, is cooled to 50-55 DEG C, is kept stirring for 30min, each component raw material in adding mutually after adding, 2000rpm
Homogenizing 15min, evacuation de-bubbled, stirring cooling, to obtain final product.
Compared with prior art, the present invention has a following beneficial effect:
The Sargassum polysaccharides molecular weight of gained of the present invention is relatively low, compared with high molecular Sargassum polysaccharides, is retaining close moisture retention
While there is higher non-oxidizability, trophism and penetration, can accelerate the metabolism of cell, increase skin elasticity,
Delaying skin aging.And there is good water solublity, can in cosmetics with other raw material interworkings, have widely should
Use prospect.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in those skilled in the art
Member is further appreciated by the present invention, but limits the present invention the most in any form.It should be pointed out that, the common skill to this area
For art personnel, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement.These broadly fall into
Protection scope of the present invention.
Sargassum employed in the following embodiment of the present invention and comparative example is Sargassum fusiforme (Harv.) Setch.In the Sargassum polysaccharides detection of preparation,
The mensuration of described hydroscopicity and moisturizing rate is carried out according to following method.
Hydroscopicity assay method: precision weighs a certain amount of testing sample and (is denoted as W0), put into the cultivation of a diameter of 7cm
In ware, culture dish is positioned in exsiccator, replaces silica gel with saturated ammonium sulfate solution bottom exsiccator, keep relatively
Humidity is 81%, and temperature is 25 DEG C, weight W of test sample product after placement a period of timen, hydroscopicity %=(Wn-W0)/W0*100。
Moisturizing rate assay method: testing sample is made into the solution that mass fraction is 1%, precision pipettes 10mL in a diameter of
In the culture dish of 7cm, being positioned in exsiccator by culture dish, keeping relative humidity is 81%, and temperature is 25 DEG C.Respectively
Weigh and place water content H after a period of timenWith water content H before placement0, moisturizing rate %=100*Hn/H0。
Embodiment 1
Present embodiments provide the preparation method of a kind of Sargassum polysaccharides, comprise the following steps:
Step one: remove impurity
It is (1:20) by solid-to-liquid ratio g/mL, extracts drying the Sargassum pulverized 4 hours with methanol eddy, filter, filter
Liquid reclaims methanol；Filtering residue volatilizes residual methanol, the driest at 45 DEG C, standby.
Step 2: extract
By dried filtering residue, extracting with the hydrochloric acid solution of 0.2M, filtering residue is 1:30 with the solid-liquid ratio of hydrochloric acid solution,
Extracting temperature is 75 DEG C, extracts 2 hours, filters, and filtrate is steamed under vacuum is 0.098Mpa near dry, obtains half
Flow-like crude extract.
Step 3: precipitate with ethanol
Ethanol is slowly added in above-mentioned crude extract, and is stirred continuously, be 50% to concentration of alcohol, stand 48 hours, mistake
Filter, filtrate recycling ethanol, standby.
Step 4: dialysis
It is the polysaccharide of 8-35kDa by step 3 gained sample solution employing membrane filter method molecular cut off, concentrates, freezing dry
Dry, obtain the Sargassum polysaccharides that molecular weight is 8-35kDa.
In the research carrying out extraction process, the present embodiment 1 is with the non-oxidizability of extract and moisture retention as guide, right
Being raised each position and carry out the mensuration of non-oxidizability and performance of keeping humidity respectively, result is shown in Tables 1 and 2 respectively.
Table 1 non-oxidizability measurement result
Table 2 moisture absorption and moisture retention measurement result
As can be known from the results of Table 1, gained Sargassum polysaccharides of the present invention has more significant antioxidant activity.Result from table 2 can
Knowing, gained Sargassum polysaccharides of the present invention has good antioxidant activity simultaneously, it may have significantly moisturizing activity.
Embodiment 2
Present embodiments provide the preparation method of a kind of Sargassum polysaccharides, comprise the following steps:
Step one: remove impurity
It is (1:30) by solid-to-liquid ratio g/mL, extracts drying the Sargassum pulverized 2 hours with methanol eddy, filter, filter
Liquid reclaims methanol；Filtering residue volatilizes residual methanol, the driest at 45 DEG C, standby.
Step 2: extract
By dried filtering residue, extracting with the hydrochloric acid solution of 0.2M, filtering residue is 1:15 with the solid-liquid ratio of hydrochloric acid solution,
Extracting temperature is 70 DEG C, extracts 4 hours, filters, and filtrate is steamed under vacuum is 0.085Mpa near dry, obtains half
Flow-like crude extract.
Step 3: precipitate with ethanol
Ethanol is slowly added in above-mentioned crude extract, and is stirred continuously, be 30% to concentration of alcohol, stand 36 hours, mistake
Filter, filtrate recycling ethanol, standby.
Step 4: dialysis
It is the polysaccharide of 8-35kDa by step 3 gained sample solution employing membrane filter method molecular cut off, concentrates, freezing dry
Dry, obtain the Sargassum polysaccharides that molecular weight is 8-35kDa.
Embodiment 3
Present embodiments provide the preparation method of a kind of Sargassum polysaccharides, comprise the following steps:
Step one: remove impurity
It is (1:25) by solid-to-liquid ratio g/mL, extracts drying the Sargassum pulverized 3 hours with methanol eddy, filter, filter
Liquid reclaims methanol；Filtering residue volatilizes residual methanol, the driest at 45 DEG C, standby.
Step 2: extract
By dried filtering residue, extracting with the hydrochloric acid solution of 0.1M, filtering residue is 1:20 with the solid-liquid ratio of hydrochloric acid solution,
Extracting temperature is 70 DEG C, extracts 4 hours, filters, and filtrate is steamed under vacuum is 0.085Mpa near dry, obtains half
Flow-like crude extract.
Step 3: precipitate with ethanol
Ethanol is slowly added in above-mentioned crude extract, and is stirred continuously, be 40% to concentration of alcohol, stand 24 hours, mistake
Filter, filtrate recycling ethanol, standby.
Step 4: dialysis
It is the polysaccharide of 8-35kDa by step 3 gained sample solution employing membrane filter method molecular cut off, concentrates, freezing dry
Dry, obtain the Sargassum polysaccharides that molecular weight is 8-35kDa.
Comparative example 1
This comparative example is identical with the preparation method of embodiment 1, the difference is that only: in step 2, and this comparative example uses
Extraction with aqueous solution.
Comparative example 2
This comparative example is identical with the preparation method of embodiment 1, is only that: in step 2 before difference, and this comparative example uses
Extracting temperature be 80 DEG C.
Comparative example 3
This comparative example is identical with the preparation method of embodiment 1, is only that: in step 2 before difference, and this comparative example uses
The solid-liquid ratio of filtering residue and hydrochloric acid solution be 1:10.
Embodiment 2 and 3, comparative example 1-3 extraction process in, raised each position and carried out non-oxidizability and moisture retention respectively
Can mensuration, the results are shown in Table 3 and table 4.
Table 3 embodiment 2 and 3, the non-oxidizability measurement result of comparative example 1-3
The moisture absorption of the Sargassum polysaccharides that table 4 embodiment 2 and 3, comparative example 1-3 obtain and moisture retention measurement result
Knowable to the result of table 1 and table 3, the embodiment of the present invention is compared with comparative example, and gained Sargassum polysaccharides has the most anti-
Oxidation activity.The result of table 2 and table 4 understands, and the embodiment of the present invention, compared with comparative example, is possessing good oxidation resistance
There is more preferable performance of keeping humidity simultaneously.
The embodiment 2 skin lotion containing Sargassum polysaccharides
Present embodiments providing a kind of skin lotion containing Sargassum polysaccharides, its preparation method is as follows:
Oil phase: silicone grease (9040), caprylic/capric triglyceride, Jojoba oil, octadecanol, Brij72 polyoxyethylene
Stearyl ether 2, Brij721 polyoxyethylene stearyl base ether.
Aqueous phase: the Sargassum polysaccharides of molecular weight 8-35kDa, Aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDTA bis-
Sodium, deionized water.
After add phase: essence, phenoxyethanol, Sensiva SC50, triethanolamine.
The parts by weight of above-mentioned each raw material components are as follows:
The Sargassum polysaccharides 1-3% of molecular weight 8-35kDa, Aloe polysaccharide extracting solution 10-20%, silicone grease (9040) 5-10%, pungent
Acid/tricaprin 3-6%, Jojoba oil 3-6%, octadecanol 2-5%, Brij72 polyoxyethylene stearyl base ether 2-4%,
Brij721 polyoxyethylene stearyl base ether 2-4%, butanediol 3-5%, Acritamer 940 0.2-0.5%, phenoxyethanol 0.5-0.8%,
Sensiva SC50 0.5-0.8%, EDETATE SODIUM 0.05-0.1%, triethanolamine 0.2-0.4%, essence 0.3-0.8%, remaining
Amount is deionized water.
Step: each for oil phase raw material is added and becomes owner of emulsifying pot heated and stirred to 85 ± 2 DEG C, treat fully to dissolve mixing；Aqueous phase is former
Acritamer 940 is first fully swollen in deionized water by material, is configured to the solution of 1%, then adds with remaining Aqueous Phase Raw Material
Auxiliary emulsifying pot, mixing, it is heated to 60-70 DEG C.Aqueous phase is slowly added to oil phase, and 3000rpm homogenizing 20min, stirring disappears
Bubble, is cooled to 45-50 DEG C, is kept stirring for 30min, adds phase after addition, 2000rpm homogenizing 15min, evacuation de-bubbled,
Stirring cooling, to obtain final product.
Emollient cream of present invention preparation, for skin surfaces such as hand, face, cervical regions, has moist moisturizing, alleviates wrinkle
Stricture of vagina, effect of slow down aging.
The embodiment 3 eye cream containing Sargassum polysaccharides
Present embodiments providing a kind of eye cream containing Sargassum polysaccharides, its preparation method is as follows:
Oil phase: silicone grease (9040), caprylic/capric triglyceride, Jojoba oil, octadecanol, Brij72 polyoxyethylene
Stearyl ether 2, Brij721 polyoxyethylene stearyl base ether；
Aqueous phase: the Sargassum polysaccharides of molecular weight 8-35kDa, Aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDTA bis-
Sodium, deionized water；
After add phase: essence, phenoxyethanol, Sensiva SC50, triethanolamine；
The parts by weight of above-mentioned each raw material components are as follows:
The Sargassum polysaccharides 1-5% of molecular weight 8-35kDa, Aloe polysaccharide extracting solution 10-20%, Flos Camelliae Japonicae caul-fat 3-5%, breast wood
Fruit oil 3-5%, butanediol 5-10%, squalane 5-10%, Cera Flava 3-6%, cetearyl alcohol (with) cetearyl Portugal
Glucosides Montanov683-6%, Acritamer 940 0.3-0.5%, sodium hydroxide 0.1-0.5%, pantothenylol 0.5-2%, dimension
Raw element E acetate 0.5-2%, ceramide 0.1-0.2%, phenoxyethanol 0.3-0.5%, Sensiva SC50 0.3-0.5%,
Hyaluronate sodium 0.1-0.3%, Olibanum quintessence oil 0.3-1%, essence 0.2-0.5%, EDETATE SODIUM 0.05-0.1%, surplus
For deionized water.
Step: each for oil phase raw material is added and becomes owner of emulsifying pot heated and stirred to 80-85 DEG C, treat fully to dissolve mixing；Aqueous phase is former
Acritamer 940 is first fully swollen in deionized water by material, is configured to the solution of 1%, then adds with remaining Aqueous Phase Raw Material
Auxiliary emulsifying pot, mixing, it is heated to 70-75 DEG C.Aqueous phase is slowly added to oil phase, and 3000rpm homogenizing 20min, stirring disappears
Bubble, is cooled to 50-55 DEG C, is kept stirring for 30min, adds phase after addition, 2000rpm homogenizing 15min, evacuation de-bubbled,
Stirring cooling, to obtain final product.
The eye cream of present invention preparation, for circumference of eyes, has moist moisturizing, alleviates effect of wrinkle, slow down aging.
Additionally, with the effectiveness comparison of embodiment in patent CN101250232A for, Sargassum prepared by the inventive method is many
Sugar non-oxidizability is higher, and the performance of keeping humidity that invention also provides extract investigates data, shows that moistening effect is notable.
In sum, the present invention prepares the Sargassum polysaccharides that gained molecular weight is 8-35kDa, has notable antioxidation
With moisturizing activity, it is adaptable to preparation moisturizing class and defying age class cosmetics.And there is good water solublity, can make up
With other raw material interworkings in product, it is with a wide range of applications.
The above-mentioned description to embodiment is to be understood that for ease of those skilled in the art and use invention.Ripe
Know the personnel of art technology and obviously can easily these embodiments be made various amendment, and described herein typically
Principle is applied in other embodiments without through performing creative labour.Therefore, the invention is not restricted to above-described embodiment,
Those skilled in the art should be at this according to the announcement of the present invention, the improvement made without departing from scope and amendment
Within the protection domain of invention.
Claims (10)
1. the preparation method of a Sargassum polysaccharides, it is characterised in that described preparation method comprises the steps: sea
Through methanol remove impurity after algae pulverizing, recycle hot acid extraction method after the abundant methanol removal of residue and extract the most acid-soluble many
Sugar, then remove, by precipitate with ethanol, the macromolecular polysaccharide constituents that bioavailability is relatively low, after surplus solution reclaims ethanol,
Dialysis, lyophilization, obtain the Sargassum polysaccharides of molecular weight 8-35kDa.
The preparation method of Sargassum polysaccharides the most according to claim 1, it is characterised in that described preparation method
Specifically comprise the following steps that
Remove impurity: extracting drying the Sargassum pulverized 2-4 hour with methanol eddy, filter, filtrate reclaims methanol；Filter
Slag volatilizes at residual methanol, 45-50 DEG C the driest, standby；
Extract: being extracted by dried filtering residue hydrochloric acid solution, Extracting temperature is 70-75 DEG C, extract 2-4 little
Time, filter, filtrate is evaporated under vacuum, obtains crude extract；
Precipitate with ethanol: the crude extract that obtained by extraction step is soluble in water, adds ethanol, after stirring, quiet
Put 24-48 hour, filter, after filtrate removes ethanol, standby；
Dialysis: gained filtrate after precipitate with ethanol is used membrane filter method molecular cut off is the Sargassum polysaccharides of 8-35kDa, dense
Contracting, lyophilization, to obtain final product.
The preparation method of Sargassum polysaccharides the most according to claim 2, it is characterised in that in described removal step:
Sargassum with the amount ratio of methanol is, the methanol of every 1g Sargassum correspondence 20-30mL.
The preparation method of Sargassum polysaccharides the most according to claim 2, it is characterised in that in described extraction step,
The molar concentration of hydrochloric acid solution is 0.1-0.2M；Filtering residue with the solid-liquid ratio of hydrochloric acid solution is, every 1g filtering residue is corresponding
The hydrochloric acid solution of 15-30mL；Vacuum condition is vacuum 0.085-0.098Mpa.
The preparation method of Sargassum polysaccharides the most according to claim 2, it is characterised in that in described precipitate with ethanol step,
The ethanol added volumetric concentration in water is 30-50%.
6. the Sargassum polysaccharides obtained according to the preparation method of Sargassum polysaccharides described in any one of Claims 1 to 5 is being changed
Purposes in cosmetic.
7. the skin lotion containing Sargassum polysaccharides, it is characterised in that in terms of mass fraction 100%, divide including following quality
Each component of number: Sargassum polysaccharides 1-3% prepared by method described in any one of Claims 1 to 5, Aloe polysaccharide extracting solution
10-20%, silicone grease 5-10%, caprylic/capric triglyceride 3-6%, Jojoba oil 3-6%, octadecanol 2-5%, Brij72
Polyoxyethylene stearyl base ether 2-4%, Brij721 polyoxyethylene stearyl base ether 2-4%, butanediol 3-5%, Acritamer 940
0.2-0.5%, phenoxyethanol 0.5-0.8%, Sensiva SC50 0.5-0.8%, EDETATE SODIUM 0.05-0.1%, three ethanol
Amine 0.2-0.4%, essence 0.3-0.8%, surplus is deionized water.
8. the preparation method of the skin lotion containing Sargassum polysaccharides according to claim 7, it is characterised in that include
Following steps:
S1, weigh each component raw material:
Oil phase: silicone grease, caprylic/capric triglyceride, Jojoba oil, octadecanol, Brij72 polyoxyethylene stearyl base ether 2,
Brij721 polyoxyethylene stearyl base ether；
Aqueous phase: Sargassum polysaccharides, Aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
After add phase: essence, phenoxyethanol, Sensiva SC50, triethanolamine；
Prepared by S2, oil phase mixed liquor: mixed by each for oil phase raw material, and heated and stirred is uniform, obtains oil phase mixed liquor；
Prepared by S3, aqueous phase mixed liquor: first swell in deionized water by Acritamer 940, is configured to solution A, then by solution
A mixes with remaining Aqueous Phase Raw Material, heating mixing, obtains aqueous phase mixed liquor；
S4, three-phase mix: joined by aqueous phase mixed liquor in oil phase mixed liquor, homogenizing, after stirring cooling, after adding
Each component raw material in adding mutually, then homogenizing, evacuation, stirring cooling, to obtain final product.
9. the eye cream containing Sargassum polysaccharides, it is characterised in that in terms of mass fraction 100%, including following mass fraction
Each component: Sargassum polysaccharides 1-5% prepared by method described in any one of Claims 1 to 5, Aloe polysaccharide extracting solution 10-20%,
Flos Camelliae Japonicae caul-fat 3-5%, shea butter 3-5%, butanediol 5-10%, squalane 5-10%, Cera Flava 3-6%, cetearyl alcohol and
Cetearyl glucoside Montanov68 3-6%, Acritamer 940 0.3-0.5%, sodium hydroxide 0.1-0.5%, pantothenylol
0.5-2%, Vitamin E acetate 0.5-2%, ceramide 0.1-0.2%, phenoxyethanol 0.3-0.5%, ethylhexyl is sweet
Oil 0.3-0.5%, hyaluronate sodium 0.1-0.3%, Olibanum quintessence oil 0.3-1%, essence 0.2-0.5%, EDETATE SODIUM
0.05-0.1%, surplus is deionized water.
10. the preparation method of the eye cream containing Sargassum polysaccharides according to claim 9, it is characterised in that include
Following steps:
A1, weigh each component raw material:
Oil phase: Flos Camelliae Japonicae caul-fat, shea butter, squalane, Cera Flava, cetearyl alcohol and cetearyl glucoside
Montanov68, ceramide, caprylic/capric triglyceride, Jojoba oil, octadecanol；
Aqueous phase: Sargassum polysaccharides, Aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
After add phase: Olibanum quintessence oil, Vitamin E acetate, pantothenylol, sodium hyaluronate, essence, phenoxyethanol, ethyl
Hexyl glycerol, sodium hydroxide；
Prepared by A2, oil phase mixed liquor: mixed by each for oil phase raw material, and heated and stirred is uniform, obtains oil phase mixed liquor；
Prepared by A3, aqueous phase mixed liquor: first swell in deionized water by Acritamer 940, is configured to solution B, then by solution
B mixes with remaining Aqueous Phase Raw Material, heating mixing, obtains aqueous phase mixed liquor；
A4, three-phase mix: be slowly added in oil phase mixed liquor by aqueous phase mixed liquor, homogenizing, stirring cooling, add
After add mutually in each component raw material, then homogenizing, evacuation, stirring cooling, to obtain final product.
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