CN111544372A

CN111544372A - Fermentation product compound plant extract anti-aging repair composition, application and essence milk containing composition

Info

Publication number: CN111544372A
Application number: CN202010543687.9A
Authority: CN
Inventors: 万笑宇
Original assignee: Soyounglife Technology Co ltd
Current assignee: Soyounglife Technology Co ltd
Priority date: 2020-06-15
Filing date: 2020-06-15
Publication date: 2020-08-18

Abstract

The invention provides an anti-aging repair composition prepared by compounding a fermentation product with a plant extract, application thereof and essence milk containing the composition, wherein the anti-aging repair composition comprises the fermentation product extract and the plant extract in parts by weight, and the fermentation product extract comprises the following components: 20-120 parts of yeast/grape fermentation product extract, 45-180 parts of secondary fission yeast fermentation product filtrate/lysate/extract and 30-160 parts of alteromonas fermentation product extract; the plant extract comprises: 2-20 parts of perilla leaf extract, 3-16 parts of lantana leaf extract, 2-14 parts of bidens pilosa extract, 5-40 parts of aesculus hippocastanum extract, 5-20 parts of thyme extract, 3-36 parts of aloe barbadensis extract, 3-34 parts of centella asiatica extract and 2-12 parts of silybum marianum extract. The yeast extract composition is compounded with the plant extract composition with strong oxidation resistance, provides rich active nutrition for skin, repairs injuries, twists and senilisms, and has extremely strong wound healing efficacy.

Description

Fermentation product compound plant extract anti-aging repair composition, application and essence milk containing composition

Technical Field

The invention relates to the field of compositions, and in particular relates to a fermentation product compounded plant extract anti-aging and repairing composition, application thereof and essence milk containing the composition.

Background

With the increase of age, the skin begins to be in an aging state, mainly comprising the reduction of the content of collagen, elastin, mucopolysaccharide and the like in the skin in different degrees, the atrophy of blood vessels for supplying skin nutrition, the reduction of the elasticity of blood vessel walls, the gradual thinning and swelling of skin epidermis, the reduction of subcutaneous fat, and the visual manifestation of wrinkles, chloasma and senile plaques.

The belief that aging is a continuation of the activity developed by a genetically-set organism has been partially negated. Aging is now recognized as a balance between the body's repair potential and the frequency and intensity of the injuries it suffers. Skin is the most damaging organ to the environment, and ultraviolet radiation and free radicals produced by metabolism in the body are considered to be the most important harmful substances that accelerate the aging of cellular proteins, lipids, polysaccharides and DNA.

Specifically, the increase of one free radical (free radical) is an atom or molecule with unpaired electrons generated by the homolytic cleavage of covalent bonds, has high chemical activity, and is oxidized with unsaturated lipids to generate Lipid Peroxides (LPO), and the final product Malondialdehyde (MDA) can react with most substances of living cells to reduce the permeability of biological membranes, damage DNA molecules and cause cell death or mutation. Second, medium-wave Ultraviolet (UVB) and long-wave Ultraviolet (UVA) in sunlight cause skin photoaging. Whereas ultraviolet radiation causes skin aging mainly by the following mechanisms: firstly, DNA is damaged; collagen crosslinking; thirdly, the immune response is reduced by inducing the inhibition way of the antigen stimulation reaction; generating highly reactive free radicals to interact with various intracellular structures to cause the damage of cells and tissues; fifthly, directly inhibiting the function of epidermal Langerhans cells to cause photo-immune inhibition and weaken the immune function of skin. In addition, skin aging can be affected by nonenzymatic glycosylation, metabolic imbalance, matrix metalloproteinase aging, and the like.

Extensive research on these factors has led to the production of anti-aging skin care products. In order to achieve a good anti-aging effect, some anti-aging cosmetics use industrial ingredients with strong oxidation resistance, and the anti-aging cosmetics take effect quickly in a short time but are extremely harmful to human bodies after being used for a long time. The cosmetic prepared by using the plant extract as an active ingredient overcomes the defect that the traditional cosmetic depends on a chemical synthetic product, so that the safety performance of the product is higher; the natural components are easier to be absorbed by the skin, so that the effect of the product is more obvious; the functionality is more prominent. The plant extract as natural elastase inhibitor is the hot spot of recent research, such as scutellaria baicalensis, sanguisorba officinalis, morinda citrifolia seeds, moringa oleifera, poa aquatica, forsythia suspensa, salvia miltiorrhiza, angelica sinensis and the like.

However, the efficacy of a single plant extract is limited, and the search for more excellent plant extract combinations capable of being comprehensively conditioned in vast plants and the adoption of the composition which can be compounded with the plant extracts to form more powerful, comprehensive and excellent compositions are research hotspots which need to be broken through in the field.

Disclosure of Invention

In view of the above, in order to overcome the defects of the prior art, the invention provides an anti-aging and repairing composition of a fermentation product and a compound plant extract.

The anti-aging repair composition provided by the invention comprises a fermentation product extract and a plant extract, wherein the fermentation product extract comprises: 20-120 parts of yeast/grape fermentation product extract, 45-180 parts of secondary fission yeast fermentation product filtrate/lysate/extract and 30-160 parts of alteromonas fermentation product extract; the plant extract comprises: 2-23 parts of perilla leaf extract, 3-21 parts of lantana leaf extract, 2-18 parts of bidens pilosa extract, 5-40 parts of aesculus hippocastanum extract, 5-32 parts of thyme extract, 3-36 parts of aloe barbadensis extract, 3-34 parts of centella asiatica extract and 2-12 parts of silybum marianum extract.

Preferably, the anti-aging repair composition consists of 80 to 110 parts by weight of yeast/grape fermentation product extract, 140-165 parts by weight of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 100-120 parts by weight of alteromonas fermentation product extract, 13 to 18 parts by weight of perilla leaf extract, 6 to 10 parts by weight of lantana leaf extract, 11 to 14 parts by weight of bidens pilosa extract, 22 to 32 parts by weight of aesculus hippocastanum extract, 12 to 15 parts by weight of thyme extract, 26 to 30 parts by weight of aloe vera extract, 20 to 31 parts by weight of centella asiatica extract and 7 to 11 parts by weight of silybum marianum extract.

The invention also provides the application of the composition or the preferable composition in preparing skin care products.

The invention also provides the skin anti-aging repair essence milk which comprises 8-32% of the anti-aging repair composition or the optimal composition, 13-26% of essence milk base components and the balance of deionized water in percentage by mass.

Further, the essence milk also comprises regeneration and repair active polypeptide according to the mass percentage, wherein the regeneration and repair active polypeptide consists of 0.3-0.7% of acetyl hexapeptide-8, 0.1% -0.3% of palmitoyl pentapeptide-3 and 0.001-0.1% of pentapeptide-3.

Further, the essence milk also comprises antioxidant active ingredients according to the mass percentage, wherein the antioxidant active ingredients comprise 0.02-0.06% of kinetin, 2-4% of tea polyphenol, 0.5-1% of hydroxydecyl ubiquinone, 1-5% of colloidal oat powder and 0.5-1.5% of tocopherol.

Further, the essence milk also comprises active ingredients for promoting microcirculation and natural moisturizing active factors according to the mass percentage, wherein the active ingredients for promoting microcirculation comprise 0.1-0.3% of arginine, 0.1-1.5% of adenosine and 0.01-1% of biotin; the natural moisturizing active factor consists of 0.01-0.08% of sodium hyaluronate and 0.2-0.5% of wild mango kernel fat.

Further, the essence milk is added with 1% of natural whitening component tetrahydrocurcumin by mass percent.

Further, the essence milk base component comprises, by weight, 8 parts of glycerin, 1 part of butanediol, 1 part of propylene glycol, 1 part of caprylic/capric triglyceride, 1 part of polydimethylsiloxane, 1 part of hydrogenated coconut oil glyceride, 1 part of isononyl isononanoate, 0.2 part of stearyl alcohol, 1 part of cetyl alcohol, 1 part of jojoba esters, 0.2 part of glyceryl stearate, 0.2 part of hydrogenated lecithin, 3 parts of steareth-2, 1 part of steareth-21, 1 part of polymethyl methacrylate, 1 part of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, 0.2 part of xanthan gum, 1 part of hydroxyethyl cellulose, 0.1 part of titanium dioxide, 0.5 part of phenoxyethanol, 1 part of disodium EDTA, 1 part of chlorphenesin, 1,2 pentanediol, 1 part of ethylhexylglycerin, 1 part of p-hydroxyacetophenone, 1 part of potassium sorbate, 1 part of colorant 19140, 1 part of colorant CI14700 and 1 part of essence.

The invention also provides a preparation method of the skin anti-aging repair essence milk, which comprises the following steps:

1) adding water into a water phase pot, adding glycerol, butanediol, propylene glycol, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, xanthan gum, hydroxyethyl cellulose, titanium dioxide, phenoxyethanol, p-hydroxyacetophenone, EDTA disodium and 1,2 pentanediol according to the weight ratio under the condition of stirring, and then stirring and heating to 75-85 ℃ to obtain a water phase solution;

2) adding caprylic/capric triglyceride, polydimethylsiloxane, hydrogenated coconut oil glyceride, isononyl isononanoate, stearyl alcohol, cetyl alcohol, jojoba esters, glyceryl stearate, hydrogenated lecithin, steareth-2, steareth-21, polymethyl methacrylate, chlorphenesin and ethylhexylglycerin into an oil phase pot in proportion, and heating to 75-85 ℃ to melt the materials to obtain an oil phase solution;

3) vacuum pumping the water phase solution into a main pot of a homogenizing and liquefying machine, vacuum pumping the oil phase solution into the main pot of the homogenizing and liquefying machine under stirring, opening the homogenizing and liquefying machine, and homogenizing at the rotating speed of 2800r/min for 7-10 minutes; homogenizing, stirring at 40r/min, and keeping the temperature at 80 deg.C for 5 min;

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding the fermentation product compound plant extract anti-aging repair composition, regeneration repair active polypeptide, antioxidant active ingredient, natural moisture-keeping active factor, microcirculation promotion active ingredient and tetrahydrocurcumin, and fully and uniformly stirring;

5) adding potassium sorbate, a coloring agent CI19140, a coloring agent CI14700 and essence into the material obtained in the step 4); stirring uniformly, standing for 1 hour, continuing stirring for 5-10 minutes, and adjusting the pH value to 6.0-6.5; and (6) ending.

The extract according to the present invention means a solution obtained by immersing or mixing a substance to be extracted in a solvent. The extraction process can be carried out with the aid of the selection of suitable solvents, such as: water, ethanol/water mixture, methanol, propanol, butanol, chloroform, acetone or other organic solvent, and extracting by separation, percolation, decoction, countercurrent extraction, ultrasonic extraction, microwave extraction, or supercritical fluid extraction to obtain extract, and further evaporating and concentrating the extract to obtain extract and dried extract.

The fermentation product is compounded with the fermentation product extract in the plant extract anti-aging repair composition:

the yeast/grape fermentation product extract is from fermentation products of grape substrate culture, contains thalli and all active ingredients synthesized in the fermentation process, contains peptidoglycan, teichoic acid, protein, phospholipid, sterol, fatty acid, various enzymes, peptide, amino acid, nucleotide, exopolysaccharide and the like, provides rich nutrition for skin, and has good anti-aging effect.

The fermentation product filtrate/lysate/extract of the yeast bifidus is cultured, inactivated and decomposed by bifidobacterium to obtain metabolite, cytoplasm fragments, cell wall components and polysaccharide complex. It has no relation with yeast itself. The lysate of the yeast fermentation product has strong anti-immunosuppression activity, can promote DNA repair, can effectively protect skin from being damaged by ultraviolet rays, and helps to prevent photoaging of epidermis and dermis. Meanwhile, small molecules including vitamin B group, minerals, amino acids and the like beneficial to skin care can be generated, the metabolism of the skin cuticle can be enhanced, free radicals can be captured, lipid peroxidation can be inhibited, and the whitening and anti-aging functions are achieved. The product is rich in nutrients, and has skin nourishing effect.

The alteromonas fermentation product extract is extracted after fermentation of marine strains, can be dispersed on the horny layer of the skin surface to prevent skin dryness, has good moisturizing property, can increase skin elasticity by promoting collagen synthesis, can stimulate the growth of skin collagen fibroblasts, and can accelerate wound healing.

The compounded plant extract composition of the invention has a detoxification and anti-allergy combination and a repairing and conditioning combination.

Detoxification and anti-allergy combination: the perilla leaf relieves allergic skin; herba Bidentis Bipinnatae has effects of removing toxic substance, relieving swelling, reducing red blood streak, and soothing red skin; the lantana camara has the effects of clearing heat and removing toxicity, dispelling wind and relieving itching, and relieving the symptoms of allergic skin pruritus, and is especially suitable for acne muscles, allergic skin and hormone faces.

Repairing and conditioning combination:

the Aesculus hippocastanum extract can accelerate the self-repair ability of skin cells, improve microcirculation and maintain the normal metabolism of cells. The aescin can maintain the health of blood vessel, increase blood circulation, reduce capillary blood vessel penetration and reduce red blood streak; and edema of the eye due to poor circulation of blood vessels. Wherein the aescin can repair inflammation and promote wound healing: the effects of eliminating edema, removing acne and repairing are achieved through the activated leucocyte, and the anti-skin inflammation and the promotion of wound healing are achieved. The escin has the effects of resisting free radicals and oxidation, and can effectively resist aging and improve skin microcirculation after being frequently used.

The thyme extract inhibits cyclic AMP phosphodiesterase activity and promotes arginase activity, indicating anti-inflammatory and wound healing effects; can eliminate free radicals, can be used as antioxidant, and has antiaging effect; it also can be used as skin red blood streak preventing agent, antibacterial and dandruff removing agent, humectant and insect repellent.

The Aloe Barbadensis Miller extract contains polysaccharides, anthraquinone compounds, proteins, vitamins, and minerals. Can comprehensively repair the damaged tissues of the human body, quickly eliminate various toxins in the human body, enhance the immunity of the organism, delay the aging of the skin, decompose fat, reduce the weight, whiten the skin and improve simple obesity.

Centella asiatica extracts exhibit a broad spectrum of biological activities, most importantly antioxidant, anti-inflammatory, antibacterial and anticancer activities. In dermatology, centella asiatica extracts are used for the treatment of small wounds, value-added scars, burns, psoriasis, etc. Centella asiatica extract stimulates fibroblast proliferation and activates SMAD signaling pathways, thus increasing collagen type I production and reducing the formation of stretch marks and inflammatory responses.

Silymarin has effects of resisting radiation and aging, preventing and treating arteriosclerosis, and delaying skin aging.

Further compounded regeneration repair active polypeptide:

acetyl hexapeptide-8: is a bionic peptide and a novel hexapeptide, and has better effect particularly on lightening the depth of eye wrinkles by being covered with the reputation of 'wrinkle-reducing new star'. The composition can inhibit the release of nerve conduction element acetylcholine and weaken the contraction of muscles, so that dynamic line expression lines are reduced, wrinkles are reduced, the activity of elastin is increased, the renewal capacity of epidermis is maintained, the skin is smooth and compact, the skin age is challenged to be reversed, and the youthful luster of the skin is displayed.

Palmitoyl pentapeptide-3: the active small molecule in (1) is micro-collagen, and the small molecule polypeptide penetrates into the skin through cream containing palmitoyl pentapeptide-3 and reaches fibroblasts. Small molecules of the connective tissue of the skin, such as collagen and sucromosamine, are synthesized in fibroblasts and these synthesized molecules participate in the formation of the skin matrix.

Pentapeptide-3: is an improved peptide with lipophilicity, and can effectively relieve facial muscle contraction and increase the arrangement of collagen and elastin, thereby preventing skin wrinkles and maintaining skin tightness.

The invention has the beneficial effects that:

1. the fermentation product extract is compounded with the plant extract composition with strong oxidation resistance, so that rich active nutrition is provided for skin, damage is repaired, and premature senility is twisted. Has strong wound healing effect.

2. According to the invention, the regeneration and repair active polypeptide is compounded on the basis of compounding the plant extract with strong antioxidant activity and the yeast extract with rich nutrition, so that the skin is repaired, protected and nourished in an all-around manner, the skin is far away from the damage of free radicals and ultraviolet rays, the elasticity and tenderness are recovered, and the trace of the corrosion of the years is eliminated.

3. The novel cosmetic functional component combination for delaying skin aging provided by the invention overcomes the defects in the prior art. The invention starts from the mechanism of skin aging formation, regulates aging gene expression, prevents the damage of ultraviolet rays to the skin, eliminates harmful free radicals, enhances cell proliferation activity, reconstructs extracellular matrix, reduces the activity of MMPs protease, prevents the degradation of various extracellular matrixes such as collagen I, collagen III, collagen IV and the like, proteoglycan, elastin, laminin and the like, reduces the level of inflammatory factors induced by skin aging, supplements various amino acids required by collagen synthesis, promotes collagen synthesis, regulates the metabolism level of skin cells and stimulates the cell activity. In addition, the skin barrier function is enhanced on the outer layer of the skin, the capacity of skin cells for resisting external injury is improved, the water content of the skin is improved, the elasticity of the skin is recovered, and the skin aging is comprehensively delayed. Not only makes each component fully play its own role, but also realizes the synergistic effect.

The specific implementation mode is as follows:

the following examples are illustrative of the present invention and the present invention is not limited to the following examples.

Preparation of the anti-aging repair composition

Example 1:

weighing 80 parts of yeast/grape fermentation product extract, 180 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 110 parts of alteromonas fermentation product extract, 16 parts of perilla leaf extract, 14 parts of lantana leaf extract, 12 parts of bidens pilosa extract, 42 parts of aesculus hippocastanum extract, 18 parts of thyme extract, 29 parts of aloe vera extract, 26 parts of centella asiatica extract and 10 parts of silybum marianum extract by weight, and uniformly mixing to form a fermentation product compound plant extract composition.

Example 2:

weighing 100 parts of yeast/grape fermentation product extract, 150 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 115 parts of alteromonas fermentation product extract, 15 parts of perilla leaf extract, 8 parts of lantana leaf extract, 13 parts of bidens pilosa extract, 28 parts of aesculus hippocastanum extract, 14 parts of thyme extract, 28 parts of aloe vera extract, 28 parts of centella asiatica extract and 9 parts of silybum marianum extract by weight, and uniformly mixing to form a fermentation product compound plant extract composition.

Example 3:

weighing 120 parts of yeast/grape fermentation product extract, 120 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 50 parts of alteromonas fermentation product extract, 4 parts of perilla leaf extract, 12 parts of lantana leaf extract, 3 parts of bidens pilosa extract, 22 parts of aesculus hippocastanum extract, 7 parts of thyme extract, 10 parts of aloe vera extract, 9 parts of centella asiatica extract and 6 parts of silybum marianum extract by weight, and uniformly mixing to form a fermentation product compound plant extract composition.

Example 4:

weighing 80 parts of yeast/grape fermentation product extract, 140 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 100 parts of alteromonas fermentation product extract, 13 parts of perilla leaf extract, 6 parts of lantana leaf extract, 11 parts of bidens pilosa extract, 22 parts of aesculus hippocastanum extract, 12 parts of thyme extract, 26 parts of aloe vera extract, 20 parts of centella asiatica extract and 7 parts of silybum marianum extract by weight, and uniformly mixing to form a fermentation product compound plant extract composition.

Example 5:

weighing 110 parts of yeast/grape fermentation product extract, 165 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 120 parts of alteromonas fermentation product extract, 18 parts of perilla leaf extract, 10 parts of lantana leaf extract, 14 parts of bidens pilosa extract, 32 parts of aesculus hippocastanum extract, 15 parts of thyme extract, 30 parts of aloe vera extract, 31 parts of centella asiatica extract and 11 parts of silybum marianum extract by weight, and uniformly mixing to form a fermentation product compound plant extract composition.

Example 6:

weighing 20 parts of yeast/grape fermentation product extract, 45 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 30 parts of alteromonas fermentation product extract, 2 parts of perilla leaf extract, 3 parts of lantana leaf extract, 2 parts of bidens pilosa extract, 5 parts of aesculus hippocastanum extract, 5 parts of thyme extract, 3 parts of aloe vera extract, 3 parts of centella asiatica extract and 2 parts of silybum marianum extract by weight, and uniformly mixing to form a fermentation product compound plant extract composition.

Preparation of essence milk base component:

example 7:

weighing 80 parts of glycerol, 10 parts of butanediol, 10 parts of propylene glycol, 10 parts of acrylic acid (acrylate)/C10-30 alkanol acrylate cross-linked polymer, 2 parts of xanthan gum, 10 parts of hydroxyethyl cellulose, 1 part of titanium dioxide, 5 parts of phenoxyethanol, 10 parts of p-hydroxyacetophenone, 10 parts of EDTA disodium and 10 parts of 1,2 parts of pentanediol according to parts by weight, and uniformly mixing to form the essence milk substrate component 1.

Weighing 10 parts of caprylic/capric triglyceride, 10 parts of polydimethylsiloxane, 10 parts of hydrogenated cocoglyceride, 10 parts of isononyl isononanoate, 2 parts of stearyl alcohol, 10 parts of cetyl alcohol, 10 parts of jojoba esters, 2 parts of glyceryl stearate, 2 parts of hydrogenated lecithin, 30 parts of steareth-2, 10 parts of steareth-21, 10 parts of polymethyl methacrylate, 10 parts of chlorphenesin and 10 parts of ethylhexyl glycerol according to parts by weight, and uniformly mixing to form the essence milk base component 2.

Weighing 10 parts of potassium sorbate, 10 parts of a colorant CI19140, 10 parts of a colorant CI14700 and 10 parts of essence according to parts by weight, and uniformly mixing to form the essence milk base component 3.

Preparing the anti-aging repair essence milk:

example 8:

the anti-aging repair essence milk is prepared according to the following steps:

1) adding 1616g of water to a water phase kettle, and adding 162g of the essential milk base component 1 of example 7 with stirring; then stirring and heating to 75-85 ℃ to obtain an aqueous phase solution;

2) adding 138g of the essence emulsion base component 2 of the embodiment 7 into an oil phase pot, and heating to 75-85 ℃ to melt the materials to obtain an oil phase solution;

3) pumping the water phase solution into a main pot of a homogenizing and emulsifying machine in vacuum, pumping the oil phase solution into the main pot of the homogenizing and emulsifying machine in vacuum under stirring, opening the homogenizing and emulsifying machine, and homogenizing for 7-10 minutes at the rotating speed of 2800 r/min; homogenizing, stirring at 40r/min, and keeping the temperature at 80 deg.C for 5 min;

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 643g of the fermentation product compound plant extract composition obtained in the embodiment 1, and fully and uniformly stirring;

5) adding 41g of the essential milk base component 3 of example 7 to the material obtained in step 4); stirring uniformly, standing for 1 hour, continuing stirring for 5-10 minutes, and adjusting the pH value to 6.0-6.5; and (6) ending.

Example 9:

1) adding 905g of water to a water phase kettle, adding 319g of the essential milk base component 1 of example 7 under stirring; then stirring and heating to 75-85 ℃ to obtain an aqueous phase solution;

2) 275g of the essential milk base component 2 of example 7 was added to an oil phase pan and heated to 75-85 ℃ to melt the material and obtain an oil phase solution;

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 832g of the compound plant extract composition of the fermentation product of the example 2, 0.52g of kinetin, 77.1g of tea polyphenol, 18g of hydroxydecyl ubiquinone, 51.4g of colloidal oat powder, 15.6g of tocopherol and 26g of tetrahydrocurcumin, and fully and uniformly stirring;

5) adding 80g of the essence milk base component 3 of the example 7 into the material obtained in the step 4); stirring uniformly, standing for 1 hour, continuing stirring for 5-10 minutes, and adjusting the pH value to 6.0-6.5; and (6) ending.

Example 10:

1) adding 1242g of water to a water phase kettle, adding 246g of the essential milk base ingredient 1 of example 7 with stirring; then stirring and heating to 75-85 ℃ to obtain an aqueous phase solution;

2) adding 212g of the essence emulsion base component 2 in the embodiment 7 into an oil phase pot, and heating to 75-85 ℃ to melt the materials to obtain an oil phase solution;

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 520g of the fermentation product compound plant extract composition of the embodiment 3, 1.56g of kinetin, 78g of tea polyphenol, 26g of hydroxydecyl ubiquinone, 78g of colloidal oat flour, 23.4g of tocopherol, 5.2g of arginine, 39g of adenosine, 26g of biotin, 2g of sodium hyaluronate, 13g of wild mango nut butter and 26g of tetrahydrocurcumin, and fully and uniformly stirring;

5) 62g of the essential milk base component 3 of example 7 was added to the material obtained in step 4); stirring uniformly, standing for 1 hour, continuing stirring for 5-10 minutes, and adjusting the pH value to 6.0-6.5; and (6) ending.

Example 11:

1) 1564g of water was added to the water phase kettle and 184g of the essential milk base ingredient 1 of example 7 was added with stirring; then stirring and heating to 75-85 ℃ to obtain an aqueous phase solution;

2) 159g of the essence emulsion base component 2 in the embodiment 7 is added into an oil phase pot and heated to 75-85 ℃ to melt the materials, so as to obtain an oil phase solution;

4) after the heat preservation, cooling water is turned on, the mixed material obtained in the step 3) is cooled to 40-45 ℃, and 390g of the example is added

4, mixing the fermentation product of the composition with a plant extract composition, 18.2g of acetyl hexapeptide-8, 7.8g of palmitoyl pentapeptide-3, 2.6g of pentapeptide-3, 1.04g of kinetin, 52g of tea polyphenol, 23.4g of hydroxydecyl ubiquinone, 104g of colloidal oat flour, 18.2g of tocopherol, 5.2g of arginine, 39g of adenosine, 2g of biotin, 1.56g of sodium hyaluronate and 10.4 g of wild mango nut butter, and fully and uniformly stirring;

5) adding 47g of the essence milk base component 3 of the example 7 into the material obtained in the step 4); stirring uniformly, standing for 1 hour, continuing stirring for 5-10 minutes, and adjusting the pH value to 6.0-6.5; and (6) ending.

Example 12:

1) 1260g of water was added to the water phase kettle and 258g of the essential milk base ingredient 1 of example 7 was added with stirring; then stirring and heating to 75-85 ℃ to obtain an aqueous phase solution;

2) adding 223g of the essence emulsion base component 2 in the embodiment 7 into an oil phase pot, and heating to 75-85 ℃ to melt the materials to obtain an oil phase solution;

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 572g of the fermentation product compound plant extract composition obtained in the example 5, 13g of acetyl hexapeptide-8, 5.2g of palmitoyl pentapeptide-3, 0.026g of pentapeptide-3, 0.78g of kinetin, 78g of tea polyphenol, 20.8g of hydroxydecyl ubiquinone, 104g of colloidal oat flour and 18.2g of tocopherol, and fully and uniformly stirring;

5) adding 65.4g of the essence milk base component 3 of the example 7 into the material obtained in the step 4); stirring uniformly, standing for 1 hour, continuing stirring for 5-10 minutes, and adjusting the pH value to 6.0-6.5; and (6) ending.

Example 13:

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 572g of the fermentation product compound plant extract composition obtained in the example 5, 7.8g of acetyl hexapeptide-8, 2.6g of palmitoyl pentapeptide-3, 2.08g of pentapeptide-3, 0.78g of kinetin, 78g of tea polyphenol, 20.8g of hydroxydecyl ubiquinone, 104g of colloidal oat flour and 18.2g of tocopherol, and fully and uniformly stirring;

Comparative example 1. centella asiatica extract.

Comparative example 2.34 parts of aesculus hippocastanum extract, 32 parts of thyme extract, 29 parts of aloe vera extract, 26 parts of centella asiatica extract and 8 parts of silybum marianum extract were mixed uniformly to form a composition.

Comparative example 3.23 parts of perilla leaf extract, 21 parts of lantana leaf extract, 18 parts of bidens pilosa leaf extract were mixed well to form a composition.

Comparative example 4.23 parts of perilla leaf extract, 21 parts of lantana leaf extract, 18 parts of spanishneedles triloba extract, 34 parts of aesculus hippocastanum extract, 32 parts of thyme extract, 29 parts of aloe vera extract, 26 parts of centella asiatica extract and 8 parts of silybum marianum extract were mixed uniformly to form a composition.

Comparative example 5.80 parts yeast/grape fermentation extract, 180 parts split yeast fermentation filtrate/lysate/extract, 110 parts alteromonas fermentation extract were mixed together to form a composition.

Comparative example 6.23 parts of perilla leaf extract, 21 parts of lantana leaf extract, 18 parts of bidens pilosa extract, 34 parts of aesculus hippocastanum extract, 32 parts of thymus vulgaris extract, 29 parts of aloe vera extract, 26 parts of centella asiatica extract, 8 parts of silybum marianum extract, 80 parts of yeast/grape fermentation product extract, 180 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract and 110 parts of alteromonas bacterium fermentation product extract were mixed uniformly to form a composition.

Comparative example 7.23 parts of perilla leaf extract, 21 parts of lantana leaf extract, 18 parts of bidens pilosa extract, 34 parts of aesculus hippocastanum extract, 32 parts of thyme extract, 29 parts of aloe vera extract, 26 parts of centella asiatica extract, 8 parts of silybum marianum extract, 80 parts of yeast/grape fermentation product extract, 180 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 110 parts of alteromonas ferment extract, 7 parts of acetyl hexapeptide-8, 3 parts of palmitoyl pentapeptide-3 and 1 part of pentapeptide-3 were mixed uniformly to form a composition.

Comparative example 8. anti-aging repair essence milk prepared according to the following steps:

1) adding 1404g of water into a water phase pot, and adding 158g of the essence milk base component 1 of example 9 under stirring; then stirring and heating to 75-85 ℃ to obtain an aqueous phase solution;

2) adding 139g of the essence emulsion base component 2 of the embodiment 9 into an oil phase pot, and heating to 75-85 ℃ to melt the materials to obtain an oil phase solution;

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 832g of the plant extract in the comparative example 1, and fully and uniformly stirring;

5) adding 41g of the essential milk base component 3 of the example 9 into the material obtained in the step 4); stirring uniformly, standing for 1 hour, continuing stirring for 5-10 minutes, and adjusting the pH value to 6.0-6.5; and (6) ending.

Comparative example 9

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 832g of the plant extract composition of the comparative example 2, and fully and uniformly stirring;

Comparative example 10

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 832g of the plant extract composition of the comparative example 3, and fully and uniformly stirring;

Comparative example 11

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 832g of the plant extract composition of the comparative example 4, and fully and uniformly stirring;

Comparative example 12

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 832g of the plant extract composition of the comparative example 5, and fully and uniformly stirring;

Comparative example 13

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 832g of the plant extract composition of the comparative example 6, and fully and uniformly stirring;

Comparative example 14

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding 832g of the plant extract composition of the comparative example 7, and fully and uniformly stirring;

The detection sample is as follows:

sample i: the essence cream obtained in comparative example 8; sample ii: the essence cream obtained in comparative example 9; sample iii: the essence cream obtained in comparative example 10; sample IV: the essence cream obtained in comparative example 11; sample V: the essence cream obtained in comparative example 12; sample VI: the essence cream obtained in comparative example 13; sample VII: the essence cream obtained in comparative example 14; the following tests were performed:

the first test example: inhibition of MMP-1 expression

One of the main causes of skin aging is the alteration of the structure of the dermis layer. Due to the action of some external factors, Matrix Metalloproteinases (MMPs) in the body are activated, which can cause excessive degradation of collagen and elastin which support the skin structure in the dermis of the skin, thus causing the skin to have aging symptoms such as shrinkage, inelasticity and the like. MMP-1 is also called collagenase-1, and can degrade collagen types I, II, III, VII and X, gelatin and proteoglycan. The anti-aging effects of these samples were evaluated by testing the rate of inhibition of MMP-1 by these samples.

Fibroblasts were seeded into 12-well cell culture plates, each well containing 0.75 × 10⁵Cells were cultured and starved in serum-free medium for 24 hours. Starved cultured cells were washed with PBS and treated with uv light (40 mJ). Then, the test sample was added to the cells 2 times within 48 hours. MMP-1 isolated from the medium was measured using a kit (BIOTRAK, RPN 2610). Evaluating the strength of anti-aging efficacy by calculating the expression inhibition rate of MMP-1. The calculation formula is as follows:

inhibition rate (A-B)/A100%

A, the expression level of MMP-1 after ultraviolet irradiation without adding a test sample

B, adding a test sample and carrying out ultraviolet irradiation on the expression quantity of MMP-1

TABLE 1 inhibition of MMP-1 expression by different test samples

Test sample	Volume (μ l)	Inhibition ratio (%)
			Sample I	50	8.2
Sample II	50	34.7
			Sample III	50	11.6
Sample IV	50	48.3

As can be seen from Table 1, the inhibition rates of 4 samples to be tested on MMP-1 expression are different, but the inhibition rate of the sample IV on MMP-1 expression is obviously higher than that of the other 3 samples to be tested, and the inhibition rate of the sample IV on MMP-1 expression reaches 48.3% under the concentration of 5 mul and is far higher than that of the other 3 samples under the concentration of 50 mul, which indicates that the sample IV has a remarkable inhibition effect on MMP-1 expression and each component has a good synergistic effect.

Test example two: promotion rate of collagen type iii synthesis:

human fibroblasts cultured in DMEM medium containing 10% FBS were seeded in a 24-well plate, and after cell attachment, medium exchange was performed with DMEM medium containing 0.25% FBS and 250 μ M magnesium ascorbyl phosphate, and the above 7 samples to be tested were added, respectively. After three days of culture, the culture medium supernatant was recovered, centrifuged, and the type III collagen in the resulting supernatant was measured.

The evaluation method of the ability of cell-derived collagen type III to biosynthesize was carried out by measuring the content of the terminal peptide (Procollagentype III-peptide: PIII P for short) of collagen type III secreted in the supernatant using "RIA-gnostP III P (PIII P) measurement kit". The strength of the anti-aging effect of these samples was evaluated by the promotion rate of collagen type III synthesis. The calculation formula is as follows:

the promotion rate is (A-B)/B100%

A amount of type III collagen produced after addition of test sample

Type III collagen production amount without addition of test sample

TABLE 2 promotion of collagen type III Synthesis by different test samples

Test sample	Concentration (μ l)	Acceleration Rate (%)
			Sample I	50	11.3
Sample II	50	44.6
			Sample III	50	18.2
Sample IV	50	68.7

As can be seen from Table 2 above, each single functional ingredient has a certain promoting activity but is low in the amount of type III collagen production. The promotion rate of the sample IV is 68.7%, and is obviously improved compared with the former three samples, which shows that after compounding, all functional components in the extract generate synergistic effect, mutual enhancement is realized, the secretion of cells to type III collagen is promoted, the generation amount of the type III collagen is improved, the extracellular matrix is thickened, and further the anti-aging effect is realized.

Test example three: ultraviolet resistance test

TABLE 3 UV protection test results units (%) -for samples V, VI, VII

	Sample V	Sample VI	Sample VII
				UVA absorption rate	34.0	36.0	36.0
UVB absorption rate	47.0	56.0	58.0
				UVC absorption rate	14.3	15.0	15.2

As can be seen from table 3: sample VII the essential milk containing plant extracts, fermentation product extracts and regeneration repair active polypeptides was best uv protected. The anti-ultraviolet capability is mainly detected by using an Fe3+ reduction method, the total antioxidant capability of skin tissues is obviously reduced after a large amount of ultraviolet radiation, and the accumulation of skin tissue lipid peroxidation products caused by ultraviolet radiation is related to the weakening of the effect of a tissue antioxidant defense system.

Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can make modifications and equivalents to the embodiments of the present invention without departing from the spirit and scope of the present invention, which is set forth in the claims of the present application.

Claims

1. The anti-aging repair composition is characterized by comprising a fermentation product extract and a plant extract in parts by weight, wherein the fermentation product extract comprises: 20-120 parts of yeast/grape fermentation product extract, 45-180 parts of secondary fission yeast fermentation product filtrate/lysate/extract and 30-160 parts of alteromonas fermentation product extract; the plant extract comprises: 2-23 parts of perilla leaf extract, 3-21 parts of lantana leaf extract, 2-18 parts of bidens pilosa extract, 5-40 parts of aesculus hippocastanum extract, 5-32 parts of thyme extract, 3-36 parts of aloe barbadensis extract, 3-34 parts of centella asiatica extract and 2-12 parts of silybum marianum extract.

2. The anti-aging composition according to claim 1, wherein the anti-aging composition is composed of, by weight, 80 parts of yeast/grape fermentation product extract, 180 parts of schizosaccharomyces cerevisiae fermentation product filtrate/lysate/extract, 110 parts of alteromonas fermentation product extract, 16 parts of perilla leaf extract, 14 parts of lantana camara leaf extract, 12 parts of bidens pilosa extract, 45 parts of aesculus hippocastanum extract, 18 parts of thyme extract, 29 parts of aloe vera extract, 26 parts of centella asiatica extract, and 10 parts of silybum marianum extract.

3. Use of a composition according to claim 1 or 2 for the preparation of a skin care product.

4. An essence milk for skin anti-aging and repairing, which is characterized by comprising 8-32% by mass of the plant extract composition as claimed in claim 1 or 2, 13-26% by mass of an essence milk base component, and the balance of deionized water.

5. The skin anti-aging essence milk according to claim 4, further comprising a regeneration repair active polypeptide consisting of 0.3-0.7% acetyl hexapeptide-8, 0.1-0.3% palmitoyl pentapeptide-3, and 0.001-0.1% pentapeptide-3, in percentage by mass.

6. The skin-antiaging essence milk according to claim 5, characterized in that the essence milk further comprises, in mass%, an antioxidant active ingredient consisting of 0.02 to 0.06% of kinetin, 2 to 4% of tea polyphenol, 0.5 to 1% of hydroxydecyl ubiquinone, 1 to 5% of colloidal oat flour, and 0.5 to 1.5% of tocopherol.

7. The essence for skin anti-aging repair as claimed in claim 6, wherein the essence further comprises, in mass percent, a microcirculation promotion active ingredient consisting of 0.1-0.3% of arginine, 0.1-1.5% of adenosine, and 0.01-1% of biotin, and a natural moisturizing active factor; the natural moisturizing active factor consists of 0.01-0.08% of sodium hyaluronate and 0.2-0.5% of wild mango kernel fat.

8. The essence for skin anti-aging repair as claimed in claim 7, wherein the essence further comprises tetrahydrocurcumin which is a natural whitening ingredient and is added in an amount of 1% by mass.

9. The skin anti-aging repair essence cream according to claim 4, wherein the essence cream base component is composed of, by weight, 8 parts of glycerin, 1 part of butylene glycol, 1 part of propylene glycol, 1 part of caprylic/capric triglyceride, 1 part of polydimethylsiloxane, 1 part of hydrogenated coco glyceride, 1 part of isononyl isononanoate, 0.2 part of stearyl alcohol, 1 part of cetyl alcohol, 1 part of jojoba esters, 0.2 part of glyceryl stearate, 0.2 part of hydrogenated lecithin, 3 parts of steareth-2, 1 part of steareth-21, 1 part of polymethylmethacrylate, 1 part of acrylic acid/C10-30 alkanol acrylate crosspolymer, 0.2 part of xanthan gum, 1 part of hydroxyethyl cellulose, 0.1 part of titanium dioxide, 0.5 part of phenoxyethanol, 1 part of disodium EDTA, 1 part of chlorphenesin, 1,2 pentanediol, 1 part of ethylhexylglycerin, 1 part of p-hydroxyacetophenone, 1 part of potassium sorbate, 1 part of a colorant CI19140, 1 part of a colorant CI14700 and 1 part of essence.

10. The preparation method of the skin anti-aging repair essence cream according to claims 4 to 9, wherein the preparation method comprises:

4) after heat preservation, opening cooling water, cooling the mixed material obtained in the step 3) to 40-45 ℃, adding the fermentation product compound plant extract anti-aging repair composition, regeneration repair active polypeptide, antioxidant active ingredient, natural moisture-keeping active factor, microcirculation promoting active ingredient and tetrahydrocurcumin, and fully and uniformly stirring;