CN105769944A - Moringa oleifera extract with weight reducing effect, as well as preparation method and use of moringa oleifera extract - Google Patents
Moringa oleifera extract with weight reducing effect, as well as preparation method and use of moringa oleifera extract Download PDFInfo
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- CN105769944A CN105769944A CN201610140533.9A CN201610140533A CN105769944A CN 105769944 A CN105769944 A CN 105769944A CN 201610140533 A CN201610140533 A CN 201610140533A CN 105769944 A CN105769944 A CN 105769944A
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- moringa oleifera
- moringa
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- 235000011347 Moringa oleifera Nutrition 0.000 title claims abstract description 41
- 239000000284 extract Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 230000001603 reducing effect Effects 0.000 title abstract description 4
- 244000179886 Moringa oleifera Species 0.000 title abstract 9
- 210000000577 adipose tissue Anatomy 0.000 claims abstract description 10
- 239000006228 supernatant Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000220215 Moringa Species 0.000 claims description 32
- 238000000034 method Methods 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 10
- 208000008589 Obesity Diseases 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 235000020824 obesity Nutrition 0.000 claims description 7
- 235000013402 health food Nutrition 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 5
- 235000013361 beverage Nutrition 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000002137 ultrasound extraction Methods 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 8
- 235000019197 fats Nutrition 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 7
- 201000010063 epididymitis Diseases 0.000 description 5
- 210000003486 adipose tissue brown Anatomy 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 210000000918 epididymis Anatomy 0.000 description 4
- 210000002468 fat body Anatomy 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 208000004611 Abdominal Obesity Diseases 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 2
- 206010065941 Central obesity Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 206010033307 Overweight Diseases 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 210000002216 heart Anatomy 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 238000001543 one-way ANOVA Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 101100154912 Mus musculus Tyrp1 gene Proteins 0.000 description 1
- 101100102907 Mus musculus Wdtc1 gene Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000002269 analeptic agent Substances 0.000 description 1
- 230000003555 analeptic effect Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000021409 diet quality Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 230000010247 heart contraction Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002483 medication Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 208000008338 non-alcoholic fatty liver disease Diseases 0.000 description 1
- 208000019180 nutritional disease Diseases 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 208000019116 sleep disease Diseases 0.000 description 1
- 208000020685 sleep-wake disease Diseases 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
The invention discloses moringa oleifera extract with a weight reducing effect, as well as a preparation method and use of the moringa oleifera extract. The preparation method for the moringa oleifera extract comprises the following steps: impregnating moringa oleifera in water, performing ultrasonic extraction, concentration and centrifugation, extracting supernatant, and performing drying to obtain the moringa oleifera extract. The moringa oleifera extract and the preparation method and use thereof have the advantages that the obtained moringa oleifera extract has a remarkable weight reducing effect, and body fat can also be remarkably reduced.
Description
Technical field
The invention belongs to pharmaceutical technology field, particularly to a kind of Moringa extract with weight losing function and preparation method thereof and purposes.
Background technology
Obesity is the chronic nutritional disease of one that the energy metabolism disequilibrium of body causes, looks about the whole world, and each country of obese people number is raising.U.S. adults has 66% to be overweight or fat, and incidence of obesity is 32%.And in China, end 2009, male overweight ratio has reached 11.4%, and women has reached 10.1%, male's abdominal obesity ratio 27.8%, women abdominal obesity 45.9%.Research shows that obesity can be caused by many factors such as gene, diet, environment, tradition, is the predisposing factor of the multiple diseases such as non-alcohol fatty liver (NAFLD), type Ⅱdiabetes mellitus, atherosclerosis (AS), tumor, sleep disorder, osteoarthritis.So finding safely and effectively appetrol to become the study hotspot of pharmaceutical sanitary field.
The each several part of Moringa such as leaf, root, seed, bark, fruit, flower and immature soybean pod etc. can be used as medicine, it is the analeptic of heart and blood circulation, there is blood fat reducing, blood pressure lowering, blood sugar lowering, antitumor, antiinflammatory, antioxidation, protect the liver and protect the functions such as kidney, antibacterial and antiviral.Multiple enzyme component in Moringa participates in metabolism, can maintain heart, kidney, muscle, neural normal function, also can antioxidation, change bacterioflora ecology.American-European-Japanese emerging health food waiting country and Taiwan has been become in Moringa tree in 2013, its feature is high calcium, high protein, high microsteping and low lipid, becomes people and improves life and diet quality, enhancing immunity, promote healthy, prophylactic magical plant.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of significant Moringa extract of fat-reducing effect.
In order to solve above-mentioned technical problem, the technical scheme is that a kind of method preparing Moringa extract, comprise the following steps: Moringa is soaked in water, supersound extraction, concentration, centrifugal, take supernatant and dry, obtain Moringa extract.
As preferably, the time of described immersion is 10～60 minutes.
As preferably, the time of described supersound extraction is 40 minutes, and the number of times of extraction is 3 times.
As preferably, the described cryodesiccated mode of dry employing carries out.
The present invention also provides for the application in preparation treatment and/or the pre-medicine of preventing obesity, food, beverage or health food of a kind of described Moringa extract.
The present invention also provides for a kind of described Moringa extract and reduces the application in the medicine of body fat, food, beverage or health food in preparation.
Employing Moringa soaks, and supersound extraction gained Moringa extract has significant fat-reducing effect.
Accompanying drawing explanation
Fig. 1 is the Moringa extract of the present invention impact on normal Mouse Weight；
Fig. 2 is the Moringa extract of the present invention impact on normal mouse brown fat and weight ratio；
Fig. 3 is the Moringa extract of the present invention impact on normal mouse epididymis fat and weight ratio；
Fig. 4 is the Moringa extract of the present invention impact on normal mouse intestinal system's fat and weight ratio.
Detailed description of the invention
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described further.At this it should be noted that be adapted to assist in for the explanation of these embodiments and understand the present invention, but it is not intended that limitation of the invention.As long as just can be mutually combined additionally, technical characteristic involved in each embodiment of invention described below does not constitute conflict each other.
Embodiment 1
A kind of method preparing Moringa extract, comprises the following steps: by Moringa coarse crushing, by the distilled water immersion 30 minutes of 10 times of weight, supersound extraction 3 times, extract 40 minutes every time, filter, filtrate is concentrated, centrifugal, take supernatant and carry out lyophilization, obtain Moringa extract.
Embodiment 2
A kind of method preparing Moringa extract, comprises the following steps: by Moringa coarse crushing, by the distilled water immersion 10 minutes of 12 times of weight, supersound extraction 3 times, extract 40 minutes every time, filter, filtrate is concentrated, centrifugal, take supernatant and carry out lyophilization, obtain Moringa extract.
Embodiment 3
A kind of method preparing Moringa extract, comprises the following steps: by Moringa coarse crushing, by the distilled water immersion 60 minutes of 8 times of weight, supersound extraction 3 times, extracts 40 minutes every time, filters, filtrate concentrated, centrifugal, takes supernatant and carries out lyophilization, obtains Moringa extract.
For verifying the technique effect of the present invention, spy makes tests below:
1 material and method
1.1 test samples
Embodiment gained Moringa extract
1.2 laboratory animals
The male Kunming mouse of SPF level 45, body weight 20～25g.
1.3 animal packets
By body weight random packet, respectively matched group (normal saline), low dose group (0.5g/ days) and 3 groups of high dose group (1g/ days).
1.4 medications, gavage, administration time is 10 weeks.
1.5 index observings
1.5.1 obesity index is observed
Whether normal every day entry mice food-intake during administration, observe the appetite of mice, stool, urine volume, drinking-water, fur, activity, spirit, eyes, heart beating.Every day, timing was weighed in 1 time.Dissect mice, take epididymal adipose tissues, intestinal system fat and brown fat weighing, i.e. fat pad weight in wet base, calculate body fat ratio.
1.6 statistical method
Above experimental data processes through Graphpad statistical software, and experimental result adopts means ± SD to represent, application One-WayANOVA carries out the comparison organizing sample average more, and P < 0.05 represents statistically significant.
2 interpretations of result
2.1 impacts on obesity index
2.1.1 Mouse Weight change
During administration, every day regularly weighs in 1 time, and data process through Graphpad statistical software, and application One-WayANOVA carries out the comparison organizing sample average more.It is shown that as shown in Figure 1, low dose group Mouse Weight reduces compared with the control, but there was no significant difference；High dose group Mouse Weight significantly reduces compared with the control.
2.1.2 the change of mouse adipose pad and body fat ratio
After administration terminates, dissect mice, take epididymis, intestinal system and brown fat, calculate fat pad weight in wet base and body fat ratio, observing the impact on Mice Body fat of each medicine, shown in result: as shown in Figure 2, the fat body of high dose group mice brown fat reduces than significance compared with normal group and matched group；From the figure 3, it may be seen that the fat body of high dose group mouse epididymis fat reduces than significance compared with normal group and matched group, the fat body of low dose group mouse epididymis fat reduces than significance compared with normal group；As shown in Figure 4, the fat body of high dose group mice intestinal system fat reduces than significance compared with normal group and matched group.
Above in association with accompanying drawing, embodiments of the present invention are explained in detail, but the invention is not restricted to described embodiment.For a person skilled in the art, when without departing from the principle of the invention and spirit, these embodiments are carried out multiple change, amendment, replacement and modification, still falls within protection scope of the present invention.
Claims (6)
1. the method preparing Moringa extract, it is characterised in that: comprise the following steps: Moringa is soaked in water, supersound extraction, concentration, centrifugal, take supernatant and dry, obtain Moringa extract.
2. method according to claim 1, it is characterised in that: the time of described immersion is 10～60 minutes.
3. method according to claim 1, it is characterised in that: the time of described supersound extraction is 40 minutes, and the number of times of extraction is 3 times.
4. method according to claim 1, it is characterised in that: the described cryodesiccated mode of dry employing carries out.
5. Moringa extract application in preparation treatment and/or the pre-medicine of preventing obesity, food, beverage or health food according to claim 1.
6. Moringa extract reduces the application in the medicine of body fat, food, beverage or health food in preparation according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610140533.9A CN105769944A (en) | 2016-03-11 | 2016-03-11 | Moringa oleifera extract with weight reducing effect, as well as preparation method and use of moringa oleifera extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610140533.9A CN105769944A (en) | 2016-03-11 | 2016-03-11 | Moringa oleifera extract with weight reducing effect, as well as preparation method and use of moringa oleifera extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105769944A true CN105769944A (en) | 2016-07-20 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610140533.9A Pending CN105769944A (en) | 2016-03-11 | 2016-03-11 | Moringa oleifera extract with weight reducing effect, as well as preparation method and use of moringa oleifera extract |
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| CN (1) | CN105769944A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304630A (en) * | 2021-12-24 | 2022-04-12 | 中国热带农业科学院热带生物技术研究所 | Rapid preparation method and application of moringa oleifera total flavonoids |
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Application publication date: 20160720 |