CN109757637A - Functional beverage containing birch young pilose antler extract - Google Patents
Functional beverage containing birch young pilose antler extract Download PDFInfo
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Abstract
The present invention relates to a kind of functional beverages, wherein, containing birch young pilose antler extract, the present invention also provides a kind of functional beverage conditioning chronic disease, adjust endocrine, summary sleep quality, hyperthyroidism, beautifying face and moistering lotion, improve immunity, conditioning digestive system, blood pressure lowering, hypoglycemic, reducing blood lipid, it is anti-oxidant, remove free radical, eliminate bad breath, constipation, the application in pre- anti-cancer.
Description
Technical field
The invention belongs to a kind of functional beverages containing birch young pilose antler extract, the especially function containing birch young pilose antler extract
Can property beverage conditioning chronic disease, adjust endocrine, improve sleep quality, hyperthyroidism, beautifying face and moistering lotion, improve immunity, conditioning disappear
Change system, blood pressure lowering, hypoglycemic, reducing blood lipid, it is anti-oxidant, remove free radical, eliminate bad breath, constipation, the application in pre- anti-cancer.
Background technique
In recent years, due to environmental pollution, living habit changes (not eating coarse food grain) and work rhythm is accelerated, and lacks many people
Nutrition is in sub-health state such as biologically active peptide, microelement and multivitamin.If continuing to develop, disease will be changed into
Disease.Diabetes, hypertension, tumour are high-risk in high-risk again in inferior health people at highest risk, are such as taken measures not in time, these diseases
Disease can be come at any time on everyone body.
The country is limited to cross-section survey to the research of inferior health more, and the tool used is mostly Self-assessment Scale or questionnaire.
These investigation are related to the different crowds such as teacher, civil servant, enterprise personnel, community resident, medical worker.Since each research uses
Inferior health define disunity, the questionnaire of application or scale disunity, the inferior health recall rate difference of each research report
It is larger, mostly between 20%-80%.The recall rate of inferior health different sexes, the age, occupation on have different, with go out
Radix Rehmanniae, nationality are unrelated.The recall rate of general women is higher than male, and 40-50 years old age bracket is high-incidence compared with other age brackets, teacher, public affairs
Business person is high-incidence.The main reason for leading to inferior health has: diet is unreasonable, lacks movement, work and rest irregular, sleep insufficiency, spirit
It is nervous, psychological pressure is big, long-term unhealthy emotion etc..
The clinical manifestation of inferior health is varied, can behave as fatigue and weak, muscle and joint pain, giddy in terms of body
Headache, palpitaition is uncomfortable in chest, sleep disordered, loss of appetite, stomach duct and abdomen discomfort, loose stool constipation, hypogona dism, cold To Be Protected from Heat, is easy to feel
It emits, eye is dry and astringent etc.;Psychological aspects can express be in a bad mood it is low, upset, lather, being irritable and getting angry easily, frightened timid, note
Recall power declines, attention cannot be concentrated, is deficient in energy, is slow in reacting etc.;Can express in terms of social interaction has and cannot preferably hold
Corresponding social role is carried on a shoulder pole, work, difficulty of learning cannot normally handle interpersonal relationships, family relationship well, it is difficult to carry out normal
Social interaction etc..
Inonotus obliquus (Inonotus obliquus) is distributed mainly on Heilongjiang Province, China and Jilin Province;In external main product
In countries such as Russia, Finland, Poland.Inonotus obliquus contains polysaccharide, Inonotus obliquus alcohol, triterpene compound, lanosterol etc.,
With a variety of biological effects.The effects of mainly including hypoglycemic, anticancer, anti-inflammatory, anti-oxidant, blood pressure lowering;It is adjusted with good
Hormone normal secretions improve endogenous estrogen secretion, improve sleep, relax bowel and defecation, reduce cholesterol, alleviate anxiety, suppression
Strongly fragrant equal effect.The former Soviet Union just announces that Inonotus obliquus is cancer-resisting substance in nineteen fifty-five;It is " special that Inonotus obliquus is also included in by the U.S.
Natural materials ";It is Japanese then using Inonotus obliquus as the therapeutic agent of liver cancer, AIDS etc., and applied for multinomial related patents.
Cepe (Boletus edulis) is distributed more widely in China, and because fleshy hypertrophy, pole is gained the name like beef liver.It is main
Originate in the ground such as Southwestern China, East China, northeast;Foreign countries are distributed mainly on Europe, North America, Japan, Australia etc..Cepe
It is full of nutrition, rich in protein, fat, crude fibre, a variety of mineral elements and vitamin etc..Cepe contains phenols, Huang
Ketone, polysaccharide, terpene and other effects composition have anti-oxidant, antifatigue, antitumor, strengthen immunity and other effects.
Collybia albuminosa (Termitomyces albuminosus Heim) be distributed mainly on Southwestern China, the several provinces in the southeast and
The areas such as Taiwan;Foreign countries are concentrated mainly on the torrid zones, South Asia, the subtropical zones such as African south, the flat island in East Asia and South Pacific.Collybia albuminosa bacterium
Meat is delicate, Filamentous in muscle, and mouthfeel is fresh and sweet, faint scent, and because having certain similarity with chicken on taste, therefore gains the name.Chicken
Protein content is up to 36.94% in fir bacterium dry product;Unsaturated fatty acid is advantage fatty acid, based on linoleic acid, oleic acid;
Potassium, sodium content ratio are 5.30, are conducive to maintain body acid-base balance and normal arterial pressure;Amino acid profile Glutamic Acid, asparagus fern
The delicious amino acids content such as propylhomoserin is much higher than other amino acid, has delicate flavour outstanding.Saponin(e also rich in collybia albuminosa,
Phenols, polysaccharide, ergosterol isoreactivity ingredient have enhancing immune function of human body, antitumor, enriching the blood to restore normal menstruation, strengthening the spleen and stomach etc. more
Kind of pharmacological action, especially anticancer, it is hypoglycemic, in terms of there is unique physiological function.
China is big country of mushroom producing, about 30,000,000 tons of annual total output, 3/4ths or more of Zhan Quanqiu total output,
Occupy the first in the world.Mainly there are snack food, functional food, flavouring and cosmetics currently with the product that edible mushroom is developed
Deng;The main series such as nutritious oral solution, health drink, health protection tea, health-care tonic wine, health-caring capsule of functional food.
Currently, having one by the Patent Domestic that beverage application is made in raw material of collybia albuminosa." a kind of collybia albuminosa beverage and its system
Preparation Method ", (CN104351899A);2015-02-18 (publication date).It is characterized in that it is using collybia albuminosa as raw material, through heat
Scald, mashing, filtering, allotment, homogeneous, degassing, it is filling, sterilize prepared by;Finished product is in yellowish-brown, and solid content is not less than
10%, total acid < 1.0%;With the distinctive fragrance of collybia albuminosa and flavour, it is not susceptible to precipitated and separated, typicalness is prominent.
It is that numerous researchers set about studying liquid using collybia albuminosa mycelium developing food products, drug and the certain functional components of extraction
The original intention of body fermentation collybia albuminosa.It can reach large-scale production using mycelium development, but it developed and used not now
It is widely available.Existing research is mainly using some common edible mushrooms as the product of Raw material processing, such as: with the health care of Edible mushroom processing
The glossy ganoderma health-care tea beverage that beverage, especially ganoderma lucidum are prepared is preferable in American-European and Japanese market;Using edible mushroom as raw material, phase is added
Answer auxiliary material, the health liquor of unique flavor made of fermented brew;With edible mushroom extract production flavouring and with eat
Preserved fruit, preserved fruit made of bacterium, jam product or as typical local food such as cake, bread made of auxiliary material etc..Therefore, collybia albuminosa is ground
The exploitation studied carefully and products thereof has very wide application prospect.
Based on this, country has proposed big fitness program, that is, payes attention to prevention, preventives treatment of disease.Currently, edible function food is most
Popular health care mode.China just proposes the idea of " Diet cures more than the doctors " from ancient times, and functional food is healthy and safe, nutrition is protected
It is strong, i.e., it is convenient again convenient for receiving, it is free from side effects, or even can play the role of drug and not have.
Summary of the invention
The present invention is in view of the above project, after inventor concentrates on studies, provides following technical scheme, the i.e. purpose of the present invention
It is to provide a kind of functional beverage, wherein contain birch young pilose antler extract.
Functional beverage of the present invention, wherein the birch young pilose antler is to originate from the Siberian birch young pilose antler of Russia.
Functional beverage of the present invention, wherein also contain collybia albuminosa extract.
Functional beverage of the present invention, wherein the collybia albuminosa is the collybia albuminosa for originating from Yunnan.
Functional beverage of the present invention, wherein also contain Porcini extract.
Functional beverage of the present invention, wherein the bolete is the bolete for originating from Yunnan.
Functional beverage of the present invention is in conditioning chronic disease, adjusting endocrine, summary sleep quality, hyperthyroidism, beauty
Beauty treatment, improve immunity, conditioning digestive system, blood pressure lowering, hypoglycemic, reducing blood lipid, it is anti-oxidant, remove free radical, eliminate bad breath, just
Application in secret, pre- anti-cancer.
Functional beverage set of the invention birch young pilose antler, collybia albuminosa, bolete essence, for people improve inferior health and
Skin maintenance and cosmetic benefits provides best selection.Functional beverage of the invention is conducive to anti-oxidant, removing interior free yl, and enhancing is immune
Power, pre- anti-cancer.Make body rejuvenation, long-term drinking functional beverage of the invention can have a bath to your blood, can be effective
Hypoglycemic, reducing blood lipid and prevent hypertension.Functional beverage of the invention is to endocrine disturbance, hepatitis, gastritis, duodenum
Ulcer, abdominal pain, gastrointestinal dysfunction also have apparent opsonic action, and long-term drinking functional beverage of the invention can strong body
Body, makes that the viscera function of human body is vigorous, qi and blood enriches, resist and prevents germ from invading.Through the invention, it is slow to include at least conditioning
Venereal disease adjusts endocrine, summary sleep quality, hyperthyroidism, beautifying face and moistering lotion, improves immunity, conditioning digestive system, blood pressure lowering, drop
Blood glucose, reducing blood lipid, it is anti-oxidant, remove free radical, eliminate bad breath constipation, pre- anti-cancer.
Detailed description of the invention
Fig. 1 shows functional beverage DPPH Scavenging activity
Fig. 2 shows the ABTS Scavenging activities of functional beverage
Fig. 3 shows functional beverage to Hydroxyl radical-scavenging ability
Fig. 4 shows the total reducing power of functional beverage
Fig. 5 shows functional beverage total antioxidant capacity
Specific embodiment
In order to which the present invention becomes effective technological means, illustrate feature of the invention with the following Examples.It is specific real
Applying mode only is exemplary explanation, rather than limiting the invention, for those of ordinary skills, can be according to upper
State it is bright be improved or converted, and all these modifications and variations all should belong to appended claims of the present invention.
Specifically, the present invention provides a kind of functional beverage, wherein contain birch young pilose antler extract.It is preferred that the birch
Young pilose antler is fine and soft to originate from the Siberian birch of Russia.
Functional beverage of the present invention, wherein also contain collybia albuminosa extract.It is preferred that the collybia albuminosa is to originate from cloud
The collybia albuminosa in south.
Functional beverage of the present invention, wherein also contain Porcini extract.It is preferred that the bolete is to originate from cloud
The bolete in south.
Functional beverage of the present invention is in conditioning chronic disease, adjusting endocrine, summary sleep quality, hyperthyroidism, beauty
Beauty treatment, improve immunity, conditioning digestive system, blood pressure lowering, hypoglycemic, reducing blood lipid, it is anti-oxidant, remove free radical, eliminate bad breath, just
Application in secret, pre- anti-cancer.
Embodiment
The present invention is prepared in accordance with the following methods, as follows in detail:
1. raw material preparation: Inonotus obliquus (birch is fine and soft) being ground into 1~2cm size shape, without cleaning;Collybia albuminosa is dry
Good monolith, flowing water cleaning is secondary, spare;The good bolete flowing water cleaning of chip drying is secondary, spare.
Extraction is prepared with nanometer: tap water is handled by double-layer nanometer filter plant.
2. Inonotus obliquus and treated nanometer water are first put into extraction with the ratio of solid-liquid ratio 1: 30 (mass ratio)
In tank, extracted for the first time under the conditions of 100 DEG C of temperature, extraction time 3h;After extraction, supernatant is using vacuum drawn to dense
It is spare in contracting tank;Filter residue continues second of extraction.
3. the Inonotus obliquus filter residue after first time is extracted carries out second using nanometer water with the ratio of 1: 30 (mass ratio)
Secondary extraction, Extracting temperature are 100 DEG C, extraction time 2h;After extraction, supernatant utilizes vacuum drawn into concentration tank,
Merge with first time extracting solution;Filter residue continues third time and extracts.
4. the Inonotus obliquus filter residue after second of extraction carries out third time together with the ready collybia albuminosa of cleaning, bolete
It extracts, three kinds of material quality ratios are 2: 1: 1;The amount that nanometer water is added is 1: 30 (mass ratio).Extracting temperature is 100 DEG C, is mentioned
Taking the time is 1h;After extraction, supernatant extracts supernatant using vacuum drawn to concentration tank, with first and second Inonotus obliquus
Liquid merges.
5. the extracting solution three times of merging is concentrated under reduced pressure, until the one third of total volume.
6. the extracting solution after concentration is centrifuged, 14000rpm, 30~60min.
7. by after centrifugation supernatant and nanometer water deployed with volume 1: 1, process need to stir 30min.
8. deployed extracting solution is carried out filling;120 DEG C of sterilizing 30min are carried out again;As finished product.
Effect and technique is further described by measurement functional component content in the present invention.
One) measurement of general flavone content
The drafting of general flavone standard curve: 5 mg of rutin standard items of 120 DEG C of dryings to constant weight is weighed, volume fraction is used
70% ethyl alcohol dissolves and is settled to 25mL, accurately draw 0.0,0.1,0.2,0.3,0.4,0.5,0.6,0.7,0.8,0.9,
1.0mL is placed in test tube, and insufficient section is supplemented to 2mL with 70% ethyl alcohol of volume fraction, adds 5% NaNO2Solution 0.3mL, shakes
Even, standing 6min, adds 10%Al (NO3)3Solution 0.3mL shakes up, stands 6min, finally plus 4%NaOH solution 2mL, shakes
Absorbance is surveyed under 510nm wavelength after even, standing 10min.Using the quality of rutin standard items as abscissa, absorbance is ordinate
Standard curve is drawn, equation Y=0.0029X+0.0052 (R2=0.9983) is obtained.
The measurement and calculating of content: accurate pipette samples liquid 1mL adds 70% ethyl alcohol 1mL of volume fraction, by standard song
Line preparation step measures absorbance, and the content of test sample rutin is read from standard curve.
Two) measurement of total phenol content
Total phenol content is measured using Folin- phenol method.Gallic acid reference substance 5mg is accurately weighed, is dissolved and is determined with distilled water
Hold into 50mL volumetric flask, obtains the gallic acid standard solution that concentration is 0.1mg/mL.Pipette 0 respectively, 1.0,2.0,3.0,
4.0,5.0,6.0, the gallic acid standard solution of 7.0mL is in the volumetric flask of 10mL, and adds distilled water constant volume, obtain 0,10,
20, the serial gallic acid standard solution of 30,40,50,60,70 μ g/mL.Respectively from above-mentioned serial gallic acid standard solution
1.0mL solution is drawn, respectively plus 5 mL of distilled water, forint phenol reagent 1mL, 7.5g/100mL Na2CO3 solution 3mL, oscillation are aobvious
After color one hour, the absorbance of series standard solution is measured under 760nm wavelength.Standard is drawn by standard sample of gallic acid
Curve obtains regression equation: Y=0.006 X-0.0428 (R2=0.9948).
1.0mL sample solution is drawn in test tube, respectively plus distilled water 5mL, forint phenol reagent 1mL, 7.5g/100mL
Na2CO3 solution 3mL measures the absorbance of solution under 760nm wavelength, reads from standard curve after oscillation colour developing one hour
The content of test sample gallic acid out.1.2.1.3 the measurement (phend-sulphuric acid) of Thick many candies content
Using phend-sulphuric acid, using DEXTROSE ANHYDROUS as standard items, precision measure reference substance solution 0.2,0.4,0.6,
0.8,1.0mL is respectively placed in tool plug test tube, adds water to 3.0mL respectively, and 5% phenol solution 1mL of each accurate addition shakes up, fast
Sulfuric acid 5mL is added in speed, shakes up, and places 10min, sets and keep the temperature 20min in 40 DEG C of water-baths, cold bath 10min after taking-up, with corresponding
Reagent is blank.By UV-VIS spectrophotometry, absorbance is measured at 490nm, using absorbance as ordinate, concentration is
Abscissa draws standard curve, and acquires regression equation.Obtaining standard curve regression equation is Y=9.1397X+0.0023, R2=
0.9912。
20mL sample is measured respectively, it is each that 80mL dehydrated alcohol (ratio for guaranteeing sample and dehydrated alcohol is 1: 4) is added, in
One night of alcohol precipitation in 4 DEG C of refrigerators takes out 1 h that flows back in 80 DEG C of water-baths, cleans flask, centrifugation with 30 milliliters of hot dehydrated alcohols
(4000rmp) 10min, discards supernatant liquid, and floccule is transferred in flask again, 150mL distilled water is added, in 80 DEG C of water-baths
Middle reflux 2h, transfer is settled to 250mL to get test sample is arrived respectively.
The above-mentioned test sample of 1.0mL is drawn respectively in test tube, and step is the same as standard curve determination method.It carries out repeating to try three times
It tests.Purity of polysaccharide, unit g/100mL is calculated according to the following formula.
In formula: total starches content is in terms of DEXTROSE ANHYDROUS in X- sample, g/100mL;
C- is acquired the amount of glucose in detected solution, mg/mL by standard curve;
V- sample constant volume, mL;
M- sample sampling amount, mL.
Three) measurement of adenosine content
Chromatographic column:C18 (4.6mm × 150mm, 5 μm), 0.8 mL/min of flow velocity, Detection wavelength
260nm, 10 μ L of sample volume, column temperature: 30 DEG C, mobile phase A: 15% methanol, Mobile phase B: 85% water, Isocratic clution.Essence
It is close to weigh adenosine standard items, with flowing phased soln, and constant volume in volumetric flask be used as reference substance solution, draw a certain amount of mistake 0.45
μm filter membrane;1mL sample is drawn, 0.45 μm of filter membrane is crossed, carries out sample introduction.And it is calculated with following equation.Y=173075x-
24015;R2=0.9972.
The comparison of 1 DPPH Scavenging activity of experimental example
Accurately weigh 4mg DPPH be dissolved in dehydrated alcohol and constant volume with to 100mL, with obtaining 100 umol/L DPPH work
Liquid;Sample liquid is subjected to 1 times, 10 times, 50 times of dilution respectively on the basis of preliminary experiment;DPPH work is added in sample liquid 1mL
Liquid 3mL shakes up, is protected from light after standing 30min and surveys absorbance at wavelength 517nm as Ai;It is added with 1mL distilled water instead of sample liquid
It is A0 that DPPH working solution 3mL, which measures absorbance,;It is Aj that distilled water solution 3mL is added in sample liquid 1mL and measures absorbance.By following
Formula calculates clearance rate.It is control with Vc.
DPPH is a kind of stable free radical in organic solvent, and color is in aubergine, has strong suction under 517nm wavelength
It receives.It can be matched with the lone pair electrons of DPPH in the presence of having radicals scavenging substance in system, absorbing will weaken or disappear, and
Absorb the activity of the degree reflection radicals scavenging substance weakened.Preliminary experiment finds sample stoste to the clearance rate of DPPH free radical
It is very high, therefore also investigated when sample dilutes 1 times, 10 times and 50 times to the elimination effect of DPPH free radical, and and 0.05mg/
MLVc is compared.As seen from Figure 1, sample stoste has good DPPH Scavenging activity;When 1 times and 10 times of dilution
When be still able to maintain good elimination effect;When diluting 50 times, Scavenging activity just has apparent decrease.
The comparison of 2 ABTS+ Scavenging activity of experimental example
Prepare the potassium persulfate solution of the ABTS+ and 2.45mmol/L of 7mmol/L, avoid light place after isometric mixing
16h matches to obtain ABTS stock solution;Using it is preceding with dehydrated alcohol by stock solution be diluted to the absorbance 2.00 under 734nm wavelength ±
0.10 ABTS+ working solution.Sample liquid is subjected to 1 times, 10 times, 50 times of dilution respectively on the basis of preliminary experiment, takes ABTS+
Working solution 3mL and sample liquid 0.3mL is measured at wavelength 734nm rapidly after reacting at room temperature 6min, and measuring absorbance is A sample
Product;Same method takes ABTS+ working solution 3mL and distilled water 0.3mL, and measuring absorbance is A blank;Take distilled water 3mL and sample
Liquid 0.3mL measures absorbance as A control, calculates clearance rate by following equation.
ABTS+ free radical is a kind of free radical generated by potassium persulfate oxidation ABTS+.There are hydrogen confessions in system
When the antioxidant of body, ABTS+ free radical can occur reduction reaction and fade, lighter.Preliminary experiment finds sample stoste
It is also very high to the clearance rate of ABTS+ free radical, therefore it is free also to have investigated ABTS+ when sample dilutes 1 times, 10 times and 50 times
The elimination effect of base, and compared with 5mg/mLVc.
As seen from Figure 2, sample stoste and dilution 1 times when all have preferable ABTS+ Scavenging activity, with 5mg/
MLVc elimination effect is suitable;When being diluted to 10 times, clearance rate decline, but still it is able to maintain preferable elimination effect.
The comparison of 3 Hydroxyl radical-scavenging ability of experimental example
Using salicylic acid method, sample liquid is subjected to 1 times, 10 times, 50 times of dilution respectively on the basis of preliminary experiment, respectively
Stoste and various concentration sample liquid 1mL are drawn, 9 mmol/L FeSO of 1mL is added4, 1mL 9mmol/L salicylic acid-ethyl alcohol,
1mL 8.8mmol/L H is added in reaction system2O2Starting reaction, keeps 30min, is returned to zero with distilled water, at room temperature in wavelength
It is As that absorbance is measured at 510nm;Sample liquid is replaced to measure absorbance as A0 with solvent;H2O2 solution is replaced to measure suction with solvent
Luminosity is Ax.Clearance rate is calculated by following equation.
Salicylic acid method is to utilize hydrogen peroxide and Fe2+OH is generated after mixing, and is added to the salicylic acid in reaction system
OH can be effectively captured, and color products can be generated, which has strong absorption at 510 nm of wavelength;If the sample liquid tool being added
Play the role of removing OH, OH will be fought for salicylic acid, reduce the production quantity of color products, lighter.In pre- reality
On the basis of testing, the mass concentration close to 100% is reached as Cmin to DPPH clearance rate using 0.5mg/mLVc, is finally determined
Functional beverage after diluting 1 times, 10 times, 50 times with functional beverage stoste and by stoste is tested.It can be with by experiment
It was found that it is 39.3% that this kind of functional beverage stoste is to Hydroxyl radical-scavenging ability;When extension rate increases, elimination effect
Also weaken;When being diluted 50 times, Scavenging activity is almost nil.
The comparison of the total reducing power of experimental example 4
Using potassium ferricyanide method, on the basis of preliminary experiment, it is minimum dense for being reached with the absorbance of Vc close to 2 mass concentration
Degree.Pipette samples liquid 2mL respectively, sequentially adds the 1% iron cyaniding of phosphate buffer solution and 2mL of 2mL 0.2mol/L pH 6.6
6 solution of potassium K3Fe (CN) mixes, and cools down rapidly after 50 DEG C of holding 20min, adds the solution of trichloroacetic acid of 2mL 10%,
Oscillation therefrom takes 2mL mixed solution after mixing, and respectively plus the liquor ferri trichloridi of the distilled water of 2mL and 0.4mL 0.1%, stands
10min, it is A sample that absorbance is measured at wavelength 700nm;Replacing sample solution to measure absorbance with distilled water is A blank.
Potassium ferricyanide method is to make the Fe3 in iron ion-potassium ferricyanide compound when there is antioxidant in reaction system
+ it is transformed into Fe2+, Fe2+ compound has absorption maximum at wavelength 700nm, and absorbance value shows that more greatly reducing power is stronger.?
It is control with the total reducing power of Vc on the basis of preliminary experiment, after diluting l times, 10 times, 50 times with functional beverage stoste and by stoste
Functional beverage tested.Experiment is it can be found that the total reducing power of this kind of functional beverage is very strong, with 0.3mg/mLVc
Total reducing power it is suitable.The functional beverage and stoste absorbance of one times of dilution are almost without difference;Equally, when 10 times of dilution
When, total reducing power can also die-off.
The comparison of 5 total antioxidant capacity of experimental example
With Sulphuric acid, sodium phosphate and ammonium molybdate mixed solution, make each Reagents Final Concentration be respectively 0.6 mol/L sulfuric acid,
28mmol/L sodium phosphate and 4mmol/L ammonium molybdate;Sample liquid is subjected to 1 times, 10 times, 50 times dilute respectively on the basis of preliminary experiment
It releases, draws various concentration sample liquid 0.4mL respectively, add the above-mentioned mixed solution of 4.0mL, shaken well, 95 DEG C of water-bath 90min
Absorbance value is measured after cooling down afterwards at 695nm.Replace sample liquid as blank group using distilled water.
Under acid condition, Mo6+ can be reduced into Mo5+ by anti-oxidation active substance, and Mo5+ and phosphoric acid form phosphoric acid-again
The green compounds of Mo5+;Color is deeper, and absorbance is bigger, illustrates that sample liquid antioxidant activity is higher.On the basis of preliminary experiment,
Mass concentration when using Vc absorbance close to 2 is final to determine with functional beverage stoste and stoste is diluted 1 as Cmin
Times, 10 times, the functional beverage after 50 times tested.As seen from Figure 5, this kind of functional beverage has very strong
Total antioxidant capacity, wherein the total antioxidant capacity of stoste is strong compared with 0.5 mg/mL Vc's.With the increase of extension rate, function
The total antioxidant capacity of energy property beverage is in the trend gradually weakened.
Every group of experiment is repeated 3 times, and is as a result expressed as means standard deviation, and SPSS19.0 carries out statistical analysis to data.
Functional component content in functional beverage
Effect component content in sample is determined, the results are shown in Table 1.As shown in Table 1, total in the functional beverage
Phenol, general flavone and Thick many candies content are higher;Measurement result shows also to contain efficacy factor adenosine in sample liquid.
Functional component content in 1 functional beverage of table
Total phenol, general flavone, Thick many candies content are higher in the functional beverage, also contain efficacy factor adenosine.This seminar
Measure general flavone content in functional beverage are as follows: 55.2mg/100mL.
This kind of edible fungus health-care drink has good antioxidant effect, and Scavenging activity and sample liquid extension rate are one
Determining tool in range is in dose-effect relationship;Wherein, DPPH, ABTS Scavenging activity are fine, and total antioxidant capacity, total reducing power are also suitable
It is good, but Hydroxyl radical-scavenging ability is general.
Effect of the hundred straw mushroom extracting solution of this experimental study for relief of constipation symptom, and its mechanism of action has been carried out tentatively
It inquires into, becomes the health food of relax bowel and defecation for hundred straw mushroom extracting solutions, provide fundamental basis.
1 materials and methods
1.1 materials and reagent
1.1.1 experimental animal
Healthy male mouse of kunming (18~22g of weight) Yanbian University's animal center.
1.1.2 drink dosage
Correlation test will be carried out after 4 times of sample concentration.
1.1.3 drug and reagent
The preparation of prepared Chinese ink: by gum arabic 100g, adding 800mL water, boils, the then work of addition 50g transparent to solution
Property carbon, then boil three times, water added to be settled to 1000mL, 4 DEG C of preservations;
Loperamide (national drug standard H10910085) Xian-Janssen Pharmaceutical Ltd.);
Beijing TongrenTang Co., Ltd Tongrentang Pharmaceutical Factory Maren wan (national drug standard Z11020160)).
1.2 test method
1.2.1 animal packet and administration
Male mice 60 are selected, 6 groups is randomly divided into, every group 10, there is blank group, Constipation Model group, positive controls
With the administration group of three various doses.Three administration groups distinguish the basic, normal, high dosage of orally administration (16.5,33,66mL/kg bw)
Hundred straw mushroom extracting solutions, blank group and model group give the physiological saline of 20mL/kg bw, and positive controls give 1.0g/kg
The Maren wan of bw, stomach-filling amount are 20mL/kg bw, once a day, continuous 8d, during which free diet and drinking-water.
1.2.2 mouse defecation is tested
After the last administration, each group mouse fasting 16h (drinking-water unlimited), model group, positive controls and three dosage are given
Medicine group mouse oral gives the Loperamide that dosage is 10mg/kg bw, and naive mice gives the physiological saline of same volume.It gives
After Loperamide 30min, blank group, model group, positive controls and three dosage administration group mouse give containing corresponding tested
The prepared Chinese ink of object, starts simultaneously at timing.Every mouse is individually raised, and free water is ingested, observe and record every mouse arrange for the first time it is black
Toilet takes time, defecation grain number and weight in 6h.
1.2.3 intestinal motility is tested
Select male mice 6, animal packet and the same 1.2.1 of administration mode.After the last administration, groups of animals fasting 16h (drink
Water is unlimited), the administration group mouse oral of model group, positive controls and three dosage gives the Lip river piperazine that dosage is l0mg/kg bw
Butylamine, naive mice give the distilled water of same volume.After 30min, the administration group of positive controls and high, medium and low dosage is small
Mouse gives the prepared Chinese ink containing corresponding tested material, and blank group and model group mouse give prepared Chinese ink.Give cervical dislocation after 25 min of prepared Chinese ink
Mouse is put to death, abdominal cavity is opened and separates mesenterium, clip upper end is placed on pallet from the intestinal tube of pylorus, lower end to ileocecus, gently
Small intestine is pulled into straight line to measure.Length of intestine is " total small intestinal length ", from pylorus to prepared Chinese ink forward position for " prepared Chinese ink promotes length
Degree ", is calculated according to the following formula ink progradation.
1.2.4 statistical analysis carries out variance analysis to indices initial data with SPSS17.0 software.
1.2.5 result judgement
Bowel relaxing functions determine: row's any one of fecal grains and 6h defecation weight result is positive in 6h, at the same for the first time defecation when
Between and any one of intestinal motility experiment result it is positive, can determine that this experimental result for the positive.
2 results and analysis
Influence of 2.1 tested materials to mouse weight
As shown in Table 2, each group mouse original body mass and each group mouse weight after hundred straw mushroom extracting solution 8d of orally administration and sky
White group is compared, difference that there are no significant (p > 0.05), and the normal increase of each tested material mouse weight can be illustrated without influence.
The original body mass of 2 mouse of table and experiment terminate preceding weight
* same column difference lowercase indicates that there are significant difference (P < 0.05) between different tests group.
Influence of the 2.2 hundred straw mushroom extracting solutions to mice with constipation defecation
As shown in Table 3, model group compared to the blank group, the time required to arranging melena for the first time, defecation grain number and 6h defecation in 6h
Weight has significant difference, shows that Constipated mice is successfully established (p < 0.05).Compared with model group, hundred straw mushroom extracting solutions
The fecal grains and excrement water content of middle and high dosage group mouse have significant difference (p < 0.05), and hundred straw mushroom extracting solutions are middle and high
(p < 0.05) is reduced with the increase of dosage the time required to the melena of row for the first time of dosage group mouse.The row of hundred straw mushroom extracting solutions
Just effect increases, and is in dose dependent.Defaecation by hundred straw mushroom extracting solutions of statistical analysis discovery high dose to mice with constipation
Effect is better than positive control medicine Maren wan (p < 0.05).
Influence of the 300 straw mushroom extracting solution of table to mice with constipation defecation
* there are significant difference (P < 0.05) between the different small and alphabetical expression different tests groups of same column.
Influence of the 2.3 hundred straw mushroom extracting solutions to mice with constipation small bowel peristalsis
As shown in Table 4, the ink progradation of model group and blank group significant difference (p < 0.05), show Constipation Model at
It is vertical.It is found through data statistic analysis, the ink progradation and constipation mould of basic, normal, high hundred straw mushroom extracting solution administration group mouse of dosage
Type group compares, and has significant difference (p < 0.05), shows that hundred straw mushroom extracting solutions have the function of that mouse small intestine is promoted to wriggle.
Influence of the 400 straw mushroom extracting solution of table to ink progradation
* there are significant difference (P < 0.05) between the different small and alphabetical expression different tests groups of same column.
Hundred straw mushroom extracting solutions contain polysaccharide, beta glucan, polyphenol, the effective components such as triterpene.Its effect for treating constipation can
Can with following factors in relation to: the hydrophilic radical that the polysaccharide in hundred straw mushroom extracting solutions contains, water imbibition is strong, and retentiveness is good, after water suction
Itself can be expanded into even ten times of youngster of original several times, can make excrement is soft to be easy to transport to anus from rectum, body is smoothly discharged
Outside;Simultaneously as faecal volume increases and intestinal wall is stimulated to wriggle, shorten the time that excrement excretes;There is drops in enteron aisle
The bacterium of non-starch polysaccharide is solved, polysaccharide cannot be directly absorbed by the body as the fermentation substrate of bacterium, and it is short to can produce effumability
Chain fatty acid reduces large intestine pH value, influences the metabolism of microorganism in enteron aisle, intestinal flora and quantity is made to change, and increases
Excrement amount;Other hundred straw mushrooms extracting solution can also protect mucous membrane of small intestine that atrophy does not occur, and reduce the possibility that constipation occurs.
It is mainly at present permeability for the treatment of constipation, bulk-forming and lubricity class cathartic.Permeability and lubricity medicine
Object can make excrement in watery or semi-liquid-like sometimes, increase the daily defecation frequency of patient, can reduce some patientss constipation symptom, but
Patient's anal skin can also be caused unclean, or even rotten to the corn.In addition, permeability and a large amount of bulk-forming drug can influence fat-soluble dimension
The absorption of raw element, long-term use can cause water and rock-soil coupling, can also cause intestinal smooth powerless, formed pharmacological dependence or
Incontinence of faces etc..According to the highest intake that human body extracts this, select the dosage of hundred straw mushroom extracting solutions for (16.5,33,
66)mL/kg bw.In research process, hundred straw mushroom extracting solutions do not impact the weight of mouse, and mouse is also not observed
There is symptom of diarrhea, show the mildness of its therapeutic effect, better relax bowel and defecation can be shown when dosage is lower than Maren wan
Effect, compared with its similar solvent borne cathartic, avoids bring side effect due to dosage is excessive.
In the present invention, inventor uses the place of production fine and soft for the Siberian birch of Russia, birch used in the present invention
Fine and soft extract has antitumaous effect, especially to kinds of tumor cells such as mastocarcinoma, lip cancer, gastric cancer, parotid gland cancer, lung cancer, skin
Cancer, the carcinoma of the rectum, Huo Jinsi lymph cancer have apparent inhibiting effect.
Birch young pilose antler extract of the invention prevents cancer metastasis, recurrence, enhances immunocompetence, promotes health;And it uses
Radiotherapy, chemotherapy in cooperation malignant tumor patient, enhance the tolerance of patient, mitigate toxic side effect;It can be with prophylactic treatment glycosuria
Disease;Birch young pilose antler extract anti-aging;Intracorporal free radical is removed, cell is protected, extends the division algebra of passage cell, is promoted thin
Born of the same parents' service life promotes metabolism, thus can effectively delay senescence, and long-term use can promote longevity;Birch young pilose antler extract effectively inhibits
Infective virus, can preventing cold;Birch young pilose antler extract prevents hypertension;Birch young pilose antler extract improves and prevents anaphylaxis skin
Matter;Birch young pilose antler extract has apparent prophylactic treatment to act on hepatitis, gastritis, duodenal ulcer, ephritis;And to vomiting, abdomen
It rushes down, gastrointestinal dysfunction has prophylactic treatment effect.
Birch young pilose antler is the fungi being grown on silver birch, is that fully stocked wood is presented to the rarity of the mankind.It is containing there are many to people
The beneficial biochemical composition of body, such as polysaccharide, inonotus obliquus, a variety of oxidation triterpenoids etc..
Superoxide dismutase (polyphenoils) contained by birch young pilose antler is 55 times of ganoderma lucidum, 23 times of Brazilian mushroom, monkey
25 times of head mushroom.
Functional beverage of the present invention, it is as the main component to originate from the Siberian birch young pilose antler extract of Russia,
Preparation is prepared according to the common method for preparing beverage.
The second aspect of the present invention also provides a kind of functional beverage, in addition to also containing birch young pilose antler containing of the present invention
Except extract, collybia albuminosa extract can also be contained.
The functional beverage that the second aspect of the present invention provides, it is various provided by birch young pilose antler extract in addition to having effects that
Outside, also have effects that various contained by collybia albuminosa extract.
It is recorded according to the materia medica such as Compendium of Material Medica and " new compilation of materia medica ", collybia albuminosa is with " beneficial stomach, clear mind control hemorrhoid and drop blood
, there are enriching the blood to restore normal menstruation, strengthening the spleen and stomach and other effects in the effects of rouge ", can be used for treating all diseases of blood under loss of appetite, endless diarrhea, hemorrhoid.It is existing
It is found in medical research, the effective ingredient in collybia albuminosa containing ergosterol substance and treatment diabetes has to blood glucose is reduced
Positive effect.And play the role of inhibiting human cancer cell's growth.
The effect of collybia albuminosa and effect are as follows: collybia albuminosa energy strengthening the spleen and stomach makes us appetite and increases;Collybia albuminosa includes
The multiple nutritional components such as calcium, phosphorus, iron, protein, be it is weak, after being ill with the delicacies of the elderly;Another feature of collybia albuminosa is to contain
Phosphorus amount is high, is the delicacies for needing to mend phosphorus personage;Often feeding collybia albuminosa can also improve immunity of organisms, resist cancer cell, reduce blood glucose;
Collybia albuminosa has enriching the blood to restore normal menstruation function, and women is well suited for.
It is first that the collybia albuminosa contains protein, fat necessary to human body, amino acid, various vitamins and potassium, calcium, magnesium etc.
Element contains the bioactive substances such as a large amount of polysaccharide, polyphenol.Collybia albuminosa is not only that body normal function provides safeguard, and improves machine
Body immunity, more prominent feature are that have powerful anti-radical action, and body metabolism can generate a large amount of free radical, and freely
The main reason for base can cause cancer, and more than the 100 kinds of diseases such as artery sclerosis and senile dementia are agings, collybia albuminosa is due to taste
The hat that road is delicious and nutrition is comprehensive and as mushroom.
The functional beverage that the third aspect of the present invention provides originates from the Siberian birch young pilose antler of Russia in addition to use and mentions
It takes except object, also using the collybia albuminosa extract for originating from Chinese yunnan, further contains Porcini extract.The bolete is excellent
Choosing is the bolete in Yunnan using the place of production.
There is a kind of work for making double-strand ribose sour component that there is antineoplaston viral hepatitis in bolete of the present invention
With, be the preferred food of tumour preoperative and postoperative, while there are also dissolving cholesterol, reduce blood pressure, blood lipid, resist serum cholesterol on
The effect rise, remove blood rubbish, increasing body immune function.Bolete is rare mushroom, and fragrance is unique, full of nutrition, is had
The function of prevent and cure diseases, keeping fit and healthy, has effects that strengthen immunity.Its water extract of bolete is to small white mouse sarcoma S-180
Growth have resistance inhibitor action, be 100% to the inhibiting rate of sarcoma S-180, the inhibiting rate to ehrlich ascites cancer is 90%, simultaneously also
There is resisiting influenza virus, prevent curing cold, is that China is exported to American-European famous edible mushroom.With heat-clearing solution tired, blood-nourishing and in,
Wind-evil dispelling and cold-evil expelling, relaxing muscles and tendons and blood, qi-restoratives are refreshed oneself and other effects, are one of the raw materials of Chinese patent drug " SHUJIN WAN ".Chinese medicine thinks to anaemia, body
Empty, dizziness, tinnitus are powerful.Gynaecology's good medicine can control women leukorrhea disease and infertility.Bolete contains 8 kinds of ammonia needed by human
Base acid, also containing alkaloids such as adenine, choline and putrescine.It is pharmaceutically acceptable, lumbocrural pain is treated, numb in every limb, tic of limbs is gone back
It can be used to control women's abnormal leucorrhea.It has heat-clearing solution tired, blood-nourishing and in, wind-evil dispelling and cold-evil expelling, relaxing tendons and activating collaterals, qi-restoratives the function such as refresh oneself
Effect.In addition, there are also resisiting influenza virus, the effect for preventing from catching a cold.It can be seen that cepe really multiple functional in mushroom, food in woods
The treasure of medicine dual-purpose.Frequent edible bolete can be remarkably reinforced immunity of organisms, improve body microcirculation.Bolete is rich in albumen
Matter, carbohydrate, multivitamin and minerals, his advantage are to significantly improve body immunity, nourshing blood and promoting blood circulation, qi-restoratives
Refresh oneself, is world-renowned high-quality edible mushroom.
In functional beverage of the invention, most preferably mentioned with birch young pilose antler extract, Termitomyces albuminosus with black skin extract, bolete
Taking object is raw material, and scientific formula is formed with modernization process Hydrolysis kinetics.
Functional beverage set of the invention birch young pilose antler, collybia albuminosa, bolete essence, for people improve inferior health and
Skin maintenance and cosmetic benefits provides best selection.Functional beverage of the invention is conducive to anti-oxidant, removing interior free yl, and enhancing is immune
Power, pre- anti-cancer.Make body rejuvenation, long-term drinking functional beverage of the invention can have a bath to your blood, can be effective
Hypoglycemic, reducing blood lipid and prevent hypertension.
Functional beverage of the invention is disorderly to endocrine disturbance, hepatitis, gastritis, duodenal ulcer, abdominal pain, gastrointestinal function
Disorderly also there are apparent opsonic action, long-term drinking functional beverage of the invention, the strong body of energy keeps the viscera function of human body prosperous
It contains, qi and blood enriches, resists and prevent germ from invading.Through the invention, it includes at least conditioning chronic disease, adjust endocrine, summary
Sleep quality, beautifying face and moistering lotion, improves immunity, is conditioning digestive system, blood pressure lowering, hypoglycemic, reducing blood lipid, anti-oxidant, clear at hyperthyroidism
Except free radical, eliminate bad breath constipation, pre- anti-cancer.
Claims (7)
1. a kind of functional beverage, wherein contain birch young pilose antler extract.
2. functional beverage described in claim 1, wherein the birch young pilose antler is to originate from the Siberian birch young pilose antler of Russia.
3. functional beverage according to claim 1 or 2, wherein also contain collybia albuminosa extract.
4. functional beverage according to claim 3, wherein the collybia albuminosa is the collybia albuminosa for originating from Yunnan.
5. functional beverage according to claim 3 or 4, wherein also contain Porcini extract.
6. functional beverage according to claim 5, wherein the bolete is the bolete for originating from Yunnan.
7. functional beverage according to any one of claim 1 to 6 is slept in conditioning chronic disease, adjusting endocrine, summary
Dormancy quality, beautifying face and moistering lotion, improves immunity, conditioning digestive system, blood pressure lowering, hypoglycemic, reducing blood lipid, anti-oxidant, removing at hyperthyroidism
Free radical eliminates bad breath, constipation, the application in pre- anti-cancer.
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