CN101530455A - Method for extracting antioxidant active substance in tasteless preserved soybean - Google Patents
Method for extracting antioxidant active substance in tasteless preserved soybean Download PDFInfo
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- CN101530455A CN101530455A CN200910119135A CN200910119135A CN101530455A CN 101530455 A CN101530455 A CN 101530455A CN 200910119135 A CN200910119135 A CN 200910119135A CN 200910119135 A CN200910119135 A CN 200910119135A CN 101530455 A CN101530455 A CN 101530455A
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- semen sojae
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Abstract
The invention relates to a method for extracting antioxidant active substance in tasteless preserved soybean, which includes steps of ethanol extraction, polyamide purifying, eluant condensation and drying for obtaining the extract with strong antioxidant activity, and the method belongs to natural product extraction field. The invention adopts tasteless preserved soybean as raw material to prepare strong antioxidant active substance, has simple process, low cost and simple device, is suitable for large-scale production, and establishes base for further exploiting antioxidant function foodstuff. The prepared tasteless preserved soybean antioxidant active substance product is suitable for industries such as medicine, health care product, foodstuff antioxidant and cosmetic.
Description
Technical field
The invention belongs to field of natural product extraction, particularly a kind of method of from Semen Sojae Preparatum, extracting anti-oxidation active substance.
Background technology
Semen Sojae Preparatum has another name called fragrant fermented soya beans, salted or other wise, light fermented soya beans, salted or other wise, big Semen Sojae Preparatum, be the mature seed of legume soybean (Glycine max (L.) Merr.) and the goods that Herba Artemisiae Annuae, Folium Mori steam net for catching birds or fish, ferment and process, rise in China, being the Chinese people's traditional food, also is one of first definite dietotherapeutic kind of China's Ministry of Public Health.As conventional Chinese medicine, " Chinese pharmacopoeia is all on the books to go through version.Because of the difference of concocting method, what have is cold in nature, the slight fever that has, and it has expelling pathogenic factors from muscles and delivers, declares strongly fragrant relieving restlessness, detoxifcation heat clearing away, rash antidotal effect, is applicable to the wind heat headache, vomiting uncomfortable in chest, diseases such as abundant expectoration fidgets due to deficiency.Semen Sojae Preparatum is rich in isoflavone and saponins compound.
The damage of radical pair cell and tissue is its morbific basis, and owing to human body is made up of the cell of various difference in functionality, thereby the different cells injury of radical pair can cause the surface to seem the disease that has no to correlate.As: free radical destroys cell membrane, causes cell membrane generation degeneration, makes cell not absorb nutrition from the outside, also can not drain intracellular metabolic waste, and has lost the defensive ability/resistance ability to antibacterial and virus; Free radical is attacked the gene that is duplicating, and causes gene mutation, brings out cancer and takes place, and free radical human activin immune system makes human body show anaphylaxis, or occurs as autoimmune diseases such as lupus erythematosus; Free radical acts on enzyme system in the human body, cause the release of Collagenase and hard elastics protease, these enzymes act on collagen protein and the hard elastics albumen in the skin and make this two kinds of albumen produce excessively crosslinked and degraded, and the result follows the string skin wrinkle and vesicle to occur; Similarly effect makes that capillary fragility increases in the body, and blood vessel is broken easily, and this can cause the generation of varicosis, edema etc. and vascular permeability rising diseases related; Free radical corrodes body tissue, but exciting human discharges various inflammatory factors, causes various non-mushroom inflammation; Free radical corrodes brain cell, makes the people get the disease of presenile dementia; Lipoprotein in the free-radical oxidation blood causes the deposition of cholesterol to blood vessel wall, causes heart disease and stroke; Free radical causes the degraded of membrana articulata and knuckle synovia, thereby causes arthritis; Free radical corrodes the eye lens tissue and causes cataract; Free radical corrodes the pancreas cell and causes diabetes.In a word, free radical can destroy collagen protein and other connective tissue, disturbs the important physical process, causes the cell DNA sudden change.Can reduce the probability of suffering from cardiovascular and cerebrovascular disease and eat antioxidant foods such as containing flavone.
Semen Sojae Preparatum is widely used, but the extraction and the antioxidation activity in vitro of its polyphenoils still lacked research.At present, do not see Semen Sojae Preparatum antioxidant Study on extraction report.
Summary of the invention
The purpose of this invention is to provide a kind of method of from Semen Sojae Preparatum, extracting anti-oxidation active substance.Adopt this method can effectively improve the antioxidation efficient of Semen Sojae Preparatum, reduce dose.This method has the extraction efficiency height, and technological process is simple, and equipment drops into little, and production cost is low, is easy to the advantage of industrialization.
The present invention is achieved through the following technical solutions:
A kind of method of from Semen Sojae Preparatum, extracting anti-oxidation active substance, the step of its extracting method is as follows:
(1) Semen Sojae Preparatum is pulverized, crossed 10～50 mesh sieves, make the Semen Sojae Preparatum coarse powder;
(2) the Semen Sojae Preparatum powder is put in the extraction vessel, added 10～30 times of concentration and be 50～95% alcoholic solution and extract, extract 50～85 ℃ of temperature, extraction time is 2～4h, repeat lixiviate 2～4 times, make that effective ingredient fully extracts in the Semen Sojae Preparatum, leach solution for later use;
(3) extracting solution is concentrated into 2～10 times of volumes of Semen Sojae Preparatum weight, goes up polyamide column then and carry out purification;
(4) the clear water eluting of 6～12 times of bed volumes of employing is removed water-solubility impurity;
(5) the alcoholic solution eluting of 60～95% concentration of 3～8 times of bed volumes of employing, eluent obtains anti-oxidation active substance through concentrated, lyophilization.
And the polyamide that purification adopts is 50～400 orders, and last sample loading mode adopts and dynamically goes up sample loading mode.
Advantage of the present invention and beneficial effect are:
It is feedstock production strong anti-oxidation active substance that the present invention adopts fermentation class Chinese medicine-Semen Sojae Preparatum commonly used, Semen Sojae Preparatum is huge in China's annual production, extracting raw material obtains easily, solvent is water and ethanol in the preparation process, avoided the use of poisonous organic solvent, the safety that can at utmost keep final products like this makes gained purification thing organic solvent-free residual.The all recyclable repeated use of used ethanol and polyamide, technological process and equipment simply are suitable for large-scale production, and lay a good foundation for further being developed as anti-oxidation functional food.Prepared tasteless preserved soybean antioxidant active substance product is applicable to industries such as medicine and health product, food antioxidant, cosmetics, and the extract residue can be used as feedstuff, fertilizer after processing, realizes comprehensive utilization.
The specific embodiment
The present invention is described in further detail by following examples, but the technology contents that present embodiment is narrated is illustrative, rather than determinate, should not limit to protection scope of the present invention with this.
The method step that extracts anti-oxidation active substance from Semen Sojae Preparatum involved in the present invention is as follows:
(1) Semen Sojae Preparatum is pulverized, crossed 50 mesh sieves, form the Semen Sojae Preparatum coarse powder;
(2) take by weighing 100 gram Semen Sojae Preparatum coarse powder, adding 1500mL concentration is 75% alcoholic solution in this Semen Sojae Preparatum powder, and 75 ℃ of extraction times are 3h, repeat lixiviate 3 times, and the extracting solution merging also is concentrated into 200mL.
(3) polyamide column carries out purification on the back, and polyamide adopts 100～200 orders, adopts and dynamically goes up sample loading mode; With UV (ultraviolet chromatograph) in 0.01mol/L NaOH aqueous solution in 273nm monitoring stream fluid, stop to go up sample when beginning to leak;
(3) the distilled water eluting of 10 times of bed volumes of employing is to remove water-solubility impurity;
(4) employing 5 times of bed volumes, concentration are 75% alcoholic solution eluting, and the eluent after the merging obtains anti-oxidation active substance through concentrated, lyophilization.
This antioxidation powder-product is mixed with 100ug/mL solution with methanol,, does test of DPPH free radical scavenging and the test of FRAP method oxidation resistance with Vc (vitamin c) and the positive contrast of BHT (2, the 4-di-tertiary butyl methyl phenol) with concentration.The results are shown in Table 1 and table 2 shown in.
Table 1 100ug/mL sample DPPH free radical scavenging activity
*Represent to exist between each sample significant difference (P＜0.01)
Table 2 100ug/mL sample F RAP method oxidation resistance
*Represent to exist between each sample significant difference (P＜0.01)
Claims (2)
1. a method of extracting anti-oxidation active substance from Semen Sojae Preparatum is characterized in that this method comprises the steps:
(1) Semen Sojae Preparatum is pulverized, crossed 10～50 mesh sieves, make the Semen Sojae Preparatum coarse powder;
(2) the Semen Sojae Preparatum powder is put in the extraction vessel, added 10～30 times of concentration and be 50～95% alcoholic solution and extract, extract 50～85 ℃ of temperature, extraction time is 2～4h, repeat lixiviate 2～4 times, make that effective ingredient fully extracts in the Semen Sojae Preparatum, leach solution for later use;
(3) extracting solution is concentrated into 2～10 times of volumes of Semen Sojae Preparatum weight, goes up polyamide column then and carry out purification;
(4) the clear water eluting of 6～12 times of bed volumes of employing is removed water-solubility impurity;
(5) the alcoholic solution eluting of 60～95% concentration of 3～8 times of bed volumes of employing, eluent obtains anti-oxidation active substance through concentrated, lyophilization.
2. a kind of method of from Semen Sojae Preparatum, extracting anti-oxidation active substance according to claim 1, it is characterized in that: the polyamide that purification adopts is 50～400 orders, last sample loading mode adopts and dynamically goes up sample.
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| CN200910119135A CN101530455A (en) | 2009-03-04 | 2009-03-04 | Method for extracting antioxidant active substance in tasteless preserved soybean |
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| CN200910119135A CN101530455A (en) | 2009-03-04 | 2009-03-04 | Method for extracting antioxidant active substance in tasteless preserved soybean |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793025A (en) * | 2012-08-08 | 2012-11-28 | 鲜活果汁工业（昆山）有限公司 | Method for using fermented soya bean grease as natural anti-oxidant |
| CN104770678A (en) * | 2015-04-01 | 2015-07-15 | 广西南宁桂知科技有限公司 | Fermented soya bean powder preparation method |
| CN106072574A (en) * | 2016-07-06 | 2016-11-09 | 广东科玮生物技术股份有限公司 | A kind of composite plant ferment and its preparation method and application |
| CN106113964A (en) * | 2016-06-30 | 2016-11-16 | 合肥环照高分子材料厂 | A kind of high antioxidant fountain solution compounding containing κ carrageenan Konjac glucomannan and preparation method thereof |
| CN115669863A (en) * | 2021-07-28 | 2023-02-03 | 四川多力贸易有限公司 | Process for producing fermented soybean product |
| CN116076716A (en) * | 2023-01-18 | 2023-05-09 | 中南林业科技大学 | Melatonin-enriched food extract and method of producing the same |
-
2009
- 2009-03-04 CN CN200910119135A patent/CN101530455A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793025A (en) * | 2012-08-08 | 2012-11-28 | 鲜活果汁工业（昆山）有限公司 | Method for using fermented soya bean grease as natural anti-oxidant |
| CN104770678A (en) * | 2015-04-01 | 2015-07-15 | 广西南宁桂知科技有限公司 | Fermented soya bean powder preparation method |
| CN106113964A (en) * | 2016-06-30 | 2016-11-16 | 合肥环照高分子材料厂 | A kind of high antioxidant fountain solution compounding containing κ carrageenan Konjac glucomannan and preparation method thereof |
| CN106072574A (en) * | 2016-07-06 | 2016-11-09 | 广东科玮生物技术股份有限公司 | A kind of composite plant ferment and its preparation method and application |
| CN115669863A (en) * | 2021-07-28 | 2023-02-03 | 四川多力贸易有限公司 | Process for producing fermented soybean product |
| CN116076716A (en) * | 2023-01-18 | 2023-05-09 | 中南林业科技大学 | Melatonin-enriched food extract and method of producing the same |
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Open date: 20090916 |