CN104856061A - Method for extracting anti-oxidative substance from aged white golden camellia tea - Google Patents
Method for extracting anti-oxidative substance from aged white golden camellia tea Download PDFInfo
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- CN104856061A CN104856061A CN201510353816.7A CN201510353816A CN104856061A CN 104856061 A CN104856061 A CN 104856061A CN 201510353816 A CN201510353816 A CN 201510353816A CN 104856061 A CN104856061 A CN 104856061A
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 16
- 239000000126 substance Substances 0.000 title abstract description 5
- 240000001548 Camellia japonica Species 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims abstract description 7
- 239000000047 product Substances 0.000 claims abstract description 7
- 239000011347 resin Substances 0.000 claims abstract description 6
- 229920005989 resin Polymers 0.000 claims abstract description 6
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 238000000746 purification Methods 0.000 claims abstract description 4
- 241001122767 Theaceae Species 0.000 claims description 42
- 239000000843 powder Substances 0.000 claims description 16
- 230000003064 anti-oxidating effect Effects 0.000 claims description 11
- 239000003963 antioxidant agent Substances 0.000 claims description 11
- 235000006708 antioxidants Nutrition 0.000 claims description 11
- 239000013543 active substance Substances 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 9
- 230000003292 diminished effect Effects 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 230000003647 oxidation Effects 0.000 claims description 6
- 238000007254 oxidation reaction Methods 0.000 claims description 6
- 230000002745 absorbent Effects 0.000 claims description 5
- 239000002250 absorbent Substances 0.000 claims description 5
- 239000006286 aqueous extract Substances 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 238000011068 loading method Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000004821 distillation Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 3
- 235000013376 functional food Nutrition 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 239000006227 byproduct Substances 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 235000019261 food antioxidant Nutrition 0.000 abstract description 2
- 229930014626 natural product Natural products 0.000 abstract description 2
- -1 that is Substances 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 241000209507 Camellia Species 0.000 abstract 4
- 235000019441 ethanol Nutrition 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 235000013616 tea Nutrition 0.000 description 34
- 230000000694 effects Effects 0.000 description 7
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 235000020334 white tea Nutrition 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 208000037386 Typhoid Diseases 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 201000008297 typhoid fever Diseases 0.000 description 2
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 1
- 241001205401 Aspergillus cristatus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- 241000607762 Shigella flexneri Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 150000002206 flavan-3-ols Chemical class 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 238000002376 fluorescence recovery after photobleaching Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003914 insulin secretion Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for extracting an anti-oxidative substance from aged white golden camellia tea, and belongs to the field of natural product extraction. The method comprises the following steps: sequential extraction with hot water and ethyl alcohol; and carrying out purification with macroporous adsorption resin AB-8, concentration and freeze-drying, so as to obtain the highly anti-oxidative extract. The method adopts the by-product, namely aged white golden camellia tea, of the aged white golden camellia tea industry as a raw material for preparation of the highly anti-oxidative substance, that is, waste is changed into valuable, so that the method is environmentally friendly; the method is relatively simple, does not require relatively large equipment investment, is very suitable for large scale industrial production, and can lay the foundation for development of series of functional aged white golden camellia tea foods. The prepared anti-oxidative substance product is suitable for the industries of food anti-oxidant, functional foods and medicine.
Description
Technical field
The invention belongs to field of natural product extraction, particularly a kind of method extracting anti-oxidation active substance from golden flower Lao Bai tea.
Background technology
Golden flower Lao Bai tea profile mostly is circular pie, its with Lao Bai tea for raw material is refining through material screening, decatize macerates heap, pressed sizing, the operation such as drying, finished product packing of growing dim processes.The good golden flower of quality old white tea cake complexion pool is dark brown, and fragrance is pure, and arohid flavour is strong, flavour alcohol and, soup look orange is bright." golden flower ", formal name used at school coronoid process falls apart capsule bacterium (Eurotium cristatum), be the key factor of achievement golden flower Lao Bai tea distinguishing character, the dense degree of " golden flower " is considered as the core index differentiating the old quality of white tea quality of golden flower, especially the important substance basis of Lao Bai tea health care effect.Lao Bai tea can Effective Regulation human body glycolipid metabolism, has the effect reducing cholesterol, triglyceride and blood sugar in blood of human body.In golden flower Lao Bai tea, the content of Tea Polyphenols is higher, and it is natural antioxidant, can play raising immunity and wait effect with protection is cardiovascular.Also containing the necessary organized enzyme of human body in white tea, can catabolism of fat be promoted, effectively control amount of insulin secretion, sugar unnecessary in decomposer inner blood, promote blood glucose balance.Existing scholar's research finds, the flavanols derivative, Flavonoids etc. of rich content is had mainly to there is flavone compound with the form of glucoside in golden flower Lao Bai tealeaves, its sugar combined is mainly glucose, multiple phenolic hydroxyl group is had in the molecular structure of these compounds, be natural antioxidant, there is important using value.There are some researches show in addition, golden flower Lao Bai tea to staphylococcus aureus, shigella dysenteriae, pneumococcus, typhoid bacillus, A type pays typhoid bacillus, Pseudomonas aeruginosa, expense Shigella flexneri etc. comparatively high inhibition effect and killing action.Golden flower Lao Bai tea decoction has inhibitory action to Fu Shi, sonne bacillus and Escherichia coli.Golden flower Lao Bai tealeaves has clearing heat and detoxicating, astringing to arrest bleeding, pain relieving of drying, stomach invigorating are regulated the flow of vital energy, hypoglycemic, desinsection, enhancing sperm motility, the effect such as antitumor.Found by large quantifier elimination, golden flower Lao Bai tea has very strong antioxidation activity, fully researchs and develops and utilize the oxidation-resistant active ingredient in golden flower Lao Bai tea, develops serial golden flower Lao Bai tea functional food lay the first stone and be significant next step.At present, there are no patent document report in this respect.
Summary of the invention
The invention discloses a kind of method extracting anti-oxidation active substance from golden flower Lao Bai tea, fill up at present there are no the blank of the oxidation-resistant active ingredient specialized in development and utilization golden flower Lao Bai tea, a kind of low cost is provided, is suitable for the method extracting anti-oxidation active substance from golden flower Lao Bai tea of industrialization development.
In order to reach above-mentioned object, the present invention adopts following concrete technical scheme: a kind of method extracting anti-oxidation active substance from golden flower Lao Bai tea, and the step of its extracting method is as follows:
(1) dried in shady and cool place by golden flower Lao Bai tea, micronizer pulverizes, crossing aperture is the sieve of 400 ~ 600 μm, forms golden flower Lao Bai tea leaf powder;
(2) take 6 ~ 10g golden flower Lao Bai tea leaf powder and be placed in 500 ml conical flasks, add the lixiviate in 75 ~ 95 DEG C of hot baths of 250 ~ 400mL distilled water, isothermal vibration lixiviate 15 ~ 30min, repeat lixiviate 2 ~ 3 times, filtration under diminished pressure while hot after lixiviate, filtrate to load in clean container to obtain golden flower Lao Bai tea leaf powder Aqueous extracts, and residue hot distilled water washs;
(3) take the golden flower Lao Bai tea leaf powder water extraction residue that 6 ~ 10g step (2) obtains and be placed in 500 ml conical flasks, adding 250 ~ 400mL concentration is that the ethanolic solution of 20 ~ 40% is at 45 ~ 65 DEG C, isothermal vibration lixiviate 30 ~ 40min, filtration under diminished pressure immediately after lixiviate, filtrate to load in clean container to obtain golden flower Lao Bai tea leaf powder alcohol extract;
(4) utilize the effective polyphenoils in macroporous absorbent resin separation golden flower Lao Bai tea leaf powder Aqueous extracts and alcohol extract, adopt the distilled water wash-out of 6 ~ 8 times of bed volumes, the high-efficiency antioxidant material in stepwise elution purification liquid;
(5) collected by stepwise elution liquid, detect anti-oxidant vigor, select the part reduced pressure distillation and concentration of high-efficiency antioxidant vigor, freeze drying, obtains oxidation resistant product.
As preferably, the model of step (4) described macroporous absorbent resin adopts AB-8, and sample loading mode adopts dynamic loading.
Compared with prior art, the present invention has following outstanding advantages and effect: (1) the present invention adopts the byproduct of golden flower Lao Bai tea industry---and golden flower Lao Bai tea is the active material that strong anti-oxidation prepared by raw material, turns waste into wealth, and is a kind of pollution-free industry; And technique is comparatively simple, comparatively need not invests by large equipment, be very suitable for large-scale industrialized production, develop serial golden flower Lao Bai tea functional food for next step and lay the first stone; (2) the anti-oxidation active substance product prepared by the present invention is applicable to food antioxidant, functional food and pharmaceuticals industry.
Detailed description of the invention
The present invention is described in further detail by following examples, but the present embodiment the technology contents that describes be illustrative, instead of determinate, protection scope of the present invention should do not limited to according to this.
The involved in the present invention method step extracting anti-oxidation active substance from golden flower Lao Bai tea is as follows:
(1) dried in shady and cool place by golden flower Lao Bai tea, micronizer pulverizes, crossing aperture is the sieve of 400 ~ 600 μm, forms golden flower Lao Bai tea leaf powder;
(2) take 10g golden flower Lao Bai tea leaf powder and be placed in 500 ml conical flasks, add the lixiviate in 95 DEG C of hot baths of 400mL distilled water, isothermal vibration lixiviate 30min, repeat lixiviate 2 times, filtration under diminished pressure while hot after lixiviate, filtrate loads clean containers for future use, and residue hot distilled water washs;
(3) take the golden flower Lao Bai tea leaf powder water extraction residue that 10g step (2) obtains and be placed in 500 ml conical flasks, adding 300mL concentration is that the ethanolic solution of 40% is at 45 DEG C, isothermal vibration lixiviate 40min, filtration under diminished pressure immediately after lixiviate, filtrate loads clean containers for future use;
(4) utilize the effective polyphenoils in AB-8 macroporous absorbent resin separation golden flower Lao Bai tea leaf powder Aqueous extracts and alcohol extract, adopt the distilled water wash-out of 6 ~ 8 times of bed volumes, the high-efficiency antioxidant material in segmentation purification liquid;
(5) collected by stepwise elution liquid, detect anti-oxidant vigor, select the part reduced pressure distillation and concentration of high-efficiency antioxidant vigor, freeze drying, obtains the Powdered oxidation resistant product of light/dark balance.
This light/dark balance powder-product methyl alcohol is mixed with 100 μ g/mL solution, with BHT and Vc of same concentration for positive control does DPPH radicals scavenging test and the test of FRAP method oxidation resistance.Its result is as follows:
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.
Claims (2)
1. from golden flower Lao Bai tea, extract a method for anti-oxidation active substance, it is characterized in that, the step of its extracting method is as follows:
(1) dried in shady and cool place by golden flower Lao Bai tea, micronizer pulverizes, crossing aperture is the sieve of 400 ~ 600 μm, forms golden flower Lao Bai tea leaf powder;
(2) take 6 ~ 10g golden flower Lao Bai tea leaf powder and be placed in 500 ml conical flasks, add the lixiviate in 75 ~ 95 DEG C of hot baths of 250 ~ 400mL distilled water, isothermal vibration lixiviate 15 ~ 30min, repeat lixiviate 2 ~ 3 times, filtration under diminished pressure while hot after lixiviate, filtrate to load in clean container to obtain golden flower Lao Bai tea leaf powder Aqueous extracts, and residue hot distilled water washs;
(3) take the golden flower Lao Bai tea leaf powder water extraction residue that 6 ~ 10g step (2) obtains and be placed in 500 ml conical flasks, adding 250 ~ 400mL concentration is that the ethanolic solution of 20 ~ 40% is at 45 ~ 65 DEG C, isothermal vibration lixiviate 30 ~ 40min, filtration under diminished pressure immediately after lixiviate, filtrate to load in clean container to obtain golden flower Lao Bai tea leaf powder alcohol extract;
(4) utilize the effective polyphenoils in macroporous absorbent resin separation golden flower Lao Bai tea leaf powder Aqueous extracts and alcohol extract, adopt the distilled water wash-out of 6 ~ 8 times of bed volumes, the high-efficiency antioxidant material in stepwise elution purification liquid;
(5) collected by stepwise elution liquid, detect anti-oxidant vigor, select the part reduced pressure distillation and concentration of high-efficiency antioxidant vigor, freeze drying, obtains oxidation resistant product.
2. a kind of method extracting anti-oxidation active substance from golden flower Lao Bai tea according to claim 1, it is characterized in that the model of step (4) described macroporous absorbent resin adopts AB-8, sample loading mode adopts dynamic loading.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510353816.7A CN104856061A (en) | 2015-06-24 | 2015-06-24 | Method for extracting anti-oxidative substance from aged white golden camellia tea |
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| CN201510353816.7A CN104856061A (en) | 2015-06-24 | 2015-06-24 | Method for extracting anti-oxidative substance from aged white golden camellia tea |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866833A (en) * | 2017-03-13 | 2017-06-20 | 广西南宁隆吉维特生物科技有限公司 | Camellia chrysantha polysaccharide with anti-stress effect and preparation method thereof |
| CN108703240A (en) * | 2018-05-02 | 2018-10-26 | 贵州芳瑞堂生物科技有限公司 | A kind of Ramulus et Folium Mussaendae Pubescentis extract of high polyphenol content |
| CN108719538A (en) * | 2018-05-02 | 2018-11-02 | 贵州芳瑞堂生物科技有限公司 | A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract |
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2015
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| JP2004305012A (en) * | 2003-04-02 | 2004-11-04 | Ito En Ltd | Method for producing decaffeinated natural plant extract |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106866833A (en) * | 2017-03-13 | 2017-06-20 | 广西南宁隆吉维特生物科技有限公司 | Camellia chrysantha polysaccharide with anti-stress effect and preparation method thereof |
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| CN108719538A (en) * | 2018-05-02 | 2018-11-02 | 贵州芳瑞堂生物科技有限公司 | A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract |
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