CN108719538A - A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract - Google Patents
A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract Download PDFInfo
- Publication number
- CN108719538A CN108719538A CN201810411231.XA CN201810411231A CN108719538A CN 108719538 A CN108719538 A CN 108719538A CN 201810411231 A CN201810411231 A CN 201810411231A CN 108719538 A CN108719538 A CN 108719538A
- Authority
- CN
- China
- Prior art keywords
- white tea
- extraction
- ramulus
- tea
- white
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/163—Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Dispersion Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a kind of preparation methods of Ramulus et Folium Mussaendae Pubescentis extract, including crushing, water carry, alcohol extracting, enzymolysis, ultrafiltration, concentration, dry obtained Ramulus et Folium Mussaendae Pubescentis extract.The present invention is by carrying water, alcohol extracting is combined with complex enzyme hydrolysis technology, can significantly improve the recovery rate of white tea active ingredient, is carried compared to simple water, the recovery rate of alcohol extracting can improve 10% or so.
Description
Technical field
The invention belongs to tea processing field, and in particular to a kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract.
Background technology
Tealeaves, with the development of science and technology, especially since the seventies in last century, Japan and the United States and China have carried out largely
Since research of the tealeaves to the healthcare function of human body, especially the later stage eighties, multi-disciplinary intersection and infiltration make tealeaves
Health-care efficacy research enters a leap, obtains many breakthrough achievements, and follow an ex vivo experiment-in vivo
The scale pattern of experiment-clinical trial-epidemiological study.
Chemical composition present in tealeaves, type are various, it has been found that more than 400 kinds, just make with apparent health care to human body
With being segmented into organic principle, including other combinations such as tea polyphenols, vitamin, amino acid, pigment and lipopolysaccharides, caffeine
Object；Another kind of is the inorganic constituents of tealeaves, i.e. mineral element, including various trace elements needed by human, is all deposited in tealeaves
?.The effect that Trace Elements in Tea generates health causes the attention of tea science man or even Medical scientists.
Tea polyphenols are the compounds with phenol chemical constitution for being present in tea, it has been found that have more than 30 kinds, wherein catechin
Account for about 70% or so of tea polyphenols total amount, be not only one of the main body of tealeaves green tea ingredient, and is why tealeaves has
There is the important component of healthcare function.Tealeaves contained vitamin type is quite a lot of, is more satisfactory as the supplement of human body day requirement
, especially vitamin C and content of vitamin E quite it is abundant, all there is anti-aging and oxidation resistant effect.
Free radical is the arch-criminal for threatening human health, and the catechu in tea polyphenols is known as the good work(for removing free radical
Effect, tea are big right plant products, are ideal health beverages.Further prove that tea is more according to clinic study in recent years
Catechin in phenol has anti-oxidant, radiation protection, lipid-loweringing, anticancer, anti-aging and other effects.
White tea belongs to slight fermented tea, is the special treasure in China's teas.Because its sample tea is mostly bud head, full slope pekoe,
As silver is gained the name like snow.The characteristics of white tea preparation method neither destructive enzyme activity, and do not promote oxidation, and keep milli fragrant aobvious
Existing, soup is delicious refreshing.Its property is refrigerant, have removing toxic substances, bring down a fever, fall fire the effect of, be summer good drink.White tea, except with the battalion in green tea
It forms point, its main feature is that the free amine group content in white tea is higher.Having tea can be such that the interferon of human blood immune cells secretes
Amount increases by 500, this is because there is the substance that one kind being called " theanine " in tealeaves, this theanine is decomposed into human liver
Ethamine, and ethamine can transfer the secretion of the humoral immunity cell promotion interferon of entitled " gamma-delta T shapes cell ", and
Interferon is that human body resists infection " chemical defence line ", therefore can improve the ability that human body resists extraneous infringement again.
With the quickening and variation of modern life rhythm, tradition " bubble drink " has been unable to meet multi-level consumption demand, in
It is that different-style and the fast and easily tea beverage of mouthfeel rich in functional component come into being.Although tea beverage currently on the market
Product is various, such as green tea beverage, ice black tea beverage, oolong tea beverage, jasmine tea beverage, but since white tea popularity is low
With white tea kind there are individual difference, if white tea soup look is shallow, tea perfume is light and slightly bitter, white tea beverage products pole in the market so far
It is few.
Although the technology of tea beverage processing in recent years makes much progress, many new and high technologies and equipment are answered
With, such as zymotechnic, membrane separation technique, microwave technology, aromatic substance recovery technology, freeze drying technology and aseptic filling technology.
However the research in terms of the domestic Ramulus et Folium Mussaendae Pubescentis extract to how to obtain high extraction is still relatively fewer.
Invention content
In order to solve the above technical problems, the present invention provides a kind of preparation methods of Ramulus et Folium Mussaendae Pubescentis extract.
The present invention is achieved by the following technical programs.
The present invention provides a kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, which is characterized in that includes the following steps：
(1) 10~30 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powders of 6~10g are weighed to be placed in 500ml conical flasks, 250~400mL distilled water is added at 75~95 DEG C
It is extracted in hot bath, isothermal vibration extracts 15~30min, repeats extraction 2~3 times, is filtered under diminished pressure while hot after extraction, filtrate
It is fitted into clean container and obtains white tea leaf powder Aqueous extracts, residue is washed with hot distilled water；
(3) the white tea leaf powder water that step (2) obtains is carried residue to be placed in 500ml conical flasks, it is dense that 250~400mL is added
For the ethanol solution that degree is 20~40% at 45~65 DEG C, isothermal vibration extracts 30~40min, is depressurized immediately after extraction
Filter, filtrate, which is fitted into clean container, obtains white tea leaf powder alcohol extract；
(4) the white tea leaf powder alcohol extracting residue that step (3) obtains is placed in 500ml conical flasks, is 1 by solid-liquid ratio：20
Ratio is added in deionized water, is added 0.1% acid protease and 0.02% pectase, adjust enzymolysis liquid pH to 5.0 into
Row extraction, extraction conditions are：Temperature 50 C, extraction time 60min, after enzymolysis enzyme deactivation live, be to extract through the filtering of 200 mesh
Liquid；
(5) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(6) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, in 7.8-8.2MPa, normal temperature condition after ultrafiltration
Under, millet paste is concentrated into the concentrate of 25-35% containing solid content or more with reverse osmosis membrane；
(7) concentrate is sprayed dry under the conditions of 250-260 DEG C of spray dryer inlet temperature, 110-120 DEG C of outlet temperature
It is dry, Ramulus et Folium Mussaendae Pubescentis extract is made.
Further, the white tea tealeaves in the step (1) be selected from white peony, silver tip pekoe, tribute eyebrow, Shou Meizhong it is arbitrary
One or more combinations.
Further, the white tea tealeaves grinding particle size in the step (1) is 15~25 mesh, more preferably 20 mesh.
Further, the enzyme activity of the acid protease in the step (4) is ten thousand u/g of 1-1.5, and the enzyme activity of pectase is
3000-5000u/g。
The beneficial effects of the present invention are：
The present invention is by carrying water, alcohol extracting is combined with complex enzyme hydrolysis technology, can significantly improve white tea active ingredient
Recovery rate is carried compared to simple water, the recovery rate of alcohol extracting can improve 10% or so.Meanwhile the present invention uses acid protease knot
The dissolution of the active ingredient in white tea cell can preferably be promoted by closing the technology of pectinase enzymatic hydrolysis, compared to using single enzyme or
Person uses the combination of cellulase combining pectin enzyme, can improve recovery rate 3-5% of white tea active ingredient or so.Compared to existing
Technology, present invention obtains significant progress effect, also obtained in terms of the selection of compound enzyme unexpected research at
Fruit.
Specific implementation mode
Technical scheme of the present invention is described further below by specific embodiment, but following specific examples does not have to
In the limitation present invention.It is all within spirit of that invention and principle, any modification, equivalent replacement, improvement and so on should all include
Within protection scope of the present invention.
Embodiment 1
A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, includes the following steps：
(1) 20 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powders of 8g are weighed to be placed in 500ml conical flasks, 350mL distilled water is added and is extracted in 85 DEG C of hot baths,
Isothermal vibration extracts 20min, repeats extraction 3 times, is filtered under diminished pressure while hot after extraction, and filtrate is fitted into clean container and obtains in vain
Tea leaf powder Aqueous extracts, residue are washed with hot distilled water；
(3) the white tea leaf powder water that step (2) obtains is carried residue to be placed in 500ml conical flasks, it is a concentration of that 350mL is added
For 30% ethanol solution at 55 DEG C, isothermal vibration extracts 35min, is filtered under diminished pressure immediately after extraction, and filtrate is packed into clean
White tea leaf powder alcohol extract is obtained in container；
(4) the white tea leaf powder alcohol extracting residue that step (3) obtains is placed in 500ml conical flasks, is 1 by solid-liquid ratio：20
Ratio is added in deionized water, is added 0.1% acid protease and 0.02% pectase, adjust enzymolysis liquid pH to 5.0 into
Row extraction, extraction conditions are：Temperature 50 C, extraction time 60min, after enzymolysis enzyme deactivation live, be to extract through the filtering of 200 mesh
Liquid；
(5) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(6) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, after ultrafiltration under 8.0MPa, normal temperature condition, used
Millet paste is concentrated into the concentrate containing 30% or more solid content by reverse osmosis membrane；
(7) concentrate is spray-dried under the conditions of 250 DEG C of spray dryer inlet temperature, 110 DEG C of outlet temperature, is made white
Tea extraction.
White tea tealeaves in the step (1) is silver tip pekoe.
The enzyme activity of acid protease in the step (4) is 10,000 u/g, and the enzyme activity of pectase is 3000u/g.
Embodiment 2
A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, includes the following steps：
(1) 10 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powders of 6g are weighed to be placed in 500ml conical flasks, 250mL distilled water is added and is extracted in 75 DEG C of hot baths,
Isothermal vibration extracts 15min, repeats extraction 2 times, is filtered under diminished pressure while hot after extraction, and filtrate is fitted into clean container and obtains in vain
Tea leaf powder Aqueous extracts, residue are washed with hot distilled water；
(3) the white tea leaf powder water that step (2) obtains is carried residue to be placed in 500ml conical flasks, it is a concentration of that 250mL is added
For 20% ethanol solution at 45 DEG C, isothermal vibration extracts 30min, is filtered under diminished pressure immediately after extraction, and filtrate is packed into clean
White tea leaf powder alcohol extract is obtained in container；
(4) the white tea leaf powder alcohol extracting residue that step (3) obtains is placed in 500ml conical flasks, is 1 by solid-liquid ratio：20
Ratio is added in deionized water, is added 0.1% acid protease and 0.02% pectase, adjust enzymolysis liquid pH to 5.0 into
Row extraction, extraction conditions are：Temperature 50 C, extraction time 60min, after enzymolysis enzyme deactivation live, be to extract through the filtering of 200 mesh
Liquid；
(5) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(6) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, after ultrafiltration under 7.8MPa, normal temperature condition, used
Millet paste is concentrated into the concentrate containing 25% or more solid content by reverse osmosis membrane；
(7) concentrate is spray-dried under the conditions of 260 DEG C of spray dryer inlet temperature, 120 DEG C of outlet temperature, is made white
Tea extraction.
White tea tealeaves in the step (1) is white peony.
The enzyme activity of acid protease in the step (4) is 1.5 ten thousand u/g, and the enzyme activity of pectase is 5000u/g.
Embodiment 3
A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, includes the following steps：
(1) 30 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powders of 10g are weighed to be placed in 500ml conical flasks, 400mL distilled water is added and is soaked in 95 DEG C of hot baths
It carries, isothermal vibration extracts 30min, repeats extraction 3 times, is filtered under diminished pressure while hot after extraction, filtrate is fitted into clean container
White tea leaf powder Aqueous extracts are obtained, residue is washed with hot distilled water；
(3) the white tea leaf powder water that step (2) obtains is carried residue to be placed in 500ml conical flasks, it is a concentration of that 400mL is added
For 40% ethanol solution at 65 DEG C, isothermal vibration extracts 40min, is filtered under diminished pressure immediately after extraction, and filtrate is packed into clean
White tea leaf powder alcohol extract is obtained in container；
(4) the white tea leaf powder alcohol extracting residue that step (3) obtains is placed in 500ml conical flasks, is 1 by solid-liquid ratio：20
Ratio is added in deionized water, is added 0.1% acid protease and 0.02% pectase, adjust enzymolysis liquid pH to 5.0 into
Row extraction, extraction conditions are：Temperature 50 C, extraction time 60min, after enzymolysis enzyme deactivation live, be to extract through the filtering of 200 mesh
Liquid；
(5) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(6) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, after ultrafiltration under 8.2MPa, normal temperature condition, used
Millet paste is concentrated into the concentrate containing 35% or more solid content by reverse osmosis membrane；
(7) concentrate is spray-dried under the conditions of 260 DEG C of spray dryer inlet temperature, 120 DEG C of outlet temperature, is made white
Tea extraction.
White tea tealeaves in the step (1) is tribute eyebrow.
The enzyme activity of acid protease in the step (4) is 1.2 ten thousand u/g, and the enzyme activity of pectase is 4000u/g.
Embodiment 4
A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, includes the following steps：
(1) 20 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powders of 8g are weighed to be placed in 500ml conical flasks, 350mL distilled water is added and is extracted in 85 DEG C of hot baths,
Isothermal vibration extracts 20min, repeats extraction 3 times, is filtered under diminished pressure while hot after extraction, and filtrate is fitted into clean container and obtains in vain
Tea leaf powder Aqueous extracts, residue are washed with hot distilled water；
(3) the white tea leaf powder water that step (2) obtains is carried residue to be placed in 500ml conical flasks, it is a concentration of that 350mL is added
For 30% ethanol solution at 55 DEG C, isothermal vibration extracts 35min, is filtered under diminished pressure immediately after extraction, and filtrate is packed into clean
White tea leaf powder alcohol extract is obtained in container；
(4) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(5) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, after ultrafiltration under 8.0MPa, normal temperature condition, used
Millet paste is concentrated into the concentrate containing 30% or more solid content by reverse osmosis membrane；
(6) concentrate is spray-dried under the conditions of 250 DEG C of spray dryer inlet temperature, 110 DEG C of outlet temperature, is made white
Tea extraction.
White tea tealeaves in the step (1) is silver tip pekoe.
Embodiment 5
A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, includes the following steps：
(1) 20 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powder that step (1) obtains is placed in 500ml conical flasks, is 1 by solid-liquid ratio：20 ratio is added
In deionized water, 0.1% acid protease and 0.02% pectase is added, adjusts enzymolysis liquid pH to 5.0 and extracts, carry
The condition is taken to be：Temperature 50 C, extraction time 60min, after enzymolysis enzyme deactivation live, be extracting solution through the filtering of 200 mesh；
(3) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(4) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, after ultrafiltration under 8.0MPa, normal temperature condition, used
Millet paste is concentrated into the concentrate containing 30% or more solid content by reverse osmosis membrane；
(5) concentrate is spray-dried under the conditions of 250 DEG C of spray dryer inlet temperature, 110 DEG C of outlet temperature, is made white
Tea extraction.
White tea tealeaves in the step (1) is silver tip pekoe.
The enzyme activity of acid protease in the step (2) is 10,000 u/g, and the enzyme activity of pectase is 3000u/g.
Embodiment 6
A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, includes the following steps：
(1) 20 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powders of 8g are weighed to be placed in 500ml conical flasks, 350mL distilled water is added and is extracted in 85 DEG C of hot baths,
Isothermal vibration extracts 20min, repeats extraction 3 times, is filtered under diminished pressure while hot after extraction, and filtrate is fitted into clean container and obtains in vain
Tea leaf powder Aqueous extracts, residue are washed with hot distilled water；
(3) the white tea leaf powder water that step (2) obtains is carried residue to be placed in 500ml conical flasks, it is a concentration of that 350mL is added
For 30% ethanol solution at 55 DEG C, isothermal vibration extracts 35min, is filtered under diminished pressure immediately after extraction, and filtrate is packed into clean
White tea leaf powder alcohol extract is obtained in container；
(4) the white tea leaf powder alcohol extracting residue that step (3) obtains is placed in 500ml conical flasks, is 1 by solid-liquid ratio：20
Ratio is added in deionized water, and 0.1% acid protease is added, and adjusts enzymolysis liquid pH to 5.0 and extracts, extraction conditions
For：Temperature 50 C, extraction time 60min, after enzymolysis enzyme deactivation live, be extracting solution through the filtering of 200 mesh；
(5) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(6) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, after ultrafiltration under 8.0MPa, normal temperature condition, used
Millet paste is concentrated into the concentrate containing 30% or more solid content by reverse osmosis membrane；
(7) concentrate is spray-dried under the conditions of 250 DEG C of spray dryer inlet temperature, 110 DEG C of outlet temperature, is made white
Tea extraction.
White tea tealeaves in the step (1) is silver tip pekoe.
The enzyme activity of acid protease in the step (4) is 10,000 u/g.
Embodiment 7
A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, includes the following steps：
(1) 20 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powders of 8g are weighed to be placed in 500ml conical flasks, 350mL distilled water is added and is extracted in 85 DEG C of hot baths,
Isothermal vibration extracts 20min, repeats extraction 3 times, is filtered under diminished pressure while hot after extraction, and filtrate is fitted into clean container and obtains in vain
Tea leaf powder Aqueous extracts, residue are washed with hot distilled water；
(3) the white tea leaf powder water that step (2) obtains is carried residue to be placed in 500ml conical flasks, it is a concentration of that 350mL is added
For 30% ethanol solution at 55 DEG C, isothermal vibration extracts 35min, is filtered under diminished pressure immediately after extraction, and filtrate is packed into clean
White tea leaf powder alcohol extract is obtained in container；
(4) the white tea leaf powder alcohol extracting residue that step (3) obtains is placed in 500ml conical flasks, is 1 by solid-liquid ratio：20
Ratio is added in deionized water, and 0.1% cellulase and 0.02% pectase is added, and adjusts enzymolysis liquid pH to 5.0 and carries out
Extraction, extraction conditions are：Temperature 50 C, extraction time 60min, after enzymolysis enzyme deactivation live, be to extract through the filtering of 200 mesh
Liquid；
(5) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(6) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, after ultrafiltration under 8.0MPa, normal temperature condition, used
Millet paste is concentrated into the concentrate containing 30% or more solid content by reverse osmosis membrane；
(7) concentrate is spray-dried under the conditions of 250 DEG C of spray dryer inlet temperature, 110 DEG C of outlet temperature, is made white
Tea extraction.
White tea tealeaves in the step (1) is silver tip pekoe.
The enzyme activity of cellulase in the step (4) is 10,000 u/g, and the enzyme activity of pectase is 3000u/g.
Embodiment 8
Using tea polyphenols detection technique general in the prior art, in the Ramulus et Folium Mussaendae Pubescentis extract obtained to embodiment 1-7
Polyphenol content is detected, and is detected to the polyphenol content in white tea tealeaves used in embodiment 1-7, each to calculate
The recovery rate of active ingredient in embodiment white tea tealeaves (recovery rate is in terms of tea polyphenols).It is calculated according to the following formula：Recovery rate=carry
Take in liquid tea polyphenols amount х 100% in tea polyphenols amount/extraction raw material.
The result of calculation of each embodiment tea polyphenol extract rate is referring to the following table 1.
The comparison of effective component extraction rate in 1 Ramulus et Folium Mussaendae Pubescentis extract of table
| Group | Recovery rate (%) |
| Embodiment 1 | 74.82 |
| Embodiment 2 | 75.27 |
| Embodiment 3 | 75.15 |
| Embodiment 4 | 65.77 |
| Embodiment 5 | 69.25 |
| Embodiment 6 | 70.02 |
| Embodiment 7 | 71.36 |
By the result in upper table it can be found that the embodiment of the present invention 1-3 by the way that water is carried, alcohol extracting and complex enzyme hydrolysis technology
It is combined, the recovery rate of white tea active ingredient can be significantly improved, carried compared to simple water in embodiment 4, the recovery rate energy of alcohol extracting
Enough improve nearly 10%.Meanwhile the embodiment of the present invention 1-3 can be preferable using the technology of acid protease combining pectin enzyme enzymolysis
Ground promotes the dissolution of the active ingredient in white tea cell, compared to using single enzyme or using cellulase combining pectin enzyme
Embodiment 5-7 can also improve recovery rate 3-5% of white tea active ingredient or so.Therefore, technical scheme of the present invention is compared to existing
For having technology, obtain significant progress effect, also obtained in terms of the selection of compound enzyme unexpected research at
Fruit.
The concrete scheme and main feature of the present invention has been shown and described above, and to a certain extent by test data
Embody advantages of the present invention.It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, above-mentioned reality
The concrete scheme for only illustrating the present invention described in example and specification is applied, in the premise for not departing from spirit and scope of the invention
Under, various changes and improvements may be made to the invention, and these changes and improvements all fall within the protetion scope of the claimed invention.This hair
Bright claimed range is defined by the appending claims and its equivalent thereof.
Claims (4)
1. a kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract, which is characterized in that include the following steps：
(1) 10~30 mesh powders will be ground into after white tea tea-drying；
(2) the white tea leaf powders of 6~10g are weighed to be placed in 500ml conical flasks, 250~400mL distilled water is added in 75~95 DEG C of hot water
It is extracted in bath, isothermal vibration extracts 15~30min, repeats extraction 2~3 times, is filtered under diminished pressure while hot after extraction, and filtrate is packed into
White tea leaf powder Aqueous extracts are obtained in clean container, residue is washed with hot distilled water；
(3) the white tea leaf powder water that step (2) obtains is carried residue to be placed in 500ml conical flasks, it is a concentration of that 250~400mL is added
For 20~40% ethanol solution at 45~65 DEG C, isothermal vibration extracts 30~40min, is filtered under diminished pressure immediately after extraction, filters
Liquid, which is fitted into clean container, obtains white tea leaf powder alcohol extract；
(4) the white tea leaf powder alcohol extracting residue that step (3) obtains is placed in 500ml conical flasks, is 1 by solid-liquid ratio：20 ratio
It is added in deionized water, 0.1% acid protease and 0.02% pectase is added, adjust enzymolysis liquid pH to 5.0 and carried
It takes, extraction conditions are：Temperature 50 C, extraction time 60min, after enzymolysis enzyme deactivation live, be extracting solution through the filtering of 200 mesh；
(5) extracting solution of above steps is merged, storage tank is sent into after screen filtration；
(6) ultrafiltration is carried out with the ultrafiltration membrane of 60000-80000 molecular weight, after ultrafiltration under 7.8-8.2MPa, normal temperature condition, used
Millet paste is concentrated into the concentrate of 25-35% containing solid content or more by reverse osmosis membrane；
(7) concentrate is spray-dried under the conditions of 250-260 DEG C of spray dryer inlet temperature, 110-120 DEG C of outlet temperature, system
Obtain Ramulus et Folium Mussaendae Pubescentis extract.
2. the preparation method of Ramulus et Folium Mussaendae Pubescentis extract as described in claim 1, which is characterized in that the white tea tea in the step (1)
Leaf is selected from the combination of any one or more of white peony, silver tip pekoe, tribute eyebrow, Shou Meizhong.
3. the preparation method of Ramulus et Folium Mussaendae Pubescentis extract as described in claim 1, which is characterized in that the white tea tea in the step (1)
Leaf grinding particle size is 15~25 mesh.
4. the preparation method of Ramulus et Folium Mussaendae Pubescentis extract as described in claim 1, which is characterized in that the acid egg in the step (4)
The enzyme activity of white enzyme is ten thousand u/g of 1-1.5, and the enzyme activity of pectase is 3000-5000u/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810411231.XA CN108719538A (en) | 2018-05-02 | 2018-05-02 | A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810411231.XA CN108719538A (en) | 2018-05-02 | 2018-05-02 | A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108719538A true CN108719538A (en) | 2018-11-02 |
Family
ID=63939537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810411231.XA Pending CN108719538A (en) | 2018-05-02 | 2018-05-02 | A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108719538A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110353062A (en) * | 2019-08-09 | 2019-10-22 | 河北华朗生物科技有限公司 | A kind of formula and its preparation process of cold extraction Ramulus et Folium Mussaendae Pubescentis extract solid beverage |
| CN111729016A (en) * | 2020-03-26 | 2020-10-02 | 华东师范大学 | White tea extract, preparation method and administration method thereof and application of white tea extract in olfactory injury repair |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005071A (en) * | 2012-12-19 | 2013-04-03 | 福建绿叶茶业股份有限公司 | Solid-state tea containing white tea extract |
| CN103655928A (en) * | 2013-11-26 | 2014-03-26 | 北京联合大学生物化学工程学院 | Complex enzyme method for extracting tea polyphenol from tea leaves |
| CN104856061A (en) * | 2015-06-24 | 2015-08-26 | 福建省福鼎市名山茶叶有限公司 | Method for extracting anti-oxidative substance from aged white golden camellia tea |
| CN106726898A (en) * | 2016-12-07 | 2017-05-31 | 杭州银茗生物技术有限公司 | A kind of Ramulus et Folium Mussaendae Pubescentis extract and preparation method and application |
-
2018
- 2018-05-02 CN CN201810411231.XA patent/CN108719538A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005071A (en) * | 2012-12-19 | 2013-04-03 | 福建绿叶茶业股份有限公司 | Solid-state tea containing white tea extract |
| CN103655928A (en) * | 2013-11-26 | 2014-03-26 | 北京联合大学生物化学工程学院 | Complex enzyme method for extracting tea polyphenol from tea leaves |
| CN104856061A (en) * | 2015-06-24 | 2015-08-26 | 福建省福鼎市名山茶叶有限公司 | Method for extracting anti-oxidative substance from aged white golden camellia tea |
| CN106726898A (en) * | 2016-12-07 | 2017-05-31 | 杭州银茗生物技术有限公司 | A kind of Ramulus et Folium Mussaendae Pubescentis extract and preparation method and application |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110353062A (en) * | 2019-08-09 | 2019-10-22 | 河北华朗生物科技有限公司 | A kind of formula and its preparation process of cold extraction Ramulus et Folium Mussaendae Pubescentis extract solid beverage |
| CN111729016A (en) * | 2020-03-26 | 2020-10-02 | 华东师范大学 | White tea extract, preparation method and administration method thereof and application of white tea extract in olfactory injury repair |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103283878B (en) | Processing technology of white tea drinks | |
| CN102030834B (en) | Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide | |
| CN108703240A (en) | A kind of Ramulus et Folium Mussaendae Pubescentis extract of high polyphenol content | |
| CN103689158B (en) | Preparation method of chrysanthemum morifolium extract | |
| CN102919422B (en) | Process for extracting herbal tea concentrate | |
| CN104277915A (en) | Method for extracting peony essential oil from peony | |
| CN103342668B (en) | A kind of simple and easy method extracting natural taurine from abalone internal organ | |
| CN108719538A (en) | A kind of preparation method of Ramulus et Folium Mussaendae Pubescentis extract | |
| CN103535470B (en) | The production technology of a kind of GABA tealeaves or tea powder | |
| CN102132931B (en) | Method for preparing chrysanthemum instant powder or instant crystal | |
| CN105733809A (en) | Method and equipment for producing jasmine hydrolat through low temperature distillation | |
| CN102206550B (en) | Method for preparing osmanthus concrete and osmanthus total flavonoid through enzyme hydrolysis technology | |
| CN105475567A (en) | Dendrobe and cyclocarya paliurus extract composite tea and preparation method of dendrobe and cyclocarya paliurus extract composite tea | |
| CN103951713A (en) | Comprehensive utilization method for improving value of cucumis sativus seed medicinal materials | |
| CN106581108B (en) | Method for removing residual pesticide propamocarb in ginseng extract | |
| CN109170885A (en) | A method of extracting ginseng effective component | |
| CN104982597A (en) | Multi-functional composite instant tea, as well as preparation method and use thereof | |
| CN102805171B (en) | Process for utilizing tea comprehensively | |
| CN104586910B (en) | Coreopsis tinctoria extract and preparation method thereof | |
| CN103749766A (en) | Lung-heat-clearing and throat-moistening tea drink and preparation method thereof | |
| CN105410281A (en) | Preparation method of composite tea composed of extracts of cyclocarya paliurus and gingko | |
| CN105153320A (en) | Laminarin extraction method | |
| CN103931814A (en) | Black tea capable of supporting healthy energy and conserving strength and preparation method thereof | |
| CN110637902A (en) | Black tea capable of strengthening vitality and nourishing qi and preparation method thereof | |
| CN105287648B (en) | Sea is delivered vegetables the extraction preparation method and applications of active constituent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181102 |
|
| RJ01 | Rejection of invention patent application after publication |