CN106866833A - Camellia chrysantha polysaccharide with anti-stress effect and preparation method thereof - Google Patents
Camellia chrysantha polysaccharide with anti-stress effect and preparation method thereof Download PDFInfo
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- CN106866833A CN106866833A CN201710151756.XA CN201710151756A CN106866833A CN 106866833 A CN106866833 A CN 106866833A CN 201710151756 A CN201710151756 A CN 201710151756A CN 106866833 A CN106866833 A CN 106866833A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
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Abstract
The invention belongs to biomedicine technical field, a kind of particularly purposes of natural product active ingredient and preparation method thereof.The technical problems to be solved by the invention, are to provide a kind of camellia chrysantha polysaccharide extract with anti-stress effect and preparation method thereof.Technical scheme is as follows：The withe or leaf of Camellia nitidissima are taken, meal is broken into, after 10 times of 85% ethanol room temperature diacolation 24h of weight, percolate is released, is repeated 3 times, merge percolate, be concentrated under reduced pressure to give alcohol extract, can separately done him and use.The dregs of a decoction add 10 times of water of weight, are heated to reflux 2h, filter, and are repeated 3 times, merging filtrate, are concentrated under reduced pressure to after a small amount of volume, add 95% ethanol, the ethanol content of solution is reached 80%, stand 24h, 3000r/min centrifugation 10min, take out precipitation, as Thick many candies.The present invention provides a kind of possible medicine with resisting stress effect, and preparation method is simple and easy to apply, with low cost, is conducive to deeply developing for resource.
Description
Technical field
The invention belongs to biomedicine technical field, the particularly a kind of purposes of natural product active ingredient and its preparation
Method.
Background technology
Camellia nitidissima Camellia nitidissima be Theaceae Theaceae Camellias Camellia evergreen shrubs or
Dungarunga, its flower and leaf are Zhuang nationality in Guangxi traditional medication among the people, according to《Guangxi Chinese medicine standard》,《Modern Compendium of Materia Medica》Deng note
Carry, can be used to prevent and treat sphagitis, ephritis, dysentery, tumour is had blood in stool, the illness such as hypertension and irregular menstruation.It has been included within 2010
New resource food (Ministry of Public Health announce 2010 No. 9).Modern pharmacology experiment proves Camellia nitidissima in antibacterial, anti-oxidant, antiallergy
Etc. aspect show potential medical value.Camellia nitidissima contains various chemical compositions, including flavonoids, Tea Polyphenols, tea polysaccharide, soap
Glycoside, several amino acids and various micro- and vitamins beneficial to human body etc..But for a long time to Camellia nitidissima
Exploitation are limited to very much, are exactly mostly to use colored as tea beverage or art, the exploitation of the other parts such as stem, leaf
Report seldom, cause whole development not enough, the wasting of resources.
Stress (stress) whole body that to be body occur when various internal and external environment factors and society, psychological factor stimulate
The non-specific adaptive response of property or locality, is also called stress reaction.It is the important composition portion of organism adaptation, protection mechanism
Point, be conducive to being maintained in the environment for changing the homeostasis of body, strengthen the adaptability of body.But when body is chronically at
During stress situation, its neuroendocrine function can be significantly affected, and adaptive faculty and resistance of body etc. can be caused to decline, and be
The pathogenetic basis of the various diseases of human body.In recent years, with the quickening and life and the deterioration of natural environment of people's rhythm of life,
The important deterrent of human health stress have been turned into.Therefore, slow down stress generation or accelerate it to eliminate to maintaining the mankind
Health has great significance.
Anti-stress medicine refer to have alleviate, prevent and treat by stressor cause stress syndrome medicine, can be divided into stress
Prophylactic, promote to adapt to agent or stress alleviant.Research and application show that the polysaccharide compound such as lentinan, GL-B has
There are obvious resisting stress and immunoregulation effect, the immune system of body can be activated, improve its immunologic function.
The content of the invention
The technical problems to be solved by the invention, be to provide a kind of camellia chrysantha polysaccharide extract with anti-stress effect and
Its preparation method.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
Take the withe or leaf of Camellia nitidissima, break into meal, with 10 times of 85% ethanol room temperature diacolation 24h of weight after, releasing is oozed
Filter liquid, be repeated 3 times, merge percolate, be concentrated under reduced pressure to give alcohol extract, can separately do his and use.
The dregs of a decoction add 10 times of water of weight, are heated to reflux 2h, filter, and are repeated 3 times, merging filtrate, are concentrated under reduced pressure to a small amount of
After volume, 95% ethanol is added, the ethanol content of solution is reached 80%, stand 24h, 3000r/min centrifugation 10min, taken out
Precipitation, as Thick many candies.
Benefit effect of the invention is as follows：
1. a kind of possible medicine with resisting stress effect is provided, because main component is plant polyose, is not had
The apparent side effect and cumulative toxicity of Western medicine；
2. preparation method is simple and easy to apply, with low cost, uses the withe of Camellia nitidissima usually essentially as discarded object
Or leaf, be conducive to deeply developing for resource.
Specific embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1
Take totally 2 kilograms of the withe and leaf of Camellia nitidissima, break into meal, with 10 times of 85% ethanol room temperature diacolation 24h of weight after,
Percolate is released, is repeated 3 times, merge percolate, be concentrated under reduced pressure to give alcohol extract, can separately done him and use.
The dregs of a decoction add 10 times of water of weight, are heated to reflux 2h, filter, and are repeated 3 times, merging filtrate, are concentrated under reduced pressure to a small amount of
After volume, 95% ethanol is added, the ethanol content of solution is reached 80%, stand 24h, 3000r/min centrifugation 10min, taken out
Precipitation, as Thick many candies A obtains 60.4g after freezing.
Embodiment 2
Take 2 kilograms of the withe of Camellia nitidissima, break into meal, with 10 times of 85% ethanol room temperature diacolation 24h of weight after, releasing is oozed
Filter liquid, be repeated 3 times, merge percolate, be concentrated under reduced pressure to give alcohol extract, can separately do his and use.
The dregs of a decoction add 10 times of water of weight, are heated to reflux 2h, filter, and are repeated 3 times, merging filtrate, are concentrated under reduced pressure to a small amount of
After volume, 95% ethanol is added, the ethanol content of solution is reached 80%, stand 24h, 3000r/min centrifugation 10min, taken out
Precipitation, as Thick many candies B obtains 67.2g after freezing.
Embodiment 3
2 kilograms of the leaf of Camellia nitidissima is taken, meal is broken into, after 10 times of 85% ethanol room temperature diacolation 24h of weight, diacolation is released
Liquid, is repeated 3 times, and merges percolate, is concentrated under reduced pressure to give alcohol extract, can separately do him and use.
The dregs of a decoction add 10 times of water of weight, are heated to reflux 2h, filter, and are repeated 3 times, merging filtrate, are concentrated under reduced pressure to a small amount of
After volume, 95% ethanol is added, the ethanol content of solution is reached 80%, stand 24h, 3000r/min centrifugation 10min, taken out
Precipitation, as Thick many candies C obtains 52.0g after freezing.
Embodiment 4
Influence pharmacological evaluation to mouse stress ability
Method：
1. on the mouse fatigue proof influence of swimming
Take SPF grades of kunming mice 70, be randomly divided into Thick many candies A, B, C high and low dose group and control group, Thick many candies A, B,
Gavage gives Thick many candies A, B, C of various dose to C high and low doses group respectively, and control group gives normal saline, continuous 7d.
All animal d8 carry out swimming test, 32 ± 2 DEG C of water temperature, the depth of water 50cm, each 20min.D22 mouse tails root heavy burden 2g swims
Swimming, record mouse power exhausts the time that (timing since mouse is put into water, can not emersion again to its head submerged 10s
Untill the water surface).
2. the influence pair mouse oxygen deficit tolerance
Take SPF grades of kunming mice 70, be randomly divided into Thick many candies A, B, C high and low dose group and control group, Thick many candies A, B,
Gavage gives Thick many candies A, B, C of various dose to C high and low doses group respectively, and control group gives normal saline, continuous 7d.
1h carries out oxygen deficit tolerance experiment after last dose：Take 1 animal and be put into the 500ml wide-mouth bottle interior sealings equipped with 20g soda limes,
Record time-to-live of the every animal in bottle.
3. the cold influence of pair hardy
SPF grades of kunming mice 70, is randomly divided into Thick many candies A, B, C high and low dose group and control group, Thick many candies A, B, C
Gavage gives Thick many candies A, B, C of various dose to high and low dose group respectively, and control group gives normal saline, continuous 7d.End
During each group mouse is put -20 DEG C of refrigerator by 1h simultaneously after secondary administration, untill control group mice death more than half.Record is each
Group animal dead number simultaneously calculates the death rate.
As a result：
Compare with control group, it is Thick many candies A, B, C high and low dose group mouse swimming time, Hypoxia under normal pressure, cold
There were significant differences that (1) P ＜ 0.01 or P ＜ 0.05, the results are shown in Table, and illustrate that Thick many candies A, B, C can agent for the death rate under cool condition
Amount dependence ground extension mouse swimming time, improves its oxygen deficit tolerance and cold resistant ability.The wherein effect of Thick many candies B
It is more notable.
The anti-stress effect of table 1 Thick many candies A, B, C
Compared with control group, * P ＜ 0.05, * * P ＜ 0.01
Claims (5)
1. a kind of preparation method of the camellia chrysantha polysaccharide with anti-stress effect, it is characterised in that：The withe or leaf of Camellia nitidissima are taken,
Meal is broken into, after 10 times of 85% ethanol room temperature diacolation 24h of weight, percolate is released, is repeated 3 times, merge percolate, decompression
Alcohol extract is concentrated to give, him can be separately done and be used；The dregs of a decoction add 10 times of water of weight, are heated to reflux 2h, filter, and are repeated 3 times, and merge filter
Liquid, is concentrated under reduced pressure to after a small amount of volume, adds 95% ethanol, the ethanol content of solution is reached 80%, stands 24h, 3000r/
Min is centrifuged 10min, takes out precipitation, as Thick many candies.
2. camellia chrysantha polysaccharide as claimed in claim 1 is preparing the application of anti-stress medicine, health food or food.
3. camellia chrysantha polysaccharide as claimed in claim 2 is preparing the application of endurance medicine, health food or food.
4. camellia chrysantha polysaccharide as claimed in claim 2 is preparing the application of anti-hypoxia medicament, health food or food.
5. camellia chrysantha polysaccharide as claimed in claim 2 is preparing the application of cold resistant medicine, health food or food.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115715747A (en) * | 2022-11-30 | 2023-02-28 | 水羊化妆品制造有限公司 | Application of dark green tea polysaccharide in preparation of anti-pressure product |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485789A (en) * | 2009-02-06 | 2009-07-22 | 广西桂人堂金花茶产业集团股份有限公司 | Camellia chrysantha polysaccharide lozenge and method for producing the same |
| CN104383123A (en) * | 2014-11-14 | 2015-03-04 | 程金生 | Method for preparing golden camellia lipid-lowering blood sugar-reducing preparation |
| CN104856061A (en) * | 2015-06-24 | 2015-08-26 | 福建省福鼎市名山茶叶有限公司 | Method for extracting anti-oxidative substance from aged white golden camellia tea |
| CN104958440A (en) * | 2015-05-22 | 2015-10-07 | 大连理工大学 | Camellia nitidissima Chi flower extract as well as preparation method and application thereof |
| CN105175569A (en) * | 2015-10-27 | 2015-12-23 | 桂林医学院 | Method for extracting camellia chrysantha polysaccharide from camellia chrysantha leaves |
| CN105175570A (en) * | 2015-10-27 | 2015-12-23 | 桂林医学院 | Method for extracting polysaccharide from camellia chrysantha leaves |
-
2017
- 2017-03-13 CN CN201710151756.XA patent/CN106866833A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485789A (en) * | 2009-02-06 | 2009-07-22 | 广西桂人堂金花茶产业集团股份有限公司 | Camellia chrysantha polysaccharide lozenge and method for producing the same |
| CN104383123A (en) * | 2014-11-14 | 2015-03-04 | 程金生 | Method for preparing golden camellia lipid-lowering blood sugar-reducing preparation |
| CN104958440A (en) * | 2015-05-22 | 2015-10-07 | 大连理工大学 | Camellia nitidissima Chi flower extract as well as preparation method and application thereof |
| CN104856061A (en) * | 2015-06-24 | 2015-08-26 | 福建省福鼎市名山茶叶有限公司 | Method for extracting anti-oxidative substance from aged white golden camellia tea |
| CN105175569A (en) * | 2015-10-27 | 2015-12-23 | 桂林医学院 | Method for extracting camellia chrysantha polysaccharide from camellia chrysantha leaves |
| CN105175570A (en) * | 2015-10-27 | 2015-12-23 | 桂林医学院 | Method for extracting polysaccharide from camellia chrysantha leaves |
Non-Patent Citations (2)
| Title |
|---|
| 张萍: "金花茶的花提取物降血脂作用研究", 《中国优秀硕士学位论文全文数据库》 * |
| 邹登峰 等: "金花茶多糖抗小鼠免疫性肝损伤作用的研究", 《华西药学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115715747A (en) * | 2022-11-30 | 2023-02-28 | 水羊化妆品制造有限公司 | Application of dark green tea polysaccharide in preparation of anti-pressure product |
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