CN101485789A - Camellia chrysantha polysaccharide lozenge and method for producing the same - Google Patents
Camellia chrysantha polysaccharide lozenge and method for producing the same Download PDFInfo
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- CN101485789A CN101485789A CNA2009101138569A CN200910113856A CN101485789A CN 101485789 A CN101485789 A CN 101485789A CN A2009101138569 A CNA2009101138569 A CN A2009101138569A CN 200910113856 A CN200910113856 A CN 200910113856A CN 101485789 A CN101485789 A CN 101485789A
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Abstract
The invention relates to a polysaccharide buccal tablet prepared from Camellia nitidissima, which mainly comprises the following raw materials: polysaccharide substances extracted from the Camellia nitidissima, a sweetening agent, trehalose, citric acid and the like. The preparation method comprises the following steps: adopting supersonic waves to leach the Camellia nitidissima first, adopting a nano-filtration method to perform concentration production of a concentrated Camellia nitidissima solution, performing spray drying, tabletting and package, and obtaining the product. The product has the functions of reducing blood sugar, blood pressure, blood fat and cholesterol in serum, inhibiting tumor growth, preventing atherosclerosis, activating various enzymes in human body, improving the immunological competence of the body, prolonging senescence, improving digestion of intestinal canals, and the like, has complete health-care functions, is suitable to be eaten by common people, and has obvious health-care effect.
Description
Technical field
The invention belongs to the health promoting product of making from the plant extract material, particularly oral buccal tablet and the production method of making from the material of plant leaf of Camellia nitidissima Chi extraction thereof.
Background technology
Camellia nitidissima Chi [Camellia chrysantha (Hu) Tuyama] is a Flos Camelliae Japonicae section Camellia Camellia nitidissima Chi group plant, and early 1960s is found first in China Guangxi, is the rare plant resources in a kind of world.Camellia nitidissima Chi gold lobe Barringtonia racemosa (L.) B L.ex Do., cured matter is golden yellow, is charming appearance and personality, and is pretty and dazzling pleasant, noble graceful.As Zhuang a kind of traditional Chinese herbal medicine among the people, Camellia nitidissima Chi is mainly used in pharyngolaryngitis, dysentery, hypertension, prophylaxis of tumours, and flower is used to have blood in stool, menoxenia.Recent study finds that leaf of Camellia nitidissima Chi contains multiple physiologically active ingredients such as tea polysaccharide, tea polyphenols, tea Saponin, flavonoid, contains abundant germanium, selenium and zinc etc. simultaneously and keeps the health beneficial trace elements.Animal and clinical experiment show that leaf of Camellia nitidissima Chi has inhibitory action to the transplantability malignant tumor, also have blood pressure lowering, blood fat reducing, cholesterol reducing and prevent effects such as atherosclerosis, along with people to the deepening continuously of Camellia nitidissima Chi research, the high activity composition of Camellia nitidissima Chi and the potentiality of the medicinal aspect of medical science are familiar with by common people gradually.
Round Camellia nitidissima Chi in pharmaceutically application, people have carried out many effective researchs and exploration to Camellia nitidissima Chi and extract thereof at present, the Wei Lu of Guangdong Ocean University and Guangxi Fuxin Science ﹠ Technology Co., Ltd. for example, Qin Xiaoming, Huang Riqiu, Huang Yingyi, Liu Zhixin is at " food science and technology " .2007,32 (11) .-100-102 have delivered " technical study of ultrasonic extraction camellia chrysantha polysaccharide " this article and by single factor and orthogonal experiment the technology of ultrasonic extraction camellia chrysantha polysaccharide have been studied, inquired into solid-liquid ratio, extract temperature, these 4 influence factors of extraction time and ultrasonic frequency, the optimum extraction process condition of having determined camellia chrysantha polysaccharide is solid-liquid ratio 1: 50,80 ℃ of temperature, time 1.0h, ultrasonic frequency is 53.2kHz, and the yield of polysaccharide is 3.1% under this condition.Simultaneously supercritical ultrasonics technology and traditional extraction are compared, the result shows the yield 1.0% that adopts ultrasonic extraction to improve tea polysaccharide.Equally, Wei Lu, Qin Xiaoming, woods Hua Juan, Ning Enchuan, Yang Hongzai " food science and technology " .2008,33 (7) .-247-249 have delivered the article of " the hypolipemic function research of camellia chrysantha polysaccharide ", utilization ultrasound wave extracting technology extracts camellia chrysantha polysaccharide, and by removing albumen, dialysing crude polysaccharides is carried out initial gross separation, with the mice of the polysaccharide feeding hyperlipidemia after the initial gross separation, inquire into the function of its blood fat reducing.The result shows: high, medium and low 3 dosage groups of camellia chrysantha polysaccharide all can reduce TC, TG and the LDL-C level in the hyperlipidemia mice serum extremely significantly, improve HDL-C/TC ratio.Irritate stomach after 2 weeks, the TC level of Camellia nitidissima Chi group mice serum all can reach the normal group level, and effect is better than XUEZHIKANG JIAONANG.The result shows that camellia chrysantha polysaccharide has tangible hypolipemic function.
Aspect Camellia nitidissima Chi making health food, we also retrieve following open source literatures, for example:
1, Chinese patent application number: 03149225.8 applying date: 2003.06.18 title: a kind of Camellia nitidissima Chi concentrated solution open (bulletin) number: CN1460438 apply for (patent right) people: Guangxi Fuxin Science ﹠ Technology Co., Ltd. address: the 8th building, Beihai Road ,Beihai City ,Guangxi Zhuang Autonomousnationality District panorama mansion east, make a summary: this disclosure of the Invention a kind of Camellia nitidissima Chi concentrated solution.At present concentrating of Camellia nitidissima Chi extracting solution all adopted conventional high temperature extraction method for concentration, under the condition of high temperature, the aromatic substance volatilization in the Camellia nitidissima Chi has been scattered and disappeared, even active component is subjected to than havoc.What the present invention adopted is that nanometer film separation concentration technique can concentrate the Camellia nitidissima Chi extracting solution under 25-40 ℃ of condition, has avoided the heating concentration process to the destruction of active ingredient and the loss of active ingredient.The present invention is the basis of carrying out the Camellia nitidissima Chi series product development, can further develop Camellia nitidissima Chi food, beverage, health product, article of everyday use and medicine.
2, Chinese patent application number: 93103963.0 applyings date: 1993.04.07 title: oral honeysuckle flower tea and production method thereof, open (bulletin) number: CN1093266 applies for (patent right) people: the Huaqiao-Hongfa Co., Ltd., Beihai City, Guangxi, summary: oral honeysuckle flower tea and production method thereof, prescription: sugar 1, Camellia nitidissima Chi concentrated solution 0.05～0.50, Mel 0.05～0.03, Radix Glycyrrhizae 0.01～0.20, Flos Chrysanthemi 0.01～0.09, water 5～20.Leaf of Camellia nitidissima Chi and water heating extraction, Cryoprecipitation 8～20 hours, wine are sunk cold putting 8～20 hours, get concentrated solution behind the evaporation ethanol, Radix Glycyrrhizae and water heating extraction, extracting solution soaks Flos Chrysanthemi and gets soak, heat in proportion with sugar, hydromel, concentrated solution and to mix, preparation filtration, packing sterilization.The present invention contains multiple human body requisite trace element, several amino acids and vitamin.
3, Chinese patent application number: 03140885.0 title: a kind of Camellia nitidissima Chi extracting solution open (bulletin) number: CN1476767, application (patent right) people: Guangxi Fuxin Science ﹠ Technology Co., Ltd., address: seat the 8th buildings, Beihai Road ,Beihai City ,Guangxi Zhuang Autonomousnationality District panorama mansion east, summary: a kind of Camellia nitidissima Chi extracting solution.Contain many compositions in the Camellia nitidissima Chi to the human body beneficial; but be not fully used for a long time; the method of existing extraction Camellia nitidissima Chi composition is high temperature extraction and concentrates; can partly destroy its effective ingredient like this; adopting alcohol extraction and water to carry combines; and employing ultrasonic wave concussion; to improve extraction efficiency; overall process has adopted and has been lower than 60 ℃ cryogenic conditions; aromatic substance in the Camellia nitidissima Chi and various compositions have effectively been protected with health care; more than the extraction rate reached to 90%, and can shorten extraction time, reduce cost.
4, [autograph] Camellia nitidissima Chi health beverage Research on processing technology [author] Ning Enchuan, Qin Xiaoming, Ning Jian, Liu Zhixin [mechanism] Guangxi University's light industry and food engineering institute, food science and technology institute of Guangdong Ocean University, Guangxi Fuxin Science ﹠ Technology Co., Ltd., [periodical name] Guangxi light industry .2007,23 (1) .-7-8, the 20[digest] inquired into the processing technique and the optimum formula of Camellia nitidissima Chi health beverage, color and luster keeps and clarifying optimization process method, develops a kind of Camellia nitidissima Chi peculiar flavour that has, and is sweet refrigerant, long times of aftertaste, and have blood lipid regulation, the Camellia nitidissima Chi health beverage of effects such as throat-clearing lung-moistening.Its technological process is: with Fructus Momordicae, Flos Sophorae, Herba Menthae-pretreatment-lixiviate+filtration-cooling-Camellia nitidissima Chi concentrated solution-dilution-debitterize (B-cyclodextrin)-allotment-filtration-homogenizing-clarification-fill-sterilization-finished product-detection.
5, research [author] Zhao Yanhua of [autograph] Processing Technology of Yellow Flower Camellia Beverage, Tian Chengrui, Xu Changbo, Wei energy, Department of Food Engineering of Qin Lihu [mechanism] Shaanxi Normal University, ] the modern beverage company limited of Xi'an Asterias amurensis Lutken, [3] Fangchenggang City Camellia nitidissima Chi company limited, Xi-an Milk Products Science Inst., [periodical name] Food Science .2005,26 (9) .-323-326[digests] this paper studied the basic technology of camellia chrysantha beverage lixiviate under the working condition, and prevented that the clarification process of shelf life muddiness from appearring in camellia chrysantha beverage.By multifactor orthogonal experiment, determined the best production technology of camellia chrysantha beverage.
6, [autograph] oral honeysuckle flower tea Research on processing technology [author] Ning Enchuan [1] Qin Xiaoming [2] Yang Hong [3] [mechanism] [1] Guangxi University's light industry and food engineering institute, [2] Marine University Of Zhanjiang's food science and technology institute, [3] Guangxi Fuxin Science ﹠ Technology Co., Ltd., [periodical name] food industry science and technology .2006,27 (1) .-121-122, the 125[digest] inquired into the processing technique and the optimum formula of oral honeysuckle flower tea, with the Camellia nitidissima Chi is raw material, adopt enzymolysis processing, handle extraction leaf of Camellia nitidissima Chi pharmaceutical components in conjunction with supersonic oscillations, filtered out the optimum formula of oral liquid by orthogonal experiment, develop a kind of Camellia nitidissima Chi peculiar flavour that has, little hardship that enters the mouth, long times of aftertaste, the tangible health promoting product of hypolipemic function.
7, [mechanism] the Southern Yangtze University Foodstuffs Academies such as research [author] Wang Yuanfeng, Wang Dengliang of [autograph] leaf of Camellia nitidissima Chi Enzymatic Extraction process conditions, Agricultural University Of South China, [periodical name] Wuxi Light Industry Univ.'s journal: food and biotechnology 2001,20 (6) .-582-587, the 593[digest] with adding exogenous enzyme the extracts active ingredients in the leaf of Camellia nitidissima Chi is come out, its optimum extraction condition is: amount of water is 100 times of leaf of Camellia nitidissima Chi dry sample, the mass fraction of the papain that adds is 0.4%, pH value is 5.0-5.5, temperature is 50-55 ℃, constant temperature 120min.With this understanding, leaf of Camellia nitidissima Chi water leaching rate, soluble sugar and amino acid content are compared respectively according to increasing by 39.47%, 7.39% and 1.84%, the amino acid whose total leaching rate of crude protein is about 25.5%, and the leaching rate of mineral element Fe, Zn, Se, Mn, Co, Mo, Ge, V is all more than 95% in the leaf of Camellia nitidissima Chi.
8, food science and technology institute of [autograph] leaf of Camellia nitidissima Chi pharmaceutical components novel technology for extracting research [author] Qin Xiaoming [1] Ning Enchuan [2] Yang Hong [3] [mechanism] [1] Marine University Of Zhanjiang, [2] Guangxi University's light industry and food engineering institute, [3] Guangxi Fuxin Science ﹠ Technology Co., Ltd., [periodical name] food industry science and technology .2005,26 (10) .-122-124[digests] purpose inquire into to adopt enzymolysis processing to handle action effect to leaf of Camellia nitidissima Chi pharmaceutical components leaching rate in conjunction with supersonic oscillations. method with tea polyphenols and total Saponin as leaching index, pass through orthogonal experiment, determine from leaf of Camellia nitidissima Chi, to extract the optimum extraction process condition of pharmaceutical components. best of breed is that ultrasonic frequency is 25kHz as a result, extract 85 ℃ of temperature, extraction time 90min; The actual effect of middle trial production shows. new technology is compared with existing production technology, and the extraction rate of tea polyphenols, total flavones and total Saponin has improved 17.3%, 13.2% and 9.3% respectively, and the extracting activity time has shortened 2h.
9, [autograph] Camellia nitidissima Chi concentrating drinking liquid Research on processing technology [author] Yang Hong [1] Qin Xiaoming [2] Ning Enchuan [3] [mechanism] [1] Guangxi Fuxin Science ﹠ Technology Co., Ltd., [2] Guangxi University of Marine University Of Zhanjiang [3] [periodical name] Guangxi tropical agriculture .2005 (3) .-38-39[digest] adopt enzymolysis processing to handle extraction leaf of Camellia nitidissima Chi pharmaceutical components in conjunction with supersonic oscillations, filter out the optimum formula of concentrating drinking liquid by orthogonal test, development has the health promoting product of Camellia nitidissima Chi peculiar flavour.
Recognize from above-mentioned open source literature, composition Study and the extraction of Camellia nitidissima Chi at present, the health promoting product of producing is more, but because several respects shortcoming is arranged with the polysaccharide component of commonsense method extraction leaf of Camellia nitidissima Chi, the one, Camellia nitidissima Chi blade wax coat is extremely thick, polysaccharide component is difficult to transfer in the extracting solution, the 2nd, the easy oxidation stain of product, though above-mentioned document discloses the technology of ultrasonic extraction camellia chrysantha polysaccharide, but extract later the concentrating of camellia chrysantha polysaccharide in the past and generally adopt vacuum concentration, heat time heating time is long, and the response rate and the activity of camellia chrysantha polysaccharide effective ingredient are lower, and the effect of making product also decreases.
Aspect resisting fatigue class health product, finding is many in the market is that the raw material extraction is made with single kind or plurality of Chinese plant, and product form mainly contains health promoting wine, oral liquid, capsule and electuary.Aspect buccal tablet, many products are also arranged, for example the U.S. buccal tablet of the health of the power source buccal tablet of new refreshing source, the continent Radix Panacis Quinquefolii buccal tablet of the herringbone board Radix Panacis Quinquefolii buccal tablet of the good happy precious health food in Guangzhou, Hubei Kang Hui Industrial Co., Ltd., Tianjin Jinshi Pharmaceutical Co., Ltd., Zhuhai Dong Jia pharmaceutcal corporation, Ltd, Heyuan City are preced with the hat health board Radix Panacis Quinquefolii buccal tablet of health ginseng and pilose antle health product company limited etc., and a lot of rules of origin are in ginseng class Chinese medicine.We can say that material source is more single.It should be noted that in addition, present most anti-fatigue health-product containing only rests on the animal efficacy test, for efficacy factor not further investigation still, product specification is lower, therefore be difficult to reach the requirement of third generation health food and integrate with world's health-product market, serious restriction the deep development of Chinese health product.
Summary of the invention
The purpose of this invention is to provide a kind of polysaccharose substance that extracts from the plant leaf of Camellia nitidissima Chi, and be made into oral buccal tablet, and the production method of this oral buccal tablet.
Camellia chrysantha polysaccharide lozenge of the present invention comprises the raw material of following composition and mass fraction:
Camellia chrysantha polysaccharide material 45-75 part;
15 parts of forming agents;
Sweeting agent 12-15 part;
Citric acid 0.5-0.8 part;
Trehalose 5-10 part;
Essence is an amount of.
Above-described camellia chrysantha polysaccharide material is with the fresh vanes lixiviate of Camellia nitidissima Chi, separate to concentrate, the material of separation and purification and spray drying gained.
Above-described forming agent comprises the wherein mixing of one or two or more kinds of carboxymethyl cellulose, modified starch, Radix Acaciae senegalis, all is to get mixed in equal amounts.Wherein modified starch is dextrin or cyclodextrin.
Above-described sweeting agent comprises sucrose, hydroxyl isomaltulose, xylitol, stevioside, Momordia grosvenori aglycone, aspartame, oligofructose or other edible sweeting agents, and these sweeting agents can adopt wherein one or more mixture.(sugariness is calculated with sucrose)
Above-described trehalose (trechalose) be by two molecule glucoses through a, the irreducibility disaccharidase of a11 keyed jointing.Learn that from Indexing of Scien. and Tech. Literature trehalose is not with can free aldehyde radical, its chemical property is stable, has the unique biological characteristic that is different from other disaccharidase.When trehalose is applied to food industry, trehalose has irreducibility, moisture retention, freezes and characteristics such as dry resistance, low sugariness, high-quality sweet taste, anti-caries and low heat value, make it can prevent that food decay is rotten, also can be fresh-keeping, even also can not cause degeneration, and can be applicable to the freeze-dried of protein, peptide, thalline etc. such as enzyme, blood complement, antibody or antigen at protein such as Temperature Treatment fish above freezing, meat.Its mechanism of action is that biological intravital trehalose has non-specific protective effect to organism and biomacromolecule.This non-specific protective effect is mainly reflected in: when biological cell is in that hunger, drying, high temperature, freezing, radiation, hyperosmosis and toxic reagent etc. are various coerces environment; Sargassum content rises rapidly in the cell; multiple biomacromolecule is all had protective effect, thus protection life itself.The main cause that trehalose is found oneself greatly elevated in fame and status is that ectogenic trehalose has good nonspecific protective effect to organism and biomacromolecule equally.Is but the trehalose of this mystery how to make food reach secular preservation actually? result from preliminary study, be because trehalose is when being used to preserve food, under the effect of room temperature, form a kind of special film and with its sealing, also can separate out mucus simultaneously with its bonding with moisten, and the original antibacterial and the antibacterial of being infected with are subsequently killed.This protecting film is highly stable under conventional humidity, and external heat radiation can also be gone out.Therefore, not only effect is very desirable to utilize trehalose to preserve food, and without what equipment, operates very simple.As long as note handling food well, in preservation, do not infiltrated by water, be not caught in the rain in transit, just the energy long preservation is not easy to go bad.The acquisition past of trehalose is to be raw material with the Sargassum, adopt organic solvent extraction, perhaps adopt methods such as ultrasonic extraction, the critical extraction of carbon dioxide to obtain, in recent years, because trehalose is used more and more widely, can undergo microbial fermentation from starch and obtain.The trehalose that the present invention selects for use does not need through handling, and direct trehalose with food stage or pharmaceutical grade is applied to prepare camellia chrysantha polysaccharide lozenge and gets final product.The inventor recognizes in test; trehalose can be protected the activity of camellia chrysantha polysaccharide; under same condition; the camellia chrysantha polysaccharide lozenge product that adds Sargassum polysaccharides; its quality, local flavor all can keep 1 year constant; the camellia chrysantha polysaccharide lozenge product that does not add Sargassum polysaccharides, active reduction by 10%/month, and it darkens.
The Chinese chemical name of above-described citric acid: 2-hydroxy propane-1,2, the 3-tricarboxylic acids here except the usefulness as acidulant, also has anticorrosion and synergic effect.
Above-described essence is the natural essence from plant extract, for example the essence of tastes such as Herba Menthae, Fructus Fragariae Ananssae, Fructus Mali pumilae, Fructus Citri tangerinae.
The production method of above-described camellia chrysantha polysaccharide oral buccal tablet is as follows:
1, raw material is handled.
The fresh blade of Camellia nitidissima Chi that harvesting is no more than 24 hours is removed yellow leaf and impurity, clean, be broken into 1cm
2Following fragment.
2, lixiviate.
Adopt the ultrasound wave extraction,, extraction temperature 60-80 ℃, extraction time 60-70 minute, under the 25-80kHz frequency, extract polysaccharide respectively with solid-liquid ratio 1:40.
3, nanofiltration membrane separation concentrates.
The solution filtration back that the ultrasound wave lixiviate is obtained concentrates under 40-60 ℃ condition, adopts the concentrated Camellia nitidissima Chi concentrated solution of producing of pressurization nano filtering process, and nanofiltration membrane component is the product of U.S. GE company, OSMONIC or KOCH company, and model is a DK series, effective area 2.5m
2, the material polyamide; A whole set of experimental provision is cleaned three parts and is formed by pretreatment, nanofiltration, and material is pressurizeed through force (forcing) pump by Raw material pail, operating pressure 1.5Mpa sends into nanofiltration membrane component, regulates the NF membrane outlet valve, make filter pressure reach predetermined value, material is divided into filtered solution and concentrated solution after NF membrane is filtered.Filtered solution enters the clear liquid jar, and concentrated solution is got back to concentration tank after assembly flows out, and so constantly circulation realizes concentrating of feed liquid and separates, filtered solution flow 1.75LPM, concentrated solution discharge 43LPM, the ultimate density index with solid quality concentration 8-10% for well.
4, camellia chrysantha polysaccharide separation and purification.
The spissated camellia chrysantha polysaccharide solution of nanofiltration membrane separation is added ethanol or glucityl azo-compound, camellia chrysantha polysaccharide is precipitated out centrifugalize (8000rpm, 15 minutes).With the dissolving of precipitate center, adjust pH value 5.0-5.5 with distilled water, in the tap water that flows, dialysed the glucityl azo-compound of removing ethanol or dissociating out 2-24 hours with citric acid.Described glucityl azo-compound primary structure is to have three phenyl ring, combines and can and be precipitated out the camellia chrysantha polysaccharide coagulation with polysaccharide-OH group by phenolic hydroxyl group.
5, spray drying; Adopt spray-dired technology to carry out drying above-mentioned concentrated solution, import pathogenic wind-warm degree is 170-180 ℃; Outlet pathogenic wind-warm degree is 90-95 ℃, and the camellia chrysantha polysaccharide powder of spray drying gained is light yellow even powdered granule, and moisture content is 3.1%.
6, film-making adds forming agent, essence, sweeting agent etc. according to above-mentioned prescription in the camellia chrysantha polysaccharide that obtains, be put into film-making in the pelleter after stirring.90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtain finished product.
Advantage of the present invention:
1, Camellia nitidissima Chi contains important trace element such as multiple polysaccharide component, aminoacid, Saponin, tea polyphenols and germanium, selenium, zinc.A large amount of medical clinical test results show, the Camellia nitidissima Chi extract has blood sugar lowering, blood pressure lowering, blood fat reducing, falls cholesterol in serum, suppresses tumor growth, prevents atherosclerosis, the human activin plurality of enzymes, improves human body immunocompetence slow down aging and improve effect such as intestinal digestive function.According to the component analysis result of Camellia nitidissima Chi, many experts infer that chemical constituents such as these effects of Camellia nitidissima Chi and tea polysaccharide have closely related.
2, adopt low-temperature ultrasonic extraction leaf of Camellia nitidissima Chi, compared with former leaf of Camellia nitidissima Chi extracting technology, because disclosed leaf of Camellia nitidissima Chi extracting technology is to continue to use traditional workshop-based Chinese herbal medicine decoction method promptly to adopt the jacketed pan high temperature extraction, the constant-pressure and high-temperature concentration technology.Adopt the product of this explained hereafter, the partly or completely forfeiture of the biological active substances of health care, the most of volatilization loss of distinctive aromatic substance; The extraction ratio of medicinal health composition is low, cause unit product raw material (bright leaf) consumption big, institute's product processed (weak flavor of low grade, have the zest abnormal flavour), quality is very unstable, and the present invention adopts the low-temperature ultrasonic abstraction technique that the polysaccharide component in the Camellia nitidissima Chi is carried out extraction separation, can reduce energy expenditure, shorten extraction time, reduce possible destruction in the leaching process, effectively improve the response rate and active maintenance of camellia chrysantha polysaccharide composition, improve extraction efficiency, higher more than 30% than traditional extraction method, purity of polysaccharide is more than 80%.
3, utilize the reaction of camellia chrysantha polysaccharide and ethanol or glucityl azo-compound, with the camellia chrysantha polysaccharide coagulation and be precipitated out, it is good to have specificity, the characteristics that separation efficiency is high, effectively improved the response rate of camellia chrysantha polysaccharide composition, activity can keep, and higher more than 30% than ethanol precipitation, purity of polysaccharide is more than 80%.
3, buccal tablet holding time of obtaining of production of the present invention is long, the active constituent content height, products such as our oral honeysuckle flower tea of once developing of past, Camellia nitidissima Chi instant tea, camellia chrysantha beverage are not because efficacy factor is separated bright, these products also only rest on the primary product stage, product specification also is difficult to promote, and does not catch up with the requirement of " third generation health product " health product.So the present invention is the clear and definite novel product of breach exploitation efficacy factor with the Camellia nitidissima Chi active polysaccharide, improve the technology content and the class of product, promote the potentiality advantage of enterprise development, and in intense market competition, try to achieve and develop.
The specific embodiment
Embodiment 1
The fresh blade of Camellia nitidissima Chi that harvesting is no more than 24 hours is removed yellow leaf and impurity, clean, be broken into 1cm
2Following fragment adopts the ultrasound wave extraction then, and with solid-liquid ratio 1:40, extraction temperature 60-80 ℃, extraction time 1-2h extracts polysaccharide respectively under the 30-80kHz frequency.The solution that then the ultrasound wave lixiviate is obtained filters the back and concentrates under 40-60 ℃ condition, adopts nano filtering process to concentrate and produces the Camellia nitidissima Chi concentrated solution; Operating pressure 1.5Mpa, filtered solution flow 1.75LPM, concentrated solution discharge 43LPM, the ultimate density index reaches 8-10% with solid quality content and gets final product, the spissated camellia chrysantha polysaccharide solution of nanofiltration membrane separation is added the glucityl azo compound, camellia chrysantha polysaccharide is precipitated out centrifugalize (8000rpm, 15 minutes).With the dissolving of precipitate center, adjust pH value 5.0-5.5 with citric acid with distilled water, dialysis is 24 hours in the tap water that flows, and removes the glucityl azo-compound that dissociates out.Carry out spray drying again, spray drying import pathogenic wind-warm degree is 170-180 ℃; Outlet pathogenic wind-warm degree is 90-95 ℃.The camellia chrysantha polysaccharide powder of spray drying gained is light yellow even powdered granule, and moisture content is 3.1%.In 50 kilograms of camellia chrysantha polysaccharides that obtain, add each 7 kilograms in carboxymethyl cellulose and dextrin, 0.5 kilogram in Herba Menthae essence, 13 kilograms of sucrose, 0.6 kilogram of citric acid, 6 kilograms of trehaloses according to above-mentioned prescription, be put into film-making in the pelleter after stirring.90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtain finished product.
Embodiment 2
The fresh blade of Camellia nitidissima Chi that harvesting is no more than 24 hours is removed yellow leaf and impurity, clean, be broken into 1cm
2Following fragment adopts the ultrasound wave extraction then, and with solid-liquid ratio 1:40, extraction temperature 60-80 ℃, extraction time 1-2h extracts polysaccharide respectively under the 30-80kHz frequency.The solution that then the ultrasound wave lixiviate is obtained filters the back and concentrates under 40-60 ℃ condition, adopts nano filtering process to concentrate and produces the Camellia nitidissima Chi concentrated solution; Operating pressure 1.5Mpa, filtered solution flow 1.75LPM, concentrated solution discharge 43LPM, the ultimate density index reaches 8-10% with solid quality content and gets final product, the spissated camellia chrysantha polysaccharide solution of nanofiltration membrane separation is added ethanol, camellia chrysantha polysaccharide is precipitated out centrifugalize (8000rpm, 15 minutes), with distilled water the precipitate center is dissolved, adjust pH value 5.0-5.5 with citric acid, dialysis is 2 hours in the tap water that flows, and removes ethanol.Carry out spray drying again, spray drying import pathogenic wind-warm degree is 170-180 ℃; Outlet pathogenic wind-warm degree is 90-95 ℃.The camellia chrysantha polysaccharide powder of spray drying gained is light yellow even powdered granule, and moisture content is 3.1%.In 50 kilograms of camellia chrysantha polysaccharides that obtain, add each 7 kilograms in carboxymethyl cellulose and dextrin, 0.5 kilogram in Herba Menthae essence, 13 kilograms of sucrose, 0.6 kilogram of citric acid, 6 kilograms of trehaloses according to above-mentioned prescription, be put into film-making in the pelleter after stirring.90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtain finished product
Embodiment 3
With 60 kilograms in the camellia chrysantha polysaccharide powder of above-mentioned spray drying gained, add each 5 kilograms in Radix Acaciae senegalis, carboxymethyl cellulose and dextrin, 0.5 kilogram of strawberry essence, 5 kilograms of hydroxyl isomaltuloses, 0.5 kilogram of citric acid, 8 kilograms of trehaloses, be put into film-making in the pelleter after stirring.90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtain finished product.
Embodiment 4
With 70 kilograms in the camellia chrysantha polysaccharide powder of above-mentioned spray drying gained, add each 8 kilograms of Radix Acaciae senegalis and carboxymethyl celluloses, 1 kilogram of Momordia grosvenori aglycone, 0.8 kilogram of citric acid, 8 kilograms of trehaloses, be put into film-making in the pelleter after stirring.90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtain finished product
Embodiment 5
With 75 kilograms in the camellia chrysantha polysaccharide powder of above-mentioned spray drying gained, add each 8 kilograms of Radix Acaciae senegalis and cyclodextrins, 0.5 kilogram of strawberry essence, 4 kilograms of aspartames, 0.6 kilogram of citric acid, 10 kilograms of trehaloses, be put into film-making in the pelleter after stirring.90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtain finished product.
Embodiment 6
With 75 kilograms in the camellia chrysantha polysaccharide powder of above-mentioned spray drying gained, add each 8 kilograms of Radix Acaciae senegalis and carboxymethyl celluloses, 0.5 kilogram of flavoring orange essence, 5 kilograms of xylitol, 0.5 kilogram of citric acid, 8 kilograms of trehaloses, be put into film-making in the pelleter after stirring.90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtain finished product.
Embodiment 7
With 70 kilograms in the camellia chrysantha polysaccharide powder of above-mentioned spray drying gained, add each 8 kilograms of dextrin and carboxymethyl celluloses, 1 kilogram of stevioside, 5 kilograms of sucrose, 0.8 kilogram of citric acid, 5 kilograms of trehaloses are put into film-making in the pelleter after stirring.90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtain finished product.
Camellia chrysantha polysaccharide lozenge of the present invention carries out the test of serum lipids in rats:
1, stock
With reference to the prescription of hyperlipemia model of rats molding in " herbal pharmacology experimental methodology " and " herbal pharmacology research methodology ", feedstuff includes 4% cholesterol, 10% Adeps Sus domestica, 0.2% methylthiouracil, 85.8% normal feedstuff.
2, experimental technique
After weighing, rat is divided into 6 groups at random: (1) high dose group (0.8g/kg), (2) dosage group (0.4g/kg) in, (3) low dose group (0.2g/kg), (4) positive controls (lovastatin, 0.01g/kg), (5) high fat matched group and (6) blank group, preceding 15d, except that the blank group was fed normal feedstuff, all the other each groups were fed the modeling feedstuff. and beginning in the 16th day, feed are still by last method, simultaneously, the camellia chrysantha polysaccharide lozenge height, in, low dose group and positive controls are by above-mentioned dosage regimen ig administration, and the administration volume is the 20mg/kg body weight, and high fat matched group and blank group ig are with the distilled water of equivalent, every day 1 time, continuous 4 weeks. when experiment finished, water 16h was can't help in fasting, 1% pentobarbital sodium intraperitoneal injection of anesthesia, the ventral aorta blood sampling, 3000r/min, centrifugal 10min gets serum and surveys T-CHOL, triglyceride, the content of high density lipoprotein (HDL-C) and low density lipoprotein, LDL (LDL-C).
3, data statistics and processing
Blood fat represents with mean X-± S, does the sided t check with the statistical software of Excel software kit, and the significance of difference is analyzed in α=0.05.
4, experimental result
After giving high fat diet, TC in the high fat control rats serum, TG and LDL-C content raise, and HDL-C descends, compare with the blank group, check through t, all has significant statistical significance, under the same experimental conditions, camellia chrysantha polysaccharide lozenge high dose group (0.8g/kg), middle dosage group (0.4g/kg) and positive controls (lovastatin, 0.01g/kg) all can significantly reduce hyperlipidemia rat model serum TC, TG and LDL-C content, compare with high fat matched group, check through t, all have significant statistical significance, and camellia chrysantha polysaccharide lozenge low dose group (0.2g/kg) effect is not obvious, high dose group and the lovastatin HDL-C content in the hyperlipidemia rat model serum that can also raise.
Following form be camellia chrysantha polysaccharide lozenge to the influence of hyperlipidemia rat model serum TC, TG, LDL-C and HDL-C content (n=10, X-± SD):
Compare ※ P＜0.05, ※ ※ P＜0.01, ※ ※ ※ P＜0.001 with high fat matched group.
Compare △ P＜0.05, △ △ P＜0.01, △ △ △ P＜0.001 with positive controls.
Compare with the blank group, ▲ P＜0.05, ▲ ▲ P＜0.01, ▲ ▲ ▲ P＜0.001.
The result of the test that camellia chrysantha polysaccharide lozenge of the present invention carries out serum lipids in rats shows, leaf of Camellia nitidissima Chi water extract height, middle dosage group all can reduce TC, TG and LDL-C content in the hyperlipidemia rat model serum, effect is suitable with the positive control drug lovastatin, compare with high fat matched group, through the t check, difference all has the significance meaning; High dose group and the lovastatin HDL-C content in the hyperlipidemia rat model serum that can also raise, prompting leaf of Camellia nitidissima Chi water extract has effect for reducing blood fat. and the hyperlipemia that animal feed satisfied fatty acid (Adeps Sus domestica) feedstuff forms is an EHL, the medicine that EHL is had a curative effect plays a role by the biosynthesis that suppresses TC, so that supposition camellia chrysantha polysaccharide lozenge effect for reducing blood fat mechanism of the present invention may be with its inhibition endogenous TC is synthetic relevant.
The edible case history of camellia chrysantha polysaccharide lozenge of the present invention:
Example 1, old * *, the civil servant, the man, 45 years old, height 168, people from Port of Fangcheng, Guangxi likes drinking on ordinary days, motion is few, and is more fat, blood fat, blood pressure are higher, ill health is often dizzy.Though take the hypertension and hyperlipemia medicine through treatment, setting in motion, effect is bad, and the back is introduced and is taken camellia chrysantha polysaccharide lozenge of the present invention, 5 of every days, every 1 gram, after 2 months, blood fat, blood pressure reduce, and the mental status has all had certain improvement.
Example 2, deep * *, the woman, 50 years old, Nanning people from city, strive for many years in factory, in the past because busy work all the year round, have no time to attend to and take good care of one's health, so that fall sick from overwork, immunity degradation, feel that health is tired, sit down and just do not remember inefficiency, energy is not abundant, it is unable upstairs to pant, easy catching a cold, hypomnesis, distraction, face is gloomy unglazed, be full of wrinkles, skin is done rough, the low quality life is lived in often constipation, take camellia chrysantha polysaccharide lozenge of the present invention, 5 of every days, every 1 gram is after 2 months, skin begins smooth high resilience, stool is normal, sleep improves, and has got back to the epoch of more than ten years when making light of one's life by commiting suicide body in the year before last again.
Example 3, Liu * *, woman, 30 ages, Guilin people from city, the workman of pharmaceutical factory, owing to often be on night shift, daytime is not sleep well, and is dizzy all day, the belly distending pain, the soreness of waist, One's face was like wax, takes camellia chrysantha polysaccharide lozenge of the present invention, 8 of every days, every 1 gram was taken 20 days, back belly distending pain, and soreness of waist symptom takes a turn for the better, after taking one month, belly distending pain, the soreness of waist symptom spirit that all disappears takes a turn for the better, and continues to take two weeks, feel good, muscle power increases, and is of pink and healthy-looking, feels good.
Example 4, Ouyang * *, the man, 7 years old, people from Beihai, Guangxi in November, 2008, had sore throat after the cat fever 2 days, took camellia chrysantha polysaccharide lozenge of the present invention, and 4 of every days, every 1 gram is not taken any Chinese medicine and western medicine, and after 2 days, the situation of having sore throat disappears.
Example 5, Wu *, woman, 40 years old, people from Qinzhou City, Guangxi, height 162,85 kilograms of body weight, because of obesity, sensation of oppression over the chest with shortness of breath, cardiopalmus are weak, take camellia chrysantha polysaccharide lozenge of the present invention, 5 of every days, every 1 gram, after 3 months, 75 kilograms of body weight, only surplus slightly breathing hard and dizzy symptom, surplus disease disappears.
Claims (5)
1, a kind of camellia chrysantha polysaccharide lozenge is characterized in that: its primary raw material composition and mass fraction are as follows:
Camellia chrysantha polysaccharide material 45-75 part;
15 parts of forming agents;
Sweeting agent 12-15 part;
Citric acid 0.5-0.8 part;
Trehalose 5-10 part;
Essence is an amount of.
2, camellia chrysantha polysaccharide lozenge according to claim 1 is characterized in that: described camellia chrysantha polysaccharide material is with the fresh vanes lixiviate of Camellia nitidissima Chi, separate to concentrate, the material of separation and purification and spray drying gained.
3, camellia chrysantha polysaccharide lozenge according to claim 1 is characterized in that: described forming agent comprises wherein one or more the mixed in equal amounts of carboxymethyl cellulose, modified starch, Radix Acaciae senegalis; Wherein said modified starch is dextrin or cyclodextrin.
4, camellia chrysantha polysaccharide lozenge according to claim 1 is characterized in that: described sweeting agent comprises wherein one or more the mixture of sucrose, hydroxyl isomaltulose, xylitol, stevioside, Momordia grosvenori aglycone, aspartame or oligofructose.
5, a kind of production method of camellia chrysantha polysaccharide lozenge as claimed in claim 1 is characterized in that:
(1) the fresh blade of the Camellia nitidissima Chi that harvesting is no more than 24 hours is removed yellow leaf and impurity, cleans, is broken into fragment;
(2) adopt the ultrasound wave extraction,, extraction temperature 60-80 ℃, extraction time 60-70 minute, under the 25-80kHz frequency, extract polysaccharide respectively with solid-liquid ratio 1: 40;
(3) solution that the ultrasound wave lixiviate is obtained filters the back and concentrates under 40-60 ℃ condition, adopt nano filtering process to concentrate and produce the Camellia nitidissima Chi concentrated solution, operating pressure 1.5Mpa, filtered solution flow 1.75LPM concentrated solution discharge 43LPM, the ultimate density index is with solid content 8-10%;
(4) the spissated camellia chrysantha polysaccharide solution of nanofiltration membrane separation is added ethanol or glucityl azo-compound, camellia chrysantha polysaccharide is precipitated out, centrifugalize, with distilled water the precipitate center is dissolved, adjust pH value 5.0-5.5 with citric acid, in the tap water that flows, dialysed 2-24 hours, remove the ethanol or the glucityl azo-compound that dissociate out;
(5) with the camellia chrysantha polysaccharide concentrated solution spray drying that is precipitated out, import pathogenic wind-warm degree is 170-180 ℃; Outlet pathogenic wind-warm degree is 90-95 ℃, and making moisture content is 3.1%;
(6) film-making adds forming agent, essence, sweeting agent etc. in the camellia chrysantha polysaccharide that obtains, be put into film-making in the pelleter after stirring, and 90-102 ℃ of drying, packing, sterilization, sampling inspection down, obtains finished product.
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