CN106749730A - It is a kind of to cut the method for helping interaction mode to improve golden flower tea extraction polysaccharide dissolution rates using micro- - Google Patents
It is a kind of to cut the method for helping interaction mode to improve golden flower tea extraction polysaccharide dissolution rates using micro- Download PDFInfo
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- CN106749730A CN106749730A CN201611185372.1A CN201611185372A CN106749730A CN 106749730 A CN106749730 A CN 106749730A CN 201611185372 A CN201611185372 A CN 201611185372A CN 106749730 A CN106749730 A CN 106749730A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses a kind of using micro- method cut and help interaction mode to improve golden flower tea extraction polysaccharide dissolution rates, belong to active components of plants extractive technique field.The method is：First golden flower tea fresh leaves are cleaned, is finished, golden camellia tea meal is obtained after dry, pulverize；Golden camellia tea meal and physics auxiliary agent are put together again after carrying out superfine grinding, micro- cutting is obtained and helps powder；Then the powder is helped to carry out ultrasonic water extraction micro- cutting, after ultrasonic extraction, extract solution carries out vacuum freeze drying, and freezing dry powder is golden camellia tea water extract；Polyoses content in extract is detected with phenol Sulphuric acid colorimetry finally to determine target component release rate.Compared with prior art, the inventive method helps interaction mode assisted extraction golden camellia tea polysaccharide using micro- cutting, it is micro- cut help powdery state under can effectively improve polysaccharide dissolubility in aqueous, further improve polysaccharide eduction rate.
Description
Technical field
The invention belongs to active components of plants extractive technique field, it is related to the extraction skill of new resource food golden flower tea components
Art, is related specifically to the extracting method of Camellia nitidissima functional active components.
Background technology
Camellia nitidissima [Camellia nitidissima Chi] category Theaceae (Theaceae) Camellia (Camellia) gold
Flower Thea section (Camellia Section Chrysantha Chang), is uniquely have golden yellow dyeing defect in camellia family
The kind of valve, is described as " tea race queen ", " plant kingdom giant panda " etc., has been listed in country-level, the rare protection in two grades of the world
Plant, and new resource food is classified as by No. 9 bulletin of the Ministry of Public Health of China in 2010.Golden flower camellias and leaf are that Zhuang nationality in Guangxi is among the people
Traditional medication, with medical value higher,《Guangxi Chinese medicine standard》Record, the nature and flavor of Camellia nitidissima are slight bitter, puckery, are put down, work(
Can be clearing heat and detoxicating, inducing diuresis to remove edema with curing mainly, for sphagitis, dysentery, ephritis, hypertension, irregular menstruation etc..In golden camellia tea
Containing various effective medicinal ingredients, pharmaceutical research proves there is anti-inflammation, and anti-oxidant, antitumor, reducing blood lipid is hypoglycemic,
The functions such as antiallergy, antianxiety depression, wherein camellia chrysantha polysaccharide are a kind of more active skull cap components of research, and it is in medicine side
The application in face has preferable application prospect, therefore the extraction of active component is separated into a technical barrier.
In the last few years, many domestic and foreign scholars constantly to plant effective active composition extract technique groped with it is excellent
Change, some new extractions and isolation technics constantly emerge in large numbers appearance, such as microwave, ultrasonic wave, overcritical, although these new technologies
There is significant progress than conventional art, but the low problem of active ingredient poorly water-soluble, availability is not solved fundamentally also
Certainly, and some technologies due to equipment it is special, be also only limitted to laboratory research, and may not apply to Industrialized Production Practice.Can
See, the imperfection of extractive technique is always to govern plant effective active composition deeper into research and the key of development and application
Problem.Therefore, the extraction efficiency of active ingredient is improved, reduces cost develops skill maturity so as to reach industrialized production
The exploitation of new technology is extremely urgent.It is micro- to cut the new method for helping interaction mode as high efficiency extraction animals and plants active component in recent years,
Its general principle is：By micro- shear clasmatosis, cell surface produces fresh tangent plane to animal-plant material cluster of grains, auxiliary agent with have
There is group or intermolecular mutual absorption or effect between effect composition, change the micro-property of active ingredient, surface can increase,
Specific surface area increases, and adsorptivity and polarity increase, by it is micro- cut help interaction mode to be obtained after processing micro- and cut help powder in water
Dissolubility is greatly improved.Patent of invention：One kind is used for effective plant component extracting process, granted patent number：
ZL200510130852.3, its main technical scheme is that the plant material that will be extracted is crushed, and is added in plant meal
The chemical assistant of 0.5-20% solid bases, solid acid, sucrose or salt compounds powder, and fragility or sharp property inert material
Processed together, from water or ethanol dissolution process product, separate and collect soluble constituents and obtain active ingredient.Therefore, plant
Thing active component extraction efficiency is low, and it is problem demanding prompt solution that elapsed time is long etc..
The content of the invention
The technical problem to be solved in the present invention is to provide micro- the cutting of golden camellia tea Polyose extraction new technology i.e. ultrasonic wave added and helps mutually
Camellia chrysantha polysaccharide is extracted as mode, it is therefore an objective to provide a kind of new method that polysaccharide dissolution rate is improved from Camellia nitidissima.The technology is comprehensive
Close and help effective combination of interaction mode to improve golden flower tea extractions recovery rate and polyoses content with micro- cutting using ultrasonic technique, reduce
The consumption of organic solvent, improves the problem of environmental pollution for existing.
Specifically, of the present invention cut the method for helping interaction mode to improve golden flower tea extraction polysaccharide dissolutions using micro-, with
Golden camellia tea is raw material, is comprised the following steps：
(1) golden flower tea fresh leaves are taken, is cleaned, microwave high-temperature de-enzyme, vacuum condition is dried, then by dried golden camellia tea
Crush, cross 60~200 mesh sieves, obtain golden camellia tea powder；
(2) golden camellia tea powder and physics auxiliary agent 1%~4% are put into vibromill and are ground by mass percentage
10min, obtains micro- cutting and helps powder；
(3) micro- cutting is helped into powder and water by solid-liquid ratio 1g:15ml is sufficiently mixed, frequency be 40KHz, power be 100W, temperature
Ultrasonic extraction 30min under the ul-trasonic irradiation for 70 DEG C is spent, extract solution is filtered, filtrate is collected to obtain；
(4) filtrate is carried out into freeze-drying by vacuum freeze drier, reclaims extractant-water, obtain golden camellia tea water
Extract dry powder；
(5) by golden camellia tea dry composition into certain density solution to be measured, with glucose as a standard product, using phenol-
Sulphuric acid colorimetry is detected to polyoses content in extract.
The present invention extracts new technology as camellia chrysantha polysaccharide, can effectively extract golden flower tea extractions and polyoses content, 20g
Raw material can obtain extract 3.65g~3.95g, and 1.93 times are improved compared with traditional extraction process, and polyoses content reaches
130.52mg/g~150.67mg/g, improves 2.55 times compared with traditional extraction process.Using ultrasound assisted extraction, by original
Immersion 24h shorten to 30min, greatly reduces polysaccharide dissolution time, saves the operating time, improves extraction efficiency.Micro- cutting helps interaction
The use of mode so that the dissolution rate of polysaccharide is improved and effectively protects the integrality of polysaccharide, substantially increases the recovery rate of polysaccharide.
Present invention process makees simple, cost-effective, is more beneficial for industrialization.
Brief description of the drawings
Fig. 1 is the golden camellia tea particle size distribution figure of different pulverization process.
Fig. 2A is 60 mesh sieve golden camellia tea particulate scan electron microscopes.
Fig. 2 B are 200 mesh sieve golden camellia tea particulate scan electron microscopes.
Fig. 2 C are that micro- cutting processes golden camellia tea particulate scan electron microscope.
Fig. 2 D are that micro- cutting helps treatment golden camellia tea particulate scan electron microscope.
Fig. 3 is different pulverization process golden camellia tea extract recovery rate figures.
Fig. 4 A are glucose standard curve figures.
Fig. 4 B are polyoses content figures in different pulverization process golden camellia tea extracts.
Specific embodiment
Specific embodiment of the invention is described in detail below in conjunction with technical scheme and accompanying drawing 1-4B.
Embodiment 1
Using micro- method cut and help interaction mode to improve golden flower tea extraction polysaccharide dissolution rates, with golden camellia tea as raw material,
Comprise the following steps：
(1) golden flower tea fresh leaves are taken, is cleaned, microwave high-temperature de-enzyme, vacuum condition is dried, then by dried golden camellia tea
Crush, cross 60 mesh sieves, obtain golden camellia tea powder；
(2) the mesh powder 20g of golden camellia tea 60 is taken, by solid-liquid ratio 1g:15ml adds 300mL water to be sufficiently mixed, and is in frequency
40KHz, power are ultrasonic extraction 30min under 100W, the ul-trasonic irradiation that temperature is 70 DEG C, filter extract solution, collect to obtain filtrate；
(4) filtrate is carried out into freeze-drying by vacuum freeze drier, reclaims extractant-water, obtain golden camellia tea water
Extract dry powder；
(5) by golden camellia tea dry composition into certain density solution to be measured, with glucose as a standard product, using phenol-
Sulphuric acid colorimetry is detected to polyoses content in extract.
Embodiment 2
Using micro- method cut and help interaction mode to improve golden flower tea extraction polysaccharide dissolution rates, with golden camellia tea as raw material,
Comprise the following steps：
(1) golden flower tea fresh leaves are taken, is cleaned, microwave high-temperature de-enzyme, vacuum condition is dried, then by dried golden camellia tea
Crush, cross 200 mesh sieves, obtain golden camellia tea powder；
(2) the mesh powder 20g of golden camellia tea 200 is taken, by solid-liquid ratio 1g:15ml adds 300mL water to be sufficiently mixed, and is in frequency
40KHz, power are ultrasonic extraction 30min under 100W, the ul-trasonic irradiation that temperature is 70 DEG C, filter extract solution, collect to obtain filtrate；
(4) filtrate is carried out into freeze-drying by vacuum freeze drier, reclaims extractant-water, obtain golden camellia tea water
Extract dry powder；
(5) by golden camellia tea dry composition into certain density solution to be measured, with glucose as a standard product, using phenol-
Sulphuric acid colorimetry is detected to polyoses content in extract.
Embodiment 3
Using micro- method cut and help interaction mode to improve golden flower tea extraction polysaccharide dissolution rates, with golden camellia tea as raw material,
Comprise the following steps：
(1) golden flower tea fresh leaves are taken, is cleaned, microwave high-temperature de-enzyme, vacuum condition is dried, then by dried golden camellia tea
Crush, cross 60 mesh sieves, obtain golden camellia tea powder；
(2) the mesh powder 500g of golden camellia tea powder 60 is taken, is put into vibromill and is ground 10min, obtained and micro- cut powder；
(3) take that golden camellia tea is micro- to cut powder 20g, by solid-liquid ratio 1g:15ml adds 300mL water to be sufficiently mixed, and is in frequency
40KHz, power are ultrasonic extraction 30min under 100W, the ul-trasonic irradiation that temperature is 70 DEG C, filter extract solution, collect to obtain filtrate；
(4) filtrate is carried out into freeze-drying by vacuum freeze drier, reclaims extractant-water, obtain golden camellia tea water
Extract dry powder；
(5) by golden camellia tea dry composition into certain density solution to be measured, with glucose as a standard product, using phenol-
Sulphuric acid colorimetry is detected to polyoses content in extract.
Embodiment 4
Using micro- method cut and help interaction mode to improve golden flower tea extraction polysaccharide dissolution rates, with golden camellia tea as raw material,
Comprise the following steps：
(1) golden flower tea fresh leaves are taken, is cleaned, microwave high-temperature de-enzyme, vacuum condition is dried, then by dried golden camellia tea
Crush, cross 60 mesh sieves, obtain golden camellia tea powder；
(2) the mesh powder 500g of golden camellia tea powder 60 is taken, 2% physics auxiliary agent 5g is added by mass percentage, is put into vibromill
10min is ground, micro- cutting is obtained and is helped powder；
(3) take micro- the cutting of golden camellia tea and help powder 20g, by solid-liquid ratio 1g:15ml adds 300mL water to be sufficiently mixed, and is in frequency
40KHz, power are ultrasonic extraction 30min under 100W, the ul-trasonic irradiation that temperature is 70 DEG C, filter extract solution, collect to obtain filtrate；
(4) filtrate is carried out into freeze-drying by vacuum freeze drier, reclaims extractant-water, obtain golden camellia tea water
Extract dry powder；
(5) by golden camellia tea dry composition into certain density solution to be measured, with glucose as a standard product, using phenol-
Sulphuric acid colorimetry is detected to polyoses content in extract.
Claims (2)
- It is 1. a kind of to cut the method for helping interaction mode to improve golden flower tea extraction polysaccharide dissolution rates using micro-,With golden camellia tea as raw material, it is characterised in that comprise the following steps：(1) golden flower tea fresh leaves are taken, is cleaned, microwave high-temperature de-enzyme, vacuum condition is dried, then by dried golden camellia tea powder It is broken, 60~200 mesh sieves are crossed, obtain golden camellia tea powder；(2) golden camellia tea powder and physics auxiliary agent 1%~4% are put into vibromill and are ground 10min by mass percentage, obtained Micro- cutting helps powder；(3) micro- cutting is helped into powder and water by solid-liquid ratio 1g:15ml is sufficiently mixed, and is that 40KHz, power are that 100W, temperature are in frequency Ultrasonic extraction 30min under 70 DEG C of ul-trasonic irradiation, filters extract solution, collects to obtain filtrate；(4) filtrate is carried out into freeze-drying by vacuum freeze drier, reclaims extractant-water, obtain the extraction of golden camellia tea water Thing dry powder；(5) by golden camellia tea dry composition into certain density solution to be measured, with glucose as a standard product, using phenolsulfuric acid Colorimetric method is detected to polyoses content in extract.
- 2. micro- the cutting of a kind of use according to claims 1 helps interaction mode to improve golden flower tea extraction polysaccharide dissolution rates Method, it is characterised in that described physics auxiliary agent is sharp property material-oyster shell whiting.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485789A (en) * | 2009-02-06 | 2009-07-22 | 广西桂人堂金花茶产业集团股份有限公司 | Camellia chrysantha polysaccharide lozenge and method for producing the same |
| CN104383123A (en) * | 2014-11-14 | 2015-03-04 | 程金生 | Method for preparing golden camellia lipid-lowering blood sugar-reducing preparation |
| CN104958440A (en) * | 2015-05-22 | 2015-10-07 | 大连理工大学 | Camellia nitidissima Chi flower extract as well as preparation method and application thereof |
-
2016
- 2016-12-20 CN CN201611185372.1A patent/CN106749730A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485789A (en) * | 2009-02-06 | 2009-07-22 | 广西桂人堂金花茶产业集团股份有限公司 | Camellia chrysantha polysaccharide lozenge and method for producing the same |
| CN104383123A (en) * | 2014-11-14 | 2015-03-04 | 程金生 | Method for preparing golden camellia lipid-lowering blood sugar-reducing preparation |
| CN104958440A (en) * | 2015-05-22 | 2015-10-07 | 大连理工大学 | Camellia nitidissima Chi flower extract as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 许子竞等: "金花茶叶多糖超声波辅助提取工艺优化和含量测定", 《食品科学》 * |
| 韦璐等: "超声波提取金花茶多糖的工艺研究", 《食品科技》 * |
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