CN105766614A - Method for rapidly cultivating aroma type new-variety rice - Google Patents
Method for rapidly cultivating aroma type new-variety rice Download PDFInfo
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- CN105766614A CN105766614A CN201610117880.XA CN201610117880A CN105766614A CN 105766614 A CN105766614 A CN 105766614A CN 201610117880 A CN201610117880 A CN 201610117880A CN 105766614 A CN105766614 A CN 105766614A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for rapidly cultivating aroma type new-variety rice and belongs to the technical field of new rice variety breeding.The method includes the steps of 1, parent generation; 2, backcross; 3, aroma authentication; 4, cultivation and seed harvesting; 5, shaping; 6, seed aroma detection.The method for cultivating aroma type new-variety rice has the advantages of being simple in operation, small in workload, rapid and efficient.
Description
Technical field
The invention belongs to new rice variety selection field, a kind of method being specifically related to quickly breeding odor type new varieties Oryza sativa L..
Background technology
Fragrant rice is a kind of Special Rices with natural flavor, and owing to it has, mouthfeel is good, quality better, nourishing healthy.Economic worth advantages of higher, thus it is favored by consumers, at present, on domestic and international market, the more common rice of price of scented rice is high, there is good economic benefit, for this, the cultivation of fragrant rice is always up the focus in rice cultivating field, but the selection of existing fragrant rice new varieties is generally adopted conventional hybridization method, the cultivation time is long, inefficient.
Summary of the invention
It is an object of the invention to provide a kind of cultivation time short, the breeding method of the odor type new varieties Oryza sativa L. that success rate is high.
The method of technical solution of the present invention a kind of quickly breeding odor type new varieties Oryza sativa L., comprises the following steps:
(1) parents: select fragrant rice fragrant rice non-with high yield to hybridize, obtain cenospecies F0；
(2) backcross: the cenospecies F that sowing obtains through step (1)0, carry out backcrossing obtaining the seed F that backcrosses with former aromatic rice in florescence1；
(3) fragrance is identified: to F1Seedling, when the length of time rice seedlings grow reaches 30 days, every strain rice shoot takes the blade of identical weight and adopts KOH infusion method to carry out fragrance qualification；
(4) cultivation and sowing: dulcet rice shoot individual plant is transplanted, normal management, according to plant height, every total grain panicle number, setting percentage and mass of 1000 kernel after maturation, the plant results seed that selection traits is good, and obtained seed is numbered by individual plant；
(5) sizing: the every strain of step (4) obtained seed is randomly selected 50 and carries out seeding and growing seedling, repeat (3) (4) and carry out blade fragrance qualification and seed harvest, after the Agronomic trait compiling number plant is stable, the every strain of stabilizing material randomly chooses selection 50 strains and carries out blade fragrance detection, determine that all plant are odor type plant, individual plant results seed after maturation；
(6) seed fragrance detection: in the seed that step (5) is gathered in the crops, every individual plant randomly selects 100 seeds, carries out seed fragrance qualification, each single-strain seed is all odor type and selection-breeding success.
Preferably, the method of a kind of quickly breeding odor type new varieties Oryza sativa L. of the present invention, leaf weight described in described step (3) is 2g, described KOH infusion method is: shredded by blade, putting in beaker, implantation quality mark is the KOH solution 10ml of 1.7%, builds beaker with rupture disk, after left at room temperature 10min, open rupture disk and hear its abnormal smells from the patient one by one.
Preferably, the method for a kind of quickly breeding odor type new varieties Oryza sativa L. of the present invention, described step (5) Leaf fragrance detects and the same step of step (6) seed fragrance detection method (3).
The technology of the present invention beneficial effect:
Technical solution of the present invention carries out hybridization by odor type parent and non-odor type parent and obtains cenospecies, and backcrossed with odor type parent by cenospecies, and screened by the detection of blade fragrance, technical solution of the present invention is simple to operate, workload is less, can rapidly and efficiently, directed be predictably bred as fragrant rice new varieties.
Detailed description of the invention
For ease of skilled artisan understands that technical solution of the present invention, in conjunction with embodiment, technical solution of the present invention is described further.
(1) select continuous fragrant 1A to hybridize with association excellent 335, obtain cenospecies F026；
(2) the cenospecies F obtained through step (1) is sowed0, carry out backcrossing obtaining the seed F that backcrosses with silk floss perfume (or spice) 1A in florescence12000；
(3) to F1Seedling, when the length of time rice seedlings grow reaches 30 days, every strain rice shoot takes 2g blade and adopts KOH infusion method to carry out fragrance qualification, shredding by blade, put in beaker, implantation quality mark is the KOH solution 10ml of 1.7%, beaker is built with rupture disk, after left at room temperature 10min, open rupture disk and hear its abnormal smells from the patient one by one, obtain the strain of dulcet plant 1351；
(4) dulcet rice shoot individual plant is transplanted, normal management, according to plant height, every total grain panicle number, setting percentage and mass of 1000 kernel after maturation, the plant results seed that selection traits is good, and obtained seed is numbered by individual plant, totally 34 individual plants；
(5) the every strain of step (4) obtained seed is randomly selected 50 and carries out seeding and growing seedling, repeat (3) (4) and carry out blade fragrance qualification and seed harvest, wait to compile number plant (F5) Agronomic trait stable after, gather in the crops seed；
(6) plantation step (5) obtained seed, randomly chooses selection 50 strains and carries out blade fragrance detection, and the same step of detection method (3) determines that all plant are odor type plant, individual plant results seed after maturation；
(7) in the seed that step (6) is gathered in the crops, every individual plant randomly selects 100 seeds, carry out seed fragrance qualification, put in beaker by seed, implantation quality mark is the KOH solution 10ml of 1.7%, builds beaker with rupture disk, after left at room temperature 10min, open rupture disk and hear its abnormal smells from the patient one by one, through checking each single-strain seed to be all odor type and selection-breeding success.
Invention is exemplarily described by technical solution of the present invention above in conjunction with embodiment; the obvious present invention implements and is not subject to the restrictions described above; as long as have employed the various unsubstantialities improvement that the design of the method for the present invention carries out with technical scheme; or the not improved design by invention and technical scheme directly apply to other occasion, all within protection scope of the present invention.
Claims (3)
1. the method for a quickly breeding odor type new varieties Oryza sativa L., it is characterised in that comprise the following steps:
(1) parents: select fragrant rice fragrant rice non-with high yield to hybridize, obtain cenospecies F0；
(2) backcross: the cenospecies F that sowing obtains through step (1)0, carry out backcrossing obtaining the seed F that backcrosses with former aromatic rice in florescence1；
(3) fragrance is identified: to F1Seedling, when the length of time rice seedlings grow reaches 30 days, every strain rice shoot takes the blade of identical weight and adopts KOH infusion method to carry out fragrance qualification；
(4) cultivation and sowing: dulcet rice shoot individual plant is transplanted, normal management, according to plant height, every total grain panicle number, setting percentage and mass of 1000 kernel after maturation, the plant results seed that selection traits is good, and obtained seed is numbered by individual plant；
(5) sizing: the every strain of step (4) obtained seed is randomly selected 50 and carries out seeding and growing seedling, repeat (3) (4) and carry out blade fragrance qualification and seed harvest, after the Agronomic trait compiling number plant is stable, the every strain of stabilizing material randomly chooses selection 50 strains and carries out blade fragrance detection, determine that all plant are odor type plant, individual plant results seed after maturation；
(6) seed fragrance detection: in the seed that step (5) is gathered in the crops, every individual plant randomly selects 100 seeds, carries out seed fragrance qualification, each single-strain seed is all odor type and selection-breeding success.
2. the method for a kind of quickly breeding odor type new varieties Oryza sativa L. according to claim 1, it is characterized in that, step (3) described leaf weight is 2g, described KOH infusion method is: shredded by blade, putting in beaker, implantation quality mark is the KOH solution 10ml of 1.7%, builds beaker with rupture disk, after left at room temperature 10min, open rupture disk and hear its abnormal smells from the patient one by one.
3. the method for a kind of quickly breeding odor type new varieties Oryza sativa L. according to claim 1, it is characterised in that the detection of described step (5) Leaf fragrance and the same step of step (6) seed fragrance detection method (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610117880.XA CN105766614A (en) | 2016-03-03 | 2016-03-03 | Method for rapidly cultivating aroma type new-variety rice |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610117880.XA CN105766614A (en) | 2016-03-03 | 2016-03-03 | Method for rapidly cultivating aroma type new-variety rice |
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| CN201610117880.XA Pending CN105766614A (en) | 2016-03-03 | 2016-03-03 | Method for rapidly cultivating aroma type new-variety rice |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234208A (en) * | 2016-08-05 | 2016-12-21 | 湖北省农业科学院粮食作物研究所 | A kind of fast breeding method of odor type top grade high-grade rice |
| CN109541137A (en) * | 2018-11-21 | 2019-03-29 | 广东海洋大学 | A kind of Rice Cropping cultivation detection rice scent rapid detection method |
| CN111727877A (en) * | 2020-07-29 | 2020-10-02 | 重庆市农业科学院 | Method for cultivating high-quality dual-purpose genic male sterile line of fragrant rice |
| CN114667926A (en) * | 2022-04-24 | 2022-06-28 | 安徽真金彩种业有限责任公司 | Method for cultivating new aromatic rice variety |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524007A (en) * | 2012-02-08 | 2012-07-04 | 湖南省水稻研究所 | Method for quickly breeding new aromatic rice variety |
| CN104542249A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Novel method for quickly trans-breeding northern aromatic japonica rice variety |
| CN105210859A (en) * | 2015-11-09 | 2016-01-06 | 合肥丰乐种业股份有限公司 | A kind of efficient selection of odor type anti-rice blast rice new varieties |
-
2016
- 2016-03-03 CN CN201610117880.XA patent/CN105766614A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524007A (en) * | 2012-02-08 | 2012-07-04 | 湖南省水稻研究所 | Method for quickly breeding new aromatic rice variety |
| CN104542249A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Novel method for quickly trans-breeding northern aromatic japonica rice variety |
| CN105210859A (en) * | 2015-11-09 | 2016-01-06 | 合肥丰乐种业股份有限公司 | A kind of efficient selection of odor type anti-rice blast rice new varieties |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234208A (en) * | 2016-08-05 | 2016-12-21 | 湖北省农业科学院粮食作物研究所 | A kind of fast breeding method of odor type top grade high-grade rice |
| CN106234208B (en) * | 2016-08-05 | 2018-03-16 | 湖北省农业科学院粮食作物研究所 | A kind of fast breeding method of odor type top grade high-grade rice |
| CN109541137A (en) * | 2018-11-21 | 2019-03-29 | 广东海洋大学 | A kind of Rice Cropping cultivation detection rice scent rapid detection method |
| CN111727877A (en) * | 2020-07-29 | 2020-10-02 | 重庆市农业科学院 | Method for cultivating high-quality dual-purpose genic male sterile line of fragrant rice |
| CN114667926A (en) * | 2022-04-24 | 2022-06-28 | 安徽真金彩种业有限责任公司 | Method for cultivating new aromatic rice variety |
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