CN105722967B - 用于处理细胞培养物的方法和系统 - Google Patents

用于处理细胞培养物的方法和系统 Download PDF

Info

Publication number
CN105722967B
CN105722967B CN201480062402.2A CN201480062402A CN105722967B CN 105722967 B CN105722967 B CN 105722967B CN 201480062402 A CN201480062402 A CN 201480062402A CN 105722967 B CN105722967 B CN 105722967B
Authority
CN
China
Prior art keywords
conduit
pump
cells
days
flow
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201480062402.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN105722967A (zh
Inventor
周航
B·赖特
M·于
尹进
K·康斯坦丁诺夫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51660627&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN105722967(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corp filed Critical Genzyme Corp
Priority to CN202110234907.4A priority Critical patent/CN113444620B/zh
Publication of CN105722967A publication Critical patent/CN105722967A/zh
Application granted granted Critical
Publication of CN105722967B publication Critical patent/CN105722967B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Water Supply & Treatment (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201480062402.2A 2013-09-16 2014-09-16 用于处理细胞培养物的方法和系统 Active CN105722967B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110234907.4A CN113444620B (zh) 2013-09-16 2014-09-16 用于处理细胞培养物的方法和系统

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361878502P 2013-09-16 2013-09-16
US61/878,502 2013-09-16
PCT/US2014/055897 WO2015039115A1 (en) 2013-09-16 2014-09-16 Methods and systems for processing a cell culture

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202110234907.4A Division CN113444620B (zh) 2013-09-16 2014-09-16 用于处理细胞培养物的方法和系统

Publications (2)

Publication Number Publication Date
CN105722967A CN105722967A (zh) 2016-06-29
CN105722967B true CN105722967B (zh) 2021-03-19

Family

ID=51660627

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202110234907.4A Active CN113444620B (zh) 2013-09-16 2014-09-16 用于处理细胞培养物的方法和系统
CN201480062402.2A Active CN105722967B (zh) 2013-09-16 2014-09-16 用于处理细胞培养物的方法和系统

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN202110234907.4A Active CN113444620B (zh) 2013-09-16 2014-09-16 用于处理细胞培养物的方法和系统

Country Status (17)

Country Link
US (2) US20150158907A1 (enExample)
EP (1) EP3047013B1 (enExample)
JP (4) JP6925127B2 (enExample)
KR (3) KR20230142653A (enExample)
CN (2) CN113444620B (enExample)
AU (4) AU2014318420B2 (enExample)
BR (2) BR112016005545B1 (enExample)
CA (1) CA2924332C (enExample)
CY (1) CY1124684T1 (enExample)
DK (1) DK3047013T3 (enExample)
EA (2) EA031652B1 (enExample)
ES (1) ES2891728T3 (enExample)
HU (1) HUE055888T2 (enExample)
IL (1) IL244552B (enExample)
MX (2) MX384020B (enExample)
SG (2) SG10201803601SA (enExample)
WO (1) WO2015039115A1 (enExample)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014218723B2 (en) 2013-02-22 2019-12-05 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
EP3460049A1 (en) 2013-02-22 2019-03-27 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
CN112588116A (zh) 2014-05-13 2021-04-02 美国安进公司 用于过滤器和过滤过程的过程控制系统和方法
TWI743024B (zh) 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
SG10201801943SA (en) 2014-10-24 2018-05-30 Genzyme Corp Integrated continuous isolation of fluid streams from sterile process vessels
US11306341B2 (en) 2014-12-22 2022-04-19 Genzyme Corporation Methods of culturing a mammalian cell
JP6457338B2 (ja) * 2015-05-28 2019-01-23 株式会社日立製作所 液体還流容器、細胞濃縮装置及び細胞濃縮システム
EP4310503A3 (en) 2015-12-30 2024-03-20 Momenta Pharmaceuticals, Inc. Methods related to biologics
ITUB20160272A1 (it) * 2016-01-22 2017-07-22 Univ Degli Studi Di Palermo Bioreattore a perfusione autosufficiente monouso per crescite cellulari 3D
WO2017160739A1 (en) * 2016-03-14 2017-09-21 Pendo TECH Processing system for multiple tangential flow filtration stations in bioprocessing applications
US12157877B2 (en) * 2016-04-15 2024-12-03 Boehringer Ingelheim International Gmbh Cell retention device and method
US11560539B2 (en) 2016-07-19 2023-01-24 The Automation Partnership (Cambridge) Limited Reversible liquid filtration system
EP3487981B1 (en) 2016-07-19 2022-06-08 The Automation Partnership (Cambridge) Limited Liquid filtration and pump system
CN106047707B (zh) * 2016-08-03 2018-11-13 广州赛泊泰生物技术有限公司 贴壁/悬浮型细胞培养单元、装置、系统及方法
TWI675696B (zh) 2017-06-01 2019-11-01 美商Ｅｍｄ密理博公司 用於灌注應用之切向流過濾裝置
EP3697887B1 (en) 2017-10-16 2022-04-06 Regeneron Pharmaceuticals, Inc. Perfusion methods
JP7237964B2 (ja) * 2017-11-22 2023-03-13 コーニング インコーポレイテッド 灌流バイオリアクターシステムおよび灌流細胞培養方法
US11371007B2 (en) * 2018-02-09 2022-06-28 Global Life Sciences Solutions Usa Llc System and method for fluid flow management in a bioprocessing system
WO2019155026A1 (en) * 2018-02-09 2019-08-15 General Electric Company Systems and methods for bioprocessing
US12252682B2 (en) 2018-02-09 2025-03-18 Global Life Sciences Solutions Usa Llc System and method for fluid flow management in a bioprocessing system
US11414639B2 (en) 2018-02-09 2022-08-16 Global Life Sciences Solutions Usa Llc Bioprocessing vessel
US12077743B2 (en) 2018-02-09 2024-09-03 Global Life Sciences Solutions Usa Llc Apparatus for fluid line management in a bioprocessing system
US11932842B2 (en) 2018-02-09 2024-03-19 Global Life Sciences Solutions Usa Llc Bioprocessing apparatus
US11920119B2 (en) 2018-02-09 2024-03-05 Global Life Sciences Solutions Usa Llc Systems and methods for bioprocessing
CN110241012B (zh) * 2018-03-09 2022-11-01 嘉和生物药业有限公司 一种生物大分子上游分阶段截留的生产方法、生产模块及在生产中的应用
TWI846694B (zh) * 2018-05-04 2024-07-01 美商健臻公司 具有過濾系統的灌注式生物反應器
WO2019229764A1 (en) * 2018-06-01 2019-12-05 Gennova Biopharmaceuticals Limited Process for production of recombinant tnk-tpa by packed-bed perfusion system
EP3843884A1 (en) * 2018-08-29 2021-07-07 Cytiva Sweden AB System for filtration and associated method thereof
US20210222109A1 (en) * 2018-09-06 2021-07-22 Momenta Pharmaceuticals, Inc. Methods of continuous cell culture
US12195715B2 (en) 2018-09-21 2025-01-14 Cytiva Sweden Ab Perfusion bioprocessing system and method of operating the same
JP7707062B2 (ja) * 2018-11-02 2025-07-14 ウーシー バイオロジクス アイルランド リミテッド 連続的採取を行い細胞流出を行わない強化灌流による細胞培養法
US10647954B1 (en) 2018-11-15 2020-05-12 Flaskworks, Llc Dendritic cell generating apparatus and method
IL284374B2 (en) 2018-12-31 2025-10-01 Momenta Pharmaceuticals Inc Methods for producing ozatekinumab
MX2021008758A (es) * 2019-01-25 2021-08-24 Becton Dickinson Co Metodos y aparatos para extraer selectivamente constituyentes de muestras biologicas.
CA3131430A1 (en) * 2019-04-01 2020-10-08 Boehringer Ingelheim International Gmbh Integrated and continuous recombinant protein manufacturing
WO2020258706A1 (zh) * 2019-06-28 2020-12-30 信达生物制药(苏州)有限公司 组合式层析装置、无缝连接层析方法和生物制剂的纯化方法
US11566217B2 (en) * 2019-08-13 2023-01-31 Flaskworks, Llc Duty cycle for cell culture systems
IL292337A (en) 2019-10-21 2022-06-01 Flaskworks Llc Systems and methods for cell culturing
EP3878542A1 (en) * 2020-03-11 2021-09-15 Bayer AG Filter membranes as antifoam level safeguards
KR102230957B1 (ko) * 2020-05-18 2021-03-23 경북대학교 산학협력단 Ev 여과장치
WO2022011145A1 (en) * 2020-07-08 2022-01-13 Vascular Perfusion Solutions, Inc. Reciprocating pressure perfusion system and methods
EP4182427A1 (en) * 2020-07-15 2023-05-24 Global Life Sciences Solutions USA LLC Bioprocessing perfusion system having a plurality of filters and method of operating the same
CN114058505B (zh) * 2020-07-30 2024-10-01 环球生命科技咨询美国有限责任公司 新型灌流培养用高密度微载体截留装置和使用方法
US20220119759A1 (en) * 2020-10-19 2022-04-21 Emd Millipore Corporation Cascade Tangential Flow Filtration Systems for Perfusion of Cell Culture
US11033495B1 (en) 2021-01-22 2021-06-15 Pacira Pharmaceuticals, Inc. Manufacturing of bupivacaine multivesicular liposomes
US11357727B1 (en) 2021-01-22 2022-06-14 Pacira Pharmaceuticals, Inc. Manufacturing of bupivacaine multivesicular liposomes
US11278494B1 (en) 2021-01-22 2022-03-22 Pacira Pharmaceuticals, Inc. Manufacturing of bupivacaine multivesicular liposomes
US12151024B2 (en) 2021-01-22 2024-11-26 Pacira Pharmaceuticals, Inc. Manufacturing of bupivacaine multivesicular liposomes
CN112831416B (zh) * 2021-01-29 2025-01-10 上海睿钰生物科技有限公司 一种体外生命维持灌流培养系统及其控制方法
WO2022221626A2 (en) 2021-04-16 2022-10-20 Repligen Corporation Filtration system and method
WO2022229381A1 (en) * 2021-04-29 2022-11-03 Univercells Technologies Sa Systems and methods for the continuous production and purification of biologics
WO2023287707A1 (en) 2021-07-15 2023-01-19 Just-Evotec Biologics, Inc. Bidirectional tangential flow filtration (tff) perfusion system
GB2627096A (en) 2021-10-14 2024-08-14 Pacira Pharmaceuticals Inc Bupivacaine multivesicular liposome formulations and uses thereof
EP4174164A1 (en) * 2021-10-21 2023-05-03 Alit Biotech (Shanghai) Co., Ltd. Biological filtration systems and controlling methods thereof
EP4268943A1 (en) 2022-04-27 2023-11-01 Levitronix GmbH A device for tangential flow filtration of a fluid
EP4608965A1 (en) * 2022-10-26 2025-09-03 National Resilience, Inc. Bioreactor systems and methods of operating same
WO2025122785A1 (en) * 2023-12-05 2025-06-12 Massachusetts Institute Of Technology Magnetic resonance relaxometry bioreactor monitoring
WO2025134151A1 (en) * 2023-12-20 2025-06-26 Omnibrx Biotechnologies Private Limited An apparatus for cell retention and method thereof
WO2025164695A1 (ja) * 2024-02-01 2025-08-07 国立大学法人東京科学大学 細胞培養装置及びそれを用いた細胞培養方法
US12280149B1 (en) 2024-05-20 2025-04-22 Pacira Pharmaceuticals, Inc. Manufacturing of bupivacaine multivesicular liposomes
US12251472B1 (en) 2024-05-20 2025-03-18 Pacira Pharmaceuticals, Inc. Manufacturing of bupivacaine multivesicular liposomes
US12246092B1 (en) 2024-05-20 2025-03-11 Pacira Pharmaceuticals, Inc. Manufacturing of bupivacaine multivesicular liposomes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6022742A (en) * 1986-11-26 2000-02-08 Kopf; Henry B. Culture device and method
WO2013109520A1 (en) * 2012-01-18 2013-07-25 Bayer Healthcare Llc Perfusion bioreactor systems and methods of operating the same

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60804A (ja) * 1983-06-20 1985-01-05 Poritetsukusu:Kk ウルトラフイルタ装置
JPS6188872A (ja) * 1984-10-09 1986-05-07 Snow Brand Milk Prod Co Ltd 高濃度連続培養方法及び装置
US4885087A (en) * 1986-11-26 1989-12-05 Kopf Henry B Apparatus for mass transfer involving biological/pharmaceutical media
US5030346A (en) * 1988-01-15 1991-07-09 Henry Filters, Inc. Pump for filtration system
US5401650A (en) * 1990-10-24 1995-03-28 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of biologically active α-galactosidase A
JPH0531335A (ja) * 1991-07-30 1993-02-09 Toto Ltd 分離膜洗浄方法
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
JP3547715B2 (ja) * 2001-03-06 2004-07-28 榮 新垣 ベクター精製用装置及び方法
ES2755552T3 (es) * 2002-06-19 2020-04-22 Northwest Biotherapeutics Inc Dispositivos de filtración de flujo tangencial y métodos para el enriquecimiento de leucocitos
US7875448B2 (en) 2004-01-12 2011-01-25 Single Use Brx, Llc Bioreactor systems and disposable bioreactor
PL1720972T3 (pl) * 2004-03-05 2014-06-30 Dpx Holdings Bv Proces hodowli komórek poprzez ciągłą perfuzję i zmienny przepływ styczny
EP1963367A4 (en) * 2005-12-06 2009-05-13 Amgen Inc POLISHING STEPS IN MULTI-STAGE PROTEIN PURIFICATION TREATMENTS
GB0606144D0 (en) * 2006-03-28 2006-05-10 Ge Healthcare Bio Sciences Ab Automated crossflow filtration method and system
AU2007272054C1 (en) * 2006-07-14 2014-08-28 Dpx Holdings B.V. Improved process for the culturing of cells
FR2925923B1 (fr) * 2007-12-28 2009-12-18 Michelin Soc Tech Procede et dispositif de fabrication d'un cable a deux couches du type gomme in situ
JP5003614B2 (ja) * 2008-07-03 2012-08-15 株式会社日立プラントテクノロジー 生体細胞の分離方法及び培養装置
US9446354B2 (en) * 2010-08-25 2016-09-20 Repligen Corporation Device, system and process for modification or concentration of cell-depleted fluid
JP5923529B2 (ja) * 2011-03-18 2016-05-24 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 細胞培養用可撓性バッグ
KR102059895B1 (ko) * 2012-02-20 2019-12-27 바이엘 악티엔게젤샤프트 세포를 유지하고 재순환시키기 위한 단방향 분리기
JP6321661B2 (ja) * 2012-09-27 2018-05-09 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ タンジェンシャルフロー灌流システム及び方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6022742A (en) * 1986-11-26 2000-02-08 Kopf; Henry B. Culture device and method
WO2013109520A1 (en) * 2012-01-18 2013-07-25 Bayer Healthcare Llc Perfusion bioreactor systems and methods of operating the same

Also Published As

Publication number Publication date
BR122021001466B1 (pt) 2021-10-13
AU2024219335A1 (en) 2024-09-19
EP3047013A1 (en) 2016-07-27
HUE055888T2 (hu) 2022-01-28
JP2020124204A (ja) 2020-08-20
EP3047013B1 (en) 2021-08-18
MX2021007792A (es) 2021-08-11
KR20160055866A (ko) 2016-05-18
MX2016003440A (es) 2016-11-11
EA201690602A1 (ru) 2016-07-29
SG11201601899TA (en) 2016-04-28
US20150158907A1 (en) 2015-06-11
JP2022050549A (ja) 2022-03-30
HK1222672A1 (zh) 2017-07-07
KR20230142653A (ko) 2023-10-11
CN113444620B (zh) 2024-03-29
IL244552B (en) 2020-04-30
CA2924332C (en) 2023-03-14
CA2924332A1 (en) 2015-03-19
JP7685543B2 (ja) 2025-05-29
KR102369211B1 (ko) 2022-03-03
AU2020202568A1 (en) 2020-05-07
ES2891728T3 (es) 2022-01-31
AU2014318420B2 (en) 2020-01-16
DK3047013T3 (da) 2021-11-15
EA031652B1 (ru) 2019-02-28
CY1124684T1 (el) 2022-07-22
KR102749545B1 (ko) 2025-01-07
SG10201803601SA (en) 2018-06-28
JP2016530893A (ja) 2016-10-06
MX384020B (es) 2025-03-14
CN105722967A (zh) 2016-06-29
EA201892084A1 (ru) 2019-02-28
JP7227406B2 (ja) 2023-02-21
BR112016005545B1 (pt) 2021-06-01
JP2023058588A (ja) 2023-04-25
JP7008746B2 (ja) 2022-01-25
KR20220031937A (ko) 2022-03-14
CN113444620A (zh) 2021-09-28
JP6925127B2 (ja) 2021-08-25
AU2020202568B2 (en) 2022-06-02
AU2022221495A1 (en) 2022-09-22
US20210269477A1 (en) 2021-09-02
IL244552A0 (en) 2016-04-21
AU2014318420A1 (en) 2016-04-14
EA035665B1 (ru) 2020-07-23
WO2015039115A1 (en) 2015-03-19

Similar Documents

Publication Publication Date Title
CN105722967B (zh) 用于处理细胞培养物的方法和系统
JP2022120031A (ja) シードトレイン法およびその使用
TWI767930B (zh) 處理包括重組治療性蛋白之流體的方法及其用途
CN108114508B (zh) 无菌层析树脂及其在制造工艺中的用途
US20150203529A1 (en) Sterile chromatography and manufacturing processes
RU2713126C2 (ru) Способ предотвращения контаминации системы
HK40057516A (en) Methods and systems for processing a cell culture
HK40057516B (zh) 用於处理细胞培养物的方法和系统
HK1222672B (en) Methods and systems for processing a cell culture
RU2786997C2 (ru) Процессы культивирования в системе посевных ферментеров и способы их применения

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant