CN105661109A - Special multi-vitamin premix feed for grass carp liver care - Google Patents

Special multi-vitamin premix feed for grass carp liver care Download PDF

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Publication number
CN105661109A
CN105661109A CN201610108212.0A CN201610108212A CN105661109A CN 105661109 A CN105661109 A CN 105661109A CN 201610108212 A CN201610108212 A CN 201610108212A CN 105661109 A CN105661109 A CN 105661109A
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vitamin
ctenopharyngodon idellus
premix material
hepatoprotective
multidimensional premix
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CN105661109B (en
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甄恕綦
姜瑞丽
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GUANGDONG HAID GROUP Co.,Ltd.
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GUANGZHOU HINTER BIOTECHNOLOGY CO Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Fodder In General (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention provides special multi-vitamin premix feed for grass carp liver care. The feed takes vitamins as a main component, and traditional Chinese herbal medicine is compounded for synergy. Reasonable compounding is carried out on the vitamins for solving the problem of hepatic and gall diseases of grass carps caused by long-term feed feeding during cultivation, and proved by experiments, by the premix feed, the morbidity of hepatic and gall diseases of the grass carps can be remarkably reduced, the survival rate in the cultivation is improved, and the income for the cultivation is increased.

Description

A kind of special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective
[technical field]
The present invention relates to a kind of Ctenopharyngodon idellus premix material, particularly relate to a kind of special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective, belong to feed additive field.
[background technology]
Ctenopharyngodon idellus (Ctenopharyngodonidellus) belongs to Cypriniformes, Cyprinidae, Leuciscinae, Ctenopharyngodon idellus belongs to, and is a kind of Fish being Major Foods with pasture and water in nature. This kind of Fish are one of China famous " four large Chinese carps ", and in China at present with in large fresh-water fishes of mixed feed cultivation, its yield is the highest, economic benefit highly significant. The water and soil resources that can be used for cultivating along with China constantly run off and the market continuous expansion to Ctenopharyngodon idellus demand, and the cultivation scale of Ctenopharyngodon idellus and cultivation density continue to increase. Although Ctenopharyngodon idellus is typical herbivorous fishes, but owing to mixed feed nutrient concentration is high, and feeding volume is big, Growth of Grass Carps Ctenopharyngodon Idellus is rapid, and current China is area intensive culture Ctenopharyngodon idellus a lot, mixed feed is adopted to throw something and feed completely, also Just because of this, it is easy to increase the weight of Ctenopharyngodon idellus liver and gall load, in this case, control the sickness rate of Ctenopharyngodon idellus, the economic benefit of the quality and increase Ctenopharyngodon idellus cultivation that ensure Ctenopharyngodon idellus is most important. Liver and gall diseases be Ctenopharyngodon idellus cultivation in common multiple disease, because the incubation period of this disease is long, and at the initial stage of a disease Ctenopharyngodon idellus disease symptom performance inconspicuous, not easily discovered by raiser, therefore once this disease is broken out on a large scale, the state of an illness is difficult to control to, and brings serious economic loss to raiser.
[summary of the invention]
For above-mentioned problems of the prior art, it is an object of the invention to provide a kind of easy to use, cheap, special multidimensional premix material of effect significant Ctenopharyngodon idellus hepatoprotective, thus effectively solving the liver and gall diseases problem caused in Ctenopharyngodon idellus breeding process because of mixed feed of throwing something and feeding.
To achieve these goals, the present invention is by the following technical solutions:
A kind of special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective, including vitamin A palmitate, vitamin D3, natural Vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, nicotinic acid, D-VB5 calcium, Bio, inositol and VBT.
Preferably, the special multidimensional premix material of above-mentioned Ctenopharyngodon idellus hepatoprotective is also mixed with Chinese herbal medicine, and described Chinese herbal medicine includes Semen Persicae, Rhizoma Curcumae Longae, Herba Cirsii, Fructus Hordei Germinatus and Rhizoma Cimicifugae.
Further, the special multidimensional premix material of above-mentioned Ctenopharyngodon idellus hepatoprotective also includes carrier, and described carrier is any one or more in Testa Tritici, defatted rice bran, powdered rice hulls, maize cob meal, Zein powder, Maifanitum.
Preferably, in above-mentioned multidimensional premix material, by percentage to the quality, including vitamin A palmitate 12-15%, vitamin D35-7%, natural Vitamin E 8-12%, vitamin K30.3-1%, vitamin B10.5-1%, vitamin B22-3%, vitamin B60.5-1.5%, vitamin B120.1-0.2%, folic acid 0.2-0.4%, nicotinic acid 2-5%, D-VB5 calcium 3.5-5%, Bio 0.1-0.2%, inositol 3-6%, VBT 4-7%, Semen Persicae 5-7%, Rhizoma Curcumae Longae 8-13%, Herba Cirsii 5-8%, Fructus Hordei Germinatus 3-6%, Rhizoma Cimicifugae 4-5%, carrier 14-17%.
Preferably, in above-mentioned multidimensional premix material, by percentage to the quality, including vitamin A palmitate 12%, vitamin D36%, natural Vitamin E 11%, vitamin K30.5%, vitamin B11%, vitamin B23%, vitamin B60.5%, vitamin B120.2%, folic acid 0.3%, nicotinic acid 3%, D-VB5 calcium 3.5%, Bio 0.2%, inositol 4%, VBT 5%, Semen Persicae 5%, Rhizoma Curcumae Longae 12%, Herba Cirsii 7%, Fructus Hordei Germinatus 4%, Rhizoma Cimicifugae 5%, carrier 16.8%.
Above-mentioned multidimensional premix material adding proportion in feed of grass carp is preferably 0.5%.
It is a further object of the present invention to provide a kind of method preparing the special multidimensional premix material of above-mentioned Ctenopharyngodon idellus hepatoprotective: pulverized through super micron mill by each raw material, all through the screen cloth of 300 orders, after mix homogeneously, to obtain final product.
Compared with prior art, there is advantages that
(1) presently commercially available general Ctenopharyngodon idellus multidimensional premix material can only meet Ctenopharyngodon idellus vitamin basic nutrition demand, only play prevention avitaminosis effect, do not possess liver-protecting function, vitamin premix of the present invention with vitamin for main component, composite Chinese herbal medicine potentiation, vitamin is carried out rationally composite by the liver and gall diseases problem caused because of feedstuff of throwing something and feeding for a long time in breeding process for Ctenopharyngodon idellus, tests prove that, Ctenopharyngodon idellus liver and gall sickness rate can be significantly reduced, improve cultivation survival rate, increase cultivation income.
(2) compared with other feed additives, Chinese herbal medicine has widely distributed as feed additive use, toxic and side effects is little, without hormone, not easily to features such as environments, the present invention selects common Chinese herbal medicine and vitamin composite, and selected vitamin is also the convenient source adopting prior art, raw material sources are convenient, and Semen Persicae, Rhizoma Curcumae Longae, Herba Cirsii, Rhizoma Cimicifugae have effect of blood circulation promoting and blood stasis dispelling, circulation of qi promoting, heat-clearing and toxic substances removing more; Containing multiple enzyme and vitamin B in Fructus Hordei Germinatus, be conducive to improving nutrition conversion ratio, alleviate the burden of Ctenopharyngodon idellus liver and gall, above-mentioned raw materials is all within " feed additive kind catalogue " and " feedstuff catalogue " that the Ministry of Agriculture promulgates, use safety, coordinate above-mentioned multivitamin, the function of its hepatoprotective can be strengthened.
(3) proportioning of the present invention is simple, preparation method is simple, with low cost, easy to use, be suitable to promote.
[detailed description of the invention]
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this. When not specified (NS), reagent, equipment and the method that the present invention adopts is the conventional commercial reagent of the art, equipment and conventional use of method.
Embodiment 1
Weigh vitamin A palmitate 15kg, vitamin D35kg, natural Vitamin E 8kg, vitamin K31kg, vitamin B10.5kg, vitamin B22kg, vitamin B61.5kg, vitamin B120.1kg, folic acid 0.2kg, nicotinic acid 2kg, D-VB5 calcium 4.6kg, Bio 0.1kg, inositol 6kg, VBT 7kg, Semen Persicae 7kg, Rhizoma Curcumae Longae 8kg, Herba Cirsii 5kg, Fructus Hordei Germinatus 6kg, Rhizoma Cimicifugae 4kg, Testa Tritici 17kg. Pulverize through super micron mill, all through the screen cloth of 300 orders, mix homogeneously.
Embodiment 2
Weigh vitamin A palmitate 13kg, vitamin D36kg, natural Vitamin E 12kg, vitamin K30.3kg, vitamin B10.5kg, vitamin B22.5kg, vitamin B61kg, vitamin B120.15kg, folic acid 0.4kg, nicotinic acid 5kg, D-VB5 calcium 5kg, Bio 0.15kg, inositol 3kg, VBT 4kg, Semen Persicae 6kg, Rhizoma Curcumae Longae 11kg, Herba Cirsii 8kg, Fructus Hordei Germinatus 3kg, Rhizoma Cimicifugae 5kg, defatted rice bran 7kg and powdered rice hulls 7kg.Pulverize through super micron mill, all through the screen cloth of 300 orders, mix homogeneously.
Embodiment 3
Weigh vitamin A palmitate 12kg, vitamin D36kg, natural Vitamin E 11kg, vitamin K30.5kg, vitamin B11kg, vitamin B23kg, vitamin B60.5kg, vitamin B120.2kg, folic acid 0.3kg, nicotinic acid 3kg, D-VB5 calcium 3.5kg, Bio 0.2kg, inositol 4kg, VBT 5kg, Semen Persicae 5kg, Rhizoma Curcumae Longae 12kg, Herba Cirsii 7kg, Fructus Hordei Germinatus 4kg, Rhizoma Cimicifugae 5kg, maize cob meal 16.8kg. Pulverize through super micron mill, all through the screen cloth of 300 orders, mix homogeneously.
Embodiment 4
Weigh vitamin A palmitate 14kg, vitamin D37kg, natural Vitamin E 9kg, vitamin K30.8kg, vitamin B10.8kg, vitamin B22kg, vitamin B60.5kg, vitamin B120.1kg, folic acid 0.2kg, nicotinic acid 4kg, D-VB5 calcium 3.5kg, Bio 0.1kg, inositol 5kg, VBT 6kg, Semen Persicae 5kg, Rhizoma Curcumae Longae 13kg, Herba Cirsii 6kg, Fructus Hordei Germinatus 5kg, Rhizoma Cimicifugae 4kg, Zein powder 10kg and Maifanitum 4kg. Pulverize through super micron mill, all through the screen cloth of 300 orders, mix homogeneously.
Compliance test result is tested:
The special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective of the present invention, has hepatoprotective effect, it is possible to reduces Ctenopharyngodon idellus liver and gall sickness rate, is illustrated below in conjunction with concrete application case.
Choose the cultivating pool of five raisers in Dongsheng Town, Zhongshan City, Guangdong Province as the test pool. Number consecutively is 1#, 2#, 3#, 4# and 5#. Five raisers are irrespective of size Ctenopharyngodon idellus raiser, put Seedling around, put Seedling specification unanimously, and breeding density is suitable, and the daily cultivation custom of five raisers is close. Wherein 1# tests pond area 11 mu, and Ctenopharyngodon idellus is 5500 tail about, and 2# tests pond area 9.8 mu, and Ctenopharyngodon idellus is 5000 tail about, 3# pond area 10 mu, and Ctenopharyngodon idellus is 5500 tail about, 4# pond area 10.4 mu, and Ctenopharyngodon idellus about 5000 tail, 5# pond area 10.7 mu, Ctenopharyngodon idellus is 5300 tail about.
Before on-test, five cultivating pools were respectively randomly selected Ctenopharyngodon idellus 15 tail, carry out serological index mensuration, anatomic observation liver situation, and carry out observing judgement to liver tissue slices, find that the Ctenopharyngodon idellus in five ponds all has a slight liver and gallbladder syndrome of suffering from, and the average extent of five pond Ctenopharyngodon idellus is suitable. Throwing something and feeding by the setting scheme of throwing something and feeding, every day two eats, and is satiated with food and throws something and feeds, terminating test after 1 month, after off-test, five groups of tests, pond is each fishes for Ctenopharyngodon idellus 20 tail at random, carry out serological index mensuration anatomic observation liver morphology, and carry out liver tissue slices observing judgement.
Described set the scheme of throwing something and feeding as: 1# tests pond: embodiment 1 group (the multidimensional premix material that basic components feedstuff+0.5% embodiment 1 without multidimensional premix material of throwing something and feeding produces), 2# tests pond: embodiment 2 groups (the multidimensional premix material that basic components feedstuff+0.5% embodiment 2 without multidimensional premix material of throwing something and feeding produces), 3# tests pond: embodiment 3 groups (the multidimensional premix material that basic components feedstuff+0.5% embodiment 3 without multidimensional premix material of throwing something and feeding produces), 4# tests pond: embodiment 4 groups (the multidimensional premix material that basic components feedstuff+0.5% embodiment 4 without multidimensional premix material of throwing something and feeding produces), 5# tests pond: matched group (mixed feed that basic components of throwing something and feeding produces). wherein, basic components, in air dry matter, including rapeseed cake 40 parts, Semen Tritici aestivi 20 parts, rice bran meal 15 parts, DDGS10 part, bean cake 5 parts, fish flour 2 parts, meat meal tankage 2 parts, dalcium biphosphate 2 parts, Ctenopharyngodon idellus multidimensional premix material 0.5 part, Ctenopharyngodon idellus many ore deposits premix material 1.5 parts, 1 part of Oleum Glycines, bentonite 1 part, described Ctenopharyngodon idellus multidimensional premix material and the premix material of Ctenopharyngodon idellus many ore deposits are commercial goods.
Carrying out serological index method for measuring is: Ctenopharyngodon idellus is carried out tail venous blood sampling, after its blood coagulation, uses 3000 revs/min of centrifuge centrifugal 10 minutes, prepare serum under 4 DEG C of conditions. Japan's Sysmex automatic clinical chemistry analyzer (Chemix-800) is used to measure triglyceride (TG), glutamate pyruvate transaminase (ALT) and glutamic oxaloacetic transaminase, GOT (AST). Use kit measurement lactic acid dehydrogenase (LDH), malonaldehyde (MDA). Its measurement result is as shown in table 1:
Grass Carp Serum index determining result respectively organized by table 1
Carry out liver tissue slices observing the method that judges as: take grass carp liver tissue, 3 hours are fixed respectively with BouinShi fixative, after scrubbed, dehydration, transparent, infiltration and embedding, it is cut into the thin slice that thickness is 5um, dye with HE staining after paster, then through dehydration and transparent after use neutral gum mounting, it is placed in basis of microscopic observation, if observing liver cell nuclear off-center, between hepatocyte, boundary is unclear, part of hepatocytes form disappears, and graininess degeneration, vacuolar degeneration occurs, it is determined that for hepatic lesions; If observing hepatocyte marshalling, without degeneration, necrosis and inflammatory cell infiltration, rope is centered by central vein, it is arranged radially to surrounding, rope hole ratio is normal, hepatocyte boundary clear, and nucleus is positioned at cell central authorities, portal area has no proliferation of fibrous tissue, then judge hepatic lesions. Its statistical result is as shown in table 2:
Liver of Ctenopharyngodon Idellus pathological changes situation respectively organized by table 2
Group Dissect fish tail number (tail) Hepatic lesions fish tail number (tail) Prevalence (%)
Matched group 20 19 95
Embodiment 1 group 20 1 5
Embodiment 2 groups 20 1 5
Embodiment 3 groups 20 0 0
Embodiment 4 groups 20 1 5
From table 1 and table 2; compared with matched group; in the Grass Carp Serum of embodiment 1-4 group, transaminase and lactic acid dehydrogenase activity all significantly reduce; and multidimensional premix material of the present invention can substantially reduce the content of triglyceride and malonaldehyde; the protective rate of Liver of Ctenopharyngodon Idellus is reached more than 90%, and protected effect is notable.
The above; it is only the preferably specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those familiar with the art is in the technical scope that the invention discloses; it is equal to replacement according to technical scheme and design thereof or is changed, all should be encompassed in protection scope of the present invention.

Claims (7)

1. the special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective, it is characterised in that include vitamin A palmitate, vitamin D3, natural Vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, nicotinic acid, D-VB5 calcium, Bio, inositol and VBT.
2. the special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective according to claim 1, it is characterised in that being also mixed with Chinese herbal medicine in described multidimensional premix material, described Chinese herbal medicine includes Semen Persicae, Rhizoma Curcumae Longae, Herba Cirsii, Fructus Hordei Germinatus and Rhizoma Cimicifugae.
3. the special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective according to claim 2, it is characterized in that, described multidimensional premix material also includes carrier, and described carrier is any one or more in Testa Tritici, defatted rice bran, powdered rice hulls, maize cob meal, Zein powder, Maifanitum.
4. the special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective according to claim 3, it is characterised in that by percentage to the quality, including vitamin A palmitate 12-15%, vitamin D35-7%, natural Vitamin E 8-12%, vitamin K30.3-1%, vitamin B10.5-1%, vitamin B22-3%, vitamin B60.5-1.5%, vitamin B120.1-0.2%, folic acid 0.2-0.4%, nicotinic acid 2-5%, D-VB5 calcium 3.5-5%, Bio 0.1-0.2%, inositol 3-6%, VBT 4-7%, Semen Persicae 5-7%, Rhizoma Curcumae Longae 8-13%, Herba Cirsii 5-8%, Fructus Hordei Germinatus 3-6%, Rhizoma Cimicifugae 4-5%, carrier 14-17%.
5. the special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective according to claim 4, it is characterised in that by percentage to the quality, including vitamin A palmitate 12%, vitamin D36%, natural Vitamin E 11%, vitamin K30.5%, vitamin B11%, vitamin B23%, vitamin B60.5%, vitamin B120.2%, folic acid 0.3%, nicotinic acid 3%, D-VB5 calcium 3.5%, Bio 0.2%, inositol 4%, VBT 5%, Semen Persicae 5%, Rhizoma Curcumae Longae 12%, Herba Cirsii 7%, Fructus Hordei Germinatus 4%, Rhizoma Cimicifugae 5%, carrier 16.8%.
6. the special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective according to any one of claim 1-5, it is characterised in that the adding proportion in feed of grass carp is 0.5%.
7. the method for the special multidimensional premix material of Ctenopharyngodon idellus hepatoprotective that one kind is prepared according to any one of claim 1-5, it is characterised in that each raw material is pulverized through super micron mill, all through the screen cloth of 300 orders, after mix homogeneously, to obtain final product.
CN201610108212.0A 2016-02-26 2016-02-26 A kind of dedicated multidimensional premix of grass carp protect liver Active CN105661109B (en)

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CN106212899A (en) * 2016-07-25 2016-12-14 四川新美生物科技有限公司 A kind of preprocess method of microelement feed addictive
CN106962692A (en) * 2017-05-16 2017-07-21 清远海贝生物技术有限公司 A kind of premix for repairing the green liver pathological changes of grass carp and its preparation method and application
CN107410758A (en) * 2017-05-16 2017-12-01 清远海贝生物技术有限公司 A kind of premix for repairing grass carp Huang liver pathological changes and its preparation method and application

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106212899A (en) * 2016-07-25 2016-12-14 四川新美生物科技有限公司 A kind of preprocess method of microelement feed addictive
CN106962692A (en) * 2017-05-16 2017-07-21 清远海贝生物技术有限公司 A kind of premix for repairing the green liver pathological changes of grass carp and its preparation method and application
CN107410758A (en) * 2017-05-16 2017-12-01 清远海贝生物技术有限公司 A kind of premix for repairing grass carp Huang liver pathological changes and its preparation method and application
CN107410758B (en) * 2017-05-16 2019-10-18 清远海贝生物技术有限公司 A kind of premix and its preparation method and application for repairing grass carp Huang liver pathological changes
CN106962692B (en) * 2017-05-16 2019-11-15 广东海大集团股份有限公司 A kind of premix and its preparation method and application for repairing the green liver pathological changes of grass carp

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Patentee after: GUANGDONG HAID GROUP Co.,Ltd.

Address before: No. 56, Xingui 2nd Road, Guangzhou hi tech Industrial Development Zone, Guangzhou, Guangdong 510530

Patentee before: GUANGDONG HINTER BIO-TECHNOLOGY GROUP Co.,Ltd.