CN105660639B - The microbicide compositions and its application of oligosaccharide element and jinggangmycin A - Google Patents
The microbicide compositions and its application of oligosaccharide element and jinggangmycin A Download PDFInfo
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- CN105660639B CN105660639B CN201511033034.1A CN201511033034A CN105660639B CN 105660639 B CN105660639 B CN 105660639B CN 201511033034 A CN201511033034 A CN 201511033034A CN 105660639 B CN105660639 B CN 105660639B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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Abstract
The present invention relates to a kind of agricultural bactericidal composition and its applications.The composition is using oligosaccharide element and jinggangmycin A as main active, and wherein the mass ratio of oligosaccharide element and jinggangmycin A is 1: 1~1: 30.Indoor and field trial shows that the composition has good control effect to rice green smut, in addition, also have many advantages, such as lasting period length, nontoxicity, noresidue, to person poultry harmless, drug cost it is low, it is free from environmental pollution, reuse and will not generate specific drug resistance.
Description
First, technical field
The present invention relates to a kind of agriculture chemical compounding combination and application thereof, using the bactericide compounded technology of two kinds of different types come
Rice green smut is prevented, belongs to the new application of fungicide and its application technology invention.
2nd, technical background
Oligosaccharide element is botanical pesticide, is a kind of carbohydrate of complexity, is special oligosaccharide, it is in plant
It is interior as signaling molecule coordinate plant growth, development and survival ability in the environment.Oligosaccharide element, that is, plant or parasitic animal and plant water
Solution enzyme leads to anaphylactoid activity excitation of host plant, induction from what plant or disease fungus cell wall polysaccharides decomposited
Plant synthesis, accumulation antitoxin.
Jinggangmycin A is the farm antibiotics that a kind of actinomyces generate, and has stronger absorbability, is easily inhaled by somatic cells
It receives and is conducted rapidly in it, interfered and inhibit somatic cells growth and development, there is good therapeutic effect.Jinggangmycin A
To people and animals' low toxicity, to birds fish safety.It is big to be mainly used for prevention rice green smut, rice sheath blight disease, wheat sharp eyespot, corn
Helminthosporium maydis etc., for many years large-scale field test results prove that its preventive effect is high, nuisanceless, pollution-free, be a kind of ideal biology
Pesticide.
False smut (Ustilaginoiden virens (Cooke) Tak) belongs to Ascomycetes Clavicipitaceae, is on rice
A kind of common fungal disease, popular in recent years to occur, harm aggravates year by year.The disease mainly occurs in Rice Heading blooming stage,
Part grain on fringe is endangered, is commonly called as green powder disease, black ball disease and pseudo- smut.False smut can cause rice rate of immatured grain to increase, thousand
Decline again, drop to rice quality.In addition the disease can also generate the toxin being harmful to people and animals, and people's health is formed and is endangered.Cause
The generation of this control false smut, it is most important to Rice Production and grain security.At present, Agro-chemicals control is still control rice
The main method of bent disease, however false smut has resistance to organic sulphur preparation, to more bacterium copper, DT, the efficiently medicament such as can to kill easy
Generate poisoning.Therefore, according to the pests occurrence rule of false smut and prevention situation, it is badly in need of developing efficient, wide spectrum, low toxicity, safety new
Type fungicide or compounding agent prevent false smut instead of above-mentioned medicament.
3rd, invention content
Technical problem is solved present invention aims at the new type bactericide compounded formula of research and development prevention rice green smut
Technical problem underlying be to prevent the high significant effect of false smut, bactericidal activity, safer environmental protection and other medicaments can be administered resist
Pharmacological property and drug injury problem are provided for improvement false smut disease and are used in combination with the good bactericide compounded formula of different role mode compatibility
It is put into practice in Instructing manufacture.
Selection of technical scheme is to false smut bactericidal activity height, two environmentally safe, less toxic with different role mode
First fungicide oligosaccharide element and jinggangmycin A carry out Toxicity Determination and compounded formula screening, obtain indoor performance synergy and make
Formula chooses synergistic effect and significantly matches formula for field control effectiveness test.
The advantageous effect present invention is also easy to produce anti-medicine for single bactericide prevention false smut is used for a long time in existing production
Property or poisoning, lead to problems such as medication fail or decline, with botanical pesticide oligosaccharide element and farm antibiotics jinggangmycin A two
Member compounding by Toxicity Determination, as a result obtains oligosaccharide element: the composite bactericide of jinggangmycin A 1: 3~15 is to rice song
The indoor virulence activity of disease is high, there is apparent synergistic effect, wherein when being 1: 12 with oligosaccharide element and jinggangmycin A proportioning, increases
Effect effect is maximum, and the compounding agent of this proportioning also shows more significant synergistic effect in field efficacy experiment.With single fungicide
Prevention false smut, which is compared, to be had the advantages that.
1. the invention belongs to the formula of binary built fungicide, there is compatibility, improve bactericidal activity, it is bent to prevention rice
Disease has more significant synergistic effect, reduces the dosage of single bactericide, saves Pesticide use cost, reduces environment
Pollution and pesticide residue.
2. the formula is the compounding of the binary fungicide of different role mode, there is efficient, wide spectrum, lasting, increasing
The action site to ustilaginoidea virens is added, and effect is significantly inhibited to false smut drug resistance occurrence and development.
3. the bactericidal effect of the formula significantly improves, the effective Instructing manufacture application of energy reduces ustilaginoidea virens and rice is given birth to
Cause harm caused by production, improve the yield and quality of rice.
4th, specific embodiment and effect
(1) formula indoor screens
The present invention for research object, carries out indoor compound drug with ustilaginoidea virens (Ustilaginoidea virens)
Recipe determination.Reference《The 8th part of pesticide indoor biometrics experiment criterion fungicide：Prevent rice blast experiment pot-culture method》Mark
Quasi- method carries out.Ustilaginoidea virens U-22 bacterial strains preculture 9d on PSA culture mediums, in the bacterium colony to PS culture solutions of shifter edge,
28 DEG C, shaken cultivation 14d under conditions of 120r/min, fully smash mycelia with refiner, will contain broken section of mycelia and mitogenetic
The mixed liquor of spore is as inoculum；Excellent 988 seeds of town Xian 85% strong chlorine oil, 500 times of liquid seed soaking 12h, then fully rushed with clear water
Seedling-cultivation plate is seeded in after washing, according to a conventional method seedling.Rice shoot tri-leaf period is moved in plastic cup, every glass of 3~4 young plants of shifting, conventional tube
Reason to rice booting is begun the phase, and every glass of stay growth more consistent 7~10 plants are made for examination plant；64% jinggangmycin A active compound,
90.2% oligosaccharide element active compound is dissolved into the mother liquor of 10000 μ g/ml with distilled water respectively, and adds in 0.5% in liquid mother liquor
Tween-20 makees surfactant to increase the adhesion on plant blade face, then dilutes 2 confessions with the aqueous solution of 0.1% Tween-80
The sample of the mother liquor of reagent agent and 11 mixtures is prepared the liquid of various concentration by serial dilutions；Every 4 glasss of rice (begin by booting
Phase) be placed in the medicament of PWT-510 type spray towers quantification spraying various concentration, after spray after blade liquid without it is apparent it is water stain after will
Plastic cup moves on to 29~31 DEG C, cultivates in the growth room of 16h illumination.Each concentration handles 4 repetitions, often repeatedly 1 plastic cup, with
The processing of clear water is sprayed as control treatment.Each second of spray of 9d progress after handling spray for the first time；Each processing rice seedling is second
4d carries out the artificial infection of false smut after secondary spray.The inoculation method of false smut with reference to Mr. Zhang into wait (2003, rice in China section
Learn) method.Rice plant after inoculation, which is still moved in growth room, to be cultivated.Investigation method：After rice green smut illness is stablized, note
Rice curve grain number on the every fringe of record calculates average infected seed number/fringe that each processing often repeats.Control effect is calculated as follows：Prevention effect
Fruit %=[(control treatment be averaged infected seed number-chemicals treatment be averaged infected seed number)/control treatment be averaged infected seed number] × 100, use DPS
Software carries out data processing, calculates regression equation, EC5o, related coefficient.The synergy of mixture is measured according to the abundant methods of Sun Yun
(1960) co-toxicity coefficient (CTC) is calculated.The co-toxicity for judging medicament with CTC values acts on, and CTC≤80 show as antagonism；
80 ＜ CTC ＜ 120 show as summation action；CTC >=120 show as synergistic effect.
1 oligosaccharide element of table and jinggangmycin A different agents are with the virulence for comparing ustilaginoidea virens
Table 1 statistics indicate that, the oligosaccharide element of different ratio and jinggangmycin A are to the EC of false smut under the conditions of indoor living5o
It is different, between 80.3812~225.3733, matching when being 1: 3~15, there is co-toxicity coefficient (CTC) value synergy to make
With wherein when being 1: 12 with oligosaccharide element and jinggangmycin A proportioning, co-toxicity coefficient is maximum, illustrates the oligosaccharide under this proportioning
Element and the effect of jinggangmycin A compound synergic are most notable.
(2) embodiment is prepared
The present invention is further described with reference to specific embodiment, but does not limit the invention to this tool
Body embodiment.One skilled in the art would recognize that present invention encompasses the institutes that may include in Claims scope
There are alternative, improvement project and equivalent scheme.
Percentage composition is weight percentage in preparation proportioning.
Suspending agent
By deionized water, dispersant wetting agent according to formula rate, it is pumped into the configuration kettle for being equipped with high shear agitation machine,
Stirring is opened to being completely dissolved.Active compound is added in continue to shear half an hour.Other auxiliary materials are proportionally added in configuration kettle,
Continue shearing 45 minutes.It opens two-stage to be sanded, controls discharging speed, obtain suspending agent.
Embodiment：13% oligosaccharide element jinggangmycin A suspending agent (active ingredient 1: 12)
Weigh 1.1% oligosaccharide element active compound (content 90.2%), 19.3% jinggangmycin A active compound (content 64%), 8% point
Powder T/36,4% wetting agent YUS-TXC, 6% antifreeze propylene glycol, 2% preservative butyl hydroxybenzoate, 0.2% thickener xanthan
Glue, 0.5% antifoaming agent SAG630,2% suspending agent aluminium-magnesium silicate, 56.9% deionized water.Slurry is made, is sanded, obtains
Product.
(3) field trial of rice green smut is prevented
Experiment is located at one peasant household rice field of Huai'an Hongze County Cha He towns Shi Tang villages, experimental plot for rice replanting for many years, and
Morbidity is serious over the years for false smut, investigates incidence, carries out field control effectiveness test.Experimental plot is divided into 18 cells, Mei Ge little
Area's area is 20m2, 5 processing of experiment setting：(1) 6% oligosaccharide element aqua 1.5g.a.i./mu；(2) 28% jinggangmycin As
Solvable pulvis 11.2g.a.i./mu；(3) 13% oligosaccharide element jinggangmycin A suspending agent 1.3g.a.i./mu (4) 13% is oligomeric
Sugared element jinggangmycin A suspending agent 2.6g.a.i./mu；(5) 13% oligosaccharide element jinggangmycin A suspending agent 5.2g.a.i./
Mu, and clear water control is set, each handle 3 repetitions, different disposal cell random distribution.When rice 7 days away from the cut phase or so
Start spray (spraying using Singapore's profit agriculture Jacto 16LITROS types sprayer, aperture ＜ 7mm, medicament does not stay raffinate), often
50 kilograms of spraying liquid of mu carries out second in 7 days after spraying for the first time and sprays.The investigation of preventive effect after second of dispenser 14 days into
Row, investigation method are per 5 points of samplings of cell, and every is investigated 10 clumps, totally 50 clumps, records total spike number and morbidity spike number, and by classification
Standard grading calculates disease index and control effect.Result of the test is shown in Table 2.
False smut grade scale：
0 grade：It is disease-free；
1 grade：Per fringe, there are one infected seeds；
2 grades：There is 2-4 infected seed per fringe；
3 grades：There is 5-7 infected seed per fringe；
4 grades：There is 8-10 infected seed per fringe.
5 grades：There are 10 or more infected seeds per fringe.
2 13% oligosaccharide element jinggangmycin A suspending agent of table is to the field control effect of rice green smut
(4) conclusion (of pressure testing)
Indoor virulence test shows that two kinds of oligosaccharide element, jinggangmycin A fungicide active ingredients have compatibility, oligosaccharide
Plain, jinggangmycin A compounding agent synergy ratio range is 1: 3~15, and the optimum compound proportion of the two is 1: 12.Field trial table
Bright, 13% oligosaccharide element jinggangmycin A suspending agent is significantly higher than the preventive effect of false smut anti-when jinggangmycin A is used alone
Effect.In terms of false smut is prevented, although the effect of oligosaccharide element single dose is also fine, use cost is very high.Therefore, it is real in production
It tramples middle oligosaccharide element to be used in combination with jinggangmycin A, not only economic but also efficient, environmental protection.
Claims (2)
1. application of the microbicide compositions of element containing oligosaccharide and jinggangmycin A in rice green smut is prevented;Described containing is low
The microbicide compositions of glycan element and jinggangmycin A, active ingredient are oligosaccharide element and jinggangmycin A, wherein oligosaccharide element
Mass ratio with jinggangmycin A is 1: 12~1: 15.
2. application according to claim 1, it is characterised in that the oligosaccharide element and the mass ratio of jinggangmycin A are 1
: 12, show synergistic effect.
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| CN201511033034.1A CN105660639B (en) | 2015-12-31 | 2015-12-31 | The microbicide compositions and its application of oligosaccharide element and jinggangmycin A |
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| CN201511033034.1A CN105660639B (en) | 2015-12-31 | 2015-12-31 | The microbicide compositions and its application of oligosaccharide element and jinggangmycin A |
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| CN1299588A (en) * | 1999-12-10 | 2001-06-20 | 广东原沣生物工程有限公司 | Application of oligosaccharide in biological pesticide |
| CN104430347A (en) * | 2014-10-28 | 2015-03-25 | 山东中大药业有限公司 | Compound emulsion in water of chitosan oligosaccharide and validamycin A |
| CN104322495A (en) * | 2014-10-28 | 2015-02-04 | 山东中大药业有限公司 | Wettable powder for preventing and controlling wheat sharp eyespot |
| CN104351180B (en) * | 2014-10-28 | 2016-08-24 | 青岛青知企业管理咨询有限公司 | A kind of micro-capsule suspension for preventing and treating rice green smut |
| CN104304249B (en) * | 2014-10-28 | 2016-08-17 | 青岛青知企业管理咨询有限公司 | A kind of for slow releasing agent preventing and treating wheat scab and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972428A (en) * | 2012-12-11 | 2013-03-20 | 北京大北农科技集团股份有限公司 | Method for controlling pests |
| CN103283725A (en) * | 2013-05-22 | 2013-09-11 | 江苏省绿盾植保农药实验有限公司 | Sterilization composition containing amino-oligosaccharin and validamycin and application of pesticide composition |
Non-Patent Citations (1)
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| 《0.4 % 低聚糖素(OS)水剂对几种植物病原真菌的抗菌活性及对水稻纹枯病的田间防效》;张斌;《农药》;20050630;全文 * |
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Inventor after: Zhang Aihua Inventor after: Kong Weibao Inventor after: Xu Congying Inventor before: Kong Weibao Inventor before: Xu Congying |