CN105660639A

CN105660639A - Bactericide composition containing oligosaccharins and validamycin A, and application thereof

Info

Publication number: CN105660639A
Application number: CN201511033034.1A
Authority: CN
Inventors: 孔维宝; 徐从英
Original assignee: Jiangsu Bangsheng Biological Technology Responsibility Co Ltd
Current assignee: Jiangsu Bangsheng Biological Technology Responsibility Co Ltd
Priority date: 2015-12-31
Filing date: 2015-12-31
Publication date: 2016-06-15
Anticipated expiration: 2035-12-31
Also published as: CN105660639B

Abstract

The invention relates to an agricultural sterilization composition and an application thereof. The composition adopts oligosaccharins and validamycin A as main active components, wherein a mass ratio of the oligosaccharins to validamycin A is 1:1-1:30. Indoor and field test shows that the composition has a good prevention and control effect on rice false smut, and also has the advantages of long lasting period, non-toxicity, no residuals, harmlessness to humans and animals, low medication cost, no pollution to environment, and no generation of specific pesticide resistance after repeated use.

Description

The microbicide compositions of oligosaccharide element and jinggangmycin A and application thereof

One, technical field

The present invention relates to a kind of agriculture chemical compounding combination and application thereof, adopt two kinds of dissimilar bactericide compounded technology to prevent and treat rice green smut, belong to new application and the application technology invention thereof of antibacterial.

Two, technical background

Oligosaccharide element is botanical pesticide, is the carbohydrate of a kind of complexity, is special oligosaccharide, it in plant as signaling molecule coordinate plant growth, growth and survival ability in the environment. Oligosaccharide element and plant or parasite hydrolytic enzyme cause anaphylactoid activity excitation of host plant, induction plant synthesis, accumulation antitoxin from what plant or pathogenic fungi cell wall polysaccharides decomposed out.

Jinggangmycin A is the agricultural antibiotic that a kind of actinomycetes produce, and has stronger interior absorption, is easily absorbed by somatic cells and within it conducts rapidly, disturbs and suppresses somatic cells g and D, having good therapeutical effect. Jinggangmycin A is to people and animals' low toxicity, to birds Fish safety. Being mainly used in preventing and treating rice green smut, rice sheath blight disease, wheat sharp eyespot, corn northern and southern leaf blight etc., large-scale field test results proves that its preventive effect is high, nuisanceless, pollution-free for many years, is a kind of desirably biological pesticide.

False smut (Ustilaginoidenvirens (Cooke) Tak) belongs to Ascomycetes Clavicipitaceae, is a kind of fungal disease common on Oryza sativa L., in recent years popular generation, and harm increases the weight of year by year. This disease is main to be occurred at Rice Heading blooming stage, and part grain on harm fringe is commonly called as Calomelas disease, the sick and pseudo-smut of black ball. False smut may result in the increase of Oryza sativa L. rate of immatured grain, mass of 1000 kernel declines, and drops to rice quality. Additionally this disease can also produce toxin that people and animals are harmful to, and healthy to people constitutes harm. Therefore the generation of false smut is controlled, Rice Production and grain security is most important. At present, Agro-chemicals control remains the main method controlling false smut, but false smut is to the existing resistance of organic sulfur preparation, to many bacterium copper, DT, the efficiently medicament such as can kill and is easily generated poisoning. Therefore, according to the pests occurrence rule of false smut and prevent and treat situation, it is badly in need of developing efficiently, wide spectrum, low toxicity, the new type bactericide of safety or built agent replace above-mentioned medicament to prevent and treat false smut.

Three, summary of the invention

Technical problem present invention aim at the new type bactericide compounded formula of research and development preventing and treating rice green smut, the technical problem underlying of its solution be prevent and treat that the effect of false smut is notable, bactericidal activity is high, safer environmental protection other medicament Drug resistance and drug injury problems can be administered, provide there is the good bactericide compounded formula of the different model of action compatibility and for Instructing manufacture practice for administering false smut disease.

Selection of technical scheme is high to false smut bactericidal activity, the binary antibacterial oligosaccharide with different model of action environmentally safe, low toxicity is plain and jinggangmycin A carries out Toxicity Determination and compounded formula screens, obtain the formula of indoor performance potentiation, choose the significant proportioning formula of potentiation for field control effectiveness test.

Beneficial effect the present invention is directed to the single bactericidal agent for preventing and treating false smut of life-time service in existing production and is easily generated Drug resistance or poisoning, cause that medication was lost efficacy or lower degradation problem, with botanical pesticide oligosaccharide element and agricultural antibiotic jinggangmycin A binary built, pass through Toxicity Determination, result draws oligosaccharide element: the composite bactericide of jinggangmycin A 1: 3～15 is high to the indoor virulence activity of false smut, there is obvious potentiation, wherein with oligosaccharide element and jinggangmycin A proportioning for 1: 12 time, potentiation is maximum, the built agent of this proportioning also shows more significant potentiation in field efficacy is tested. have the advantages that compared with single bactericidal agent for preventing and treating false smut.

1. the invention belongs to the formula of binary built antibacterial, there is the compatibility, improve bactericidal activity, to preventing and treating false smut, there is more significant potentiation, decrease the dosage of single antibacterial, save Pesticide use cost, reduce environmental pollution and pesticide residues.

2. this formula is the composite of the binary antibacterial of different model of action, has efficiently, wide spectrum, the advantage such as lasting, adds the action site to ustilaginoidea virens, and false smut Drug resistance is occurred and development significantly inhibits effect.

3. the bactericidal effect of this formula significantly improves, and can apply by effective Instructing manufacture, and what Rice Production was caused by minimizing ustilaginoidea virens causes harm, and improves the yield and quality of Oryza sativa L..

Four, specific embodiments and effect

(1) formula indoor screening

The present invention, with ustilaginoidea virens (Ustilaginoideavirens) for object of study, carries out the recipe determination of indoor compound drug. Standard method with reference to " pesticide indoor biometrics test rule antibacterial the 8th part: preventing and treating rice blast test pot-culture method " carries out. Ustilaginoidea virens U-22 bacterial strain preculture 9d in PSA culture medium, the bacterium colony of shifter edge is to PS culture fluid, 28 DEG C, 120r/min when shaken cultivation 14d, with refiner, mycelia is fully smashed, will containing broken section of mycelia and conidial mixed liquor as inoculum; Excellent 988 seeds of town Xian 500 times of immersion kind 12h of 85% strong chlorine oil, then it is seeded in seedling-cultivation plate after fully rinsing with clear water, seedling according to a conventional method. Rice shoot moves in plastic cup tri-leaf period, and every glass is moved 3～4 young plants, and Routine Management begins the phase to Oryza sativa L. booting, and every glass of 7～10 strains staying growth more consistent are made for examination plant; The 64% former medicine of jinggangmycin A, the 90.2% oligosaccharide former medicinal distilled water of element are dissolved into the mother solution of 10000 μ g/ml respectively, and in medicinal liquid mother solution, add 0.5% tween 20 make surfactant to increase tack on plant blade face, dilute the mother solution of 2 reagent agents and the sample of 11 mixtures again with the aqueous solution of 0.1% tween 80, prepare the medicinal liquid of variable concentrations by serial dilutions; Every 4 glasss of Oryza sativa L. (booting begin phase) are placed in the medicament of PWT-510 type spray tower quantification spraying variable concentrations, until blade medicinal liquid without plastic cup moving on to after substantially water stain 29～31 DEG C, cultivating in the growth room of 16h illumination after spray medicine. Each concentration processes 4 repetition, often 1 plastic cup of repetition, to spray the process of clear water as control treatment. After each process first time spray medicine, 9d carries out spraying medicine for the second time; Each rice seedling 4d after second time spray medicine that processes carries out the artificial vaccination of false smut. The method that the inoculation method of false smut becomes etc. (2003, rice in China science) with reference to Mr. Zhang. Postvaccinal rice plant still moves in growth room and cultivates. Investigation method: after rice green smut disease is stable, records rice curve grain number on every fringe, calculate average infected seed number/fringe that each process often repeats.It is calculated as follows prevention effect: prevention effect %=[(control treatment average infected seed number-chemicals treatment average infected seed number) the average infected seed number of/control treatment] × 100, carries out data process with DPS software, calculate regression equation, EC_5o, correlation coefficient. The synergy of mixture measures and calculates co-toxicity coefficient (CTC) according to the abundant method of Sun Yun (1960). Judge the co-toxicity effect of medicament with CTC value, CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action; CTC >=120 show as potentiation.

Table 1 oligosaccharide element and the jinggangmycin A different agents proportioning virulence to ustilaginoidea virens

Table 1 data show, when indoor living different ratio oligosaccharide element and the jinggangmycin A EC to false smut_5oDiffer, co-toxicity coefficient (CTC) value is between 80.3812～225.3733, proportioning has potentiation when being 1: 3～15, wherein with oligosaccharide element and jinggangmycin A proportioning for 1: 12 time, its co-toxicity coefficient is maximum, illustrates that the oligosaccharide element under this proportioning and jinggangmycin A compound synergic effect are the most notable.

(2) embodiment is prepared

Below in conjunction with specific embodiment, the present invention is further described, but does not limit the invention to this specific embodiment. One skilled in the art would recognize that and present invention encompasses all alternatives, improvement project and the equivalents potentially included in Claims scope.

In preparation proportioning, percentage composition is weight percentage.

Suspending agent

By deionized water, dispersant wetting agent according to formula proportion, pump in the configuration still being equipped with high shear agitation machine, open stirring to being completely dissolved. Add former medicine to continue to shear half an hour. Other assist material proportionally add in configuration still, continues to shear 45 minutes. Open two-stage sand milling, control discharging speed, obtain suspending agent.

Embodiment: 13% oligosaccharide element jinggangmycin A suspending agent (effective ingredient 1: 12)

Weigh the 1.1% oligosaccharide former medicine of element (content 90.2%), the 19.3% former medicine of jinggangmycin A (content 64%), 8% dispersant T/36,4% wetting agent YUS-TXC, 6% antifreeze propylene glycol, 2% preservative butoben, 0.2% thickening agent xanthan gum, 0.5% defoamer SAG630,2% suspending agent aluminium-magnesium silicate, 56.9% deionized water. Make slurry, carry out sand milling, obtain product.

(3) field test of rice green smut is prevented and treated

Test is located at peasant household of Shi Tang village one, Cha He town, Hongze County, Huai'an rice field, and experimental plot be Oryza sativa L. replanting ground for many years, and false smut falls ill seriously over the years, investigation incidence, carries out field control effectiveness test. Experimental plot is divided into 18 communities, and each plot area is 20m², test arranges 5 process: (1) 6% oligosaccharide element water preparation 1.5g.a.i./mu; (2) the 28% solvable powder 11.2g.a.i./mus of jinggangmycin A; (3) 13% oligosaccharide element jinggangmycin A suspending agent 1.3g.a.i./mu (4) 13% oligosaccharide element jinggangmycin A suspending agent 2.6g.a.i./mu; (5) 13% oligosaccharide element jinggangmycin A suspending agent 5.2g.a.i./mu, and set clear water comparison, each process 3 repetition, different disposal community random distribution. Start to spray medicine when Oryza sativa L. was from about 7 days cut phase and (use Singapore's profit agriculture Jacto16LITROS type aerosol apparatus spraying, aperture < 7mm, medicament does not stay residual liquid), every mu of spraying liquid 50 kilograms, within 7 days, carry out second time after first time spray medicine and spray medicine. The investigation of preventive effect carries out for 14 days after second time dispenser, and investigation method is the sampling of 5, every community, investigates 10 clumps at every, totally 50 clumps, records total spike number and morbidity spike number, and presses grade scale classification, calculates disease index and prevention effect.Result of the test is in Table 2.

False smut grade scale:

0 grade: anosis;

1 grade: every fringe has an infected seed;

2 grades: every fringe has 2-4 infected seed;

3 grades: every fringe has 5-7 infected seed;

4 grades: every fringe has 8-10 infected seed.

5 grades: every fringe has more than 10 infected seeds.

The table 213% oligosaccharide element jinggangmycin A suspending agent field control effect to rice green smut

(4) conclusion (of pressure testing)

Indoor virulence test shows that oligosaccharide element, two kinds of antibacterial effective ingredient of jinggangmycin A have the compatibility, and oligosaccharide is plain, the potentiation ratio range of jinggangmycin A built agent is 1: 3～15, and both optimum compound proportions are 1: 12. Field test shows, the preventive effect of false smut is significantly higher than preventive effect when jinggangmycin A is used alone by 13% oligosaccharide element jinggangmycin A suspending agent. In preventing and treating false smut, although the effect of oligosaccharide element single dose is also fine, but use cost is significantly high. Therefore, in production practices, oligosaccharide element is used in combination with jinggangmycin A, not only economy but also efficient, environmental protection.

Claims

1. containing a microbicide compositions for oligosaccharide element and jinggangmycin A, it is characterized in that: its active ingredient is oligosaccharide element and jinggangmycin A, and wherein the mass ratio of oligosaccharide element and jinggangmycin A is 1: 1～1: 30.

2. the microbicide compositions containing oligosaccharide element and jinggangmycin A according to claim 1, it is characterised in that the mass ratio of oligosaccharide element and jingganmycin is 1: 3～1: 15, shows potentiation.

3. according to claim 1 containing oligosaccharide element and the microbicide compositions of jinggangmycin A, it is characterised in that the dosage form that described oligosaccharide element and jinggangmycin A are processed on pesticide with known surfactant and auxiliary agent etc. to allow.

4. the microbicide compositions containing oligosaccharide element and jinggangmycin A according to claim 1 is used for preventing and treating rice green smut.

5. the microbicide compositions containing oligosaccharide element and jinggangmycin A according to claim 1, it is characterised in that oligosaccharide element and jinggangmycin A carry out composite according to 1: 12, and the preventive effect in field is significantly higher than preventive effect when jinggangmycin A is used alone.