CN105660479A - Breeding method for maccullochella peelii - Google Patents
Breeding method for maccullochella peelii Download PDFInfo
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- CN105660479A CN105660479A CN201610093214.7A CN201610093214A CN105660479A CN 105660479 A CN105660479 A CN 105660479A CN 201610093214 A CN201610093214 A CN 201610093214A CN 105660479 A CN105660479 A CN 105660479A
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- 238000009395 breeding Methods 0.000 title abstract description 20
- 241000947101 Maccullochella peelii Species 0.000 title abstract 4
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- 229920000153 Povidone-iodine Polymers 0.000 claims description 8
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- 235000021307 Triticum Nutrition 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 241000415791 Zoothamnium Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000007234 antiinflammatory process Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a breeding method for maccullochella peelii, which adopts the combination of artificial breeding and natural breeding. The breeding method comprises the following steps: 1) preparing a parent fish rearing pond; 2) selecting parent fish; 3) performing antenatal rearing on the parent fish; 4) arranging a roe laying device; 5) performing roe laying and fertilization; and 6) hatching fertilized roes. According to the breeding method for the maccullochella peelii, provided by the invention, each step is simple and convenient to operate, and the fertilization rate (the percentage of the fertilized roes can reach 80-90%) of the roes of the maccullochella peelii and the hatching success rate of the fertilized roes are effectively increased; in addition, the parent fish is not injured in the breeding process, the fertilized roes are effectively prevented from being threatened by diseases such as water molds and the like in the hatching process, the breeding cost is reduced, and the breeding density is increased.
Description
Technical field
The present invention relates to fish reproduction technical field, particularly relate to the propagation method of Australia dragon design speckle.
Background technology
Australia dragon design speckle has special external form and delicious mouthfeel, have " national treasure fish " laudatory title in Australia. Its fine and tender taste thorn delicious, abundant, few, and containing features such as four abundant seed amino acids and EPA and DHA nutrient substance, be one of high-quality Fish. The cultivation of China's Australia dragon design speckle also in the elementary step, only in Zhejiang, Fujian have minority enterprise test cultivation, sell on a small quantity, it does not have be widely popularized, space, domestic and international market is huge.
At present, Australia dragon design speckle generally all adopts artificial propagation, and namely parent fish is hastened parturition and adopts artificial extrusion ovum and sperm to be placed on fertilization bucket and allows its intimate contact be fertilized by injection promoting sexual gland hormone, then artificial incubation germ cell. Although this artificial propagation can control repoductive time as required, improve rate of fertilization. But artificial propagation can make part ovum and sperm congenital defect, and fertilization process is not past natural selection, and Some Constitution is weak, and gene spread to offspring, from generation to generation after work breeding, the bad gene of population genetic gene is many, causes variety deterioration. The most important thing is, Australia dragon design speckle build is bigger, natural disposition is violent, during artificial propagation, it could be carried out squeezing ovum or row's essence by injecting narcotic, very easily damage parent fish, even do not breed additionally, artificial crowded ovum or artificial row's essence are easily caused the fertility decline in upper breeding once of Australia dragon design speckle parent fish. Certainly, the method that Australia dragon design speckle can also adopt natural propagation, but owing to condition is harsh, spawning and fertilization is Artificial Control not easily, causes that rate of fertilization is low.
Therefore, the sapling multiplication method of Australia dragon design speckle, feed nutrition, control and prevention of disease, feeding and management etc. are studied by Fujian Academy of Agricultural Sciences, adopt the method that natural propagation combines with artificial propagation, propose the propagation method of a kind of Australia dragon design speckle, perfect Australia dragon design speckle artificial breeding key technology so that the cultural technique of China's Australia dragon design speckle more steps to a new level.
Summary of the invention
The technical problem to be solved in the present invention, being in that to provide the propagation method of Australia dragon design speckle, the rate of fertilization solving existing Australia dragon design speckle is low, breeding very easily damages parent fish, cause that parent fish fertility declines, gained fry body constitution is weak, easily cause the problems such as variety deterioration.
The present invention is achieved in that the propagation method of Australia dragon design speckle, comprises the following steps:
1) parent fish rearing pond is prepared: prepare the parent fish rearing pond of area 300～600 square metres, at the bottom of service sink and pool wall, and uniformly splash at the bottom of pond with the potassium permanganate solution that concentration is 1～3 g ton water and pool wall, carry out disinfection one day, within second day, change Shui Qingchi, behind clear pond, discharge water 0.8～1.0 meter.It is carried out parent fish rearing pond being possible not only to so that parent fish rearing pond is cleaner, and can effectively kill bacterial micro-organism, reduce parent fish infected by the disease, further working concentration be 1～3 g ton water potassium permanganate solution at the bottom of pond and pool wall carry out thorough sterilizing one day, parent fish is avoided infected by the disease root, certainly, the concentration of potassium permanganate solution to control at 1～3 g ton water within the scope of this, the too low disinfection effect of concentration is inconspicuous, concentration too high one wastes potassium permanganate, improves cost; Two can make the potassium permanganate remained at the bottom of pond and on pool wall be difficult to all clean up. Why discharge water 0.8～1.0 meter, be because 0.8～1.0 meter of this water table ratio and be relatively suitable for the growth and breeding of parent fish.
2) selecting parent fish: annual October, choose 7～15 ages, body weight 10～20 kilograms, female parent fish that fin is complete, and 7～15 ages, body weight 10～20 kilograms, male parent fish that fin is complete, the quantity of male parent fish and female parent fish ratio is for 1:2～4. It is about to begin carries out antenatal cultivation owing to Australia dragon design speckle is typically in December, so needing 1～3 month in advance to select parent fish. Just reaching maturity additionally, Australia dragon design speckle parent fish for breeding needs to cultivate 6 years gonads, so choosing the female parent fish in 7～15 ages respectively, and the male parent fish in 7～15 ages is as the parent fish of the present invention; When parent fish selects, in prioritizing selection the same group, growth is very fast, build is relatively big, build is good, body constitution is healthy and strong, speckle is clear, the high meat of bright in color, the back of the body is thick, fin is complete, without body wound, the nothing lopsided Australia dragon design speckle parent fish as the present invention. In order to ensure the fertility of parent fish, the weight range of male parent fish and female parent fish is all preferred at 10～20 kilograms. Owing to Australia dragon design speckle has milter to protect the behaviors and habits of ovum, milter is more violent in mating period, and milter quantity is too much, it will usually has a fist fight because of contention raun and manor, causes that parent fish is injured; Simultaneously raun lays eggs and will not ovulate in the same time, and ovulation season, therefore, in order to obtain relatively large number of germ cell, when cultivation, the quantity of male parent fish and female parent fish was than being preferably 1:2～4 up to 2 wheat harvesting periods.
3) the antenatal cultivation of parent fish: put the parent fish chosen into parent fish rearing pond, the mixed feed of protein content 45%～55% of throwing something and feeding, parent fish is carried out fortification in 1～3 month; Then controlling water temperature is 6～10 degrees Celsius, parent fish carries out the low temperature stimulation of 2～4 months in this temperature range, makes the gonadal maturation of parent fish; Then water temperature is pressed gradient increased temperature to 18～22 degrees Celsius. Heretofore described mixed feed refers to the mixed feed containing high-quality fish meal, a-starch, fish oil, organic chelated mineral and stable vitamin. Heretofore described refers to, by gradient increased temperature, the fixing temperature that every day water temperature heated up by water temperature, such as just starting temperature is 8 degrees Celsius, water temperature is heated up 1.5 degrees Celsius by every day, and gradient increased temperature 8 days, when the 8th day, water temperature is up to 20 degrees Celsius. Throw something and feed the mixed feed of protein content 45%～55%, parent fish is carried out fortification in 1～3 month, it is ensured that the equilibrium supply of parent fish nutritional need, promote the growth of parent fish gonad well, improve the fertility of parent fish. Additionally, water temperature is to ensure that the key factor of the smooth egg reproduction of parent fish and incubating oosperm. The physiological maturity of Australia dragon design speckle parent fish and the mechanical periodicity of water temperature have very close relationship, certain low temperature cold is needed to stimulate, generally, the physiological maturity of Australia dragon design speckle parent fish gonad is required at the antenatal low temperature stimulation carrying out 2～4 months, it is possible to make parent fish gonadal maturation well.Needing after parent fish gonadal maturation by gradient increased temperature so that water temperature reaches 18～22 degrees Celsius, at this time parent fish just can lay eggs and arrange essence. Certainly, after parent fish gonadal maturation, the intensification of water temperature to be undertaken by gradient, regular, heat up water temperature lentamente, and each increasing extent of temperature can not be excessive, is otherwise easily caused parent fish inadaptable even dead.
4) oopod is set: December is to during February, within the scope of distance pool wall 0～1 meter at the bottom of pond, parent fish rearing pond, placing an oopod every 4～5 meters. December is to during February, parent fish is in the low temperature stimulation stage of antenatal cultivation, time the idiophase of this stage distance parent fish also has some months, at this time parent fish is relatively stable simultaneously, December is selected to be placed in parent fish rearing pond by oopod to February, it is possible to make parent fish first adapt to oopod before idiophase arrives. Owing to Australia dragon design speckle parent fish is liked laying eggs in dark environment during breeding, oopod is arranged within the scope of distance pool wall 0～1 meter at the bottom of pond, parent fish rearing pond, namely near the place of pool wall, it is possible to be effectively improved the spawning rate of Australia dragon design speckle parent fish. Dragon design speckle parent fish has the behaviors and habits protecting ovum in Australia, more violent in mating period, it will usually has a fist fight because of contention manor, so oopod to keep the interval of 4～5 meters, can guarantee that parent fish will not be had a fist fight, cause that parent fish is injured.
5) lay eggs and be fertilized: water temperature being maintained at 20～22 degrees Celsius 25～40 days, making parent fish oopod in parent fish rearing pond to be laid eggs and is fertilized. The propagation method of Australia of the present invention dragon design speckle adopts natural propagation method, i.e. pairing naturally, natural spawning, natural fertilization, do not need stimulation by running water and artificial induced spawning, propagation method is relatively simple, the suitable water temperature of Australia dragon design speckle breeding at 20～22 degrees Celsius, as long as water temperature stability 20～22 degrees Celsius about one week, parent fish just can carry out beginning natural spawning, natural fertilization, after 25～40 days, major part parent fish just can complete to lay eggs and be fertilized.
Table 1 parent fish spontaneous ovulation natural fertilization log
In addition; owing to Australia dragon design speckle parent fish has the behaviors and habits protecting ovum; more violent in mating period; would generally have a fist fight because of contention manor; so treating Gunther parent fish spawning or after fertilization, for injured parent fish, injured parent fish is carried out antiinflammatory process by the dysentery bacterium timely concentration to be 2～3 g ton water aqueous solution that disappears; and the healing of parent fish wound, it is prevented that parent fish wound infection disease and cause death.
6) hatching of germ cell: the germ cell taken out in oopod is hatched to hatchery, and hatching density is 2～50,000/cubic metre; Water temperature is maintained at 20～22 degrees Celsius, aeration oxygen replenishing, Lotic hatching, and discharge is 50～100 ls/h, obtains Australia dragon design speckle fry after hatching 5～8 days. The hatchery of the present invention and the isolation of parent fish rearing pond, it is possible to be arranged close to side, parent fish antenatal cultivation pond, it is ensured that germ cell can be transferred in hatchery rapidly from oopod. The reasonable hatching density of Australia of the present invention dragon design speckle germ cell is 2～50,000/cubic metre, it is possible to guaranteeing to hold on the basis of enough dissolved oxygen abundant germ cell. And temperature controls in 20～22 degree Celsius range, the hatching effect of Australia dragon design speckle germ cell is best, now aeration oxygen replenishing, and the water flow velocity with flow for 50～100 ls/h, can be effectively improved the incubation rate of germ cell simultaneously.After Australia dragon design speckle fry all hatches out, the screen cloth in hatchery is taken out, vacates more space to fry, allow fry support 5～7 days temporarily in hatchery.
Table 2: parent fish spontaneous ovulation artificial incubation log
7) prevention of disease and treatment: with the potassium permanganate solution that concentration is 1～3 g ton water at the bottom of pond, parent fish rearing pond and pool wall carry out that pond is cleared and sterilized; Successively parent fish is carried out disinfection with the dysentery bacterium of the povidone iodine aqueous solution that concentration is 6 g ton water and 2～3 g ton water aqueous solution that disappears; By the fish health and happiness of the glutaraldehyde of 1～3 g ton water and 1～2 g ton water to sick fish soaking 5 days; By 10% florfenicol of 0.5～1 g ton water, pre-bacteriological protection complication.
With the potassium permanganate solution that concentration is 1～3 g ton water at the bottom of pond and pool wall carry out thorough sterilizing one day, parent fish is avoided infected by the disease root, certainly, the concentration of potassium permanganate solution to control at 1～3 g ton water within the scope of this, the too low disinfection effect of concentration is inconspicuous, the too high potassium permanganate of not only wasting of concentration, improves cost; The potassium permanganate remained at the bottom of pond and on pool wall also can be made to be difficult to all clean up.
Concentration is that the povidone iodine aqueous solution of 6 g ton water is in order to kill virus, antibacterial, fungus and the mycotic spore dewatering in body. Concentration is that the dysentery bacterium of 2～3 g ton water disappears aqueous solution for the antiinflammatory of parent fish wound and healing.
By the fish health and happiness of the glutaraldehyde of 1～3 g ton water and 1～2 g ton water to sick fish soaking 5 days, it is possible to effectively treat Australia dragon design pinta evil that ichthyophthiriasis and chilodoniasis etc. are common. Can adding antibiotic when parent fish introduced disease is more serious, such as 10% florfenicol of 0.5～1 g ton water, pre-bacteriological protection complication occurs.
In the dragon design speckle breeding process of Australia, Major Diseases includes fish molds, trichodinasis, ichthyophthiriasis, chilodoniasis, shield balantidiasis, Disease of Zoothamnium, bacterial gill rot disease, bacillary tail-rot disease, viral disease. In the propagation method of Australia dragon design speckle of the present invention with the potassium permanganate solution that concentration is 1～3 g ton water at the bottom of pond, parent fish rearing pond and pool wall carry out that pond is cleared and sterilized, it is ensured that breeding clean environment, it is therefore prevented that Australia dragon design speckle suffers the infection of disease or antibacterial. If additionally, Australia dragon design speckle obtained the disease such as ichthyophthiriasis or chilodoniasis in reproductive process, it is possible to by the fish health and happiness of the glutaraldehyde of 1～3 g ton water and 1～2 g ton water to sick fish soaking 5 days, parent fish can be cured; And for ill more serious parent fish, it is possible to 10% florfenicol of 0.5～1 g ton water, parent fish is treated, pre-bacteriological protection complication.
Certainly, in order to prevention and control disease better occurs and prevents and treats timely in the dragon design speckle reproductive process of Australia, application prophylaxis to be focused on, focus on distinguishing and diagnosis of disease, implement effectively preventing simultaneously. This process want treatment in accordance with local conditions formulate therapeutic scheme, can effectively prevention and control, can prevent the pollution of the environment again. Therefore, the cultural method of the present invention additionally provides 9 kinds of commonly encountered diseases diagnostic methods in the propagation method process of Australia dragon design speckle, preventive measure, therapeutic scheme and general condensed summary etc., lists among table 3 below.
The diagnostic method of Major Diseases and preventive measure in the dragon design speckle breeding process of table 3 Australia
Further, step 6) the germ cell hatching process of 5～8 days specifically includes: the dipped into formalin germ cell half an hour that first 4 day every day adopted concentration to be 0.1%, it is prevented that fish molds grows on dead ovum; Within 5th day, fry starts hatching, stops using dipped into formalin;Within 8th day, take out screen cloth, make fry support temporarily in hatchery. Concentration be 0.1% formalin solution can effectively kill the bacterial propagule in hatchery, brood cell's (such as anthrax spores), tubercule bacillus or virus etc.
Further, step 2) in female parent fish and male parent fish, choose the female first filial generation of wild Australia dragon design speckle artificial culture and male first filial generation respectively as female parent fish and male parent fish. Wild Australia dragon design speckle body constitution is healthy and strong, it is thick to carry on the back high meat, fertility is strong, but wild Australia dragon design speckle stock number reduces day by day, and the quantity catching Australia dragon design speckle parent fish is few especially; Additionally, wild Australia dragon design speckle is tamed, difficulty is big, cost is high. Therefore the present invention selects the female first filial generation of wild Australia dragon design speckle artificial culture and male first filial generation as female parent fish and male parent fish, it can be ensured that parent fish has good gene and fertility.
Further, step 1) in, the water surface in parent fish rearing pond is configured with the paddle aerator for blowdown and oxygen supplement. Paddle aerator drives the rotation of water surface impeller to churn the surface, stir air film and liquid film by motor, increases the contact area of air-liquid, to expand oxygen Concentraton gradient in water, promotes the oxygen in air and shifts the speed of diffusion in water. Additionally, paddle aerator is in the process churned the surface, water being made to carry out sluggish flow, current can take the Excreta of feedstuff remaining in water body or Australia dragon design speckle to sewage draining exit, such that it is able to be effectively improved water body environment.
Further, step 3) pond, parent fish rearing pond at the bottom of four corner locations be equipped with aeration oxygen replenishing pipe. Aeration oxygen replenishing pipe is arranged on four corners in parent fish rearing pond, it is ensured that the oxygen that aeration oxygen replenishing pipe provides can be fully contacted with the water in parent fish rearing pond, thus the dissolved oxygen amount increased in liquid, to ensure the growth demand of Australia dragon design speckle parent fish.
Further, step 4) in oopod be that outer surface is coated with the hollow cylinder oopod being provided with screen cloth on the bottom surface of tilt, both ends open, inner surface. Carry out at lucifuge shady place owing to being usually time Australia dragon design speckle is laid eggs, so the outer surface of oopod needs to be coated with tilt; Oopod both ends open is the import and export of oopod.
Further, described oopod both ends open place is provided with highlight regions; The screen cloth arranged on the bottom surface of the inner surface of oopod body and oopod body removably connect. The two-port place of hollow cylinder is provided with highlight regions, just can so that Fish be when laying eggs, carry out at the middle part of hollow cylinder, greatly reduce at hollow cylinder end egg laying amount, also being considerably reduced the quantity that port fish roe drops when extracting, taking, from oopod body, the loss caused ovum process thus reducing. Additionally, due to screen cloth and oopod body are for removably connecting, thus easily screen cloth can be taken out from oopod body, substantially increase and take ovum efficiency, also reduce corresponding cost of labor.
Further, step 3) described in mixed feed be granular mixed feed, the composition of described granular mixed feed includes high-quality fish meal, a-starch, fish oil, organic chelated minerals and vitamins. Ensure that the equilibrium supply of parent fish nutritional need, effectively promote parent fish gonad development.
Further, step 3) the water condition of the antenatal cultivation of parent fish be: acid-base value 7.0～8.0, ammonia-nitrogen content is less than 0.2 mg/litre.Australia dragon design speckle cultivation generally requires that in water body, ammonia-nitrogen content is less than 0.2 mg/litre, the highest less than 0.2 mg/litre. Ammonia-nitrogen content is once too high, it is easy to bring out disease, betides water body ammonia nitrogen solubility height correlation such as Australia dragon design speckle streptococcicosis.
Further, step 6) described in hatchery material be glass material, described hatchery is bottom area 2～5 square metres, the cuboid of high 0.5 meter. The hatchery of glass material, glass material inner wall smooth on the one hand, germ cell will not be caused damage, facilitate the hatching situation of Australia dragon design speckle germ cell in staff's Real Time Observation hatchery on the other hand, find and process germ cell in time and infect the emergencies such as water mo(u)ld, avoid the bad ovum infecting water mo(u)ld to infect the germ cell of health, cause the infected water mo(u)ld of more germ cell and dead.
The propagation method of Australia dragon design speckle of the present invention has the advantage that each step operation is simple and convenient, it is effectively increased the rate of fertilization (rate of fertilization is up to 80%～90%) of Australia dragon design speckle fish roe and the hatching success (hatching success is up to more than 90%) of germ cell, reproductive process will not be damaged parent fish, effectively prevent incubating oosperm process and threatened by diseases such as fish moldss, reduce aquaculture cost and improve cultivation density.
Detailed description of the invention
By describing the technology contents of the present invention, structural feature in detail, being realized purpose and effect, it is explained in detail below in conjunction with embodiment.
Embodiment one:
1) parent fish rearing pond is prepared: prepare the parent fish rearing pond of area 300 square metres, at the bottom of service sink and pool wall, and uniformly splash at the bottom of pond with the potassium permanganate solution that concentration is 1 g ton water and pool wall, carry out disinfection one day, within second day, change Shui Qingchi, behind clear pond, discharge water 0.8 meter;
2) selecting parent fish: annual October, choose 7 ages, body weight 10～15 kilograms, female parent fish that fin is complete, and 7 ages, body weight 10～15 kilograms, male parent fish that fin is complete, the quantity of male parent fish and female parent fish ratio is for 1:2;
3) the antenatal cultivation of parent fish: put the parent fish chosen into parent fish rearing pond, the mixed feed of protein content 45% of throwing something and feeding, parent fish is carried out the fortification of 1 month; Then controlling water temperature is 6 degrees Celsius, parent fish carries out the low temperature stimulation of 2 months in this temperature range, makes the gonadal maturation of parent fish; Then water temperature is pressed gradient increased temperature to 18～20 degrees Celsius;
4) oopod is set: December, contacting pool wall place at the bottom of pond, parent fish rearing pond, places an oopod every 4 meters;
5) lay eggs and be fertilized: water temperature being maintained at 20～22 degrees Celsius 25 days, making parent fish oopod in parent fish rearing pond to be laid eggs and is fertilized;
6) hatching of germ cell: the germ cell taken out in oopod is hatched to hatchery, and hatching density is 20,000/cubic metre; Water temperature is maintained at 20 degrees Celsius, aeration oxygen replenishing, Lotic hatching, and discharge is 50/ hour, obtains Australia dragon design speckle fry after hatching 5～8 days;
7) prevention of disease and treatment: with the potassium permanganate solution that concentration is 1 g ton water at the bottom of pond, parent fish rearing pond and pool wall carry out that pond is cleared and sterilized; Successively parent fish is carried out disinfection with the dysentery bacterium of the povidone iodine aqueous solution that concentration is 6 g ton water and the 2 g ton water aqueous solution that disappears; By the fish health and happiness of the glutaraldehyde of 1 g ton water and 1 g ton water to sick fish soaking 5 days; By 10% florfenicol of 0.5 g ton water, pre-bacteriological protection complication.
Embodiment two:
1) parent fish rearing pond is prepared: prepare the parent fish rearing pond of area 400 square metres, at the bottom of service sink and pool wall, and uniformly splash at the bottom of pond with the potassium permanganate solution that concentration is 1.5 g ton water and pool wall, carry out disinfection one day, within second day, change Shui Qingchi, behind clear pond, discharge water 0.8 meter;
2) selecting parent fish: annual October, choose 9 ages, body weight 10～15 kilograms, female parent fish that fin is complete, and 9 ages, body weight 10～15 kilograms, male parent fish that fin is complete, the quantity of male parent fish and female parent fish ratio is for 1:3;
3) the antenatal cultivation of parent fish: put the parent fish chosen into parent fish rearing pond, the mixed feed of protein content 47% of throwing something and feeding, parent fish is carried out the fortification of 1 first quarter moon; Then controlling water temperature is 7 degrees Celsius, parent fish carries out the low temperature stimulation of two first quarter moons in this temperature range, makes the gonadal maturation of parent fish; Then water temperature is pressed gradient increased temperature to 19 degrees Celsius;
4) oopod is set: during January, at distance pool wall 0.3 meter at the bottom of pond, parent fish rearing pond, places an oopod every 4 meters;
5) lay eggs and be fertilized: water temperature being maintained at 21 degrees Celsius 30 days, making parent fish oopod in parent fish rearing pond to be laid eggs and is fertilized;
6) hatching of germ cell: the germ cell taken out in oopod is hatched to hatchery, and hatching density is 30,000/cubic metre; Water temperature is maintained at 21 degrees Celsius, aeration oxygen replenishing, Lotic hatching, and discharge is 60 ls/h, obtains Australia dragon design speckle fry after hatching 5～8 days;
7) prevention of disease and treatment: with the potassium permanganate solution that concentration is 1.5 g ton water at the bottom of pond, parent fish rearing pond and pool wall carry out that pond is cleared and sterilized; Successively parent fish is carried out disinfection with the dysentery bacterium of the povidone iodine aqueous solution that concentration is 6 g ton water and the 2.2 g ton water aqueous solution that disappears; By the fish health and happiness of the glutaraldehyde of 1.5 g ton water and 1.2 g ton water to sick fish soaking 5 days; By 10% florfenicol of 0.6 g ton water, pre-bacteriological protection complication.
Embodiment three:
1) parent fish rearing pond is prepared: prepare the parent fish rearing pond of area 450 square metres, at the bottom of service sink and pool wall, and uniformly splash at the bottom of pond with the potassium permanganate solution that concentration is 2 g ton water and pool wall, carry out disinfection one day, within second day, change Shui Qingchi, behind clear pond, discharge water 0.9 meter;
2) selecting parent fish: annual October, choose 11 ages, body weight 13～18 kilograms, female parent fish that fin is complete, and 11 ages, body weight 13～18 kilograms, male parent fish that fin is complete, the quantity of male parent fish and female parent fish ratio is for 1:3;
3) the antenatal cultivation of parent fish: put the parent fish chosen into parent fish rearing pond, the mixed feed of protein content 50% of throwing something and feeding, parent fish is carried out fortification in 2 months; Then controlling water temperature is 8 degrees Celsius, parent fish carries out the low temperature stimulation of 2 months in this temperature range, makes the gonadal maturation of parent fish; Then water temperature is pressed gradient increased temperature to 21 degrees Celsius;
4) oopod is set: December is to during February, within the scope of distance pool wall 0～1 meter at the bottom of pond, parent fish rearing pond, placing an oopod every 4～5 meters;
5) lay eggs and be fertilized: water temperature being maintained at 20～22 degrees Celsius 25～40 days, making parent fish oopod in parent fish rearing pond to be laid eggs and is fertilized;
6) hatching of germ cell: the germ cell taken out in oopod is hatched to hatchery, and hatching density is 2～50,000/cubic metre; Water temperature is maintained at 20～22 degrees Celsius, aeration oxygen replenishing, Lotic hatching, and discharge is 50～100 ls/h, obtains Australia dragon design speckle fry after hatching 5～8 days;
7) prevention of disease and treatment: with the potassium permanganate solution that concentration is 2 g ton water at the bottom of pond, parent fish rearing pond and pool wall carry out that pond is cleared and sterilized; Successively parent fish is carried out disinfection with the dysentery bacterium of the povidone iodine aqueous solution that concentration is 6 g ton water and the 2.5 g ton water aqueous solution that disappears;By the fish health and happiness of the glutaraldehyde of 2 g ton water and 1.5 g ton water to sick fish soaking 5 days; By 10% florfenicol of 0.75 g ton water, pre-bacteriological protection complication.
Embodiment four:
1) parent fish rearing pond is prepared: prepare the parent fish rearing pond of area 500 square metres, at the bottom of service sink and pool wall, and uniformly splash at the bottom of pond with the potassium permanganate solution that concentration is 2.5 g ton water and pool wall, carry out disinfection one day, within second day, change Shui Qingchi, behind clear pond, discharge water 1.0 meters;
2) selecting parent fish: annual October, choose 13 ages, body weight 14～19 kilograms, female parent fish that fin is complete, and 13 ages, body weight 14～19 kilograms, male parent fish that fin is complete, the quantity of male parent fish and female parent fish ratio is for 1:3;
3) the antenatal cultivation of parent fish: put the parent fish chosen into parent fish rearing pond, the mixed feed of protein content 45%～55% of throwing something and feeding, parent fish is carried out fortification in 2 months; Then controlling water temperature is 9 degrees Celsius, parent fish carries out the low temperature stimulation of 2～4 months in this temperature range, makes the gonadal maturation of parent fish; Then water temperature is pressed gradient increased temperature to 21 degrees Celsius;
4) oopod is set: December is to during February, at distance 0.8 meter of of pool wall at the bottom of pond, parent fish rearing pond, every one oopod of 5 meters of placements;
5) lay eggs and be fertilized: water temperature being maintained at 21 degrees Celsius 35 days, making parent fish oopod in parent fish rearing pond to be laid eggs and is fertilized;
6) hatching of germ cell: the germ cell taken out in oopod is hatched to hatchery, and hatching density is 40,000/cubic metre; Water temperature is maintained at 21 degrees Celsius, aeration oxygen replenishing, Lotic hatching, and discharge is 80 ls/h, obtains Australia dragon design speckle fry after hatching 5～8 days;
7) prevention of disease and treatment: with the potassium permanganate solution that concentration is 2.5 g ton water at the bottom of pond, parent fish rearing pond and pool wall carry out that pond is cleared and sterilized; Successively parent fish is carried out disinfection with the dysentery bacterium of the povidone iodine aqueous solution that concentration is 6 g ton water and the 2.8 g ton water aqueous solution that disappears; By the fish health and happiness of the glutaraldehyde of 2.6 g ton water and 1.8 g ton water to sick fish soaking 5 days; By 10% florfenicol of 0.9 g ton water, pre-bacteriological protection complication.
Embodiment five:
1) parent fish rearing pond is prepared: prepare the parent fish rearing pond of area 600 square metres, at the bottom of service sink and pool wall, and uniformly splash at the bottom of pond with the potassium permanganate solution that concentration is 3 g ton water and pool wall, carry out disinfection one day, within second day, change Shui Qingchi, behind clear pond, discharge water 1.0 meters;
2) selecting parent fish: annual October, choose 15 ages, body weight 15～20 kilograms, female parent fish that fin is complete, and 15 ages, body weight 14～19 kilograms, male parent fish that fin is complete, the quantity of male parent fish and female parent fish ratio is for 1:4;
3) the antenatal cultivation of parent fish: put the parent fish chosen into parent fish rearing pond, the mixed feed of protein content 55% of throwing something and feeding, parent fish is carried out fortification in 3 months; Then controlling water temperature is 10 degrees Celsius, parent fish carries out the low temperature stimulation of 3 months in this temperature range, makes the gonadal maturation of parent fish; Then water temperature is pressed gradient increased temperature to 22 degrees Celsius;
4) oopod is set: December is to during February, at distance 1 meter of of pool wall at the bottom of pond, parent fish rearing pond, every one oopod of 5 meters of placements;
5) lay eggs and be fertilized: water temperature being maintained at 20～22 degrees Celsius 40 days, making parent fish oopod in parent fish rearing pond to be laid eggs and is fertilized;
6) hatching of germ cell: the germ cell taken out in oopod is hatched to hatchery, and hatching density is 50,000/cubic metre; Water temperature is maintained at 22 degrees Celsius, aeration oxygen replenishing, Lotic hatching, and discharge is 100 ls/h, obtains Australia dragon design speckle fry after hatching 5～8 days;
7) prevention of disease and treatment: with the potassium permanganate solution that concentration is 3 g ton water at the bottom of pond, parent fish rearing pond and pool wall carry out that pond is cleared and sterilized; Successively parent fish is carried out disinfection with the dysentery bacterium of the povidone iodine aqueous solution that concentration is 6 g ton water and 2～3 g ton water aqueous solution that disappears; By the fish health and happiness of the glutaraldehyde of 3 g ton water and 2 g ton water to sick fish soaking 5 days; By 10% florfenicol of 1 g ton water, pre-bacteriological protection complication.
The foregoing is only embodiments of the invention; not thereby the scope of patent protection of the present invention is limited; every equivalent structure utilizing description of the present invention to make or equivalence flow process conversion; or directly or indirectly it is used in other relevant technical fields, all in like manner include in the scope of patent protection of the present invention.
Claims (10)
1. the propagation method of Australia dragon design speckle, it is characterised in that comprise the following steps:
1) parent fish rearing pond is prepared: prepare the parent fish rearing pond of area 300～600 square metres, at the bottom of service sink and pool wall, and uniformly splash at the bottom of pond with the potassium permanganate solution that concentration is 1～3 g ton water and pool wall, carry out disinfection one day, within second day, change Shui Qingchi, behind clear pond, discharge water 0.8～1.0 meter;
2) selecting parent fish: annual October, choose 7～15 ages, body weight 10～20 kilograms, female parent fish that fin is complete, and 7～15 ages, body weight 10～20 kilograms, male parent fish that fin is complete, the quantity of male parent fish and female parent fish ratio is for 1:2～4;
3) the antenatal cultivation of parent fish: put the parent fish chosen into parent fish rearing pond, the mixed feed of protein content 45%～55% of throwing something and feeding, parent fish is carried out fortification in 1～3 month; Then controlling water temperature is 6～10 degrees Celsius, parent fish carries out the low temperature stimulation of 2～4 months in this temperature range, makes the gonadal maturation of parent fish; Then water temperature is pressed gradient increased temperature to 18～22 degrees Celsius;
4) oopod is set: December is to during February, within the scope of distance pool wall 0～1 meter at the bottom of pond, parent fish rearing pond, placing an oopod every 4～5 meters;
5) lay eggs and be fertilized: water temperature being maintained at 20～22 degrees Celsius 25～40 days, making parent fish oopod in parent fish rearing pond to be laid eggs and is fertilized;
6) hatching of germ cell: the germ cell taken out in oopod is hatched to hatchery, and triploidy rate is 2～50,000/cubic metre; Water temperature is maintained at 20～22 degrees Celsius, aeration oxygen replenishing, Lotic hatching, and discharge is 50～100 ls/h, obtains Australia dragon design speckle fry after hatching 5～8 days.
7) prevention of disease and treatment: with the potassium permanganate solution that concentration is 1～3 g ton water at the bottom of pond, parent fish rearing pond and pool wall carry out that pond is cleared and sterilized; Successively parent fish is carried out disinfection with the dysentery bacterium of the povidone iodine aqueous solution that concentration is 6 g ton water and 2～3 g ton water aqueous solution that disappears; By the fish health and happiness of the glutaraldehyde of 1～3 g ton water and 1～2 g ton water to sick fish soaking 5 days; By 10% florfenicol of 0.5～1 g ton water, pre-bacteriological protection complication.
2. the propagation method of Australia according to claim 1 dragon design speckle, it is characterized in that: step 6) the germ cell hatching process of 5～8 days specifically includes: the dipped into formalin germ cell half an hour that first 4 day every day adopted concentration to be 0.1%, it is prevented that fish molds grows on dead ovum;Within 5th day, fry starts hatching, stops using dipped into formalin; Within 8th day, take out screen cloth, make fry support temporarily in hatchery.
3. the propagation method of Australia according to claim 1 dragon design speckle, it is characterized in that: step 2) in female parent fish and male parent fish, choose the female first filial generation of wild Australia dragon design speckle artificial culture and male first filial generation respectively as female parent fish and male parent fish.
4. the propagation method of Australia according to claim 1 dragon design speckle, it is characterised in that: step 1) in, the water surface in parent fish rearing pond is configured with the paddle aerator for blowdown and oxygen supplement.
5. the propagation method of Australia according to claim 1 dragon design speckle, it is characterised in that: step 3) pond, parent fish rearing pond at the bottom of four corner locations be equipped with aeration oxygen replenishing pipe.
6. the propagation method of Australia according to claim 1 dragon design speckle, it is characterised in that: step 4) in oopod be that outer surface is coated with the hollow cylinder oopod being provided with screen cloth on the bottom surface of tilt, both ends open, inner surface.
7. the propagation method of Australia according to claim 6 dragon design speckle, it is characterised in that: described oopod both ends open place is provided with highlight regions; The screen cloth arranged on the bottom surface of the inner surface of oopod body and oopod body removably connect.
8. the propagation method of Australia according to claim 1 dragon design speckle, it is characterized in that: step 3) described in mixed feed be granular mixed feed, the composition of described granular mixed feed includes fish flour, a-starch, fish oil, organic chelated minerals and vitamins.
9. the propagation method of Australia according to claim 1 dragon design speckle, it is characterised in that: step 3) the water condition of the antenatal cultivation of parent fish be: acid-base value 7.0～8.0, ammonia-nitrogen content is less than 0.2 mg/litre.
10. the propagation method of Australia according to claim 1 dragon design speckle, it is characterised in that: step 6) described in hatchery material be glass steel material, described hatchery is bottom area 2～5 square metres, the cuboid of high 0.5 meter.
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Effective date of registration: 20231121 Address after: Room 772, Entrepreneurship Building, Entrepreneurship Park, Torch High tech Zone, Xiamen City, Fujian Province, 361115 Patentee after: Xiamen Maixin Zhinong Wulian Technology Co.,Ltd. Address before: 350000 No. 54, 247 Gulou District, Fujian, Fuzhou Patentee before: INSTITUTE OF QUALITY STANDARD AND TESTING TECHNOLOGY FOR AGRO-PRODUCTS, FUJIAN ACADEMY OF AGRICULTURAL SCIENCES |
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